dme-mir-974- Best orthologs

dme-mir-974	chrX:19445711-19445811 +	Known_mirbase
[View on miRBase]	[View on UCSC Genome Browser {Cornell Mirror}]	intergenic

Legend:

   mature strand at position 19445735-19445757
   star strand at position 19445775-19445796
   mutations
   read mismatches

Notes: "chrX:11,892,000-11,893,400 yakuba gap was resequenced; found the 973 and 974 ortholog. Did not find the 972 ortholog. Would be good to resequence this region. droBip1 ortholog is reported in two pieces. DroGri2 may also be in two pieces."

20-way Fast Statistical Aligner (FSA) re-alignment

Visually pleasing best hits from LASTZ and MAF

Species	Coordinate	Source	Confidence	Alignment
dm3	chrX:19445696-19445826 +		1	AGGTCCAGGTAAGC---------------TCAAT-----TGT-----------CACCGGTCATGTCCTCCAAGCGAGCAAAGAAGTAGTATTTGTGTTT--C--CAA--GAGCAAATATAACTTCATTGGAA-GCTAAGTGGATTTGCCCA-AATCA-------------GCTTG-GT-ATTT------------------------------------------------------AT
droSim1	chrX:15011423-15011546 +	LASTZ	1	GTAA--------AC---------------TCAAT-----TGT-----------CACTGGTCGTGTCCTCCAAGCGAGCAAAGAAGTGGTATTTGCGTTT--C--CAA--GAACAAATATAACTTCTTTGAAA-GCTAGGTGGATTTGCCCA-AGTCA-------------CCTTG-GT-ATTT------------------------------------------------------AT
droSec1	super_8:1728102-1728227 +	LASTZ	1	AGGTAA------AC---------------TCGAT-----TGT-----------CACTGGTCGTGTCCTCCAAGCGAGCAAAGAAGTAGTATTTGCGTTT--C--CAA--GAACAAATATAACTTCTTTGAAA-GCTAGGTGGATTTGCCCA-AGTCT-------------CCTTG-GT-ATTT------------------------------------------------------AT
droYak2	Unknown	MAF	1	-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEre2	scaffold_4690:9682437-9682562 +	LASTZ	1	GAAAACAGAACCAC---------------TAACT-----CAG-----------CACTGGGCATGTCCTCCAAGCGAGCAAAGAAGTAGTATTTGTGTTC--C--AAA--AGTCAAATATAACTTTTTTGGAA-GCTACGGGGATTTGCCCA-AGCCA-------------ACTTG-CT-G-----------------------------------------------------------
droEug1	scf7180000409109:6699-6831 -	LASTZ	4	AAACATAAT--AAT-----TAAGA-----TTAAG-----AAT-----------AATTGGATATGTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTAT------TA--AGTCGAATATAACTTTTTTGGAA-GCTAAGCGGATGTATCTAAACCCA-------------GCTTG-AT-GTTT------------------------------------------------------TT
droBia1	scf7180000302069:1157324-1157449 -	LASTZ	4	AAGGCAATACAAAT----TTAG--------AATT-----TAG-----------AAAAGGTCATCTCCTCCAAGCGAGCAAAGAAGTAGTATTTGTTTGT--T--AAA--AGTCAAATATAACTTTTTTGGAA-GCTAAGAGGATTTATCCT-AACCA-------------GCTTG----------------------------------------------------------------
droTak1	scf7180000415169:850584-850710 -	LASTZ	4	ATTTGGA------------------------ACT-----TCG-----------AATAGGTCATCTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTGT--T-GAAG--AGTCAAATATAACTTTTTTGGAA-GCTAAGAGGATTTGTCCT-AAGCA-------------GCTTG-GTTATTTCGA---------------------------------------------------AT
droEle1	scf7180000490751:1106991-1107176 +	LASTZ	4	AAATACGG----GC---------------TGACT-----CGG-----------AAAAGGTCATTTCCTCCAAGCGAGCAAAGAAGTAGTATTTGTTTGT-TCTGAAA--AGTCAAATATAACTTTTTTGAAA-GCTACGAGGATTTGTCCCAAATCA-------------CG-TGCATTGTTTTTGAGCGAAAGACCAGGACGGATGGAAAATCAGGAGCAAAACCGTGAGAAACAAGT
droRho1	scf7180000761121:198567-198704 -	LASTZ	4	AACGA-------GC---------------TTTCT-----CGA-----------AATAGGTCATCTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTGT-TCTGAAA--AGTCAAATATAACTTTTTTGAAA-GCTACGAGGATTTGTTCC-ATTAACAATAAAAGCA--GTGAA-AG-CCAT------------------------------------------------------AG
droFic1	scf7180000454073:1724056-1724193 +	LASTZ	4	AGTGCTAAGGATACGCAATTAA--------AAAC-----AAA-----------AAAGGAGCATTTCCCCTAAGCGAGCAAAGAAGTAGTATTTGTTCGT-GCTGAAA--ACTCAAATATAACTTTTTTGAAA-GCTAAAAGGATTTGTTTGCAATCA-------------GCT------GCAT------------------------------------------------------TT
droKik1	scf7180000302517:1176347-1176493 +	LASTZ	4	ATAGCCGGGACATC---------------TC-CG-----CCA-----------AGCTGGTCTTTTCCTCTAAGCGAGCAAAGAAGTGGTATTTGTTGCA-CTTGAAATTATTCAAATATAACTTTTTTGGAA-GCTAGAAGGAGTTGCCCACAAGCA-----CACTTTAATCCCA-AT-ATTTCTC---------------------------------------------------AG
droAna3	scaffold_13417:2478487-2478598 -	LASTZ	1	GGTTGCAA----T--------------CCTGGCT-----TGG-----------GGTAGGTCATCTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTGC-TTTTAAT--GGTCAAATATAACTTTTTTGGAA-GCTACGAGGAGTTGTCC-----------------------------------------------------------------------------------------
droBip1	scf7180000395874:49590-49667 -	LASTZ	0	ATCCCGAACCAATCGCGGTTGC--AATCC-----------TTGGGTGGATTGGGGTAGGTCATCTCCTCTAAGCGAGCAAAGAAGGCGTAT----------------------------------------------------------------------------------------------------------------------------------------------------
dp4	Unknown		0	-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droPer1	Unknown		0	-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droWil1	scaffold_181096:7946609-7946719 +	LASTZ	1	ATTCAT--------------------------TT-----TTC-----------CAATGGCCATATCCTCTAAGCGAGCAAGAAAGTGGTATTTGTCTTT--G--GAA--ATTCAAATATAACTTTTTTGGAA-GCTACAAGGCAATGTCCACAATCA-------------AC-------------------------------------------------------------------
droVir3	scaffold_13042:917533-917679 -	LASTZ	1	ATCCGAATATGAGA---------------TTTACCAATTATT-----------AACTGGGCATTCCCTCTGAGCGAGCAGAGAAGTATTATTTGCGTGAATC--TGA--TATCAAATAAGACTTTTTTGGTTCGCTATGAGGTATTGTTCATAAATA-----AACACG--TCTGC-CT-ATAT------------------------------------------------------AC
droMoj3	scaffold_6328:2908489-2908614 -	LASTZ	1	TCAATC---------------------------------AGA-----------CGCTGGGCATTCCCTCTGAGCGAGCAGAGAAGTATTTTT-GCGTGCATC--TGA--TATCAAATAAAACTTTTTTGGTT-GCTGCGAGGTATTGTTGA-AATGT----AAACGTT--GCTCG-CT-CTCT------------------------------------------------------AC
droGri2	scaffold_15081:2051206-2051247 -	LASTZ	5	CA-----------------------------------------------------AAGGACATCTTCTGTAAGCGAGCAAAAATATAATATTTGT------------------------------------------------------------------------------------------------------------------------------------------------

Legend

0- The ortholog is absent. A better synthenic sequence candidate could not be found.
1- Reads w/ good pileup on mature and star strand & folds into hairpin structure.
2- Reads w/ good pileup on mature and star strand BUT does not fold into hairpin structure.
3- Sporadic reads & folds into a hairpin structure.
4- No reads but folds into a hairpin structure.
5- Ortholog is confident but miRNA appears absent (no reads and does not fold into a hairpin)

Re-alignment after removal of non-confident orthologs

Species	Alignment
dm3	AGGTCCAGGTA-----AGC-----T---CAATTGTCACCGGTCATGTCCTCCAAGCGAGCAAAGAAGTAGTATTTGTGTTT--C--CAA--GAGCAAATATAACTTCATTGGAA-GCTAAGTGGATTTGCCCA-AATCA-------------GCTTGGT-ATTT------------------------------------------------------AT
droSim1	GTAAA-------------C-----T---CAATTGTCACTGGTCGTGTCCTCCAAGCGAGCAAAGAAGTGGTATTTGCGTTT--C--CAA--GAACAAATATAACTTCTTTGAAA-GCTAGGTGGATTTGCCCA-AGTCA-------------CCTTGGT-ATTT------------------------------------------------------AT
droSec1	AGGTA-----------AAC-----T---CGATTGTCACTGGTCGTGTCCTCCAAGCGAGCAAAGAAGTAGTATTTGCGTTT--C--CAA--GAACAAATATAACTTCTTTGAAA-GCTAGGTGGATTTGCCCA-AGTCT-------------CCTTGGT-ATTT------------------------------------------------------AT
droYak2	----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEre2	GAAAACAGAAC-----CAC-----T---AACTCAGCACTGGGCATGTCCTCCAAGCGAGCAAAGAAGTAGTATTTGTGTTC--C--AAA--AGTCAAATATAACTTTTTTGGAA-GCTACGGGGATTTGCCCA-AGCCA-------------ACTTGCT-G-----------------------------------------------------------
droEug1	AAACATA--AT-----AAT-TA--AGATTAAGAATAATTGGATATGTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTAT------TA--AGTCGAATATAACTTTTTTGGAA-GCTAAGCGGATGTATCTAAACCCA-------------GCTTGAT-GTTT------------------------------------------------------TT
droBia1	AAGGC-A----ATACAAATTTA--GA---ATTTAGAAAAGGTCATCTCCTCCAAGCGAGCAAAGAAGTAGTATTTGTTTGT--T--AAA--AGTCAAATATAACTTTTTTGGAA-GCTAAGAGGATTTATCCT-AACCA-------------GCTTG---------------------------------------------------------------
droTak1	ATTTG-------------------GA---ACTTCGAATAGGTCATCTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTGT--T-GAAG--AGTCAAATATAACTTTTTTGGAA-GCTAAGAGGATTTGTCCT-AAGCA-------------GCTTGGTTATTTCGA---------------------------------------------------AT
droEle1	AAATACG---------GGC-----T---GACTCGGAAAAGGTCATTTCCTCCAAGCGAGCAAAGAAGTAGTATTTGTTTGT-TCTGAAA--AGTCAAATATAACTTTTTTGAAA-GCTACGAGGATTTGTCCCAAATCA-------------CGTGCATTGTTTTTGAGCGAAAGACCAGGACGGATGGAAAATCAGGAGCAAAACCGTGAGAAACAAGT
droRho1	AACG------------AGC-----T---TTCTCGAAATAGGTCATCTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTGT-TCTGAAA--AGTCAAATATAACTTTTTTGAAA-GCTACGAGGATTTGTTCC-ATTAACAATAAAAGCA--GTGAAAG-CCAT------------------------------------------------------AG
droFic1	AGTGCTAAGG-ATACGCAATTA--AA---AACAAAAAAGGAGCATTTCCCCTAAGCGAGCAAAGAAGTAGTATTTGTTCGT-GCTGAAA--ACTCAAATATAACTTTTTTGAAA-GCTAAAAGGATTTGTTTGCAATCA-------------GCTGC-----AT------------------------------------------------------TT
droKik1	ATAGCCGGGAC-----ATC-----T----CCGCCAAGCTGGTCTTTTCCTCTAAGCGAGCAAAGAAGTGGTATTTGTTGCA-CTTGAAATTATTCAAATATAACTTTTTTGGAA-GCTAGAAGGAGTTGCCCACAAGCA-----CACTTTAATCCCAAT-ATTTCTC---------------------------------------------------AG
droAna3	GGTTGCA---------AT--CC--T---GGCTTGGGGTAGGTCATCTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTGC-TTTTAAT--GGTCAAATATAACTTTTTTGGAA-GCTACGAGGAGTTGTCC----------------------------------------------------------------------------------------
droWil1	ATTCA------------------------TTTTTCCAATGGCCATATCCTCTAAGCGAGCAAGAAAGTGGTATTTGTCTTT--G--GAA--ATTCAAATATAACTTTTTTGGAA-GCTACAAGGCAATGTCCACAATCA-------------AC------------------------------------------------------------------
droVir3	ATCCG-A----ATATGAGATTTACC---AATTATTAACTGGGCATTCCCTCTGAGCGAGCAGAGAAGTATTATTTGCGTGAATC--TGA--TATCAAATAAGACTTTTTTGGTTCGCTATGAGGTATTGTTCATAAATA-----AACACG--TCTGCCT-ATAT------------------------------------------------------AC
droMoj3	TCAAT--------------------------CAGACGCTGGGCATTCCCTCTGAGCGAGCAGAGAAGTATTTTT-GCGTGCATC--TGA--TATCAAATAAAACTTTTTTGGTT-GCTGCGAGGTATTGTTGA-AATGT----AAACGTT--GCTCGCT-CTCT------------------------------------------------------AC
droGri2	CA-----------------------------------AAGGACATCTTCTGTAAGCGAGCAAAAATATAATATTTGT-----------------------------------------------------------------------------------------------------------------------------------------------

20-way Secondary Structure

	dm3	droSim1	droSec1	droYak2	droEre2	droEug1	droBia1	droTak1	droEle1	droRho1	droFic1	droKik1	droAna3	droBip1	dp4	droPer1	droWil1	droVir3	droMoj3	droGri2
RNAfold Image				None											None	None
UNAFold Image				None											None	None

Small RNA-seq read pileup

Species

Alignment

droSim1

GTAA--------AC---------------TCAAT-----TGT-----------CACTGGTCGTGTCCTCCAAGCGAGCAAAGAAGTGGTATTTGCGTTT--C--CAA--GAACAAATATAACTTCTTTGAAA-GCTAGGTGGATTTGCCCA-AGTCA-------------CCTTG-GT-ATTT------------------------------------------------------AT	Size	Hit Count	Total Norm	V044 Embryo	M023 Head	M024 Male body	M025 Embryo
......................................................................AAGCGAGCAAAGAAGTGGTATT...................................................................................................................................................	22	2	15.00	0.00	0.00	15.00	0.00
....................................................................................................................TATAACTTCTTTGAAA-GCTAGG....................................................................................................	22	2	5.50	0.00	0.00	5.50	0.00
.........................................T-----------CACTGGTCGTGTCCTCC.........................................................................................................................................................................	18	2	2.00	0.00	0.50	1.50	0.00
....................................................................................................................TATAACTTCTTTGAAA-GCT.......................................................................................................	19	2	1.50	0.00	0.00	1.50	0.00
.......................................................................AGCGAGCAAAGAAGTGGTATTT..................................................................................................................................................	22	2	1.50	0.00	0.00	1.50	0.00
...................................................................................................................ATATAACTTCTTTGAAA-GCT.......................................................................................................	20	2	1.50	0.00	0.50	1.00	0.00
......................................................................AAGCGAGCAAAGAAGTGGTATTT..................................................................................................................................................	23	2	1.00	0.00	0.00	1.00	0.00
......................................................................AAGCGAGCAAAGAAGTGGTAT....................................................................................................................................................	21	2	1.00	0.00	0.00	1.00	0.00
.......................................................................AGCGAGCAAAGAAGTGGTATT...................................................................................................................................................	21	2	0.50	0.00	0.00	0.50	0.00
......................................................................AAGCGAGCAAAGAAGTGGTACT...................................................................................................................................................	22	2	0.50	0.00	0.00	0.50	0.00
....................................................................................................................TATAACTTCTTTGAAA-GC........................................................................................................	18	2	0.50	0.00	0.00	0.50	0.00
......................................................................AAGCGGGCAAAGAAGTGGTATT...................................................................................................................................................	22	2	0.50	0.00	0.00	0.50	0.00
...................................................................................................................ATATAACTTCTTTGAAA-GCTA......................................................................................................	21	2	0.50	0.00	0.00	0.50	0.00
........................................GT-----------CACTGGTCGTGTCCTCC.........................................................................................................................................................................	19	2	0.50	0.00	0.00	0.50	0.00

droSec1

AGGTAA------AC---------------TCGAT-----TGT-----------CACTGGTCGTGTCCTCCAAGCGAGCAAAGAAGTAGTATTTGCGTTT--C--CAA--GAACAAATATAACTTCTTTGAAA-GCTAGGTGGATTTGCCCA-AGTCT-------------CCTTG-GT-ATTT------------------------------------------------------AT	Size	Hit Count	Total Norm	V113 Male body	V114 Embryo	V115 Head
......................................................................AAGCGAGCAAAGAAGTAGTATT...................................................................................................................................................	22	2	622.50	595.00	5.50	22.00
......................................................................AAGCGAGCAAAGAAGTAGTATTT..................................................................................................................................................	23	2	92.50	90.50	0.50	1.50
....................................................................................................................TATAACTTCTTTGAAA-GCTAGG....................................................................................................	22	2	50.50	43.00	5.00	2.50
......................................................................AAGCGAGCAAAGAAGTAGTAT....................................................................................................................................................	21	2	17.00	16.50	0.00	0.50
.......................................................................AGCGAGCAAAGAAGTAGTATTT..................................................................................................................................................	22	2	9.00	8.50	0.50	0.00
......................................................................AAGCGAGCAAAGAAGTAGCATT...................................................................................................................................................	22	2	7.00	7.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGAATT...................................................................................................................................................	22	2	6.00	6.00	0.00	0.00
....................................................................................................................TATAACTTCTTTGAAA-GCTAG.....................................................................................................	21	2	3.00	3.00	0.00	0.00
......................................................................AACCGAGCAAAGAAGTAGTATT...................................................................................................................................................	22	2	3.00	3.00	0.00	0.00
...............................................................................................................................................TTTGCCCx-AGTGT-------------CCTTG-GT-............................................................	19	1	5.00	3.00	2.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGGATT...................................................................................................................................................	22	2	2.50	2.50	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGTA.....................................................................................................................................................	20	2	2.50	2.50	0.00	0.00
......................................................................AAGCGAGCAAAGAAGCAGTATT...................................................................................................................................................	22	2	2.00	2.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGGAGTATT...................................................................................................................................................	22	2	1.50	1.50	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTGGTATT...................................................................................................................................................	22	2	1.50	1.50	0.00	0.00
...............................................................................................................................................TTTGCCCx-AGTGT-------------CCTTG-G..............................................................	18	1	3.00	0.00	0.00	3.00
......................................................................AAGCGAGCAAAGCAGTAGTATT...................................................................................................................................................	22	2	1.50	1.50	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGT......................................................................................................................................................	19	2	1.00	1.00	0.00	0.00
......................................................................AAGCGAGCAACGAAGTAGTATT...................................................................................................................................................	22	2	1.00	1.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTCGTATT...................................................................................................................................................	22	2	1.00	1.00	0.00	0.00
..............................................................................................................................................ATTTGCCCx-AGTGT-------------CCTTG-...............................................................	18	1	2.00	0.00	2.00	0.00
......................................................................AAGCGAGCAAAGAATTAGTATT...................................................................................................................................................	22	2	1.00	1.00	0.00	0.00
................................................................................................................................................TTGCCCx-AGTGT-------------CCTTG-GT-............................................................	18	1	2.00	0.00	1.00	1.00
....................................................................................................................TATAACTTCTTTGAAA-GCTAGGT...................................................................................................	23	2	1.00	0.50	0.50	0.00
......................................................................AAGCGAGCAAAGAAGTAGGATTT..................................................................................................................................................	23	2	1.00	1.00	0.00	0.00
......................................................................AATCGAGCAAAGAAGTAGTATT...................................................................................................................................................	22	2	1.00	1.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGTATA...................................................................................................................................................	22	2	1.00	1.00	0.00	0.00
......................................................................AAGCGAGAAAAGAAGTAGTATT...................................................................................................................................................	22	2	1.00	1.00	0.00	0.00
.........................................T-----------CACTGGTCGTGTCCTCC.........................................................................................................................................................................	18	2	1.00	0.00	0.50	0.50
......................................................................AAGCGAGCAAAGAAGTAGCATTT..................................................................................................................................................	23	2	1.00	1.00	0.00	0.00
.......................................................................AGCGAGCAAAGAAGTAGTATT...................................................................................................................................................	21	2	1.00	0.50	0.00	0.50
......................................................................AAGCGAGCAAAGAAGTACTATT...................................................................................................................................................	22	2	1.00	1.00	0.00	0.00
......................................................................AAGCGAGCACAGAAGTAGTATTT..................................................................................................................................................	23	2	0.50	0.50	0.00	0.00
.......................................................................AGCGCGCAAAGAAGTAGTATTT..................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
......................................................................AAGCGAGCAAAGAAATAGTATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTAATATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
......................................................................AAGCGAGTAAAGAAGTAGTATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
......................................................................AAGCTAGCAAAGAAGTAGTATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
....................................................................................................................TATAACTACTTTGAAA-GCTAGG....................................................................................................	22	2	0.50	0.50	0.00	0.00
.......................................................................AGCGAGCAAAGAAGTAGTATTTG.................................................................................................................................................	23	2	0.50	0.50	0.00	0.00
......................................................................GAGCGAGCAAAGAAGTAGTATTT..................................................................................................................................................	23	2	0.50	0.00	0.00	0.50
......................................................................AAGCGAGCAAAGAAGTTGTATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
......................................................................AAGCGAGCAAAGCAGTAGCATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
......................................................................AAGCGAGCAAAGACGTAGTATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
...................................................................................................................CTATAACTTCTTTGAAA-GCTAGG....................................................................................................	23	2	0.50	0.00	0.00	0.50
......................................................................AAGCGAGCAAAGAAGTAGTATTA..................................................................................................................................................	23	2	0.50	0.00	0.00	0.50
......................................................................AAGTGAGCAAAGAAGTAGTATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTATTATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
.............................................................................................................................................GATTTGCCCx-AGTGT-------------CCTT.................................................................	18	2	0.50	0.00	0.50	0.00
......................................................................AAGCGAGCAAACAAGTAGTATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
....................................................................................................................TATAACxTCTTTGAAA-GCTAGG....................................................................................................	21	2	0.50	0.50	0.00	0.00
...................................................................................................................ATATAACTTCTTTGAAA-GCTAGG....................................................................................................	23	2	0.50	0.50	0.00	0.00
......................................................................AAGCGAGCAAGGAAGTAGTATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
....................................................................................................................TATAACTTCTTTGAAA-GTTAGG....................................................................................................	22	2	0.50	0.00	0.00	0.50
......................................................................AAGTGAGCAAAGAAGTAGTATTT..................................................................................................................................................	23	2	0.50	0.00	0.50	0.00
.......................................................................AGCGACCAAAGAAGTAGTATTT..................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
....................................................................................................................TACAACTTCTTTGAAA-GCTAGG....................................................................................................	22	2	0.50	0.50	0.00	0.00
....................................................................................................................TATAACTTCTTTGAAA-GCAAGG....................................................................................................	22	2	0.50	0.50	0.00	0.00
......................................................................AAGCGAGCAAAGAGGTAGTATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
....................................................................................................................TATAACTTCTTTGAAA-TCTAGG....................................................................................................	22	2	0.50	0.50	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGTACT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
....................................................................................................................TATAACTTCTTTGAAA-GCTAGA....................................................................................................	22	2	0.50	0.50	0.00	0.00
......................................................................AAGCGAGCxAAGAAGTAGTATT...................................................................................................................................................	21	2	0.50	0.50	0.00	0.00
....................................................................................................................TATAACTTCTTTGAAA-GCCAGG....................................................................................................	22	2	0.50	0.50	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTCGTATTT..................................................................................................................................................	23	2	0.50	0.50	0.00	0.00
......................................................................AAGCGCGCAAAGAAGTAGTATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
......................................................................CAGCGAGCAAAGAAGTAGTATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
....................................................................................................................TATAACTTCTTTGxGA-GCTAGG....................................................................................................	21	2	0.50	0.50	0.00	0.00
.....................................................................CAAGCGAGCAAAGAAGTAGTATT...................................................................................................................................................	23	2	0.50	0.50	0.00	0.00
......................................................................AAGCGAGGAAAGAAGTAGTATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
......................................................................AAGCGAGCAAAGGAGTAGTATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
..........................................................................GAGCAAAGAAGTAGTATT...................................................................................................................................................	18	2	0.50	0.50	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGAATTT..................................................................................................................................................	23	2	0.50	0.50	0.00	0.00
........................................GT-----------CACTGGTCGTGTCCTCC.........................................................................................................................................................................	19	2	0.50	0.00	0.50	0.00
......................................................................AAGCGAGCAAAGAAGTCGCATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGTATTTT.................................................................................................................................................	24	2	0.50	0.50	0.00	0.00
......................................................................AAGCCAGCAAAGAAGTAGTATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
......................................................................AAGCGACCAAAGAAGTAGTATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
.....................................................................CAAGCGAGCAAAGAAGTAGTAT....................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
...................................................................................................................ATATAACTTCTTTGAAA-GCTA......................................................................................................	21	2	0.50	0.00	0.50	0.00
......................................................................AAGCGAGCCAAGAAGTAGTATTT..................................................................................................................................................	23	2	0.50	0.50	0.00	0.00
......................................................................AAGCGAGCGAAGAAGTAGTATTT..................................................................................................................................................	23	2	0.50	0.50	0.00	0.00
......................................................................ACGCGAGCAAAGAAGTAGTATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
......................................................................AAACGAGCAAAGAAGTAGTATTT..................................................................................................................................................	23	2	0.50	0.50	0.00	0.00
......................................................................AAGGGAGCAAAGAAGTAGTATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
.......................................................................AGCGAGCAAAGAAGCAGTATTT..................................................................................................................................................	22	2	0.50	0.50	0.00	0.00
....................................................................................................................TATAACTTCTTTGAAA-CCTAGG....................................................................................................	22	2	0.50	0.50	0.00	0.00
......................................................................AGGCGAGCAAAGAAGTAGTATT...................................................................................................................................................	22	2	0.50	0.50	0.00	0.00

droYak2

-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Size

Hit Count

Total Norm

V046
Embryo

V052
Head

V120
Male body

V058
Head

M026
Head

droEre2

GAAAACAGAACCAC---------------TAACT-----CAG-----------CACTGGGCATGTCCTCCAAGCGAGCAAAGAAGTAGTATTTGTGTTC--C--AAA--AGTCAAATATAACTTTTTTGGAA-GCTACGGGGATTTGCCCA-AGCCA-------------ACTTG-CT-G-----------------------------------------------------------	Size	Hit Count	Total Norm	V040 Embryo	V060 Head	V047 Embryo	V108 Head
......................................................................AAGCGAGCAAAGAAGTAGTATT...................................................................................................................................................	22	1	326.00	0.00	7.00	0.00	319.00
......................................................................AAGCGAGCAAAGAAGTAGTATTT..................................................................................................................................................	23	1	154.00	0.00	11.00	0.00	143.00
...................................................................................................................................A-GGTACGGGGATCTGCCC.........................................................................................	18	1	27.00	3.00	1.00	0.00	23.00
....................................................................................................................TATAACTTTTTTGGAA-GCTACG....................................................................................................	22	1	17.00	0.00	5.00	0.00	12.00
......................................................................AAGCTAGCAAAGAAGTAGTATT...................................................................................................................................................	22	1	8.00	0.00	0.00	0.00	8.00
......................................................................AAGCGAGCAAAGAATTAGTATT...................................................................................................................................................	22	1	3.00	0.00	0.00	0.00	3.00
......................................................................AAGCGAGCAAATAAGTAGTATT...................................................................................................................................................	22	1	3.00	0.00	0.00	0.00	3.00
......................................................................AAGCGATCAAAGAAGTAGTATT...................................................................................................................................................	22	1	3.00	0.00	0.00	0.00	3.00
......................................................................AAGAGAGCAAAGAAGTAGTATTT..................................................................................................................................................	23	1	3.00	0.00	0.00	0.00	3.00
......................................................................AAGCGAGCAAAGAAGCAGTATT...................................................................................................................................................	22	1	3.00	0.00	0.00	0.00	3.00
......................................................................AATCGAGCAAAGAAGTAGTATTT..................................................................................................................................................	23	1	2.00	0.00	0.00	0.00	2.00
......................................................................AAGCGAGCAAAGAAGCAGTATTT..................................................................................................................................................	23	1	2.00	0.00	0.00	0.00	2.00
......................................................................AAGCGAGCAAAGAAGTATTATT...................................................................................................................................................	22	1	2.00	0.00	0.00	0.00	2.00
......................................................................TAGCGAGCAAAGAAGTAGTATT...................................................................................................................................................	22	1	2.00	0.00	0.00	0.00	2.00
......................................................................AGGCGAGCAAAGAAGTAGTATT...................................................................................................................................................	22	1	2.00	0.00	0.00	0.00	2.00
...................................................................................................................ATATAACTTTTTTGGAA-GCTACG....................................................................................................	23	1	2.00	0.00	0.00	0.00	2.00
......................................................................AATCGAGCAAAGAAGTAGTATTG..................................................................................................................................................	23	1	2.00	0.00	0.00	0.00	2.00
......................................................................AAACGAGCAAAGAAGTAGTATT...................................................................................................................................................	22	1	2.00	0.00	0.00	0.00	2.00
......................................................................AAGCGAGCAAAGAAGTAGTATTTT.................................................................................................................................................	24	1	2.00	0.00	0.00	0.00	2.00
...............................................................................AAGAAxTACTATTTGTGTT.............................................................................................................................................	18	2	1.00	0.50	0.00	0.00	0.50
......................................................................AATCGAGCAAAGAAGTAGTATT...................................................................................................................................................	22	1	2.00	0.00	0.00	0.00	2.00
......................................................................AAGCGAGCAAAGAAGTCGTATTT..................................................................................................................................................	23	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGAGCAAAGxAGTAGTATT...................................................................................................................................................	21	1	1.00	0.00	0.00	0.00	1.00
......................................................................CAGCGAGCAAAGAAGTAGTATT...................................................................................................................................................	22	1	1.00	0.00	1.00	0.00	0.00
......................................................................AAGCGAGCGAAGAAGTAGTATT...................................................................................................................................................	22	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGAGCAAAGAAGTAGTACTT..................................................................................................................................................	23	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGAGCAAAGAAGTAGCATTT..................................................................................................................................................	23	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGGGCAAAGAAGTAGTATT...................................................................................................................................................	22	1	1.00	0.00	1.00	0.00	0.00
......................................................................AAGCGAGCAAAGAGGTAGTATT...................................................................................................................................................	22	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGAGCAAAGAAGTAGTGT....................................................................................................................................................	21	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGAGCAAAGAAGTAGTATTC..................................................................................................................................................	23	1	1.00	0.00	0.00	0.00	1.00
......................................................................AACCGAGCAAAGAAGTAGTATTT..................................................................................................................................................	23	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGAGCAAAGCAGTAGTATTT..................................................................................................................................................	23	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGAGCAAAGAAGTATTATTC..................................................................................................................................................	23	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGAGCAAAGAAATAGTATT...................................................................................................................................................	22	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGAGCAAAGGAGTAGTATT...................................................................................................................................................	22	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGAGCAAAGACGTAGTATT...................................................................................................................................................	22	1	1.00	0.00	0.00	0.00	1.00
.........................................................................CGAGCAAAGAAGTAGTATT...................................................................................................................................................	19	1	1.00	0.00	0.00	0.00	1.00
......................................................................CAGCGAGCAAAGAAGTAGTATTT..................................................................................................................................................	23	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGACCAAAGAAGTAGTATT...................................................................................................................................................	22	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGAGCAAAGAAGTAGTATTA..................................................................................................................................................	23	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGAGCAAATAAGTAGTATTT..................................................................................................................................................	23	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGCGCAAAGAAGTAGTATT...................................................................................................................................................	22	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCTAGCAAAGAAGTAGTATTT..................................................................................................................................................	23	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGAGAAAAGAAGTAGTATT...................................................................................................................................................	22	1	1.00	0.00	0.00	0.00	1.00
....................................................................................................................TATAACxTTTTTGGAA-GCTACG....................................................................................................	21	1	1.00	0.00	1.00	0.00	0.00
......................................................................AAGCTAGCAAATAAGTAGTATT...................................................................................................................................................	22	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGAGCAAAGAAGTATTATTT..................................................................................................................................................	23	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGATCAAAGAAGTAGTATTT..................................................................................................................................................	23	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGAGCAAAGAAGTGGTATTT..................................................................................................................................................	23	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGAGCACAGAAGTAGTATTT..................................................................................................................................................	23	1	1.00	0.00	1.00	0.00	0.00
......................................................................AAGCGAGCAAAGGAGTAGTATTT..................................................................................................................................................	23	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGAGCAAAGAAGTAGTAT....................................................................................................................................................	21	1	1.00	0.00	0.00	0.00	1.00
......................................................................GAGCGAGCAAAGAAGTAGTATT...................................................................................................................................................	22	1	1.00	0.00	0.00	0.00	1.00
.....................................................................CAAGCGAGCAAAGAAGTAGTATTT..................................................................................................................................................	24	1	1.00	0.00	0.00	0.00	1.00

droEug1

AAACATAAT--AAT-----TAAGA-----TTAAG-----AAT-----------AATTGGATATGTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTAT------TA--AGTCGAATATAACTTTTTTGGAA-GCTAAGCGGATGTATCTAAACCCA-------------GCTTG-AT-GTTT------------------------------------------------------TT

Size

Hit Count

Total Norm

droBia1

AAGGCAATACAAAT----TTAG--------AATT-----TAG-----------AAAAGGTCATCTCCTCCAAGCGAGCAAAGAAGTAGTATTTGTTTGT--T--AAA--AGTCAAATATAACTTTTTTGGAA-GCTAAGAGGATTTATCCT-AACCA-------------GCTTG----------------------------------------------------------------

Size

Hit Count

Total Norm

droTak1

ATTTGGA------------------------ACT-----TCG-----------AATAGGTCATCTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTGT--T-GAAG--AGTCAAATATAACTTTTTTGGAA-GCTAAGAGGATTTGTCCT-AAGCA-------------GCTTG-GTTATTTCGA---------------------------------------------------AT

Size

Hit Count

Total Norm

droEle1

AAATACGG----GC---------------TGACT-----CGG-----------AAAAGGTCATTTCCTCCAAGCGAGCAAAGAAGTAGTATTTGTTTGT-TCTGAAA--AGTCAAATATAACTTTTTTGAAA-GCTACGAGGATTTGTCCCAAATCA-------------CG-TGCATTGTTTTTGAGCGAAAGACCAGGACGGATGGAAAATCAGGAGCAAAACCGTGAGAAACAAGT

Size

Hit Count

Total Norm

droRho1

AACGA-------GC---------------TTTCT-----CGA-----------AATAGGTCATCTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTGT-TCTGAAA--AGTCAAATATAACTTTTTTGAAA-GCTACGAGGATTTGTTCC-ATTAACAATAAAAGCA--GTGAA-AG-CCAT------------------------------------------------------AG

Size

Hit Count

Total Norm

droFic1

AGTGCTAAGGATACGCAATTAA--------AAAC-----AAA-----------AAAGGAGCATTTCCCCTAAGCGAGCAAAGAAGTAGTATTTGTTCGT-GCTGAAA--ACTCAAATATAACTTTTTTGAAA-GCTAAAAGGATTTGTTTGCAATCA-------------GCT------GCAT------------------------------------------------------TT

Size

Hit Count

Total Norm

droKik1

ATAGCCGGGACATC---------------TC-CG-----CCA-----------AGCTGGTCTTTTCCTCTAAGCGAGCAAAGAAGTGGTATTTGTTGCA-CTTGAAATTATTCAAATATAACTTTTTTGGAA-GCTAGAAGGAGTTGCCCACAAGCA-----CACTTTAATCCCA-AT-ATTTCTC---------------------------------------------------AG

Size

Hit Count

Total Norm

droAna3

GGTTGCAA----T--------------CCTGGCT-----TGG-----------GGTAGGTCATCTCCTCTAAGCGAGCAAAGAAGTAGTATTTGTTTGC-TTTTAAT--GGTCAAATATAACTTTTTTGGAA-GCTACGAGGAGTTGTCC-----------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	V105 Male body	V106 Head	V055 Unknown	V039 Embryo
......................................................................AAGCGAGCAAAGAAGTAGTATT...................................................................................................................................................	22	1	350.00	294.00	37.00	19.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGTATTT..................................................................................................................................................	23	1	113.00	91.00	17.00	5.00	0.00
....................................................................................................................TATAACTTTTTTGGAA-GCTACG....................................................................................................	22	1	79.00	74.00	0.00	5.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGTAT....................................................................................................................................................	21	1	54.00	51.00	1.00	2.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGTATTTT.................................................................................................................................................	24	1	11.00	7.00	4.00	0.00	0.00
.......................................................................AGCGAGCAAAGAAGTAGTATTT..................................................................................................................................................	22	1	9.00	5.00	4.00	0.00	0.00
....................................................................................................................TATAACTTTTTTGGAA-GCTACGT...................................................................................................	23	1	8.00	7.00	0.00	1.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGTAAT...................................................................................................................................................	22	1	4.00	4.00	0.00	0.00	0.00
.......................................................................AGCGAGCAAAGAAGTAGTATT...................................................................................................................................................	21	1	4.00	4.00	0.00	0.00	0.00
......................................................................AATCGAGCAAAGAAGTAGTATT...................................................................................................................................................	22	1	3.00	0.00	3.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGTAGTT..................................................................................................................................................	23	1	3.00	3.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGTA.....................................................................................................................................................	20	1	3.00	2.00	0.00	1.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGTAGT...................................................................................................................................................	22	1	3.00	3.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTGGTATT...................................................................................................................................................	22	1	3.00	0.00	2.00	1.00	0.00
......................................................................AAGCGAGCAAAGAAGCAGTATT...................................................................................................................................................	22	1	3.00	3.00	0.00	0.00	0.00
....................................................................................................................TATAACTTTTTTGGAA-GCTAC.....................................................................................................	21	1	2.00	2.00	0.00	0.00	0.00
...................................................................................................................ATATAACTTTTTTGGAA-GCTAC.....................................................................................................	22	1	2.00	2.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGTATTTTT................................................................................................................................................	25	1	2.00	1.00	1.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGTACT...................................................................................................................................................	22	1	2.00	2.00	0.00	0.00	0.00
......................................................................AAGCGATCAAAGAAGTAGTATT...................................................................................................................................................	22	1	2.00	2.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGGAGTATT...................................................................................................................................................	22	1	2.00	1.00	0.00	1.00	0.00
....................................................................................................................TATAACTTTTTTTGAA-GCTACG....................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCxAAGAAGTAGTATTTT.................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGTAG....................................................................................................................................................	21	1	1.00	0.00	1.00	0.00	0.00
....................................................................................................................TATAACTTTTTTAGAA-GCTACG....................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
....................................................................................................................TATAACTTTTTTGGAA-GCTATG....................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AATCGAGCAAAGAAGTAGTATTT..................................................................................................................................................	23	1	1.00	0.00	1.00	0.00	0.00
......................................................................AAGAGAGAAAAGAAGTAGTATTT..................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AATCGATCAAAGAAGTAGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
....................................................................................................................TATAACTTTTTTGGAA-GCTACGA...................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAATGAAGTTGTATT...................................................................................................................................................	22	1	1.00	0.00	0.00	1.00	0.00
.............................................................................................................GGTCGAAGATAACTTTTTT...............................................................................................................	19	1	1.00	0.00	1.00	0.00	0.00
......................................................................AAGCGAGCAAAAAAGTAGTATTT..................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGGGCAAAGAAGTAGTATT...................................................................................................................................................	22	1	1.00	0.00	1.00	0.00	0.00
......................................................................AAGCGATCAAAGAAGTAGTTT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGTAAAGAAGTAGTAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
..........................................................................................................T--GGTCGAAGATAACTTTTT................................................................................................................	19	1	1.00	0.00	1.00	0.00	0.00
......................................................................GAGCGAGCAAAGAAGTAGTAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGAAGTATTT..................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTATTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGAAAAGAGGTAGTATT...................................................................................................................................................	22	1	1.00	0.00	0.00	1.00	0.00
......................................................................AAGCGAGCAAACAAGTAGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGAAGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAACGAAGTAGTATTT..................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGCAGTAGTATTT..................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCTAGCAAAGAAGTAGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGATCAAAGAAGTAGTAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAATGAAGTAGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
....................................................................................................................TATAACTTTTTTGGAA-GCTA......................................................................................................	20	1	1.00	1.00	0.00	0.00	0.00
.......................................................................AGCGAGCAAAGAAGTAGTGTTT..................................................................................................................................................	22	1	1.00	0.00	0.00	1.00	0.00
....................................................................................................................TATAACTTTTATGGAA-TCTACG....................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGAGAGCAAAGAAGTAGTATTT..................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
..................................................................................................................AATATAACTTTTTTGGAA-TCT.......................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAATGAAGTAGTAA....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
........................................................................GCGAGCAAAGACGTAGTATTT..................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
......................................................................AGGCGAGCAAAGAAGTAGTATTTT.................................................................................................................................................	24	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGATGTAGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCTAGCAAAGAAGTAGTATTT..................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCGAAGAAGTAGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
.......................................................................AGCGAGCAAAGCAGTAGTATTT..................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGACGTATTATTT..................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGTATTTC.................................................................................................................................................	24	1	1.00	0.00	1.00	0.00	0.00
...................................................................................................................ATATAACTTTTTTGGAA-GCTA......................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
....................................................................................................................TATAACTTTTTTGCAA-GCTACG....................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTACTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGCAGTAGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGTGTT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................ACGCGAGCAAAGAAGTAGTATTT..................................................................................................................................................	23	1	1.00	0.00	1.00	0.00	0.00
......................................................................AATCGAGCAAAGAAGTAGTAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGCAGTAGTAGT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGTAGTAGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AGGCTAGCAAAGAAGTAGTATTT..................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGAAGTAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
........................................................................................TAGTTGTTTGC-TTTTCAT--G.................................................................................................................................	19	1	1.00	0.00	0.00	0.00	1.00
......................................................................AAGCGAACAAAGAAGTAGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGTACTTT.................................................................................................................................................	24	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGxAGTAGTATT...................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
.....................................................................CAAGCGAGCAAAGAAGTAGTATT...................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGTATTC..................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAAGAAGTAGTT.....................................................................................................................................................	20	1	1.00	1.00	0.00	0.00	0.00
....................................................................................................................TATAACTTTCTTGGAA-GCTACG....................................................................................................	22	1	1.00	0.00	0.00	1.00	0.00
........................................................................GCGAGCAAAGAAGTAGTATTG..................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
......................................................................AGGCGAGCAAAGAAGTAGTATTTTT................................................................................................................................................	25	1	1.00	1.00	0.00	0.00	0.00

droBip1

ATCCCGAACCAATCGCGGTTGC--AATCC-----------TTGGGTGGATTGGGGTAGGTCATCTCCTCTAAGCGAGCAAAGAAGGCGTAT----------------------------------------------------------------------------------------------------------------------------------------------------

Size

Hit Count

Total Norm

dp4

Size

Hit Count

Total Norm

V112
Male body

V043
Embryo

V051
Head

M022
Male body

droPer1

Size

Hit Count

Total Norm

V111
Male body

V042
Embryo

V050
Head

V057
Head

M021
Embryo

droWil1

ATTCAT--------------------------TT-----TTC-----------CAATGGCCATATCCTCTAAGCGAGCAAGAAAGTGGTATTTGTCTTT--G--GAA--ATTCAAATATAACTTTTTTGGAA-GCTACAAGGCAATGTCCACAATCA-------------AC-------------------------------------------------------------------	Size	Hit Count	Total Norm	V117 Male body	V118 Embryo	V119 Head	M020 Head
......................................................................AAGCGAGCAAGAAAGTGGTATT...................................................................................................................................................	22	1	661.00	649.00	7.00	4.00	1.00
......................................................................AAGCGAGCAAGAAAGTGGTATTT..................................................................................................................................................	23	1	149.00	144.00	3.00	2.00	0.00
......................................................................AAGCGAGCAAGAAAGTGGTAT....................................................................................................................................................	21	1	51.00	50.00	0.00	1.00	0.00
......................................................................AAGCGAGCAAGAAAGTGGTATTTC.................................................................................................................................................	24	1	5.00	5.00	0.00	0.00	0.00
....................................................................................................................TATAACTTTTTTGGAA-GCTACA....................................................................................................	22	1	5.00	4.00	1.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGAGGTATT...................................................................................................................................................	22	1	5.00	5.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGTGGTA.....................................................................................................................................................	20	1	5.00	5.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGTGGAATT...................................................................................................................................................	22	1	4.00	4.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGGGGTATT...................................................................................................................................................	22	1	3.00	3.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGTGGGATT...................................................................................................................................................	22	1	3.00	3.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGTxGTATT...................................................................................................................................................	21	1	3.00	3.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGCGGTATT...................................................................................................................................................	22	1	2.00	2.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGCGGTATTT..................................................................................................................................................	23	1	2.00	2.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGTGGTATTA..................................................................................................................................................	23	1	2.00	2.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGTGGTACT...................................................................................................................................................	22	1	2.00	2.00	0.00	0.00	0.00
.......................................................................AGCGAGCAAGAAAGTGGTATTT..................................................................................................................................................	22	1	2.00	2.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGTGGTATTTCC................................................................................................................................................	25	1	2.00	2.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGTGGTGTT...................................................................................................................................................	22	1	2.00	2.00	0.00	0.00	0.00
......................................................................AACCGAGCAAGAAAGTGGTATT...................................................................................................................................................	22	1	2.00	1.00	1.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGTGGTATC...................................................................................................................................................	22	1	2.00	2.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGTGGCATT...................................................................................................................................................	22	1	2.00	2.00	0.00	0.00	0.00
......................................................................AAACGAGCAAGAAAGTGGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
....................................................................................................................TATAGCTTTTTTGGAA-GCTA......................................................................................................	20	1	1.00	0.00	0.00	1.00	0.00
.......................................................................AGCGAGCAAGAAAGTGGTATTTA.................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGTCGTATTT..................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGTGGTATTAA.................................................................................................................................................	24	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGCAGTATTT..................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAATGTGGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGACCAAGAAAGTGGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGTGAGCAAGAAAGTGGTATTT..................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGTGGTATTTT.................................................................................................................................................	24	1	1.00	0.00	1.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGTGGTATCT..................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGTGTTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
.............................................................................GAAAAAAGTGGTATTTGT................................................................................................................................................	18	1	1.00	0.00	1.00	0.00	0.00
......................................................................AAGCGAGCAAGACAGTGGTATTT..................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGTGAGCAAGAAAGTGGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAxxAAAGTGGTATT...................................................................................................................................................	20	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGATCAAGAAAGTGGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAGAGTGGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGTGGTCTT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGAGAGCAAGAAAGTGGTATTT..................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGTCGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
....................................................................................GTGGTATTTGGCTGT--G--GA.....................................................................................................................................	18	1	1.00	0.00	1.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGTGATATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAATAAAGTGGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAATTGGTATTT..................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGxAAGTGGTATT...................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
.....................................................................CAAGCGAGCAAGAAAGTGGTATT...................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................GAGCGAGCAAGAAAGTGGTATT...................................................................................................................................................	22	1	1.00	0.00	0.00	1.00	0.00
.............................................................................................................ATTCAAATAAAACTGTTT................................................................................................................	18	3	0.33	0.00	0.00	0.00	0.33
......................................................................AAGCGAGCAAGTAAGTGGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCGAGAAAGTGGTATTT..................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
......................................................................AAGCGAGCAAGAAAGTGGTATTC..................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00
............................................................................................TGACTTT--G--GAA--ATTCAAA...........................................................................................................................	18	2	0.50	0.00	0.50	0.00	0.00
......................................................................AAGCGAGCAAGCAAGTGGTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
..........................................................................................................................TTTTTTGGAA-GCTAGGAG..................................................................................................	18	1	1.00	0.00	1.00	0.00	0.00

droVir3

ATCCGAATATGAGA---------------TTTACCAATTATT-----------AACTGGGCATTCCCTCTGAGCGAGCAGAGAAGTATTATTTGCGTGAATC--TGA--TATCAAATAAGACTTTTTTGGTTCGCTATGAGGTATTGTTCATAAATA-----AACACG--TCTGC-CT-ATAT------------------------------------------------------AC	Size	Hit Count	Total Norm	V116 Male body	V053 Unknown	V047 Embryo	M027 Male body	M028 Head
......................................................................GAGCGAGCAGAGAAGTATTAT....................................................................................................................................................	21	1	347.00	340.00	0.00	1.00	6.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTATC...................................................................................................................................................	22	1	164.00	162.00	0.00	0.00	2.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTA.....................................................................................................................................................	20	1	77.00	76.00	0.00	0.00	1.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTC.....................................................................................................................................................	20	1	64.00	63.00	0.00	0.00	1.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTATCC..................................................................................................................................................	23	1	42.00	41.00	0.00	0.00	1.00	0.00
......................................................................GAGCGAGCAGAGAAGTATT......................................................................................................................................................	19	1	37.00	36.00	1.00	0.00	0.00	0.00
...................................................................................................................ATAAGACTTTTTTGGTTCGCTATG....................................................................................................	24	1	28.00	13.00	0.00	0.00	15.00	0.00
....................................................................................................................TAAGACTTTTTTGGTTCGCTATG....................................................................................................	23	1	25.00	20.00	0.00	0.00	5.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTATTT..................................................................................................................................................	23	1	24.00	23.00	0.00	0.00	0.00	1.00
......................................................................GAGCGAGCAGAGAAGTATTATT...................................................................................................................................................	22	1	19.00	18.00	1.00	0.00	0.00	0.00
....................................................................................................................TAAGACTTTTTTGGTTCGCTAT.....................................................................................................	22	1	18.00	18.00	0.00	0.00	0.00	0.00
...................................................................................................................ATAAGACTTTTTTGGTTCGCT.......................................................................................................	21	1	16.00	9.00	0.00	0.00	7.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTATTC..................................................................................................................................................	23	1	15.00	14.00	0.00	0.00	1.00	0.00
...................................................................................................................ATAAGACTTTTTTGGTTCGCTC......................................................................................................	22	1	11.00	3.00	0.00	0.00	8.00	0.00
...................................................................................................................ATAAGACTTTTTTGGTTCGCTAT.....................................................................................................	23	1	10.00	10.00	0.00	0.00	0.00	0.00
...................................................................................................................ATAAGACTTTTTTGGxTCGTTA......................................................................................................	21	1	9.00	5.00	0.00	0.00	4.00	0.00
...................................................................................................................ATAAGACTTTTTTGGTTCGCTA......................................................................................................	22	1	7.00	7.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTCC....................................................................................................................................................	21	1	6.00	6.00	0.00	0.00	0.00	0.00
....................................................................................................................TAAGACTTTTTTGGTTCGCTA......................................................................................................	21	1	3.00	3.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTATTTC.................................................................................................................................................	24	1	3.00	3.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTCA....................................................................................................................................................	21	1	3.00	3.00	0.00	0.00	0.00	0.00
....................................................................................................................TAAGACTTTTTTGGTTCGCT.......................................................................................................	20	1	2.00	1.00	0.00	0.00	1.00	0.00
...................................................................................................................ATAAGACTTTTTTGGTTCGCC.......................................................................................................	21	1	2.00	0.00	0.00	0.00	2.00	0.00
......................................................................GAGCGAGCAAAGAAGTATTATC...................................................................................................................................................	22	1	2.00	2.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGCATTC.....................................................................................................................................................	20	1	2.00	2.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTAA....................................................................................................................................................	21	1	2.00	2.00	0.00	0.00	0.00	0.00
....................................................................................................................TAAGACTTTTTTGGTTCGCTATC....................................................................................................	23	1	2.00	1.00	0.00	0.00	1.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTT.....................................................................................................................................................	20	1	2.00	2.00	0.00	0.00	0.00	0.00
....................................................................................................................TAAGACTTTTTTGGTTCGCC.......................................................................................................	20	1	1.00	0.00	0.00	0.00	1.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTCT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00	0.00
...................................................................................................................ATAAGACTTTTTTGGTTCGCTT......................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGACAAGTATTA.....................................................................................................................................................	20	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTATA...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00	0.00
...................................................................................................................ATAAGACTTTATTGGTTCGCTA......................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00	0.00
.......................................................................AGCGAGCAGAGAAGTATTAT....................................................................................................................................................	20	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAATATT......................................................................................................................................................	19	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGTGAGCAGAGAAGTATTATC...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGGATTC.....................................................................................................................................................	20	1	1.00	1.00	0.00	0.00	0.00	0.00
...................................................................................................................ATAAGACTTTTTTGGTTCACT.......................................................................................................	21	1	1.00	0.00	0.00	0.00	1.00	0.00
....................................................................................................................TAAGACTTTTTTGGTTCGCTATGT...................................................................................................	24	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTGTT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00	0.00
...................................................................................................................ATAAGACTTTTTTGGTTCGCTGT.....................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00	0.00
..........................................................................GAGCAGAGAAGTATTATC...................................................................................................................................................	18	1	1.00	1.00	0.00	0.00	0.00	0.00
....................................................................................................................TAAGACTTTTTTGGTTCGCTATT....................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATCAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCAAGCAGAGAAGTATTATC...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAACAGAGAAGTATTA.....................................................................................................................................................	20	1	1.00	1.00	0.00	0.00	0.00	0.00
....................................................................................................................TAAGACTTTTTTGGCTCGCTATGT...................................................................................................	24	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCAAGCAGAGAAGTATTA.....................................................................................................................................................	20	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGGGCAGAGAAGTATTA.....................................................................................................................................................	20	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTACTAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00	0.00
....................................................................................................................TAAGACTTCTTTGGTTCGCTATG....................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTGT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTACTA.....................................................................................................................................................	20	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGAATTAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAxAAGTATTATTC..................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGCGAAGTATTA.....................................................................................................................................................	20	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GTGCGAGCAGAGAAGTATTC.....................................................................................................................................................	20	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTAATC.....................................................................................................................................................	20	1	1.00	1.00	0.00	0.00	0.00	0.00
.....................................................................CGAGCGAGCAGAGAAGTATTATC...................................................................................................................................................	23	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGATCAGAGAAGTATT......................................................................................................................................................	19	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCGGAGAAGTATTAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCCAGCAGAGAAGTATTAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGAAGAGAAGTATTC.....................................................................................................................................................	20	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................TAGCGAGCAGAGAAGTATTC.....................................................................................................................................................	20	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATAAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTACTATC...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAACTATTAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00	0.00
....................................................................................................................TAAGACTTTTTTGGxTCGTTA......................................................................................................	20	1	1.00	1.00	0.00	0.00	0.00	0.00
............................................................................................TGCGTGAATC--TGA--TATCAAA...........................................................................................................................	20	1	1.00	1.00	0.00	0.00	0.00	0.00
....................................................................................................................TAAGACTTTTTTGGTTCGCTATGTC..................................................................................................	25	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGGGAAGTATTAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGGAGTATTAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATC......................................................................................................................................................	19	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCGGAGAAGTATTATT...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00	0.00
...................................................................................................................ATAAGACTTTTTTGGATCGCT.......................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00	0.00
.........................................................................................................................CTTTTTTGGTTCGCTATG....................................................................................................	18	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GATCGAGCAGAGAAGTATTAA....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00	0.00
...................................................................................................................ATAAGACTTTTTTGGTTCGCTATC....................................................................................................	24	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGCAGTATTAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGATGTATTATC...................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00	0.00

droMoj3

TCAATC---------------------------------AGA-----------CGCTGGGCATTCCCTCTGAGCGAGCAGAGAAGTATTTTT-GCGTGCATC--TGA--TATCAAATAAAACTTTTTTGGTT-GCTGCGAGGTATTGTTGA-AATGT----AAACGTT--GCTCG-CT-CTCT------------------------------------------------------AC	Size	Hit Count	Total Norm	V110 Male body	V056 Unknown	V049 Head	V041 Embryo
......................................................................GAGCGAGCAGAGAAGTATTAT....................................................................................................................................................	21	1	128.00	125.00	3.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTA.....................................................................................................................................................	20	1	112.00	109.00	2.00	1.00	0.00
......................................................................GAGCGAGCAGAGAAGTATT......................................................................................................................................................	19	1	36.00	35.00	1.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTT.....................................................................................................................................................	20	1	13.00	13.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTAA....................................................................................................................................................	21	1	12.00	11.00	0.00	1.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTATC-..................................................................................................................................................	22	1	10.00	10.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTATT-..................................................................................................................................................	22	1	8.00	8.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTC.....................................................................................................................................................	20	1	7.00	7.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTATT-T.................................................................................................................................................	23	1	5.00	5.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTTT....................................................................................................................................................	21	1	2.00	2.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTATT-C.................................................................................................................................................	23	1	2.00	2.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGTAGTATTAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTTC....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAATTATTAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATTCT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
......................................................................GAACGAGCAGAGAAGTATTAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGAGAAGTATC......................................................................................................................................................	19	1	1.00	1.00	0.00	0.00	0.00
......................................................................GAGCTAGCAGAGAAGTATTAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
......................................................................GACCGAGCAGAGAAGTATTATT-..................................................................................................................................................	22	1	1.00	1.00	0.00	0.00	0.00
......................................................................GAGCGAxCAGAGAAGTATTAT....................................................................................................................................................	20	1	1.00	1.00	0.00	0.00	0.00
......................................................................GAGCGATCAGAGAAGTATTAT....................................................................................................................................................	21	1	1.00	1.00	0.00	0.00	0.00
......................................................................GATCGAGCAGAGAAGTATT......................................................................................................................................................	19	1	1.00	1.00	0.00	0.00	0.00
......................................................................GAGCGAGCAGATAAGTATT......................................................................................................................................................	19	1	1.00	1.00	0.00	0.00	0.00

droGri2

CA-----------------------------------------------------AAGGACATCTTCTGTAAGCGAGCAAAAATATAATATTTGT------------------------------------------------------------------------------------------------------------------------------------------------

Size

Hit Count

Total Norm

V109
Male body

Generated: 04/20/2012 at 05:16:33 EDT

dme-mir-974

chrX:19445711-19445811 +

Known_mirbase

intergenic

20-way Fast Statistical Aligner (FSA) re-alignment

Visually pleasing best hits from LASTZ and MAF

Legend

Re-alignment after removal of non-confident orthologs

20-way Secondary Structure

Small RNA-seq read pileup