dme-mir-991

dme-mir-991

This miRNA belongs to the dme-mir-992, dme-mir-991, and dme-mir-2498 cluster which is adjacent to to the dme-mir-310 - 313 cluster
No Changes
- One base within the miR region of the D. simulans ortholog was flagged as ambiguous
  - Upon resequencing this ortholog, the accuracy of the genomic base was confirmed.

Species

Chrom

Pos

Ref base

Type

A

C

G

T

RNAseq Total

A

C

G

T

Trace Total

Decision

Partition

droSim1

chr2R

15134425

T

3

0

0

0

1

29

0

0.583

0

0.417

12

Ambiguous - Suggested base change does not agree with neighboring species

miR

Uncorrected alignment

dme-mir-991

Species	Coordinate	Alignment
dm3	chr2R:16472690-16472815 -	GTAGGATATCAAAACTATCA-------CTGCAGTTTCAGG-----CTTTTCCCAACTACACCTATTAAT----ACATATTTTA------------------ACGTCCTATTAAAGTTGTAGTTTGGAAAGTTTTGGTTTTGCATTAGAAATC-GCCACTCA
droSim1	chr2R:15134383-15134509 -	GGGGTATATCAAAAATATCA-------CTGCAGTTTCAGG-----CTTTTCCCAACTACATCTATTAAT----ATTTATTTTA------------------ACGTCGTATTAAAGTTGAAGTTTGGAAAATTTTGGTTTTGCATTTGAAATCCGCCACTCA
droSec1	super_1:14027408-14027534 -	GGGGTATATCAAAAATATCA-------GTGCAGTTTCAGT-----CTTTTCCCAACTACATCTATTAAT----ACTTATTTTA------------------ACGTCGTATTAAAGTTGAAGTTTGGAAAATTTTGGTTTTGCATTTGAAATCCGCCACTCA
droYak2	chr2R:12723766-12723891 +	GGAGGATACAAAAAAATCGC-------CTGCAGTTTCAAG-----CTTTTCCCCACTCCTTGCTTTAAT----ACTTATTTTA------------------ACGTCGTATTAAAGCCAGAGTTTGGAAAGTTTTGATTCTGCATTAGAAATC-GCCACCCA
droEre2	scaffold_4845:10619033-10619157 -	GGAGAATACCAAA-CTAGCA-------CTGCAGCTTCAAG-----CTTTTCCCCACTCCTTGCTTTAGT----ACTTATTTTA------------------ATGTCGTATTAAAGCCAGAGTTTGGAAAGTTTTGATTCTGCTTTTTAAATC-GCCACCCA
droAna3	scaffold_13266:4131534-4131677 +	TATTACTATTACAAACATTGTGCAACACTGCCGAATTGGAGACAACTTGGCTGGAATCAATCCATTCATTCTTTTCAATTTTGGCAGCCGACGGGGTAAGACCTCCCTATT-------TAAGTGGTAAAGTGATTTCTTGGAATTAAAGAA----------
dp4	Unknown	-----------------------------------------------------------------------------------------------------------------------------------------------------------------
droPer1	Unknown	-----------------------------------------------------------------------------------------------------------------------------------------------------------------
droWil1	Unknown	-----------------------------------------------------------------------------------------------------------------------------------------------------------------
droVir3	Unknown	-----------------------------------------------------------------------------------------------------------------------------------------------------------------
droMoj3	Unknown	-----------------------------------------------------------------------------------------------------------------------------------------------------------------
droGri2	Unknown	-----------------------------------------------------------------------------------------------------------------------------------------------------------------