ID: mmu-mir-7060
GENE: Fbs1(7)
chr7:134632715-134632951+


(1)
AGO.mut
(3)
AGO1.ip
(12)
AGO2.ip
(2)
AGO3.ip
(9)
B-CELL
(27)
BRAIN
(1)
CELL-LINE
(2)
DCR.mut
(3)
DGCR8.mut
(14)
EMBRYO
(8)
ESC
(6)
FIBROBLAST
(2)
HEART
(1)
KIDNEY
(5)
LIVER
(1)
LUNG
(4)
LYMPH
(20)
OTHER
(1)
OTHER.ip
(11)
OTHER.mut
(4)
PIWI.ip
(3)
PIWI.mut
(10)
SKIN
(10)
SPLEEN
(1)
TDRD1.ip
(36)
TESTES
(1)
THYMUS
(1)
UTERUS

Sense strand
GGCTGCTCTCTCCAACGGGGCCTTTGGAGGCCTGGGCAGCCCCACATTCAGTGAGTGCTGGGTAGAATGGGAAATGAGGGATGGAAGCTGCAACCGAGGGGCTATAGGCTCAAGCTGGGCTTGTGATGCTCTCTGTAGAGCATCCTCCTCTCGGCCCCTGTCACTTTCTACTCTACCTTCTACTCAGACTCCAGCGCCGTCTTTGCCCAGAAAGAAAGCCCAGGAGCCCCACCAGCC
...................................................((((((..(((((((.((((((.(((.((..((.........((((((((..((((((((.....))))))))((((((((....))))))))..)))))))))).)).))).)))))).)))))))..))))))...................................................
..................................................51......................................................................................................................................187................................................
SizePerfect hitTotal NormPerfect NormSRR546155(SRX180174)
Global profiling of miRNA and the hairpin pre. (ago2 Neuroblastoma)
mjTestesWT4()
Testes Data. (testes)
SRR402760(SRX117943)
source: ES E14 male cells. (ESC)
SRR553583(SRX182789)
source: Cerebellum. (Cerebellum)
SRR402762(SRX117945)
source: ES E14 male cells. (ESC)
SRR346424(SRX098266)
Global profiling of miRNA and the hairpin pre. (Neuroblastoma)
SRR391852(GSM852147)
gender: femalegenotype/variation: ADAR2-/-tis. (embryo)
SRR391853(GSM852148)
gender: femalegenotype/variation: ADAR2-/-/AD. (embryo)
SRR248524(GSM733812)
cell type: Thy1+ spermatogonial stem cellstra. (testes)
SRR390297(GSM849855)
cell line: NIH-3T3cell type: fibroblastip ant. (fibroblast)
SRR042458(GSM539850)
mouse basophil cells [09-002]. (b cell)
mjTestesWT1()
Testes Data. (testes)
SRR073954(GSM629280)
total RNA. (blood)
SRR391851(GSM852146)
gender: femalegenotype/variation: wild typeti. (embryo)
Ago1IP812(Rui)
Quantitative functions of Argonaute proteins . (ago1 skin)
SRR040488(GSM533911)
E18.5 wild type LIVER small RNA. (liver)
SRR028732(GSM400969)
Mili-Tdrd1 KO associated. (mili testes)
mjLiverWT3()
Liver Data. (liver)
SRR042471(GSM539863)
mouse TFHS cells [09-002]. (spleen)
SRR059769(GSM562831)
Treg_Drosha. (spleen)
SRR248523(GSM733811)
cell type: Thy1+ spermatogonial stem cellstra. (testes)
SRR014235(GSM319959)
2 dpp total. (testes)
SRR553582(SRX182788)
source: Brain. (Brain)
SRR553602(SRX182808)
source: Sertoli cells. (Sertoli cells)
Ago2IP504(Rui)
Quantitative functions of Argonaute proteins . (ago2 skin)
SRR279907(GSM689057)
cell type: mouse embryonic stem cellcell line. (ESC)
SRR391848(GSM852143)
gender: femalegenotype/variation: wild typeti. (embryo)
mjLiverKO1()
Liver Data. (Zcchc11 liver)
SRR345197(SRX097258)
source: size fractionated RNA from mouse hipp. (brain)
SRR039184(GSM485324)
shLuc.1309 (+dox). (fibroblast)
mjTestesKO7()
Testes Data. (Zcchc11 testes)
SRR346423(SRX098265)
Global profiling of miRNA and the hairpin pre. (Brain)
mjTestesKO8()
Testes Data. (Zcchc11 testes)
mjLiverKO3()
Liver Data. (Zcchc11 liver)
SRR042463(GSM539855)
mouse natural killer cells [09-002]. (spleen)
SRR037921(GSM510459)
e12p5_rep3. (embryo)
Ago1IP517(Rui)
Quantitative functions of Argonaute proteins . (ago1 skin)
SRR042456(GSM539848)
mouse hematopoetic progenitor cells [09-002]. (bone marrow)
mjTestesKO6()
Testes Data. (Zcchc11 testes)
GSM509278(GSM509278)
small RNA cloning by length. (piwi testes)
SRR391850(GSM852145)
gender: femalegenotype/variation: ADAR2-/-/AD. (embryo)
SRR248527(GSM733815)
cell type: spermatogonial stem cell enriched . (testes)
SRR306537(GSM750580)
19-24nt. (ago2 brain)
SRR042459(GSM539851)
mouse neutrophil cells replicate 1 [09-002]. (blood)
GSM509279(GSM509279)
MVH-/- E16.5 small RNA. (testes)
SRR306542(GSM750585)
19-24nt. (ago2 brain)
SRR402761(SRX117944)
source: ES E14 male cells. (ESC)
SRR042470(GSM539862)
mouse Th17 cells [09-002]. (lymph)
SRR553584(SRX182790)
source: Heart. (Heart)
SRR553586(SRX182792)
source: Testis. (testes)
SRR039183(GSM485323)
shLuc.1309 (-dox). (fibroblast)
SRR039185(GSM485325)
shLuc.1309;ROSA-rtTA (-dox). (fibroblast)
SRR346425(SRX098267)
Global profiling of miRNA and the hairpin pre. (Neuroblastoma)
SRR391845(GSM852140)
gender: femalegenotype/variation: wild typeti. (embryo)
SRR073955(GSM629281)
total RNA. (blood)
SRR402766(SRX117949)
source: ES PGK female cells. (ESC)
SRR345198(SRX097259)
source: size fractionated RNA from mouse hipp. (brain)
SRR038742(GSM527277)
small RNA-Seq. (dgcr8 brain)
SRR525238(SRA056111/SRX170314)
Second IgG control. (Blood)
GSM317183(GSM317183)
Ago-1 IP small RNAs from mouse brain. (ago1 brain)
SRR034118(GSM466728)
Mili IP_Tdrd9+/- replicate1. (mili testes)
SRR034119(GSM466729)
Mili IP_Tdrd9+/- replicate2. (mili testes)
GSM475280(GSM475280)
Mili-IP. (mili testes)
SRR042445(GSM539837)
mouse immature B cells [09-002]. (b cell)
SRR039186(GSM485326)
shLuc.1309;ROSA-rtTA (+dox). (fibroblast)
mjTestesWT2()
Testes Data. (testes)
SRR042454(GSM539846)
mouse germinal center B cells (lymph node) [09-002]. (b cell)
SRR525243(SRA056111/SRX170319)
Mus musculus domesticus miRNA sequencing. (ago2 Blood)
Ago3IP805(Rui)
Quantitative functions of Argonaute proteins . (ago3 skin)
SRR206941(GSM723282)
other. (brain)
SRR042469(GSM539861)
mouse Th2 cells [09-002]. (lymph)
SRR014236(GSM319960)
10 dpp total. (testes)
SRR042451(GSM539843)
mouse B cells activated in vitro with LPS/aIgD-Dextran [09-002]. (b cell)
SRR039152(GSM471929)
HL1_siNon_smallRNAseq. (muscle)
SRR038741(GSM527276)
small RNA-Seq. (brain)
SRR042473(GSM539865)
mouse nTreg cells [09-002]. (lymph)
SRR069809(GSM610965)
small RNA sequencing; sample 1. (testes)
mjTestesKO5()
Testes Data. (Zcchc11 testes)
SRR042472(GSM539864)
mouse iTreg cells [09-002]. (lymph)
SRR391846(GSM852141)
gender: femalegenotype/variation: ADAR2-/-tis. (embryo)
SRR051939(GSM545783)
Mov10L1-associated piRNAs. (mov10L testes)
SRR306528(GSM750571)
19-24nt. (ago2 brain)
SRR345196(SRX097257)
source: size fractionated RNA from mouse hipp. (brain)
SRR306536(GSM750579)
19-24nt. (ago2 brain)
SRR346421(SRX098263)
Global profiling of miRNA and the hairpin pre. (mixture)
Ago2IP517(Rui)
Quantitative functions of Argonaute proteins . (ago2 skin)
SRR059766(GSM562828)
DN3_Drosha. (thymus)
SRR040489(GSM533912)
E18.5 Ago2 catalytically inactive LIVER small RNA. (ago2 liver)
SRR391847(GSM852142)
gender: femalegenotype/variation: ADAR2-/-/AD. (embryo)
Ago3IP812(Rui)
Quantitative functions of Argonaute proteins . (ago3 skin)
SRR042450(GSM539842)
mouse B cells activated in vitro with LPS/IL4 replicate 3 [09-002]. (b cell)
SRR279905(GSM689055)
cell type: mouse embryonic stem cellcell line. (ESC)
SRR039154(GSM471931)
HL1_siSrf2_smallRNAseq. (muscle)
SRR391849(GSM852144)
gender: femalegenotype/variation: ADAR2-/-tis. (embryo)
SRR042455(GSM539847)
mouse plasma cells [09-002]. (blood)
SRR248526(GSM733814)
cell type: Thy1- spermatogonial stem cellstra. (testes)
SRR042465(GSM539857)
mouse CD4+ T cells (spleen) [09-002]. (spleen)
SRR029038(GSM433290)
25dpp_hetero_tdrd6-KO. (tdrd6 testes)
GSM509276(GSM509276)
small RNA cloning by length. (testes)
SRR042475(GSM539867)
mouse embryonic fibroblast cells [09-002]. (fibroblast)
SRR059771(GSM562833)
CD4_control. (spleen)
SRR345202(SRX097263)
source: size fractionated RNA from mouse hipp. (brain)
SRR037924(GSM510462)
e9p5_rep2. (embryo)
SRR029037(GSM433289)
18dpp_homo_tdrd6-KO. (tdrd6 testes)
SRR345204(SRX097265)
source: size fractionated RNA from mouse hipp. (brain)
SRR345199(SRX097260)
source: size fractionated RNA from mouse hipp. (brain)
SRR029036(GSM433288)
18dpp_hetero_tdrd6-KO. (tdrd6 testes)
SRR248525(GSM733813)
cell type: Thy1- spermatogonial stem cellstra. (testes)
SRR042449(GSM539841)
mouse B cells activated in vitro with LPS/IL4 replicate 2 [09-002]. (b cell)
RuiDGCR8KO(Rui)
DGCR8-dependent microRNA biogenesis is essent. (dgcr8 skin)
SRR042477(GSM539869)
mouse brain tissue [09-002]. (brain)
SRR038743(GSM527278)
small RNA-Seq. (dicer brain)
SRR023852(GSM307161)
ZHBT-c424hsmallrna_rep1. (cell line)
SRR042448(GSM539840)
mouse B cells activated in vitro with LPS/IL4 replicate 1 [09-002]. (b cell)
SRR059772(GSM562834)
CD4_Drosha. (spleen)
SRR042443(GSM539835)
mouse pro B cells [09-002]. (b cell)
SRR037920(GSM510458)
e12p5_rep2. (embryo)
SRR037905(GSM510441)
brain_rep2. (brain)
SRR042476(GSM539868)
mouse heart tissue [09-002]. (heart)
GSM509277(GSM509277)
small RNA cloning by length. (piwi testes)
SRR525244(SRA056111/SRX170320)
Mus musculus domesticus miRNA sequencing. (ago2 Blood)
SRR037922(GSM510460)
e12p5_rep4. (embryo)
SRR038745(GSM527280)
small RNA-Seq. (dgcr8 brain)
SRR014231(GSM319955)
16.5 dpc total. (testes)
SRR346420(SRX098261)
Global profiling of miRNA and the hairpin pre. (Uterus)
SRR279903(GSM689053)
cell type: mouse embryonic stem cellcell line. (ESC)
SRR014229(GSM319953)
10 dpp MILI. (mili testes)
SRR028730(GSM400967)
Tdrd1-associated. (tdrd1 testes)
SRR553604(SRX182810)
source: Spermatocytes. (Spermatocytes)
SRR306540(GSM750583)
19-24nt. (ago2 brain)
GSM314557(GSM314557)
ESC dgcr8 (Illumina). (ESC)
SRR306527(GSM750570)
19-24nt. (ago2 brain)
SRR346422(SRX098264)
Global profiling of miRNA and the hairpin pre. (Muscle)
SRR029042(GSM433294)
18.5dpc_hetero_tdrd1-KO. (tdrd1 testes)
SRR206940(GSM723281)
other. (brain)
SRR042447(GSM539839)
mouse mature B cells (spleen) replicate 2 [09-002]. (b cell)
SRR042457(GSM539849)
mouse mast cells [09-002]. (bone marrow)
GSM475281(GSM475281)
total RNA. (testes)
SRR038744(GSM527279)
small RNA-Seq. (brain)
SRR042487(GSM539879)
mouse BclXL B activated with LPS/IL4 [BalbC] [09-002]. (spleen)
SRR346414(SRX098255)
Global profiling of miRNA and the hairpin pre. (Spleen)
SRR037919(GSM510457)
e12p5_rep1. (embryo)
SRR345203(SRX097264)
source: size fractionated RNA from mouse hipp. (brain)
SRR042480(GSM539872)
mouse kidney tissue [09-002]. (kidney)
SRR039610(GSM527274)
small RNA-Seq. (brain)
SRR346419(SRX098260)
Global profiling of miRNA and the hairpin pre. (Lung)
SRR042467(GSM539859)
mouse T cells activated in vitro with Concanavalin A [09-002]. (spleen)
SRR059770(GSM562832)
Treg_Dicer. (spleen)
GSM509280(GSM509280)
small RNA cloning by length. (testes)
SRR306526(GSM750569)
19-24nt. (ago2 brain)
SRR029043(GSM433295)
18.5dpc_homo_tdrd1-KO. (tdrd1 testes)
SRR346415(SRX098256)
source: Testis. (Testes)
SRR042462(GSM539854)
mouse macrophages [09-002]. (bone marrow)
RuiDGCR8WT(Rui)
DGCR8-dependent microRNA biogenesis is essent. (skin)
RuiDcrWT(Rui)
DGCR8-dependent microRNA biogenesis is essent. (skin)
RuiDcrKO(Rui)
DGCR8-dependent microRNA biogenesis is essent. (dicer skin)
..................................................GTGAGTGCTGGGTAGAATGGGA.....................................................................................................................................................................22193.0093.005.00-12.006.00-4.003.001.001.009.002.00--3.002.00---2.001.001.00-2.002.00-----1.00--2.00-1.00-1.00-1.00-1.00-2.001.00-1.001.001.002.00-1.00----1.00-------1.00--1.001.00----1.00-----1.00--2.00---1.002.00-1.001.001.00-2.00-----1.00--1.00------1.00------1.00-----------------------------1.00----------
......................................................................................................................................................................TCTACTCTACCTTCTACTCAGT.................................................22170.005.0011.00-3.00-1.00-1.002.00--1.00-2.00-3.003.00-5.00-1.00----2.00----2.00---2.00-1.001.001.00--2.00-1.00------1.002.002.00-2.001.00----3.00--------------1.00----1.00---1.00---1.001.00-1.00------1.00-------------1.00----1.00----1.00---------1.00---------1.00--1.00--1.00--------
..................................................GTGAGTGCTGGGTAGAATGGG......................................................................................................................................................................21141.0041.006.0012.00---1.002.001.001.00-2.00-------3.00---1.001.003.00-1.00------------------1.00--------------------1.00-1.00----1.001.00-------------------1.00-1.00-----------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGG.......................................................................................................................................................................20140.0040.007.00--1.00-1.00----------------2.001.00-5.00-----3.001.00---------------1.00---2.00---2.003.002.00------1.00---1.00-------1.00-----1.00-----------1.00-----------------------------1.00----1.00---1.00--------------1.00------
..................................................GTGAGTGCTGGGTAGAATGGGAA....................................................................................................................................................................23137.0037.001.00--2.001.001.006.002.00-----1.00----------------2.00--2.002.00--2.00-------1.00------1.00--1.00-----------1.00-1.001.00-1.001.00-------------------------1.00--1.00-1.00--------1.00----1.00----------1.00---------1.00----------------
......................................................................................................................................................................TCTACTCTACCTTCTACTCAGA.................................................22132.0032.003.004.00-1.008.001.00-------1.001.00--1.00-1.00------1.00--------3.001.00---------2.00--------1.00--------------------------------------------------------1.00---------1.00-----------------1.00---------------
...................................................TGAGTGCTGGGTAGAATGGGAAATG.................................................................................................................................................................25113.0013.00--------2.00-------2.00---1.001.00-----------------1.00----1.00-----------------2.00--------1.00-----------------------------------------------1.00------1.00-----------------------------
..................................................GTGAGTGCTGGGTAGAATGGGAAA...................................................................................................................................................................24112.0012.00-----1.00-3.00--1.00--------1.00-------------------1.00-------1.00----------------1.00-----1.00----1.00-----------------1.00--------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGGGAAATG.................................................................................................................................................................2618.008.00--------1.00-----------1.00-----------1.00-----2.00--------------------------1.00-------------------------------------------------------------1.00-----1.00----------------------
..................................................GTGAGTGCTGGGTAGAATGGGAAT...................................................................................................................................................................2418.0037.002.00----1.00-2.00-----2.00---------1.00------------------------------------------------------------------------------------------------------------------------------------
........................................................................................TGCAACCGAGGGGCTATAGGCTCAAGCTGG.......................................................................................................................3017.007.00-----------7.00------------------------------------------------------------------------------------------------------------------------------------------------
...................................................TGAGTGCTGGGTAGAATGGGAAATGA................................................................................................................................................................2616.006.00----------------1.00---1.001.00-----------------1.00----1.00---------------------------------------------------------------------------------------------------------1.00-----
......................................................................................................................................................................TCTACTCTACCTTCTACTCAG..................................................2115.005.00----1.00----------------------4.00--------------------------------------------------------------------------------------------------------------------------------
.....................................................................................................................................................................TTCTACTCTACCTTCTACTCAGT.................................................235.000.003.00-----------1.00--------------------------------------1.00--------------------------------------------------------------------------------------------------------
........................................................................................TGCAACCGAGGGGCTATAGGCTCAAGC..........................................................................................................................2714.004.00--------------------------------------------------------------1.00--------------1.00-------------------------1.00------------------------1.00---------------------------
...................................................TGAGTGCTGGGTAGAATGGGAAAT..................................................................................................................................................................2414.004.00--------1.00---------------------1.00--------1.00----1.00---------------------------------------------------------------------------------------------------------------
.....................................................AGTGCTGGGTAGAATGGGAAATGAGGG.............................................................................................................................................................2714.004.00--------1.00--------------------------------1.00-----------------------------1.00-----------------------------------1.00------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGGGAT....................................................................................................................................................................2314.0093.00-----1.00---------------------------------------------------------1.00--------------------------------------------------------------------1.00---1.00-------------------
...................................................TGAGTGCTGGGTAGAATGGGAAA...................................................................................................................................................................2313.003.00----------1.00---------------------------------------------------------------------2.00---------------------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGGT......................................................................................................................................................................2113.0040.00---1.00---------------------------------------1.00------------------------1.00---------------------------------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGGGT.....................................................................................................................................................................2213.0041.001.00----1.00------------------------------------------------------------------------------------------------------------------1.00-----------------------------------
..................................................GTGAGTGCTGGGTAGAATGGGAAAT..................................................................................................................................................................2512.002.001.00--------------------1.00--------------------------------------------------------------------------------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGGGAAATA.................................................................................................................................................................2612.002.00------------------------------------------------------------1.001.00----------------------------------------------------------------------------------------------
.....................................................AGTGCTGGGTAGAATGGGAAATGAGG..............................................................................................................................................................2612.002.00--------2.00---------------------------------------------------------------------------------------------------------------------------------------------------
.....................................................AGTGCTGGGTAGAATGGGAAATGAGGGA............................................................................................................................................................2812.002.00----------------2.00-------------------------------------------------------------------------------------------------------------------------------------------
...................................................TGAGTGCTGGGTAGAATGGGAA....................................................................................................................................................................2212.002.00---------------------------------------------------------------------------------------------------------------------------------------1.00------------1.00-------
...................................................TGAGTGCTGGGTAGAATGGGA.....................................................................................................................................................................2112.002.001.00-------------1.00---------------------------------------------------------------------------------------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGAAT.....................................................................................................................................................................2212.001.00-------------------------------------------------2.00----------------------------------------------------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGGGAAAAA.................................................................................................................................................................2612.0012.00---------------2.00--------------------------------------------------------------------------------------------------------------------------------------------
......................................................................................................................................................................TCTACTCTACCTTCTACTCAGTT................................................2312.005.001.00------------------------------------------1.00----------------------------------------------------------------------------------------------------------------
........................TGGAGGCCTGGGCAGCCCCACA...............................................................................................................................................................................................2212.002.00--------------------------2.00---------------------------------------------------------------------------------------------------------------------------------
....................CCTTTGGAGGCCTGGGAA.......................................................................................................................................................................................................182.000.00----------------------------------------------------------------------------------2.00-------------------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGGA......................................................................................................................................................................2112.0040.001.00--1.00--------------------------------------------------------------------------------------------------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGGGAAATGA................................................................................................................................................................2712.002.00---------------------1.00--------1.00-----------------------------------------------------------------------------------------------------------------------------
......................................................................................................................................................................TCTACTCTACCTTCTACTCAGG.................................................2212.005.001.00------------------------------------------------------------------------------1.00----------------------------------------------------------------------------
..........TCCAACGGGGCCTTTCCT.................................................................................................................................................................................................................1831.670.33-------------------------------------------------------------------------------------------------------------0.33--------------------------------------------0.670.67
..................................................GTGAGTGCTGGGTAGAATGGGC.....................................................................................................................................................................2211.0041.00-------------------------------------------------------------------------------------1.00----------------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGGGAAATGT................................................................................................................................................................2711.008.00-----------------------------------------------1.00------------------------------------------------------------------------------------------------------------
.................................................................................TGGAAGCTGCAACCGAGGGGCTATAT..................................................................................................................................261.000.00------------------------------------------------------------1.00-----------------------------------------------------------------------------------------------
................................................................................................................AGCTGGGCTTGTGATGC............................................................................................................1711.001.00----------------------------------------------------------------------------------------------------------------------------------------------1.00-------------
..................................................GTGAGTGCTGGGTAGAATGGTA.....................................................................................................................................................................2211.0040.00-------------------------------------1.00----------------------------------------------------------------------------------------------------------------------
.........................................................................................GCAACCGAGGGGCTATAGGCT...............................................................................................................................2111.001.00-----------------------------------------------------1.00------------------------------------------------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAAT.........................................................................................................................................................................1811.001.00----------------------------------------------------------------1.00-------------------------------------------------------------------------------------------
..............................................................................................................................TGCTCTCTGTAGAGCATCCTCCTCTCGGCT.................................................................................301.000.00-------------------------------------------------------------1.00----------------------------------------------------------------------------------------------
.........................................................................................................................TGTGATGCTCTCTGTAGAGC................................................................................................2011.001.00-------------------------------------------------------------------------------------------1.00----------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGTG......................................................................................................................................................................2111.001.00---------------------------------------------------------------------------1.00--------------------------------------------------------------------------------
..........TCCAACGGGGCCTTTCCTT................................................................................................................................................................................................................1931.000.33--------------------------0.33----------------------------------------------------------------------------------0.67----------------------------------------------
................................................................................................................AGCTGGGCTTGTGATGCAGCC........................................................................................................2111.001.00----------------------------1.00-------------------------------------------------------------------------------------------------------------------------------
..........................................................................................................................................................................................................TTGCCCAGAAAGAAAGCCCAGGAGCCCC.......2811.001.00---------------------1.00--------------------------------------------------------------------------------------------------------------------------------------
....................................................GAGTGCTGGGTAGAATGGGAAT...................................................................................................................................................................221.000.00----------------------------------------------------------------------------------------------------1.00-------------------------------------------------------
.......................................................TGCTGGGTAGAATGGGAAATGAGGGA............................................................................................................................................................2611.001.00-------------------------------------------------------------1.00----------------------------------------------------------------------------------------------
.........................................................................................................................................................................ACTCTACCTTCTACTCAGT.................................................191.000.00-----------------------------1.00------------------------------------------------------------------------------------------------------------------------------
..............ACGGGGCCTTTGGAGGCCTGGGCAGCCC...................................................................................................................................................................................................2811.001.00-------------------------------------------------------------------------------------------1.00----------------------------------------------------------------
......................................................................................................................................................................TCTACTCTACCTTCTACTCAGC.................................................2211.005.00------------------------------------1.00-----------------------------------------------------------------------------------------------------------------------
..........................................................................TGAGGGATGGAAGCTGCAACCGAGG..........................................................................................................................................2511.001.00------------------------------------------------------------1.00-----------------------------------------------------------------------------------------------
.........................................................................................................................TGTGATGCTCTCTGTAGA..................................................................................................1811.001.00----------------------------------------------------------------1.00-------------------------------------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGA.......................................................................................................................................................................2011.001.00---1.00--------------------------------------------------------------------------------------------------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGGGAAATGAAA..............................................................................................................................................................2911.002.00--------------------1.00---------------------------------------------------------------------------------------------------------------------------------------
....................CCTTTGGAGGCCTGGGTTTT.....................................................................................................................................................................................................201.000.00---------------------------------------------------------------------------------------------------------------------1.00--------------------------------------
..................................................GTGAGTGCTGGGTAGAATGGGTT....................................................................................................................................................................2311.0041.00----------1.00-------------------------------------------------------------------------------------------------------------------------------------------------
................................................................................................................................CTCTCTGTAGAGCATCCTC..........................................................................................1911.001.00----------------------------------------------------------------------------------------------------------------------------1.00-------------------------------
.......................................................TGCTGGGTAGAATGGGAAATGA................................................................................................................................................................2211.001.001.00-----------------------------------------------------------------------------------------------------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGAG......................................................................................................................................................................2111.001.00---1.00--------------------------------------------------------------------------------------------------------------------------------------------------------
..........................................................................................................GGCTCAAGCTGGGCTTGTGATGT............................................................................................................231.000.00-----------------------------------------------------------------------------------------------------------------------------------------1.00------------------
..................................................GTGAGTGCTGGGTAGAATGGGAAAAAT................................................................................................................................................................2711.0012.00-------------------------------1.00----------------------------------------------------------------------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGGGAAAGG.................................................................................................................................................................2611.0012.00---------------------------------------------------------------------------------------------------------------------------1.00--------------------------------
............................................................................................................CTCAAGCTGGGCTTGTGATGCTCTC........................................................................................................2511.001.00--------------------------------------1.00---------------------------------------------------------------------------------------------------------------------
..........................................................................................................................................................................................GACTCCAGCGCCGTCCCT.................................181.000.00----------------------------------------------------------------------------------------------------------1.00-------------------------------------------------
..................................................GTGAGTGCTGGGTAGTGGG........................................................................................................................................................................191.000.00----------------------------1.00-------------------------------------------------------------------------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGGGAAATGAGGGAT...........................................................................................................................................................3211.001.00----------------------------------------------------------1.00-------------------------------------------------------------------------------------------------
...........................................................................................AACCGAGGGGCTATAGGCTC..............................................................................................................................2011.001.00-----------------------------------------------------------------------------------------1.00------------------------------------------------------------------
.............................................................................................................................................................................................TCCAGCGCCGTCTTTTTC..............................181.000.00--------------------------------------------------------1.00---------------------------------------------------------------------------------------------------
...................................................TGAGTGCTGGGTAGAATGGGAAATGAGGC.............................................................................................................................................................291.000.00-----------------------------------------1.00------------------------------------------------------------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGTGAA....................................................................................................................................................................2311.001.00-----------------------------------------------------------------------------------------------------------------------------------------------1.00------------
.............................................................................................................................................................................................TCCAGCGCCGTCTTTTGTT.............................191.000.00----------------------------1.00-------------------------------------------------------------------------------------------------------------------------------
.....................................................AGTGCTGGGTAGAATGGGAAATGAGGAA............................................................................................................................................................2811.002.00----------------1.00-------------------------------------------------------------------------------------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGTGA.....................................................................................................................................................................2211.001.00--------------------------------------------------------------------------------------------------------------1.00---------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGTGAT....................................................................................................................................................................2311.001.00---------------------------------------------------------------1.00--------------------------------------------------------------------------------------------
....................................................................GGGAAATGAGGGATGGAAGCTGCAACCGAGGGGCTATAGGCTCAAGCTGGGCTTGTGATGCTCTCTGTAGAGCATCCTCCTCTC.....................................................................................8411.001.00------------------------------------------------------------------------------------1.00-----------------------------------------------------------------------
...................................................................................................................................................................CTTTCTACTCTACCTTCTACTCAGT.................................................251.000.00---------------------------------------------------------------------------------------1.00--------------------------------------------------------------------
......................................................................................................................................................................TCTACTCTACCTTCTACTCA...................................................2011.001.00-----------------------------------------1.00------------------------------------------------------------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATAGGA.....................................................................................................................................................................2211.001.00--------------------------------------------------------------------------------------------------------------------------------------------------1.00---------
..................................................GTGAGTGCTGGGTAGAATGGGTAA...................................................................................................................................................................2411.0041.00--------------------------------------------------------------------------------------------------------------------------------------------------------1.00---
..................................................................................................................CTGGGCTTGTGATGCTCTCTGTAGAGCATCCTCCTCTCGGCCCCTGTCACTTTCTACTCTACCTTCTACTCAG..................................................7311.001.00------------------------------------------------------------------------------------1.00-----------------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATGGGATAT..................................................................................................................................................................2511.0093.00-------------------1.00----------------------------------------------------------------------------------------------------------------------------------------
..............ACGGGGCCTTTGGAGGC..............................................................................................................................................................................................................1711.001.00-------------------------------------------------------------------------------------------------------------------------------------------------------1.00----
.............................................................................................................................................................................................TCCAGCGCCGTCTTTGCC..............................1811.001.00---------------1.00--------------------------------------------------------------------------------------------------------------------------------------------
..................................................................................................................................CTCTGTAGAGCATCCTCCTCTCGGC..................................................................................2511.001.00----------------------------------------------------------------------------1.00-------------------------------------------------------------------------------
................................................................................................................................................................................................................AGAAAGAAAGCCCAGGAGCCCCACC....2511.001.00----------------------------------1.00-------------------------------------------------------------------------------------------------------------------------
..................................................................................................GGGCTATAGGCTCAATTGC........................................................................................................................191.000.00------------1.00-----------------------------------------------------------------------------------------------------------------------------------------------
..................................................GTGAGTGCTGGGTAGAATG........................................................................................................................................................................1911.001.00-----1.00------------------------------------------------------------------------------------------------------------------------------------------------------
....................CCTTTGGAGGCCTGGGAAGA.....................................................................................................................................................................................................201.000.00------------------------------------------------------------------------------1.00-----------------------------------------------------------------------------
.....................................................................................................................................................................TTCTACTCTACCTTCTACTCAGA.................................................2311.001.00----1.00-------------------------------------------------------------------------------------------------------------------------------------------------------
..........TCCAACGGGGCCTTTCC..................................................................................................................................................................................................................1730.670.33----------------------------------------0.33----------------------------------------------------------------------------------------------------------------0.33--
...........................................................................................................................................................................TCTACCTTCTACTCAGA.................................................1720.500.50----0.50-------------------------------------------------------------------------------------------------------------------------------------------------------
..........TCCAACGGGGCCTTT....................................................................................................................................................................................................................1530.330.33---------------------------------------------------------------------------------------------------------------------------------------------------------0.33--
...................................................................................................................................................................................................................AAGAAAGCCCAGGAGC..........1680.120.12---------------------------------------------------------------------------------------------0.12--------------------------------------------------------------

Antisense strand
GGCTGCTCTCTCCAACGGGGCCTTTGGAGGCCTGGGCAGCCCCACATTCAGTGAGTGCTGGGTAGAATGGGAAATGAGGGATGGAAGCTGCAACCGAGGGGCTATAGGCTCAAGCTGGGCTTGTGATGCTCTCTGTAGAGCATCCTCCTCTCGGCCCCTGTCACTTTCTACTCTACCTTCTACTCAGACTCCAGCGCCGTCTTTGCCCAGAAAGAAAGCCCAGGAGCCCCACCAGCC
...................................................((((((..(((((((.((((((.(((.((..((.........((((((((..((((((((.....))))))))((((((((....))))))))..)))))))))).)).))).)))))).)))))))..))))))...................................................
..................................................51......................................................................................................................................187................................................
SizePerfect hitTotal NormPerfect NormSRR546155(SRX180174)
Global profiling of miRNA and the hairpin pre. (ago2 Neuroblastoma)
mjTestesWT4()
Testes Data. (testes)
SRR402760(SRX117943)
source: ES E14 male cells. (ESC)
SRR553583(SRX182789)
source: Cerebellum. (Cerebellum)
SRR402762(SRX117945)
source: ES E14 male cells. (ESC)
SRR346424(SRX098266)
Global profiling of miRNA and the hairpin pre. (Neuroblastoma)
SRR391852(GSM852147)
gender: femalegenotype/variation: ADAR2-/-tis. (embryo)
SRR391853(GSM852148)
gender: femalegenotype/variation: ADAR2-/-/AD. (embryo)
SRR248524(GSM733812)
cell type: Thy1+ spermatogonial stem cellstra. (testes)
SRR390297(GSM849855)
cell line: NIH-3T3cell type: fibroblastip ant. (fibroblast)
SRR042458(GSM539850)
mouse basophil cells [09-002]. (b cell)
mjTestesWT1()
Testes Data. (testes)
SRR073954(GSM629280)
total RNA. (blood)
SRR391851(GSM852146)
gender: femalegenotype/variation: wild typeti. (embryo)
Ago1IP812(Rui)
Quantitative functions of Argonaute proteins . (ago1 skin)
SRR040488(GSM533911)
E18.5 wild type LIVER small RNA. (liver)
SRR028732(GSM400969)
Mili-Tdrd1 KO associated. (mili testes)
mjLiverWT3()
Liver Data. (liver)
SRR042471(GSM539863)
mouse TFHS cells [09-002]. (spleen)
SRR059769(GSM562831)
Treg_Drosha. (spleen)
SRR248523(GSM733811)
cell type: Thy1+ spermatogonial stem cellstra. (testes)
SRR014235(GSM319959)
2 dpp total. (testes)
SRR553582(SRX182788)
source: Brain. (Brain)
SRR553602(SRX182808)
source: Sertoli cells. (Sertoli cells)
Ago2IP504(Rui)
Quantitative functions of Argonaute proteins . (ago2 skin)
SRR279907(GSM689057)
cell type: mouse embryonic stem cellcell line. (ESC)
SRR391848(GSM852143)
gender: femalegenotype/variation: wild typeti. (embryo)
mjLiverKO1()
Liver Data. (Zcchc11 liver)
SRR345197(SRX097258)
source: size fractionated RNA from mouse hipp. (brain)
SRR039184(GSM485324)
shLuc.1309 (+dox). (fibroblast)
mjTestesKO7()
Testes Data. (Zcchc11 testes)
SRR346423(SRX098265)
Global profiling of miRNA and the hairpin pre. (Brain)
mjTestesKO8()
Testes Data. (Zcchc11 testes)
mjLiverKO3()
Liver Data. (Zcchc11 liver)
SRR042463(GSM539855)
mouse natural killer cells [09-002]. (spleen)
SRR037921(GSM510459)
e12p5_rep3. (embryo)
Ago1IP517(Rui)
Quantitative functions of Argonaute proteins . (ago1 skin)
SRR042456(GSM539848)
mouse hematopoetic progenitor cells [09-002]. (bone marrow)
mjTestesKO6()
Testes Data. (Zcchc11 testes)
GSM509278(GSM509278)
small RNA cloning by length. (piwi testes)
SRR391850(GSM852145)
gender: femalegenotype/variation: ADAR2-/-/AD. (embryo)
SRR248527(GSM733815)
cell type: spermatogonial stem cell enriched . (testes)
SRR306537(GSM750580)
19-24nt. (ago2 brain)
SRR042459(GSM539851)
mouse neutrophil cells replicate 1 [09-002]. (blood)
GSM509279(GSM509279)
MVH-/- E16.5 small RNA. (testes)
SRR306542(GSM750585)
19-24nt. (ago2 brain)
SRR402761(SRX117944)
source: ES E14 male cells. (ESC)
SRR042470(GSM539862)
mouse Th17 cells [09-002]. (lymph)
SRR553584(SRX182790)
source: Heart. (Heart)
SRR553586(SRX182792)
source: Testis. (testes)
SRR039183(GSM485323)
shLuc.1309 (-dox). (fibroblast)
SRR039185(GSM485325)
shLuc.1309;ROSA-rtTA (-dox). (fibroblast)
SRR346425(SRX098267)
Global profiling of miRNA and the hairpin pre. (Neuroblastoma)
SRR391845(GSM852140)
gender: femalegenotype/variation: wild typeti. (embryo)
SRR073955(GSM629281)
total RNA. (blood)
SRR402766(SRX117949)
source: ES PGK female cells. (ESC)
SRR345198(SRX097259)
source: size fractionated RNA from mouse hipp. (brain)
SRR038742(GSM527277)
small RNA-Seq. (dgcr8 brain)
SRR525238(SRA056111/SRX170314)
Second IgG control. (Blood)
GSM317183(GSM317183)
Ago-1 IP small RNAs from mouse brain. (ago1 brain)
SRR034118(GSM466728)
Mili IP_Tdrd9+/- replicate1. (mili testes)
SRR034119(GSM466729)
Mili IP_Tdrd9+/- replicate2. (mili testes)
GSM475280(GSM475280)
Mili-IP. (mili testes)
SRR042445(GSM539837)
mouse immature B cells [09-002]. (b cell)
SRR039186(GSM485326)
shLuc.1309;ROSA-rtTA (+dox). (fibroblast)
mjTestesWT2()
Testes Data. (testes)
SRR042454(GSM539846)
mouse germinal center B cells (lymph node) [09-002]. (b cell)
SRR525243(SRA056111/SRX170319)
Mus musculus domesticus miRNA sequencing. (ago2 Blood)
Ago3IP805(Rui)
Quantitative functions of Argonaute proteins . (ago3 skin)
SRR206941(GSM723282)
other. (brain)
SRR042469(GSM539861)
mouse Th2 cells [09-002]. (lymph)
SRR014236(GSM319960)
10 dpp total. (testes)
SRR042451(GSM539843)
mouse B cells activated in vitro with LPS/aIgD-Dextran [09-002]. (b cell)
SRR039152(GSM471929)
HL1_siNon_smallRNAseq. (muscle)
SRR038741(GSM527276)
small RNA-Seq. (brain)
SRR042473(GSM539865)
mouse nTreg cells [09-002]. (lymph)
SRR069809(GSM610965)
small RNA sequencing; sample 1. (testes)
mjTestesKO5()
Testes Data. (Zcchc11 testes)
SRR042472(GSM539864)
mouse iTreg cells [09-002]. (lymph)
SRR391846(GSM852141)
gender: femalegenotype/variation: ADAR2-/-tis. (embryo)
SRR051939(GSM545783)
Mov10L1-associated piRNAs. (mov10L testes)
SRR306528(GSM750571)
19-24nt. (ago2 brain)
SRR345196(SRX097257)
source: size fractionated RNA from mouse hipp. (brain)
SRR306536(GSM750579)
19-24nt. (ago2 brain)
SRR346421(SRX098263)
Global profiling of miRNA and the hairpin pre. (mixture)
Ago2IP517(Rui)
Quantitative functions of Argonaute proteins . (ago2 skin)
SRR059766(GSM562828)
DN3_Drosha. (thymus)
SRR040489(GSM533912)
E18.5 Ago2 catalytically inactive LIVER small RNA. (ago2 liver)
SRR391847(GSM852142)
gender: femalegenotype/variation: ADAR2-/-/AD. (embryo)
Ago3IP812(Rui)
Quantitative functions of Argonaute proteins . (ago3 skin)
SRR042450(GSM539842)
mouse B cells activated in vitro with LPS/IL4 replicate 3 [09-002]. (b cell)
SRR279905(GSM689055)
cell type: mouse embryonic stem cellcell line. (ESC)
SRR039154(GSM471931)
HL1_siSrf2_smallRNAseq. (muscle)
SRR391849(GSM852144)
gender: femalegenotype/variation: ADAR2-/-tis. (embryo)
SRR042455(GSM539847)
mouse plasma cells [09-002]. (blood)
SRR248526(GSM733814)
cell type: Thy1- spermatogonial stem cellstra. (testes)
SRR042465(GSM539857)
mouse CD4+ T cells (spleen) [09-002]. (spleen)
SRR029038(GSM433290)
25dpp_hetero_tdrd6-KO. (tdrd6 testes)
GSM509276(GSM509276)
small RNA cloning by length. (testes)
SRR042475(GSM539867)
mouse embryonic fibroblast cells [09-002]. (fibroblast)
SRR059771(GSM562833)
CD4_control. (spleen)
SRR345202(SRX097263)
source: size fractionated RNA from mouse hipp. (brain)
SRR037924(GSM510462)
e9p5_rep2. (embryo)
SRR029037(GSM433289)
18dpp_homo_tdrd6-KO. (tdrd6 testes)
SRR345204(SRX097265)
source: size fractionated RNA from mouse hipp. (brain)
SRR345199(SRX097260)
source: size fractionated RNA from mouse hipp. (brain)
SRR029036(GSM433288)
18dpp_hetero_tdrd6-KO. (tdrd6 testes)
SRR248525(GSM733813)
cell type: Thy1- spermatogonial stem cellstra. (testes)
SRR042449(GSM539841)
mouse B cells activated in vitro with LPS/IL4 replicate 2 [09-002]. (b cell)
RuiDGCR8KO(Rui)
DGCR8-dependent microRNA biogenesis is essent. (dgcr8 skin)
SRR042477(GSM539869)
mouse brain tissue [09-002]. (brain)
SRR038743(GSM527278)
small RNA-Seq. (dicer brain)
SRR023852(GSM307161)
ZHBT-c424hsmallrna_rep1. (cell line)
SRR042448(GSM539840)
mouse B cells activated in vitro with LPS/IL4 replicate 1 [09-002]. (b cell)
SRR059772(GSM562834)
CD4_Drosha. (spleen)
SRR042443(GSM539835)
mouse pro B cells [09-002]. (b cell)
SRR037920(GSM510458)
e12p5_rep2. (embryo)
SRR037905(GSM510441)
brain_rep2. (brain)
SRR042476(GSM539868)
mouse heart tissue [09-002]. (heart)
GSM509277(GSM509277)
small RNA cloning by length. (piwi testes)
SRR525244(SRA056111/SRX170320)
Mus musculus domesticus miRNA sequencing. (ago2 Blood)
SRR037922(GSM510460)
e12p5_rep4. (embryo)
SRR038745(GSM527280)
small RNA-Seq. (dgcr8 brain)
SRR014231(GSM319955)
16.5 dpc total. (testes)
SRR346420(SRX098261)
Global profiling of miRNA and the hairpin pre. (Uterus)
SRR279903(GSM689053)
cell type: mouse embryonic stem cellcell line. (ESC)
SRR014229(GSM319953)
10 dpp MILI. (mili testes)
SRR028730(GSM400967)
Tdrd1-associated. (tdrd1 testes)
SRR553604(SRX182810)
source: Spermatocytes. (Spermatocytes)
SRR306540(GSM750583)
19-24nt. (ago2 brain)
GSM314557(GSM314557)
ESC dgcr8 (Illumina). (ESC)
SRR306527(GSM750570)
19-24nt. (ago2 brain)
SRR346422(SRX098264)
Global profiling of miRNA and the hairpin pre. (Muscle)
SRR029042(GSM433294)
18.5dpc_hetero_tdrd1-KO. (tdrd1 testes)
SRR206940(GSM723281)
other. (brain)
SRR042447(GSM539839)
mouse mature B cells (spleen) replicate 2 [09-002]. (b cell)
SRR042457(GSM539849)
mouse mast cells [09-002]. (bone marrow)
GSM475281(GSM475281)
total RNA. (testes)
SRR038744(GSM527279)
small RNA-Seq. (brain)
SRR042487(GSM539879)
mouse BclXL B activated with LPS/IL4 [BalbC] [09-002]. (spleen)
SRR346414(SRX098255)
Global profiling of miRNA and the hairpin pre. (Spleen)
SRR037919(GSM510457)
e12p5_rep1. (embryo)
SRR345203(SRX097264)
source: size fractionated RNA from mouse hipp. (brain)
SRR042480(GSM539872)
mouse kidney tissue [09-002]. (kidney)
SRR039610(GSM527274)
small RNA-Seq. (brain)
SRR346419(SRX098260)
Global profiling of miRNA and the hairpin pre. (Lung)
SRR042467(GSM539859)
mouse T cells activated in vitro with Concanavalin A [09-002]. (spleen)
SRR059770(GSM562832)
Treg_Dicer. (spleen)
GSM509280(GSM509280)
small RNA cloning by length. (testes)
SRR306526(GSM750569)
19-24nt. (ago2 brain)
SRR029043(GSM433295)
18.5dpc_homo_tdrd1-KO. (tdrd1 testes)
SRR346415(SRX098256)
source: Testis. (Testes)
SRR042462(GSM539854)
mouse macrophages [09-002]. (bone marrow)
RuiDGCR8WT(Rui)
DGCR8-dependent microRNA biogenesis is essent. (skin)
RuiDcrWT(Rui)
DGCR8-dependent microRNA biogenesis is essent. (skin)
RuiDcrKO(Rui)
DGCR8-dependent microRNA biogenesis is essent. (dicer skin)
..............................................................................................................................................................................................................................GGAGCCCCACCAGCCACA 182.000.00------------1.00-----------------------------------------1.00-----------------------------------------------------------------------------------------------------
...........................................................................................................................................GCATCCTCCTCTCGGCGCC............................................................................... 191.000.00---------------------------------------------------------------------------------------------------------1.00--------------------------------------------------
.............................................................................................................................................................................................................................AGGAGCCCCACCAGCCCC 181.000.00------------1.00-----------------------------------------------------------------------------------------------------------------------------------------------
...................................................................................................................................................................CTTTCTACTCTACCTATGA....................................................... 191.000.00------------------------------------------------------------------------------------------------------------------------------------------1.00-----------------
..............................................................................................................................................................................................................................GGAGCCCCACCAGCCCCA 181.000.00------------1.00-----------------------------------------------------------------------------------------------------------------------------------------------
..............................................................................................................................................................................................................................GGAGCCCCACCAGCCAAA 181.000.00------------------------------------------------------1.00-----------------------------------------------------------------------------------------------------
............................................................................................................................................................................................CTCCAGCGCCGTCTTT................................. 1611.001.00----------------------------1.00-------------------------------------------------------------------------------------------------------------------------------
...................................................................................................................................................CTCTCGGCCCCTGTCAAAA....................................................................... 191.000.00----------------------------------------------------------------------------1.00-------------------------------------------------------------------------------
...............................................................................................................................................CCTCCTCTCGGCCCCGAAG........................................................................... 191.000.00------------------------------------------------------------------------------------------------------------------1.00-----------------------------------------
............................................................................................................................................................CCTGTCACTTTCTACAACA.............................................................. 191.000.00----1.00-------------------------------------------------------------------------------------------------------------------------------------------------------