(5)
OTHER.mut
(6)
PIWI.ip
(2)
PIWI.mut
(27)
TESTES

Sense strand
CTTCCCTACAGCAGGCGTCATTGAAACTCTTCGAAATGGCGTACAAAAAGGTACAAGGGCTGGAGGGGTAACTGGGACCTAGTTATTGGGGTTCCCTCTACCATTTATAGAATTGAGAAAACTGTAAATAGAATGTCACGGGGAAGATGAGGTTTGTACGGTGAATGAAGCCTGTTGAACAGAGAGCGAACTTGTGGATACCTGTGAAATCTTAGTGATCCTCAAGTATTTTCTCAACAGATGGCATCTGAACGGGAAGGTTCTGGAAGTTCTGGCACTGGGGAACAGAA
..................................................................((((...(((((((........)))))))...)))).(((((((...........))))))).......(((((((.......((((((((..(((.......))).((...)).....)))))))).......)))))))...................................................................................
................................................................65.............................................................................................................................................208................................................................................
Size Perfect hit Total Norm Perfect Norm SRR363964(GSM822766)
AdultGlobal 5'-RACEread_length: 105. (testes)
SRR034119(GSM466729)
Mili IP_Tdrd9+/- replicate2. (mili testes)
SRR069809(GSM610965)
small RNA sequencing; sample 1. (testes)
SRR034118(GSM466728)
Mili IP_Tdrd9+/- replicate1. (mili testes)
SRR248527(GSM733815)
cell type: spermatogonial stem cell enriched . (testes)
SRR248524(GSM733812)
cell type: Thy1+ spermatogonial stem cellstra. (testes)
SRR028732(GSM400969)
Mili-Tdrd1 KO associated. (mili testes)
SRR248523(GSM733811)
cell type: Thy1+ spermatogonial stem cellstra. (testes)
mjTestesWT1()
Testes Data. (testes)
mjTestesKO7()
Testes Data. (Zcchc11 testes)
SRR248525(GSM733813)
cell type: Thy1- spermatogonial stem cellstra. (testes)
mjTestesWT3()
Testes Data. (testes)
mjTestesKO8()
Testes Data. (Zcchc11 testes)
SRR069810(GSM610966)
small RNA sequencing; sample 2. (testes)
SRR248526(GSM733814)
cell type: Thy1- spermatogonial stem cellstra. (testes)
GSM509279(GSM509279)
MVH-/- E16.5 small RNA. (testes)
SRR034121(GSM466731)
Mili IP_Tdrd9-/- replicate2. (mili testes)
SRR034120(GSM466730)
Mili IP_Tdrd9-/- replicate1. (mili testes)
GSM475280(GSM475280)
Mili-IP. (mili testes)
GSM475281(GSM475281)
total RNA. (testes)
GSM509277(GSM509277)
small RNA cloning by length. (piwi testes)
GSM179088(GSM179088)
Developmentally regulated piRNA clusters implicate MILI in transposon control. (piwi testes)
SRR014231(GSM319955)
16.5 dpc total. (testes)
SRR029036(GSM433288)
18dpp_hetero_tdrd6-KO. (tdrd6 testes)
SRR029037(GSM433289)
18dpp_homo_tdrd6-KO. (tdrd6 testes)
SRR029038(GSM433290)
25dpp_hetero_tdrd6-KO. (tdrd6 testes)
SRR069811(GSM610967)
small RNA sequencing; sample 3. (testes)
.....................................................................AACTGGGACCTAGTTATTGGGGTTCCCTCTACCATTTATAGAATTGAGAAAA......................................................................................................................................................................... 52 1 59.00 59.00 59.00 - - - - - - - - - - - - - - - - - - - - - - - - - -
........................................................................................................................................................................................................................................................TGAACGGGAAGGTTCTGGAAGTTCTGGC.............. 28 2 7.00 7.00 - 3.00 - 0.50 - 0.50 1.00 - - - - - - - - - 1.00 1.00 - - - - - - - - -
.....................TGAAACTCTTCGAAATGGCGTACAAAAA................................................................................................................................................................................................................................................. 28 1 7.00 7.00 - - - - 1.00 - - 1.00 - - 5.00 - - - - - - - - - - - - - - - -
.....................TGAAACTCTTCGAAATGGCGTACAAAA.................................................................................................................................................................................................................................................. 27 1 5.00 5.00 - - - 1.00 1.00 - - 2.00 - - - - - - - - - - - 1.00 - - - - - - -
...................................................................................................................................................................................................................................................................GTTCTGGAAGTTCTGGCACTGGGGAAC.... 27 1 5.00 5.00 - - - - - - - - 5.00 - - - - - - - - - - - - - - - - - -
....................TTGAAACTCTTCGAAATGGCGTACAAAA.................................................................................................................................................................................................................................................. 28 1 5.00 5.00 - 2.00 - 1.00 - 1.00 1.00 - - - - - - - - - - - - - - - - - - - -
.....................TGAAACTCTTCGAAATGGCGTACAAAAAG................................................................................................................................................................................................................................................ 29 1 4.00 4.00 - - - 1.00 2.00 - - - - - - - - - - - - - - 1.00 - - - - - - -
........................................................................................................................................................................AGCCTGTTGAACAGAGAGCGA..................................................................................................... 21 1 3.00 3.00 - - - - - - - - - - - 3.00 - - - - - - - - - - - - - - -
.......................................................................................................................................................................................................................................................................TGGAAGTTCTGGCACTGGGGAACAGA. 26 1 3.00 3.00 - - 2.00 - - 1.00 - - - - - - - - - - - - - - - - - - - - -
................................................................................................................................................................................................................................................ATGGCATCTGAACGGGAA................................ 18 1 3.00 3.00 - - - - - - - - 3.00 - - - - - - - - - - - - - - - - - -
.....................TGAAACTCTTCGAAATGGCGTACAAAAAGa............................................................................................................................................................................................................................................... 30 a 3.00 4.00 - - - - 2.00 1.00 - - - - - - - - - - - - - - - - - - - - -
.................................................................................................................................................................................................................................................TGGCATCTGAACGGGAAGGTTCTGGA....................... 26 2 2.50 2.50 - 0.50 1.50 - - - 0.50 - - - - - - - - - - - - - - - - - - - -
......TACAGCAGGCGTCATTGAAACTCTTC.................................................................................................................................................................................................................................................................. 26 1 2.00 2.00 - - - 1.00 - - - - - - - - - - - - - - - - - 1.00 - - - - -
....................TTGAAACTCTTCGAAATGGCGTACAAAAA................................................................................................................................................................................................................................................. 29 1 2.00 2.00 - - - - 1.00 - - - - - - - - - - - 1.00 - - - - - - - - - -
...................ATTGAAACTCTTCGAAATGGCGTACAAAAA................................................................................................................................................................................................................................................. 30 1 2.00 2.00 - - - - 1.00 - - - - 1.00 - - - - - - - - - - - - - - - - -
.....................TGAAACTCTTCGAAATGGCGTACAAA................................................................................................................................................................................................................................................... 26 1 2.00 2.00 - - - - - - - - - - 2.00 - - - - - - - - - - - - - - - -
...................ATTGAAACTCTTCGAAATGGCGTAC...................................................................................................................................................................................................................................................... 25 1 2.00 2.00 - - - - - 2.00 - - - - - - - - - - - - - - - - - - - - -
......................GAAACTCTTCGAAATGGCGTACAAAA.................................................................................................................................................................................................................................................. 26 1 2.00 2.00 - 1.00 1.00 - - - - - - - - - - - - - - - - - - - - - - - -
......................GAAACTCTTCGAAATGGCGTAC...................................................................................................................................................................................................................................................... 22 1 2.00 2.00 - - - - - - - - - - - - 2.00 - - - - - - - - - - - - - -
.....................TGAAACTCTTCGAAATGGCG......................................................................................................................................................................................................................................................... 20 1 2.00 2.00 - - 2.00 - - - - - - - - - - - - - - - - - - - - - - - -
........................................................................................................................................................................................................................................................TGAACGGGAAGGTTCTGGAAGTTCTGG............... 27 2 1.50 1.50 - - 0.50 0.50 - - 0.50 - - - - - - - - - - - - - - - - - - - -
...............................................................................................................................................................................................................................................GATGGCATCTGAACGGGAAGG.............................. 21 1 1.00 1.00 - - - - - - - - - - - - - - - 1.00 - - - - - - - - - - -
.........AGCAGGCGTCATTGAAACTCTTCGAA............................................................................................................................................................................................................................................................... 26 1 1.00 1.00 - 1.00 - - - - - - - - - - - - - - - - - - - - - - - - -
..................................................GTACAAGGGCTGGAGGGGTAACTGGGAC.................................................................................................................................................................................................................... 28 1 1.00 1.00 - - - - - - - - - - - - - - - - - - 1.00 - - - - - - - -
.......ACAGCAGGCGTCATTGAAACTCTTCGAAA.............................................................................................................................................................................................................................................................. 29 1 1.00 1.00 - - - 1.00 - - - - - - - - - - - - - - - - - - - - - - -
.................TCATTGAAACTCTTCGAAATGGCGTACA..................................................................................................................................................................................................................................................... 28 1 1.00 1.00 - - - - - - - - - 1.00 - - - - - - - - - - - - - - - - -
........................................................................................................................................................................................................................................................TGAACGGGAAGGTTCTGGAAGTTC.................. 24 2 1.00 1.00 - 0.50 - - - - - - - - - - - - - - - - - - - - - - - 0.50 -
...........................................................................................................................................................................................................................................................................AGTTCTGGCACTGGGGAACAGA. 22 1 1.00 1.00 - - - - - - - - - - - 1.00 - - - - - - - - - - - - - - -
.....................................................................................................................................................................................................................................................................TCTGGAAGTTCTGGCACTGG......... 20 1 1.00 1.00 - - 1.00 - - - - - - - - - - - - - - - - - - - - - - - -
..TCCCTACAGCAGGCGTCATTGAAACTC..................................................................................................................................................................................................................................................................... 27 1 1.00 1.00 - - - 1.00 - - - - - - - - - - - - - - - - - - - - - - -
................................................................................................................................................................................................................................................ATGGCATCTGAACGGGAAGGTTCTGGAA...................... 28 1 1.00 1.00 - - - - - - - - - - - - - - - - - - - - 1.00 - - - - - -
.....................TGAAACTCTTCGAAATGGCGT........................................................................................................................................................................................................................................................ 21 1 1.00 1.00 - - - - - - - - - - - 1.00 - - - - - - - - - - - - - - -
.................TCATTGAAACTCTTCGAAATGGCGTAC...................................................................................................................................................................................................................................................... 27 1 1.00 1.00 - - - - - - - 1.00 - - - - - - - - - - - - - - - - - - -
.......ACAGCAGGCGTCATTGAAACTCTTCGAA............................................................................................................................................................................................................................................................... 28 1 1.00 1.00 - - - - - - - - - - - - - - - 1.00 - - - - - - - - - - -
......TACAGCAGGCGTCATTGAAACTCTTCGA................................................................................................................................................................................................................................................................ 28 1 1.00 1.00 - - - - - - - - - - - - - - 1.00 - - - - - - - - - - - -
....................TTGAAACTCTTCGAAATGGCGTACAA.................................................................................................................................................................................................................................................... 26 1 1.00 1.00 - - - - - - - 1.00 - - - - - - - - - - - - - - - - - - -
..................................................GTACAAGGGCTGGAGGGGTAACTGaa...................................................................................................................................................................................................................... 26 aa 1.00 0.00 - - - - - 1.00 - - - - - - - - - - - - - - - - - - - - -
...................ATTGAAACTCTTCGAAATGGCGTACA..................................................................................................................................................................................................................................................... 26 1 1.00 1.00 - - - - - - - - - - - - - - 1.00 - - - - - - - - - - - -
...........CAGGCGTCATTGAAACTCTTCGAAATGt........................................................................................................................................................................................................................................................... 28 t 1.00 0.00 - - - - - - 1.00 - - - - - - - - - - - - - - - - - - - -
.................................................................................................................................................................................................................................................................AGGTTCTGGAAGTTCTGGCACTGGGGAAC.... 29 1 1.00 1.00 - - - - - - - - - 1.00 - - - - - - - - - - - - - - - - -
..................................................................................................................................................................................................................................................GGCATCTGAACGGGAAGGTTCTGGAA...................... 26 2 1.00 1.00 - - - - - 0.50 - - - - - - - - - - - - - - - - - - - - 0.50
....................TTGAAACTCTTCGAAATGGCGTACA..................................................................................................................................................................................................................................................... 25 1 1.00 1.00 - - - - - 1.00 - - - - - - - - - - - - - - - - - - - - -
.................................................................................................................................................................................................................................................................AGGTTCTGGAAGTTCTGGCACTGGGGA...... 27 1 1.00 1.00 - - - - - - - - - - - - - - - - - 1.00 - - - - - - - - -
........................................................................................................................................................................................................................................................................GGAAGTTCTGGCACTGGGGAACAGcag 27 cag 1.00 0.00 - - - - - - - - - - - - - 1.00 - - - - - - - - - - - - -
..................CATTGAAACTCTTCGAAATGGCGTAC...................................................................................................................................................................................................................................................... 26 1 1.00 1.00 - - - - - - - - - - - 1.00 - - - - - - - - - - - - - - -
..................CATTGAAACTCTTCGAAATGGCGTACA..................................................................................................................................................................................................................................................... 27 1 1.00 1.00 - - - - - - - - - 1.00 - - - - - - - - - - - - - - - - -
.....................TGAAACTCTTCGAAATGGCGTA....................................................................................................................................................................................................................................................... 22 1 1.00 1.00 - - 1.00 - - - - - - - - - - - - - - - - - - - - - - - -
....................TTGAAACTCTTCGAAATGGCGTACAAAAt................................................................................................................................................................................................................................................. 29 t 1.00 5.00 - - - - - - 1.00 - - - - - - - - - - - - - - - - - - - -
..TCCCTACAGCAGGCGTCATTGAAACTCT.................................................................................................................................................................................................................................................................... 28 1 1.00 1.00 - 1.00 - - - - - - - - - - - - - - - - - - - - - - - - -
........CAGCAGGCGTCATTGAAACTCTTCGA................................................................................................................................................................................................................................................................ 26 1 1.00 1.00 - 1.00 - - - - - - - - - - - - - - - - - - - - - - - - -
..........GCAGGCGTCATTGAAACTCTTCG................................................................................................................................................................................................................................................................. 23 1 1.00 1.00 - - - - - - - - - - - - - - - - - - 1.00 - - - - - - - -
.....................TGAAACTCTTCGAAATGGCGTACAAAAt................................................................................................................................................................................................................................................. 28 t 1.00 5.00 - - - - - - - - - - - - - 1.00 - - - - - - - - - - - - -
....................TTGAAACTCTTCGAAATGGCGT........................................................................................................................................................................................................................................................ 22 1 1.00 1.00 - - - - 1.00 - - - - - - - - - - - - - - - - - - - - - -
....................TTGAAACTCTTCGAAATGGCGTACAAAAAGa............................................................................................................................................................................................................................................... 31 a 1.00 0.00 - - - - - - 1.00 - - - - - - - - - - - - - - - - - - - -
......................................................................................................................................................................................................................................................TCTGAACGGGAAGGTTCTGGAAGTT................... 25 2 1.00 1.00 - 1.00 - - - - - - - - - - - - - - - - - - - - - - - - -
........................................................................................................................................................................................................................................................TGAACGGGAAGGTTCTGGAAGTTCTGGt.............. 28 t 1.00 1.50 - - - - - - 0.50 - - - - - - 0.50 - - - - - - - - - - - - -
...................................................................................................................................................................AATGAAGCCTGTTGAACAGAGAGCGAAC................................................................................................... 28 1 1.00 1.00 - - - - - - - - - 1.00 - - - - - - - - - - - - - - - - -
........................................................................................................................................................................................................................................................TGAACGGGAAGGTTCTGGAAGTTCTG................ 26 2 1.00 1.00 - 0.50 - - - - - - - - - - - - - 0.50 - - - - - - - - - - -
........................................................................................................................................................................................................................................................................GGAAGTTCTGGCACTGGGG....... 19 1 1.00 1.00 - - - - - - - 1.00 - - - - - - - - - - - - - - - - - - -
....CCTACAGCAGGCGTCATTGA.......................................................................................................................................................................................................................................................................... 20 1 1.00 1.00 - - 1.00 - - - - - - - - - - - - - - - - - - - - - - - -
..............................................................................................................................................................................................................................................AGATGGCATCTGAACGGGAAGGTTCTG......................... 27 1 1.00 1.00 - - - - - - - - - - - - - - - - - - - - - - 1.00 - - - -
...CCCTACAGCAGGCGTCATTGAAACTCT.................................................................................................................................................................................................................................................................... 27 1 1.00 1.00 - - - - - - 1.00 - - - - - - - - - - - - - - - - - - - -
......TACAGCAGGCGTCATTGAAACTCTT................................................................................................................................................................................................................................................................... 25 1 1.00 1.00 - - - - - - - - - - - - - - - - - - - - - - - 1.00 - - -
.....................................................................................................................................................................................................................................................ATCTGAACGGGAAGGTT............................ 17 2 1.00 1.00 - - - - - - - - - 1.00 - - - - - - - - - - - - - - - - -
...................................................................................................................................................................................CAGAGAGCGAACTTGTGGATACCTG...................................................................................... 25 1 1.00 1.00 - - - - - - - 1.00 - - - - - - - - - - - - - - - - - - -
.TTCCCTACAGCAGGCGTCATTGAAACTC..................................................................................................................................................................................................................................................................... 28 1 1.00 1.00 - 1.00 - - - - - - - - - - - - - - - - - - - - - - - - -
.......................................................................................................................................................................................................................................................CTGAACGGGAAGGTTCTGGAAGTTCT................. 26 2 0.50 0.50 - - - - - - 0.50 - - - - - - - - - - - - - - - - - - - -
......................................................................................................................................................................................................................................................TCTGAACGGGAAGGTTCTGGAAGTTCTGGCAC............ 32 2 0.50 0.50 - - - - - - - - - - - - - - 0.50 - - - - - - - - - - - -
............................................................................................................................................................................................................................................................CGGGAAGGTTCTGGAAGTTCTGG............... 23 2 0.50 0.50 - - - - - - - - - - - - 0.50 - - - - - - - - - - - - - -
........................................................................................................................................................................................................................................................TGAACGGGAAGGTTCTGGAAGTTCT................. 25 2 0.50 0.50 - - 0.50 - - - - - - - - - - - - - - - - - - - - - - - -
.................................................................................................................................................................................................................................................TGGCATCTGAACGGGAAGGTTCTGG........................ 25 2 0.50 0.50 - - - 0.50 - - - - - - - - - - - - - - - - - - - - - - -
.................................................................................................................................................................................................................................................TGGCATCTGAACGGGAAGGTTCTGGAA...................... 27 2 0.50 0.50 - - - - - - - 0.50 - - - - - - - - - - - - - - - - - - -
.................................................................................................................................................................................................................................................TGGCATCTGAACGGGAAGGT............................. 20 2 0.50 0.50 - - - - - - - 0.50 - - - - - - - - - - - - - - - - - - -
........................................................................................................................................................................................................................................................TGAACGGGAAGGTTCTGGAAGTTCTGGCt............. 29 t 0.50 7.00 - - - - - - - - - - - - - 0.50 - - - - - - - - - - - - -
......................................................................................................................................................................................................................................................TCTGAACGGGAAGGTTCTGGAAGTTCT................. 27 2 0.50 0.50 - - - 0.50 - - - - - - - - - - - - - - - - - - - - - - -
................................................................................................................................................................................................................................................................AAGGTTCTGGAAGTTCTGGCA............. 21 2 0.50 0.50 - - - - - - - - - - - - 0.50 - - - - - - - - - - - - - -
..........................................................................................................................................................................................................................................................AACGGGAAGGTTCTGGAAGTTCTGGC.............. 26 2 0.50 0.50 - - - - - - - - - - - - - - - - - - - - - - - - 0.50 - -
........................................................................................................................................................................................................................................................TGAACGGGAAGGTTCTGGAAGTTCTtgc.............. 28 tgc 0.50 0.50 - - - 0.50 - - - - - - - - - - - - - - - - - - - - - - -
..........................................................................................................................................................................................................................................................AACGGGAAGGTTCTGGAAGTTC.................. 22 2 0.50 0.50 - - 0.50 - - - - - - - - - - - - - - - - - - - - - - - -
..................................................................................................................................................................................................................................................GGCATCTGAACGGGAAGGTTCTGGAAG..................... 27 2 0.50 0.50 - 0.50 - - - - - - - - - - - - - - - - - - - - - - - - -
........................................................................................................................................................................................................................................................TGAACGGGAAGGTTCTGGAAGTTCTGc............... 27 c 0.50 1.00 - 0.50 - - - - - - - - - - - - - - - - - - - - - - - - -
..................................................................................................................................................................................................................................................GGCATCTGAACGGGAAGGTTCT.......................... 22 2 0.50 0.50 - - 0.50 - - - - - - - - - - - - - - - - - - - - - - - -
........................................................................................................................................................................................................................................................TGAACGGGAAGGTTCTGGAAGTTCTGtt.............. 28 tt 0.50 1.00 - 0.50 - - - - - - - - - - - - - - - - - - - - - - - - -
........................................................................................................................................................................................................................................................TGAACGGGAAGGTTCTGGAAGTTCTGt............... 27 t 0.50 1.00 - - - 0.50 - - - - - - - - - - - - - - - - - - - - - - -

Antisense strand
CTTCCCTACAGCAGGCGTCATTGAAACTCTTCGAAATGGCGTACAAAAAGGTACAAGGGCTGGAGGGGTAACTGGGACCTAGTTATTGGGGTTCCCTCTACCATTTATAGAATTGAGAAAACTGTAAATAGAATGTCACGGGGAAGATGAGGTTTGTACGGTGAATGAAGCCTGTTGAACAGAGAGCGAACTTGTGGATACCTGTGAAATCTTAGTGATCCTCAAGTATTTTCTCAACAGATGGCATCTGAACGGGAAGGTTCTGGAAGTTCTGGCACTGGGGAACAGAA
..................................................................((((...(((((((........)))))))...)))).(((((((...........))))))).......(((((((.......((((((((..(((.......))).((...)).....)))))))).......)))))))...................................................................................
................................................................65.............................................................................................................................................208................................................................................
Size Perfect hit Total Norm Perfect Norm SRR363964(GSM822766)
AdultGlobal 5'-RACEread_length: 105. (testes)
SRR034119(GSM466729)
Mili IP_Tdrd9+/- replicate2. (mili testes)
SRR069809(GSM610965)
small RNA sequencing; sample 1. (testes)
SRR034118(GSM466728)
Mili IP_Tdrd9+/- replicate1. (mili testes)
SRR248527(GSM733815)
cell type: spermatogonial stem cell enriched . (testes)
SRR248524(GSM733812)
cell type: Thy1+ spermatogonial stem cellstra. (testes)
SRR028732(GSM400969)
Mili-Tdrd1 KO associated. (mili testes)
SRR248523(GSM733811)
cell type: Thy1+ spermatogonial stem cellstra. (testes)
mjTestesWT1()
Testes Data. (testes)
mjTestesKO7()
Testes Data. (Zcchc11 testes)
SRR248525(GSM733813)
cell type: Thy1- spermatogonial stem cellstra. (testes)
mjTestesWT3()
Testes Data. (testes)
mjTestesKO8()
Testes Data. (Zcchc11 testes)
SRR069810(GSM610966)
small RNA sequencing; sample 2. (testes)
SRR248526(GSM733814)
cell type: Thy1- spermatogonial stem cellstra. (testes)
GSM509279(GSM509279)
MVH-/- E16.5 small RNA. (testes)
SRR034121(GSM466731)
Mili IP_Tdrd9-/- replicate2. (mili testes)
SRR034120(GSM466730)
Mili IP_Tdrd9-/- replicate1. (mili testes)
GSM475280(GSM475280)
Mili-IP. (mili testes)
GSM475281(GSM475281)
total RNA. (testes)
GSM509277(GSM509277)
small RNA cloning by length. (piwi testes)
GSM179088(GSM179088)
Developmentally regulated piRNA clusters implicate MILI in transposon control. (piwi testes)
SRR014231(GSM319955)
16.5 dpc total. (testes)
SRR029036(GSM433288)
18dpp_hetero_tdrd6-KO. (tdrd6 testes)
SRR029037(GSM433289)
18dpp_homo_tdrd6-KO. (tdrd6 testes)
SRR029038(GSM433290)
25dpp_hetero_tdrd6-KO. (tdrd6 testes)
SRR069811(GSM610967)
small RNA sequencing; sample 3. (testes)
...................................................................................................................................................................................................................................................................CTGGAAGTTCTGGCACTgtt........... 20 gtt 1.00 0.00 - - - - - - - - - 1.00 - - - - - - - - - - - - - - - - -