ID: uc008irh.13
GENE: Grin1(6)
chr2:25160683-25160854-


(18)
AGO2.ip
(42)
BRAIN
(2)
CELL-LINE
(3)
DCR.mut
(2)
DGCR8.mut
(3)
EMBRYO
(4)
ESC
(1)
FIBROBLAST
(1)
LIVER
(1)
LUNG
(6)
OTHER
(3)
PIWI.ip
(11)
TESTES
(1)
TOTAL-RNA

Sense strand
CGTCGGGGAGCGCGAGATCTCTGGGAATGCCCTGCGCTACGCTCCTGACGGTGAGTGCTGGGCTGTGGGGTCGCCTGGGACTGTAGGCCAGTCCAGTCTCTAAGCTCCAGGGTCCCGCGCAGGCATCATCGGACTTCAGCTAATCAACGGCAAGAACGAGTCGGCCCACATC
...........................................................(((((.((((((.....(((((((..((....)))))))))...)))))).))))).........................................................
..........................................................59.............................................................122................................................
SizePerfect hitTotal NormPerfect NormSRR306542(GSM750585)
19-24nt. (ago2 brain)
SRR306535(GSM750578)
19-24nt. (ago2 brain)
SRR306541(GSM750584)
19-24nt. (ago2 brain)
SRR038742(GSM527277)
small RNA-Seq. (dgcr8 brain)
SRR306532(GSM750575)
19-24nt. (ago2 brain)
SRR306534(GSM750577)
19-24nt. (ago2 brain)
SRR306536(GSM750579)
19-24nt. (ago2 brain)
SRR306533(GSM750576)
19-24nt. (ago2 brain)
SRR553583(SRX182789)
source: Cerebellum. (Cerebellum)
SRR038744(GSM527279)
small RNA-Seq. (brain)
SRR306531(GSM750574)
19-24nt. (ago2 brain)
SRR306529(GSM750572)
19-24nt. (ago2 brain)
SRR306530(GSM750573)
19-24nt. (ago2 brain)
SRR306540(GSM750583)
19-24nt. (ago2 brain)
SRR306537(GSM750580)
19-24nt. (ago2 brain)
SRR039610(GSM527274)
small RNA-Seq. (brain)
SRR306544(GSM750587)
19-24nt. (ago2 brain)
SRR346417(SRX098258)
Global profiling of miRNA and the hairpin pre. (Brain)
mjTestesWT4()
Testes Data. (testes)
SRR306527(GSM750570)
19-24nt. (ago2 brain)
SRR038740(GSM527275)
small RNA-Seq. (dicer brain)
GSM314557(GSM314557)
ESC dgcr8 (Illumina). (ESC)
SRR346423(SRX098265)
Global profiling of miRNA and the hairpin pre. (Brain)
GSM510444(GSM510444)
brain_rep5. (brain)
SRR037906(GSM510442)
brain_rep3. (brain)
SRR553582(SRX182788)
source: Brain. (Brain)
SRR206939(GSM723280)
other. (brain)
SRR206942(GSM723283)
other. (brain)
SRR345198(SRX097259)
source: size fractionated RNA from mouse hipp. (brain)
SRR306543(GSM750586)
19-24nt. (ago2 brain)
SRR206941(GSM723282)
other. (brain)
SRR306526(GSM750569)
19-24nt. (ago2 brain)
SRR038741(GSM527276)
small RNA-Seq. (brain)
SRR546155(SRX180174)
Global profiling of miRNA and the hairpin pre. (ago2 Neuroblastoma)
SRR279906(GSM689056)
cell type: mouse embryonic stem cellcell line. (ESC)
SRR206940(GSM723281)
other. (brain)
SRR345196(SRX097257)
source: size fractionated RNA from mouse hipp. (brain)
SRR391852(GSM852147)
gender: femalegenotype/variation: ADAR2-/-tis. (embryo)
SRR038745(GSM527280)
small RNA-Seq. (dgcr8 brain)
SRR345200(SRX097261)
source: size fractionated RNA from mouse hipp. (brain)
SRR037905(GSM510441)
brain_rep2. (brain)
SRR037903(GSM510439)
testes_rep4. (testes)
SRR306539(GSM750582)
19-24nt. (ago2 brain)
SRR037901(GSM510437)
testes_rep2. (testes)
SRR040488(GSM533911)
E18.5 wild type LIVER small RNA. (liver)
SRR073955(GSM629281)
total RNA. (blood)
mjTestesWT2()
Testes Data. (testes)
SRR391845(GSM852140)
gender: femalegenotype/variation: wild typeti. (embryo)
SRR279903(GSM689053)
cell type: mouse embryonic stem cellcell line. (ESC)
SRR023850(GSM307159)
ZHBT-c40hsmallrna_rep1. (cell line)
SRR345201(SRX097262)
source: size fractionated RNA from mouse hipp. (brain)
GSM509280(GSM509280)
small RNA cloning by length. (testes)
SRR346425(SRX098267)
Global profiling of miRNA and the hairpin pre. (Neuroblastoma)
SRR039186(GSM485326)
shLuc.1309;ROSA-rtTA (+dox). (fibroblast)
SRR394084(GSM855970)
background strain: C57BL6/SV129cell type: KRa. (dicer cell line)
SRR034118(GSM466728)
Mili IP_Tdrd9+/- replicate1. (mili testes)
SRR038743(GSM527278)
small RNA-Seq. (dicer brain)
SRR034119(GSM466729)
Mili IP_Tdrd9+/- replicate2. (mili testes)
SRR346419(SRX098260)
Global profiling of miRNA and the hairpin pre. (Lung)
SRR391848(GSM852143)
gender: femalegenotype/variation: wild typeti. (embryo)
SRR069811(GSM610967)
small RNA sequencing; sample 3. (testes)
SRR037902(GSM510438)
testes_rep3. (testes)
SRR402766(SRX117949)
source: ES PGK female cells. (ESC)
SRR345202(SRX097263)
source: size fractionated RNA from mouse hipp. (brain)
SRR034120(GSM466730)
Mili IP_Tdrd9-/- replicate1. (mili testes)
SRR060845(GSM561991)
total RNA. (brain)
SRR037904(GSM510440)
brain_rep1. (brain)
SRR042477(GSM539869)
mouse brain tissue [09-002]. (brain)
SRR346421(SRX098263)
Global profiling of miRNA and the hairpin pre. (mixture)
SRR346424(SRX098266)
Global profiling of miRNA and the hairpin pre. (Neuroblastoma)
SRR069809(GSM610965)
small RNA sequencing; sample 1. (testes)
SRR037912(GSM510449)
newborn_rep5. (total RNA)
....................................................................................................TAAGCTCCAGGGTCCCGCGCAG..................................................221104.00104.0016.008.008.003.003.008.004.006.003.003.006.004.004.003.001.001.002.002.00-4.00-1.00--3.00-2.00--2.001.001.002.001.00-1.00------1.00-----------------------------
....................................................................................................TAAGCTCCAGGGTCCCGCGCAGT.................................................23177.00104.008.007.009.001.008.005.003.004.00--3.002.001.003.004.00-2.001.00-3.00-2.00-1.001.00-1.00--1.00-2.00---2.00----2.00---------------------1.00---------
....................................................................................................TAAGCTCCAGGGTCCCGCGC....................................................20133.0033.002.002.004.001.001.003.003.002.001.001.002.001.00-2.001.00-1.00------1.001.001.00----2.00-------1.00---------------------------------
....................................................................................................TAAGCTCCAGGGTCCCGCGCA...................................................21123.0023.001.002.001.001.001.00-4.001.001.00---2.00-1.00-2.00----1.001.002.00------------------1.00-----------------------1.00-----
....................................................................................................TAAGCTCCAGGGTCCCGCGCAGA.................................................2318.00104.00--1.001.00---------1.00--1.002.00-------1.001.00---------------------------------------------
...........................................................................................................................CATCATCGGACTTCAGCT...............................1818.008.00---------3.00-----2.00----3.00---------------------------------------------------
...............................................................TGTGGGGTCGCCTGGGACTGTAG......................................................................................2317.007.00--------4.00--------1.00---------------1.00------------------1.00-------------------
....................................................................................................TAAGCTCCAGGGTCCCGCGCAT..................................................2217.0023.00--1.001.00--1.00-1.00----1.001.00--------1.00------------------------------------------------
....................................................................................................TAAGCTCCAGGGTCCCGCGCT...................................................2116.0033.00-1.00--2.00--1.00----1.00--------------1.00--------------------------------------------
....................................................................................................TAAGCTCCAGGGTCCCGCGCTT..................................................2216.0033.001.00---------1.00-3.00-1.00---------------------------------------------------------
..........................................................................................................................GCATCATCGGACTTCAGCT...............................1915.005.00---1.00-----1.00----------1.00-------1.00---------1.00---------------------------------
....................................................................................................TAAGCTCCAGGGTCCCGCGCAGC.................................................2315.00104.00-1.00---------2.00-----1.00-------------------------------1.00----------------------
.............................................................................................................................TCATCGGACTTCAGCTAATCAACGG......................2513.003.00---------------2.00----1.00---------------------------------------------------
...............................................................TGTGGGGTCGCCTGGGACTGTAGT.....................................................................................2413.007.00-------------------------2.00-----------------------------------------1.00----
..................................................................................................................................................ACGGCAAGAACGAGTCG.........1712.002.00-2.00----------------------------------------------------------------------
....................................................................................................TAAGCTCCAGGGTCCCGCGCAA..................................................2212.0023.00----1.00-----------------1.00-------------------------------------------------
.................................................................................................................................CGGACTTCAGCTAATCAACGGCAAGAACG..............2912.002.00---2.00--------------------------------------------------------------------
..........................................................................................................................GCATCATCGGACTTCAGC................................1812.002.00--------------------1.00-----------------------------------1.00---------------
...............................................................TGTGGGGTCGCCTGGGACTGT........................................................................................2112.002.00-------1.00-------------------------------------------------------------1.00--
.........................................................................................................................GGCATCATCGGACTTCAGCT...............................2012.002.00---------------1.00----------------------1.00---------------------------------
.................................GCGCTACGCTCCTGACG..........................................................................................................................1712.002.00----------------------------------2.00-------------------------------------
....................................................................................................TAAGCTCCAGGGTCCCGCGCAGTT................................................2412.00104.00---------------------2.00--------------------------------------------------
....................................................GAGTGCTGGGCTGTGGGGT.....................................................................................................1912.002.00--------------------------------------------2.00---------------------------
...............................................................TGTGGGGTCGCCTGGGACTGTAGG.....................................................................................2412.002.00--------1.001.00--------------------------------------------------------------
...............................................................TGTGGGGTCGCCTGGGACTGTAGGTT...................................................................................2612.002.00----------------------1.00----------1.00--------------------------------------
............................................................................................................................ATCATCGGACTTCAGCT...............................1712.002.00---------1.00-----1.00--------------------------------------------------------
.....................................................................................................................................................GCAAGAACGAGTCGGCCCACATC2311.001.00---------------------------1.00--------------------------------------------
............................................................GGCTGTGGGGTCGCCACC..............................................................................................181.000.00---------------------------------------------1.00--------------------------
............................................................................................................................ATCATCGGACTTCAGCTAATCAACGGCAA...................2911.001.00-------------------------------------------------------1.00----------------
........................................................................................................CTCCAGGGTCCCGCGCAG..................................................1811.001.00-----------1.00------------------------------------------------------------
....................................................................................................TAAGCTCCAGGGTCCCGCGCTA..................................................2211.0033.00-----------1.00------------------------------------------------------------
.................................................................TGGGGTCGCCTGGGACTGTAGGTTT..................................................................................2511.001.00----------------------1.00-------------------------------------------------
...........................................................................................................................CATCATCGGACTTCAGCTAATCAACGG......................2711.001.00---1.00--------------------------------------------------------------------
..................................................GTGAGTGCTGGGCTGTGA........................................................................................................181.000.00----------------------1.00-------------------------------------------------
...........................................................................................................................CATCATCGGACTTCAGCTAATCAACG.......................2611.001.00--------------------------------1.00---------------------------------------
.........................AATGCCCTGCGCTACGC..................................................................................................................................1711.001.00----------------------------------1.00-------------------------------------
...........................................................................................................................CATCATCGGACTTCAGC................................1711.001.00---------------1.00--------------------------------------------------------
..................................................GTGAGTGCTGGGCTGTGGGGTCGCCTGGGACTGTAGGCCAGTCCAGTCTCTAAGCTCCAGGGTCCCGCGCAGT.................................................731.000.00--------------------------------------------------------------------1.00---
..........................................................................................................................GCATCATCGGACTTCTGTT...............................191.000.00----------------------------1.00-------------------------------------------
...............................................................................................................................ATCGGACTTCAGCTAATC...........................1811.001.00---------1.00--------------------------------------------------------------
..................................................GTGAGTGCTGGGCTGTGAA.......................................................................................................191.000.00--------1.00---------------------------------------------------------------
...............................................................TGTGGGGTCGCCTGGGACTGTTAT.....................................................................................2411.002.00--------1.00---------------------------------------------------------------
.........................................................................................................................................................GAACGAGTCGGCCCACATC1911.001.00---------------------------------------------------------------1.00--------
......................................ACGCTCCTGACGGTGATAGG..................................................................................................................201.000.00-------------1.00----------------------------------------------------------
.................................................GGTGAGTGCTGGGCTAGAG........................................................................................................191.000.00--------------------------------------------------1.00---------------------
..................................................................................................................................................ACGGCAAGAACGAGTCGGC.......1911.001.00----------------------------------------------------------1.00-------------
.............................................................................................................................TCATCGGACTTCAGCTGTGC...........................201.000.00---1.00--------------------------------------------------------------------
..................................................................................................................................GGACTTCAGCTAATCAACGGCAAGAACG..............2811.001.00---------------------------1.00--------------------------------------------
...................................................................................TAGGCCAGTCCAGTCTCTAAGCTCT................................................................251.000.00---1.00--------------------------------------------------------------------
..........CGCGAGATCTCTGGGAATGCC.............................................................................................................................................2111.001.00------------------------------1.00-----------------------------------------
..................................................................................................................................GGACTTCAGCTAATCAACGG......................2011.001.00---------------------------1.00--------------------------------------------
.................................................................TGGGGTCGCCTGGGACTGTAGG.....................................................................................2211.001.00---1.00--------------------------------------------------------------------
....................................................................................................TAAGCTCCAGGGTCCCGCGCTTT.................................................2311.0033.00-1.00----------------------------------------------------------------------
....................................................................................................TAAGCTCCAGGGTCCCGCGCATT.................................................2311.0023.00-1.00----------------------------------------------------------------------
...............................................................TGTGGGGTCGCCTGGGACTGTAT......................................................................................231.000.00-----------------------------1.00------------------------------------------
.................TCTCTGGGAATGCCCTGCGCT......................................................................................................................................2111.001.00------------------------------------------------1.00-----------------------
....................................................................................................................................................GGCAAGAACGAGTCGAGC......181.000.00---------------------------------------------1.00--------------------------
..........................................................................................................................GCATCATCGGACTTCAGCTAATC...........................2311.001.00---------1.00--------------------------------------------------------------
......................................................................................................................................................CAAGAACGAGTCGGCCCAC...1911.001.00-----------------------------------------------------------------------1.00
...........................................................................................................................CATCATCGGACTTCAGCTA..............................1911.001.00---1.00--------------------------------------------------------------------
..........................................................................................................................GCATCATCGGACTTCGGGC...............................191.000.00---------------------------------------1.00--------------------------------
........................................................................................................................................................AGAACGAGTCGGCCCACA..1811.001.00---1.00--------------------------------------------------------------------
....................................................GAGTGCTGGGCTGTGGG.......................................................................................................1720.500.50--------0.50---------------------------------------------------------------
.......................................................................CGCCTGGGACTGTAG......................................................................................1540.250.25--------0.25---------------------------------------------------------------
....................................................................................................TAAGCTCCAGGGTCC.........................................................1590.110.11-------0.11----------------------------------------------------------------

Antisense strand
CGTCGGGGAGCGCGAGATCTCTGGGAATGCCCTGCGCTACGCTCCTGACGGTGAGTGCTGGGCTGTGGGGTCGCCTGGGACTGTAGGCCAGTCCAGTCTCTAAGCTCCAGGGTCCCGCGCAGGCATCATCGGACTTCAGCTAATCAACGGCAAGAACGAGTCGGCCCACATC
...........................................................(((((.((((((.....(((((((..((....)))))))))...)))))).))))).........................................................
..........................................................59.............................................................122................................................
SizePerfect hitTotal NormPerfect NormSRR306542(GSM750585)
19-24nt. (ago2 brain)
SRR306535(GSM750578)
19-24nt. (ago2 brain)
SRR306541(GSM750584)
19-24nt. (ago2 brain)
SRR038742(GSM527277)
small RNA-Seq. (dgcr8 brain)
SRR306532(GSM750575)
19-24nt. (ago2 brain)
SRR306534(GSM750577)
19-24nt. (ago2 brain)
SRR306536(GSM750579)
19-24nt. (ago2 brain)
SRR306533(GSM750576)
19-24nt. (ago2 brain)
SRR553583(SRX182789)
source: Cerebellum. (Cerebellum)
SRR038744(GSM527279)
small RNA-Seq. (brain)
SRR306531(GSM750574)
19-24nt. (ago2 brain)
SRR306529(GSM750572)
19-24nt. (ago2 brain)
SRR306530(GSM750573)
19-24nt. (ago2 brain)
SRR306540(GSM750583)
19-24nt. (ago2 brain)
SRR306537(GSM750580)
19-24nt. (ago2 brain)
SRR039610(GSM527274)
small RNA-Seq. (brain)
SRR306544(GSM750587)
19-24nt. (ago2 brain)
SRR346417(SRX098258)
Global profiling of miRNA and the hairpin pre. (Brain)
mjTestesWT4()
Testes Data. (testes)
SRR306527(GSM750570)
19-24nt. (ago2 brain)
SRR038740(GSM527275)
small RNA-Seq. (dicer brain)
GSM314557(GSM314557)
ESC dgcr8 (Illumina). (ESC)
SRR346423(SRX098265)
Global profiling of miRNA and the hairpin pre. (Brain)
GSM510444(GSM510444)
brain_rep5. (brain)
SRR037906(GSM510442)
brain_rep3. (brain)
SRR553582(SRX182788)
source: Brain. (Brain)
SRR206939(GSM723280)
other. (brain)
SRR206942(GSM723283)
other. (brain)
SRR345198(SRX097259)
source: size fractionated RNA from mouse hipp. (brain)
SRR306543(GSM750586)
19-24nt. (ago2 brain)
SRR206941(GSM723282)
other. (brain)
SRR306526(GSM750569)
19-24nt. (ago2 brain)
SRR038741(GSM527276)
small RNA-Seq. (brain)
SRR546155(SRX180174)
Global profiling of miRNA and the hairpin pre. (ago2 Neuroblastoma)
SRR279906(GSM689056)
cell type: mouse embryonic stem cellcell line. (ESC)
SRR206940(GSM723281)
other. (brain)
SRR345196(SRX097257)
source: size fractionated RNA from mouse hipp. (brain)
SRR391852(GSM852147)
gender: femalegenotype/variation: ADAR2-/-tis. (embryo)
SRR038745(GSM527280)
small RNA-Seq. (dgcr8 brain)
SRR345200(SRX097261)
source: size fractionated RNA from mouse hipp. (brain)
SRR037905(GSM510441)
brain_rep2. (brain)
SRR037903(GSM510439)
testes_rep4. (testes)
SRR306539(GSM750582)
19-24nt. (ago2 brain)
SRR037901(GSM510437)
testes_rep2. (testes)
SRR040488(GSM533911)
E18.5 wild type LIVER small RNA. (liver)
SRR073955(GSM629281)
total RNA. (blood)
mjTestesWT2()
Testes Data. (testes)
SRR391845(GSM852140)
gender: femalegenotype/variation: wild typeti. (embryo)
SRR279903(GSM689053)
cell type: mouse embryonic stem cellcell line. (ESC)
SRR023850(GSM307159)
ZHBT-c40hsmallrna_rep1. (cell line)
SRR345201(SRX097262)
source: size fractionated RNA from mouse hipp. (brain)
GSM509280(GSM509280)
small RNA cloning by length. (testes)
SRR346425(SRX098267)
Global profiling of miRNA and the hairpin pre. (Neuroblastoma)
SRR039186(GSM485326)
shLuc.1309;ROSA-rtTA (+dox). (fibroblast)
SRR394084(GSM855970)
background strain: C57BL6/SV129cell type: KRa. (dicer cell line)
SRR034118(GSM466728)
Mili IP_Tdrd9+/- replicate1. (mili testes)
SRR038743(GSM527278)
small RNA-Seq. (dicer brain)
SRR034119(GSM466729)
Mili IP_Tdrd9+/- replicate2. (mili testes)
SRR346419(SRX098260)
Global profiling of miRNA and the hairpin pre. (Lung)
SRR391848(GSM852143)
gender: femalegenotype/variation: wild typeti. (embryo)
SRR069811(GSM610967)
small RNA sequencing; sample 3. (testes)
SRR037902(GSM510438)
testes_rep3. (testes)
SRR402766(SRX117949)
source: ES PGK female cells. (ESC)
SRR345202(SRX097263)
source: size fractionated RNA from mouse hipp. (brain)
SRR034120(GSM466730)
Mili IP_Tdrd9-/- replicate1. (mili testes)
SRR060845(GSM561991)
total RNA. (brain)
SRR037904(GSM510440)
brain_rep1. (brain)
SRR042477(GSM539869)
mouse brain tissue [09-002]. (brain)
SRR346421(SRX098263)
Global profiling of miRNA and the hairpin pre. (mixture)
SRR346424(SRX098266)
Global profiling of miRNA and the hairpin pre. (Neuroblastoma)
SRR069809(GSM610965)
small RNA sequencing; sample 1. (testes)
SRR037912(GSM510449)
newborn_rep5. (total RNA)
............................GCCCTGCGCTACGCTCCTGACGGTGA...................................................................................................................... 2617.007.00------------------7.00-----------------------------------------------------
.............GAGATCTCTGGGAATTTGT............................................................................................................................................ 195.000.00-----------------------------------------2.00-2.00-----------------1.00----------
...................................................................................TAGGCCAGTCCAGTCTCT....................................................................... 1814.004.00----------------------------1.00-------2.00---------------------------------1.00-
............................GCCCTGCGCTACGCTCCTGACGGTG....................................................................................................................... 2512.002.00----------------------------------------------2.00-------------------------
...............................CTGCGCTACGCTCCTGACGGT........................................................................................................................ 2112.002.00-----------------------------------------------1.00-----------1.00------------
.......................................................................................................................CAGGCATCATCGGACTTCAGCTAATCA.......................... 2712.002.00---------------------------------------------------------1.00------1.00-------
.........................................................................................................TCCAGGGTCCCGCGCAGGC................................................ 1911.001.00--------1.00---------------------------------------------------------------
.................................GCGCTACGCTCCTGACGGT........................................................................................................................ 1911.001.00-----------------------------------------------------1.00------------------
.....................................TACGCTCCTGACGGTGAG..................................................................................................................... 1811.001.00---------------------------------------1.00--------------------------------
...........................TGCCCTGCGCTACGCTCCTGAC........................................................................................................................... 2211.001.00---------------------------------------------------1.00--------------------
........................GAATGCCCTGCGCTACG................................................................................................................................... 171.000.00------1.00-----------------------------------------------------------------
.......................................CGCTCCTGACGGTGAGTGCTGGGC............................................................................................................. 2411.001.00-------------------------------------1.00----------------------------------
..................................CGCTACGCTCCTGACGGT........................................................................................................................ 1811.001.00------------------------------------------------1.00-----------------------
............................................................................................................................ATCATCGGACTTCAGA................................ 161.000.00----------------------------1.00-------------------------------------------
................................................................................................................TCCCGCGCAGGCAT.............................................. 1411.001.00---------------------------------------1.00--------------------------------
.........................................................CTGGGCTGTGGGGTCGCGG................................................................................................ 191.000.00------------------------------------------------------1.00-----------------
...................................................................................TAGGCCAGTCCAGTCTCTAAGC................................................................... 2211.001.00-------------------------------------1.00----------------------------------
............................GCCCTGCGCTACGCTCCTGACGGT........................................................................................................................ 2411.001.00-------------------------------------1.00----------------------------------
....................................................................................................................GCGCAGGCATCATCGGAC...................................... 181.000.00------------------------------------1.00-----------------------------------
.....................TGGGAATGCCCTGCGCTACGCTCCTG............................................................................................................................. 2611.001.00------------------------------------------------------------1.00-----------
..................CTCTGGGAATGCCCTGCGCTA..................................................................................................................................... 2111.001.00-----------------------------------------------1.00------------------------
.............................CCCTGCGCTACGCTCCTGACGG......................................................................................................................... 2211.001.00-----------------------------------------------------------------1.00------