ID: uc004ecz.3_intron_4_0_chrX_77372913_f.3p
GENE: (5)
chrX:77373348-77373597+


(2)
AGO2.ip 		(6)
B-CELL 		(5)
BRAIN 		(3)
BREAST 		(19)
CELL-LINE 		(3)
CERVIX 		(3)
HEART 		(5)
HELA 		(1)
KIDNEY 		(7)
LIVER 		(1)
OTHER 		(2)
RRP40.ip 		(13)
SKIN 		(1)
UTERUS 		(2)
XRN.ip

Sense strand
TTACTCTTTCCCTTTGACCTTGCACTATTAGTTAAAAATCTTATCTTGTACCTAAGTGTTTTTTGTTGCAATGTAGGGATGAAAACCATGATTTCTTGCAGTCCAGAAGTAGAAATATTTTAGTGATAAGGAGCTGGCCTCTAGGACTAGGAGGAATGATAGGGATTGACTAGAATCTGAATGTCTTTGATCTTTTCTAGCTCCATGGTAGGAGTCAATCTGCCACAGAAGGCTGGTGGGTTTTTGATGA
..........................................................(((((((((((..((((((((.............)))))))).....((((((....)))))).)))))))))))....(((((.......)))))........(((((......)))))........................................................................
..........................................................59........................................................................................................................181...................................................................
SizePerfect hitTotal NormPerfect NormDRR001486(DRX001040)
"Hela long cytoplasmic cell fraction, LNA(+)". (hela)		DRR001485(DRX001039)
"Hela long total cell fraction, LNA(+)". (hela)		SRR207110(GSM721072)
Nuclear RNA. (cell line)		SRR033715(GSM497060)
Mantle Cell Lymphoma (Mino122). (B cell)		SRR033727(GSM497072)
HIV-positive DL (HIV412). (B cell)		SRR060168(GSM565978)
5-8F_nucleus. (cell line)		SRR039190(GSM494809)
PBMCs were isolated by ficoll gradient from t. (blood)		SRR033710(GSM497055)
GCB DLBCL (GCB385). (B cell)		SRR039192(GSM494811)
K562 cell line is derived from a CML patient . (cell line)		SRR033721(GSM497066)
Ly3 cell line (Ly3). (B cell)		SRR037944(GSM510482)
293DcrTN_cand5. (cell line)		RoviraIPAgo2(Rovira)
total RNA. (ago2 breast)		SRR029124(GSM416753)
HeLa. (hela)		SRR330861(SRX091699)
tissue: skin psoriatic involveddisease state:. (skin)		SRR060169(GSM565979)
5-8F_cytoplasm. (cell line)		SRR033730(GSM497075)
Lymphoblastic (ALL411). (B cell)		SRR039619(GSM531982)
HBV(+) HCC Tissue Sample 1. (liver)		SRR039623(GSM531986)
HCV(+) HCC Tissue Sample. (liver)		DRR001487(DRX001041)
"Hela long nuclear cell fraction, LNA(+)". (hela)		SRR039611(GSM531974)
Human Normal Liver Tissue Sample 1. (liver)		SRR342894(SRX096790)
small RNA seq of Right atrial tissue. (heart)		SRR189784SRR029054(GSM402329)
MCF7_smallRNAseq. (cell line)		TAX577580(Rovira)
total RNA. (breast)		GSM532876(GSM532876)
G547T. (cervix)		SRR039614(GSM531977)
HBV-infected Liver Tissue. (liver)		SRR039625(GSM531988)
HBV(-) HCV(-) HCC Tissue Sample. (liver)		SRR039620(GSM531983)
HBV(+) Adjacent Tissue Sample 2. (liver)		GSM450607(GSM450607)
miRNA sequencing raw reads from post-mortem s. (brain)		SRR330913(SRX091751)
tissue: normal skindisease state: normal. (skin)		SRR553575(SRX182781)
source: Kidney. (Kidney)		GSM450601(GSM450601)
miRNA sequencing raw reads from post-mortem s. (brain)		GSM532883(GSM532883)
G871N. (cervix)		SRR207112(GSM721074)
RRP40 knockdown. (RRP40 cell line)		SRR039618(GSM531981)
HBV(+) Side Tissue Sample 1. (liver)		SRR330903(SRX091741)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR094131(GSM651907)
small RNA(18-35nt)small RNA deep sequencing o. (uterus)		SRR207118(GSM721080)
RRP40 knockdown. (RRP40 cell line)		SRR444049(SRX128897)
Sample 9cDNABarcode: AF-PP-334: ACG CTC TTC C. (skin)		SRR038858(GSM458541)
MEL202. (cell line)		SRR189779(GSM714639)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR330896(SRX091734)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR189780(GSM714640)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR330901(SRX091739)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR330874(SRX091712)
tissue: skin psoriatic involveddisease state:. (skin)		SRR207115(GSM721077)
XRN1&2 knockdown. (XRN1/XRN2 cell line)		SRR330868(SRX091706)
tissue: skin psoriatic involveddisease state:. (skin)		DRR001483(DRX001037)
"Hela long cytoplasmic cell fraction, control. (hela)		GSM450600(GSM450600)
miRNA sequencing raw reads from post-mortem s. (brain)		SRR330858(SRX091696)
tissue: skin psoriatic involveddisease state:. (skin)		GSM956925Ago2PAZ(GSM956925)
"cell line: HEK293 cell linepassages: 15-20ip. (ago2 cell line)		SRR330866(SRX091704)
tissue: skin psoriatic involveddisease state:. (skin)		GSM450597(GSM450597)
miRNA sequencing raw reads from post-mortem s. (brain)		GSM956925Paz8D5(GSM956925)
"cell line: HEK293 cell linepassages: 15-20ip. (cell line)		SRR330876(SRX091714)
tissue: skin psoriatic involveddisease state:. (skin)		SRR207119(GSM721081)
XRN1&2 knockdown. (XRN1/XRN2 cell line)		SRR033711(GSM497056)
GCB DLBCL (GCB110). (B cell)		SRR191553(GSM715663)
96genomic small RNA (size selected RNA from t. (breast)		SRR189781(GSM714641)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR038852(GSM458535)
QF1160MB. (cell line)		SRR040010(GSM532895)
G529N. (cervix)		SRR330904(SRX091742)
tissue: normal skindisease state: normal. (skin)		SRR207116(GSM721078)
Nuclear RNA. (cell line)		GSM450610(GSM450610)
miRNA sequencing raw reads from post-mortem s. (brain)		SRR330859(SRX091697)
tissue: skin psoriatic involveddisease state:. (skin)		DRR000555(DRX000313)
"THP-1 whole cell RNA, no treatment". (cell line)		SRR342898(SRX096794)
small RNA seq of Right atrial tissue. (heart)		SRR342901(SRX096797)
small RNA seq of Left atrial tissue. (heart)
......................................................................................................................................TGGCCTCTAGGACTAGGAGGAATGATAGGGATTGACTAGAATCTGAATGTCTTTGATCTTTTCTA...................................................65119.0019.006.0010.00----------------2.00----------------------------1.00--------------------
...............................................................................................................................................................TAGGGATTGACTAGAATC.........................................................................1819.009.00--9.00-----------------------------------------------------------------
...................................................................................................................................AGCTGGCCTCTAGGACTAGGAGGAATGATAGGGATTGACTAGAATCTGAATGTCTTTGATCTTTTCTAG..................................................6919.009.003.006.00------------------------------------------------------------------
.................................................................................................................................................................................................................................CAGAAGGCTGGTGGGTTTTTG....2118.008.00-----3.00-1.00-------2.00---1.00-------1.00----------------------------------------
......................................................................................................................................TGGCCTCTAGGACTAGGAGGAATGATAGGGATTGACTAGAATCTGAATGTCTTTGATCTTTTCTAG..................................................6617.007.007.00-------------------------------------------------------------------
..................................................................................................................................GAGCTGGCCTCTAGGACTAGGAGGAATGATAGGGATTGACTAGAATCTGAATGTCTTTGATCTTTTCTAG..................................................7017.007.007.00-------------------------------------------------------------------
................................................................................................................................................................................................................................ACAGAAGGCTGGTGGGTT........1816.006.00---1.00--1.002.00-1.00-------1.00--------------------------------------------------
...............................................................................................................................................................TAGGGATTGACTAGAAT..........................................................................1716.006.00--6.00-----------------------------------------------------------------
.................................................................................................................................................................................................................................CAGAAGGCTGGTGGGTTTT......1916.006.00---2.001.00-1.00-------------------1.00-------------------------1.00---------------
..................................................................................................................................................................................................................................AGAAGGCTGGTGGGTTTT......1815.005.00---3.00----------------------------------1.001.00----------------------------
.................................................................................................................................................................................................................................CAGAAGGCTGGTGGGTTTTT.....2014.004.00---------1.00----1.00-1.001.00--------------------------------------------------
..................................................................................................................................................................................................................................AGAAGGCTGGTGGGTTTTT.....1914.004.00---1.00-----1.00--------------1.00-------------------------------1.00-----------
............................................................TTTTGTTGCAATGTAGGGATGAAAACCA..................................................................................................................................................................2813.003.00----------3.00---------------------------------------------------------
...................................................................................................................................................................................................................................GAAGGCTGGTGGGTTTTT.....1813.003.00----3.00---------------------------------------------------------------
.................................................................................................................................................................................................................................CAGAAGGCTGGTGGGTTTTTGAA..232.000.00------1.00-------1.00-----------------------------------------------------
..................................................................................................................................................................................................................................AGAAGGCTGGTGGGTTTTTG....2012.002.00---1.00--1.00-------------------------------------------------------------
.................................................................................................................................................................................................................................CAGAAGGCTGGTGGGTTT.......1812.002.00---1.001.00---------------------------------------------------------------
.................................................................................................................................................................................................................................CAGAAGGCTGGTGGGTTTTTGAAA.242.000.00--------1.00--------------------------------------------------1.00--------
....................................................................................................................................................................................................................................AAGGCTGGTGGGTTTTTGA...1912.002.00--------------------------------1.00------------------------1.00----------
...............................................................................................................................................................................................................................CACAGAAGGCTGGTGGGTT........1911.001.00---1.00----------------------------------------------------------------
...............................................................TGTTGCAATGTAGGGATG.........................................................................................................................................................................1811.001.00----------------------------------------1.00---------------------------
............................................................................................................................................................................................................ATGGTAGGAGTCAATCTGCCACAGAAGGCTGGTGG...........3511.001.00-----------1.00--------------------------------------------------------
........................................................................................................................................................................................................CTCCATGGTAGGAGTCAATCTGC...........................2311.001.00-----------------------------------------------------1.00--------------
...............................................................................................................................................................................................................GTAGGAGTCAATCTGCCACAGAAGGCTGG..............2911.001.00--------------------------------------------1.00-----------------------
..................................................................................................................................................................................................................................AGAAGGCTGGTGGGTTTTTGAT..2211.001.00-------------------------1.00------------------------------------------
...............................................................................................................................................................................................................................CACAGAAGGCTGGTGGGTTTT......2111.001.00----1.00---------------------------------------------------------------
.....................................................................................................................................................................................................................................AGGCTGGTGGGTTTTTTATG.201.000.00------------1.00-------------------------------------------------------
....................................................................................................................................................AGGAGGAATGATAGGTAA....................................................................................181.000.00---1.00----------------------------------------------------------------
...............................................................................................................................................................................................................GTAGGAGTCAATCTGCCACAGAAGGCT................2711.001.00---------------------------------------------1.00----------------------
......................................................................................................................................................................TGACTAGAATCTGAATGTCTTTGATCTTTTCTAAGA................................................361.000.00-------------------------------------------------------1.00------------
.....................................................................................................................................................................................................................................AGGCTGGTGGGTTTTTGA...1811.001.00-----------------------------1.00--------------------------------------
...................................................................................................................................................................................................................................GAAGGCTGGTGGGTTTTA.....181.000.00------1.00-------------------------------------------------------------
...........................................................................................................................................................................................................CATGGTAGGAGTCAATCTGCCACAGAAGGC.................3011.001.00----------------------------------------------1.00---------------------
........................................................................................................................................................GGAATGATAGGGATTGACTAGA............................................................................2211.001.00---------------------------------------------------1.00----------------
..............................................................................................................................................................................................................................CCACAGAAGGCTGGTGGGTTTTTGA...2511.001.00---1.00----------------------------------------------------------------
................................................................GTTGCAATGTAGGGATGAAAACCATGATTTC...........................................................................................................................................................3111.001.00-------------1.00------------------------------------------------------
.................................................................................................................................................................................................................................CAGAAGGCTGGTGGGTTTTTGATG.2411.001.00-----1.00--------------------------------------------------------------
.....................GCACTATTAGTTAAAAAAAAA................................................................................................................................................................................................................211.000.00--------1.00-----------------------------------------------------------
................................................................................................................................................................................................................................ACAGAAGGCTGGTGGGT.........1711.001.00---------------------------------1.00----------------------------------
................................................................................................................................................................................................................................ACAGAAGGCTGGTGGGTTTTTGAT..2411.001.00--------1.00-----------------------------------------------------------
..................................................................................................................................................................................................................................AGAAGGCTGGTGGGTTTTTGATGA2411.001.00----------------1.00---------------------------------------------------
.................................................................................................................................................................................................................................CAGAAGGCTGGTGGGTTGTT.....201.000.00-----1.00--------------------------------------------------------------
................................................................................................................................................................AGGGATTGACTAGAATCTGAATGTCTT...............................................................2711.001.00-----1.00--------------------------------------------------------------
....................................................................................ACCATGATTTCTTGCAGTCCAGAAGTAGA.........................................................................................................................................2911.001.00-------------1.00------------------------------------------------------
....................................................................................................................................................AGGAGGAATGATAGGGATTGACTA..............................................................................2411.001.00------------------------------------1.00-------------------------------
....................................................................................................................................................................................................................AGTCAATCTGCCACAGAAG...................1921.001.00-----------------------------------------1.00--------------------------
....................................................................................................................................................................................................................................AAGGCTGGTGGGTTTGTT....181.000.00----------------------------1.00---------------------------------------
...........................................................................................................................................................................................................CATGGTAGGAGTCAATCTGCCACAGAAGGCTGGTGG...........3611.001.00-----------1.00--------------------------------------------------------
............................................................................................................................................................TGATAGGGATTGACTAGAAT..........................................................................2011.001.00-------------------1.00------------------------------------------------
...............................................................................................................................AAGGAGCTGGCCTCTTCAG........................................................................................................191.000.00------------------------------------------1.00-------------------------
..............................................................................................................................................AGGACTAGGAGGAATGATAGGGATTGACTAGA............................................................................3211.001.00-------------------------------------1.00------------------------------
...............................................................................................................................................................................................................................CACAGAAGGCTGGTGGGTTTTTGAT..2511.001.00--------------------1.00-----------------------------------------------
..................................................................................................................................................................................................................................AGAAGGCTGGTGGGTTTA......181.000.00----------------------------------1.00---------------------------------
........................................................................................................................................................GGAATGATAGGGATTGACTAGAATCTGAAT....................................................................3011.001.00------------------------------------------------------1.00-------------
........................................................................................................................................................GGAATGATAGGGATTGACTAGAATCTGAATG...................................................................3111.001.00-------------------------------------------1.00------------------------
......................................................................................................................................................................................................................TCAATCTGCCACAGAAGGCTGGTGGGAC........281.000.00----------------------------------------------------------1.00---------
............................................................................................................................................................................................................ATGGTAGGAGTCAATCTGCC..........................2011.001.00--------------------------------------------------1.00-----------------
.....................................................................................................................................CTGGCCTCTAGGACTAGGAG.................................................................................................2011.001.00-----------------------------------1.00--------------------------------
...............................................GTACCTAAGTGTTTTTTGTTGCAATGTAGG.............................................................................................................................................................................3011.001.00-------------------------------------------------1.00------------------
.............................................................................................................................................................GATAGGGATTGACTAGAATCT........................................................................2111.001.00------------------------------1.00-------------------------------------
.....................................................................................................................................................................................................................................AGGCTGGTGGGTTTTTGATGA2111.001.00------------1.00-------------------------------------------------------
.........................................................................................................................................................................................................................ATCTGCCACAGAAGGCTGG..............1920.500.50------------------------------------------------------------0.50-------
.....................................................................................................................................................................................................................GTCAATCTGCCACAGAAGGCTG...............2220.500.50--------------------------------------------------------------0.50-----
........................................................................................................................................................................................................................AATCTGCCACAGAAGGCT................1820.500.50--------------------0.50-----------------------------------------------
..........................................................................................................................................................................................................................TCTGCCACAGAAGGCTGGT.............1930.330.33----------------------------------------------------------------0.33---
.............................................................................................................................................................................................................................GCCACAGAAGGCTGGTGG...........1830.330.33---------------------------------------------------------------0.33----
................................................................................................................................................................AGGGATTGACTAGAAT..........................................................................1640.250.25-----------------------------------------------------------------0.25--
...........................................................................................................................................TCTAGGACTAGGAGG................................................................................................1560.170.17------------------------------------------------------------------0.17-
...............................................................................................................................................................................................................................CACAGAAGGCTGGTGG...........1690.110.11-------------------------------------------------------------------0.11

Antisense strand
TTACTCTTTCCCTTTGACCTTGCACTATTAGTTAAAAATCTTATCTTGTACCTAAGTGTTTTTTGTTGCAATGTAGGGATGAAAACCATGATTTCTTGCAGTCCAGAAGTAGAAATATTTTAGTGATAAGGAGCTGGCCTCTAGGACTAGGAGGAATGATAGGGATTGACTAGAATCTGAATGTCTTTGATCTTTTCTAGCTCCATGGTAGGAGTCAATCTGCCACAGAAGGCTGGTGGGTTTTTGATGA
..........................................................(((((((((((..((((((((.............)))))))).....((((((....)))))).)))))))))))....(((((.......)))))........(((((......)))))........................................................................
..........................................................59........................................................................................................................181...................................................................
SizePerfect hitTotal NormPerfect NormDRR001486(DRX001040)
"Hela long cytoplasmic cell fraction, LNA(+)". (hela)		DRR001485(DRX001039)
"Hela long total cell fraction, LNA(+)". (hela)		SRR207110(GSM721072)
Nuclear RNA. (cell line)		SRR033715(GSM497060)
Mantle Cell Lymphoma (Mino122). (B cell)		SRR033727(GSM497072)
HIV-positive DL (HIV412). (B cell)		SRR060168(GSM565978)
5-8F_nucleus. (cell line)		SRR039190(GSM494809)
PBMCs were isolated by ficoll gradient from t. (blood)		SRR033710(GSM497055)
GCB DLBCL (GCB385). (B cell)		SRR039192(GSM494811)
K562 cell line is derived from a CML patient . (cell line)		SRR033721(GSM497066)
Ly3 cell line (Ly3). (B cell)		SRR037944(GSM510482)
293DcrTN_cand5. (cell line)		RoviraIPAgo2(Rovira)
total RNA. (ago2 breast)		SRR029124(GSM416753)
HeLa. (hela)		SRR330861(SRX091699)
tissue: skin psoriatic involveddisease state:. (skin)		SRR060169(GSM565979)
5-8F_cytoplasm. (cell line)		SRR033730(GSM497075)
Lymphoblastic (ALL411). (B cell)		SRR039619(GSM531982)
HBV(+) HCC Tissue Sample 1. (liver)		SRR039623(GSM531986)
HCV(+) HCC Tissue Sample. (liver)		DRR001487(DRX001041)
"Hela long nuclear cell fraction, LNA(+)". (hela)		SRR039611(GSM531974)
Human Normal Liver Tissue Sample 1. (liver)		SRR342894(SRX096790)
small RNA seq of Right atrial tissue. (heart)		SRR189784SRR029054(GSM402329)
MCF7_smallRNAseq. (cell line)		TAX577580(Rovira)
total RNA. (breast)		GSM532876(GSM532876)
G547T. (cervix)		SRR039614(GSM531977)
HBV-infected Liver Tissue. (liver)		SRR039625(GSM531988)
HBV(-) HCV(-) HCC Tissue Sample. (liver)		SRR039620(GSM531983)
HBV(+) Adjacent Tissue Sample 2. (liver)		GSM450607(GSM450607)
miRNA sequencing raw reads from post-mortem s. (brain)		SRR330913(SRX091751)
tissue: normal skindisease state: normal. (skin)		SRR553575(SRX182781)
source: Kidney. (Kidney)		GSM450601(GSM450601)
miRNA sequencing raw reads from post-mortem s. (brain)		GSM532883(GSM532883)
G871N. (cervix)		SRR207112(GSM721074)
RRP40 knockdown. (RRP40 cell line)		SRR039618(GSM531981)
HBV(+) Side Tissue Sample 1. (liver)		SRR330903(SRX091741)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR094131(GSM651907)
small RNA(18-35nt)small RNA deep sequencing o. (uterus)		SRR207118(GSM721080)
RRP40 knockdown. (RRP40 cell line)		SRR444049(SRX128897)
Sample 9cDNABarcode: AF-PP-334: ACG CTC TTC C. (skin)		SRR038858(GSM458541)
MEL202. (cell line)		SRR189779(GSM714639)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR330896(SRX091734)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR189780(GSM714640)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR330901(SRX091739)
tissue: skin psoriatic uninvolveddisease stat. (skin)		SRR330874(SRX091712)
tissue: skin psoriatic involveddisease state:. (skin)		SRR207115(GSM721077)
XRN1&2 knockdown. (XRN1/XRN2 cell line)		SRR330868(SRX091706)
tissue: skin psoriatic involveddisease state:. (skin)		DRR001483(DRX001037)
"Hela long cytoplasmic cell fraction, control. (hela)		GSM450600(GSM450600)
miRNA sequencing raw reads from post-mortem s. (brain)		SRR330858(SRX091696)
tissue: skin psoriatic involveddisease state:. (skin)		GSM956925Ago2PAZ(GSM956925)
"cell line: HEK293 cell linepassages: 15-20ip. (ago2 cell line)		SRR330866(SRX091704)
tissue: skin psoriatic involveddisease state:. (skin)		GSM450597(GSM450597)
miRNA sequencing raw reads from post-mortem s. (brain)		GSM956925Paz8D5(GSM956925)
"cell line: HEK293 cell linepassages: 15-20ip. (cell line)		SRR330876(SRX091714)
tissue: skin psoriatic involveddisease state:. (skin)		SRR207119(GSM721081)
XRN1&2 knockdown. (XRN1/XRN2 cell line)		SRR033711(GSM497056)
GCB DLBCL (GCB110). (B cell)		SRR191553(GSM715663)
96genomic small RNA (size selected RNA from t. (breast)		SRR189781(GSM714641)
cell line: HEK293clip variant: PAR-CLIPenzyma. (cell line)		SRR038852(GSM458535)
QF1160MB. (cell line)		SRR040010(GSM532895)
G529N. (cervix)		SRR330904(SRX091742)
tissue: normal skindisease state: normal. (skin)		SRR207116(GSM721078)
Nuclear RNA. (cell line)		GSM450610(GSM450610)
miRNA sequencing raw reads from post-mortem s. (brain)		SRR330859(SRX091697)
tissue: skin psoriatic involveddisease state:. (skin)		DRR000555(DRX000313)
"THP-1 whole cell RNA, no treatment". (cell line)		SRR342898(SRX096794)
small RNA seq of Right atrial tissue. (heart)		SRR342901(SRX096797)
small RNA seq of Left atrial tissue. (heart)
..................................................................................AAACCATGATTTCTTGCAGGCT.................................................................................................................................................. 222.000.00-------------------------------1.00----------------1.00-------------------
............TTTGACCTTGCACTAAGTG........................................................................................................................................................................................................................... 191.000.00---------------------1.00----------------------------------------------
......................................................................................................CCAGAAGTAGAAATATTTGA................................................................................................................................ 201.000.00----------------------1.00---------------------------------------------
..................................................................................AAACCATGATTTCTTGCAAGTT.................................................................................................................................................. 221.000.00-----------------------1.00--------------------------------------------
.................................................................................................TATTTCTACTTCTGGACTGC..................................................................................................................................... 2020.500.50-------------------------------------------------------------0.50------