ID:

dwi_3866

 Coordinate:

scf2_1100000004961:1021169-1021319 +

 Confidence:

Candidate

 Type:

Unknown

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

No conservation details.

-14.4 -14.4 -13.4

Flybase annnotation

CDS [Dwil\GK23429-cds]; exon [dwil_GLEANR_6886:3]; intron [Dwil\GK23429-in]

Repeatable elements

NameClassFamilyStrand
Gypsy-12_DWil-I-intLTRGypsy+

Sense Strand Reads

##################################################---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
GCTCGTCGACCTGATCATCGACGGTCTGCGGGACAACTCTACTAGAGTCAGTATGTTGTACTGAGTTCGTACGGTGGCTGAGCTGAAGCCCCTCCTGGAACGCTACGAGCGCTCCCGTGTACAGGCTGCGCCGCCAAGCAAAGGGTCAGTGTAAAGGTGCAGCCAACACCAACTGCCTTGTCGTCCGTGAGCAAATCTTCAGGCCCTGCCACGAGTGAGACCCGATGTTTCAAATGCTCGGGATGGCGGCA
**********************************************************......(((((((..((((((.......)))).........))..)))))))...****************************************************************************************************************************************** 		Read size # Mismatch Hit Count Total Norm Total M045
female body
 V117
male body		 V118
embryo
 V119
head
..........................................................TACTGAGTTCGTACGGTGGCTGAGCT....................................................................................................................................................................... 26 0 1 2.00 2 1 1 0 0
..........................................................TACTGAGTTCGTACGGTGGCTGAGC........................................................................................................................................................................ 25 0 1 1.00 1 1 0 0 0
.......................................................................................................................................................................................TCCGTGAGCAAATCTTCAGGC............................................... 21 0 1 1.00 1 1 0 0 0
............................................................................................TCCTGGAACGCTACGAGCGCT.......................................................................................................................................... 21 0 1 1.00 1 0 1 0 0
..........................................................................................CCTCCTGGAACGCTACGAGCGCTCCCG...................................................................................................................................... 27 0 1 1.00 1 1 0 0 0
......................................................................................................................................CAAGCAAAGGGTCAGTGTAAAGGTGC........................................................................................... 26 0 1 1.00 1 0 0 1 0
.........................CTGCGGGACAACTCTACTAGAGTCAGTA...................................................................................................................................................................................................... 28 0 1 1.00 1 1 0 0 0
.....................................................................................................................................................................................................................AGTGAGACCCGATGTTTCAAATGCTCG........... 27 0 1 1.00 1 1 0 0 0
..............................................................................................................................................................................................................................CGATGTTTCAAATGCTCGGGATGG..... 24 0 1 1.00 1 1 0 0 0
.............ATCATCGACCGTCTGCGT............................................................................................................................................................................................................................ 18 2 2 0.50 1 1 0 0 0
...............................................................................................................................................................................................CAAATCTTCAGGCCCTGCCACGA..................................... 23 0 5 0.40 2 2 0 0 0
..................................................................................................................................................................CCAACACCAACTGCCTTGTCGTC.................................................................. 23 0 4 0.25 1 1 0 0 0
...CGTCGACCTGATCACCTCCG.................................................................................................................................................................................................................................... 20 3 4 0.25 1 0 1 0 0
..................................................................................................AACGCTACGAGCGCTCCCGTGTACA................................................................................................................................ 25 0 5 0.20 1 1 0 0 0
...............................................................................................................................................................................................CAAATCTTCAGGCCCTGCCACGAGTGA................................. 27 0 5 0.20 1 1 0 0 0
...............................................................................................................................................................................................CAAACATTCCGGCCCTGCCACG...................................... 22 3 6 0.17 1 1 0 0 0
...............................................................................................................................GTGCGGCCTAGCAAAGGGT......................................................................................................... 19 3 11 0.09 1 0 0 0 1
.CTCGTCGGCAAGATCATCGA...................................................................................................................................................................................................................................... 20 3 15 0.07 1 0 0 0 1
........................................................................................................................................................AAAGGAGCGGCCCACACCA................................................................................ 19 3 20 0.05 1 0 0 1 0

Anti-sense strand reads

CGAGCAGCTGGACTAGTAGCTGCCAGACGCCCTGTTGAGATGATCTCAGTCATACAACATGACTCAAGCATGCCACCGACTCGACTTCGGGGAGGACCTTGCGATGCTCGCGAGGGCACATGTCCGACGCGGCGGTTCGTTTCCCAGTCACATTTCCACGTCGGTTGTGGTTGACGGAACAGCAGGCACTCGTTTAGAAGTCCGGGACGGTGCTCACTCTGGGCTACAAAGTTTACGAGCCCTACCGCCGT
******************************************************************************************************************************************......(((((((..((((((.......)))).........))..)))))))...********************************************************** 		Read size # Mismatch Hit Count Total Norm Total M045
female body
 V118
embryo
 V117
male body		 M020
head
 V119
head
........................................................................................................................................................................GGTTGACGGAACAGCAGGCACTCGT.......................................................... 25 0 1 2.00 2 2 0 0 0 0
.........................................................................................................................................CGTTTCCCAGTCACATTTCCACGTCGGT...................................................................................... 28 0 1 1.00 1 1 0 0 0 0
....................................................................................................................................................................................................GAAGTCCGGGACGGTGCTCACTCT............................... 24 0 6 1.00 6 6 0 0 0 0
...................................................................................................................GCACATGTCCGACGCGGCGGTTCGT............................................................................................................... 25 0 1 1.00 1 1 0 0 0 0
...................................................ATACAACATGACTCAAGCATGCCAC............................................................................................................................................................................... 25 0 1 1.00 1 1 0 0 0 0
....................TGCCAGACGCCCTGTTGAGATGATCT............................................................................................................................................................................................................. 26 0 1 1.00 1 1 0 0 0 0
...................................................................................................................................................................ATTGTGGTTGACTGAACAGCAGGCACT............................................................. 27 2 1 1.00 1 1 0 0 0 0
.......................................................................GCCACCGACTCGACTTCGGGGAGGACCT........................................................................................................................................................ 28 0 1 1.00 1 0 0 1 0 0
...................................TGAGATGATCTCAGTTAAA..................................................................................................................................................................................................... 19 2 6 0.50 3 0 1 0 0 2
............................................................................................................................................................CACGTCGGTTGTGGTTGACGGAACAG..................................................................... 26 0 3 0.33 1 1 0 0 0 0
..............................................................................................................................................................CGTCGGTTGTGGTTGACGGAAC....................................................................... 22 0 6 0.33 2 0 0 0 2 0
.......................CAGACGCCCTGTTGAGATG................................................................................................................................................................................................................. 19 0 3 0.33 1 0 1 0 0 0
.....................................................................................................................................................................TGTGGTTGACGGAACAGCAG.................................................................. 20 0 4 0.25 1 0 1 0 0 0
.................................................................................................................................................................................................TTGGAAGTCCAGGACGGT........................................ 18 2 8 0.25 2 2 0 0 0 0
..................................................................................................................................................................................................TAGAAGTCCGGGACGGTGCTCACTCT............................... 26 0 5 0.20 1 1 0 0 0 0
.................................................................................................................................................................................................TTAGAAGTCCGGGACGGTGCTCACT................................. 25 0 5 0.20 1 1 0 0 0 0
....................................................................................................................................................................................................GAAGTCCGGGACGGTGCTCACT................................. 22 0 6 0.17 1 1 0 0 0 0
...........................................................................................................................................................CCACGTCGGTTGTGGTTGACGGAAC....................................................................... 25 0 6 0.17 1 0 1 0 0 0
..........................................................................................................................................................................................................CGGGACGGTGCTCACTCTGG............................. 20 0 6 0.17 1 1 0 0 0 0
.....................................................................................................................................................................................................AAGTCCGGGACGGTGCTCACTCT............................... 23 0 6 0.17 1 1 0 0 0 0
..................................................................................................................................................................................ACAGGAGGTACTCGTTAAGA..................................................... 20 3 13 0.15 2 0 0 0 2 0
..................................................................................................................................................................GGTTGTGGTTGACGGAACA...................................................................... 19 0 7 0.14 1 0 1 0 0 0
....CAGGTGGACTAGTATCTG..................................................................................................................................................................................................................................... 18 2 8 0.13 1 0 0 0 1 0
......................................................................................................................CATGTATGGCGCGGCGGTT.................................................................................................................. 19 3 10 0.10 1 1 0 0 0 0
.................................................................................................................................................................................................TTGGAAGTCCGGGACGGTA....................................... 19 2 10 0.10 1 1 0 0 0 0
...........................................................................................................................................................................................................................TGGGCAAGAAAGTTTACGC............. 19 3 19 0.05 1 1 0 0 0 0
..........GACTGGTAGCTGACACACG.............................................................................................................................................................................................................................. 19 3 20 0.05 1 1 0 0 0 0
.................................................................................................................................................................................................TTGGAAGTCCAGGACGGTA....................................... 19 3 20 0.05 1 0 0 1 0 0

Re-alignment of all predicted orthologs

Species Coordinate ID Alignment
droWil2 scf2_1100000004961:1021119-1021369 + dwi_3866 GCTCGTCGACCTGATCATCGACGGTCTGCGGGACAACTCTACTAGAGTCAGTATGTTGTACTGAGTTCGTACGGTGGCTGAGCTGAAGCCCCTCCTGGAACGCTACGAGCGCTCCCGTGTACAGGCTGCGCCGCCAAGCAAAGGGTCAGTGTAAAGGTGCAGCCAACACCAACTGCCTTGTCGTCCGTGAGCAAATCTTCAGGCCCTGCCACGAGTGAGACCCGATGTTTCAAATGCTCGGGATGGCGGCA

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droWil2
GCTCGTCGACCTGATCATCGACGGTCTGCGGGACAACTCTACTAGAGTCAGTATGTTGTACTGAGTTCGTACGGTGGCTGAGCTGAAGCCCCTCCTGGAACGCTACGAGCGCTCCCGTGTACAGGCTGCGCCGCCAAGCAAAGGGTCAGTGTAAAGGTGCAGCCAACACCAACTGCCTTGTCGTCCGTGAGCAAATCTTCAGGCCCTGCCACGAGTGAGACCCGATGTTTCAAATGCTCGGGATGGCGGCASizeHit CountTotal NormTotalM020
Head		M045
Female-body		V117
Male-body		V118
Embryo		V119
Head
..........................................................TACTGAGTTCGTACGGTGGCTGAGCT.......................................................................................................................................................................2612.00201100
..........................................................TACTGAGTTCGTACGGTGGCTGAGC........................................................................................................................................................................2511.00101000
.......................................................................................................................................................................................TCCGTGAGCAAATCTTCAGGC...............................................2111.00101000
............................................................................................TCCTGGAACGCTACGAGCGCT..........................................................................................................................................2111.00100100
..........................................................................................CCTCCTGGAACGCTACGAGCGCTCCCG......................................................................................................................................2711.00101000
......................................................................................................................................CAAGCAAAGGGTCAGTGTAAAGGTGC...........................................................................................2611.00100010
.........................CTGCGGGACAACTCTACTAGAGTCAGTA......................................................................................................................................................................................................2811.00101000
.....................................................................................................................................................................................................................AGTGAGACCCGATGTTTCAAATGCTCG...........2711.00101000
..............................................................................................................................................................................................................................CGATGTTTCAAATGCTCGGGATGG.....2411.00101000
...............................................................................................................................................................................................CAAATCTTCAGGCCCTGCCACGA.....................................2350.40202000
..................................................................................................................................................................CCAACACCAACTGCCTTGTCGTC..................................................................2340.25101000
..................................................................................................AACGCTACGAGCGCTCCCGTGTACA................................................................................................................................2550.20101000
...............................................................................................................................................................................................CAAATCTTCAGGCCCTGCCACGAGTGA.................................2750.20101000

Generated: 05/18/2015 at 08:36 AM