dwi_355

##################################################--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CAAGGATCAAGCGAGCGTCGAGACACCGGACACCCCTCCGCGGTCCGTTTGTGAGTGTCTGGAGAGACGTCAATCTCCAGAAGTCGTGGAAGCAGCTATGCCGGACACCCCTCTGCGGCCCGTGTATGAGCCGATAAGACCCCCAAGGAAAACAAAAGTGTTCTTCTGACAACCGTCAGGGTCAGTGAGACTCAGTCTGACAACGAGGAGATGCCCCTAGACTTCTCCGGGCCCCGCCCTCGACCATCGCT *************************************************************..........(((((((.((..(((...(((....)))....)))..)))))((((.((....)).))))....(((((...(((((.((....)).))))).((((....)))))))))...))))..*********************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M045 female body	V117 male body	V118 embryo	V119 head
..........GAGAGCGTCGAGATCCCGGACAC..........................................................................................................................................................................................................................	23	3	1	2.00	2	0	0	1	1
..................................................................................GACGTTGAAGCAGCTATGC......................................................................................................................................................	19	2	1	2.00	2	1	1	0	0
...........AGAGCGTCGAGATCCCGGACACC.........................................................................................................................................................................................................................	23	3	1	1.00	1	0	1	0	0
....................................................................................CGTTGAAGCAGCTATGCG.....................................................................................................................................................	18	2	2	0.50	1	1	0	0	0
...............................................................GAGACGTCAATCTCCAGAAGTCGTGG..................................................................................................................................................................	26	0	6	0.33	2	2	0	0	0
...........................................................................................................................................................................ACCGTCAGGGTCAGTGAGACT...........................................................	21	0	3	0.33	1	1	0	0	0
........................................................................................................................................................................ACAACCGTCAGGGTCAGTGAGACTC..........................................................	25	0	3	0.33	1	1	0	0	0
....................................................................................................................................................................................................CTGACAACGAGGAGATCCCCCTAG...............................	24	1	3	0.33	1	0	0	1	0
................................................................................................................................................................TTCTTCTACCAACCGTCAG........................................................................	19	2	3	0.33	1	1	0	0	0
....................................................................................................................................................................................................CTGACAACGAGGAGATGCCCCTA................................	23	0	3	0.33	1	1	0	0	0
.................................................TGTGAGTGTCTGGAGAGACGTCA...................................................................................................................................................................................	23	0	11	0.27	3	2	1	0	0
......................................................................................................................................................................TGACAACCGTCAGGGTCAGTGAGAC............................................................	25	0	8	0.25	2	1	0	1	0
.................................................................................TGACGTTGAAGCAGCTATGC......................................................................................................................................................	20	3	9	0.22	2	2	0	0	0
............................................CCGTTTGTGAGTGTCTGGAGAGA........................................................................................................................................................................................	23	0	19	0.21	4	3	0	1	0
..................................................................................GACGTTGAAGCAGCTATGCG.....................................................................................................................................................	20	3	5	0.20	1	1	0	0	0
...........................................................................TCCAGAAGTCGTGGAAGCAGC...........................................................................................................................................................	21	0	11	0.18	2	1	0	1	0
.........................................................................................................CACCCCTCTGCGGCCCGTGTAT............................................................................................................................	22	0	6	0.17	1	1	0	0	0
..................................................................................................TGCCGGACACCCCTCTGCGGCCCGTG...............................................................................................................................	26	0	6	0.17	1	1	0	0	0
..............................................................AGAGACGTCAATCTCCAGAAGTCG.....................................................................................................................................................................	24	0	6	0.17	1	1	0	0	0
...........................................................................................................CCCCTCTGCGGCCCGTGTAT............................................................................................................................	20	0	6	0.17	1	1	0	0	0
.................................................................................................ATGCCGGACACCCCTCTGCGGCCCG.................................................................................................................................	25	0	7	0.14	1	1	0	0	0
.......................................................................AATCTCCAGAAGTCGTGGAAGCAG............................................................................................................................................................	24	0	8	0.13	1	1	0	0	0
........................................................................................................................................................................ACAACCGTCAGGGTCAGTGAGA.............................................................	22	0	8	0.13	1	1	0	0	0
........................................................................ATCTCCAGAAGTCGTGGAAGCAG............................................................................................................................................................	23	0	8	0.13	1	1	0	0	0
.................................................................................................................................................................................................................GATGCCCTTAGACTTCTG........................	18	2	8	0.13	1	1	0	0	0
........................................................................................................................................................................ACAACCGTCAGGGTCAGTGAG..............................................................	21	0	8	0.13	1	1	0	0	0
...............................................TTTGTGAGTGTCTGGAGAGACG......................................................................................................................................................................................	22	0	19	0.11	2	1	0	1	0
............................................CCGTTTGTGAGTGTCTGGAGAG.........................................................................................................................................................................................	22	0	19	0.11	2	1	0	1	0
...............................................TTTGTGAGTGCCTGGAGAGACGTCA...................................................................................................................................................................................	25	1	10	0.10	1	0	0	1	0
......................ACACCGGACACCCCTCCGCGG................................................................................................................................................................................................................	21	0	11	0.09	1	0	0	1	0
................................................TTGTGAGTGTCTGGAGAGACGTCA...................................................................................................................................................................................	24	0	11	0.09	1	0	0	1	0
.................................................TGTGAGTGTCTGGAGAGACGTCAA..................................................................................................................................................................................	24	0	11	0.09	1	1	0	0	0
.............................................................................................................................................................................CGTCGGGGTCAATGAGAGT...........................................................	19	3	13	0.08	1	0	0	1	0
......................ACACCGGACACCCCTACGCG.................................................................................................................................................................................................................	20	1	14	0.07	1	1	0	0	0
...............................................................................................................................................................................TCAGGGTTAGTGACTCTCA.........................................................	19	3	14	0.07	1	1	0	0	0
............................................CCGTTTGTGAGTGTCTGGAGAGACG......................................................................................................................................................................................	25	0	18	0.06	1	1	0	0	0
......................................................................................................................................................................................................................CCCTGGACTTCGCCGAGC...................	18	3	20	0.05	1	1	0	0	0
.............................................CGTTTGTGAGTGTCTGGAGAG.........................................................................................................................................................................................	21	0	20	0.05	1	1	0	0	0
................................................TTGTGAGTGTCTGGAGAGACG......................................................................................................................................................................................	21	0	20	0.05	1	1	0	0	0
.............................................CGTTTGTGAGTGTCTGGAGAGA........................................................................................................................................................................................	22	0	20	0.05	1	1	0	0	0

##################################################---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
CAAGGATCAAGCGAGCGTCGAGACACCGGACACCCCTCCGCGGTCCGTTTGTGAGTGTCTGGAGAGACGTCAATCTCCAGAAGTCGTGGAAGCAGCTATGCCGGACACCCCTCTGCGGCCCGTGTATGAGCCGATAAGACCCCCAAGGAAAACAAAAGTGTTCTTCTGACAACCGTCAGGGTCAGTGAGACTCAGTCTGACAACGAGGAGATGCCCCTAGACTTCTCCGGGCCCCGCCCTCGACCATCGCT
***************************************************************..........(((((((.((..(((...(((....)))....)))..)))))((((.((....)).))))....(((((...(((((.((....)).))))).((((....)))))))))...))))..***********************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M045
female body

V117
male body

V118
embryo

V119
head

..........GAGAGCGTCGAGATCCCGGACAC..........................................................................................................................................................................................................................

2.00

..................................................................................GACGTTGAAGCAGCTATGC......................................................................................................................................................

2.00

...........AGAGCGTCGAGATCCCGGACACC.........................................................................................................................................................................................................................

1.00

....................................................................................CGTTGAAGCAGCTATGCG.....................................................................................................................................................

0.50

...............................................................GAGACGTCAATCTCCAGAAGTCGTGG..................................................................................................................................................................

0.33

...........................................................................................................................................................................ACCGTCAGGGTCAGTGAGACT...........................................................

0.33

........................................................................................................................................................................ACAACCGTCAGGGTCAGTGAGACTC..........................................................

0.33

....................................................................................................................................................................................................CTGACAACGAGGAGATCCCCCTAG...............................

0.33

................................................................................................................................................................TTCTTCTACCAACCGTCAG........................................................................

0.33

....................................................................................................................................................................................................CTGACAACGAGGAGATGCCCCTA................................

0.33

.................................................TGTGAGTGTCTGGAGAGACGTCA...................................................................................................................................................................................

0.27

......................................................................................................................................................................TGACAACCGTCAGGGTCAGTGAGAC............................................................

0.25

.................................................................................TGACGTTGAAGCAGCTATGC......................................................................................................................................................

0.22

............................................CCGTTTGTGAGTGTCTGGAGAGA........................................................................................................................................................................................

0.21

..................................................................................GACGTTGAAGCAGCTATGCG.....................................................................................................................................................

0.20

...........................................................................TCCAGAAGTCGTGGAAGCAGC...........................................................................................................................................................

0.18

.........................................................................................................CACCCCTCTGCGGCCCGTGTAT............................................................................................................................

0.17

..................................................................................................TGCCGGACACCCCTCTGCGGCCCGTG...............................................................................................................................

0.17

..............................................................AGAGACGTCAATCTCCAGAAGTCG.....................................................................................................................................................................

0.17

...........................................................................................................CCCCTCTGCGGCCCGTGTAT............................................................................................................................

0.17

.................................................................................................ATGCCGGACACCCCTCTGCGGCCCG.................................................................................................................................

0.14

.......................................................................AATCTCCAGAAGTCGTGGAAGCAG............................................................................................................................................................

0.13

........................................................................................................................................................................ACAACCGTCAGGGTCAGTGAGA.............................................................

0.13

........................................................................ATCTCCAGAAGTCGTGGAAGCAG............................................................................................................................................................

0.13

.................................................................................................................................................................................................................GATGCCCTTAGACTTCTG........................

0.13

........................................................................................................................................................................ACAACCGTCAGGGTCAGTGAG..............................................................

0.13

...............................................TTTGTGAGTGTCTGGAGAGACG......................................................................................................................................................................................

0.11

............................................CCGTTTGTGAGTGTCTGGAGAG.........................................................................................................................................................................................

0.11

...............................................TTTGTGAGTGCCTGGAGAGACGTCA...................................................................................................................................................................................

0.10

......................ACACCGGACACCCCTCCGCGG................................................................................................................................................................................................................

0.09

................................................TTGTGAGTGTCTGGAGAGACGTCA...................................................................................................................................................................................

0.09

.................................................TGTGAGTGTCTGGAGAGACGTCAA..................................................................................................................................................................................

0.09

.............................................................................................................................................................................CGTCGGGGTCAATGAGAGT...........................................................

0.08

......................ACACCGGACACCCCTACGCG.................................................................................................................................................................................................................

0.07

...............................................................................................................................................................................TCAGGGTTAGTGACTCTCA.........................................................

0.07

............................................CCGTTTGTGAGTGTCTGGAGAGACG......................................................................................................................................................................................

0.06

......................................................................................................................................................................................................................CCCTGGACTTCGCCGAGC...................

0.05

.............................................CGTTTGTGAGTGTCTGGAGAG.........................................................................................................................................................................................

0.05

................................................TTGTGAGTGTCTGGAGAGACG......................................................................................................................................................................................

0.05

.............................................CGTTTGTGAGTGTCTGGAGAGA........................................................................................................................................................................................

0.05

GTTCCTAGTTCGCTCGCAGCTCTGTGGCCTGTGGGGAGGCGCCAGGCAAACACTCACAGACCTCTCTGCAGTTAGAGGTCTTCAGCACCTTCGTCGATACGGCCTGTGGGGAGACGCCGGGCACATACTCGGCTATTCTGGGGGTTCCTTTTGTTTTCACAAGAAGACTGTTGGCAGTCCCAGTCACTCTGAGTCAGACTGTTGCTCCTCTACGGGGATCTGAAGAGGCCCGGGGCGGGAGCTGGTAGCGA *********************************************************..........(((((((.((..(((...(((....)))....)))..)))))((((.((....)).))))....(((((...(((((.((....)).))))).((((....)))))))))...))))..*************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M045 female body	V118 embryo	V117 male body	M020 head	V119 head
..................................................................TGCAGTTAGAGGTCTTCAGCACCTT................................................................................................................................................................	25	0	6	5.17	31	5	26	0	0	0
...................................................................GCAGTTAGAGGTCTTCAGCACCTT................................................................................................................................................................	24	0	6	2.50	15	12	3	0	0	0
.................................................................CTGCAGTTAGAGGTCTTCAGCACCTT................................................................................................................................................................	26	0	6	2.33	14	4	8	1	1	0
...........................................................ACCTCTCTGCAGTTAGAGGTCTTCAG......................................................................................................................................................................	26	0	6	2.17	13	13	0	0	0	0
...............................................................CTCTGCAGTTAGAGGTCTTCAGCACCT.................................................................................................................................................................	27	0	6	1.83	11	4	7	0	0	0
...................................................................GCAGTTAGAGGTCTTCAGCACCTTCGT.............................................................................................................................................................	27	0	6	1.50	9	8	1	0	0	0
.........................................................AGACCTCTCTGCAGTTAGAGGTCTTCAG......................................................................................................................................................................	28	0	6	1.33	8	8	0	0	0	0
.............TCGCAGCTCTAGGGCCTGTGGT........................................................................................................................................................................................................................	22	3	1	1.00	1	0	1	0	0	0
....................TCTGTGGTCTGTGGGGAGGCGC.................................................................................................................................................................................................................	22	1	1	1.00	1	0	1	0	0	0
...........GCTCGCAGCTCTAGGGCCTGT...........................................................................................................................................................................................................................	21	2	1	1.00	1	0	1	0	0	0
..................................................................TGCAGTTAGAGGTCTTCAGCACCT.................................................................................................................................................................	24	0	6	0.83	5	0	5	0	0	0
.............................................GCAAACACTCACAGACCTCTCTGCAGT...................................................................................................................................................................................	27	0	10	0.80	8	8	0	0	0	0
..............................................................TCTCTGCAGTTAGAGGTCTTCAGCACCT.................................................................................................................................................................	28	0	6	0.67	4	4	0	0	0	0
.....................................................................................................................................................................GACTGTTGGCAGTCCCAGTCACTCT.............................................................	25	0	8	0.50	4	4	0	0	0	0
............................................................CCTCTCTGCAGTTAGAGGTCTTCAG......................................................................................................................................................................	25	0	6	0.50	3	3	0	0	0	0
..........................................................GACCTCTCTGCAGTTAGAGGTCTTCAG......................................................................................................................................................................	27	0	6	0.50	3	3	0	0	0	0
.........................GGCCTGTTGGAAGGCGCTAGGCA...........................................................................................................................................................................................................	23	3	4	0.50	2	2	0	0	0	0
.................................................................CTGCAGTTAGAGGTCTTCAGCACCT.................................................................................................................................................................	25	0	6	0.50	3	3	0	0	0	0
..................................................................................................................................................................................................................................GGCCCGGGGCGGAGGCTG.......	18	2	7	0.43	3	3	0	0	0	0
.................................................................................................................................................................................................................................CGGCCCGGGGCGGAGGCTG.......	19	3	11	0.36	4	4	0	0	0	0
...................................................................................................................................................................................................AGACTGTTGCTCCTCTACGGGGATCT..............................	26	0	3	0.33	1	1	0	0	0	0
...............................................................CTCTGCAGTTAGAGGTCTTCAGCACCTT................................................................................................................................................................	28	0	6	0.33	2	2	0	0	0	0
.............................................................CTCTCTGCAGTTAGAGGTCTTCAGCACCT.................................................................................................................................................................	29	0	6	0.33	2	2	0	0	0	0
...........................................................................................................GGGGAGACGCCGGGCACATACT..........................................................................................................................	22	0	6	0.33	2	2	0	0	0	0
.........................................................................................................GTGGGGAGACGCCGGGCACATACT..........................................................................................................................	24	0	6	0.33	2	2	0	0	0	0
............................................GGCAAACACTCACAGACCTCTCTGCAGT...................................................................................................................................................................................	28	0	9	0.33	3	3	0	0	0	0
.............................................................CTCTCTGCAGTTAGAGGTCTTCAG......................................................................................................................................................................	24	0	6	0.33	2	1	1	0	0	0
..........................................................................................................TGGGGAGACGCCGGGCACATACT..........................................................................................................................	23	0	6	0.33	2	0	2	0	0	0
.................................................................CTGCAGTTAGAGGTCTTCAGCACCTTCGT.............................................................................................................................................................	29	0	6	0.33	2	2	0	0	0	0
...............................................................CTCTGCAGTTAGAGGTCTTCAGCAC...................................................................................................................................................................	25	0	6	0.33	2	2	0	0	0	0
..........................................................TACCTCTCTGCAGTTAGAGGTCTTCAG......................................................................................................................................................................	27	1	6	0.33	2	0	2	0	0	0
..............................................................TCTCTGCAGTTAGAGGTCTTCAGC.....................................................................................................................................................................	24	0	6	0.33	2	1	1	0	0	0
.............................................GCAAACACTCACAGACCTCTCTGCAGTT..................................................................................................................................................................................	28	0	10	0.30	3	3	0	0	0	0
................................................................................................................................................................................................................................GTTGCCCGGGGCGGGAGCTGGTAGCGA	27	2	4	0.25	1	0	1	0	0	0
...........................................................................................................................................................................TGGCGGTCGCAGTCACTCTAA...........................................................	21	3	4	0.25	1	1	0	0	0	0
..................................................................................................................................................................................................................................TGCCCGGGGCGGGAGCTGGTAGC..	23	1	4	0.25	1	0	1	0	0	0
.........................GGCCTGTTGGAAGGCGCTAGGC............................................................................................................................................................................................................	22	3	4	0.25	1	1	0	0	0	0
.......................................................................................................................................................................................................................................GGGGCGGGAGCTGGTAGCGA	20	0	4	0.25	1	0	1	0	0	0
....................................................................................................................................................................................................................................CCCGGGGCGGGAGCTGGTAGC..	21	0	4	0.25	1	0	1	0	0	0
...................................................................................................................................................................................................................................GCCCGGGGCGGGAGCTGGTAGCGA	24	0	4	0.25	1	0	1	0	0	0
................................................................TCTGCAGTTAGAGGTCTTCAGCACCT.................................................................................................................................................................	26	0	6	0.17	1	0	1	0	0	0
........................................................................................................ATTGGGGAGACGCCGGGCACATACT..........................................................................................................................	25	2	6	0.17	1	0	1	0	0	0
..............................................................TCTCTGCAGTTAGAGGTCTTCAG......................................................................................................................................................................	23	0	6	0.17	1	1	0	0	0	0
...................................................................GCAGTTAGAGGTCTTCAGCACTTTCGT.............................................................................................................................................................	27	1	6	0.17	1	0	1	0	0	0
...............................................................CTCTGCAGTTAGAGGTCTTCAGCT....................................................................................................................................................................	24	1	6	0.17	1	0	1	0	0	0
.................................................................CTGCAGTTAGAGGTCTTCAGCAC...................................................................................................................................................................	23	0	6	0.17	1	1	0	0	0	0
...........................................................ACCTCTCTGCAGTTAGAGGTCTTCAGCA....................................................................................................................................................................	28	0	6	0.17	1	1	0	0	0	0
.........................................................AGACCTCTCTGCAGTTAGAGGTCTTCA.......................................................................................................................................................................	27	0	6	0.17	1	1	0	0	0	0
...........................................................................................................GGGGAGACGCCGGGCACATAC...........................................................................................................................	21	0	6	0.17	1	0	0	1	0	0
..................................................................TGCAGTTAGAGGTCTTCAGCACC..................................................................................................................................................................	23	0	6	0.17	1	1	0	0	0	0
...................................................................GCAGTTAGAGGTCTTCAGC.....................................................................................................................................................................	19	0	6	0.17	1	1	0	0	0	0
...........................................................ACCTCTCTGCAGTTAGAGGTCTCCAG......................................................................................................................................................................	26	1	6	0.17	1	1	0	0	0	0
.............................................................................................................GGAGACGCCGGGCACATACT..........................................................................................................................	20	0	6	0.17	1	1	0	0	0	0
..................................................................TGCAGTTAGAGGTCTTCAGCACCTTCTT.............................................................................................................................................................	28	1	6	0.17	1	1	0	0	0	0
..............................................................TCTCTGCAGTTAGAGGTCTTCAGCAC...................................................................................................................................................................	26	0	6	0.17	1	0	1	0	0	0
......................................................................................................CCTGTGGGGAGACGCCGGGCACATAC...........................................................................................................................	26	0	6	0.17	1	0	1	0	0	0
...................................................................GCAGTTAGAGGTCTTCAGCACCT.................................................................................................................................................................	23	0	6	0.17	1	1	0	0	0	0
.................................................................CTGCAGTTAGAGGACTTCAGCACCTT................................................................................................................................................................	26	1	6	0.17	1	0	1	0	0	0
............................................................................................................GGGAGACGCCGGGCACATACT..........................................................................................................................	21	0	6	0.17	1	1	0	0	0	0
...............................................................CTCTGCAGTTAGAGGTCTTCAGCACC..................................................................................................................................................................	26	0	6	0.17	1	1	0	0	0	0
...................................................................GCAGTTAGAGGTCTTCAGCACCTTCG..............................................................................................................................................................	26	0	6	0.17	1	1	0	0	0	0
..................................................................TGCAGTTAGAGGTCTTCAGCACCTTCGT.............................................................................................................................................................	28	0	6	0.17	1	1	0	0	0	0
.............................................................CTCTCTGCAGTTAGAGGTCTTCAGCACC..................................................................................................................................................................	28	0	6	0.17	1	1	0	0	0	0
.................................................................CTGCAGTTAGAGGCCTTCAGCACCTT................................................................................................................................................................	26	1	6	0.17	1	0	1	0	0	0
...................................................................GCAGTTAGATGTCTTCAGCACCTT................................................................................................................................................................	24	1	6	0.17	1	1	0	0	0	0
.........................................................AGACCTCTCTGCAGTTAGAGGT............................................................................................................................................................................	22	0	7	0.14	1	0	1	0	0	0
.......................................................................................................TTGTGGGGAGACGCCGGGCACATACT..........................................................................................................................	26	1	7	0.14	1	1	0	0	0	0
.....................................................................AGTTAGAGGTCTTCAGCACCTTCGTC............................................................................................................................................................	26	0	7	0.14	1	0	1	0	0	0
......................................................................GTTAGAGGTCTTCAGCACCTTCGT.............................................................................................................................................................	24	0	7	0.14	1	1	0	0	0	0
......................................................................................................TTTGTGGGGAGACGCCGGGCACATACT..........................................................................................................................	27	2	7	0.14	1	0	1	0	0	0
.....................................................................................................................................................................TACTGTTGGCAGTCCCAGTCACTCT.............................................................	25	1	8	0.13	1	1	0	0	0	0
......................................................................................................................................................................ACTGTTGGCAGTCCCAGTCACTCT.............................................................	24	0	8	0.13	1	1	0	0	0	0
............................................GGCAAACACTCACAGACCTCTCTGCAG....................................................................................................................................................................................	27	0	17	0.12	2	2	0	0	0	0
............................................GGCAAACACTCACAGACCTCTCTGCAGTT..................................................................................................................................................................................	29	0	9	0.11	1	1	0	0	0	0
.............................................GCAAACACTCACAGACCTCTCTGCAG....................................................................................................................................................................................	26	0	18	0.11	2	2	0	0	0	0
..............................................CAAACACTCACAGACCTCTCTGCAGT...................................................................................................................................................................................	26	0	10	0.10	1	1	0	0	0	0
.........................GGCCTGTTGGAAGGCGCTA...............................................................................................................................................................................................................	19	3	20	0.05	1	1	0	0	0	0
.................................................ACACTCACAGACCTCTCT........................................................................................................................................................................................	18	0	20	0.05	1	1	0	0	0	0
........................................................................................................................................................................................................................................AGGGGTGAGCTGGTAGCGA	19	3	20	0.05	1	0	0	0	0	1
......................................................................................................................................................................................................................................CGCGGCGGGAACGGGTAGC..	19	3	20	0.05	1	1	0	0	0	0
...................................................................................................................................................................................................................................GCCCGGGGCGGAGGCTGA......	18	3	20	0.05	1	0	0	1	0	0

GTTCCTAGTTCGCTCGCAGCTCTGTGGCCTGTGGGGAGGCGCCAGGCAAACACTCACAGACCTCTCTGCAGTTAGAGGTCTTCAGCACCTTCGTCGATACGGCCTGTGGGGAGACGCCGGGCACATACTCGGCTATTCTGGGGGTTCCTTTTGTTTTCACAAGAAGACTGTTGGCAGTCCCAGTCACTCTGAGTCAGACTGTTGCTCCTCTACGGGGATCTGAAGAGGCCCGGGGCGGGAGCTGGTAGCGA
***********************************************************..........(((((((.((..(((...(((....)))....)))..)))))((((.((....)).))))....(((((...(((((.((....)).))))).((((....)))))))))...))))..***************************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M045
female body

V118
embryo

V117
male body

M020
head

V119
head

..................................................................TGCAGTTAGAGGTCTTCAGCACCTT................................................................................................................................................................

5.17

...................................................................GCAGTTAGAGGTCTTCAGCACCTT................................................................................................................................................................

2.50

.................................................................CTGCAGTTAGAGGTCTTCAGCACCTT................................................................................................................................................................

2.33

...........................................................ACCTCTCTGCAGTTAGAGGTCTTCAG......................................................................................................................................................................

2.17

...............................................................CTCTGCAGTTAGAGGTCTTCAGCACCT.................................................................................................................................................................

1.83

...................................................................GCAGTTAGAGGTCTTCAGCACCTTCGT.............................................................................................................................................................

1.50

.........................................................AGACCTCTCTGCAGTTAGAGGTCTTCAG......................................................................................................................................................................

1.33

.............TCGCAGCTCTAGGGCCTGTGGT........................................................................................................................................................................................................................

1.00

....................TCTGTGGTCTGTGGGGAGGCGC.................................................................................................................................................................................................................

1.00

...........GCTCGCAGCTCTAGGGCCTGT...........................................................................................................................................................................................................................

1.00

..................................................................TGCAGTTAGAGGTCTTCAGCACCT.................................................................................................................................................................

0.83

.............................................GCAAACACTCACAGACCTCTCTGCAGT...................................................................................................................................................................................

0.80

..............................................................TCTCTGCAGTTAGAGGTCTTCAGCACCT.................................................................................................................................................................

0.67

.....................................................................................................................................................................GACTGTTGGCAGTCCCAGTCACTCT.............................................................

0.50

............................................................CCTCTCTGCAGTTAGAGGTCTTCAG......................................................................................................................................................................

0.50

..........................................................GACCTCTCTGCAGTTAGAGGTCTTCAG......................................................................................................................................................................

0.50

.........................GGCCTGTTGGAAGGCGCTAGGCA...........................................................................................................................................................................................................

0.50

.................................................................CTGCAGTTAGAGGTCTTCAGCACCT.................................................................................................................................................................

0.50

..................................................................................................................................................................................................................................GGCCCGGGGCGGAGGCTG.......

0.43

.................................................................................................................................................................................................................................CGGCCCGGGGCGGAGGCTG.......

0.36

...................................................................................................................................................................................................AGACTGTTGCTCCTCTACGGGGATCT..............................

0.33

...............................................................CTCTGCAGTTAGAGGTCTTCAGCACCTT................................................................................................................................................................

0.33

.............................................................CTCTCTGCAGTTAGAGGTCTTCAGCACCT.................................................................................................................................................................

0.33

...........................................................................................................GGGGAGACGCCGGGCACATACT..........................................................................................................................

0.33

.........................................................................................................GTGGGGAGACGCCGGGCACATACT..........................................................................................................................

0.33

............................................GGCAAACACTCACAGACCTCTCTGCAGT...................................................................................................................................................................................

0.33

.............................................................CTCTCTGCAGTTAGAGGTCTTCAG......................................................................................................................................................................

0.33

..........................................................................................................TGGGGAGACGCCGGGCACATACT..........................................................................................................................

0.33

.................................................................CTGCAGTTAGAGGTCTTCAGCACCTTCGT.............................................................................................................................................................

0.33

...............................................................CTCTGCAGTTAGAGGTCTTCAGCAC...................................................................................................................................................................

0.33

..........................................................TACCTCTCTGCAGTTAGAGGTCTTCAG......................................................................................................................................................................

0.33

..............................................................TCTCTGCAGTTAGAGGTCTTCAGC.....................................................................................................................................................................

0.33

.............................................GCAAACACTCACAGACCTCTCTGCAGTT..................................................................................................................................................................................

0.30

................................................................................................................................................................................................................................GTTGCCCGGGGCGGGAGCTGGTAGCGA

0.25

...........................................................................................................................................................................TGGCGGTCGCAGTCACTCTAA...........................................................

0.25

..................................................................................................................................................................................................................................TGCCCGGGGCGGGAGCTGGTAGC..

0.25

.........................GGCCTGTTGGAAGGCGCTAGGC............................................................................................................................................................................................................

0.25

.......................................................................................................................................................................................................................................GGGGCGGGAGCTGGTAGCGA

0.25

....................................................................................................................................................................................................................................CCCGGGGCGGGAGCTGGTAGC..

0.25

...................................................................................................................................................................................................................................GCCCGGGGCGGGAGCTGGTAGCGA

0.25

................................................................TCTGCAGTTAGAGGTCTTCAGCACCT.................................................................................................................................................................

0.17

........................................................................................................ATTGGGGAGACGCCGGGCACATACT..........................................................................................................................

0.17

..............................................................TCTCTGCAGTTAGAGGTCTTCAG......................................................................................................................................................................

0.17

...................................................................GCAGTTAGAGGTCTTCAGCACTTTCGT.............................................................................................................................................................

0.17

...............................................................CTCTGCAGTTAGAGGTCTTCAGCT....................................................................................................................................................................

0.17

.................................................................CTGCAGTTAGAGGTCTTCAGCAC...................................................................................................................................................................

0.17

...........................................................ACCTCTCTGCAGTTAGAGGTCTTCAGCA....................................................................................................................................................................

0.17

.........................................................AGACCTCTCTGCAGTTAGAGGTCTTCA.......................................................................................................................................................................

0.17

...........................................................................................................GGGGAGACGCCGGGCACATAC...........................................................................................................................

0.17

..................................................................TGCAGTTAGAGGTCTTCAGCACC..................................................................................................................................................................

0.17

...................................................................GCAGTTAGAGGTCTTCAGC.....................................................................................................................................................................

0.17

...........................................................ACCTCTCTGCAGTTAGAGGTCTCCAG......................................................................................................................................................................

0.17

.............................................................................................................GGAGACGCCGGGCACATACT..........................................................................................................................

0.17

..................................................................TGCAGTTAGAGGTCTTCAGCACCTTCTT.............................................................................................................................................................

0.17

..............................................................TCTCTGCAGTTAGAGGTCTTCAGCAC...................................................................................................................................................................

0.17

......................................................................................................CCTGTGGGGAGACGCCGGGCACATAC...........................................................................................................................

0.17

...................................................................GCAGTTAGAGGTCTTCAGCACCT.................................................................................................................................................................

0.17

.................................................................CTGCAGTTAGAGGACTTCAGCACCTT................................................................................................................................................................

0.17

............................................................................................................GGGAGACGCCGGGCACATACT..........................................................................................................................

0.17

...............................................................CTCTGCAGTTAGAGGTCTTCAGCACC..................................................................................................................................................................

0.17

...................................................................GCAGTTAGAGGTCTTCAGCACCTTCG..............................................................................................................................................................

0.17

..................................................................TGCAGTTAGAGGTCTTCAGCACCTTCGT.............................................................................................................................................................

0.17

.............................................................CTCTCTGCAGTTAGAGGTCTTCAGCACC..................................................................................................................................................................

0.17

.................................................................CTGCAGTTAGAGGCCTTCAGCACCTT................................................................................................................................................................

0.17

...................................................................GCAGTTAGATGTCTTCAGCACCTT................................................................................................................................................................

0.17

.........................................................AGACCTCTCTGCAGTTAGAGGT............................................................................................................................................................................

0.14

.......................................................................................................TTGTGGGGAGACGCCGGGCACATACT..........................................................................................................................

0.14

.....................................................................AGTTAGAGGTCTTCAGCACCTTCGTC............................................................................................................................................................

0.14

......................................................................GTTAGAGGTCTTCAGCACCTTCGT.............................................................................................................................................................

0.14

......................................................................................................TTTGTGGGGAGACGCCGGGCACATACT..........................................................................................................................

0.14

.....................................................................................................................................................................TACTGTTGGCAGTCCCAGTCACTCT.............................................................

0.13

......................................................................................................................................................................ACTGTTGGCAGTCCCAGTCACTCT.............................................................

0.13

............................................GGCAAACACTCACAGACCTCTCTGCAG....................................................................................................................................................................................

0.12

............................................GGCAAACACTCACAGACCTCTCTGCAGTT..................................................................................................................................................................................

0.11

.............................................GCAAACACTCACAGACCTCTCTGCAG....................................................................................................................................................................................

0.11

..............................................CAAACACTCACAGACCTCTCTGCAGT...................................................................................................................................................................................

0.10

.........................GGCCTGTTGGAAGGCGCTA...............................................................................................................................................................................................................

0.05

.................................................ACACTCACAGACCTCTCT........................................................................................................................................................................................

0.05

........................................................................................................................................................................................................................................AGGGGTGAGCTGGTAGCGA

0.05

......................................................................................................................................................................................................................................CGCGGCGGGAACGGGTAGC..

0.05

...................................................................................................................................................................................................................................GCCCGGGGCGGAGGCTGA......

0.05

Species	Coordinate	ID	Alignment
droWil2	scf2_1100000004397:5774-6024 -	dwi_355	CAAGGATCAAGCGAGCGTCGAGACACCGGACACCCCTCCGCGGTCCGTTTGTGAGTGTCTGGAGAGACGTCAATCTCCAGAAGTCGTGGAAGCAGCTATGCCGGACACCCCTCTGCGGCCCGTGTATGAGCCGATAAGACCCCCAAGGAAAACAAAAGTGTTCTTCTGACAACCGTCAGGGTCAGTGAGACTCAGTCTGACAACGAGGAGATGCCCCTAGACTTCTCCGGGCCCCGCCCTCGACCATCGCT
droFic1	scf7180000453567:14624-14666 +		C-----------------------T---------------------------------------------------CCAGGAGACGCTCCATCAGCGACGTCGGACACCGCTTTGCG--------------------------------------------------------------------------------------------------------------------------------------
droTak1	scf7180000414060:7825-7863 +		C-----------------------CCCAGACCGCCGTCCTGGGT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTACCTCACCGATGGCT

Species

Coordinate

Alignment

droWil2

scf2_1100000004397:5774-6024 -

dwi_355

CAAGGATCAAGCGAGCGTCGAGACACCGGACACCCCTCCGCGGTCCGTTTGTGAGTGTCTGGAGAGACGTCAATCTCCAGAAGTCGTGGAAGCAGCTATGCCGGACACCCCTCTGCGGCCCGTGTATGAGCCGATAAGACCCCCAAGGAAAACAAAAGTGTTCTTCTGACAACCGTCAGGGTCAGTGAGACTCAGTCTGACAACGAGGAGATGCCCCTAGACTTCTCCGGGCCCCGCCCTCGACCATCGCT

droFic1

scf7180000453567:14624-14666 +

C-----------------------T---------------------------------------------------CCAGGAGACGCTCCATCAGCGACGTCGGACACCGCTTTGCG--------------------------------------------------------------------------------------------------------------------------------------

droTak1

scf7180000414060:7825-7863 +

C-----------------------CCCAGACCGCCGTCCTGGGT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTACCTCACCGATGGCT

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droWil2

CAAGGATCAAGCGAGCGTCGAGACACCGGACACCCCTCCGCGGTCCGTTTGTGAGTGTCTGGAGAGACGTCAATCTCCAGAAGTCGTGGAAGCAGCTATGCCGGACACCCCTCTGCGGCCCGTGTATGAGCCGATAAGACCCCCAAGGAAAACAAAAGTGTTCTTCTGACAACCGTCAGGGTCAGTGAGACTCAGTCTGACAACGAGGAGATGCCCCTAGACTTCTCCGGGCCCCGCCCTCGACCATCGCT	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
...............................................................GAGACGTCAATCTCCAGAAGTCGTGG..................................................................................................................................................................	26	6	0.33	2	0	2	0	0	0
...........................................................................................................................................................................ACCGTCAGGGTCAGTGAGACT...........................................................	21	3	0.33	1	0	1	0	0	0
........................................................................................................................................................................ACAACCGTCAGGGTCAGTGAGACTC..........................................................	25	3	0.33	1	0	1	0	0	0
....................................................................................................................................................................................................CTGACAACGAGGAGATGCCCCTA................................	23	3	0.33	1	0	1	0	0	0
.................................................TGTGAGTGTCTGGAGAGACGTCA...................................................................................................................................................................................	23	11	0.27	3	0	2	1	0	0
......................................................................................................................................................................TGACAACCGTCAGGGTCAGTGAGAC............................................................	25	8	0.25	2	0	1	0	1	0
............................................CCGTTTGTGAGTGTCTGGAGAGA........................................................................................................................................................................................	23	19	0.21	4	0	3	0	1	0
...........................................................................TCCAGAAGTCGTGGAAGCAGC...........................................................................................................................................................	21	11	0.18	2	0	1	0	1	0
.........................................................................................................CACCCCTCTGCGGCCCGTGTAT............................................................................................................................	22	6	0.17	1	0	1	0	0	0
..................................................................................................TGCCGGACACCCCTCTGCGGCCCGTG...............................................................................................................................	26	6	0.17	1	0	1	0	0	0
..............................................................AGAGACGTCAATCTCCAGAAGTCG.....................................................................................................................................................................	24	6	0.17	1	0	1	0	0	0
...........................................................................................................CCCCTCTGCGGCCCGTGTAT............................................................................................................................	20	6	0.17	1	0	1	0	0	0
.................................................................................................ATGCCGGACACCCCTCTGCGGCCCG.................................................................................................................................	25	7	0.14	1	0	1	0	0	0
.......................................................................AATCTCCAGAAGTCGTGGAAGCAG............................................................................................................................................................	24	8	0.13	1	0	1	0	0	0
........................................................................................................................................................................ACAACCGTCAGGGTCAGTGAGA.............................................................	22	8	0.13	1	0	1	0	0	0
........................................................................ATCTCCAGAAGTCGTGGAAGCAG............................................................................................................................................................	23	8	0.13	1	0	1	0	0	0
........................................................................................................................................................................ACAACCGTCAGGGTCAGTGAG..............................................................	21	8	0.13	1	0	1	0	0	0
...............................................TTTGTGAGTGTCTGGAGAGACG......................................................................................................................................................................................	22	19	0.11	2	0	1	0	1	0
............................................CCGTTTGTGAGTGTCTGGAGAG.........................................................................................................................................................................................	22	19	0.11	2	0	1	0	1	0
......................ACACCGGACACCCCTCCGCGG................................................................................................................................................................................................................	21	11	0.09	1	0	0	0	1	0
................................................TTGTGAGTGTCTGGAGAGACGTCA...................................................................................................................................................................................	24	11	0.09	1	0	0	0	1	0
.................................................TGTGAGTGTCTGGAGAGACGTCAA..................................................................................................................................................................................	24	11	0.09	1	0	1	0	0	0
............................................CCGTTTGTGAGTGTCTGGAGAGACG......................................................................................................................................................................................	25	18	0.06	1	0	1	0	0	0
.............................................CGTTTGTGAGTGTCTGGAGAG.........................................................................................................................................................................................	21	20	0.05	1	0	1	0	0	0
................................................TTGTGAGTGTCTGGAGAGACG......................................................................................................................................................................................	21	20	0.05	1	0	1	0	0	0
.............................................CGTTTGTGAGTGTCTGGAGAGA........................................................................................................................................................................................	22	20	0.05	1	0	1	0	0	0

droFic1

C-----------------------T---------------------------------------------------CCAGGAGACGCTCCATCAGCGACGTCGGACACCGCTTTGCG--------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droTak1

C-----------------------CCCAGACCGCCGTCCTGGGT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTACCTCACCGATGGCT	Size	Hit Count	Total Norm	Total
No Reads

dwi_355

scf2_1100000004397:5824-5974 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-29.3	-29.3	-29.3