ID: uc011kzu.1_intron_1_0_chr8_23291992_r.3p
GENE: ENTPD4(11)
chr8:23291942-23292191-


(1)
AGO3.ip 		(6)
B-CELL 		(15)
BREAST 		(23)
CELL-LINE 		(3)
FIBROBLAST 		(6)
HEART 		(2)
LIVER 		(2)
OTHER 		(33)
SKIN 		(1)
XRN.ip

Sense strand
AACTGCTCCATGTTGTGGGACCAGGGATGTGAAGCAAGTACATCAACCTTGAAACGCGCGCAGTTGGTTTGGGAGAGCCGGCCTGAGGAAGCCCCTGCCCCAAGGCTGCCCACAGAGGGAAGGATTGTGTGTGTGTGTGTGTGTGTGCCATCTTGACAGAGTGAGTCAGTCTGACTTGCCTTTGTGCTTGCCGTTTGTAGGTATCAGTGCTTCAAATCGGCCTGGATGTTTGAGGTGTTTCATAGGGGCT
.......................................................((...((((((((((....)))))))).)).....))....(((....)))..(((.....)))...................................................................................................................................
..................................................51.....................................................................122..............................................................................................................................
SizePerfect hitTotal NormPerfect NormSRR207110(GSM721072)
Nuclear RNA. (cell line)		SRR314796(SRX084354)
"Total RNA, fractionated (15-30nt)". (cell line)		SRR207115(GSM721077)
XRN1&2 knockdown. (XRN1/XRN2 cell line)		DRR000558(DRX000316)
"THP-1 whole cell RNA, after 3 day treatment . (cell line)		SRR553572(SRX182778)
source: Frontal Cortex. (Frontal Cortex)		SRR553573(SRX182779)
source: Cerebellum. (Cerebellum)		SRR444067(SRX128915)
Sample 24cDNABarcode: AF-PP-340: ACG CTC TTC . (cell line)		SRR342898(SRX096794)
small RNA seq of Right atrial tissue. (heart)		SRR342896(SRX096792)
small RNA seq of Right atrial tissue. (heart)		SRR033712(GSM497057)
Burkitt Lymphoma (BL510). (B cell)		TAX577746(Rovira)
total RNA. (breast)		SRR330888(SRX091726)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR342901(SRX096797)
small RNA seq of Left atrial tissue. (heart)		SRR033710(GSM497055)
GCB DLBCL (GCB385). (B cell)		SRR015364(GSM380329)
Plasma B cells (PC44). (B cell)		SRR033727(GSM497072)
HIV-positive DL (HIV412). (B cell)		DRR000559(DRX000317)
"THP-1 whole cell RNA, no treatment". (cell line)		SRR330868(SRX091706)
tissue: skin psoriatic involveddisease state:. (skin)		SRR342900(SRX096796)
small RNA seq of Right atrial tissue. (heart)		SRR444052(SRX128900)
Sample 12cDNABarcode: AF-PP-340: ACG CTC TTC . (skin)		SRR330879(SRX091717)
tissue: skin psoriatic involveddisease state:. (skin)		DRR000555(DRX000313)
"THP-1 whole cell RNA, no treatment". (cell line)		SRR039620(GSM531983)
HBV(+) Adjacent Tissue Sample 2. (liver)		SRR330922(SRX091760)
tissue: normal skindisease state: normal. (skin)		SRR330903(SRX091741)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR326279(GSM769509)
cytoplasmic fraction was isolated using PARIS. (cell line)		SRR189782TAX577740(Rovira)
total RNA. (breast)		SRR038860(GSM458543)
MM426. (cell line)		SRR330877(SRX091715)
tissue: skin psoriatic involveddisease state:. (skin)		SRR330858(SRX091696)
tissue: skin psoriatic involveddisease state:. (skin)		SRR330866(SRX091704)
tissue: skin psoriatic involveddisease state:. (skin)		TAX577745(Rovira)
total RNA. (breast)		SRR330906(SRX091744)
tissue: normal skindisease state: normal. (skin)		SRR330874(SRX091712)
tissue: skin psoriatic involveddisease state:. (skin)		SRR330915(SRX091753)
tissue: normal skindisease state: normal. (skin)		SRR330921(SRX091759)
tissue: normal skindisease state: normal. (skin)		SRR363675(GSM830252)
cell type: human foreskin fibroblasts (HFF)tr. (fibroblast)		SRR330902(SRX091740)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR363674(GSM830251)
cell type: human foreskin fibroblasts (HFF)tr. (fibroblast)		SRR330876(SRX091714)
tissue: skin psoriatic involveddisease state:. (skin)		SRR330867(SRX091705)
tissue: skin psoriatic involveddisease state:. (skin)		SRR330889(SRX091727)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR330872(SRX091710)
tissue: skin psoriatic involveddisease state:. (skin)		SRR037936(GSM510474)
293cand1. (cell line)		TAX577580(Rovira)
total RNA. (breast)		SRR107297(GSM677704)
18-30nt fraction of small RNA. (cell line)		DRR000562(DRX000320)
Isolation of RNA following immunoprecipitatio. (ago3 cell line)		SRR330892(SRX091730)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR189787SRR330863(SRX091701)
tissue: skin psoriatic involveddisease state:. (skin)		SRR330890(SRX091728)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR330891(SRX091729)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR060984(GSM569188)
Human plasma cell [09-001]. (cell line)		SRR037937(GSM510475)
293cand2. (cell line)		SRR330911(SRX091749)
tissue: normal skindisease state: normal. (skin)		SRR060982(GSM569186)
Human centrocyte [09-001]. (cell line)		SRR444048(SRX128896)
Sample 8cDNABarcode: AF-PP-333: ACG CTC TTC C. (skin)		SRR330887(SRX091725)
tissue: skin psoriatic uninvolveddisease stat. (skin)		TAX577739(Rovira)
total RNA. (breast)		SRR189779(GSM714639)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR330917(SRX091755)
tissue: normal skindisease state: normal. (skin)		SRR330896(SRX091734)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR189780(GSM714640)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR363676(GSM830253)
cell type: human foreskin fibroblasts (HFF)tr. (fibroblast)		SRR330901(SRX091739)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR191619(GSM715729)
166genomic small RNA (size selected RNA from . (breast)		TAX577744(Rovira)
total RNA. (breast)		TAX577589(Rovira)
total RNA. (breast)		SRR038863(GSM458546)
MM603. (cell line)		SRR191585(GSM715695)
196genomic small RNA (size selected RNA from . (breast)		SRR191582(GSM715692)
95genomic small RNA (size selected RNA from t. (breast)		SRR015360(GSM380325)
Plasma B cells (PC137). (B cell)		TAX577738(Rovira)
total RNA. (breast)		SRR342895(SRX096791)
small RNA seq of Left atrial tissue. (heart)		SRR191623(GSM715733)
18genomic small RNA (size selected RNA from t. (breast)		SRR342897(SRX096793)
small RNA seq of Left atrial tissue. (heart)		SRR039619(GSM531982)
HBV(+) HCC Tissue Sample 1. (liver)		SRR330920(SRX091758)
tissue: normal skindisease state: normal. (skin)		SRR330916(SRX091754)
tissue: normal skindisease state: normal. (skin)		SRR191527(GSM715637)
128genomic small RNA (size selected RNA from . (breast)		SRR039637(GSM518474)
THP1_total_sRNAs. (cell line)		TAX577743(Rovira)
total RNA. (breast)		SRR191622(GSM715732)
175genomic small RNA (size selected RNA from . (breast)		SRR029125(GSM416754)
U2OS. (cell line)		SRR207116(GSM721078)
Nuclear RNA. (cell line)		DRR000556(DRX000314)
"THP-1 whole cell RNA, after 3 day treatment . (cell line)		SRR330884(SRX091722)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR330912(SRX091750)
tissue: normal skindisease state: normal. (skin)		SRR330913(SRX091751)
tissue: normal skindisease state: normal. (skin)		DRR000557(DRX000315)
"THP-1 whole cell RNA, after 3 day treatment . (cell line)		SRR033721(GSM497066)
Ly3 cell line (Ly3). (B cell)
.....................................................................................................AAGGCTGCCCACAGAGG....................................................................................................................................17157.0057.001.00-1.003.00-1.00-3.003.00-1.002.003.00-2.00--1.003.00-1.002.00--1.001.00-2.00-2.00--1.00--1.00-1.001.00-1.001.001.001.00--1.001.001.00---1.00-----1.00--1.001.00-1.001.001.001.00--1.001.00--1.00-1.00--1.00------------
....................................................................................................CAAGGCTGCCCACAGAG.....................................................................................................................................17115.0015.00---1.00--1.00---1.001.00----1.00--------1.00----1.002.00-1.00-----------1.00---------------------------1.00-1.00------1.001.00--------
....................................................................................................CAAGGCTGCCCACAGAGG....................................................................................................................................18110.0010.00----------1.00--1.00---1.00----------2.00-1.00--------------------1.00-1.00---------------1.00----------1.00-----------
.........................................................................................................................................................................................................................CGGCCTGGATGTTTGAGG...............1819.009.009.00-------------------------------------------------------------------------------------------
.....................................................................................................AAGGCTGCCCACAGAG.....................................................................................................................................1628.508.50------2.50-0.50--------1.00-2.50-------------0.500.500.50--------------------------------------------------0.50-----
.....................................................................................................AAGGCTGCCCACAGAGGATC.................................................................................................................................2017.0057.00-7.00------------------------------------------------------------------------------------------
...................................................................................................CCAAGGCTGCCCACAGAG.....................................................................................................................................1816.006.00--1.00----------2.00--------1.00-1.00-------------------------------1.00-----------------------------------
......................................................................................................AGGCTGCCCACAGAGG....................................................................................................................................1643.753.75---0.250.750.50-0.50----------------------------0.250.25-0.250.25-----------------------------------------------0.250.250.25-
.................................................................................................................................................................................................................CTTCAAATCGGCCTGGATGTT....................2113.003.00---------3.00----------------------------------------------------------------------------------
...................................................................................................CCAAGGCTGCCCACAGAGG....................................................................................................................................1913.003.00--------------------------------------------------1.00---1.00-----------------------------1.00-------
...........................................................................................................................................................................................................................GCCTGGATGTTTGAGGTGTTTCA........2312.002.00---------------2.00----------------------------------------------------------------------------
.....................................................................................................AAGGCTGCCCACAGAGGGAAG................................................................................................................................2112.002.00--------------1.00---------------------------------------------------------------1.00-------------
.................................................................GGTTTGGGAGAGCCGGCCTGAGG..................................................................................................................................................................2312.002.00--2.00-----------------------------------------------------------------------------------------
.........................................................................................................................GGATTGTGTGTGTGTGTGTGTGGGT........................................................................................................251.000.00-----1.00--------------------------------------------------------------------------------------
.......................................................................................GAAGCCCCTGCCCCAAGGCTGCCCACAGAGG....................................................................................................................................3111.001.00-------------------------------------------------------1.00------------------------------------
.......................................................................GGAGAGCCGGCCTGAGGAAGCCCCTGCCCC.....................................................................................................................................................3011.001.00-----------------------1.00--------------------------------------------------------------------
..................................................................................................................................................................................................................TTCAAATCGGCCTGGATGTTTG..................2211.001.00--1.00-----------------------------------------------------------------------------------------
........................GGATGTGAAGCAAGTAC.................................................................................................................................................................................................................1711.001.00--------------------------------------------------------------------1.00-----------------------
...........................................................................................CCCCTGCCCCAAGGCTGCCCACAGAG.....................................................................................................................................2611.001.00------------------------------------------------------------------------1.00-------------------
.....................................................................................................AAGGCTGCCCACAGAGGGAAGG...............................................................................................................................2211.001.00--------------------1.00-----------------------------------------------------------------------
............................................................................................CCCTGCCCCAAGGCTGCCCACAGAGG....................................................................................................................................2611.001.00-----------------------1.00--------------------------------------------------------------------
.....................................................................................................AAGGCTGCCCACAGAGGGAAGGAT.............................................................................................................................2411.001.00--------------------------------------------1.00-----------------------------------------------
..................................................................................................................................................................................................................TTCAAATCGGCCTGGATGTTTGAGGCG.............271.000.00-------------------------------------------------1.00------------------------------------------
...........GTTGTGGGACCAGGGATGTGAAGCAAGTACA................................................................................................................................................................................................................3111.001.00----1.00---------------------------------------------------------------------------------------
.......................................................................................................................AAGGATTGTGTGTGTTAT.................................................................................................................181.000.00-----------------------------------------------------------------------------1.00--------------
...................................................................................TGAGGAAGCCCCTGCCAA.....................................................................................................................................................181.000.00--------------------------1.00-----------------------------------------------------------------
.......................................................................................................................................................................................................................ATCGGCCTGGATGTTTGAGGTGTTTCATAGGGG..3311.001.00----1.00---------------------------------------------------------------------------------------
.................................................................................................................................................................................................................CTTCAAATCGGCCTGGAT.......................1811.001.00---------------1.00----------------------------------------------------------------------------
................................................................TGGTTTGGGAGAGCCGGCCGAGG...................................................................................................................................................................231.000.00---------------------------------------------------------------1.00----------------------------
....................................................................................................................................................................................................................CAAATCGGCCTGGATG......................1611.001.00----------------------------------1.00---------------------------------------------------------
...............................AAGCAAGTACATCAACCTT........................................................................................................................................................................................................1911.001.00---------------------------------------1.00----------------------------------------------------
............................................................................................................................TTGTGTGTGTGTGTGTGTGTGTGCTGG...................................................................................................271.000.00------------------------------------------------------------1.00-------------------------------
............................................................................................................................................................................GACTTGCCTTTGTGCTTGCCGTTTGTAGA.................................................291.000.00------------------------------------1.00-------------------------------------------------------
.............TGTGGGACCAGGGATGTG...........................................................................................................................................................................................................................1811.001.00----------------------1.00---------------------------------------------------------------------
..............GTGGGACCAGGGATGTGAAGCA......................................................................................................................................................................................................................2211.001.00----1.00---------------------------------------------------------------------------------------
.....................................................................................................................................................................................................................................TTGAGGTGTTTCATAAG....171.000.00--------------------------------1.00-----------------------------------------------------------
.........................................................GCGCAGTTGGTTTGGGAGAGCCGG.........................................................................................................................................................................2411.001.00---------------------------------------------------------1.00----------------------------------
....................................................................................................CAAGGCTGCCCACAGACG....................................................................................................................................181.000.00---------------------------------------------------------------------------------1.00----------
.............................................................................................CCTGCCCCAAGGCTGCCCACAGAGG....................................................................................................................................2511.001.00----------------1.00---------------------------------------------------------------------------
.....................................................................................................................GGAAGGATTGTGTGTGTG...................................................................................................................1840.750.75-------------------------------------------------------------------------------------0.75------
......................................................................................................................GAAGGATTGTGTGTGTGT..................................................................................................................1870.570.57--0.43----------------------------------------------------------------------------------------0.14
.....................................................................................................AAGGCTGCCCACAGAGA....................................................................................................................................1720.508.50---------------------------------------------------------------------------------------0.50----
.....................................................................................................................GGAAGGATTGTGTGTGTGTG.................................................................................................................2040.250.25--------------------------------------0.25-----------------------------------------------------

Antisense strand
AACTGCTCCATGTTGTGGGACCAGGGATGTGAAGCAAGTACATCAACCTTGAAACGCGCGCAGTTGGTTTGGGAGAGCCGGCCTGAGGAAGCCCCTGCCCCAAGGCTGCCCACAGAGGGAAGGATTGTGTGTGTGTGTGTGTGTGTGCCATCTTGACAGAGTGAGTCAGTCTGACTTGCCTTTGTGCTTGCCGTTTGTAGGTATCAGTGCTTCAAATCGGCCTGGATGTTTGAGGTGTTTCATAGGGGCT
.......................................................((...((((((((((....)))))))).)).....))....(((....)))..(((.....)))...................................................................................................................................
..................................................51.....................................................................122..............................................................................................................................
SizePerfect hitTotal NormPerfect NormSRR207110(GSM721072)
Nuclear RNA. (cell line)		SRR314796(SRX084354)
"Total RNA, fractionated (15-30nt)". (cell line)		SRR207115(GSM721077)
XRN1&2 knockdown. (XRN1/XRN2 cell line)		DRR000558(DRX000316)
"THP-1 whole cell RNA, after 3 day treatment . (cell line)		SRR553572(SRX182778)
source: Frontal Cortex. (Frontal Cortex)		SRR553573(SRX182779)
source: Cerebellum. (Cerebellum)		SRR444067(SRX128915)
Sample 24cDNABarcode: AF-PP-340: ACG CTC TTC . (cell line)		SRR342898(SRX096794)
small RNA seq of Right atrial tissue. (heart)		SRR342896(SRX096792)
small RNA seq of Right atrial tissue. (heart)		SRR033712(GSM497057)
Burkitt Lymphoma (BL510). (B cell)		TAX577746(Rovira)
total RNA. (breast)		SRR330888(SRX091726)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR342901(SRX096797)
small RNA seq of Left atrial tissue. (heart)		SRR033710(GSM497055)
GCB DLBCL (GCB385). (B cell)		SRR015364(GSM380329)
Plasma B cells (PC44). (B cell)		SRR033727(GSM497072)
HIV-positive DL (HIV412). (B cell)		DRR000559(DRX000317)
"THP-1 whole cell RNA, no treatment". (cell line)		SRR330868(SRX091706)
tissue: skin psoriatic involveddisease state:. (skin)		SRR342900(SRX096796)
small RNA seq of Right atrial tissue. (heart)		SRR444052(SRX128900)
Sample 12cDNABarcode: AF-PP-340: ACG CTC TTC . (skin)		SRR330879(SRX091717)
tissue: skin psoriatic involveddisease state:. (skin)		DRR000555(DRX000313)
"THP-1 whole cell RNA, no treatment". (cell line)		SRR039620(GSM531983)
HBV(+) Adjacent Tissue Sample 2. (liver)		SRR330922(SRX091760)
tissue: normal skindisease state: normal. (skin)		SRR330903(SRX091741)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR326279(GSM769509)
cytoplasmic fraction was isolated using PARIS. (cell line)		SRR189782TAX577740(Rovira)
total RNA. (breast)		SRR038860(GSM458543)
MM426. (cell line)		SRR330877(SRX091715)
tissue: skin psoriatic involveddisease state:. (skin)		SRR330858(SRX091696)
tissue: skin psoriatic involveddisease state:. (skin)		SRR330866(SRX091704)
tissue: skin psoriatic involveddisease state:. (skin)		TAX577745(Rovira)
total RNA. (breast)		SRR330906(SRX091744)
tissue: normal skindisease state: normal. (skin)		SRR330874(SRX091712)
tissue: skin psoriatic involveddisease state:. (skin)		SRR330915(SRX091753)
tissue: normal skindisease state: normal. (skin)		SRR330921(SRX091759)
tissue: normal skindisease state: normal. (skin)		SRR363675(GSM830252)
cell type: human foreskin fibroblasts (HFF)tr. (fibroblast)		SRR330902(SRX091740)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR363674(GSM830251)
cell type: human foreskin fibroblasts (HFF)tr. (fibroblast)		SRR330876(SRX091714)
tissue: skin psoriatic involveddisease state:. (skin)		SRR330867(SRX091705)
tissue: skin psoriatic involveddisease state:. (skin)		SRR330889(SRX091727)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR330872(SRX091710)
tissue: skin psoriatic involveddisease state:. (skin)		SRR037936(GSM510474)
293cand1. (cell line)		TAX577580(Rovira)
total RNA. (breast)		SRR107297(GSM677704)
18-30nt fraction of small RNA. (cell line)		DRR000562(DRX000320)
Isolation of RNA following immunoprecipitatio. (ago3 cell line)		SRR330892(SRX091730)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR189787SRR330863(SRX091701)
tissue: skin psoriatic involveddisease state:. (skin)		SRR330890(SRX091728)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR330891(SRX091729)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR060984(GSM569188)
Human plasma cell [09-001]. (cell line)		SRR037937(GSM510475)
293cand2. (cell line)		SRR330911(SRX091749)
tissue: normal skindisease state: normal. (skin)		SRR060982(GSM569186)
Human centrocyte [09-001]. (cell line)		SRR444048(SRX128896)
Sample 8cDNABarcode: AF-PP-333: ACG CTC TTC C. (skin)		SRR330887(SRX091725)
tissue: skin psoriatic uninvolveddisease stat. (skin)		TAX577739(Rovira)
total RNA. (breast)		SRR189779(GSM714639)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR330917(SRX091755)
tissue: normal skindisease state: normal. (skin)		SRR330896(SRX091734)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR189780(GSM714640)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR363676(GSM830253)
cell type: human foreskin fibroblasts (HFF)tr. (fibroblast)		SRR330901(SRX091739)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR191619(GSM715729)
166genomic small RNA (size selected RNA from . (breast)		TAX577744(Rovira)
total RNA. (breast)		TAX577589(Rovira)
total RNA. (breast)		SRR038863(GSM458546)
MM603. (cell line)		SRR191585(GSM715695)
196genomic small RNA (size selected RNA from . (breast)		SRR191582(GSM715692)
95genomic small RNA (size selected RNA from t. (breast)		SRR015360(GSM380325)
Plasma B cells (PC137). (B cell)		TAX577738(Rovira)
total RNA. (breast)		SRR342895(SRX096791)
small RNA seq of Left atrial tissue. (heart)		SRR191623(GSM715733)
18genomic small RNA (size selected RNA from t. (breast)		SRR342897(SRX096793)
small RNA seq of Left atrial tissue. (heart)		SRR039619(GSM531982)
HBV(+) HCC Tissue Sample 1. (liver)		SRR330920(SRX091758)
tissue: normal skindisease state: normal. (skin)		SRR330916(SRX091754)
tissue: normal skindisease state: normal. (skin)		SRR191527(GSM715637)
128genomic small RNA (size selected RNA from . (breast)		SRR039637(GSM518474)
THP1_total_sRNAs. (cell line)		TAX577743(Rovira)
total RNA. (breast)		SRR191622(GSM715732)
175genomic small RNA (size selected RNA from . (breast)		SRR029125(GSM416754)
U2OS. (cell line)		SRR207116(GSM721078)
Nuclear RNA. (cell line)		DRR000556(DRX000314)
"THP-1 whole cell RNA, after 3 day treatment . (cell line)		SRR330884(SRX091722)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR330912(SRX091750)
tissue: normal skindisease state: normal. (skin)		SRR330913(SRX091751)
tissue: normal skindisease state: normal. (skin)		DRR000557(DRX000315)
"THP-1 whole cell RNA, after 3 day treatment . (cell line)		SRR033721(GSM497066)
Ly3 cell line (Ly3). (B cell)
....................................................................................GAGGAAGCCCCTGCCTGT.................................................................................................................................................... 181.000.00-----1.00--------------------------------------------------------------------------------------
..............................GAAGCAAGTACATCATAG.......................................................................................................................................................................................................... 181.000.00--------------------------1.00-----------------------------------------------------------------
............................................................................................................................................................................................................CAGTGCTTCAAATCGTAGT........................... 191.000.00-----------------------------------------------------------1.00--------------------------------