ID: uc010icg.1_intron_10_0_chr4_2158581_r.5p
GENE: POLN(2)
chr4:2159385-2159634-


(1)
AGO3.ip 		(1)
BRAIN 		(15)
CELL-LINE 		(1)
CERVIX 		(2)
HELA 		(1)
LIVER 		(2)
OTHER 		(5)
SKIN

Sense strand
ACAAGATCAAGTCAACCTTTGTAGATGGATTACTAGCTTGCATGAAAAAGGTAACAAGCTTTACAAAAAGATACTGTGATTAGGACTGTGGTTTTTGTATGAGTTCAGATACATATAGAACCTTTGTAATTTTAGTAAATGATGAAATCGTCTAAATATTCTTTTTTTTTTTTTTTTTTTTTTTTGAGACAGAGTCTTGCACTGTCGCCCGGGCTGGAGTGCAGTGTCAGGATCTCAGCTCACTGCAACC
.......................................................................(((((.(((((.((....((.(((((((((.((....)).))))))))))).)).))))).)))))...((((....))))..................................................................................................
....................................................................69..................................................................................................................185...............................................................
SizePerfect hitTotal NormPerfect NormSRR189780(GSM714640)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR189776(GSM714636)
cell line: HEK293clip variant: CLIPenzymatic . (cell line)		SRR189775(GSM714635)
cell line: HEK293clip variant: CLIPenzymatic . (cell line)		SRR189777(GSM714637)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR189779(GSM714639)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR189778(GSM714638)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR189781(GSM714641)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR095854(SRX039177)
"miRNA were isolated from FirstChoice Human B. (brain)		SRR037943(GSM510481)
293DcrTN. (cell line)		SRR189786SRR553573(SRX182779)
source: Cerebellum. (Cerebellum)		SRR039614(GSM531977)
HBV-infected Liver Tissue. (liver)		SRR189787GSM532886(GSM532886)
G850T. (cervix)		DRR001488(DRX001042)
"Hela short nuclear cell fraction, LNA(+)". (hela)		SRR330908(SRX091746)
tissue: normal skindisease state: normal. (skin)		DRR000555(DRX000313)
"THP-1 whole cell RNA, no treatment". (cell line)		DRR000562(DRX000320)
Isolation of RNA following immunoprecipitatio. (ago3 cell line)		SRR330888(SRX091726)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR039190(GSM494809)
PBMCs were isolated by ficoll gradient from t. (blood)		SRR189783GSM1105753INPUT(GSM1105753)
small RNA sequencing data. (hela)		DRR000558(DRX000316)
"THP-1 whole cell RNA, after 3 day treatment . (cell line)		SRR390725(GSM850204)
small rna immunoprecipitated. (cell line)		SRR330868(SRX091706)
tissue: skin psoriatic involveddisease state:. (skin)		SRR330877(SRX091715)
tissue: skin psoriatic involveddisease state:. (skin)		SRR330902(SRX091740)
tissue: skin psoriatic uninvolveddisease stat. (skin)		GSM956925Paz8D5(GSM956925)
"cell line: HEK293 cell linepassages: 15-20ip. (cell line)		SRR060984(GSM569188)
Human plasma cell [09-001]. (cell line)		SRR038856(GSM458539)
D11. (cell line)
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTGGTT.............................................................3281.000.0070.00---10.00----1.00--------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTGGTT...................................................................2732.000.0027.00---5.00-------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTATG..............................................................3118.000.00-8.006.00--4.00------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTCG..................................................................298.000.001.001.002.004.00--------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTCGG..................................................................287.000.004.00---2.00-1.00-----------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTCAAG..............................................................317.000.001.00-1.003.00--2.00-----------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTCG.................................................................306.000.00-2.00-3.00--1.00-----------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTCTGG..................................................................286.000.006.00-----------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTCCAG..............................................................315.000.00-1.001.001.00-1.001.00-----------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTCGG................................................................305.000.004.00---1.00-------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTCG....................................................................265.000.00--2.001.001.001.00------------------------
................................................................................................................................................................................................................CCGGGCTGGAGTGCAGTGTAGGG...................234.000.00-------4.00----------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTTATG..............................................................324.000.00-3.001.00---------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTTAAA..............................................................324.000.00-1.002.00--1.00------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTC.................................................................304.000.001.001.001.001.00--------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTATG................................................................314.000.00-1.002.001.00--------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTCAAG.................................................................294.000.00-1.001.00--1.001.00-----------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTGGTTT............................................................334.000.004.00-----------------------------
.....................TAGATGGATTACTAGCTTGCATGAAAAAG........................................................................................................................................................................................................2914.004.00--------4.00---------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTCGG...................................................................274.000.003.00---1.00-------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTAGG..............................................................314.000.00-2.002.00---------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTCG....................................................................274.000.00---1.00-3.00------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTCTG...............................................................323.000.00--2.001.00--------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTTGTT..............................................................323.000.003.00-----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTCTGG.................................................................293.000.002.00---1.00-------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTCTTG..............................................................323.000.00-1.001.00-1.00-------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTGGGTT............................................................333.000.003.00-----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTGTTTT............................................................353.000.00-1.002.00---------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTATTG...............................................................313.000.00-2.00-1.00--------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTCTG..............................................................333.000.00-1.001.00--1.00------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTCTG..................................................................293.000.00-2.001.00---------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTCG...................................................................283.000.00--1.001.00-1.00------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTTGTTTT............................................................343.000.00--2.00--1.00------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTTGGT..............................................................323.000.003.00-----------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTCTAG..............................................................313.000.00-1.00-1.001.00-------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTAAAA...............................................................313.000.00-2.00-1.00--------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTCTT..............................................................333.000.00-1.00-2.00--------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTAAAT..............................................................323.000.00--3.00---------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTAATG..............................................................322.000.00--2.00---------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTACG..............................................................312.000.00--1.001.00--------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTATCT.............................................................322.000.00-1.001.00---------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTCCTA...............................................................312.000.00--2.00---------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTCAAG.............................................................342.000.00--1.00--1.00------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTCGGG................................................................302.000.002.00-----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTAATG...............................................................312.000.00-2.00----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTATAG..............................................................322.000.00--1.001.00--------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTATTT..............................................................322.000.001.001.00----------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTCCGG..............................................................312.000.002.00-----------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTGGGT.............................................................322.000.002.00-----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTCGG.....................................................................252.000.001.00---1.00-------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTATAG..............................................................332.000.00-1.001.00---------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTCGAG............................................................................192.000.00----2.00-------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTCCGG.................................................................292.000.001.00-----1.00-----------------------
..............................................................................................................................................................................................................GCCCGGGCTGGAGTGCTCTG........................202.000.00---------------1.00----------1.00---
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTCTGG................................................................302.000.002.00-----------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTCAGG..............................................................312.000.001.00---1.00-------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTATAT.............................................................322.000.00-----1.001.00-----------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTATTG..............................................................332.000.00-1.001.00---------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTTGTTG.............................................................332.000.00-1.001.00---------------------------
.............................................................................................................................................................................................................CGCCCGGGCTGGAGTGCTCTG........................212.000.00------------------------1.001.00----
..........................................................................................................................................................................................................TGTCGCCCGGGCTGGAGTGCAGCGCG......................2602.001.002.00-----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTATG...............................................................312.000.00-1.001.00---------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTCAG..............................................................332.000.00-1.001.00---------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTCATG...............................................................322.000.00-1.001.00---------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTGT......................................................................252.000.00--2.00---------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTCGG..................................................................292.000.00-1.001.00---------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTCAAG...............................................................312.000.00-----2.00------------------------
......................................................................................................................................................TCTAAATATTCTTTTTTTTTTTTG............................................................................242.000.00--1.001.00--------------------------
............................................................................................................................................................................................................TCGCCCGGGCTGGAGTGCAGTGTCGCG...................272.000.002.00-----------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTATAG.............................................................322.000.00-1.00-1.00--------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTCCC...............................................................302.000.001.00-1.00---------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTATCG.............................................................322.000.00-2.00----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTCAAG..............................................................332.000.00-1.001.00---------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTCCTT..............................................................322.000.001.00-1.00---------------------------
.......................................................................................................................................TAAATGATGAAATCGCC..................................................................................................172.000.00-------------2.00----------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTCCAG...............................................................322.000.00-1.00---1.00------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTATTG..............................................................322.000.00-1.00----1.00-----------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTCCTG................................................................302.000.00-1.001.00---------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTTAATT.............................................................332.000.00-1.001.00---------------------------
....................................................................................................................................................................................................................................AGGATCTCAGCTCACTGCAACGAA242.000.00-----------2.00------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTCTTG..............................................................332.000.00---2.00--------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTCCG...............................................................312.000.00--1.00--1.00------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTACG................................................................301.000.00--1.00---------------------------
..............................................................................................................................................................TTCTTTTTTTTTTTTTTTTTTTTTTTTGAAAAA...........................................................331.000.001.00-----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTCGA...................................................................271.000.001.00-----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTCAAG..................................................................281.000.00---1.00--------------------------
............................................................................................................................................................................................................TCGCCCGGGCTGGAGTGCAGTCGCC.....................251.000.00----1.00-------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTAAGG.............................................................341.000.00--1.00---------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTGGCTT............................................................331.000.001.00-----------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTGAGGGTG..........................................................351.000.00-----1.00------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTCGGC................................................................301.000.001.00-----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTTCTAG.............................................................331.000.00---1.00--------------------------
..........................................................................................................................................................AATATTCTTTTTTTTTTTTTTAGCG.......................................................................251.000.00----1.00-------------------------
......................................................................................................................................................TCTAAATATTCTTTTTTTTTTTTTTTG.........................................................................271.000.00--1.00---------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTCAT.....................................................................261.000.00--1.00---------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTATTG.............................................................341.000.00-1.00----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTTAACG.............................................................331.000.00-1.00----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTGGGTT............................................................351.000.00-1.00----------------------------
..........................................................................................................................................................AATATTCTTTTTTTTTTTTTTTTTTCTAG...................................................................291.000.00------1.00-----------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTCTA..................................................................281.000.001.00-----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTATAG...............................................................321.000.00-1.00----------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTCTA...............................................................301.000.00-----1.00------------------------
...........................................................................................................................................................................................................GTCGCCCGGGCTGGAGTG.............................1801.001.00----------------------1.00-------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTGTT................................................................311.000.00--1.00---------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTAAAT...............................................................321.000.00-1.00----------------------------
..........................................................................................................................................................................................................TGTCGCCCGGGCTGGAGTGCAGCTG.......................2501.001.001.00-----------------------------
..........................................................................................................................................................................................................TGTCGCCCGGGCTGGAGTGCAGTGCGCG....................281.000.001.00-----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTCCTA..................................................................281.000.00---1.00--------------------------
..........................................................................................................................................................AATATTCTTTTTTTTTTTTTTTTTTTCTG...................................................................291.000.00-----1.00------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTATTT.............................................................321.000.00---1.00--------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTATA................................................................301.000.00-1.00----------------------------
.......................................................................................................................................................CTAAATATTCTTTTTTTTTTTTTTTTTTTTTCTG.................................................................341.000.00---1.00--------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTCCAG..................................................................281.000.00---1.00--------------------------
.........................................................................CTGTGATTAGGACTGTGGTTTTCTG........................................................................................................................................................251.000.00----------1.00-------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTCGAG..................................................................281.000.001.00-----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTCTAG..................................................................291.000.00---1.00--------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTCTTT....................................................................271.000.00-----1.00------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTGAGC................................................................311.000.00----1.00-------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTCTCC..............................................................331.000.001.00-----------------------------
.........................................................................................................................................................AAATATTCTTTTTTTTTTTTTTTTTTTTTTTAATG..............................................................351.000.00-1.00----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTAT................................................................311.000.00-1.00----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTCTA...............................................................311.000.00-1.00----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTAC...............................................................321.000.00--1.00---------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTGGGT.............................................................341.000.00--1.00---------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTCTAG.................................................................301.000.00---1.00--------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTCTA..................................................................291.000.00---1.00--------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTCCG...................................................................281.000.001.00-----------------------------
..........................................................................................................................................................AATATTCTTTTTTTTTTTTTTTTTTCCGG...................................................................291.000.001.00-----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTAGG................................................................301.000.00--1.00---------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTCAG........................................................................231.000.00---1.00--------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTGTGTT............................................................351.000.00---1.00--------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTCG................................................................311.000.00-1.00----------------------------
..........................................................................................................................................................................................................TGTCGCCCGGGCTGGAGTGCATAT........................241.000.00----------1.00-------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTCCTC..............................................................311.000.001.00-----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTCTAG.................................................................291.000.00---1.00--------------------------
........................................................................................................................................................TAAATATTCTTTTTTTTAAAC.............................................................................211.000.00--------------------1.00---------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTATAA...............................................................311.000.00-----1.00------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTCTTT.............................................................341.000.00---1.00--------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTGGCGG............................................................351.000.00-1.00----------------------------
......................................................................................................................................................TCTAAATATTCTTTTTTTTTTTTTTTTTTTTTTTG.................................................................351.000.00--1.00---------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTATCA.............................................................321.000.00-1.00----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTAAT..............................................................331.000.00--1.00---------------------------
...............................................................................................................................................................TCTTTTTTTTTTTTTTTTTTTTTTTTGAGCGGG..........................................................331.000.00----1.00-------------------------
..........................................................................................................................................................................................AGACAGAGTCTTGCACTGTCGCCCGGGCCG..................................301.000.00------1.00-----------------------
............................................................................................................................................................................................................TCGCCCGGGCTGGAGTGCAGCGCG......................241.000.001.00-----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTCGT...............................................................311.000.00---1.00--------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTCCG..................................................................291.000.00-1.00----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTTCTTG.............................................................331.000.00-1.00----------------------------
..........................................................................................................................................................AATATTCTTTTTTTTTTTTTTTTTTTTTTTTGTGG.............................................................351.000.00---1.00--------------------------
.........................................................................................................................................................AAATATTCTTTTTTTTTTTTTTTCTTG......................................................................271.000.00-----1.00------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTCTC...................................................................281.000.001.00-----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTAAAT.............................................................341.000.00-1.00----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTCTAT....................................................................271.000.00-1.00----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTCACT....................................................................261.000.001.00-----------------------------
..........................................................................................................................................................................................................TGTCGCCCGGGCTGGAGTGCAGTGTGCG....................281.000.001.00-----------------------------
..........................................................................................................................................................AATATTCTTTTTTTTTTTTTTTTTCATG....................................................................281.000.00-1.00----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTATGG..............................................................321.000.00------1.00-----------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTGGGC...................................................................271.000.001.00-----------------------------
..........................................................................................................................................................AATATTCTTTTTTTTTTTTTTTTTTTTTCTTT................................................................321.000.00-----1.00------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTCGAG..................................................................291.000.00----1.00-------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTATT................................................................311.000.00---1.00--------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTTCGG..............................................................321.000.001.00-----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTCCAT..............................................................331.000.00-1.00----------------------------
...........................................................................................................................................................................................................GTCGCCCGGGCTGGAGTGCAGCGGT......................251.000.001.00-----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTCAAG.................................................................301.000.00------1.00-----------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTAGTT.............................................................321.000.00----1.00-------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTCTCG.................................................................291.000.00---1.00--------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTATAT...............................................................311.000.00------1.00-----------------------
..........................................................................................................................................................AATATTCTTTTTTTTTTTTTTTTTTAG.....................................................................271.000.00--1.00---------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTGGTC.............................................................321.000.001.00-----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTCGTT....................................................................261.000.001.00-----------------------------
............................................................................................................................................................................................................TCGCCCGGGCTGGAGTGCAGCGAC......................241.000.001.00-----------------------------
................................................................................................................................................................................................AGTCTTGCACTGTCGCCCGGGCTGGAGCGC............................301.000.00----1.00-------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTCGAT..............................................................311.000.00----1.00-------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTCCG................................................................301.000.001.00-----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTCAG..................................................................291.000.00-1.00----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTCCAG.................................................................301.000.00-----1.00------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTTCATA.............................................................331.000.00-1.00----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTGTT..............................................................331.000.00-1.00----------------------------
.........................................................................................................................................................................................................CTGTCGCCCGGGCTGGAGTGCGGC.........................241.000.001.00-----------------------------
.........................................................................................................................................................................................................CTGTCGCCCGGGCTGGAGTGCTCTG........................251.000.00------------------1.00-----------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTCCAT...............................................................311.000.00--1.00---------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTCGAG..............................................................331.000.00-1.00----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTCCTG.............................................................341.000.00---1.00--------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTAATG..............................................................331.000.00--1.00---------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTAGG...............................................................311.000.00-1.00----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTATG...............................................................321.000.00-1.00----------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTAACA.............................................................321.000.00-1.00----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTCGGT.................................................................291.000.001.00-----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTATGT....................................................................271.000.001.00-----------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTGGCT.............................................................321.000.001.00-----------------------------
................................................................................................................................................................CTTTTTTTTTTTTTTTTTTTTTTTTGAGCGC...........................................................311.000.001.00-----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTCATC................................................................311.000.001.00-----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTATC................................................................301.000.00------1.00-----------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTTACCA.............................................................331.000.00---1.00--------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTCTTG.............................................................341.000.00--1.00---------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTCATG................................................................301.000.00-1.00----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTCTC.................................................................301.000.001.00-----------------------------
..............................................................................................................................................................TTCTTTTTTTTTTTTTTTTTTTTTTTTGAGAAAAG.........................................................351.000.00--1.00---------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTAACT.............................................................321.000.00-1.00----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTGTTG....................................................................271.000.00-----1.00------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTATAG...............................................................311.000.00-1.00----------------------------
...................................................................................................................................................................................................................GGCTGGAGTGCAGTGTCCT....................191.000.00---------------------------1.00--
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTCGA..................................................................281.000.001.00-----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTC................................................................311.000.00--1.00---------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTTATTT.............................................................331.000.00--1.00---------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTACTT.............................................................321.000.00-----1.00------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTCTTG...............................................................321.000.00--1.00---------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTAGTG.............................................................321.000.001.00-----------------------------
............................................................................................................................................................................................................TCGCCCGGGCTGGAGTGCAGTGCAC.....................251.000.001.00-----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTCGGA...............................................................311.000.00--1.00---------------------------
..........................................................................................................................................................AATATTCTTTTTTTTTTTTTTTTTCTG.....................................................................271.000.00---1.00--------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTGGTGT............................................................351.000.00--1.00---------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTATT..............................................................331.000.00-1.00----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTCCGG................................................................301.000.00-1.00----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTCCCG................................................................301.000.00--1.00---------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTCCTG.................................................................301.000.00------1.00-----------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTCTTG.................................................................301.000.00-1.00----------------------------
..........................................................................................................................................................AATATTCTTTTTTTTTTTTTTTTTTTTCAG..................................................................301.000.00---1.00--------------------------
...............................................................................................................................................................................................................CCCGGGCTGGAGTGCAGTAGT......................211.000.00-----------------1.00------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTCAAG....................................................................271.000.00-----1.00------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTAT.................................................................301.000.00-----1.00------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTCTAG..............................................................321.000.00---1.00--------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTCTCG..............................................................311.000.00-----1.00------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTCCCC..............................................................311.000.001.00-----------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTATA..............................................................311.000.00-1.00----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTCCGG..................................................................281.000.001.00-----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTCGG..............................................................331.000.00-1.00----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTCTCT.................................................................301.000.00------1.00-----------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTCCG........................................................................231.000.00-----1.00------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTAAAT...............................................................311.000.00--1.00---------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTAGG..............................................................331.000.00-1.00----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTCCAT.............................................................341.000.00-1.00----------------------------
..................................................................................................................................................................................................................GGGCTGGAGTGCAGTGTCGCGG..................221.000.00----1.00-------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTCTAG.............................................................341.000.00--1.00---------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTCATA................................................................301.000.00-1.00----------------------------
..............................................................................................................................................................................................................GCCCGGGCTGGAGTGCAGTGTAAGA...................251.000.00-------------------1.00----------
..........................................................................................................................................................................................................TGTCGCCCGGGCTGGAGTGCAG..........................2201.001.00---------------------1.00--------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTAAAG..............................................................331.000.00-1.00----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTCGAG.................................................................301.000.001.00-----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTTAATG.............................................................331.000.00---1.00--------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTGAAAG............................................................331.000.00------1.00-----------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTAGG.....................................................................261.000.00--1.00---------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTTATAA.............................................................331.000.00-1.00----------------------------
......................................................................................................................................................TCTAAATATTCTTTTTTTTTTTG.............................................................................231.000.00--1.00---------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTCAG...............................................................311.000.00-1.00----------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTCCAC..............................................................311.000.00---1.00--------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTCGT.................................................................291.000.00-1.00----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTCTTG....................................................................271.000.00---1.00--------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTCTAT...............................................................311.000.00-1.00----------------------------
....................................................................................................................................................................................................................................AGGATCTCAGCTCACTGCACCGG231.000.00--------------1.00---------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTCGGG.................................................................291.000.00-----1.00------------------------
............................................................................................................................................................................................................TCGCCCGGGCTGGAGTGC............................1801.001.00----------------1.00-------------
.....................................................................................................................................................................................................TGCACTGTCGCCCGGGCTGGAGAGCA...........................261.000.001.00-----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTCGGC.......................................................................241.000.001.00-----------------------------
..........................................................................................................................................................................................................TGTCGCCCGGGCTGGAGCGCG...........................211.000.00----1.00-------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTGAACG............................................................331.000.001.00-----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTGATG................................................................311.000.001.00-----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTACTT..............................................................331.000.00-1.00----------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTCTCC..................................................................281.000.00----1.00-------------------------
.....................................................................................................................................................................TTTTTTTTTTTTTTTTTTTTGAGACAGAGGGAG....................................................331.000.001.00-----------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTCAG.....................................................................261.000.00-----1.00------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTCTCG....................................................................271.000.00-----1.00------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTAGCG.............................................................321.000.001.00-----------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTACAT.............................................................321.000.00---1.00--------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTCCG...................................................................271.000.00-----1.00------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTAACG...............................................................311.000.00--1.00---------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTTTTTTTTACA..............................................................321.000.00------1.00-----------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTTTTTTAT...............................................................321.000.00--1.00---------------------------
............................................................................................................................................................TATTCTTTTTTTTTTTTTTTTTCTCG....................................................................261.000.001.00-----------------------------
.............................................................................................................................................................ATTCTTTTTTTTTTTTTTTTTTTTTTTTGCGGG............................................................331.000.00----1.00-------------------------
...........................................................................................................................................................ATATTCTTTTTTTTTTTTTTTTTTTCGT...................................................................281.000.00--1.00---------------------------
.............................................................................................................................................................................................................CGCCCGGGCTGGAGTGCAGTGT.......................2250.400.40----------------------------0.200.20
.............................................................................................................................................................................................................CGCCCGGGCTGGAGTGCAGTGTAGGG...................2650.200.40-------0.20----------------------

Antisense strand
ACAAGATCAAGTCAACCTTTGTAGATGGATTACTAGCTTGCATGAAAAAGGTAACAAGCTTTACAAAAAGATACTGTGATTAGGACTGTGGTTTTTGTATGAGTTCAGATACATATAGAACCTTTGTAATTTTAGTAAATGATGAAATCGTCTAAATATTCTTTTTTTTTTTTTTTTTTTTTTTTGAGACAGAGTCTTGCACTGTCGCCCGGGCTGGAGTGCAGTGTCAGGATCTCAGCTCACTGCAACC
.......................................................................(((((.(((((.((....((.(((((((((.((....)).))))))))))).)).))))).)))))...((((....))))..................................................................................................
....................................................................69..................................................................................................................185...............................................................
SizePerfect hitTotal NormPerfect NormSRR189780(GSM714640)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR189776(GSM714636)
cell line: HEK293clip variant: CLIPenzymatic . (cell line)		SRR189775(GSM714635)
cell line: HEK293clip variant: CLIPenzymatic . (cell line)		SRR189777(GSM714637)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR189779(GSM714639)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR189778(GSM714638)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR189781(GSM714641)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR095854(SRX039177)
"miRNA were isolated from FirstChoice Human B. (brain)		SRR037943(GSM510481)
293DcrTN. (cell line)		SRR189786SRR553573(SRX182779)
source: Cerebellum. (Cerebellum)		SRR039614(GSM531977)
HBV-infected Liver Tissue. (liver)		SRR189787GSM532886(GSM532886)
G850T. (cervix)		DRR001488(DRX001042)
"Hela short nuclear cell fraction, LNA(+)". (hela)		SRR330908(SRX091746)
tissue: normal skindisease state: normal. (skin)		DRR000555(DRX000313)
"THP-1 whole cell RNA, no treatment". (cell line)		DRR000562(DRX000320)
Isolation of RNA following immunoprecipitatio. (ago3 cell line)		SRR330888(SRX091726)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR039190(GSM494809)
PBMCs were isolated by ficoll gradient from t. (blood)		SRR189783GSM1105753INPUT(GSM1105753)
small RNA sequencing data. (hela)		DRR000558(DRX000316)
"THP-1 whole cell RNA, after 3 day treatment . (cell line)		SRR390725(GSM850204)
small rna immunoprecipitated. (cell line)		SRR330868(SRX091706)
tissue: skin psoriatic involveddisease state:. (skin)		SRR330877(SRX091715)
tissue: skin psoriatic involveddisease state:. (skin)		SRR330902(SRX091740)
tissue: skin psoriatic uninvolveddisease stat. (skin)		GSM956925Paz8D5(GSM956925)
"cell line: HEK293 cell linepassages: 15-20ip. (cell line)		SRR060984(GSM569188)
Human plasma cell [09-001]. (cell line)		SRR038856(GSM458539)
D11. (cell line)
................................................................................................................................................................................................................CCGGGCTGGAGTGCAGTGTC...................... 202.000.00----2.00-------------------------
.........................................................................................................................................................................................................CTGTCGCCCGGGCTGGAGTGCAGTGTCAAGCG................. 321.000.001.00-----------------------------
...........................................................................................................................................................................................................GTCGCCCGGGCTGGAGTCCT........................... 201.000.00----1.00-------------------------
..........................................................................................................................................................................................................TGTCGCCCGGGCTGGAGTGCAGTCC....................... 251.000.001.00-----------------------------
................................................................................................................................................................................................AGTCTTGCACTGTCGCCCGGGCTGCG................................ 261.000.00----1.00-------------------------
.....................................................................................................................................................................................TTTTGAGACAGAGTCTTGCAGAC.............................................. 231.000.00---------1.00--------------------
...............................................................................................................................................................................................................CCCGGGCTGGAGTGCAGTGTCAGGATG................ 271.000.00----1.00-------------------------
...........................................................................................................................................................................................................GTCGCCCGGGCTGGAGTGCAGTCAAG..................... 261.000.001.00-----------------------------
...........................................................................................................................................................................................................GTCGCCCGGGCTGGAGTGCAGTGTTT..................... 261.000.00------------1.00-----------------
...........................................................................................................................................................................................................GTCGCCCGGGCTGGAGTGCAGTGTAT..................... 261.000.00---------1.00--------------------
.........................................................................................................................................................................................................CTGTCGCCCGGGCTGGAGTGCACGAC....................... 261.000.001.00-----------------------------
............................................................................................................................................................AAAAAAAAAAAAAAAAAAAAAAAGAATA.................................................................. 2801.001.00-----------------------1.00------
...........................................................................................................................................................................................................GTCGCCCGGGCTGGAGTGCATA......................... 221.000.00----1.00-------------------------
..................................................................................................................................................................................................TCTTGCACTGTCGCCCGGGCTGGGA............................... 251.000.00----------1.00-------------------
.........................................................................................................................................................................................................CTGTCGCCCGGGCTGGAGTGCAGTGC....................... 261.000.00----1.00-------------------------
...............................................................................................................................................................................................................CCCGGGCTGGAGTGCAGTGTCAAGC.................. 251.000.001.00-----------------------------
...........................................................................................................................................................................................................GTCGCCCGGGCTGGAGTGCAGTT........................ 231.000.00---------1.00--------------------
.........................................................................................................................................................................................GAGACAGAGTCTTGCACTGTCGCCCGGGC.................................... 291.000.001.00-----------------------------
...................................................................................................................................................................................................CTTGCACTGTCGCCCGGGCTTCTC............................... 241.000.00------------1.00-----------------
................................................................................................................................................................................................................CCGGGCTGGAGTGCAGTGTCACTG.................. 241.000.001.00-----------------------------
.........................................................................................................................................................................................................ACACTGCACTCCAGCCCGGGCGACAG....................... 2630.670.670.67-----------------------------
..............................................................................................................................................................................................................TGACACTGCACTCCAGCCCGGGC..................... 2370.140.14----0.14-------------------------