dwi_4094

##################################################--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- TTGATTCGACACAGGAGTATGTCAACGAAATGATTATGACTGCCATTAAGGTAAACAATGCTGGGCTTTGCATAAGTGACGAGGTAACGGCATCACTAATGCTAAGAGGGACAAAGCAAAGTTAAATAAGCGTTTTGGGAAAACAGTTATTTGCTACACATGCGGTGGTAAAGTGCATAAGGGAGTATGTTCCGAATTTGTGCGGAAATCTGTTATCTGTAAGCCAAATGGCAAAAAAAGGGAAAAGGCTT ************************************************.......((((((.((((((((....)).))))))..))))))....((((((......(((........))).....))))))...........((..((((((((.......))))))))..))....((((.....))))........************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M045 female body	V118 embryo	M020 head	V119 head
...............................................AAGGTAAACAATGCTGGGCTTTGC....................................................................................................................................................................................	24	0	10	1.00	10	10	0	0	0
...............................................AAGGTAAACAATGCTGGGCTTTG.....................................................................................................................................................................................	23	0	10	0.40	4	4	0	0	0
................................................AGGTAAACAATGCTGGGCTTTGCA...................................................................................................................................................................................	24	0	11	0.36	4	3	0	0	1
...................TGTCAACGAAATGATTATGACTGC................................................................................................................................................................................................................	24	0	7	0.29	2	2	0	0	0
...............................................AAGGTAAACAATGCTGGGCTTTGCA...................................................................................................................................................................................	25	0	10	0.20	2	2	0	0	0
...................TGTCAACGAAATGATTATGACTGCCA..............................................................................................................................................................................................................	26	0	7	0.14	1	1	0	0	0
.........CACAGGAGTATGTCAACGAAAT............................................................................................................................................................................................................................	22	0	7	0.14	1	1	0	0	0
.....................TCAACGAAATGATTATGACTGCCAT.............................................................................................................................................................................................................	25	0	8	0.13	1	1	0	0	0
.............................ATGATTATGACTGCCATTAAGGTAAA....................................................................................................................................................................................................	26	0	9	0.11	1	1	0	0	0
.....................................................................................................................................................................................................TTGTGCGGAAATCTGTTATCTGTAAGC...........................	27	0	9	0.11	1	0	0	1	0
.......................AACGAAATGATTATGACTGCCATTAA..........................................................................................................................................................................................................	26	0	10	0.10	1	1	0	0	0
...............................................AAGGTAAACAATGCTGGGCTTTGCAT..................................................................................................................................................................................	26	0	10	0.10	1	1	0	0	0
...................................................TAAACAATGCTGGGCTTTGCATAAGT..............................................................................................................................................................................	26	0	10	0.10	1	0	1	0	0
.............................................................................................................................................................................................................AAATCTGTTATCTGTAAGCCAAATGG....................	26	0	10	0.10	1	1	0	0	0
..........................GAAATGATTATGACTGCCATTAAGG........................................................................................................................................................................................................	25	0	10	0.10	1	1	0	0	0
...............................................................................................................................................................................................................ATCTGTTATCTGTAAGCCAAATGG....................	24	0	10	0.10	1	1	0	0	0
................................................AGGTAAACAATGCTGGGCTTTGC....................................................................................................................................................................................	23	0	11	0.09	1	1	0	0	0
................................................AGGTAAACAATGCTGGGATTTGCA...................................................................................................................................................................................	24	1	11	0.09	1	0	1	0	0
.............................................TTATGTTAAAAAATGCTGGG..........................................................................................................................................................................................	20	3	20	0.05	1	1	0	0	0

##################################################---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
TTGATTCGACACAGGAGTATGTCAACGAAATGATTATGACTGCCATTAAGGTAAACAATGCTGGGCTTTGCATAAGTGACGAGGTAACGGCATCACTAATGCTAAGAGGGACAAAGCAAAGTTAAATAAGCGTTTTGGGAAAACAGTTATTTGCTACACATGCGGTGGTAAAGTGCATAAGGGAGTATGTTCCGAATTTGTGCGGAAATCTGTTATCTGTAAGCCAAATGGCAAAAAAAGGGAAAAGGCTT
**************************************************.......((((((.((((((((....)).))))))..))))))....((((((......(((........))).....))))))...........((..((((((((.......))))))))..))....((((.....))))........**************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M045
female body

V118
embryo

M020
head

V119
head

...............................................AAGGTAAACAATGCTGGGCTTTGC....................................................................................................................................................................................

1.00

...............................................AAGGTAAACAATGCTGGGCTTTG.....................................................................................................................................................................................

0.40

................................................AGGTAAACAATGCTGGGCTTTGCA...................................................................................................................................................................................

0.36

...................TGTCAACGAAATGATTATGACTGC................................................................................................................................................................................................................

0.29

...............................................AAGGTAAACAATGCTGGGCTTTGCA...................................................................................................................................................................................

0.20

...................TGTCAACGAAATGATTATGACTGCCA..............................................................................................................................................................................................................

0.14

.........CACAGGAGTATGTCAACGAAAT............................................................................................................................................................................................................................

0.14

.....................TCAACGAAATGATTATGACTGCCAT.............................................................................................................................................................................................................

0.13

.............................ATGATTATGACTGCCATTAAGGTAAA....................................................................................................................................................................................................

0.11

.....................................................................................................................................................................................................TTGTGCGGAAATCTGTTATCTGTAAGC...........................

0.11

.......................AACGAAATGATTATGACTGCCATTAA..........................................................................................................................................................................................................

0.10

...............................................AAGGTAAACAATGCTGGGCTTTGCAT..................................................................................................................................................................................

0.10

...................................................TAAACAATGCTGGGCTTTGCATAAGT..............................................................................................................................................................................

0.10

.............................................................................................................................................................................................................AAATCTGTTATCTGTAAGCCAAATGG....................

0.10

..........................GAAATGATTATGACTGCCATTAAGG........................................................................................................................................................................................................

0.10

...............................................................................................................................................................................................................ATCTGTTATCTGTAAGCCAAATGG....................

0.10

................................................AGGTAAACAATGCTGGGCTTTGC....................................................................................................................................................................................

0.09

................................................AGGTAAACAATGCTGGGATTTGCA...................................................................................................................................................................................

0.09

.............................................TTATGTTAAAAAATGCTGGG..........................................................................................................................................................................................

0.05

AACTAAGCTGTGTCCTCATACAGTTGCTTTACTAATACTGACGGTAATTCCATTTGTTACGACCCGAAACGTATTCACTGCTCCATTGCCGTAGTGATTACGATTCTCCCTGTTTCGTTTCAATTTATTCGCAAAACCCTTTTGTCAATAAACGATGTGTACGCCACCATTTCACGTATTCCCTCATACAAGGCTTAAACACGCCTTTAGACAATAGACATTCGGTTTACCGTTTTTTTCCCTTTTCCGAA ************************************************.......((((((.((((((((....)).))))))..))))))....((((((......(((........))).....))))))...........((..((((((((.......))))))))..))....((((.....))))........************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M045 female body	V118 embryo
...........GTCCTCATACAGTTGCTTTACTAAT.......................................................................................................................................................................................................................	25	0	7	1.57	11	10	1
........TGTGTCCTCATACAGTTGCTTTACT..........................................................................................................................................................................................................................	25	0	7	1.00	7	7	0
.........................................................................TTCACTGCTCCATTGCCGTAGTGAT.........................................................................................................................................................	25	0	2	1.00	2	2	0
...................................................................................................................................................................................................TAAACACGCCTTTAGACAATAGACATT.............................	27	0	9	0.56	5	5	0
...............................................................CCGAAACGTATTCACTGCTCCATTGCCGT...............................................................................................................................................................	29	0	8	0.50	4	4	0
..............................ACTAATACTGACGGTAATTCCATTTGT..................................................................................................................................................................................................	27	0	9	0.44	4	1	3
.............................TACTAATACTGACGGTAATTCCATTTGT..................................................................................................................................................................................................	28	0	9	0.44	4	4	0
.........GTGTCCTCATACAGTTGCTTTACTAAT.......................................................................................................................................................................................................................	27	0	7	0.43	3	3	0
.........................................................TACGACCCGAAACGTATTCACTGCTCCAT.....................................................................................................................................................................	29	0	8	0.38	3	3	0
...................................................................................................................................................................................................TAAACACGCCTTTAGACAATAGACAT..............................	26	0	9	0.33	3	3	0
...........GTCCTCATACAGTTGCTTTACTAATACT....................................................................................................................................................................................................................	28	0	7	0.29	2	2	0
...........................................................................................................................................................................................ACAAGGCTTAAACACGCCTTTAGACAAT....................................	28	0	8	0.25	2	2	0
.........................................................TACGACCCGAAACGTATTCACTGCT.........................................................................................................................................................................	25	0	8	0.25	2	1	1
.............CCTCATACAGTTGCTTTACTAATACT....................................................................................................................................................................................................................	26	0	8	0.25	2	2	0
............................................................GACCCGAAACGTATTCACTGCTCCAT.....................................................................................................................................................................	26	0	8	0.25	2	2	0
..................................................................AAACGTATTCACTGCTCCATTGCCGT...............................................................................................................................................................	26	0	9	0.22	2	2	0
..............................................................................................................................................................................................................TTAGACAATAGACATTCGGTTTACCGT..................	27	0	9	0.22	2	2	0
....................................................................................................................................................................................................AAACACGCCTTTAGACAATAGACATT.............................	26	0	9	0.22	2	1	1
....................................................................ACGTATTCACTGCTCCATTGCCGT...............................................................................................................................................................	24	0	9	0.22	2	1	1
.....................................................................................................................................................................................................AACACGCCTTTAGACAATAGACATT.............................	25	0	9	0.22	2	1	1
......................................................................................................................................................................................................ACACGCCTTTAGACAATAGACATT.............................	24	0	9	0.22	2	1	1
.........................................................................................................CTCCCTGTTTCGTTTCAATTTATT..........................................................................................................................	24	0	5	0.20	1	1	0
........................................................................................................TCTCCCTGTTTCGTTTCAATTTAT...........................................................................................................................	24	0	5	0.20	1	1	0
.TAGAAGCTGTGTCCTCATACAGTTGCT...............................................................................................................................................................................................................................	27	3	6	0.17	1	1	0
...TAAGCTGTGTCCTCATACAGTTGCTT..............................................................................................................................................................................................................................	26	0	6	0.17	1	1	0
..CTAAGCTGTGTCCTCATACAGT...................................................................................................................................................................................................................................	22	0	6	0.17	1	1	0
.....AGCTGTGTCCTCATACAGTTGCTTT.............................................................................................................................................................................................................................	25	0	6	0.17	1	1	0
....AAGCTGTGTCCTCATACAGTTGCT...............................................................................................................................................................................................................................	24	0	6	0.17	1	1	0
...TAAGCTGTGTCCTCATACAGTTGCT...............................................................................................................................................................................................................................	25	0	6	0.17	1	1	0
.........GTGTCCTCATACAGTTGCTTTACT..........................................................................................................................................................................................................................	24	0	7	0.14	1	1	0
...........GTCCTCATACAGTTGCTTTACTAA........................................................................................................................................................................................................................	24	0	7	0.14	1	1	0
....................................................................ACGTATTCACTGCTCCATTGCCGTAGT............................................................................................................................................................	27	0	7	0.14	1	1	0
........TGTGTCCTCATACAGTTGC................................................................................................................................................................................................................................	19	0	7	0.14	1	1	0
...........GTCCCCATACAGTTGCTTTACTAATACT....................................................................................................................................................................................................................	28	1	7	0.14	1	1	0
..........TGTCCTCATACAGTTGCTTTACTAAT.......................................................................................................................................................................................................................	26	0	7	0.14	1	1	0
.........GTGTCCTCATACAGTTGCTTTACTAA........................................................................................................................................................................................................................	26	0	7	0.14	1	1	0
.....................................................................CGTATTCACTGCTCCATTGCCGTAGT............................................................................................................................................................	26	0	7	0.14	1	0	1
.......................................................GTTACGACCCGAAACGTATTCACTGCT.........................................................................................................................................................................	27	0	8	0.13	1	1	0
............................................................GACCCGAAACGTATTCACTGCTCCATT....................................................................................................................................................................	27	0	8	0.13	1	1	0
...............................................................................................................................................................................................................TAGACAATAGACATTCGGTTTACCGTTT................	28	0	8	0.13	1	1	0
............................................................................................................................................................................................CAAGGCTTAAACACGCCTTTAGACAAT....................................	27	0	8	0.13	1	1	0
...............................................................................................................................................................................................GGCTTAAACACGCCTTTAGACAA.....................................	23	0	8	0.13	1	1	0
.............CCTCATACAGTTGCTTTACTAATAC.....................................................................................................................................................................................................................	25	0	8	0.13	1	1	0
...........................................................................................................................................................................................ACAAGGCTTAAACACGCCTTTAGACAA.....................................	27	0	8	0.13	1	0	1
...........................................................CGACCCGAAACGTATTCACTGCTCCA......................................................................................................................................................................	26	0	8	0.13	1	1	0
..........................................................ACGACCCGAAACGTATTCACTGCTCCAT.....................................................................................................................................................................	28	0	8	0.13	1	1	0
.......................................................................................................................................................................................................CACGCCTTTAGACAATAGACATTCGGT.........................	27	0	8	0.13	1	1	0
...............................................................................................................................................................................................................TAGACAATAGACATTCGGTTTACCGTT.................	27	0	8	0.13	1	1	0
............................................................TACCCGAAACGTATTCACTGCTCCATT....................................................................................................................................................................	27	1	8	0.13	1	1	0
..........................................................ACGACCCGAAACGTATTCACTGCTCCATT....................................................................................................................................................................	29	0	8	0.13	1	1	0
........................................................................................................................................................................................................ACGCCTTTAGACAATAGACATTCGGT.........................	26	0	8	0.13	1	1	0
..............................................................................................................................................................................................AGGCTTAAACACGCCTTTAGACAAT....................................	25	0	8	0.13	1	1	0
.....................................................................................................................................................................................................AACACGCCTTTAGACAATAGACATTCGGT.........................	29	0	8	0.13	1	1	0
...............................................................................................................................................................................................GGCTTAAACACGCCTTTAGACAAT....................................	24	0	8	0.13	1	0	1
............................TTACTAATACTGACGGTAATTCCATTTG...................................................................................................................................................................................................	28	0	9	0.11	1	1	0
..............................................ATTCCATTTGTTACGACCCGAAACGT...................................................................................................................................................................................	26	0	9	0.11	1	1	0
............................TTACTAATACTGACGGTAATTCCAT......................................................................................................................................................................................................	25	0	9	0.11	1	1	0
..............................................................................................................................................................................................................TTAGACAATAGACATTCGGTTTACCG...................	26	0	9	0.11	1	1	0
............................TTACTAATACTGACGGTAATTCCATTTGT..................................................................................................................................................................................................	29	0	9	0.11	1	1	0
........................TGCTTTACTAATACTGACGGTAATTCCAT......................................................................................................................................................................................................	29	0	9	0.11	1	1	0
.................................AATACTGACGGTAATTCCATTTGTT.................................................................................................................................................................................................	25	0	9	0.11	1	1	0
........................................................................ATTCACTGCTCCATTGCCGT...............................................................................................................................................................	20	0	9	0.11	1	0	1
...........................................................CGACCCGAAACGTATTCACT............................................................................................................................................................................	20	0	9	0.11	1	0	1
................................................................CGAAACGTATTCACTGCTCCATTGCCGT...............................................................................................................................................................	28	0	9	0.11	1	1	0
.................................AATACGGACGGTAATTCCATTTGT..................................................................................................................................................................................................	24	1	9	0.11	1	1	0
...................................................................AACGTATTCACTGCTCCATTGCCGT...............................................................................................................................................................	25	0	9	0.11	1	1	0
.......................................................GTTACGACCCGAAACGTATTCACTGC..........................................................................................................................................................................	26	0	9	0.11	1	1	0
.................................AATACTGACGGTAATTCCATTTGT..................................................................................................................................................................................................	24	0	9	0.11	1	1	0
...............................................TTCCATTTGTTACGACCCGAAACGT...................................................................................................................................................................................	25	0	10	0.10	1	1	0
.....................................................................................................................................AAACCCTTTTGTCAATAAACGAT...............................................................................................	23	0	12	0.08	1	1	0

AACTAAGCTGTGTCCTCATACAGTTGCTTTACTAATACTGACGGTAATTCCATTTGTTACGACCCGAAACGTATTCACTGCTCCATTGCCGTAGTGATTACGATTCTCCCTGTTTCGTTTCAATTTATTCGCAAAACCCTTTTGTCAATAAACGATGTGTACGCCACCATTTCACGTATTCCCTCATACAAGGCTTAAACACGCCTTTAGACAATAGACATTCGGTTTACCGTTTTTTTCCCTTTTCCGAA
**************************************************.......((((((.((((((((....)).))))))..))))))....((((((......(((........))).....))))))...........((..((((((((.......))))))))..))....((((.....))))........**************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M045
female body

V118
embryo

...........GTCCTCATACAGTTGCTTTACTAAT.......................................................................................................................................................................................................................

1.57

........TGTGTCCTCATACAGTTGCTTTACT..........................................................................................................................................................................................................................

1.00

.........................................................................TTCACTGCTCCATTGCCGTAGTGAT.........................................................................................................................................................

1.00

...................................................................................................................................................................................................TAAACACGCCTTTAGACAATAGACATT.............................

0.56

...............................................................CCGAAACGTATTCACTGCTCCATTGCCGT...............................................................................................................................................................

0.50

..............................ACTAATACTGACGGTAATTCCATTTGT..................................................................................................................................................................................................

0.44

.............................TACTAATACTGACGGTAATTCCATTTGT..................................................................................................................................................................................................

0.44

.........GTGTCCTCATACAGTTGCTTTACTAAT.......................................................................................................................................................................................................................

0.43

.........................................................TACGACCCGAAACGTATTCACTGCTCCAT.....................................................................................................................................................................

0.38

...................................................................................................................................................................................................TAAACACGCCTTTAGACAATAGACAT..............................

0.33

...........GTCCTCATACAGTTGCTTTACTAATACT....................................................................................................................................................................................................................

0.29

...........................................................................................................................................................................................ACAAGGCTTAAACACGCCTTTAGACAAT....................................

0.25

.........................................................TACGACCCGAAACGTATTCACTGCT.........................................................................................................................................................................

0.25

.............CCTCATACAGTTGCTTTACTAATACT....................................................................................................................................................................................................................

0.25

............................................................GACCCGAAACGTATTCACTGCTCCAT.....................................................................................................................................................................

0.25

..................................................................AAACGTATTCACTGCTCCATTGCCGT...............................................................................................................................................................

0.22

..............................................................................................................................................................................................................TTAGACAATAGACATTCGGTTTACCGT..................

0.22

....................................................................................................................................................................................................AAACACGCCTTTAGACAATAGACATT.............................

0.22

....................................................................ACGTATTCACTGCTCCATTGCCGT...............................................................................................................................................................

0.22

.....................................................................................................................................................................................................AACACGCCTTTAGACAATAGACATT.............................

0.22

......................................................................................................................................................................................................ACACGCCTTTAGACAATAGACATT.............................

0.22

.........................................................................................................CTCCCTGTTTCGTTTCAATTTATT..........................................................................................................................

0.20

........................................................................................................TCTCCCTGTTTCGTTTCAATTTAT...........................................................................................................................

0.20

.TAGAAGCTGTGTCCTCATACAGTTGCT...............................................................................................................................................................................................................................

0.17

...TAAGCTGTGTCCTCATACAGTTGCTT..............................................................................................................................................................................................................................

0.17

..CTAAGCTGTGTCCTCATACAGT...................................................................................................................................................................................................................................

0.17

.....AGCTGTGTCCTCATACAGTTGCTTT.............................................................................................................................................................................................................................

0.17

....AAGCTGTGTCCTCATACAGTTGCT...............................................................................................................................................................................................................................

0.17

...TAAGCTGTGTCCTCATACAGTTGCT...............................................................................................................................................................................................................................

0.17

.........GTGTCCTCATACAGTTGCTTTACT..........................................................................................................................................................................................................................

0.14

...........GTCCTCATACAGTTGCTTTACTAA........................................................................................................................................................................................................................

0.14

....................................................................ACGTATTCACTGCTCCATTGCCGTAGT............................................................................................................................................................

0.14

........TGTGTCCTCATACAGTTGC................................................................................................................................................................................................................................

0.14

...........GTCCCCATACAGTTGCTTTACTAATACT....................................................................................................................................................................................................................

0.14

..........TGTCCTCATACAGTTGCTTTACTAAT.......................................................................................................................................................................................................................

0.14

.........GTGTCCTCATACAGTTGCTTTACTAA........................................................................................................................................................................................................................

0.14

.....................................................................CGTATTCACTGCTCCATTGCCGTAGT............................................................................................................................................................

0.14

.......................................................GTTACGACCCGAAACGTATTCACTGCT.........................................................................................................................................................................

0.13

............................................................GACCCGAAACGTATTCACTGCTCCATT....................................................................................................................................................................

0.13

...............................................................................................................................................................................................................TAGACAATAGACATTCGGTTTACCGTTT................

0.13

............................................................................................................................................................................................CAAGGCTTAAACACGCCTTTAGACAAT....................................

0.13

...............................................................................................................................................................................................GGCTTAAACACGCCTTTAGACAA.....................................

0.13

.............CCTCATACAGTTGCTTTACTAATAC.....................................................................................................................................................................................................................

0.13

...........................................................................................................................................................................................ACAAGGCTTAAACACGCCTTTAGACAA.....................................

0.13

...........................................................CGACCCGAAACGTATTCACTGCTCCA......................................................................................................................................................................

0.13

..........................................................ACGACCCGAAACGTATTCACTGCTCCAT.....................................................................................................................................................................

0.13

.......................................................................................................................................................................................................CACGCCTTTAGACAATAGACATTCGGT.........................

0.13

...............................................................................................................................................................................................................TAGACAATAGACATTCGGTTTACCGTT.................

0.13

............................................................TACCCGAAACGTATTCACTGCTCCATT....................................................................................................................................................................

0.13

..........................................................ACGACCCGAAACGTATTCACTGCTCCATT....................................................................................................................................................................

0.13

........................................................................................................................................................................................................ACGCCTTTAGACAATAGACATTCGGT.........................

0.13

..............................................................................................................................................................................................AGGCTTAAACACGCCTTTAGACAAT....................................

0.13

.....................................................................................................................................................................................................AACACGCCTTTAGACAATAGACATTCGGT.........................

0.13

...............................................................................................................................................................................................GGCTTAAACACGCCTTTAGACAAT....................................

0.13

............................TTACTAATACTGACGGTAATTCCATTTG...................................................................................................................................................................................................

0.11

..............................................ATTCCATTTGTTACGACCCGAAACGT...................................................................................................................................................................................

0.11

............................TTACTAATACTGACGGTAATTCCAT......................................................................................................................................................................................................

0.11

..............................................................................................................................................................................................................TTAGACAATAGACATTCGGTTTACCG...................

0.11

............................TTACTAATACTGACGGTAATTCCATTTGT..................................................................................................................................................................................................

0.11

........................TGCTTTACTAATACTGACGGTAATTCCAT......................................................................................................................................................................................................

0.11

.................................AATACTGACGGTAATTCCATTTGTT.................................................................................................................................................................................................

0.11

........................................................................ATTCACTGCTCCATTGCCGT...............................................................................................................................................................

0.11

...........................................................CGACCCGAAACGTATTCACT............................................................................................................................................................................

0.11

................................................................CGAAACGTATTCACTGCTCCATTGCCGT...............................................................................................................................................................

0.11

.................................AATACGGACGGTAATTCCATTTGT..................................................................................................................................................................................................

0.11

...................................................................AACGTATTCACTGCTCCATTGCCGT...............................................................................................................................................................

0.11

.......................................................GTTACGACCCGAAACGTATTCACTGC..........................................................................................................................................................................

0.11

.................................AATACTGACGGTAATTCCATTTGT..................................................................................................................................................................................................

0.11

...............................................TTCCATTTGTTACGACCCGAAACGT...................................................................................................................................................................................

0.10

.....................................................................................................................................AAACCCTTTTGTCAATAAACGAT...............................................................................................

0.08

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droWil2	scf2_1100000004968:877871-878121 +	dwi_4094	TTGATTCGACACAGGAGTATGTCAACGAAATGATTATGACTGCCATTAAGGTAAACAATGCTGGGCTTTGCATAAGTGACGAGGTAACGGCATCACTAATGCTAAG-------------AGGGACAAAGCAAAGTTAAATAAGCGTTTTGGGAAAACAGTTATTTGCTACACATGCGGTGGTAAAGTGCATAAG-----GGAGTATGTTCCGAATTTGTGCGGAAATCTGTTATCTGTAAGCCAAATGGCAAAAAAAGGGAA------AAGGCTT
droGri2	scaffold_9515:4689-4842 +		ACGG------------------------------------------------------------------------------ATTAGTGGCATTGGTAATGCTAAAGTCGCAGTTAAGAAACAACAGCAACGAGTTGACTTAACGT-------------------------------------------TGAAAGATGTTTTCTACGTATCGGATTTGCACCAAAATTTGATGTCGGTTTCCAAAATCACAGACAATACCACAGTATTAAAGTTC
droAna3	scaffold_13337:23205230-23205315 -		AATT---------------------------------------------------------------------------------------------------------------------------------------------C-------------------------------------------TTAAAGAAGTGCTTCATGTGTCAGATTTGCGCCAAAATTTATTGTCAGTTTCAAAAATGACCGACAAAGGGTA-----CGAGGTAC
droKik1	scf7180000300586:7004-7083 -		T----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AAAGAAGTGCTATTTGTGTCGGATCTTTGCCAGAATTTGCTTTCTGTTTCAAAAATAACGGAAAAAGGTTA-----CGAAGTGC
droFic1	scf7180000448921:5609-5672 +		TGTT-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CCAAAGATGAACGGAAATTTCATGTCGGTAAGCAGTGCTGTGCAGAACAGGTG-----CAAGG-TG
droTak1	scf7180000415029:2225-2284 -		GCGGCAATATACGAGAGCACATAAACGAA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ACATTTGAAGCCGTTAA-GAAATTAAGG--------------------GAAA

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droWil2

TTGATTCGACACAGGAGTATGTCAACGAAATGATTATGACTGCCATTAAGGTAAACAATGCTGGGCTTTGCATAAGTGACGAGGTAACGGCATCACTAATGCTAAG-------------AGGGACAAAGCAAAGTTAAATAAGCGTTTTGGGAAAACAGTTATTTGCTACACATGCGGTGGTAAAGTGCATAAG-----GGAGTATGTTCCGAATTTGTGCGGAAATCTGTTATCTGTAAGCCAAATGGCAAAAAAAGGGAA------AAGGCTT	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
...............................................AAGGTAAACAATGCTGGGCTTTGC............................................................................................................................................................................................................	24	10	1.00	10	0	10	0	0	0
...............................................AAGGTAAACAATGCTGGGCTTTG.............................................................................................................................................................................................................	23	10	0.40	4	0	4	0	0	0
................................................AGGTAAACAATGCTGGGCTTTGCA...........................................................................................................................................................................................................	24	11	0.36	4	0	3	0	0	1
...................TGTCAACGAAATGATTATGACTGC........................................................................................................................................................................................................................................	24	7	0.29	2	0	2	0	0	0
...............................................AAGGTAAACAATGCTGGGCTTTGCA...........................................................................................................................................................................................................	25	10	0.20	2	0	2	0	0	0
...................TGTCAACGAAATGATTATGACTGCCA......................................................................................................................................................................................................................................	26	7	0.14	1	0	1	0	0	0
.........CACAGGAGTATGTCAACGAAAT....................................................................................................................................................................................................................................................	22	7	0.14	1	0	1	0	0	0
.....................TCAACGAAATGATTATGACTGCCAT.....................................................................................................................................................................................................................................	25	8	0.13	1	0	1	0	0	0
.............................ATGATTATGACTGCCATTAAGGTAAA............................................................................................................................................................................................................................	26	9	0.11	1	0	1	0	0	0
.......................................................................................................................................................................................................................TTGTGCGGAAATCTGTTATCTGTAAGC.................................	27	9	0.11	1	1	0	0	0	0
.......................AACGAAATGATTATGACTGCCATTAA..................................................................................................................................................................................................................................	26	10	0.10	1	0	1	0	0	0
...............................................AAGGTAAACAATGCTGGGCTTTGCAT..........................................................................................................................................................................................................	26	10	0.10	1	0	1	0	0	0
...................................................TAAACAATGCTGGGCTTTGCATAAGT......................................................................................................................................................................................................	26	10	0.10	1	0	0	0	1	0
...............................................................................................................................................................................................................................AAATCTGTTATCTGTAAGCCAAATGG..........................	26	10	0.10	1	0	1	0	0	0
..........................GAAATGATTATGACTGCCATTAAGG................................................................................................................................................................................................................................	25	10	0.10	1	0	1	0	0	0
.................................................................................................................................................................................................................................ATCTGTTATCTGTAAGCCAAATGG..........................	24	10	0.10	1	0	1	0	0	0
................................................AGGTAAACAATGCTGGGCTTTGC............................................................................................................................................................................................................	23	11	0.09	1	0	1	0	0	0

droGri2

ACGG------------------------------------------------------------------------------ATTAGTGGCATTGGTAATGCTAAAGTCGCAGTTAAGAAACAACAGCAACGAGTTGACTTAACGT-------------------------------------------TGAAAGATGTTTTCTACGTATCGGATTTGCACCAAAATTTGATGTCGGTTTCCAAAATCACAGACAATACCACAGTATTAAAGTTC	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

droAna3

AATT---------------------------------------------------------------------------------------------------------------------------------------------C-------------------------------------------TTAAAGAAGTGCTTCATGTGTCAGATTTGCGCCAAAATTTATTGTCAGTTTCAAAAATGACCGACAAAGGGTA-----CGAGGTAC	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
..........................................................................................................................................................................................................................................CAGTTTCAAAAATGACCGACAAAGGG...............	26	9	0.11	1	1	0	0	0	0	0
.................................................................................................................................................C-------------------------------------------TTAAAGAAGTGCTTCATGTGTC................................................................	23	12	0.08	1	0	1	0	0	0	0
..........................................................................................................................................................................................................................................CAGTTTCAAAAATGACCGAC.....................	20	13	0.08	1	0	1	0	0	0	0

droKik1

T----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AAAGAAGTGCTATTTGTGTCGGATCTTTGCCAGAATTTGCTTTCTGTTTCAAAAATAACGGAAAAAGGTTA-----CGAAGTGC	Size	Hit Count	Total Norm	Total
No Reads

droFic1

TGTT-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CCAAAGATGAACGGAAATTTCATGTCGGTAAGCAGTGCTGTGCAGAACAGGTG-----CAAGG-TG	Size	Hit Count	Total Norm	Total
No Reads

droTak1

GCGGCAATATACGAGAGCACATAAACGAA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ACATTTGAAGCCGTTAA-GAAATTAAGG--------------------GAAA	Size	Hit Count	Total Norm	Total
No Reads

dwi_4094

scf2_1100000004968:877921-878071 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-28.8	-28.8	-28.7