dme-mir-309

dme-mir-309

This miRNA belongs to a cluster which includes dme-mir-309, dme-mir-3, dme-mir-286, dme-mir-4, dme-mir-5, dme-mir-6-1 - 6-3.
All the substitutions within the miR and miR* regions are supported by trace reads.
Changes
- One base within the D. simulans miR* region was flagged as ambiguous, however, upon resequencing the D. simulans ortholog we confirmed the accuracy of the genomic base.

Species

Chrom

Pos

Ref base

Type

A

C

G

T

RNAseq Total

A

C

G

T

Trace Total

Decision

Partition

droSim1

chr2R

14254691

C

3

0

1

0

0

31

0

0.353

0

0.647

17

Ambiguous - Suggested base change does not agree with neighboring species

miR*

Uncorrected Alignment:

dme-mir-309

Species	Coordinate	Alignment
dm3	chr2R:15549196-15549294 -	AA-------GTTT---GGAATAG-CAATTATACGACAAACCTTGTTCGGTTTTGCCA----------------ATTTCCA------AG-CCAGCACTGGGTAAAGTTTGTCCTATAATCCCGAAACGA--------------C-GACC
droSim1	chr2R:14254629-14254727 -	AA-------GTTT---GGAACAG-CAATTATACGACAAACCTTGTTCGGTTTTGCCA----------------ATTTCCA------AG-CCAGCACTGGGTAAAGTTTGTCCTATAATCCCGAAACGA--------------C-GACC
droSec1	super_1:13063923-13064021 -	AA-------GTTT---GGAACAG-CAATTATACGACAAACCTTGTTCGGTTTTGCCA----------------ATTTCCA------AG-CCAGCACTGGGTAAAGTTTGTCCTATAATCCCGAAACGA--------------C-GACC
droYak2	chr2R:13623487-13623590 +	AA--GCAAAGTTT---GGAACAG-CAATTATACGACAAACCTTGTTCTGTTTTGCCA----------------ATTTCCT------AG-CCAGCACTGGGTAAAGTTTGTCCTATAATCCCGAAACGA--------------C-GACC
droEre2	scaffold_4845:9749440-9749538 -	GA-------GTTT---GGAACAG-CAATTATACGACAAACCTTGTTCGGTTTTGCCA----------------ATTTCCT------AG-CCAGCACTGGGTAAAGTTTGTCCTATAATCCCGAAACGA--------------C-GACC
droAna3	scaffold_13266:9731105-9731197 +	GA-----------------AGAT-C-ATTATACGACAAACCTTGTTCGGTTTTGCCA----------------ATTTCCT------A--GCAGCACTGGGTAAAGTTTGTCCTATAATCTATAAAAAA--------G-----CCGTCC
dp4	chr3:12677873-12677971 -	AA-------CTAT---CTCGGAA-CTATTATACGATAAACTTTGTTCAGTTTTGCCA----------------ACTTGCT------T--GCAGCACTGGGTGAAGTTTGTCTTATAATCCCACAGTAA--------G-----C-GATC
droPer1	super_2:8905688-8905793 +	AAAAACGAACTATCTCGGA---A-CTATTATACGATAAACTTTGTTCAGTTTTGCCA----------------ACTTGCT------T--GCAGCACTGGGTGAAGTTTGTCTTATAATCCCACAGTAA--------G-----C-GATC
droWil1	scaffold_180701:1652063-1652174 -	A---------TTT---CATATAA-T-ATTATATGATAAACTTTGTTCAGTTTTGCAATCAAAAAATCAAGAAAA-TTCCT------TA-GCATCACTGGGTGAAGTTTGTCTTATAATCTTTAAACTA--------------C-AATC
droVir3	scaffold_12875:4111798-4111897 +	TA---------------TAACAA-ATATTATATGGTAAACTTTGTTCAGTTTTGCCA----------------GGCTCTT-TTTGTGC-GCATCACTGGGTGAAGTTTGTCGTATAATCGCAAAAAGA--------------A-CAGC
droMoj3	scaffold_6496:20937092-20937209 -	TA-------TACA---AGAACAGAATATTATAAGGTAAACTTTGTTCAGTTTTGCCG----------------GGTTCTATGTTGTGC-GCATCACTGGGTGAAGTTTGTCGTATAATCG--AAACGAAACAGCAAGGAAAAA-GTCC
droGri2	scaffold_15112:2109131-2109231 -	AA-------ATAT---ATAATTA-C-ATTATATGGCAAACCTTGTTCAGTTTTGACA----------------ACTTTGTTTCTGTGCATCATCACTGGGTGAAGTTTGTCGTATAATC--GAAATG-----------------GATC