dme-mir-iab-8- Best orthologs

dme-mir-iab-8	chr3R:12681997-12682064 -	Known_mirbase
[View on miRBase]	[View on UCSC Genome Browser {Cornell Mirror}]	intergenic

Legend:

   mature strand at position 12682041-12682059
   star strand at position 12682000-12682021
   mutations
   read mismatches

20-way Fast Statistical Aligner (FSA) re-alignment

Visually pleasing best hits from LASTZ and MAF

Species	Coordinate	Source	Confidence	Alignment
dm3	chr3R:12681982-12682079 -		1	GTTGTTAGTGCTCTCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCG
droSim1	chr3R:8791202-8791299 +	LASTZ	1	GTTGCTCGTGCTATCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCG
droSec1	super_12:1472230-1472327 +	LASTZ	1	GTTGCTCGTGCTATCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCG
droYak2	chr3R:11708953-11709050 +	LASTZ	1	GTTGCTCGTGCTTTCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCG
droEre2	scaffold_4770:8952402-8952499 +	LASTZ	1	GTTGCTCGTGCTTTCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCG
droEug1	scf7180000409804:92907-93004 -	LASTZ	4	GTTGCTCGTGCTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACACAATCA
droBia1	scf7180000299102:622778-622875 -	LASTZ	4	GTTGCTCGTGCTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCA
droTak1	scf7180000411269:86577-86674 -	LASTZ	4	GTTGCTCGTGCTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCA
droEle1	scf7180000486474:1729438-1729535 -	LASTZ	4	GTTGCTCGTGCTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCA
droRho1	scf7180000759657:23990-24087 -	LASTZ	4	GTTGCTCGTGCTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCA
droFic1	scf7180000452523:122363-122460 -	LASTZ	4	GTTGCTCGTGCTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACACAATCA
droKik1	scf7180000302313:151175-151272 -	LASTZ	4	GTTGCTTGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAGAGTCA
droAna3	scaffold_13340:7215188-7215285 +	LASTZ	1	GTTGAAGGTGTTACCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAAAATCA
droBip1	scf7180000396708:1638868-1638965 -	LASTZ	4	GTTGAAAGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAAAATCA
dp4	chr2:17671753-17671849 -	LASTZ	1	GTTGCTGGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTCGCACAGGTTC-
droPer1	super_0:11526559-11526655 -	LASTZ	1	GTTGCTGGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTCGCACAGGTTC-
droWil1	scaffold_181130:4905585-4905682 +	LASTZ	1	GTTGTTGGTGTTTCATCGTGTTACGTATACTGAAGGTATACCGGATAGCATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAGATTCA
droVir3	scaffold_12855:414691-414788 +	LASTZ	1	GTCGCTGGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAGGCTCT
droMoj3	scaffold_6540:1943729-1943826 -	LASTZ	1	GTTGCTGGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAGGCTCA
droGri2	scaffold_14906:12815109-12815201 -	LASTZ	1	GTTGCTGGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAG-----

Legend

0- The ortholog is absent. A better synthenic sequence candidate could not be found.
1- Reads w/ good pileup on mature and star strand & folds into hairpin structure.
2- Reads w/ good pileup on mature and star strand BUT does not fold into hairpin structure.
3- Sporadic reads & folds into a hairpin structure.
4- No reads but folds into a hairpin structure.
5- Ortholog is confident but miRNA appears absent (no reads and does not fold into a hairpin)

Re-alignment after removal of non-confident orthologs

Species	Alignment
dm3	GTTGTTAGTGCTCTCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCG
droSim1	GTTGCTCGTGCTATCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCG
droSec1	GTTGCTCGTGCTATCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCG
droYak2	GTTGCTCGTGCTTTCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCG
droEre2	GTTGCTCGTGCTTTCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCG
droEug1	GTTGCTCGTGCTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACACAATCA
droBia1	GTTGCTCGTGCTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCA
droTak1	GTTGCTCGTGCTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCA
droEle1	GTTGCTCGTGCTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCA
droRho1	GTTGCTCGTGCTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCA
droFic1	GTTGCTCGTGCTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACACAATCA
droKik1	GTTGCTTGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAGAGTCA
droAna3	GTTGAAGGTGTTACCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAAAATCA
droBip1	GTTGAAAGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAAAATCA
dp4	GTTGCTGGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTCGCACAGGTTC-
droPer1	GTTGCTGGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTCGCACAGGTTC-
droWil1	GTTGTTGGTGTTTCATCGTGTTACGTATACTGAAGGTATACCGGATAGCATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAGATTCA
droVir3	GTCGCTGGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAGGCTCT
droMoj3	GTTGCTGGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAGGCTCA
droGri2	GTTGCTGGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAG-----

20-way Secondary Structure

	dm3	droSim1	droSec1	droYak2	droEre2	droEug1	droBia1	droTak1	droEle1	droRho1	droFic1	droKik1	droAna3	droBip1	dp4	droPer1	droWil1	droVir3	droMoj3	droGri2
RNAfold Image
UNAFold Image

Small RNA-seq read pileup

Species

Alignment

droSim1

GTTGCTCGTGCTATCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCG	Size	Hit Count	Total Norm	V044 Embryo	M023 Head	M024 Male body	M025 Embryo
....................TTACGTATACTGAAGGTATACC........................................................	22	2	19.50	13.50	0.00	3.50	2.50
....................TTACGTATACTGAAGGTAT...........................................................	19	2	14.50	1.50	0.50	12.00	0.50
....................TTACGTATACTGAAGGTATACCGGA.....................................................	25	2	5.50	2.00	0.00	1.00	2.50
....................TTACGTATACTGAAGGTATAC.........................................................	21	2	5.00	3.00	0.00	2.00	0.00
....................TTACGTATACTGAAGGTATA..........................................................	20	2	4.50	2.50	0.00	1.00	1.00
......................ACGTATACTGAATGTATTC.........................................................	19	4	1.75	0.00	0.00	0.00	1.75
.TTGCTCGTGCTATCTCGTG..............................................................................	19	2	2.50	1.00	0.00	0.00	1.50
....................TTACGTATACTGAAGGTATACCGG......................................................	24	2	1.50	1.50	0.00	0.00	0.00
GTTGCTCGTGCTATCTCGTG..............................................................................	20	2	1.00	0.00	0.00	0.00	1.00
..........................................................GGATACATTCAGTATACGTTTA..................	22	2	1.00	1.00	0.00	0.00	0.00
......................ACGTATACTGAATGTATCCCG.......................................................	21	4	0.50	0.25	0.00	0.00	0.25
....................TTACGTATACTGAAGGTATACCGGG.....................................................	25	2	1.00	0.00	0.00	0.00	1.00
....................TTACGTATACTGAAGGTATACCG.......................................................	23	2	1.00	0.00	0.00	0.00	1.00
....................TTACGTATACTGAAGGTATACCA.......................................................	23	2	0.50	0.00	0.00	0.00	0.50
....................TTACGTATACTGAAGGGATTCC........................................................	22	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAGGGTAT...........................................................	19	2	0.50	0.00	0.00	0.50	0.00
....................TTACGTATACTGAAGGTAC...........................................................	19	2	0.50	0.00	0.00	0.50	0.00
...........................................GATAGGATACACTCAGGATACATTCA.............................	26	2	0.50	0.00	0.00	0.50	0.00
.............................................TAGGATACACTCAGGATACATTCAGT...........................	26	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCGGT.....................................................	25	2	0.50	0.00	0.00	0.50	0.00
...................GTTACGTATACTGAAGGTATACCGGA.....................................................	26	2	0.50	0.00	0.00	0.00	0.50
....................TTACGTATACTGAAGGTATACCGGAT....................................................	26	2	0.50	0.00	0.00	0.50	0.00
....................TTACGTATACTGAATGTATA..........................................................	20	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGACGGTCTA..........................................................	20	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGGATACCGGA.....................................................	25	2	0.50	0.00	0.00	0.00	0.50
....................TTACGTATACTGAAGGTATAAC........................................................	22	2	0.50	0.50	0.00	0.00	0.00
.....................TACGTATACTGAAGGTATACCGGA.....................................................	24	2	0.50	0.00	0.00	0.50	0.00
.........................................................AGGATACATTCAGTATACGTTTA..................	23	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATT..........................................................	20	2	0.50	0.00	0.00	0.50	0.00
....................TTACGTATACTGGAGGTATACC........................................................	22	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGAAT...........................................................	19	2	0.50	0.00	0.00	0.50	0.00
....................TTACGxxTACTGAAGGTATACCG.......................................................	21	2	0.50	0.00	0.00	0.00	0.50
....................TTACGTATACTGAAGGTATACT........................................................	22	2	0.50	0.50	0.00	0.00	0.00

droSec1

GTTGCTCGTGCTATCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCG	Size	Hit Count	Total Norm	V113 Male body	V114 Embryo	V115 Head
....................TTACGTATACTGAAGGTAT...........................................................	19	2	67.00	47.00	6.50	13.50
....................TTACGTATACTGAAGGTATACC........................................................	22	2	25.50	8.00	17.00	0.50
....................TTACGTATACTGAAGGTATA..........................................................	20	2	18.00	10.50	5.00	2.50
....................TTACGTATACTGAAGGTATACCGG......................................................	24	2	9.00	0.50	8.50	0.00
....................TTACGTATACTGAAGGTATAC.........................................................	21	2	8.00	5.00	1.50	1.50
....................TTACGTATACTGAAGGTA............................................................	18	2	7.50	0.00	6.50	1.00
....................TTACGTATACTGAAGGTATACCG.......................................................	23	2	7.50	1.00	6.50	0.00
....................TTACGTATACTGAAGGTATACCGGA.....................................................	25	2	5.50	1.00	4.50	0.00
....................TTACGTATACTGAAGGTATACCGT......................................................	24	2	4.50	3.50	1.00	0.00
....................TTACGTATACTGAAGGTATACCGGT.....................................................	25	2	3.50	1.00	1.50	1.00
.........................................................AGGATACATTCAGTATACGTTTA..................	23	2	3.00	1.50	1.50	0.00
....................TTACGTATACTGAAGGTATAA.........................................................	21	2	2.00	2.00	0.00	0.00
..................TGTTACGTATACTGAAGGTAT...........................................................	21	2	2.00	1.00	1.00	0.00
.........................................................AGGATACATTCAGTATACGTTT...................	22	2	2.00	0.50	1.00	0.50
.TTGCTCGTGCTATCTCGTG..............................................................................	19	2	1.50	0.00	1.50	0.00
..........................................................GGATACATTCAGTATACGTTTA..................	22	2	1.00	0.50	0.50	0.00
....................TTACGTATACTGAAGGTxTACCGG......................................................	23	2	1.00	0.00	1.00	0.00
....................TTACGTATACTGAAGGTATT..........................................................	20	2	1.00	1.00	0.00	0.00
..........................ATACTGAAGGTATACCGG......................................................	18	2	1.00	0.00	1.00	0.00
....................TTAGGTATACTGAAGGTATACC........................................................	22	2	0.50	0.00	0.50	0.00
....................TTACGTATACTGAAGGTAA...........................................................	19	2	0.50	0.50	0.00	0.00
GTTGCTCGTGCTATCTCGTG..............................................................................	20	2	0.50	0.00	0.50	0.00
....................TTACGTATACTGAAGGTATATC........................................................	22	2	0.50	0.00	0.50	0.00
....................TTACGTATATTGAAGGTATACCGG......................................................	24	2	0.50	0.00	0.50	0.00
....................TTACCTATACTGAAGGTATACCGG......................................................	24	2	0.50	0.00	0.50	0.00
....................TTACGTATACTGAAGGGAT...........................................................	19	2	0.50	0.50	0.00	0.00
.....................TACGTATACTGAAGGTATACCGGT.....................................................	24	2	0.50	0.50	0.00	0.00
...............................................................CATTCAGTATACGTTTAA.................	18	2	0.50	0.00	0.50	0.00
....................TTACGTATCCTGAAGGTATA..........................................................	20	2	0.50	0.50	0.00	0.00
....................TTACGTATACTGAAGGTATACA........................................................	22	2	0.50	0.00	0.00	0.50
....................TTAGGTATACTGAAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00
....................TTACGTATACTGTAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00
.......................CGTATACTGAAGGTATACCGGA.....................................................	22	2	0.50	0.00	0.50	0.00
....................TTACGTATACTGGAGGTATACCGGA.....................................................	25	2	0.50	0.00	0.50	0.00
....................TTACGTATACTGAAGGTATACCGTA.....................................................	25	2	0.50	0.00	0.50	0.00
....................TTACGTATACTGAGGGTATACC........................................................	22	2	0.50	0.50	0.00	0.00
....................TTACGTATACTGAAGGTATACCGTT.....................................................	25	2	0.50	0.50	0.00	0.00
.....................TACGTATACTGAAGGTATACCGGA.....................................................	24	2	0.50	0.00	0.50	0.00
.....................TACGTxTACTGAAGGTATACCGG......................................................	22	2	0.50	0.00	0.50	0.00
....................TTACGTATACTGAAGGTATATCGG......................................................	24	2	0.50	0.00	0.50	0.00
....................TTACGTATACTGAAGGTATACCGGATA...................................................	27	2	0.50	0.00	0.50	0.00
....................TTACGTACACTGAAGGTATA..........................................................	20	2	0.50	0.50	0.00	0.00
....................TTACGTATACTGAAGGCATACCGG......................................................	24	2	0.50	0.00	0.00	0.50
....................TTACGTATACTGAAGGCATA..........................................................	20	2	0.50	0.00	0.50	0.00
....................TTACGTATACTGAAGGTATACT........................................................	22	2	0.50	0.00	0.50	0.00
...................GTTACGGATGCTGAAGGT.............................................................	18	2	0.50	0.00	0.00	0.50
....................GTTCGTATACTGAAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00

droYak2

GTTGCTCGTGCTTTCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCG	Size	Hit Count	Total Norm	V046 Embryo	V052 Head	V120 Male body	V058 Head	M026 Head
....................TTACGTATACTGAAGGTAT...........................................................	19	4	15.75	0.25	1.00	13.25	1.25	0.00
....................TTACGTATACTGAAGGTATA..........................................................	20	4	5.00	3.50	0.00	1.50	0.00	0.00
....................TTACGTATACTGAAGGTATAC.........................................................	21	4	2.00	1.50	0.00	0.50	0.00	0.00
....................TTACGTATACTGAAGGTATACC........................................................	22	4	1.50	0.75	0.25	0.50	0.00	0.00
....................TTACGTATACTGAAGGTATACCGT......................................................	24	4	1.25	0.00	0.00	1.25	0.00	0.00
....................TTACGTATACTGAAGGTATACCGG......................................................	24	4	1.25	1.25	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCG.......................................................	23	4	0.75	0.25	0.25	0.25	0.00	0.00
....................TTACGTATACTGAAGGTATACCGGA.....................................................	25	4	0.50	0.50	0.00	0.00	0.00	0.00
.........................................................AGGATACATTCAGTATACGTTTA..................	23	4	0.50	0.50	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAGCTAT...........................................................	19	4	0.25	0.00	0.00	0.00	0.25	0.00
....................TTAGGTATACTGAAGGTAT...........................................................	19	4	0.25	0.00	0.00	0.25	0.00	0.00
....................TTACGTATACTGCAGGTATACCGGT.....................................................	25	4	0.25	0.25	0.00	0.00	0.00	0.00
..............................................................................AACGAGGTCGCAGAGAAT..	18	2	0.50	0.00	0.00	0.50	0.00	0.00
...................GTTACGTATACTGAAGGTAT...........................................................	20	4	0.25	0.00	0.00	0.25	0.00	0.00
....................TTACGTATACTGACGGTATA..........................................................	20	4	0.25	0.25	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATT..........................................................	20	4	0.25	0.00	0.00	0.25	0.00	0.00
....................TTACGTATACTGAAGGTATACCGGC.....................................................	25	4	0.25	0.25	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCGGT.....................................................	25	4	0.25	0.00	0.00	0.25	0.00	0.00

droEre2

GTTGCTCGTGCTTTCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCG	Size	Hit Count	Total Norm	V040 Embryo	V060 Head	V047 Embryo	V108 Head
....................TTACGTATACTGAAGGTAT...........................................................	19	2	44.50	2.00	2.00	0.00	40.50
....................TTACGTATACTGAAGGTATA..........................................................	20	2	15.50	8.50	0.00	0.00	7.00
....................TTACGTATACTGAAGGTATACC........................................................	22	2	12.00	4.50	0.50	0.00	7.00
....................TTACGTATACTGAAGGTATAC.........................................................	21	2	6.50	1.50	1.00	0.00	4.00
....................TTACGTATACTGAAGGTA............................................................	18	2	4.00	0.00	0.00	0.00	4.00
.TTGCTCGTGCTTTCTCGTG..............................................................................	19	2	1.00	0.00	0.00	0.00	1.00
.........................................................AGGATACATTCAGTATACGTTT...................	22	2	1.00	0.50	0.50	0.00	0.00
....................TTACGTATACTGAAGGAAGA..........................................................	20	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTCAACGTATAC.........................................................	21	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACCGAATGTATA..........................................................	20	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGCAT...........................................................	19	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATCCTGAAGGTAT...........................................................	19	2	0.50	0.00	0.00	0.00	0.50
....................TTACGTATACTGAAGGGATACCGG......................................................	24	2	0.50	0.50	0.00	0.00	0.00
.............................................TAGGATACACTCAGGATACATTCA.............................	24	2	0.50	0.00	0.00	0.00	0.50
....................TTAGGTATACTGAAGGTATA..........................................................	20	2	0.50	0.00	0.00	0.00	0.50
....................TTACGTATAATGAAGGCATACC........................................................	22	2	0.50	0.50	0.00	0.00	0.00
....................TTAAGTATACTGAAGGTATA..........................................................	20	2	0.50	0.00	0.00	0.00	0.50
....................TTACGTATACTGAAGGGATA..........................................................	20	2	0.50	0.00	0.00	0.00	0.50
....................TTACGTATACTGAATGTATA..........................................................	20	2	0.50	0.00	0.00	0.00	0.50
....................TTACGTATACTGAAGGTATACCGGA.....................................................	25	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGTTATA..........................................................	20	2	0.50	0.50	0.00	0.00	0.00
.........................................................AGGATACATTCAGTATACGTTTA..................	23	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAATGTATAC.........................................................	21	2	0.50	0.50	0.00	0.00	0.00
....................GTACGTATACTGAAGGTATACC........................................................	22	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATGC.........................................................	21	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGGAGGTATACC........................................................	22	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGCATA..........................................................	20	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATCCC........................................................	22	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATACTGAAGGTATACCCT......................................................	24	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCG.......................................................	23	2	0.50	0.00	0.50	0.00	0.00
....................TGACGTATACTGAAGGTATACC........................................................	22	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCGG......................................................	24	2	0.50	0.50	0.00	0.00	0.00

droEug1

GTTGCTCGTGCTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACACAATCA

Size

Hit Count

Total Norm

droBia1

GTTGCTCGTGCTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCA

Size

Hit Count

Total Norm

droTak1

GTTGCTCGTGCTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCA

Size

Hit Count

Total Norm

droEle1

GTTGCTCGTGCTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCA

Size

Hit Count

Total Norm

droRho1

GTTGCTCGTGCTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACAGAATCA

Size

Hit Count

Total Norm

droFic1

GTTGCTCGTGCTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTTTACGAGGTCGCACACAATCA

Size

Hit Count

Total Norm

droKik1

GTTGCTTGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAGAGTCA

Size

Hit Count

Total Norm

droAna3

GTTGAAGGTGTTACCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAAAATCA	Size	Hit Count	Total Norm	V105 Male body	V106 Head	V055 Unknown	V039 Embryo
....................TTACGTATACTGAAGGTAT...........................................................	19	2	94.00	76.50	12.50	5.00	0.00
....................TTACGTATACTGAAGGTATACCGT......................................................	24	2	8.00	6.00	2.00	0.00	0.00
....................TTACGTATACTGAAGGTATACC........................................................	22	2	8.00	1.50	3.00	2.50	1.00
....................TTACGTATACTGAAGGTATA..........................................................	20	2	8.00	4.00	4.00	0.00	0.00
..................TGTTACGTATACTGAAGGTAT...........................................................	21	2	7.50	5.50	1.50	0.50	0.00
....................TTACGTATACTGAAGGTATACCGG......................................................	24	2	5.50	0.00	4.00	1.50	0.00
....................TTACGTATACTGAAGGTATACCGGT.....................................................	25	2	4.00	1.50	2.00	0.50	0.00
.............................................................TACATTCAGTATACGTATACGT...............	22	2	3.00	3.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCG.......................................................	23	2	2.00	1.50	0.50	0.00	0.00
.........................................................AGGATACATTCAGTATACGT.....................	20	2	1.50	1.50	0.00	0.00	0.00
....................TTACGTATACTGAATGTAT...........................................................	19	4	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATAC.........................................................	21	2	1.00	1.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATT..........................................................	20	2	1.00	1.00	0.00	0.00	0.00
....................TTACxTATACTGAAGGTAT...........................................................	18	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGCTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTCTACTGAAGGTATACCGGA.....................................................	25	2	0.50	0.00	0.00	0.00	0.50
....................GCACGTATACTGAAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
..................TGTTACGTATACTGAAGGTAG...........................................................	21	2	0.50	0.50	0.00	0.00	0.00
....................TTATGTATACTGAAGGTATA..........................................................	20	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATACTGAAGGCAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
..................TGTTACGTATACTGAAGGGAT...........................................................	21	2	0.50	0.00	0.00	0.50	0.00
....................TTACGTATACTGAAGGTAC...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGGAT...........................................................	19	2	0.50	0.00	0.00	0.50	0.00
....................TTACGTATACTGAAGATAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACGGAAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCGC......................................................	24	2	0.50	0.50	0.00	0.00	0.00
....................TCACGTATACTGAAGGTATACC........................................................	22	2	0.50	0.00	0.00	0.50	0.00
....................TTACGTATGCTGAAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGCATACC........................................................	22	2	0.50	0.00	0.00	0.00	0.50
....................TTACTTATACTGAAGGTAT...........................................................	19	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATACTGAAAGTAT...........................................................	19	4	0.25	0.25	0.00	0.00	0.00
....................TTACGTATACTGTAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
....................TTAGGTATACTGAAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAATGTATAA.........................................................	21	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATACTTAAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACAG.......................................................	23	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGCAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
.................................................AAAGACTCAGGATACATT...............................	18	2	0.50	0.00	0.00	0.50	0.00
....................TTACGTATACCGAAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATAACGT......................................................	24	2	0.50	0.50	0.00	0.00	0.00
....................TTACGGATACTGAAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTAGACTGAAGGTATAC.........................................................	21	2	0.50	0.00	0.00	0.00	0.50
....................TCACGTATACTGAAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
...................GTTACGGATGCTGAAGGT.............................................................	18	2	0.50	0.50	0.00	0.00	0.00

droBip1

GTTGAAAGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAAAATCA

Size

Hit Count

Total Norm

dp4

GTTGCTGGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTCGCACAGGTTC-	Size	Hit Count	Total Norm	V112 Male body	V043 Embryo	V051 Head	M022 Male body
....................TTACGTATACTGAAGGTAT...........................................................	19	2	71.00	49.50	2.50	18.50	0.50
....................TTACGTATACTGAAGGTATA..........................................................	20	2	25.50	5.50	18.50	1.50	0.00
....................TTACGTATACTGAAGGTATAC.........................................................	21	2	15.00	4.50	7.50	3.00	0.00
....................TTACGTATACTGAAGGTATACC........................................................	22	2	11.00	1.00	7.50	2.50	0.00
....................TTACGTATACTGAAGGTATACCGGA.....................................................	25	2	2.00	1.00	0.50	0.50	0.00
....................TTACGTATACTGAAGGTATT..........................................................	20	2	2.00	2.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCGG......................................................	24	2	2.00	0.00	1.50	0.50	0.00
....................TTACGTATACTGAAGGTTTT..........................................................	20	2	1.50	1.00	0.50	0.00	0.00
.......................CGTATACTGAAGGTATACCGGA.....................................................	22	2	1.50	0.00	1.50	0.00	0.00
.........................................................AGGATACATTCAGTATACGTATA..................	23	2	1.00	0.00	1.00	0.00	0.00
.........................................................AGGATACATTCAGTATACGT.....................	20	2	1.00	1.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCGGT.....................................................	25	2	1.00	1.00	0.00	0.00	0.00
....................TTACGTATATTGGAGGTATA..........................................................	20	2	0.50	0.00	0.50	0.00	0.00
....................TTACGGATACTGAAGCTA............................................................	18	2	0.50	0.00	0.00	0.50	0.00
....................TTACGTATACTCAAGGTATTC.........................................................	21	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATACTGAAGGTCTACC........................................................	22	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATACTGAAGGCAT...........................................................	19	2	0.50	0.00	0.00	0.50	0.00
....................TTACGTATACTGAAGTTATAC.........................................................	21	2	0.50	0.00	0.00	0.50	0.00
.............................................................TACATTCAGTATACGTATACGT...............	22	2	0.50	0.50	0.00	0.00	0.00
..................TGTTACGTATACTGAAGGTAT...........................................................	21	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATAATGAAGGTATA..........................................................	20	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATACTGAAGGTATAGCGGT.....................................................	25	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCGT......................................................	24	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATTA.........................................................	21	2	0.50	0.00	0.50	0.00	0.00
....................TGACGTATACTGAAGGTAT...........................................................	19	2	0.50	0.00	0.00	0.50	0.00
....................TTACGTATACTGTAGGTATA..........................................................	20	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATACTGATGGTGTA..........................................................	20	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATACTGAAGGTA............................................................	18	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATACTGAAGGCATACC........................................................	22	2	0.50	0.00	0.50	0.00	0.00
....................TTACGxATACTGAAGGTATA..........................................................	19	2	0.50	0.50	0.00	0.00	0.00
...................CTTACGTATACTGAAGGTAT...........................................................	20	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTTA...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGCAGGTATC..........................................................	20	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATACTGCAGGTAT...........................................................	19	2	0.50	0.00	0.00	0.50	0.00
....................TGACGTATACTGAAGGTATACCGT......................................................	24	2	0.50	0.00	0.00	0.50	0.00
.......................CGTATACTGAAGGTATTCCGGA.....................................................	22	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATGCTGAAGGTATAC.........................................................	21	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATACTGAAGGTTT...........................................................	19	2	0.50	0.00	0.00	0.00	0.50
....................TTACGTATACTGGAGGAATA..........................................................	20	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTACACTGAAGGTAT...........................................................	19	2	0.50	0.00	0.00	0.50	0.00
....................TGACGTATACTGAAGGTATAC.........................................................	21	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATACTGGAGGTATG..........................................................	20	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATACTGAAGGTATACCG.......................................................	23	2	0.50	0.00	0.50	0.00	0.00

droPer1

GTTGCTGGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTCGCACAGGTTC-	Size	Hit Count	Total Norm	V111 Male body	V042 Embryo	V050 Head	V057 Head	M021 Embryo
....................TTACGTATACTGAAGGTAT...........................................................	19	2	259.00	252.50	2.50	3.00	1.00	0.00
....................TTACGTATACTGAAGGTATA..........................................................	20	2	24.50	14.00	9.50	0.50	0.50	0.00
....................TTACGTATACTGAAGGTATT..........................................................	20	2	4.50	4.50	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATAC.........................................................	21	2	3.50	2.00	1.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTTTT..........................................................	20	2	2.50	2.50	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACC........................................................	22	2	2.50	0.50	1.00	0.00	0.00	1.00
....................TTACGTATACTGAAGGGAT...........................................................	19	2	2.00	1.50	0.00	0.50	0.00	0.00
....................TTACGTATACTGAAGGTTTA..........................................................	20	2	1.50	1.50	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGAAT...........................................................	19	2	1.50	1.50	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCGG......................................................	24	2	1.50	1.00	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCGT......................................................	24	2	1.00	1.00	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTTA...........................................................	19	2	1.00	1.00	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTAA...........................................................	19	2	0.50	0.50	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATAA.........................................................	21	2	0.50	0.50	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGCAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00	0.00
.........................................................AGGATACATTCAGTATACGT.....................	20	2	0.50	0.50	0.00	0.00	0.00	0.00
.....................TACGTATACTGAAGGGAT...........................................................	18	4	0.25	0.25	0.00	0.00	0.00	0.00
....................TTACGTATACTGGAGGTATA..........................................................	20	2	0.50	0.50	0.00	0.00	0.00	0.00
....................TTACGTATAGTGAAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAAGTATA..........................................................	20	2	0.50	0.00	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCGGT.....................................................	25	2	0.50	0.50	0.00	0.00	0.00	0.00
....................GTACGTATACTGAAGGTAT...........................................................	19	4	0.25	0.25	0.00	0.00	0.00	0.00
....................TTATGTATACTGAAGGTATACCGG......................................................	24	2	0.50	0.00	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGGATA..........................................................	20	2	0.50	0.00	0.50	0.00	0.00	0.00
....................TTAGGTATACTGAAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATAT.........................................................	21	2	0.50	0.00	0.50	0.00	0.00	0.00
....................TTACGTATACTGACGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00	0.00
....................ATACGTATACTGAAGGTAT...........................................................	19	4	0.25	0.25	0.00	0.00	0.00	0.00
....................TTACGTATACCGTAGGTAT...........................................................	19	2	0.50	0.00	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTTT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00	0.00
....................TTACGTACACTGAAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00	0.00
....................TTACGAATACTGAAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACT........................................................	22	2	0.50	0.50	0.00	0.00	0.00	0.00
....................TTACGCATACTGAAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00	0.00

droWil1

GTTGTTGGTGTTTCATCGTGTTACGTATACTGAAGGTATACCGGATAGCATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAGATTCA	Size	Hit Count	Total Norm	V117 Male body	V118 Embryo	V119 Head	M020 Head
....................TTACGTATACTGAAGGTAT...........................................................	19	2	54.00	33.50	11.50	8.50	0.50
....................TTACGTATACTGAAGGTATA..........................................................	20	2	29.00	3.00	22.00	4.00	0.00
....................TTACGTATACTGAAGGTATAC.........................................................	21	2	23.50	0.50	21.50	1.00	0.50
....................TTACGTATACTGAAGGTATACCGG......................................................	24	2	14.50	0.00	14.50	0.00	0.00
....................TTACGTATACTGAAGGTATACC........................................................	22	2	12.50	0.50	11.00	1.00	0.00
.......................CGTATACTGAAGGTATACCGGA.....................................................	22	2	6.00	0.00	5.50	0.00	0.50
.........................................................AGGATACATTCAGTATACGTA....................	21	2	6.00	0.00	5.00	1.00	0.00
.TTGTTGGTGTTTCATCGTG..............................................................................	19	2	5.00	0.00	5.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCGGA.....................................................	25	2	5.00	0.50	4.50	0.00	0.00
....................TTACGTATACTGAAGGTATACCG.......................................................	23	2	3.50	0.00	3.50	0.00	0.00
.........................................................AGGATACATTCAGTATACGT.....................	20	2	2.50	1.50	1.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCGGT.....................................................	25	2	1.50	0.00	1.50	0.00	0.00
.........................................................AGGATACATTCAGTATACGTATA..................	23	2	1.00	0.00	1.00	0.00	0.00
.........................................................AGGATACATTCAGTATACGTAT...................	22	2	1.00	0.00	1.00	0.00	0.00
....................TCACGTATACTGAAGGTATAC.........................................................	21	2	0.50	0.00	0.50	0.00	0.00
....................TTACTTATACTGAAGGTATA..........................................................	20	2	0.50	0.50	0.00	0.00	0.00
............................................................ATACATTCAGTATACGTATACG................	22	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATACTCAAGGTAT...........................................................	19	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATACTCAAGGTATACCGGA.....................................................	25	2	0.50	0.00	0.50	0.00	0.00
..................TGTTACGTATACTGAAGGTAT...........................................................	21	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCGT......................................................	24	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATGCTGAAGGTAT...........................................................	19	2	0.50	0.00	0.00	0.50	0.00
....................TTACGTATACTGAAGGTA............................................................	18	2	0.50	0.00	0.50	0.00	0.00
....................TTGCGTATACTGAAGGTATA..........................................................	20	2	0.50	0.00	0.00	0.50	0.00
....................TCACGTATACTGAAGGTATACCGG......................................................	24	2	0.50	0.00	0.50	0.00	0.00
....................TTACGxATACTGAAGGTATA..........................................................	19	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATACTGAAxGTATA..........................................................	19	2	0.50	0.00	0.50	0.00	0.00
....................TTAAGTATACTGAAGGTATAC.........................................................	21	2	0.50	0.00	0.50	0.00	0.00
....................TTACGAATACTGAAGGTATACC........................................................	22	2	0.50	0.00	0.50	0.00	0.00
....................TTACGTATACTxAAGGTAT...........................................................	18	3	0.33	0.00	0.33	0.00	0.00
....................TTACGTATACTGAAGGCATA..........................................................	20	2	0.50	0.00	0.00	0.50	0.00
.....................................................ACTCAGGATACATTCAGT...........................	18	2	0.50	0.50	0.00	0.00	0.00

droVir3

GTCGCTGGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAGGCTCT	Size	Hit Count	Total Norm	V116 Male body	V053 Unknown	V047 Embryo	M027 Male body	M028 Head
....................TTACGTATACTGAAGGTAT...........................................................	19	2	41.00	39.00	0.50	0.50	0.50	0.50
....................TTACGTATACTGAAGGTATA..........................................................	20	2	2.50	2.00	0.00	0.50	0.00	0.00
....................TTACGTATACTGAAGGTATACC........................................................	22	2	1.50	0.50	0.00	0.50	0.50	0.00
....................TTACGTATACTGAAGGTATAC.........................................................	21	2	1.00	1.00	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGGAT...........................................................	19	2	1.00	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCGT......................................................	24	2	1.00	1.00	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCG.......................................................	23	2	1.00	0.50	0.00	0.00	0.50	0.00
....................TTACGTATACTGAAGGTAA...........................................................	19	2	0.50	0.50	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCCG......................................................	24	2	0.50	0.00	0.00	0.50	0.00	0.00
....................TTACGTATACTGAAGGTATACCGGA.....................................................	25	2	0.50	0.50	0.00	0.00	0.00	0.00
.........................................................AGGATACATTCAGTATACGTAA...................	22	2	0.50	0.50	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATAA.........................................................	21	2	0.50	0.50	0.00	0.00	0.00	0.00
.......GTTTTTCCTCGTGTTAAGT........................................................................	19	2	0.50	0.00	0.00	0.50	0.00	0.00
.........................................................AGGATACATTCAGTATACGT.....................	20	2	0.50	0.50	0.00	0.00	0.00	0.00
GTCGCTGGTGTTTCCTCGTG..............................................................................	20	2	0.50	0.00	0.00	0.50	0.00	0.00
....................TTACGCATACTGAAGTTATACC........................................................	22	2	0.50	0.00	0.00	0.50	0.00	0.00
....................CTACGTATACTGAAGGTAT...........................................................	19	4	0.25	0.25	0.00	0.00	0.00	0.00
..........................................................GGATACATTCAGTATACGTATA..................	22	2	0.50	0.00	0.00	0.50	0.00	0.00
....................TTACGTATACTGAAGGTATACCGGTC....................................................	26	2	0.50	0.00	0.00	0.00	0.50	0.00
....................TTACGTATACAGAAGGTATACCGG......................................................	24	2	0.50	0.50	0.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATC..........................................................	20	4	0.25	0.25	0.00	0.00	0.00	0.00
...................CTTACGTATACTGAAGGTATACCGGT.....................................................	26	2	0.50	0.00	0.00	0.50	0.00	0.00
.........................................................AGGATACATTCAGTATACG......................	19	2	0.50	0.00	0.50	0.00	0.00	0.00

droMoj3

GTTGCTGGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAGGCTCA	Size	Hit Count	Total Norm	V110 Male body	V056 Unknown	V049 Head	V041 Embryo
....................TTACGTATACTGAAGGTAT...........................................................	19	2	220.50	213.50	4.00	3.00	0.00
....................TTACGTATACTGAAGGTATA..........................................................	20	2	12.50	11.00	0.00	0.00	1.50
....................TTACGTATACTGAAGGTATACC........................................................	22	2	10.50	4.50	0.50	0.00	5.50
....................TTACGTATACTGAAGGTATAC.........................................................	21	2	6.50	4.50	0.00	0.00	2.00
....................TTACGTATACTGAAGGTATACCGG......................................................	24	2	5.50	2.00	0.50	0.00	3.00
....................TTACGTATACTGAAGGTATACCGT......................................................	24	2	5.00	5.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCGGT.....................................................	25	2	3.50	3.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCG.......................................................	23	2	2.50	1.50	0.50	0.00	0.50
....................TTACGTATACTGAAGGTATAA.........................................................	21	2	1.50	1.00	0.50	0.00	0.00
....................TTACGTATACTGAATGTAT...........................................................	19	4	0.75	0.75	0.00	0.00	0.00
....................TTACGTATACTGAAGGTATACCGGA.....................................................	25	2	1.50	1.00	0.00	0.00	0.50
....................TTACGTATACTGAAGGCAT...........................................................	19	2	1.00	1.00	0.00	0.00	0.00
.........................................................AGGATACATTCAGTATACGT.....................	20	2	1.00	1.00	0.00	0.00	0.00
....................TTACGTATACTGAAGGGAT...........................................................	19	2	1.00	1.00	0.00	0.00	0.00
.........................................................AGGATACATTCAGTATACGTA....................	21	2	1.00	0.00	0.00	0.00	1.00
...........................................GATAGGATACACTCAGGATACATTCAGT...........................	28	2	0.50	0.50	0.00	0.00	0.00
..........................ATACTGAAGGTATACCGGT.....................................................	19	2	0.50	0.50	0.00	0.00	0.00
.TTGCTGGTGTTTCATAGTG..............................................................................	19	2	0.50	0.00	0.00	0.00	0.50
....................TTACGTATACTGAAGGTTC...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTGTACTGAAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
....................TTACGGATACGGAAGGTAT...........................................................	19	2	0.50	0.00	0.50	0.00	0.00
....................TTTCGTATACTGAAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAAGGTAC...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAGGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
..................TGTTACGTATACTGAAGGTAT...........................................................	21	2	0.50	0.50	0.00	0.00	0.00
.....................................ATACCGGATAGGATACACTCAGGAT....................................	25	2	0.50	0.50	0.00	0.00	0.00
......................................................CTCAGGATACATTCAGTA..........................	18	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGAGGGTATA..........................................................	20	2	0.50	0.00	0.00	0.00	0.50
....................TTACTTATACTGAAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
..................TGTTACGTACACTGAAGGTAT...........................................................	21	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACTGTAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
.......................CGTATACTGAAGGTATACCGGA.....................................................	22	2	0.50	0.00	0.00	0.00	0.50
....................TTACGTATACTGAAGGGATACCG.......................................................	23	2	0.50	0.00	0.00	0.00	0.50
....................TTACGTATACTGAAGGGATACCGT......................................................	24	2	0.50	0.50	0.00	0.00	0.00
......................................................CTCAGGATACATTCAGTATACGTATA..................	26	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTxTACTGAAGGTAT...........................................................	18	2	0.50	0.50	0.00	0.00	0.00
....................TTACGTATACCGAAGGTAT...........................................................	19	2	0.50	0.50	0.00	0.00	0.00
....................TTACGCATACTGAAGGGATACC........................................................	22	2	0.50	0.00	0.00	0.00	0.50
....................TTACGTATACTGAACGCATACC........................................................	22	2	0.50	0.00	0.00	0.00	0.50
....................TTACGTTTACTGAAGGTATACC........................................................	22	2	0.50	0.00	0.00	0.00	0.50
............................................ATAGGATACACTCAGGAT....................................	18	2	0.50	0.00	0.00	0.50	0.00

droGri2

GTTGCTGGTGTTTCCTCGTGTTACGTATACTGAAGGTATACCGGATAGGATACACTCAGGATACATTCAGTATACGTATACGAAGTTGCACAG-----	Size	Hit Count	Total Norm	V109 Male body
....................TTACGTATACTGAAGGTAT...........................................................	19	2	9.50	9.50
....................TTACGTATACTGAAGGTTTA..........................................................	20	2	0.50	0.50
....................TTACGTATACTGAAGGTCT...........................................................	19	2	0.50	0.50
....................TTACGTATACTGAAGGTATACCGT......................................................	24	2	0.50	0.50
....................TTACGTATACTGAAGGTGT...........................................................	19	2	0.50	0.50
....................TTACGTATACTGAAGGTATCC.........................................................	21	4	0.25	0.25
....................TTACGTATACTGAAGGTATA..........................................................	20	2	0.50	0.50
....................TTCCGTATACTGAAGGTAT...........................................................	19	2	0.50	0.50

Generated: 04/20/2012 at 05:31:02 EDT

dme-mir-iab-8

chr3R:12681997-12682064 -

Known_mirbase

intergenic

20-way Fast Statistical Aligner (FSA) re-alignment

Visually pleasing best hits from LASTZ and MAF

Legend

Re-alignment after removal of non-confident orthologs

20-way Secondary Structure

Small RNA-seq read pileup