dwi_4132

exon [dwil_GLEANR_503:2]; CDS [Dwil\GK21933-cds]; exon [dwil_GLEANR_503:1]; CDS [Dwil\GK21933-cds]; intron [Dwil\GK21933-in]

##################################################----------------------------------------------------################################################## CTTCGTCGGGTACCCGTCTATCGAGGGTGTCGACGAGCCATTCCGGTGGTGTGAGGACCCAATTGAGGGCTGCGGGGTTGGCCTCCGACACATCAATTGTAGATCGGCGCAAAGGGCCTCGCGAAATTGGTCCAGGGCTAGTGTGGGTTGGC ************************************************.........(((((((....(((((((.....))))).))..)))))))...************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M045 female body	V118 embryo	V117 male body	V119 head
...........................TGTCGACGAGCCATTCCGGTGG.......................................................................................................	22	0	6	12.33	74	12	62	0	0
...........................TGTCGACGAGCCATTCCGGTGGTG.....................................................................................................	24	0	5	12.20	61	40	20	1	0
...........................TGTCGACGAGCCATTCCGGTGGTGT....................................................................................................	25	0	5	11.80	59	13	44	2	0
...................................................TGAGGACCCAATTGAGGGCTGCGGG............................................................................	25	0	4	8.75	35	29	5	0	1
...................................................TGAGGACCCAATTGAGGGCTGCGGGG...........................................................................	26	0	3	8.00	24	22	2	0	0
.................................................TGTGAGGACCCAATTGAGGGCTGCGG.............................................................................	26	0	4	3.50	14	12	2	0	0
...........................TGTCGACGAGCCATTCCGGTGGT......................................................................................................	23	0	5	3.40	17	4	13	0	0
...................................................TGAGGACCCAATTGAGGGCTGCGG.............................................................................	24	0	4	3.25	13	6	7	0	0
.............................TCGACGAGCCATTCCGGTGGTGT....................................................................................................	23	0	5	2.20	11	2	8	1	0
...............................................................................................................................TGGTCCAGGGCTAGTGTGGGTTGG.	24	0	4	2.00	8	7	1	0	0
.......................................................GACCCAATTGAGGGCTGCGGGG...........................................................................	22	0	3	1.67	5	5	0	0	0
.........................................................CCCAATTGAGGGCTGCGGGG...........................................................................	20	0	3	1.33	4	4	0	0	0
.................................................TGTGAGGACCCAATTGAGGGCTGCG..............................................................................	25	0	4	1.25	5	2	3	0	0
.............................TCGACGAGCCATTCCGGTGGTGTG...................................................................................................	24	0	5	1.20	6	1	5	0	0
.......................................................GACCCAATTGATGGCTGCGGGGT..........................................................................	23	1	1	1.00	1	0	1	0	0
...........................TGTCGACGAGCCATTCCGGTGGTGTGA..................................................................................................	27	0	5	1.00	5	5	0	0	0
....................................................GAGGACCCAATTGAGGGCTGCGGGG...........................................................................	25	0	3	1.00	3	3	0	0	0
...........................TGTCGACGAGCCATTCCGGTGGTGTG...................................................................................................	26	0	5	1.00	5	1	4	0	0
........................................TTCCGGTGGTGTGAGGACCCAATTG.......................................................................................	25	0	3	1.00	3	2	1	0	0
..............................................................................................................................TTGGTCCAGGGCTAGTGTGGGTTG..	24	0	4	1.00	4	3	1	0	0
.......................................ATTCCGGTGGTGTGAGGACCCAATAAA......................................................................................	27	2	1	1.00	1	0	1	0	0
...........................TGTCGACGAGCCATTCCGGTGGTGTA...................................................................................................	26	1	5	1.00	5	0	5	0	0
...........................TGTCGACGAGCCATTCCGGTGGTGTGT..................................................................................................	27	1	5	0.80	4	1	2	1	0
...............................................................................................................................TGGTCCAGGGCTAGTGTGGGTTG..	23	0	4	0.75	3	3	0	0	0
..............................CGACGAGCCATTCCGGTGGTGTGAGG................................................................................................	26	0	4	0.75	3	3	0	0	0
.......................................................GACCCAATTGAGGGCTGCGG.............................................................................	20	0	4	0.75	3	3	0	0	0
...........................................................................................................................AAATTGGTCAAGGGTTAGT..........	19	2	4	0.75	3	0	0	3	0
...............................................GGTGTGAGGACCCAATTGAGGGCT.................................................................................	24	0	4	0.75	3	0	1	0	2
..............................CGACGAGCCATTCCGGTGGTGT....................................................................................................	22	0	5	0.60	3	0	3	0	0
............................GTCGACGAGCCATTCCGGTGGTGTG...................................................................................................	25	0	5	0.60	3	3	0	0	0
.............................TCGACGAGCCATTCCGGTGGTGTGA..................................................................................................	25	0	5	0.60	3	1	2	0	0
..............................CGACGAGCCATTCCGGTGGTGTGAGT................................................................................................	26	1	5	0.60	3	0	3	0	0
..............................CGACGAGCCATTCCGGTGGTGTG...................................................................................................	23	0	5	0.60	3	1	2	0	0
............................GTCGACGAGCCATTCCGGTGGTG.....................................................................................................	23	0	5	0.60	3	3	0	0	0
......................................................GGACCCAATTGAGGGCTGCGGG............................................................................	22	0	4	0.50	2	2	0	0	0
..............................................................................................................................TTGGTCCAGGGCTAGTGTGGGTTGGC	26	0	4	0.50	2	1	1	0	0
....................................................GAGGACCCAATTGAGGGCTGCGGG............................................................................	24	0	4	0.50	2	2	0	0	0
.....................................CCATTCCGGTGGTGTGAGGACCCAA..........................................................................................	25	0	4	0.50	2	2	0	0	0
.................................................TGTGAGGACCCAATTGAGGGCT.................................................................................	22	0	4	0.50	2	0	2	0	0
...................................................TGAGGACCCAATTGAGGGCTGC...............................................................................	22	0	4	0.50	2	1	1	0	0
.......................................................GACCCAATTGAGGGCTGCGGG............................................................................	21	0	4	0.50	2	2	0	0	0
.................................................TGTGAGGACCCAATTGAGGGCTGC...............................................................................	24	0	4	0.50	2	1	1	0	0
..............................................TGGTGTGAGGACCCAATTGAGG....................................................................................	22	0	2	0.50	1	1	0	0	0
..............................................TGGTGTGAGGACCCAATTGAGGGC..................................................................................	24	0	2	0.50	1	0	1	0	0
........................................TTCCGGTGGTGTGAGGACCCA...........................................................................................	21	0	4	0.50	2	1	1	0	0
.............................TCGACGAGCCATTCCGGTGG.......................................................................................................	20	0	6	0.50	3	0	3	0	0
............................GTCGACGAGCCATTCCGGTGGTGT....................................................................................................	24	0	5	0.40	2	2	0	0	0
.............................TCGACGAGCCATTCCGGTGGT......................................................................................................	21	0	5	0.40	2	0	2	0	0
...........................TGTCGACGAGCCATTCCGGTGGTGTT...................................................................................................	26	1	5	0.40	2	0	2	0	0
...........................TGTCGACGAGCCATTCCGG..........................................................................................................	19	0	8	0.38	3	0	3	0	0
...........................TGTCGACGAGCCATTCCGGTGGTT.....................................................................................................	24	1	6	0.33	2	0	2	0	0
...........................................................CAATTGAGGGCTGCGGGG...........................................................................	18	0	3	0.33	1	1	0	0	0
....................................GCCATTCCGGTGGTGTGAGGACCCAATT........................................................................................	28	0	3	0.33	1	1	0	0	0
......................................................GGACCCAATTGAGGGCTGCGGGG...........................................................................	23	0	3	0.33	1	1	0	0	0
....................................GCCATTCCGGTGGTGTGAGGACCCAAT.........................................................................................	27	0	3	0.33	1	1	0	0	0
.................................................TGTGAGGACCCAATTGAGGGCTG................................................................................	23	0	4	0.25	1	1	0	0	0
.........................................................CCCAATTGAGGGCTGCGGG............................................................................	19	0	4	0.25	1	1	0	0	0
...................................................TGAGGACCCAATTGAGGGCTGCG..............................................................................	23	0	4	0.25	1	1	0	0	0
.................................................TGTGAGGACCCAATTGAGGGCTGCGGT............................................................................	27	1	4	0.25	1	0	1	0	0
................................................GTGTGAGGACCCAATTGAGGGCTG................................................................................	24	0	4	0.25	1	1	0	0	0
.................................CGAGCCATTCCGGTGGTGTGAGG................................................................................................	23	0	4	0.25	1	0	1	0	0
.................................................TGTGAGGACCCAATTGAGG....................................................................................	19	0	4	0.25	1	0	1	0	0
..................................................GTGAGGACCCAATTGAGGGCTGCGG.............................................................................	25	0	4	0.25	1	1	0	0	0
..................................................................................................................................TCCAGTGCTAGTGTGGGTTGGC	22	1	4	0.25	1	0	1	0	0
.............................TCGACGAGCCATTCCGGTGGTGTGAG.................................................................................................	26	0	4	0.25	1	0	1	0	0
...............................................................................................................................TCGTCCAGGGCTAGTGTGGGTTGG.	24	1	4	0.25	1	0	1	0	0
....................................................GAGGACCCAATTGAGGGCTGCGG.............................................................................	23	0	4	0.25	1	1	0	0	0
.................................................TGTGAGGACCCAATTGAGGGC..................................................................................	21	0	4	0.25	1	1	0	0	0
.................................................TGTGAGGACCCAATTGAGGGCTGCGGG............................................................................	27	0	4	0.25	1	1	0	0	0
....................................GCCATTCCGGTGGTGTGAGGACCC............................................................................................	24	0	4	0.25	1	1	0	0	0
...............................................................................................................................TGGTCCAGGGCTAGTGTGGGTTGGC	25	0	4	0.25	1	1	0	0	0
...............................................................................................................................TGGTCCAGGGCTAGTGTGGGTT...	22	0	4	0.25	1	1	0	0	0
....................................................................................................................................CAGGGCTTGTATGGGTAGGC	20	3	8	0.25	2	2	0	0	0
...................................................TGAGGACCCAATTGAGGGCT.................................................................................	20	0	4	0.25	1	1	0	0	0
..............................................................................................................................TTGGTCCAGGGCTAGTGTGGGTTGG.	25	0	4	0.25	1	1	0	0	0
....................................GCCATTCCGGTGGTGTGAGGA...............................................................................................	21	0	4	0.25	1	0	1	0	0
..................................................................................................................................TCCAGGGCTAGTGTGGGTTGG.	21	0	4	0.25	1	1	0	0	0
....................................GCCATTCCGGTGGTGTGAGGACCCA...........................................................................................	25	0	4	0.25	1	1	0	0	0
.............................TCGACGAGCCATTCCGGTGGTGTGAGG................................................................................................	27	0	4	0.25	1	1	0	0	0
................................................................................................................................GGTCCAGGGCTAGTGTGGGTTGGC	24	0	4	0.25	1	1	0	0	0
..............................................................................................................................TTGGTCCAGGGCTAGTGTGGGTT...	23	0	4	0.25	1	0	1	0	0
..................................GAGCCATTCCGGTGGTGTGAGGAC..............................................................................................	24	0	4	0.25	1	1	0	0	0
..............................................................................................................................TTGGTCCAGGGCTAGTGTGGGTTAAG	26	3	4	0.25	1	0	1	0	0
....................................GCCATTCCGGTGGTGTGAGGACCCAA..........................................................................................	26	0	4	0.25	1	1	0	0	0
...................................AGCCATTCCGGTGGTGTGAGGACC.............................................................................................	24	0	4	0.25	1	1	0	0	0
.................................................TGTGAGGACCCAATTGAGGGCTGCT..............................................................................	25	1	4	0.25	1	1	0	0	0
.................................................TGTGAGGACCCAAGTGAGGGCTGC...............................................................................	24	1	4	0.25	1	0	1	0	0
...............................................GGTGTGAGGACCCAATTGAGGGC..................................................................................	23	0	4	0.25	1	0	1	0	0
..............................................................................................................................TTGATCCAGGGCTAGTGTGGGTT...	23	1	4	0.25	1	1	0	0	0
..............................CGACGAGCCATTCCGGTGGTGTGAG.................................................................................................	25	0	4	0.25	1	1	0	0	0
...........................TGTCGACGAGCAATTCCGGTGGTG.....................................................................................................	24	1	5	0.20	1	1	0	0	0
...............................GACGAGCCATTCCGGTGGTG.....................................................................................................	20	0	5	0.20	1	0	1	0	0
...........................TGTCGACGAGCCATTCCGGTGGTTAG...................................................................................................	26	2	5	0.20	1	1	0	0	0
...........................TGTCGACCAGCCATTCCGGTGGTGT....................................................................................................	25	1	5	0.20	1	0	1	0	0
..............................CGACGAGCCATTCCGGTGGTG.....................................................................................................	21	0	5	0.20	1	1	0	0	0
..............................CGACCAGCCATTCCGGTGGTGTGT..................................................................................................	24	2	5	0.20	1	0	1	0	0
..............................CGACGAGCCATTCCGGTGGTGTGA..................................................................................................	24	0	5	0.20	1	1	0	0	0
...........................TGTCGACGAGCCATTCCGGTGGTGAG...................................................................................................	26	1	5	0.20	1	1	0	0	0
...........................TGTCGACGAGCCATTCCGGTGGTGTTA..................................................................................................	27	1	5	0.20	1	0	1	0	0
...........................TGTCGACGAGCCACTCCGGTGGTG.....................................................................................................	24	1	5	0.20	1	0	1	0	0
...........................TGTCGACGAGCCATTCCTGTGGTG.....................................................................................................	24	1	5	0.20	1	1	0	0	0
...........................TGTCGACGAGCCATTCCGGTGGTGTATA.................................................................................................	28	3	5	0.20	1	0	0	1	0
...........................TGTCGACGAGCCCTTCCGGTGGTGT....................................................................................................	25	1	5	0.20	1	0	1	0	0
...........................TGTCGACGAGCCATTCCGGTGGTGTTAC.................................................................................................	28	2	5	0.20	1	0	1	0	0
..................................................CTGAGTACCCAATTAAGGGC..................................................................................	20	3	5	0.20	1	1	0	0	0
...........................TGTCGACTAGCCATTCCGGTGGTGT....................................................................................................	25	1	5	0.20	1	0	1	0	0
...........................TGTCGACGAGCCATTCCGGTGGTGA....................................................................................................	25	1	5	0.20	1	0	1	0	0
...........................TGTCGACGAGCCATTCCCGTGGTGT....................................................................................................	25	1	5	0.20	1	0	1	0	0
...........................TGACGACGAGCCATTCCGGTGGTG.....................................................................................................	24	1	5	0.20	1	0	1	0	0
...........................TGTCTACGAGCCATTCCGGTGGTG.....................................................................................................	24	1	5	0.20	1	0	1	0	0
...........................TGTCGACGAGCCATTCCGGTGGTGTTC..................................................................................................	27	2	5	0.20	1	1	0	0	0
...........................TGTCGACGAGCCACTCCGGTGG.......................................................................................................	22	1	6	0.17	1	0	1	0	0
..............................................................................................................................TTGGTCCAGGCCTAGTGTGGGTTGG.	25	1	6	0.17	1	0	0	1	0
...........................TGTCGACTAGCCATTCCGGTGG.......................................................................................................	22	1	6	0.17	1	0	1	0	0
...........................TGTCGACGAGCCATTCCGGTGGTGG....................................................................................................	25	1	6	0.17	1	0	1	0	0
..........................GTGTCGACGAGCCATTCCGGTGG.......................................................................................................	23	0	6	0.17	1	1	0	0	0
.............................TCGACGAGCCATTCCGGTGC.......................................................................................................	20	1	7	0.14	1	1	0	0	0
....................................................................................................................................CAGGGCTTGTATGGGTAGG.	19	3	16	0.06	1	1	0	0	0
...................................................................................................TAGATCGGCGCAAAGGGCCTCG...............................	22	0	18	0.06	1	1	0	0	0

##################################################----------------------------------------------------##################################################
CTTCGTCGGGTACCCGTCTATCGAGGGTGTCGACGAGCCATTCCGGTGGTGTGAGGACCCAATTGAGGGCTGCGGGGTTGGCCTCCGACACATCAATTGTAGATCGGCGCAAAGGGCCTCGCGAAATTGGTCCAGGGCTAGTGTGGGTTGGC
**************************************************.........(((((((....(((((((.....))))).))..)))))))...**************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M045
female body

V118
embryo

V117
male body

V119
head

...........................TGTCGACGAGCCATTCCGGTGG.......................................................................................................

12.33

...........................TGTCGACGAGCCATTCCGGTGGTG.....................................................................................................

12.20

...........................TGTCGACGAGCCATTCCGGTGGTGT....................................................................................................

11.80

...................................................TGAGGACCCAATTGAGGGCTGCGGG............................................................................

8.75

...................................................TGAGGACCCAATTGAGGGCTGCGGGG...........................................................................

8.00

.................................................TGTGAGGACCCAATTGAGGGCTGCGG.............................................................................

3.50

...........................TGTCGACGAGCCATTCCGGTGGT......................................................................................................

3.40

...................................................TGAGGACCCAATTGAGGGCTGCGG.............................................................................

3.25

.............................TCGACGAGCCATTCCGGTGGTGT....................................................................................................

2.20

...............................................................................................................................TGGTCCAGGGCTAGTGTGGGTTGG.

2.00

.......................................................GACCCAATTGAGGGCTGCGGGG...........................................................................

1.67

.........................................................CCCAATTGAGGGCTGCGGGG...........................................................................

1.33

.................................................TGTGAGGACCCAATTGAGGGCTGCG..............................................................................

1.25

.............................TCGACGAGCCATTCCGGTGGTGTG...................................................................................................

1.20

.......................................................GACCCAATTGATGGCTGCGGGGT..........................................................................

1.00

...........................TGTCGACGAGCCATTCCGGTGGTGTGA..................................................................................................

1.00

....................................................GAGGACCCAATTGAGGGCTGCGGGG...........................................................................

1.00

...........................TGTCGACGAGCCATTCCGGTGGTGTG...................................................................................................

1.00

........................................TTCCGGTGGTGTGAGGACCCAATTG.......................................................................................

1.00

..............................................................................................................................TTGGTCCAGGGCTAGTGTGGGTTG..

1.00

.......................................ATTCCGGTGGTGTGAGGACCCAATAAA......................................................................................

1.00

...........................TGTCGACGAGCCATTCCGGTGGTGTA...................................................................................................

1.00

...........................TGTCGACGAGCCATTCCGGTGGTGTGT..................................................................................................

0.80

...............................................................................................................................TGGTCCAGGGCTAGTGTGGGTTG..

0.75

..............................CGACGAGCCATTCCGGTGGTGTGAGG................................................................................................

0.75

.......................................................GACCCAATTGAGGGCTGCGG.............................................................................

0.75

...........................................................................................................................AAATTGGTCAAGGGTTAGT..........

0.75

...............................................GGTGTGAGGACCCAATTGAGGGCT.................................................................................

0.75

..............................CGACGAGCCATTCCGGTGGTGT....................................................................................................

0.60

............................GTCGACGAGCCATTCCGGTGGTGTG...................................................................................................

0.60

.............................TCGACGAGCCATTCCGGTGGTGTGA..................................................................................................

0.60

..............................CGACGAGCCATTCCGGTGGTGTGAGT................................................................................................

0.60

..............................CGACGAGCCATTCCGGTGGTGTG...................................................................................................

0.60

............................GTCGACGAGCCATTCCGGTGGTG.....................................................................................................

0.60

......................................................GGACCCAATTGAGGGCTGCGGG............................................................................

0.50

..............................................................................................................................TTGGTCCAGGGCTAGTGTGGGTTGGC

0.50

....................................................GAGGACCCAATTGAGGGCTGCGGG............................................................................

0.50

.....................................CCATTCCGGTGGTGTGAGGACCCAA..........................................................................................

0.50

.................................................TGTGAGGACCCAATTGAGGGCT.................................................................................

0.50

...................................................TGAGGACCCAATTGAGGGCTGC...............................................................................

0.50

.......................................................GACCCAATTGAGGGCTGCGGG............................................................................

0.50

.................................................TGTGAGGACCCAATTGAGGGCTGC...............................................................................

0.50

..............................................TGGTGTGAGGACCCAATTGAGG....................................................................................

0.50

..............................................TGGTGTGAGGACCCAATTGAGGGC..................................................................................

0.50

........................................TTCCGGTGGTGTGAGGACCCA...........................................................................................

0.50

.............................TCGACGAGCCATTCCGGTGG.......................................................................................................

0.50

............................GTCGACGAGCCATTCCGGTGGTGT....................................................................................................

0.40

.............................TCGACGAGCCATTCCGGTGGT......................................................................................................

0.40

...........................TGTCGACGAGCCATTCCGGTGGTGTT...................................................................................................

0.40

...........................TGTCGACGAGCCATTCCGG..........................................................................................................

0.38

...........................TGTCGACGAGCCATTCCGGTGGTT.....................................................................................................

0.33

...........................................................CAATTGAGGGCTGCGGGG...........................................................................

0.33

....................................GCCATTCCGGTGGTGTGAGGACCCAATT........................................................................................

0.33

......................................................GGACCCAATTGAGGGCTGCGGGG...........................................................................

0.33

....................................GCCATTCCGGTGGTGTGAGGACCCAAT.........................................................................................

0.33

.................................................TGTGAGGACCCAATTGAGGGCTG................................................................................

0.25

.........................................................CCCAATTGAGGGCTGCGGG............................................................................

0.25

...................................................TGAGGACCCAATTGAGGGCTGCG..............................................................................

0.25

.................................................TGTGAGGACCCAATTGAGGGCTGCGGT............................................................................

0.25

................................................GTGTGAGGACCCAATTGAGGGCTG................................................................................

0.25

.................................CGAGCCATTCCGGTGGTGTGAGG................................................................................................

0.25

.................................................TGTGAGGACCCAATTGAGG....................................................................................

0.25

..................................................GTGAGGACCCAATTGAGGGCTGCGG.............................................................................

0.25

..................................................................................................................................TCCAGTGCTAGTGTGGGTTGGC

0.25

.............................TCGACGAGCCATTCCGGTGGTGTGAG.................................................................................................

0.25

...............................................................................................................................TCGTCCAGGGCTAGTGTGGGTTGG.

0.25

....................................................GAGGACCCAATTGAGGGCTGCGG.............................................................................

0.25

.................................................TGTGAGGACCCAATTGAGGGC..................................................................................

0.25

.................................................TGTGAGGACCCAATTGAGGGCTGCGGG............................................................................

0.25

....................................GCCATTCCGGTGGTGTGAGGACCC............................................................................................

0.25

...............................................................................................................................TGGTCCAGGGCTAGTGTGGGTTGGC

0.25

...............................................................................................................................TGGTCCAGGGCTAGTGTGGGTT...

0.25

....................................................................................................................................CAGGGCTTGTATGGGTAGGC

0.25

...................................................TGAGGACCCAATTGAGGGCT.................................................................................

0.25

..............................................................................................................................TTGGTCCAGGGCTAGTGTGGGTTGG.

0.25

....................................GCCATTCCGGTGGTGTGAGGA...............................................................................................

0.25

..................................................................................................................................TCCAGGGCTAGTGTGGGTTGG.

0.25

....................................GCCATTCCGGTGGTGTGAGGACCCA...........................................................................................

0.25

.............................TCGACGAGCCATTCCGGTGGTGTGAGG................................................................................................

0.25

................................................................................................................................GGTCCAGGGCTAGTGTGGGTTGGC

0.25

..............................................................................................................................TTGGTCCAGGGCTAGTGTGGGTT...

0.25

..................................GAGCCATTCCGGTGGTGTGAGGAC..............................................................................................

0.25

..............................................................................................................................TTGGTCCAGGGCTAGTGTGGGTTAAG

0.25

....................................GCCATTCCGGTGGTGTGAGGACCCAA..........................................................................................

0.25

...................................AGCCATTCCGGTGGTGTGAGGACC.............................................................................................

0.25

.................................................TGTGAGGACCCAATTGAGGGCTGCT..............................................................................

0.25

.................................................TGTGAGGACCCAAGTGAGGGCTGC...............................................................................

0.25

...............................................GGTGTGAGGACCCAATTGAGGGC..................................................................................

0.25

..............................................................................................................................TTGATCCAGGGCTAGTGTGGGTT...

0.25

..............................CGACGAGCCATTCCGGTGGTGTGAG.................................................................................................

0.25

...........................TGTCGACGAGCAATTCCGGTGGTG.....................................................................................................

0.20

...............................GACGAGCCATTCCGGTGGTG.....................................................................................................

0.20

...........................TGTCGACGAGCCATTCCGGTGGTTAG...................................................................................................

0.20

...........................TGTCGACCAGCCATTCCGGTGGTGT....................................................................................................

0.20

..............................CGACGAGCCATTCCGGTGGTG.....................................................................................................

0.20

..............................CGACCAGCCATTCCGGTGGTGTGT..................................................................................................

0.20

..............................CGACGAGCCATTCCGGTGGTGTGA..................................................................................................

0.20

...........................TGTCGACGAGCCATTCCGGTGGTGAG...................................................................................................

0.20

...........................TGTCGACGAGCCATTCCGGTGGTGTTA..................................................................................................

0.20

...........................TGTCGACGAGCCACTCCGGTGGTG.....................................................................................................

0.20

...........................TGTCGACGAGCCATTCCTGTGGTG.....................................................................................................

0.20

...........................TGTCGACGAGCCATTCCGGTGGTGTATA.................................................................................................

0.20

...........................TGTCGACGAGCCCTTCCGGTGGTGT....................................................................................................

0.20

...........................TGTCGACGAGCCATTCCGGTGGTGTTAC.................................................................................................

0.20

..................................................CTGAGTACCCAATTAAGGGC..................................................................................

0.20

...........................TGTCGACTAGCCATTCCGGTGGTGT....................................................................................................

0.20

...........................TGTCGACGAGCCATTCCGGTGGTGA....................................................................................................

0.20

...........................TGTCGACGAGCCATTCCCGTGGTGT....................................................................................................

0.20

...........................TGACGACGAGCCATTCCGGTGGTG.....................................................................................................

0.20

...........................TGTCTACGAGCCATTCCGGTGGTG.....................................................................................................

0.20

...........................TGTCGACGAGCCATTCCGGTGGTGTTC..................................................................................................

0.20

...........................TGTCGACGAGCCACTCCGGTGG.......................................................................................................

0.17

..............................................................................................................................TTGGTCCAGGCCTAGTGTGGGTTGG.

0.17

...........................TGTCGACTAGCCATTCCGGTGG.......................................................................................................

0.17

...........................TGTCGACGAGCCATTCCGGTGGTGG....................................................................................................

0.17

..........................GTGTCGACGAGCCATTCCGGTGG.......................................................................................................

0.17

.............................TCGACGAGCCATTCCGGTGC.......................................................................................................

0.14

....................................................................................................................................CAGGGCTTGTATGGGTAGG.

0.06

...................................................................................................TAGATCGGCGCAAAGGGCCTCG...............................

0.06

GAAGCAGCCCATGGGCAGATAGCTCCCACAGCTGCTCGGTAAGGCCACCACACTCCTGGGTTAACTCCCGACGCCCCAACCGGAGGCTGTGTAGTTAACATCTAGCCGCGTTTCCCGGAGCGCTTTAACCAGGTCCCGATCACACCCAACCG ************************************************.........(((((((....(((((((.....))))).))..)))))))...************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M045 female body	V118 embryo	V117 male body
........................................................................GGCCCAAACGGAGGTTGTG.............................................................	19	3	17	0.82	14	12	0	2
...................................TCGGTAAGGCCACCACACTCCTGGGT...........................................................................................	26	0	4	0.75	3	2	1	0
........................CCCACAGCTGCTCGGTAAGGCC..........................................................................................................	22	0	6	0.50	3	3	0	0
.........................CCACAGCTGCTCGGTAAGGCC..........................................................................................................	21	0	6	0.50	3	3	0	0
.............................AGCTGCTCGGTAAGGCCACCA......................................................................................................	21	0	5	0.40	2	2	0	0
.........................CCACAGCTGCTCGGTAAGGCCACCAC.....................................................................................................	26	0	3	0.33	1	1	0	0
......................................GTAAGGCCACCACACTCCTGGGT...........................................................................................	23	0	4	0.25	1	1	0	0
.................................GCTCGGTAAGGCCACCACACTCCT...............................................................................................	24	0	4	0.25	1	1	0	0
.....................................GGTAAGGCCACCACACTCCTGGG............................................................................................	23	0	4	0.25	1	1	0	0
..........................CACAGCTGCTCGGTAAGGCCACCAC.....................................................................................................	25	0	5	0.20	1	1	0	0
...........................ACAGCTGCTCGGTAAGGCCACCA......................................................................................................	23	0	5	0.20	1	1	0	0
..............................GCTGCTCGGTAAGGCCACCAC.....................................................................................................	21	0	5	0.20	1	1	0	0
...........................ACAACTGCTCGGTAAGGCCACCACACT..................................................................................................	27	1	5	0.20	1	1	0	0
...........................ACAGCTGCTCGGTAAGGCCACCACACT..................................................................................................	27	0	5	0.20	1	1	0	0
.............................AGCTGCTCGGTAAGGCCACCACAC...................................................................................................	24	0	5	0.20	1	1	0	0
.............................AGCTGCTCGGTAAGGCCACCACC....................................................................................................	23	1	6	0.17	1	1	0	0
.................................GCTCCGCAAGGCCCCCACACTCC................................................................................................	23	3	6	0.17	1	1	0	0
.................................GCTCCGCAAGGCCCCCACACTC.................................................................................................	22	3	6	0.17	1	1	0	0
..........................CACAGCTGCTCGGTAAGGCC..........................................................................................................	20	0	8	0.13	1	1	0	0
..................................CTCCGCAAGGCCCCCACAC...................................................................................................	19	3	19	0.05	1	1	0	0
..................................................CGCGCCTGGGTTAACGCC....................................................................................	18	3	20	0.05	1	1	0	0

GAAGCAGCCCATGGGCAGATAGCTCCCACAGCTGCTCGGTAAGGCCACCACACTCCTGGGTTAACTCCCGACGCCCCAACCGGAGGCTGTGTAGTTAACATCTAGCCGCGTTTCCCGGAGCGCTTTAACCAGGTCCCGATCACACCCAACCG
**************************************************.........(((((((....(((((((.....))))).))..)))))))...**************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M045
female body

V118
embryo

V117
male body

........................................................................GGCCCAAACGGAGGTTGTG.............................................................

0.82

...................................TCGGTAAGGCCACCACACTCCTGGGT...........................................................................................

0.75

........................CCCACAGCTGCTCGGTAAGGCC..........................................................................................................

0.50

.........................CCACAGCTGCTCGGTAAGGCC..........................................................................................................

0.50

.............................AGCTGCTCGGTAAGGCCACCA......................................................................................................

0.40

.........................CCACAGCTGCTCGGTAAGGCCACCAC.....................................................................................................

0.33

......................................GTAAGGCCACCACACTCCTGGGT...........................................................................................

0.25

.................................GCTCGGTAAGGCCACCACACTCCT...............................................................................................

0.25

.....................................GGTAAGGCCACCACACTCCTGGG............................................................................................

0.25

..........................CACAGCTGCTCGGTAAGGCCACCAC.....................................................................................................

0.20

...........................ACAGCTGCTCGGTAAGGCCACCA......................................................................................................

0.20

..............................GCTGCTCGGTAAGGCCACCAC.....................................................................................................

0.20

...........................ACAACTGCTCGGTAAGGCCACCACACT..................................................................................................

0.20

...........................ACAGCTGCTCGGTAAGGCCACCACACT..................................................................................................

0.20

.............................AGCTGCTCGGTAAGGCCACCACAC...................................................................................................

0.20

.............................AGCTGCTCGGTAAGGCCACCACC....................................................................................................

0.17

.................................GCTCCGCAAGGCCCCCACACTCC................................................................................................

0.17

.................................GCTCCGCAAGGCCCCCACACTC.................................................................................................

0.17

..........................CACAGCTGCTCGGTAAGGCC..........................................................................................................

0.13

..................................CTCCGCAAGGCCCCCACAC...................................................................................................

0.05

..................................................CGCGCCTGGGTTAACGCC....................................................................................

0.05

Species	Coordinate	ID	Alignment
droWil2	scf2_1100000004969:264877-265028 -	dwi_4132	CTTCGTCGGGTACCCGTCTATCGAGGGTGTCGACGAGCCATTCCGGTGGTGTGAGGACCCAATTGAGGGCTGCGGGGTTGGCCTCCGACACATCAATTGTAGATCGGCGCAAAGGGCCTCGCGAAATTGGTCCAGGGCTAGTGTGGGTTGGC

Species

Coordinate

Alignment

droWil2

scf2_1100000004969:264877-265028 -

dwi_4132

CTTCGTCGGGTACCCGTCTATCGAGGGTGTCGACGAGCCATTCCGGTGGTGTGAGGACCCAATTGAGGGCTGCGGGGTTGGCCTCCGACACATCAATTGTAGATCGGCGCAAAGGGCCTCGCGAAATTGGTCCAGGGCTAGTGTGGGTTGGC

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droWil2

CTTCGTCGGGTACCCGTCTATCGAGGGTGTCGACGAGCCATTCCGGTGGTGTGAGGACCCAATTGAGGGCTGCGGGGTTGGCCTCCGACACATCAATTGTAGATCGGCGCAAAGGGCCTCGCGAAATTGGTCCAGGGCTAGTGTGGGTTGGC	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
...........................TGTCGACGAGCCATTCCGGTGG.......................................................................................................	22	6	12.33	74	0	12	0	62	0
...........................TGTCGACGAGCCATTCCGGTGGTG.....................................................................................................	24	5	12.20	61	0	40	1	20	0
...........................TGTCGACGAGCCATTCCGGTGGTGT....................................................................................................	25	5	11.80	59	0	13	2	44	0
...................................................TGAGGACCCAATTGAGGGCTGCGGG............................................................................	25	4	8.75	35	0	29	0	5	1
...................................................TGAGGACCCAATTGAGGGCTGCGGGG...........................................................................	26	3	8.00	24	0	22	0	2	0
.................................................TGTGAGGACCCAATTGAGGGCTGCGG.............................................................................	26	4	3.50	14	0	12	0	2	0
...........................TGTCGACGAGCCATTCCGGTGGT......................................................................................................	23	5	3.40	17	0	4	0	13	0
...................................................TGAGGACCCAATTGAGGGCTGCGG.............................................................................	24	4	3.25	13	0	6	0	7	0
.............................TCGACGAGCCATTCCGGTGGTGT....................................................................................................	23	5	2.20	11	0	2	1	8	0
...............................................................................................................................TGGTCCAGGGCTAGTGTGGGTTGG.	24	4	2.00	8	0	7	0	1	0
.......................................................GACCCAATTGAGGGCTGCGGGG...........................................................................	22	3	1.67	5	0	5	0	0	0
.........................................................CCCAATTGAGGGCTGCGGGG...........................................................................	20	3	1.33	4	0	4	0	0	0
.................................................TGTGAGGACCCAATTGAGGGCTGCG..............................................................................	25	4	1.25	5	0	2	0	3	0
.............................TCGACGAGCCATTCCGGTGGTGTG...................................................................................................	24	5	1.20	6	0	1	0	5	0
...........................TGTCGACGAGCCATTCCGGTGGTGTGA..................................................................................................	27	5	1.00	5	0	5	0	0	0
....................................................GAGGACCCAATTGAGGGCTGCGGGG...........................................................................	25	3	1.00	3	0	3	0	0	0
...........................TGTCGACGAGCCATTCCGGTGGTGTG...................................................................................................	26	5	1.00	5	0	1	0	4	0
........................................TTCCGGTGGTGTGAGGACCCAATTG.......................................................................................	25	3	1.00	3	0	2	0	1	0
..............................................................................................................................TTGGTCCAGGGCTAGTGTGGGTTG..	24	4	1.00	4	0	3	0	1	0
...........................TGTCGACGAGCCATTCCGGTGGTGTA...................................................................................................	26	5	1.00	5	0	0	0	5	0
...........................TGTCGACGAGCCATTCCGGTGGTGTGT..................................................................................................	27	5	0.80	4	0	1	1	2	0
...............................................................................................................................TGGTCCAGGGCTAGTGTGGGTTG..	23	4	0.75	3	0	3	0	0	0
..............................CGACGAGCCATTCCGGTGGTGTGAGG................................................................................................	26	4	0.75	3	0	3	0	0	0
.......................................................GACCCAATTGAGGGCTGCGG.............................................................................	20	4	0.75	3	0	3	0	0	0
...............................................GGTGTGAGGACCCAATTGAGGGCT.................................................................................	24	4	0.75	3	0	0	0	1	2
..............................CGACGAGCCATTCCGGTGGTGT....................................................................................................	22	5	0.60	3	0	0	0	3	0
............................GTCGACGAGCCATTCCGGTGGTGTG...................................................................................................	25	5	0.60	3	0	3	0	0	0
.............................TCGACGAGCCATTCCGGTGGTGTGA..................................................................................................	25	5	0.60	3	0	1	0	2	0
..............................CGACGAGCCATTCCGGTGGTGTGAGT................................................................................................	26	5	0.60	3	0	0	0	3	0
..............................CGACGAGCCATTCCGGTGGTGTG...................................................................................................	23	5	0.60	3	0	1	0	2	0
............................GTCGACGAGCCATTCCGGTGGTG.....................................................................................................	23	5	0.60	3	0	3	0	0	0
......................................................GGACCCAATTGAGGGCTGCGGG............................................................................	22	4	0.50	2	0	2	0	0	0
..............................................................................................................................TTGGTCCAGGGCTAGTGTGGGTTGGC	26	4	0.50	2	0	1	0	1	0
....................................................GAGGACCCAATTGAGGGCTGCGGG............................................................................	24	4	0.50	2	0	2	0	0	0
.....................................CCATTCCGGTGGTGTGAGGACCCAA..........................................................................................	25	4	0.50	2	0	2	0	0	0
.................................................TGTGAGGACCCAATTGAGGGCT.................................................................................	22	4	0.50	2	0	0	0	2	0
...................................................TGAGGACCCAATTGAGGGCTGC...............................................................................	22	4	0.50	2	0	1	0	1	0
.......................................................GACCCAATTGAGGGCTGCGGG............................................................................	21	4	0.50	2	0	2	0	0	0
.................................................TGTGAGGACCCAATTGAGGGCTGC...............................................................................	24	4	0.50	2	0	1	0	1	0
..............................................TGGTGTGAGGACCCAATTGAGG....................................................................................	22	2	0.50	1	0	1	0	0	0
..............................................TGGTGTGAGGACCCAATTGAGGGC..................................................................................	24	2	0.50	1	0	0	0	1	0
........................................TTCCGGTGGTGTGAGGACCCA...........................................................................................	21	4	0.50	2	0	1	0	1	0
.............................TCGACGAGCCATTCCGGTGG.......................................................................................................	20	6	0.50	3	0	0	0	3	0
............................GTCGACGAGCCATTCCGGTGGTGT....................................................................................................	24	5	0.40	2	0	2	0	0	0
.............................TCGACGAGCCATTCCGGTGGT......................................................................................................	21	5	0.40	2	0	0	0	2	0
...........................TGTCGACGAGCCATTCCGGTGGTGTT...................................................................................................	26	5	0.40	2	0	0	0	2	0
...........................TGTCGACGAGCCATTCCGG..........................................................................................................	19	8	0.38	3	0	0	0	3	0
...........................TGTCGACGAGCCATTCCGGTGGTT.....................................................................................................	24	6	0.33	2	0	0	0	2	0
...........................................................CAATTGAGGGCTGCGGGG...........................................................................	18	3	0.33	1	0	1	0	0	0
....................................GCCATTCCGGTGGTGTGAGGACCCAATT........................................................................................	28	3	0.33	1	0	1	0	0	0
......................................................GGACCCAATTGAGGGCTGCGGGG...........................................................................	23	3	0.33	1	0	1	0	0	0
....................................GCCATTCCGGTGGTGTGAGGACCCAAT.........................................................................................	27	3	0.33	1	0	1	0	0	0
.................................................TGTGAGGACCCAATTGAGGGCTG................................................................................	23	4	0.25	1	0	1	0	0	0
.........................................................CCCAATTGAGGGCTGCGGG............................................................................	19	4	0.25	1	0	1	0	0	0
...................................................TGAGGACCCAATTGAGGGCTGCG..............................................................................	23	4	0.25	1	0	1	0	0	0
.................................................TGTGAGGACCCAATTGAGGGCTGCGGT............................................................................	27	4	0.25	1	0	0	0	1	0
................................................GTGTGAGGACCCAATTGAGGGCTG................................................................................	24	4	0.25	1	0	1	0	0	0
.................................CGAGCCATTCCGGTGGTGTGAGG................................................................................................	23	4	0.25	1	0	0	0	1	0
.................................................TGTGAGGACCCAATTGAGG....................................................................................	19	4	0.25	1	0	0	0	1	0
..................................................GTGAGGACCCAATTGAGGGCTGCGG.............................................................................	25	4	0.25	1	0	1	0	0	0
.............................TCGACGAGCCATTCCGGTGGTGTGAG.................................................................................................	26	4	0.25	1	0	0	0	1	0
....................................................GAGGACCCAATTGAGGGCTGCGG.............................................................................	23	4	0.25	1	0	1	0	0	0
.................................................TGTGAGGACCCAATTGAGGGC..................................................................................	21	4	0.25	1	0	1	0	0	0
.................................................TGTGAGGACCCAATTGAGGGCTGCGGG............................................................................	27	4	0.25	1	0	1	0	0	0
....................................GCCATTCCGGTGGTGTGAGGACCC............................................................................................	24	4	0.25	1	0	1	0	0	0
...............................................................................................................................TGGTCCAGGGCTAGTGTGGGTTGGC	25	4	0.25	1	0	1	0	0	0
...............................................................................................................................TGGTCCAGGGCTAGTGTGGGTT...	22	4	0.25	1	0	1	0	0	0
...................................................TGAGGACCCAATTGAGGGCT.................................................................................	20	4	0.25	1	0	1	0	0	0
..............................................................................................................................TTGGTCCAGGGCTAGTGTGGGTTGG.	25	4	0.25	1	0	1	0	0	0
....................................GCCATTCCGGTGGTGTGAGGA...............................................................................................	21	4	0.25	1	0	0	0	1	0
..................................................................................................................................TCCAGGGCTAGTGTGGGTTGG.	21	4	0.25	1	0	1	0	0	0
....................................GCCATTCCGGTGGTGTGAGGACCCA...........................................................................................	25	4	0.25	1	0	1	0	0	0
.............................TCGACGAGCCATTCCGGTGGTGTGAGG................................................................................................	27	4	0.25	1	0	1	0	0	0
................................................................................................................................GGTCCAGGGCTAGTGTGGGTTGGC	24	4	0.25	1	0	1	0	0	0
..............................................................................................................................TTGGTCCAGGGCTAGTGTGGGTT...	23	4	0.25	1	0	0	0	1	0
..................................GAGCCATTCCGGTGGTGTGAGGAC..............................................................................................	24	4	0.25	1	0	1	0	0	0
..............................................................................................................................TTGGTCCAGGGCTAGTGTGGGTTAAG	26	4	0.25	1	0	0	0	1	0
....................................GCCATTCCGGTGGTGTGAGGACCCAA..........................................................................................	26	4	0.25	1	0	1	0	0	0
...................................AGCCATTCCGGTGGTGTGAGGACC.............................................................................................	24	4	0.25	1	0	1	0	0	0
.................................................TGTGAGGACCCAATTGAGGGCTGCT..............................................................................	25	4	0.25	1	0	1	0	0	0
...............................................GGTGTGAGGACCCAATTGAGGGC..................................................................................	23	4	0.25	1	0	0	0	1	0
..............................CGACGAGCCATTCCGGTGGTGTGAG.................................................................................................	25	4	0.25	1	0	1	0	0	0
...............................GACGAGCCATTCCGGTGGTG.....................................................................................................	20	5	0.20	1	0	0	0	1	0
..............................CGACGAGCCATTCCGGTGGTG.....................................................................................................	21	5	0.20	1	0	1	0	0	0
..............................CGACGAGCCATTCCGGTGGTGTGA..................................................................................................	24	5	0.20	1	0	1	0	0	0
...........................TGTCGACGAGCCATTCCGGTGGTGTATA.................................................................................................	28	5	0.20	1	0	0	1	0	0
...........................TGTCGACGAGCCATTCCGGTGGTGA....................................................................................................	25	5	0.20	1	0	0	0	1	0
...........................TGTCGACGAGCCATTCCGGTGGTGTTC..................................................................................................	27	5	0.20	1	0	1	0	0	0
...........................TGTCGACGAGCCATTCCGGTGGTGG....................................................................................................	25	6	0.17	1	0	0	0	1	0
..........................GTGTCGACGAGCCATTCCGGTGG.......................................................................................................	23	6	0.17	1	0	1	0	0	0
.............................TCGACGAGCCATTCCGGTGC.......................................................................................................	20	7	0.14	1	0	1	0	0	0
...................................................................................................TAGATCGGCGCAAAGGGCCTCG...............................	22	18	0.06	1	0	1	0	0	0

dwi_4132

scf2_1100000004969:264927-264978 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-12.8	-12.4	-12.2