dme-mir-956

Changes

The T to C substitution in the star region of the D. ananassae and D. grimshawi orthologs are supported by all trace reads.
The reported D. persimilis ortholog reported in the 12-way alignment is wrong as shown below:

Here is the correct D. persimilis ortholog found by searching the persimilis trace reads with the D. pseudoobscura ortholog:

>dps-mir-956
ATGTGCACTTCTACTGATCGTTATCGTGTTTGGAATGGTCTCGTTAGCTAACGGATGAGCAACTGCTTGCGCGCATTGGCCAAATGCATTTCGCACCGGGATGGATGTGGATGTGGCTGGATGTGGTGTCCCCAAGCACAGCCAGGTGTAGTTTCGAGACCACTCTAATCCATTGCACCGCCCACCCATTTATGCAAA
>gnl|ti|732454354:721-914
ATGTGCACTTCTACTGATCGTTATCGTGTTTGGAATGGTCTCGTTAGCTAACGGATGAGCAACTGCTTGCGCGCATTGG-CAAATGCATTTCGCACCG-GATGGATGTGGATGTGGCTGGATGTG-TGTTCCCAAGCACAGCCA-GTGTAGTTTCGAGACCACTCTA-TCCATTGCACCGCCCACCCATTTATGCAAA

Corrected alignment:

dme-mir-956

Species	Coordinate	Alignment
dm3	chr3L:11766816-11766993 -	ATGAGC-ACTTTTCCTGATCGTTATCGTGTTTGGAATGGTCTCGTTAGCTAACGGATGAGCAAGTGCTCG-GCTCA-CTGGCCC---------------------AAATGCAGTTTGCC-C-GGAGA--------------CGCCGGT---------------TAACCCAG--------------CACTGAAATGTGTAGTTTCGAGACCACTCTAATCCATTGCAGCATTTTCAACAATATGCAAA
droSim1	chr3L:11132280-11132457 -	ATGTGC-TCTTTTCCTGATCGTTATCGTGTTTGGAATGGTCTCGTTAGCTAACGGATGAGCAAGTGCTCG-GCTCA-CTGGCCC---------------------AAATGCAGTTTGCC-C-GGAGA--------------CGCCGGT---------------TGCCCAAG--------------CACTGAAATGTGTAGTTTCGAGACCACTCTAATCCATTGCAGCATTTTCCACAATATGCAAA
droSec1	super_0:3951754-3951931 -	ATGAGC-TCTTTTCCTGATCGTTATCGTGTTTGGAATGGTCTCGTTAGCTAACGGATGAGCAAGTGCTCG-GCTCA-CTGGCCC---------------------AAATGCAGTTTGCC-C-GGAGA--------------CGCCGGT---------------TGCCCAAG--------------CACTGAAATGTGTAGTTTCGAGACCACTCTAATCCATTGCAGCATTTTCCACAATATGCTAA
droYak2	chr3L:11795661-11795838 -	ATGTGC-ACTTTTCCTGATCGTTATCGTGTTTGGAATGGTCTCGTTAGCTAACGGATGAGCAAGTGCTCG-GCTCA-CTGGCCC---------------------AAATGCAGTTTGCC-C-GGAGG--------------AGCCGGT---------------TTACCCAG--------------CACTCAAGTGTGTAGTTTCGAGACCACTCTAATCCATTGCACCATTTTCCACTATATGCAAA
droEre2	scaffold_4784:11744386-11744563 -	ATGTGC-ACTTTTCCTGATCGTTATCGTGTTTGGAATGGTCTCGTTAGCTAACGGATGAGCTAGTGCTCG-GCTCA-CCGGCCC---------------------AAATGCAGTTTGCC-C-GGAGA--------------TGCCGGT---------------TTCCTCAG--------------CACTCAAATGTGTAGTTTCGAGACCACTCTAATCCATTGCAGCATTTTCCACAATATGCAAA
droAna3	scaffold_13337:6020232-6020390 -	ATGTGC-ACTTTTCTAGATCGTTATCGCGTTTGGAATGGTCTCGTTAGCTAACGAATGGACTA------------------------------------------AAATGCAATTTGCA-A-TTCGG--------------CGCCAAT---------------TATCCAGG--------------CGCCAAAAAGTTTAGTTTCGAGACCACTCTAATCCATTGCATGATCTTCACTTGTATGCAAA
dp4	chrXR_group8:4341287-4341484 +	ATGTGC-ACTTCTACTGATCGTTATCGTGTTTGGAATGGTCTCGTTAGCTAACGGATGAGCAACTGCTTGCGCGCA-TTGGCC----------------------AAATGCATTTCGCA-C-CGGGATGGATGTG--GATG-------TGGCTGGATGTGGTGTCCCCAAG--------------CACAGCCAGGTGTAGTTTCGAGACCACTCTAATCCATTGCACCGCCCACCCATTTATGCAAA
droPer1	gnl\|ti\|732454354:721-914 +	ATGTGC-ACTTCTACTGATCGTTATCGTGTTTGGAATGGTCTCGTTAGCTAACGGATGAGCAACTGCTTGCGCGCA-TTGGC-----------------------AAATGCATTTCGCA-CCGGA--TGGATGTG--GATG-------TGGCTGGATGTG-TGTTCCCAAG--------------CACAGCC-AGTGTAGTTTCGAGACCACTCTA-TCCATTGCACCGCCCACCCATTTATGCAAA
droWil1	scaffold_180949:2569086-2569314 +	ATGTGC-AATTATACTGATCGTTATCGTGTTTGGAATGGTCTCGTTAGCTAACGGATGAACAAGTGCTTG-GGGCAATTGAGCCAAAAACAAAACAGAAAAAAGAAAATGCACTTTGCCAT-CAAAACGAATGTGCGAATATGCAATT---------------TGGCCAGGCTCCTCGAACATAACACTAAGAGGTGTAGTTTCGAGACCACTCTAATCCATTGCCACATTTTTCACAATATGCAAA
droVir3	scaffold_13049:23989392-23989532 -	ATGCGCGAAATTTAACGATCGTTATCGTGTTTGGAATGGTCTCGTTAGCTAACGGATGAACA---------------TTGACCA---------------------AAGCGCACCTT----------------------------------------------------------------------ACAGCGTAGTGTAGTTTCGAGACCACTCTAATCCATTGCATCGTTTCTGGGCATATGCAAA
droMoj3	scaffold_6680:18620871-18621011 -	ATGTGCGAAATTCAACGATCGTTATCGTGTTTGGAATGGTCTCGTTAGCTAACGGATGAACA---------------TTAACCA---------------------AAGCGCACCTT----------------------------------------------------------------------ACGGCGTAGTGTAGTTTCGAGACCACTCTAATCCATTGCACCGTTTCTTTCCATATGCAAA
droGri2	scaffold_15110:9704256-9704382 +	ATGTGCGAATCTAAGCGATCGTTATCGCGTTTGGAATGGTCTCGTTAGCTAACCAAAGCGCT--------------------------------------------------CGTT----------------------------------------------------------------------ACAGCGTAGTGTAGTTTCGAGACCACTCTAATCCATTCCATCGCTTTTCATTGTATGCAAA