dme-mir-4963
Changes
| Species | Chrom | Pos | Ref base | Type | A | C | G | T | RNAseq Total | A | C | G | T | Trace Total | Decision | Partition |
| droSim1 | chrX | 4425329 | G | 3 | 0 | 0 | 0 | 0 | 0 | 0.556 | 0 | 0.444 | 0 | 9 | Ambiguous - Suggested base change does not agree with neighboring species | Loop |
| droSim1 | chrX | 4425330 | C | 3 | 0 | 0 | 0 | 0 | 0 | 0.333 | 0.444 | 0.222 | 0 | 9 | Ambiguous - Suggested base change does not agree with neighboring species | Loop |
| droSim1 | chrX | 4425340 | A | 3 | 0 | 0 | 0 | 0 | 0 | 0.333 | 0 | 0 | 0.667 | 9 | Ambiguous- flagged as a genomic error but reseqencing confirmed the genomic base accuracy | miR |
| droSim1 | chrX | 4425348 | G | 3 | 0 | 0 | 0 | 0 | 0 | 0.556 | 0 | 0.444 | 0 | 9 | Ambiguous - Suggested base change does not agree with neighboring species | miR |
Corrected alignment
| Species | Coordinate | Alignment |
|---|---|---|
| dm3 | chrX:5684770-5684909 + | CACCCACTGCGAATTGCTGTAGGAAAT-------TTTTT-GGGGTACTTCTGGTTTCATAACGCAGAGAATCATC-----------AGCGTGAAGGAATGATTCTCTGCGGGCTGGAACCAGAAGTCCTGCAAATGTTCTTTTACGGATTTTAA---CCAGG |
| droSim1 | chrX:4425250-4425396 + | CACCCACTGCGAATTGCTATAGGAAATGATGGAGTTTTT-GGGGTACTTCTGGTTTCATACTGCAGAGAATCTTC-----------AGCGTGCAGTAATGATTTCCTGCGGGCTGCAACCAGAAGTCCTGCAGATGCTCTTTTACGGATTTTAA---CCAGG |
| droSec1 | super_4:1079282-1079434 - | CACCCACTGCGAATTGCTATAGGAAATGATGGAGTTTTT-GGGGTACTTCTGGTTTCATAACTCAGAGAATCTTCATGATTTCTTAATCA-----TAATGATTTCCTGCGGGCTGCAACCAGAAGTCCTGCAGATGCTCTTTTACGGATTTTAA---CCAGG |
| droYak2 | chrX:2788911-2789034 - | CACCCACTGCGAAATGCTATAGGACATGATGGAGCTTCTGGAGGTACTTCTGGTTTTATAATGCAGAGAATCGTA-----------ATCGTGAAGGAATGATTCTATGCGTACTGAAATCGGGAGTCCTGCAGAT--------------------------- |
| droEre2 | scaffold_4690:3033374-3033523 + | CACCCACTGCGAAATGCTATAGGAAACGATGGGGTTTCT-GGGGTACTTCTGGTTTTATAACGCACGGAATCTGA-----------ATCGTGAATGAATGATTCCTTGCGGACTGGAACCAGAAGTCCTGCAGAGGCTTCTTGAATGATTTGAAGTTTCGGA |
| droAna3 | scaffold_13117:1117754-1117783 + | CACCCACTGCGACGTACTTTAGAGAGTTGT------------------------------------------------------------------------------------------------------------------------------------ |
| dp4 | chr3:13862643-13862665 + | CACCAGCTGTGGGCTGCTTTAGG------------------------------------------------------------------------------------------------------------------------------------------- |
| droPer1 | super_4:1457976-1457998 - | CACCAACTGTGGGCTGCTTTAGG------------------------------------------------------------------------------------------------------------------------------------------- |
| droWil1 | scaffold_181096:7345062-7345085 - | AACCCACTGTGATGTCCTGTAAAA------------------------------------------------------------------------------------------------------------------------------------------ |
| droVir3 | scaffold_13324:2924689-2924720 + | CACGGCCTGTGGTGTGCTATAAATTTT------------------------------------------------------------------------------ATGTG---------------------------------------------------- |
| droMoj3 | scaffold_6496:13805954-13805976 - | CACCGACTGCGGCGTCCTCTGAG------------------------------------------------------------------------------------------------------------------------------------------- |
| droGri2 | scaffold_15112:3786307-3786359 + | CACCAGCTGCGGCGTGCTATAAATTC--------TTTTC-GTTGTATTTCTGGGTGTTCAAC---------------------------------------------------------------------------------------------------- |