| TCGATA---TCCCAG-AAAAAT----GA-TGAAA-------------------------------------------------------------------------------------------TTTCGCGTTCGCATTCACACTAGCTGAGTAATGGGTATC------------TGATTGTCGGGGAA-CTCGACTAT------------AG-CATTCTCTTTTGATTTTATTTA-------TATTTTTG------C------T-------TAA-------------------------------------------------------------------------------------------ATATT-TTG---------------------------------------T----ATACATAAA---------------------------------------------ATGTTTTAT-----AATTATGTTTATT---CATT----------------------TTTAT---ACTCCTTAAGGTTG----------------------------------- | Size | Hit Count | Total Norm | Total | M023 Head | M024 Male-body | M025 Embryo | M053 Female-body | O001 Testis | O002 Head | SRR553485 Ovary | SRR553486 Ovary | SRR553487 Ovary | SRR553488 Ovary | SRR618934 Ovary | V044 Embryo | SRR902008 Ovary | SRR902009 Testis | SRR1275487 Male larvae | SRR1275485 Male prepupae | SRR1275483 Male prepupae |
| ..................................................................................................................................................................TC------------TGATTGTCGGGGAA-CTCGACTAT------------....................................................................................................................................................................................................................................................................................................................................................................................... | 25 | 3 | 2.67 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................TGAGTAATGGGTATC------------TGATTGTCGG................................................................................................................................................................................................................................................................................................................................................................................................................. | 25 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................................................................TGTCGGGGAA-CTCGACTA.................................................................................................................................................................................................................................................................................................................................................................................................... | 18 | 10 | 0.70 | 7 | 0 | 0 | 0 | 0 | 6 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................GGAA-CTCGACTAT------------AG-CATTCTCTTTT......................................................................................................................................................................................................................................................................................................................................................................... | 26 | 7 | 0.57 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................GTATC------------TGATTGTCGGGGAA-CTCGACTAT------------....................................................................................................................................................................................................................................................................................................................................................................................... | 28 | 2 | 0.50 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................GGGTATC------------TGATTGTCGGGGAA-C........................................................................................................................................................................................................................................................................................................................................................................................................... | 22 | 4 | 0.50 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................AA-CTCGACTAT------------AG-CATTCTCTTTTGATT..................................................................................................................................................................................................................................................................................................................................................................... | 28 | 2 | 0.50 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..............................................................................................................................................CACTAGCTGAGTAATGGGTATC------------TG......................................................................................................................................................................................................................................................................................................................................................................................................................... | 24 | 13 | 0.46 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................TTCACACTAGCTGAGTA................................................................................................................................................................................................................................................................................................................................................................................................................................................ | 17 | 20 | 0.35 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
| .................................................................................................................................................................ATC------------TGATTGTCGGGGAA-CTCGACTAT------------....................................................................................................................................................................................................................................................................................................................................................................................... | 26 | 3 | 0.33 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .........TCCCAG-AAAAAT----GA-TGAAA-------------------------------------------------------------------------------------------TTTCGCGTT..................................................................................................................................................................................................................................................................................................................................................................................................................................................................... | 28 | 18 | 0.33 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .................................................................................................................................................................ATC------------TGATTGTCGGGGAA-CT.......................................................................................................................................................................................................................................................................................................................................................................................................... | 19 | 6 | 0.33 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ................................................................................................................................................................TATC------------TGATTGTCGGGGAA-CTCGAC...................................................................................................................................................................................................................................................................................................................................................................................................... | 24 | 3 | 0.33 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ................................................................................................................................................................TATC------------TGATTGTCGGGGAA-CTCGACT..................................................................................................................................................................................................................................................................................................................................................................................................... | 25 | 3 | 0.33 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................GGAA-CTCGACTAT------------AG-CATTCTCTT........................................................................................................................................................................................................................................................................................................................................................................... | 24 | 9 | 0.33 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 |
| ..............................................................................................................................................................GGTATC------------TGATTGTCGGGGAA-CTC......................................................................................................................................................................................................................................................................................................................................................................................................... | 23 | 4 | 0.25 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................GGAA-CTCGACTAT------------AG-CATTC............................................................................................................................................................................................................................................................................................................................................................................... | 20 | 20 | 0.25 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 0 |
| .........TCCCAG-AAAAAT----GA-TGAAA-------------------------------------------------------------------------------------------TTTCGCGT...................................................................................................................................................................................................................................................................................................................................................................................................................................................................... | 27 | 19 | 0.21 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ................................................................................................................................................................TATC------------TGATTGTCGGGGAA-CTCGA....................................................................................................................................................................................................................................................................................................................................................................................................... | 23 | 5 | 0.20 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................TTGTCGGGGAA-CTCGACTAT------------AG-C................................................................................................................................................................................................................................................................................................................................................................................... | 23 | 6 | 0.17 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .........................................................................................................................................ATTCACACTAGCTGAGTAATGGG........................................................................................................................................................................................................................................................................................................................................................................................................................................... | 23 | 6 | 0.17 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................TTCACACTAGCTGAGTAATGGGTATC------------........................................................................................................................................................................................................................................................................................................................................................................................................................... | 26 | 6 | 0.17 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................ACACTAGCTGAGTAATGGGTA......................................................................................................................................................................................................................................................................................................................................................................................................................................... | 21 | 6 | 0.17 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................TCGGGGAA-CTCGACTAT------------AG-CATTCTC............................................................................................................................................................................................................................................................................................................................................................................. | 26 | 20 | 0.15 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 |
| .CGATA---TCCCAG-AAAAAT----GA-TGAAA-------------------------------------------------------------------------------------------.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................. | 24 | 20 | 0.15 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
| .CGATA---TCCCAG-AAAAAT----GA-TGAAA-------------------------------------------------------------------------------------------T............................................................................................................................................................................................................................................................................................................................................................................................................................................................................. | 25 | 20 | 0.15 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................CACACTAGCTGAGTAATGGGT.......................................................................................................................................................................................................................................................................................................................................................................................................................................... | 21 | 7 | 0.14 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .........................................................................................................................................ATTCACACTAGCTGAGTAATGGGA.......................................................................................................................................................................................................................................................................................................................................................................................................................................... | 24 | 8 | 0.13 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .................................................................................................................................................................................GATTGTCGGGGAA-CTC......................................................................................................................................................................................................................................................................................................................................................................................................... | 16 | 8 | 0.13 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................GGAA-CTCGACTAT------------AG-CATTCTCTTT.......................................................................................................................................................................................................................................................................................................................................................................... | 25 | 8 | 0.13 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................GAA-CTCGACTAT------------AG-CATTCTCTTTTG........................................................................................................................................................................................................................................................................................................................................................................ | 26 | 9 | 0.11 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................TTCGCATTCACACTAGCTGAGT................................................................................................................................................................................................................................................................................................................................................................................................................................................. | 22 | 20 | 0.10 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................CGCATTCACACTAGCTGA................................................................................................................................................................................................................................................................................................................................................................................................................................................... | 18 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................AA-CTCGACTAT------------AG-CATTCTCTTTTG........................................................................................................................................................................................................................................................................................................................................................................ | 25 | 10 | 0.10 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................GTCGGGGAA-CTCGACTAT------------AG-CATTCTC............................................................................................................................................................................................................................................................................................................................................................................. | 27 | 20 | 0.10 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................................................A-CTCGACTAT------------AG-CATT................................................................................................................................................................................................................................................................................................................................................................................ | 16 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| TCGATA---TCCCAG-AAAAAT----GA-TGAA.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... | 24 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................GGAA-CTCGACTAT------------AG-CATTCTCTTA.......................................................................................................................................................................................................................................................................................................................................................................... | 25 | 11 | 0.09 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| .................................................................................................................................................TAGCTGAGTAATGGGTATC------------TGAT....................................................................................................................................................................................................................................................................................................................................................................................................................... | 23 | 13 | 0.08 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .................................................................................................................................................TAGCTGAGTAATGGGTATC------------TGA........................................................................................................................................................................................................................................................................................................................................................................................................................ | 22 | 15 | 0.07 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..............................................................................................................................................CACTAGCTGAGTAATGGGTATC------------TGT........................................................................................................................................................................................................................................................................................................................................................................................................................ | 25 | 15 | 0.07 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ....TA---TCCCAG-AAAAAT----GA-TGAAA-------------------------------------------------------------------------------------------TTTCGCGTT..................................................................................................................................................................................................................................................................................................................................................................................................................................................................... | 30 | 16 | 0.06 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..............................................................................................................................................CACTAGCTGAGTAATGGGTAT........................................................................................................................................................................................................................................................................................................................................................................................................................................ | 21 | 16 | 0.06 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..............................................................................................................................................CACTAGCTGAGTAATGG............................................................................................................................................................................................................................................................................................................................................................................................................................................ | 17 | 18 | 0.06 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....A---TCCCAG-AAAAAT----GA-TGAAA-------------------------------------------------------------------------------------------TTTCGCGT...................................................................................................................................................................................................................................................................................................................................................................................................................................................................... | 28 | 18 | 0.06 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................TCGGGGAA-CTCGACTAT------------AG-CATT................................................................................................................................................................................................................................................................................................................................................................................ | 23 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................CGGGGAA-CTCGACTAT------------AG-C................................................................................................................................................................................................................................................................................................................................................................................... | 19 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..................................................................................................................................................AGCTGAGTAATGGGTA......................................................................................................................................................................................................................................................................................................................................................................................................................................... | 16 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................CGGGGAA-CTCGACTAT------------AG-CATTCTC............................................................................................................................................................................................................................................................................................................................................................................. | 25 | 20 | 0.05 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..GATA---TCCCAG-AAAAAT----GA-T............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. | 19 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........CCCAG-AAAAAT----GA-TGAAA-------------------------------------------------------------------------------------------TTTCGCGTT..................................................................................................................................................................................................................................................................................................................................................................................................................................................................... | 27 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................A-TGAAA-------------------------------------------------------------------------------------------TTTCGCGTTCGCA................................................................................................................................................................................................................................................................................................................................................................................................................................................................. | 19 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ................................................................................................................................CGCGTTCGCATTCACACTAGCTGAG.................................................................................................................................................................................................................................................................................................................................................................................................................................................. | 25 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................AAT----GA-TGAAA-------------------------------------------------------------------------------------------TTTCGCGTT..................................................................................................................................................................................................................................................................................................................................................................................................................................................................... | 19 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .........................................................................................................................................................................................GGGAA-CTCGACTAT------------AG-CATTCTCT............................................................................................................................................................................................................................................................................................................................................................................ | 24 | 20 | 0.05 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..................................................................................................................................................AGCTGAGTAATGGGTATC------------TGA........................................................................................................................................................................................................................................................................................................................................................................................................................ | 21 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .................................A-------------------------------------------------------------------------------------------TTTCGCGTTCGCATTCACACTAGC...................................................................................................................................................................................................................................................................................................................................................................................................................................................... | 25 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................ACTAGCTGAGTAATGGG........................................................................................................................................................................................................................................................................................................................................................................................................................................... | 17 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........CCCAG-AAAAAT----GA-TGAAA-------------------------------------------------------------------------------------------TT............................................................................................................................................................................................................................................................................................................................................................................................................................................................................ | 20 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |