ID: mmu-mir-6961
GENE: BC141885
chr15:100479544-100479793-


(1)
AGO1.ip 		(3)
AGO2.ip 		(11)
B-CELL 		(11)
BRAIN 		(2)
CELL-LINE 		(1)
DCR.mut 		(7)
EMBRYO 		(5)
ESC 		(1)
KIDNEY 		(7)
LIVER 		(1)
LUNG 		(5)
LYMPH 		(19)
OTHER 		(1)
OTHER.ip 		(5)
OTHER.mut 		(5)
PIWI.ip 		(3)
PIWI.mut 		(13)
SPLEEN 		(1)
TDRD1.ip 		(27)
TESTES 		(3)
THYMUS

Sense strand
CCTGAGGGAAACCCTACCCTGAGAGAGACCCTGCCCTGAGTGAGCTACCTTGAGGGAGGGGGGGGGGGGGAGGAAGGGAACAATGGACCAAGGAGTAAAAGAGCAGGGTGGGCCAGAGGAGGTTTTGATTGGGAAAGCTGACTGGGAAGGAAATGGCCAGGGCTCTCTAACTAACCTGTCCTCTTTCTTCCTGGCTCTAGTTGAACCACAATCTCTACGAAGTGATGAGCAAACTGGAGAAACAACATTC
...........................................................................................................................................(((((((((((((..((.((((.............)))).))..))))))))))).)).....................................................
.....................................................................................................................................134...............................................................200................................................
SizePerfect hitTotal NormPerfect NormSRR042471(GSM539863)
mouse TFHS cells [09-002]. (spleen)		SRR059769(GSM562831)
Treg_Drosha. (spleen)		SRR042458(GSM539850)
mouse basophil cells [09-002]. (b cell)		mjLiverKO1()
Liver Data. (Zcchc11 liver)		SRR042459(GSM539851)
mouse neutrophil cells replicate 1 [09-002]. (blood)		SRR042470(GSM539862)
mouse Th17 cells [09-002]. (lymph)		SRR028732(GSM400969)
Mili-Tdrd1 KO associated. (mili testes)		SRR042456(GSM539848)
mouse hematopoetic progenitor cells [09-002]. (bone marrow)		SRR042469(GSM539861)
mouse Th2 cells [09-002]. (lymph)		SRR042472(GSM539864)
mouse iTreg cells [09-002]. (lymph)		SRR345200(SRX097261)
source: size fractionated RNA from mouse hipp. (brain)		GSM519104(GSM519104)
mouse_granulocyte_nuclei_smallRNAs. (cell line)		SRR059770(GSM562832)
Treg_Dicer. (spleen)		SRR042465(GSM539857)
mouse CD4+ T cells (spleen) [09-002]. (spleen)		SRR042466(GSM539858)
mouse CD8+ T cells (spleen) [09-002]. (spleen)		SRR042450(GSM539842)
mouse B cells activated in vitro with LPS/IL4 replicate 3 [09-002]. (b cell)		SRR042484(GSM539876)
mouse salivary gland tissue [09-002]. (gland)		SRR034118(GSM466728)
Mili IP_Tdrd9+/- replicate1. (mili testes)		GSM475280(GSM475280)
Mili-IP. (mili testes)		SRR042447(GSM539839)
mouse mature B cells (spleen) replicate 2 [09-002]. (b cell)		SRR042488(GSM539880)
mouse BclXL germinal center B cells [BalbC] [09-002]. (B cell)		mjLiverWT1()
Liver Data. (liver)		SRR042463(GSM539855)
mouse natural killer cells [09-002]. (spleen)		SRR059766(GSM562828)
DN3_Drosha. (thymus)		SRR345196(SRX097257)
source: size fractionated RNA from mouse hipp. (brain)		SRR042449(GSM539841)
mouse B cells activated in vitro with LPS/IL4 replicate 2 [09-002]. (b cell)		SRR042448(GSM539840)
mouse B cells activated in vitro with LPS/IL4 replicate 1 [09-002]. (b cell)		SRR059772(GSM562834)
CD4_Drosha. (spleen)		SRR525244(SRA056111/SRX170320)
Mus musculus domesticus miRNA sequencing. (ago2 Blood)		SRR042455(GSM539847)
mouse plasma cells [09-002]. (blood)		SRR042487(GSM539879)
mouse BclXL B activated with LPS/IL4 [BalbC] [09-002]. (spleen)		SRR345198(SRX097259)
source: size fractionated RNA from mouse hipp. (brain)		SRR346414(SRX098255)
Global profiling of miRNA and the hairpin pre. (Spleen)		SRR042473(GSM539865)
mouse nTreg cells [09-002]. (lymph)		SRR042483(GSM539875)
mouse muscle tissue [09-002]. (muscle)		SRR034120(GSM466730)
Mili IP_Tdrd9-/- replicate1. (mili testes)		SRR042462(GSM539854)
mouse macrophages [09-002]. (bone marrow)		SRR248523(GSM733811)
cell type: Thy1+ spermatogonial stem cellstra. (testes)		SRR042457(GSM539849)
mouse mast cells [09-002]. (bone marrow)		GSM509275(GSM509275)
MitoPLD+/+ E16.5 small RNA. (testes)		SRR525242(SRA056111/SRX170318)
Mus musculus domesticus miRNA sequencing. (ago2 Blood)		SRR059771(GSM562833)
CD4_control. (spleen)		SRR042461(GSM539853)
mouse dendritic cells [09-002]. (bone marrow)		GSM509279(GSM509279)
MVH-/- E16.5 small RNA. (testes)		SRR248524(GSM733812)
cell type: Thy1+ spermatogonial stem cellstra. (testes)		SRR029037(GSM433289)
18dpp_homo_tdrd6-KO. (tdrd6 testes)		mjTestesKO7()
Testes Data. (Zcchc11 testes)		SRR042467(GSM539859)
mouse T cells activated in vitro with Concanavalin A [09-002]. (spleen)		SRR345199(SRX097260)
source: size fractionated RNA from mouse hipp. (brain)		SRR042468(GSM539860)
mouse Th1 cells [09-002]. (lymph)		SRR345203(SRX097264)
source: size fractionated RNA from mouse hipp. (brain)		mjTestesWT3()
Testes Data. (testes)		SRR029038(GSM433290)
25dpp_hetero_tdrd6-KO. (tdrd6 testes)		SRR037901(GSM510437)
testes_rep2. (testes)		SRR525238(SRA056111/SRX170314)
Second IgG control. (Blood)		SRR073954(GSM629280)
total RNA. (blood)		GSM509277(GSM509277)
small RNA cloning by length. (piwi testes)		SRR034119(GSM466729)
Mili IP_Tdrd9+/- replicate2. (mili testes)		SRR028730(GSM400967)
Tdrd1-associated. (tdrd1 testes)		mjLiverKO3()
Liver Data. (Zcchc11 liver)		SRR059765(GSM562827)
DN3_control. (thymus)		SRR346419(SRX098260)
Global profiling of miRNA and the hairpin pre. (Lung)		GSM314557(GSM314557)
ESC dgcr8 (Illumina). (ESC)		SRR042460(GSM539852)
mouse neutrophil cells replicate 2 [09-002]. (blood)		SRR037929(GSM510467)
e7p5_rep3. (embryo)		SRR346425(SRX098267)
Global profiling of miRNA and the hairpin pre. (Neuroblastoma)		SRR391845(GSM852140)
gender: femalegenotype/variation: wild typeti. (embryo)		SRR051940(GSM545784)
18-32 nt total small RNAs (Mov10l+/-). (testes)		GSM510444(GSM510444)
brain_rep5. (brain)		SRR042454(GSM539846)
mouse germinal center B cells (lymph node) [09-002]. (b cell)		SRR065054(SRR065054)
Tissue-specific Regulation of Mouse MicroRNA . (jejunum)		SRR391846(GSM852141)
gender: femalegenotype/variation: ADAR2-/-tis. (embryo)		GSM314553(GSM314553)
ESC dcr (Illumina). (ESC)		SRR042452(GSM539844)
mouse B1 B cells [09-002]. (b cell)		SRR034121(GSM466731)
Mili IP_Tdrd9-/- replicate2. (mili testes)		SRR553602(SRX182808)
source: Sertoli cells. (Sertoli cells)		GSM509278(GSM509278)
small RNA cloning by length. (piwi testes)		SRR038743(GSM527278)
small RNA-Seq. (dicer brain)		SRR391853(GSM852148)
gender: femalegenotype/variation: ADAR2-/-/AD. (embryo)		SRR402762(SRX117945)
source: ES E14 male cells. (ESC)		SRR028731(GSM400968)
Mili-wt-associated. (testes)		SRR525237(SRA056111/SRX170313)
Mus musculus domesticus miRNA sequencing. (Blood)		SRR042480(GSM539872)
mouse kidney tissue [09-002]. (kidney)		SRR023852(GSM307161)
ZHBT-c424hsmallrna_rep1. (cell line)		SRR391851(GSM852146)
gender: femalegenotype/variation: wild typeti. (embryo)		GSM640578(GSM640578)
small RNA in the liver with paternal control. (liver)		SRR248527(GSM733815)
cell type: spermatogonial stem cell enriched . (testes)		SRR345207(SRX097268)
source: size fractionated RNA from mouse hipp. (brain)		SRR042464(GSM539856)
mouse CD4+CD8+ T cells (thymus) [09-002]. (thymus)		SRR525240(SRA056111/SRX170316)
Mus musculus domesticus miRNA sequencing. (ago1 Blood)		SRR345205(SRX097266)
source: size fractionated RNA from mouse hipp. (brain)		SRR065055(SRR065055)
Tissue-specific Regulation of Mouse MicroRNA . (jejunum)		SRR042443(GSM539835)
mouse pro B cells [09-002]. (b cell)		SRR069811(GSM610967)
small RNA sequencing; sample 3. (testes)		SRR391852(GSM852147)
gender: femalegenotype/variation: ADAR2-/-tis. (embryo)		SRR306536(GSM750579)
19-24nt. (ago2 brain)		SRR279904(GSM689054)
cell type: mouse embryonic stem cellcell line. (ESC)		SRR040488(GSM533911)
E18.5 wild type LIVER small RNA. (liver)		SRR059768(GSM562830)
Treg_control. (spleen)		SRR051939(GSM545783)
Mov10L1-associated piRNAs. (mov10L testes)		SRR059773(GSM562835)
CD4_Dicer. (spleen)		mjLiverWT3()
Liver Data. (liver)		SRR014231(GSM319955)
16.5 dpc total. (testes)		SRR345201(SRX097262)
source: size fractionated RNA from mouse hipp. (brain)		SRR346415(SRX098256)
source: Testis. (Testes)		SRR042445(GSM539837)
mouse immature B cells [09-002]. (b cell)		SRR363956(GSM822758)
P14-WTSmall RNA Miwi IPread_length: 36. (testes)		SRR037902(GSM510438)
testes_rep3. (testes)		SRR077866(GSM637803)
18-30 nt small RNAs. (liver)		SRR037924(GSM510462)
e9p5_rep2. (embryo)		SRR042474(GSM539866)
mouse embryonic stem cells [09-002]. (ESC)		SRR042446(GSM539838)
mouse mature B cells (spleen) replicate 1 [09-002]. (b cell)
..........................................................................................................................................TGACTGGGAAGGAAATGGCC............................................................................................20126.0026.002.00-2.00-5.002.00--1.00----1.001.002.001.00--1.00--------1.00----1.001.00--------------1.00---------2.00---1.00----------------------------------------------1.00-
..........................................................................................................................................TGACTGGGAAGGAAATGGCCA...........................................................................................21114.0014.001.00---1.001.00--1.001.00----1.00----1.001.005.00------------------------------------------------------------1.00-----------------------------
.......................................................................................................................................................................................................................TACGAAGTGATGAGCAAACTGGAGAA.........26114.0014.00------5.00----------------------------1.00---1.00---1.00------------1.001.001.00-----------------1.00----------------------1.00-------1.00----
.........................................................................................................................................CTGACTGGGAAGGAAATGGCCA...........................................................................................22113.0013.006.00-2.00----------1.001.001.00---------1.00--------1.00-----------------------------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAG..........................................................................................22112.0012.00---8.00------------1.00-----1.001.00----------------------------------------------------------------------1.00-----------------
..................................................................................................................................................................................TCCTCTTTCTTCCTGGCTCTAGT.................................................23110.0010.00-4.00---------1.00-----------1.00------------------------------------1.001.00-----------------------1.00-----------------------1.00--
..........................................................................................................................................TGACTGGGAAGGAAATGGC.............................................................................................1919.009.001.00-2.00-1.003.00---1.00------1.00-----------------------------------------------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAGA.........................................................................................2319.0012.001.00------1.00---1.00----------2.00---------1.00-----------------------------------1.00-----------------------------1.00--1.00----------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAGT.........................................................................................2318.0012.00-6.00------------------1.00-----------------1.00-------------------------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAAT.........................................................................................2318.0014.002.00---1.00---1.00-----------------------------1.00---1.00--------------------1.00----------------------------1.00-------------------
.......................................................................................................................................................................................................................TACGAAGTGATGAGCAAACTGGAGAAAC.......2817.007.00------------------1.00--------------------1.00---1.00---------1.00--------------------------------1.00---------------1.00---1.00-----
................................................................................................................................................................................TGTCCTCTTTCTTCCTGGCTCT....................................................2216.006.00-1.00-----1.00----1.00-1.00---------------------------------------------1.00----------------------------1.00----------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCCAT..........................................................................................2316.0013.003.00-2.00----------1.00--------------------------------------------------------------------------------------------------
.......................................................................................................................................................................................................................TACGAAGTGATGAGCAAACTGGAGAAA........2716.006.00------2.00----------1.00-------------------1.00-1.00---1.00--------------------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCCATT.........................................................................................2416.0013.002.00------------------1.001.00---------------1.00-------------------------------------------------------------------1.00-------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCCAGT.........................................................................................2415.002.00---4.00--------1.00---------------------------------------------------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCCAA..........................................................................................2315.0013.003.00------1.00--------------------------------------------------------------------------------1.00-----------------------
................................................................................................................................................................................TGTCCTCTTTCTTCCTGGCTCTAGT.................................................2515.005.00-1.00----------1.00----------1.00--------------------------------------1.00--------------1.00----------------------------------
.......................................................................................................................................................................................................................TACGAAGTGATGAGCAAACTGGAGA..........2514.004.00------1.00----------------------------------------------1.00--------------------------1.00------------1.00------------------
........................................................................................................................................GCTGACTGGGAAGGAAATGGCCA...........................................................................................2314.004.003.00--------------------------------------------------------------------1.00------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAA..........................................................................................2214.0014.001.00----1.00----------------2.00-----------------------------------------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAT..........................................................................................2214.0014.00----1.00--2.00----------------------1.00---------------------------------------------------------------------------------
........................................................................................................................................GCTGACTGGGAAGGAAATGGCC............................................................................................2214.004.00--1.00------1.00---1.00--------------------------1.00-----------------------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAGGT........................................................................................2414.004.00-----------1.00----------------------------1.00-------------2.00---------------------------------------------------------
...................................................................................................................................................................................................................TCTCTACGAAGTGATGAGCAAACTGG.............2614.004.00-----------------1.00-----------------1.00---------------2.00------------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGC.............................................................................................2014.004.002.001.00-------------------------------------------------------------------------1.00------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAGG.........................................................................................2314.004.00--------1.00--------------1.00-------------1.00----------------------------1.00---------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAGAA........................................................................................2413.0012.00----------------1.00-----------1.00-------------1.00---------------------------------------------------------------------
................................................................................................................................................................................TGTCCTCTTTCTTCCTGGCTCTAGATT...............................................273.000.00---3.00------------------------------------------------------------------------------------------------------------
............................................................................................................................................ACTGGGAAGGAAATGGCCAGG.........................................................................................2113.003.00-2.00----------1.00---------------------------------------------------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAGAT........................................................................................2413.0012.00----2.00----------------------------------------------------------------------------1.00------------------------------
...........................................................................................................................................GACTGGGAAGGAAATGGCCAGA.........................................................................................2213.001.00-----------------------------------------3.00----------------------------------------------------------------------
........................................................................................................................................GCTGACTGGGAAGGAAATGGC.............................................................................................2113.003.001.00-1.00------1.00------------------------------------------------------------------------------------------------------
................................................................................................................................................................................TGTCCTCTTTCTTCCTGGCTCTAT..................................................243.000.00----1.00-----------------------1.00-------1.00---------------------------------------------------------------------------
................................................................................................................................................................................TGTCCTCTTTCTTCCTGGCT......................................................2013.003.00-2.00------------1.00-------------------------------------------------------------------------------------------------
.....................................................................................................................................................................................................................TCTACGAAGTGATGAGCAAACTGGAG...........2613.003.00-----------------2.00---------------------------------------1.00------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCCAAT.........................................................................................2412.0013.00-------1.00--------1.00-----------------------------------------------------------------------------------------------
......................................................................................................................................AAGCTGACTGGGAAGGAAATGG..............................................................................................2212.002.001.00------------------------------------------------------------------------------------------1.00--------------------
...................................................................................................................................................................................................................................AGCAAACTGGAGAAACAACA...2012.002.00---------------1.00---------1.00--------------------------------------------------------------------------------------
.................................................................................................................................................................................................................................TGAGCAAACTGGAGAAACAACA...2212.002.00--------------------------1.00----------------------1.00--------------------------------------------------------------
........................................................................................................AGGGTGGGCCAGAGGGA.................................................................................................................................172.000.00----------2.00-----------------------------------------------------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCCATTA........................................................................................2512.0013.00-------2.00--------------------------------------------------------------------------------------------------------
.........................................................................................................................................................................................................................CGAAGTGATGAGCAAACTGGA............2112.002.00----------------------------------------------2.00-----------------------------------------------------------------
........................................................................................................................................GCTGACTGGGAAGGAAATGGCCAA..........................................................................................2412.004.001.00-1.00-------------------------------------------------------------------------------------------------------------
...........................................................................................................................................GACTGGGAAGGAAATGGCCAGGT........................................................................................2312.001.00------------1.00--------------1.00------------------------------------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCAA...........................................................................................2212.004.001.00----------------------------------------------1.00----------------------------------------------------------------
.......................................................................................................................................................................................................................TACGAAGTGATGAGCAAACTGGAGAAACT......2912.007.00------------------2.00---------------------------------------------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGG..............................................................................................1822.002.001.50--------------0.50------------------------------------------------------------------------------------------------
........................................................................................................................................GCTGACTGGGAAGGAAATGG..............................................................................................2012.002.00--------------------------------------1.00-1.00-----------------------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCATTTT.......................................................................................2512.0014.00---------1.00---1.00--------------------------------------------------------------------------------------------------
.................................................................................................................................................................................................................................TGAGCAAACTGGAGAAACAAC....2112.002.00--------------------------2.00-------------------------------------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCCATA.........................................................................................2412.0013.001.00-----------------------------------1.00---------------------------------------------------------------------------
................................................................................CAATGGACCAAGGAGTCTA.......................................................................................................................................................192.000.00--------------------------------------------------------------1.00---------1.00---------------------------------------
..................................................................................................................................................................................TCCTCTTTCTTCCTGGCT......................................................1812.002.00-------------1.00---------------1.00----------------------------------------------------------------------------------
........................................................................................................................................GCTGACTGGGAAGGAAATGGCCAAA.........................................................................................2512.004.001.00-1.00-------------------------------------------------------------------------------------------------------------
.....................................................GGGAGGGGGGGGGGGGTGG..................................................................................................................................................................................192.000.00----------1.00-------------------------------------1.00---------------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCCAG..........................................................................................2312.002.00--------------------------------2.00-------------------------------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCATTT........................................................................................2412.0014.00--1.00----------------1.00--------------------------------------------------------------------------------------------
........................................................................................................................................GCTGACTGGGAAGGAAATGGCCATT.........................................................................................2512.004.00--1.00------------------------------------------------------------------------------------------------------1.00------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAAA.........................................................................................2312.0014.00-----1.00-------------------------------------------------------------1.00--------------------------------------------
...................................................................................................................................................................................................................TCTCTACGAAGTGATGAGCAAACTGGA............2712.002.00-----------------1.00--------------------------------------------------------1.00-------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCCAGAT........................................................................................2512.002.00-1.00------------------1.00-------------------------------------------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGG..............................................................................................1921.501.50----------------0.50------------------------------------------------------------------1.00----------------------------
............................................................................................................................................ACTGGGAAGGAAATGGCCACG.........................................................................................211.000.00-1.00--------------------------------------------------------------------------------------------------------------
..................................................TGAGGGAGGGGGGGGTCT......................................................................................................................................................................................181.000.00---------------------------------------------------------------------------------------1.00------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAGTAT.......................................................................................2511.0012.00--------1.00-------------------------------------------------------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCCAAAT........................................................................................2511.0013.001.00---------------------------------------------------------------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGCCA............................................................................................211.000.00------------------------------------------------------------------------------------1.00---------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCCATG.........................................................................................2411.0013.00--------1.00-------------------------------------------------------------------------------------------------------
........................................................................................................................................GCTGACTGGGAAGGAAATGGCAT...........................................................................................2311.003.00---------1.00------------------------------------------------------------------------------------------------------
................................................................................................................................................................................TGTCCTCTTTCTTCCGCCC.......................................................191.000.00-------------------------------------------------------1.00--------------------------------------------------------
................................................................................................................................................................................TGTCCTCTTTCTTCCTGGCTCTAGTT................................................2611.001.00-----------1.00----------------------------------------------------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAATA........................................................................................2411.0014.00----------------------------------------------------------------------1.00-----------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCATAT........................................................................................2411.0014.00--------1.00-------------------------------------------------------------------------------------------------------
..................................................TGAGGGAGGGGGGGGTAA......................................................................................................................................................................................181.000.00------------------------1.00---------------------------------------------------------------------------------------
...........................................................................................................................................GACTGGGAAGGAAATGGCCAG..........................................................................................2111.001.00---------------------------1.00------------------------------------------------------------------------------------
...................................................GAGGGAGGGGGGGGGGCGA....................................................................................................................................................................................191.000.00----------1.00-----------------------------------------------------------------------------------------------------
................................................................................................................................................................................TGTCCTCTTTCTTCCTGGCTCTAGC.................................................251.000.00-1.00--------------------------------------------------------------------------------------------------------------
...................................................................................................................................................................................................................TCTCTACGAAGTGATGAGCAAACTGGT............2711.004.00------------------1.00---------------------------------------------------------------------------------------------
..................................................................................................................................................................................TCCTCTTTCTTCCTGGCTCTAG..................................................2211.001.00-----------1.00----------------------------------------------------------------------------------------------------
..................................................TGAGGGAGGGGGGGGATGA.....................................................................................................................................................................................191.000.00-------------------------------------------------------------------------------------------------------1.00--------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCCATTT........................................................................................2511.0013.00------------------------------------------1.00---------------------------------------------------------------------
................................................................................................................................................................................................................CAATCTCTACGAAGTAAA........................181.000.00-------------------------------------------------------1.00--------------------------------------------------------
.....................................................................................................................................................................................................................................CAAACTGGAGAAACAACA...1811.001.00---------------1.00------------------------------------------------------------------------------------------------
.........................................................................................................................................................................................................................CGAAGTGATGAGCAAACTGGAGAAACAAC....2911.001.00-------------------------------------1.00--------------------------------------------------------------------------
...........................................................................................................................................................................................................................AAGTGATGAGCAAACTGGAG...........2011.001.00-------------------------------------------------------------------------------------------------1.00--------------
......................................................................................................................................AAGCTGACTGGGAAGGAAATGGC.............................................................................................2311.001.00-------------------------------------------------------------------------1.00--------------------------------------
..................................................TGAGGGAGGGGGGGGTATA.....................................................................................................................................................................................191.000.00------------------------------------------------1.00---------------------------------------------------------------
................................................................................................................................................................................................................................ATGAGCAAACTGGAGAAACA......2011.001.00-------1.00--------------------------------------------------------------------------------------------------------
..................................................................................................................................................................................TCCTCTTTCTTCCTGGCTCT....................................................2011.001.00----1.00-----------------------------------------------------------------------------------------------------------
...........................................................................................................................................GACTGGGAAGGAAATGGCCAT..........................................................................................211.000.00-1.00--------------------------------------------------------------------------------------------------------------
....................................................AGGGAGGGGGGGGGGTGGG...................................................................................................................................................................................191.000.00------------------------------------------------------------------------------------------------------------1.00---
....................................................................................................................................................................................................................................GCAAACTGGAGAAACAACA...1911.001.00-----------------------------------------------1.00----------------------------------------------------------------
..................................................TGAGGGAGGGGGGGGTAT......................................................................................................................................................................................181.000.00----------1.00-----------------------------------------------------------------------------------------------------
..................................................TGAGGGAGGGGGGGGGTGT.....................................................................................................................................................................................191.000.00-------------------------------1.00--------------------------------------------------------------------------------
..............................................................................................................................................................................................................................TGATGAGCAAACTGGAG...........1711.001.00--------------------------------------------1.00-------------------------------------------------------------------
................................................................................................................................................................................TGTCCTCTTTCTTCCTGGCTC.....................................................2111.001.00-----1.00----------------------------------------------------------------------------------------------------------
............................................................................................................................................................................................................................AGTGATGAGCAAACTGGAGAAACAACAT..2811.001.00---------------------------------------------1.00------------------------------------------------------------------
....................................................................................................GAGCAGGGTGGGCCAGGATT..................................................................................................................................201.000.00------------------------1.00---------------------------------------------------------------------------------------
..............................................................GGGGGGGGAGGAAGGTGG..........................................................................................................................................................................181.000.00------------------------------------------------------------------------------------------------1.00---------------
.....................................................GGGAGGGGGGGGGGGGTGT..................................................................................................................................................................................191.000.00----------1.00-----------------------------------------------------------------------------------------------------
...............................................................................................................................................GGGAAGGAAATGGCCAGGGCT......................................................................................2111.001.00--------------------------------------------------------1.00-------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAAAAA.......................................................................................2511.0014.00------------------------------1.00---------------------------------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCCATAT........................................................................................2511.0013.00---------------------------------1.00------------------------------------------------------------------------------
......................................................................................................................................................................................CTTTCTTCCTGGCTCTCG..................................................181.000.00----------------------------------------------------------------1.00-----------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCATAAA.......................................................................................2511.0014.00---------------------------------1.00------------------------------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCCAGGT........................................................................................251.000.00-------------------------------------------------------------1.00--------------------------------------------------
..................................................TGAGGGAGGGGGGGGCTTG.....................................................................................................................................................................................191.000.00--------------------------------------------------1.00-------------------------------------------------------------
.................................................TTGAGGGAGGGGGGGGGGGGGACA.................................................................................................................................................................................241.000.00------------------------------------------------------------------------------------------1.00---------------------
........................................................................................................................................GCTGACTGGGAAGGAAATGGCCAT..........................................................................................2411.004.00--1.00-------------------------------------------------------------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAATTT.......................................................................................2511.0014.00---------1.00------------------------------------------------------------------------------------------------------
......................................................GGAGGGGGGGGGGGGGAAAA................................................................................................................................................................................201.000.00-----------------------------------------------------------------1.00----------------------------------------------
.......................................................................................................................................................................................................................TACGAAGTGATGAGCAAACTGGAGAAACA......2911.001.00------------------1.00---------------------------------------------------------------------------------------------
.........................................................................................................................................................................................................TGAACCACAATCTCTACGAAGTGATGAGC....................2911.001.00----------------------------------------------------1.00-----------------------------------------------------------
.....................................................................GAGGAAGGGAACAATGGACC.................................................................................................................................................................2011.001.00-----------1.00----------------------------------------------------------------------------------------------------
................................................................................................................................................................................................................................ATGAGCAAACTGGAGAAAC.......1911.001.00-------------------------1.00--------------------------------------------------------------------------------------
...................................................................................................................................................................................................................................AGCAAACTGGAGAAACAACT...2011.001.00--------------------------1.00-------------------------------------------------------------------------------------
..................................................TGAGGGAGGGGGGGGTAG......................................................................................................................................................................................181.000.00------------------------1.00---------------------------------------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGAA............................................................................................2121.001.50--------0.50------------------------------------------------------------------------------------------------------0.50
..........................................................................................................................................TGACTGGGAAGGAAATGGCCACT.........................................................................................2311.0014.00--------------------1.00-------------------------------------------------------------------------------------------
...................................................................................................................................................................................................................................AGCAAACTGGAGAAACAAC....1911.001.00------------------------------1.00---------------------------------------------------------------------------------
............................................................................................................................................ACTGGGAAGGAAATGGCCAGT.........................................................................................211.000.00-1.00--------------------------------------------------------------------------------------------------------------
...........................................................................................................................................GACTGGGAAGGAAATGGCCAGGA........................................................................................2311.001.00--1.00-------------------------------------------------------------------------------------------------------------
.................................................................................................................................................................................................................................TGAGCAAACTGGAGAAACA......1911.001.00-------------------------1.00--------------------------------------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAAAT........................................................................................2411.0014.001.00---------------------------------------------------------------------------------------------------------------
............................................................................................................................................ACTGGGAAGGAAATGGCCAGA.........................................................................................211.000.00------------1.00---------------------------------------------------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAAAA........................................................................................2411.0014.00--------------1.00-------------------------------------------------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCCATAAA.......................................................................................2611.0013.00-------------------1.00--------------------------------------------------------------------------------------------
...................................................................................................................................................................................................................TCTCTACGAAGTGATGAGCAAACTGGAT...........2811.002.00-----------------------------------1.00----------------------------------------------------------------------------
............................................................................................................................................ACTGGGAAGGAAATGGACC...........................................................................................191.000.00-----------1.00----------------------------------------------------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCCATATA.......................................................................................2611.0013.00--1.00-------------------------------------------------------------------------------------------------------------
.......................................................................................................................................................................................................................TACGAAGTGATGAGCAAACTGGAGAT.........2611.004.00------1.00---------------------------------------------------------------------------------------------------------
.......................................................................................................................................AGCTGACTGGGAAGGAAATGGCCAT..........................................................................................251.000.00--1.00-------------------------------------------------------------------------------------------------------------
..................................................TGAGGGAGGGGGGGGGATG.....................................................................................................................................................................................191.000.00--------------------------------------------------1.00-------------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCATT.........................................................................................2311.0014.00----------------------------------1.00-----------------------------------------------------------------------------
...................................................GAGGGAGGGGGGGGGGTGG....................................................................................................................................................................................191.000.00-------------------------------1.00--------------------------------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAGGC........................................................................................2411.004.00-----------1.00----------------------------------------------------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAGGTAT......................................................................................2611.004.00-1.00--------------------------------------------------------------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAACGG..............................................................................................191.000.00---------1.00------------------------------------------------------------------------------------------------------
.................................................................................................................................................................................................................................TGAGCAAACTGGAGAAACAACAGT.2411.002.00----------------------------------------------------------1.00-----------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCCAAAGA.......................................................................................2611.0013.00--------1.00-------------------------------------------------------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAGGA........................................................................................2411.004.00-----------------------------1.00----------------------------------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGTCCA...........................................................................................221.000.00-----------------------------1.00----------------------------------------------------------------------------------
............................................................................................................................................ACTGGGAAGGAAATGGCCAGGTTTT.....................................................................................2511.003.00-1.00--------------------------------------------------------------------------------------------------------------
.............................................................................................................................TGATTGGGAAAGCTGACTGGGAAG.....................................................................................................2411.001.00----1.00-----------------------------------------------------------------------------------------------------------
..................................................TGAGGGAGGGGGGGGTGTA.....................................................................................................................................................................................191.000.00------------------------1.00---------------------------------------------------------------------------------------
...........................................................................................................................................GACTGGGAAGGAAATGGCC............................................................................................1911.001.00---------------------------1.00------------------------------------------------------------------------------------
.......................................................................................................................................................AATGGCCAGGGCTCTCTAACTAACC..........................................................................2511.001.00------------------------------------------------------------------------------1.00---------------------------------
.....................................................................................................................................................................................................................TCTACGAAGTGATGAGCAAACTGGAGA..........2711.001.00---------------------------------------------1.00------------------------------------------------------------------
..................................................TGAGGGAGGGGGGGGGAGCT....................................................................................................................................................................................201.000.00----------1.00-----------------------------------------------------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCCATTTT.......................................................................................2611.0013.001.00---------------------------------------------------------------------------------------------------------------
.........................................................................................................................................CTGACTGGGAAGGAAATGGCC............................................................................................2111.001.00--1.00-------------------------------------------------------------------------------------------------------------
.......................................................................................................................................................................................................................TACGAAGTGATGAGCAAACTGGAG...........2411.001.00--------------------------------------------1.00-------------------------------------------------------------------
..................................................TGAGGGAGGGGGGGGAAGC.....................................................................................................................................................................................191.000.00----------1.00-----------------------------------------------------------------------------------------------------
........................................................................................................................................................................................................................ACGAAGTGATGAGCAAACT...............1911.001.00-----------------------------------------------------------------------1.00----------------------------------------
..................................................................................................................................................................................TCCTCTTTCTTCCTGGCTTT....................................................2011.002.00-1.00--------------------------------------------------------------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGCCAGTTT.......................................................................................2511.0012.00-----------------------1.00----------------------------------------------------------------------------------------
...........................................................................................................................................GACTGGGAAGGAAATGGCCAGG.........................................................................................2211.001.00------------1.00---------------------------------------------------------------------------------------------------
..............................................................................................................................................................................................................................TGATGAGCAAACTGGAGAAACAACATTC2811.001.00----------------------------------------------------1.00-----------------------------------------------------------
..................................................TGAGGGAGGGGGGGGTAGG.....................................................................................................................................................................................191.000.00-------------------------------1.00--------------------------------------------------------------------------------
...........................................................................................................................................................................................................................AAGTGATGAGCAAACTGT.............181.000.00----------------------------------------------------------------------------------------------------1.00-----------
......................................................................................................................................................................................CTTTCTTCCTGGCTCTAG..................................................1820.500.50----------------------------------------------------------------0.50-----------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATGGAC............................................................................................2020.502.00-----0.50----------------------------------------------------------------------------------------------------------
..........................................................................................................................................TGACTGGGAAGGAAATG...............................................................................................1760.170.17-----------------------------------------------------------------0.17----------------------------------------------
...............................................................................ACAATGGACCAAGGA............................................................................................................................................................1570.140.14--------------------------------------------0.14-------------------------------------------------------------------

Antisense strand
CCTGAGGGAAACCCTACCCTGAGAGAGACCCTGCCCTGAGTGAGCTACCTTGAGGGAGGGGGGGGGGGGGAGGAAGGGAACAATGGACCAAGGAGTAAAAGAGCAGGGTGGGCCAGAGGAGGTTTTGATTGGGAAAGCTGACTGGGAAGGAAATGGCCAGGGCTCTCTAACTAACCTGTCCTCTTTCTTCCTGGCTCTAGTTGAACCACAATCTCTACGAAGTGATGAGCAAACTGGAGAAACAACATTC
...........................................................................................................................................(((((((((((((..((.((((.............)))).))..))))))))))).)).....................................................
.....................................................................................................................................134...............................................................200................................................
SizePerfect hitTotal NormPerfect NormSRR042471(GSM539863)
mouse TFHS cells [09-002]. (spleen)		SRR059769(GSM562831)
Treg_Drosha. (spleen)		SRR042458(GSM539850)
mouse basophil cells [09-002]. (b cell)		mjLiverKO1()
Liver Data. (Zcchc11 liver)		SRR042459(GSM539851)
mouse neutrophil cells replicate 1 [09-002]. (blood)		SRR042470(GSM539862)
mouse Th17 cells [09-002]. (lymph)		SRR028732(GSM400969)
Mili-Tdrd1 KO associated. (mili testes)		SRR042456(GSM539848)
mouse hematopoetic progenitor cells [09-002]. (bone marrow)		SRR042469(GSM539861)
mouse Th2 cells [09-002]. (lymph)		SRR042472(GSM539864)
mouse iTreg cells [09-002]. (lymph)		SRR345200(SRX097261)
source: size fractionated RNA from mouse hipp. (brain)		GSM519104(GSM519104)
mouse_granulocyte_nuclei_smallRNAs. (cell line)		SRR059770(GSM562832)
Treg_Dicer. (spleen)		SRR042465(GSM539857)
mouse CD4+ T cells (spleen) [09-002]. (spleen)		SRR042466(GSM539858)
mouse CD8+ T cells (spleen) [09-002]. (spleen)		SRR042450(GSM539842)
mouse B cells activated in vitro with LPS/IL4 replicate 3 [09-002]. (b cell)		SRR042484(GSM539876)
mouse salivary gland tissue [09-002]. (gland)		SRR034118(GSM466728)
Mili IP_Tdrd9+/- replicate1. (mili testes)		GSM475280(GSM475280)
Mili-IP. (mili testes)		SRR042447(GSM539839)
mouse mature B cells (spleen) replicate 2 [09-002]. (b cell)		SRR042488(GSM539880)
mouse BclXL germinal center B cells [BalbC] [09-002]. (B cell)		mjLiverWT1()
Liver Data. (liver)		SRR042463(GSM539855)
mouse natural killer cells [09-002]. (spleen)		SRR059766(GSM562828)
DN3_Drosha. (thymus)		SRR345196(SRX097257)
source: size fractionated RNA from mouse hipp. (brain)		SRR042449(GSM539841)
mouse B cells activated in vitro with LPS/IL4 replicate 2 [09-002]. (b cell)		SRR042448(GSM539840)
mouse B cells activated in vitro with LPS/IL4 replicate 1 [09-002]. (b cell)		SRR059772(GSM562834)
CD4_Drosha. (spleen)		SRR525244(SRA056111/SRX170320)
Mus musculus domesticus miRNA sequencing. (ago2 Blood)		SRR042455(GSM539847)
mouse plasma cells [09-002]. (blood)		SRR042487(GSM539879)
mouse BclXL B activated with LPS/IL4 [BalbC] [09-002]. (spleen)		SRR345198(SRX097259)
source: size fractionated RNA from mouse hipp. (brain)		SRR346414(SRX098255)
Global profiling of miRNA and the hairpin pre. (Spleen)		SRR042473(GSM539865)
mouse nTreg cells [09-002]. (lymph)		SRR042483(GSM539875)
mouse muscle tissue [09-002]. (muscle)		SRR034120(GSM466730)
Mili IP_Tdrd9-/- replicate1. (mili testes)		SRR042462(GSM539854)
mouse macrophages [09-002]. (bone marrow)		SRR248523(GSM733811)
cell type: Thy1+ spermatogonial stem cellstra. (testes)		SRR042457(GSM539849)
mouse mast cells [09-002]. (bone marrow)		GSM509275(GSM509275)
MitoPLD+/+ E16.5 small RNA. (testes)		SRR525242(SRA056111/SRX170318)
Mus musculus domesticus miRNA sequencing. (ago2 Blood)		SRR059771(GSM562833)
CD4_control. (spleen)		SRR042461(GSM539853)
mouse dendritic cells [09-002]. (bone marrow)		GSM509279(GSM509279)
MVH-/- E16.5 small RNA. (testes)		SRR248524(GSM733812)
cell type: Thy1+ spermatogonial stem cellstra. (testes)		SRR029037(GSM433289)
18dpp_homo_tdrd6-KO. (tdrd6 testes)		mjTestesKO7()
Testes Data. (Zcchc11 testes)		SRR042467(GSM539859)
mouse T cells activated in vitro with Concanavalin A [09-002]. (spleen)		SRR345199(SRX097260)
source: size fractionated RNA from mouse hipp. (brain)		SRR042468(GSM539860)
mouse Th1 cells [09-002]. (lymph)		SRR345203(SRX097264)
source: size fractionated RNA from mouse hipp. (brain)		mjTestesWT3()
Testes Data. (testes)		SRR029038(GSM433290)
25dpp_hetero_tdrd6-KO. (tdrd6 testes)		SRR037901(GSM510437)
testes_rep2. (testes)		SRR525238(SRA056111/SRX170314)
Second IgG control. (Blood)		SRR073954(GSM629280)
total RNA. (blood)		GSM509277(GSM509277)
small RNA cloning by length. (piwi testes)		SRR034119(GSM466729)
Mili IP_Tdrd9+/- replicate2. (mili testes)		SRR028730(GSM400967)
Tdrd1-associated. (tdrd1 testes)		mjLiverKO3()
Liver Data. (Zcchc11 liver)		SRR059765(GSM562827)
DN3_control. (thymus)		SRR346419(SRX098260)
Global profiling of miRNA and the hairpin pre. (Lung)		GSM314557(GSM314557)
ESC dgcr8 (Illumina). (ESC)		SRR042460(GSM539852)
mouse neutrophil cells replicate 2 [09-002]. (blood)		SRR037929(GSM510467)
e7p5_rep3. (embryo)		SRR346425(SRX098267)
Global profiling of miRNA and the hairpin pre. (Neuroblastoma)		SRR391845(GSM852140)
gender: femalegenotype/variation: wild typeti. (embryo)		SRR051940(GSM545784)
18-32 nt total small RNAs (Mov10l+/-). (testes)		GSM510444(GSM510444)
brain_rep5. (brain)		SRR042454(GSM539846)
mouse germinal center B cells (lymph node) [09-002]. (b cell)		SRR065054(SRR065054)
Tissue-specific Regulation of Mouse MicroRNA . (jejunum)		SRR391846(GSM852141)
gender: femalegenotype/variation: ADAR2-/-tis. (embryo)		GSM314553(GSM314553)
ESC dcr (Illumina). (ESC)		SRR042452(GSM539844)
mouse B1 B cells [09-002]. (b cell)		SRR034121(GSM466731)
Mili IP_Tdrd9-/- replicate2. (mili testes)		SRR553602(SRX182808)
source: Sertoli cells. (Sertoli cells)		GSM509278(GSM509278)
small RNA cloning by length. (piwi testes)		SRR038743(GSM527278)
small RNA-Seq. (dicer brain)		SRR391853(GSM852148)
gender: femalegenotype/variation: ADAR2-/-/AD. (embryo)		SRR402762(SRX117945)
source: ES E14 male cells. (ESC)		SRR028731(GSM400968)
Mili-wt-associated. (testes)		SRR525237(SRA056111/SRX170313)
Mus musculus domesticus miRNA sequencing. (Blood)		SRR042480(GSM539872)
mouse kidney tissue [09-002]. (kidney)		SRR023852(GSM307161)
ZHBT-c424hsmallrna_rep1. (cell line)		SRR391851(GSM852146)
gender: femalegenotype/variation: wild typeti. (embryo)		GSM640578(GSM640578)
small RNA in the liver with paternal control. (liver)		SRR248527(GSM733815)
cell type: spermatogonial stem cell enriched . (testes)		SRR345207(SRX097268)
source: size fractionated RNA from mouse hipp. (brain)		SRR042464(GSM539856)
mouse CD4+CD8+ T cells (thymus) [09-002]. (thymus)		SRR525240(SRA056111/SRX170316)
Mus musculus domesticus miRNA sequencing. (ago1 Blood)		SRR345205(SRX097266)
source: size fractionated RNA from mouse hipp. (brain)		SRR065055(SRR065055)
Tissue-specific Regulation of Mouse MicroRNA . (jejunum)		SRR042443(GSM539835)
mouse pro B cells [09-002]. (b cell)		SRR069811(GSM610967)
small RNA sequencing; sample 3. (testes)		SRR391852(GSM852147)
gender: femalegenotype/variation: ADAR2-/-tis. (embryo)		SRR306536(GSM750579)
19-24nt. (ago2 brain)		SRR279904(GSM689054)
cell type: mouse embryonic stem cellcell line. (ESC)		SRR040488(GSM533911)
E18.5 wild type LIVER small RNA. (liver)		SRR059768(GSM562830)
Treg_control. (spleen)		SRR051939(GSM545783)
Mov10L1-associated piRNAs. (mov10L testes)		SRR059773(GSM562835)
CD4_Dicer. (spleen)		mjLiverWT3()
Liver Data. (liver)		SRR014231(GSM319955)
16.5 dpc total. (testes)		SRR345201(SRX097262)
source: size fractionated RNA from mouse hipp. (brain)		SRR346415(SRX098256)
source: Testis. (Testes)		SRR042445(GSM539837)
mouse immature B cells [09-002]. (b cell)		SRR363956(GSM822758)
P14-WTSmall RNA Miwi IPread_length: 36. (testes)		SRR037902(GSM510438)
testes_rep3. (testes)		SRR077866(GSM637803)
18-30 nt small RNAs. (liver)		SRR037924(GSM510462)
e9p5_rep2. (embryo)		SRR042474(GSM539866)
mouse embryonic stem cells [09-002]. (ESC)		SRR042446(GSM539838)
mouse mature B cells (spleen) replicate 1 [09-002]. (b cell)
..............................................................................................................................................................AGGGCTCTCTAACTAACCTGTCC..................................................................... 2311.001.00-------------------------------------------------------------------------------1.00--------------------------------
................................GCCCTGAGTGAGCTAGGT........................................................................................................................................................................................................ 181.000.00---------------------------1.00------------------------------------------------------------------------------------
..........................GACCCTGCCCTGAGTGTTG............................................................................................................................................................................................................. 191.000.00-----------------------------------------------------------------------------------------------1.00----------------