| AACAATTAGATACAATCGGAATTATACCGGTTATTATTTCGGGTTTAATCTATTAAATCGA------------ATAATTTCATATGCC-------------------------------TAGAGGCA-------------------------------GCG---------------------A-------------------AGTC-----------------------------------------------------------T-TATAT-------------------------GA-CAAA---T---------------GATACTTTCTGG----TTTTTAT-------ACCCGTTACTCG--TAGAGTGAAAGGGTATACTAGATTCGTTCAA-------AAGTATGTAACAGGCAGAAGG-----------ACTATACAAAGTATATATA----------TTCTTTATC-A---------------GGAT-----C----TGGTCAA-AAAGA--A-AA----GTTTT-------- | Size | Hit Count | Total Norm | Total | M023 Head | M024 Male-body | M025 Embryo | M053 Female-body | O001 Testis | O002 Head | SRR553485 Ovary | SRR553486 Ovary | SRR553487 Ovary | SRR553488 Ovary | SRR618934 Ovary | V044 Embryo | SRR902008 Ovary | SRR902009 Testis | SRR1275487 Male larvae | SRR1275485 Male prepupae | SRR1275483 Male prepupae |
| ............................................................................................................................................................................................................................................................................................................................................................................................................AA-------AAGTATGTAACAGGCAGAAGG-----------ACT...................................................................................................... | 26 | 1 | 6.00 | 6 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................................................................AA-------AAGTATGTAACAGGCAGAAGG-----------A........................................................................................................ | 24 | 20 | 3.65 | 73 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 73 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................................................................................................TTCGTTCAA-------AAGTATGTAACAGGCAGA....................................................................................................................... | 27 | 1 | 2.00 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................................................TCG--TAGAGTGAAAGGGTATACTAGA......................................................................................................................................................... | 25 | 6 | 2.00 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................................................................................................TTCGTTCAA-------AAGTATGTAACAGGCA......................................................................................................................... | 25 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................................................................AA-------AAGTATGTAACAGGCAGAAGG-----------ACTT..................................................................................................... | 27 | 2 | 1.00 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................................................................AA-------AAGTATGTAACAGGCAGAAGG-----------ACTA..................................................................................................... | 27 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| ........................................................................................................................................................................................................................................................................................................................................................................................................................TATGTAACAGGCAGAAGG-----------ACTATA................................................................................................... | 24 | 2 | 1.00 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................................................................................................TTCGTTCAA-------AAGTATGTAACAGGC.......................................................................................................................... | 24 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................................................................................................................................................ACTATACAAAGTATATATA----------............................................................................ | 19 | 4 | 0.75 | 3 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................................................................................................................TAGAGTGAAAGGGTATACTAGATT....................................................................................................................................................... | 24 | 6 | 0.67 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................................................................................................................................................................................................................................TTTTAT-------ACCCGTTACTCG--TAGAGTGA....................................................................................................................................................................... | 26 | 2 | 0.50 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ........................................................................................................................................................................................................................................................................................................................................................................................................................TATGTAACAGGCAGAAGG-----------ACTATAC.................................................................................................. | 25 | 2 | 0.50 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................................................................................TAACAGGCAGAAGG-----------ACTATACA................................................................................................. | 22 | 2 | 0.50 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................................................................................................TGTAACAGGCAGAAGG-----------ACT...................................................................................................... | 19 | 2 | 0.50 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ........................................................................................................................................................................................................................................................................................................................................................................................................................TATGTAACAGGCAGAAGG-----------ACT...................................................................................................... | 21 | 2 | 0.50 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .........................................................................................................................................................................................................................................................................................................................................................................................................TTCAA-------AAGTATGTAACAGGCAGAAG..................................................................................................................... | 25 | 2 | 0.50 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ........................................................................................................................................................................................................................................................................................................................................................................................................................TATGTAACAGGCAGAAGG-----------ACTATACA................................................................................................. | 26 | 2 | 0.50 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................................................................AA-------AAGTATGTAACAGGCAGAAGG-----------AC....................................................................................................... | 25 | 4 | 0.50 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................................................TCG--TAGAGTGAAAGGGTATACTAG.......................................................................................................................................................... | 24 | 7 | 0.43 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................................................................................................................TAGAGTGAAAGGGTATACTAGAT........................................................................................................................................................ | 23 | 6 | 0.33 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................................................................AA-------AAGTATGTAACAGGCAGAAGG-----------ACTTC.................................................................................................... | 28 | 3 | 0.33 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................................................................................................................................................................................................................................................CGTTACTCG--TAGAGTGAAAGGGTA................................................................................................................................................................ | 24 | 4 | 0.25 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ........................................................................................................................................................................................................................................................................................................................................................................................................................TATGTAACAGGCAGAAGG-----------A........................................................................................................ | 19 | 20 | 0.20 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................................................................GTTACTCG--TAGAGTGAAAGGGTATA.............................................................................................................................................................. | 25 | 5 | 0.20 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................................................TCG--TAGAGTGAAAGGGTATACTAGATTC...................................................................................................................................................... | 28 | 5 | 0.20 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................................................TCG--TAGAGTGAAAGGGT................................................................................................................................................................. | 17 | 10 | 0.20 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| .........................................................................................................................................................................................................................................................................................................................................................................CTCG--TAGAGTGAAAGGGTATACTAGA......................................................................................................................................................... | 26 | 6 | 0.17 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................................................TCG--TAGAGTGAAAGGGTATACTAGAT........................................................................................................................................................ | 26 | 6 | 0.17 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................................................................................................................................................................................................................................TTTTAT-------ACCCGTTACTCG--TAGAGT......................................................................................................................................................................... | 24 | 20 | 0.15 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................................................................................................................................................................................................TACTCG--TAGAGTGAAAGGGTATA.............................................................................................................................................................. | 23 | 7 | 0.14 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................................................TTACTCG--TAGAGTGAAAGGGTATA.............................................................................................................................................................. | 24 | 7 | 0.14 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................................................TCG--TAGAGTGAAAGGGTATACTA........................................................................................................................................................... | 23 | 7 | 0.14 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................................................................................................................................................................................................TACTCG--TAGAGTGAAAGGGTATACT............................................................................................................................................................ | 25 | 7 | 0.14 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................................................TCG--TAGAGTGAAAGGGTATAC............................................................................................................................................................. | 21 | 7 | 0.14 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................................................................GTGAAAGGGTATACTAGATTC...................................................................................................................................................... | 21 | 8 | 0.13 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................................................TCG--TAGAGTGAAAGGGTA................................................................................................................................................................ | 18 | 8 | 0.13 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................................TTTTTAT-------ACCCGTTACTCG--TAGAGT......................................................................................................................................................................... | 25 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................................................TCG--TAGAGTGAAAGGG.................................................................................................................................................................. | 16 | 10 | 0.10 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................................................................AA-------AAGTATGTAACAGGC.......................................................................................................................... | 17 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................................................................................................................................................................................................TAGAGTGAAAGGGTATA.............................................................................................................................................................. | 17 | 10 | 0.10 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................................................TCG--TAGAGTGAAAGG................................................................................................................................................................... | 15 | 10 | 0.10 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................................................................................................................................................................................................................................................................TGAAAGGGTATACTAGATTCG..................................................................................................................................................... | 21 | 10 | 0.10 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................................G--TAGAGTGAAAGGGTATACTAGATTA...................................................................................................................................................... | 26 | 12 | 0.08 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................................................TCG--TAGAGTGAAAGGGTATACTAA.......................................................................................................................................................... | 24 | 14 | 0.07 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................................................TCG--TAGAGTGAAAGGGTATACTATT......................................................................................................................................................... | 25 | 16 | 0.06 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .....................................................................................................................................................................................................................................................................................................................................................................................GAAAGGGTATACTAGA......................................................................................................................................................... | 16 | 17 | 0.06 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................................................................................................................................................CAA-------AAGTATGTAACAGGCAGAAGG-----------AA....................................................................................................... | 26 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................................................................................................................................................................................TAT-------ACCCGTTACTCG--TAGAGT......................................................................................................................................................................... | 21 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................................................................................................................................................................................................................................TTTTAT-------ACCCGTTACTCG--T.............................................................................................................................................................................. | 19 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..........................................................................................................................................................................................................................................................................................................................................................................................................................TGTAACAGGCAGAAGG-----------A........................................................................................................ | 17 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...................................................................................................................................................................................................................................................................................................................................................TTTTTAT-------ACCCGTTACTCG--............................................................................................................................................................................... | 19 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |