dwi_72

CTAAGTGATCGTCGCAGGATCATAAAAATCATATCTTATATGCAAAGTAGAGTTCAAGTAAGGGTAAGGAGCGTTATAAATCGAAATCTTCCGACTTTTACTTGATCTTATCTCGGCGTATAAAAAACTCATAAAAAAAATACAAATCAACATTTTTCTA *********************************((((((((..((..(((.((((((((((((..((((.(((.........))).)))).)))))))))))).)))..))..)))))))).**********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M020 head	M045 female body	V117 male body	V118 embryo	V119 head
........................................................................................TTCCGACTTTTACTTGATCTT...................................................	21	0	3	90.00	270	165	62	24	11	8
........................................................................................TTCCGACTTTTACTTGATCTTA..................................................	22	0	3	30.00	90	5	35	15	35	0
........................................................................................TTCCGACTTTTACTTGATCT....................................................	20	0	3	21.00	63	55	3	4	0	1
..................................................AGTTCAAGTAAGGGTAAGGAGCG.......................................................................................	23	0	4	6.00	24	0	3	15	6	0
........................................................................................TTCCGACTTTTACTTGATCTTAA.................................................	23	1	3	4.67	14	2	10	0	1	1
...............................................TAGAGTTCAAGTAAGGGTAAGGA..........................................................................................	23	0	4	3.50	14	0	0	5	9	0
..................................................AGTTCAAGTAAGGGTAAGGAGC........................................................................................	22	0	4	3.00	12	0	2	9	0	1
...............................................TAGAGTTCAAGTAAGGGTAAG............................................................................................	21	0	4	1.75	7	0	0	7	0	0
...............................................TAGAGTTCAAGTAAGGGTAAGG...........................................................................................	22	0	4	1.75	7	0	0	6	1	0
.........................................................................................TCCGACTTTTACTTGATCTT...................................................	20	0	3	1.67	5	0	5	0	0	0
...............................................TAGAGTTCAAGTAAGGGTAA.............................................................................................	20	0	4	1.00	4	0	0	3	1	0
......................................................................................GCTTCCGACTTTTACTTGATCTT...................................................	23	1	1	1.00	1	0	1	0	0	0
........................................................................................TTCCGACTTTTAATTGATCTT...................................................	21	1	3	1.00	3	2	0	0	1	0
.......................................................................................CTTCCGACTTTTACTTGATCTT...................................................	22	0	1	1.00	1	0	0	0	1	0
........................................................................................TTCCGACTTTTACTTGATCTTC..................................................	22	1	3	0.67	2	1	1	0	0	0
..................................................AGTTCAAGTAAGGGTAAGGA..........................................................................................	20	0	4	0.50	2	0	0	2	0	0
..................................................AGTTCAAGTAAGGGTAAGGAG.........................................................................................	21	0	4	0.50	2	0	1	1	0	0
........................................................................................TGCCGACTTTTACTTGATCTT...................................................	21	1	3	0.33	1	0	1	0	0	0
........................................................................................TTCCGACTTTTGCTTGATCT....................................................	20	1	3	0.33	1	0	1	0	0	0
........................................................................................TTCCGACTTTTCCTTGATCTTAA.................................................	23	2	3	0.33	1	0	1	0	0	0
...........................................................................................CGACTTTTACTTGATCTTATCTA..............................................	23	1	3	0.33	1	0	0	0	1	0
........................................................................................TTCCGACTTTTACTTGATCTTT..................................................	22	1	3	0.33	1	0	0	0	1	0
........................................................................................TTCCGACTTTTACTTGATC.....................................................	19	0	3	0.33	1	0	0	0	0	1
........................................................................................TTCCGACTTTTACTTGATCTC...................................................	21	1	3	0.33	1	1	0	0	0	0
.........................................................................................TCCGACTTTTACTTGATCTTA..................................................	21	0	3	0.33	1	0	1	0	0	0
........................................................................................TTCCGACTTTTACTTGATCC....................................................	20	1	3	0.33	1	1	0	0	0	0
........................................................................................TTCCGACTTTTACTTGATTTT...................................................	21	1	3	0.33	1	0	0	0	1	0
........................................................................................TTCCGACCTTTACTTGATCTTC..................................................	22	2	3	0.33	1	0	1	0	0	0
........................................................................................TTCCGACTTTTACTTGATCG....................................................	20	1	3	0.33	1	1	0	0	0	0
.........................................................................................TCCGACTTTTACTTGATCTTAA.................................................	22	1	3	0.33	1	0	1	0	0	0
..................................................AGTTCAAGTAAGGGTAAGG...........................................................................................	19	0	4	0.25	1	0	0	1	0	0
...............................................TAGAGTTCAAGTAAGGGTAAGGAA.........................................................................................	24	1	4	0.25	1	0	0	0	1	0
...............................................TAGGGTTCAAGTAAGGGTAAGG...........................................................................................	22	1	4	0.25	1	0	0	0	1	0
...............................................TAGAGTTCCAGTAAGGGTAAG............................................................................................	21	1	4	0.25	1	0	0	0	1	0
..................................................AGTTCAACTAAGGGTAAGGAGCG.......................................................................................	23	1	4	0.25	1	0	0	1	0	0
...............................................TAGAGTTCAAGTAAGGTTAAGGAG.........................................................................................	24	1	4	0.25	1	0	0	0	1	0
..................................................GGTTCAAGTAAGGGTAAGGAGCG.......................................................................................	23	1	4	0.25	1	0	0	1	0	0
................................................AGAGTTCAAGTAAGGGTAAG............................................................................................	20	0	4	0.25	1	0	0	1	0	0
...............................................TAGAGTTCAAGTAAGCGTAAGGAG.........................................................................................	24	1	5	0.20	1	0	0	0	1	0
...............................................TAGAGTTCAAGTAAGCGTAAGGA..........................................................................................	23	1	5	0.20	1	0	0	1	0	0
...............................................TAGAGTTCAAGTAAGGGTAAAG...........................................................................................	22	1	8	0.13	1	0	1	0	0	0
...............................................TAGAGTTCAAGTAAGGGTAAAGA..........................................................................................	23	1	8	0.13	1	0	0	1	0	0
...............................................TAGAGTTCAAGTAAGGGTAAAGAA.........................................................................................	24	2	8	0.13	1	0	0	0	0	1
...............................................TAGAGTTCAAGTAAGGGTAAA............................................................................................	21	1	9	0.11	1	0	0	1	0	0

CTAAGTGATCGTCGCAGGATCATAAAAATCATATCTTATATGCAAAGTAGAGTTCAAGTAAGGGTAAGGAGCGTTATAAATCGAAATCTTCCGACTTTTACTTGATCTTATCTCGGCGTATAAAAAACTCATAAAAAAAATACAAATCAACATTTTTCTA

***********************************((((((((..((..(((.((((((((((((..((((.(((.........))).)))).)))))))))))).)))..))..)))))))).************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M020

head

M045

female body

V117

male body

V118

embryo

V119

head

........................................................................................TTCCGACTTTTACTTGATCTT...................................................

90.00

270

165

........................................................................................TTCCGACTTTTACTTGATCTTA..................................................

30.00

........................................................................................TTCCGACTTTTACTTGATCT....................................................

21.00

..................................................AGTTCAAGTAAGGGTAAGGAGCG.......................................................................................

6.00

........................................................................................TTCCGACTTTTACTTGATCTTAA.................................................

4.67

...............................................TAGAGTTCAAGTAAGGGTAAGGA..........................................................................................

3.50

..................................................AGTTCAAGTAAGGGTAAGGAGC........................................................................................

3.00

...............................................TAGAGTTCAAGTAAGGGTAAG............................................................................................

1.75

...............................................TAGAGTTCAAGTAAGGGTAAGG...........................................................................................

1.75

.........................................................................................TCCGACTTTTACTTGATCTT...................................................

1.67

...............................................TAGAGTTCAAGTAAGGGTAA.............................................................................................

1.00

......................................................................................GCTTCCGACTTTTACTTGATCTT...................................................

1.00

........................................................................................TTCCGACTTTTAATTGATCTT...................................................

1.00

.......................................................................................CTTCCGACTTTTACTTGATCTT...................................................

1.00

........................................................................................TTCCGACTTTTACTTGATCTTC..................................................

0.67

..................................................AGTTCAAGTAAGGGTAAGGA..........................................................................................

0.50

..................................................AGTTCAAGTAAGGGTAAGGAG.........................................................................................

0.50

........................................................................................TGCCGACTTTTACTTGATCTT...................................................

0.33

........................................................................................TTCCGACTTTTGCTTGATCT....................................................

0.33

........................................................................................TTCCGACTTTTCCTTGATCTTAA.................................................

0.33

...........................................................................................CGACTTTTACTTGATCTTATCTA..............................................

0.33

........................................................................................TTCCGACTTTTACTTGATCTTT..................................................

0.33

........................................................................................TTCCGACTTTTACTTGATC.....................................................

0.33

........................................................................................TTCCGACTTTTACTTGATCTC...................................................

0.33

.........................................................................................TCCGACTTTTACTTGATCTTA..................................................

0.33

........................................................................................TTCCGACTTTTACTTGATCC....................................................

0.33

........................................................................................TTCCGACTTTTACTTGATTTT...................................................

0.33

........................................................................................TTCCGACCTTTACTTGATCTTC..................................................

0.33

........................................................................................TTCCGACTTTTACTTGATCG....................................................

0.33

.........................................................................................TCCGACTTTTACTTGATCTTAA.................................................

0.33

..................................................AGTTCAAGTAAGGGTAAGG...........................................................................................

0.25

...............................................TAGAGTTCAAGTAAGGGTAAGGAA.........................................................................................

0.25

...............................................TAGGGTTCAAGTAAGGGTAAGG...........................................................................................

0.25

...............................................TAGAGTTCCAGTAAGGGTAAG............................................................................................

0.25

..................................................AGTTCAACTAAGGGTAAGGAGCG.......................................................................................

0.25

...............................................TAGAGTTCAAGTAAGGTTAAGGAG.........................................................................................

0.25

..................................................GGTTCAAGTAAGGGTAAGGAGCG.......................................................................................

0.25

................................................AGAGTTCAAGTAAGGGTAAG............................................................................................

0.25

...............................................TAGAGTTCAAGTAAGCGTAAGGAG.........................................................................................

0.20

...............................................TAGAGTTCAAGTAAGCGTAAGGA..........................................................................................

0.20

...............................................TAGAGTTCAAGTAAGGGTAAAG...........................................................................................

0.13

...............................................TAGAGTTCAAGTAAGGGTAAAGA..........................................................................................

0.13

...............................................TAGAGTTCAAGTAAGGGTAAAGAA.........................................................................................

0.13

...............................................TAGAGTTCAAGTAAGGGTAAA............................................................................................

0.11

GATTCACTAGCAGCGTCCTAGTATTTTTAGTATAGAATATACGTTTCATCTCAAGTTCATTCCCATTCCTCGCAATATTTAGCTTTAGAAGGCTGAAAATGAACTAGAATAGAGCCGCATATTTTTTGAGTATTTTTTTTATGTTTAGTTGTAAAAAGAT **********************************((((((((..((..(((.((((((((((((..((((.(((.........))).)))).)))))))))))).)))..))..)))))))).*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V117 male body	V118 embryo
.................................................................................CCTTTGGAATGCTGAAAATGA..........................................................	21	3	4	0.25	1	1	0
........................................................................................................................ATTTCTCGAGTATTTTTTTTATAT................	24	3	6	0.17	1	0	1
...................................................................................TTTGGAATGCTGAAAATG...........................................................	18	2	19	0.05	1	1	0

GATTCACTAGCAGCGTCCTAGTATTTTTAGTATAGAATATACGTTTCATCTCAAGTTCATTCCCATTCCTCGCAATATTTAGCTTTAGAAGGCTGAAAATGAACTAGAATAGAGCCGCATATTTTTTGAGTATTTTTTTTATGTTTAGTTGTAAAAAGAT

************************************((((((((..((..(((.((((((((((((..((((.(((.........))).)))).)))))))))))).)))..))..)))))))).***********************************

Read size

# Mismatch

Hit Count

Total Norm

Total

V117

male body

V118

embryo

.................................................................................CCTTTGGAATGCTGAAAATGA..........................................................

0.25

........................................................................................................................ATTTCTCGAGTATTTTTTTTATAT................

0.17

...................................................................................TTTGGAATGCTGAAAATG...........................................................

0.05

Species	Coordinate	ID	Type	Alignment
droWil2	scf2_1100000004511:3393060-3393219 -	dwi_72	confident	CTAAGTGATCGTCGCAGGATCATAAAAATCATATCTTATATGCAAAGTAGAGTTCAAGTAAGGGTAAGGAGCGTTATAAATCGAAATCTTCCGACTTTTACTTGATCTTATCTCGGCGTATAAAAAACTCATAAAAAAAATACAAATCAACATTTTTCTA

Species

Coordinate

Type

Alignment

droWil2

scf2_1100000004511:3393060-3393219 -

dwi_72

confident

CTAAGTGATCGTCGCAGGATCATAAAAATCATATCTTATATGCAAAGTAGAGTTCAAGTAAGGGTAAGGAGCGTTATAAATCGAAATCTTCCGACTTTTACTTGATCTTATCTCGGCGTATAAAAAACTCATAAAAAAAATACAAATCAACATTTTTCTA

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droWil2

CTAAGTGATCGTCGCAGGATCATAAAAATCATATCTTATATGCAAAGTAGAGTTCAAGTAAGGGTAAGGAGCGTTATAAATCGAAATCTTCCGACTTTTACTTGATCTTATCTCGGCGTATAAAAAACTCATAAAAAAAATACAAATCAACATTTTTCTA	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
........................................................................................TTCCGACTTTTACTTGATCTT...................................................	21	3	90.00	270	165	62	24	11	8
........................................................................................TTCCGACTTTTACTTGATCTTA..................................................	22	3	30.00	90	5	35	15	35	0
........................................................................................TTCCGACTTTTACTTGATCT....................................................	20	3	21.00	63	55	3	4	0	1
..................................................AGTTCAAGTAAGGGTAAGGAGCG.......................................................................................	23	4	6.00	24	0	3	15	6	0
........................................................................................TTCCGACTTTTACTTGATCTTAA.................................................	23	3	4.67	14	2	10	0	1	1
...............................................TAGAGTTCAAGTAAGGGTAAGGA..........................................................................................	23	4	3.50	14	0	0	5	9	0
..................................................AGTTCAAGTAAGGGTAAGGAGC........................................................................................	22	4	3.00	12	0	2	9	0	1
...............................................TAGAGTTCAAGTAAGGGTAAG............................................................................................	21	4	1.75	7	0	0	7	0	0
...............................................TAGAGTTCAAGTAAGGGTAAGG...........................................................................................	22	4	1.75	7	0	0	6	1	0
.........................................................................................TCCGACTTTTACTTGATCTT...................................................	20	3	1.67	5	0	5	0	0	0
...............................................TAGAGTTCAAGTAAGGGTAA.............................................................................................	20	4	1.00	4	0	0	3	1	0
.......................................................................................CTTCCGACTTTTACTTGATCTT...................................................	22	1	1.00	1	0	0	0	1	0
........................................................................................TTCCGACTTTTACTTGATCTTC..................................................	22	3	0.67	2	1	1	0	0	0
..................................................AGTTCAAGTAAGGGTAAGGA..........................................................................................	20	4	0.50	2	0	0	2	0	0
..................................................AGTTCAAGTAAGGGTAAGGAG.........................................................................................	21	4	0.50	2	0	1	1	0	0
...........................................................................................CGACTTTTACTTGATCTTATCTA..............................................	23	3	0.33	1	0	0	0	1	0
........................................................................................TTCCGACTTTTACTTGATCTTT..................................................	22	3	0.33	1	0	0	0	1	0
........................................................................................TTCCGACTTTTACTTGATC.....................................................	19	3	0.33	1	0	0	0	0	1
........................................................................................TTCCGACTTTTACTTGATCTC...................................................	21	3	0.33	1	1	0	0	0	0
.........................................................................................TCCGACTTTTACTTGATCTTA..................................................	21	3	0.33	1	0	1	0	0	0
........................................................................................TTCCGACTTTTACTTGATCC....................................................	20	3	0.33	1	1	0	0	0	0
........................................................................................TTCCGACTTTTACTTGATCG....................................................	20	3	0.33	1	1	0	0	0	0
.........................................................................................TCCGACTTTTACTTGATCTTAA.................................................	22	3	0.33	1	0	1	0	0	0
..................................................AGTTCAAGTAAGGGTAAGG...........................................................................................	19	4	0.25	1	0	0	1	0	0
...............................................TAGAGTTCAAGTAAGGGTAAGGAA.........................................................................................	24	4	0.25	1	0	0	0	1	0
................................................AGAGTTCAAGTAAGGGTAAG............................................................................................	20	4	0.25	1	0	0	1	0	0
...............................................TAGAGTTCAAGTAAGGGTAAA............................................................................................	21	9	0.11	1	0	0	1	0	0

dwi_72

scf2_1100000004511:3393110-3393169 -

confident

Testes-restricted

antisense_to_intron

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-29.8	-29.4	-29.0