ID:

dwi_815

Coordinate:

scf2_1100000004513:501273-501423 +

Confidence:

Candidate

Type:

Unknown

[View on UCSC Genome Browser {Cornell Mirror}]

Legend:

mature

star

mismatch in alignment

mismatch in read

Predicted structure

-14.5	-14.2	-14.2

Flybase annnotation

CDS [Dwil\GK21686-cds]; exon [dwil_GLEANR_4786:2]; intron [Dwil\GK21686-in]

No Repeatable elements found

Sense Strand Reads

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------################################################## CTCAAAGTAGACAAAAACAAATATGCCCTATACTAATTGTAAGTTATACAAATACAAAAGAAAACTTGAGTGCCCTAAGAACGACCTGATAAGATAACTTTATACAAAATACATACATACATACCTACATATTTATAATCCATGGTGGGCATCTAAAATAGGCCACTAATCGAAAATTTGTCATAATGTTTACTATTTTAGCAACTTCTACAAGCATCAGTTCCAGAGGCAAAGCCCCATCCAGATGAGCG ************************************************...................((((((........(((.(((.(((((.................................)))))...)))...)))))))))(((((((((...((.....................))...)))))))))************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V118 embryo	M020 head	M045 female body
..........................................................................................................................................................................................................................AGTTCCAGAGGCAAAGCCCC.............	20	0	1	1.00	1	1	0	0
..................................................................................................................................................................CCACTAATCGAAAATTTGTC.....................................................................	20	0	1	1.00	1	1	0	0
...................................................................................................................................................................................................................................GGCAAAGCCCCATCCAGATGAGC.	23	0	1	1.00	1	1	0	0
.........................................AGTTATACAGATGCAAAAGAC.............................................................................................................................................................................................	21	3	17	0.35	6	0	6	0
.................................................................................................................................................................................................................................GAGGTAAAGGCCCATCAAGA......	20	3	6	0.17	1	0	0	1
............................................................................CAGCACAACCTGATAAGAT............................................................................................................................................................	19	3	20	0.05	1	0	0	1
..........................................GTTATACAGATGCAAAAGAC.............................................................................................................................................................................................	20	3	20	0.05	1	0	1	0

Anti-sense strand reads

GAGTTTCATCTGTTTTTGTTTATACGGGATATGATTAACATTCAATATGTTTATGTTTTCTTTTGAACTCACGGGATTCTTGCTGGACTATTCTATTGAAATATGTTTTATGTATGTATGTATGGATGTATAAATATTAGGTACCACCCGTAGATTTTATCCGGTGATTAGCTTTTAAACAGTATTACAAATGATAAAATCGTTGAAGATGTTCGTAGTCAAGGTCTCCGTTTCGGGGTAGGTCTACTCGC ************************************************...................((((((........(((.(((.(((((.................................)))))...)))...)))))))))(((((((((...((.....................))...)))))))))************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M045 female body
.......................................................................................................................................................................................................................................GTCGGGCTTGGTCTACTCGC	20	3	4	0.25	1	1
............................................................................................................................................................................................................................ATGGTCTCCGTTTCCGGGTG...........	20	3	14	0.07	1	1
...............................................................................................................GTATGTATGTATGGATGTAT........................................................................................................................	20	0	16	0.06	1	1

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droWil2	scf2_1100000004513:501223-501473 +	dwi_815	CTCAAAGTAGACAAAAACAAATATGCCCTATACTAATTGTAAGTTATACAAATACAAAAGAAAACT-----TGAGTGCCCTAAGAACGACCTGATAAGATAACTTTATACAAAATACATACATACATACCTACATATTTATAATCCATGGTGGGCATCTAAAATAGGCCACTAATCGAAAATTTGTCATAATGTTTACTAT----------------------------------------TTTAGCAACTTCTACAAGCATCAGTTCCAGAGGCAAAGCCCCATCCAGATGAGCG
dp5	3:11076026-11076075 +		T------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTCAGCAATACCAGCAA------GCCCCAGAGACCCGTGCAGAGCCAGATGAGAG
droPer2	scaffold_4:6394984-6395033 +		T------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTCAGCAATACCAGCAA------GCCCCAGAGACCCGTGCAGAGCCAGATGAGAG
droKik1	scf7180000302476:1399680-1399861 -		A---------------------------------------------CACAAACACAAAAGAAAACTACTCTTGGG------------GCCCTGATAAGATCA------------------------------AAAGCGTGTCATCCATGGTTAGC-------------------TGGAGGCTCTGCCAGAACT--AAAGCCTTTAGTAACGCACTTCTACGTATTTTTTCTCTTTCCAAATCGCAGCAGTACGTCCCA------GCTCCGGAGGCCCGGCCAGTGCCCGATGAGCG
droTak1	scf7180000415762:498935-498967 +		-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------A------GCTCCGGAGGCGCGGCCAGTGCCCGATGAGCG

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droWil2

CTCAAAGTAGACAAAAACAAATATGCCCTATACTAATTGTAAGTTATACAAATACAAAAGAAAACT-----TGAGTGCCCTAAGAACGACCTGATAAGATAACTTTATACAAAATACATACATACATACCTACATATTTATAATCCATGGTGGGCATCTAAAATAGGCCACTAATCGAAAATTTGTCATAATGTTTACTAT----------------------------------------TTTAGCAACTTCTACAAGCATCAGTTCCAGAGGCAAAGCCCCATCCAGATGAGCG	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
.......................................................................................................................................................................................................................................................................AGTTCCAGAGGCAAAGCCCC.............	20	1	1.00	1	0	0	0	1	0
.......................................................................................................................................................................CCACTAATCGAAAATTTGTC.............................................................................................................	20	1	1.00	1	0	0	0	1	0
................................................................................................................................................................................................................................................................................GGCAAAGCCCCATCCAGATGAGC.	23	1	1.00	1	0	0	0	1	0

dp5

T------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTCAGCAATACCAGCAA------GCCCCAGAGACCCGTGCAGAGCCAGATGAGAG	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
....................................................................................................................................................................................................................................................................................CGTGCAGAGCCAGATGAGAG	20	1	1.00	1	0	0	0	0	0	0	1	0	0	0

droPer2

T------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTCAGCAATACCAGCAA------GCCCCAGAGACCCGTGCAGAGCCAGATGAGAG	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
.............................................................................................................................................................................................................................................................................AGAGACCCGTGCAGAGCCAG.......	20	1	1.00	1	0	0	0	0	0	1

droKik1

A---------------------------------------------CACAAACACAAAAGAAAACTACTCTTGGG------------GCCCTGATAAGATCA------------------------------AAAGCGTGTCATCCATGGTTAGC-------------------TGGAGGCTCTGCCAGAACT--AAAGCCTTTAGTAACGCACTTCTACGTATTTTTTCTCTTTCCAAATCGCAGCAGTACGTCCCA------GCTCCGGAGGCCCGGCCAGTGCCCGATGAGCG	Size	Hit Count	Total Norm	Total
No Reads

droTak1

-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------A------GCTCCGGAGGCGCGGCCAGTGCCCGATGAGCG	Size	Hit Count	Total Norm	Total
No Reads

Generated: 05/17/2015 at 11:55 PM