dwi_3517

exon [dwil_GLEANR_14737:2]; CDS [Dwil\GK14419-cds]; CDS [Dwil\GK14419-cds]; exon [dwil_GLEANR_14737:3]; intron [Dwil\GK14419-in]

##################################################---------------------------------------------------------------################################################## ACGCAAAAAGCGTTTGTCCAAAAAGCGAGCAAGATACTTCATCTGAGCTTGTAAGTACCTACATATTTTGCATTTCGAATTTCAAAATTATATTTCATTGTGCTTCATTGCAGCTGAACAATTTTGATGAAGTTGCTGACATCTTAATGCAAGTGCCCATACC ************************************************...........((......((((...(((..............)))...)))).....))...************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M045 female body	V118 embryo	M020 head
........................................................................................TATATTTCATTGTGCTTCATTGCAGC.................................................	26	0	3	10.67	32	28	4	0
............................................................................................................TGCAGCTGAACAATTTTGATGAAGT..............................	25	0	1	6.00	6	3	3	0
..................................TACTTCATCTGAGCTTGTAAGTACC........................................................................................................	25	0	1	4.00	4	4	0	0
...............................................................................................................................TGAAGTTGCTGACATCTTAATGCAA...........	25	0	1	3.00	3	0	3	0
.....................................TTCATCTGAGCTTGTAAGTACCTACA....................................................................................................	26	0	1	3.00	3	2	1	0
.............TTGTCCAAAAAGCGAGCAAGATACT.............................................................................................................................	25	0	1	2.00	2	2	0	0
......................................TCATCTGAGCTTGTAAGTACCTACA....................................................................................................	25	0	1	2.00	2	1	1	0
.......................................CATCTGAGCTTGTAAGTACCTACA....................................................................................................	24	0	1	2.00	2	2	0	0
..........................................................................................TATTTCATTGTGCTTCATTGCAGC.................................................	24	0	3	1.67	5	5	0	0
.......................................................................................TTATATTTCATTGTGCTTCATTGCAGC.................................................	27	0	3	1.33	4	3	1	0
.........................................................................................................CATTTCAGCTGAACAATTTTGATG..................................	24	1	1	1.00	1	1	0	0
.........................................TCTGAGCTTGTAAGTACCTACATATTT...............................................................................................	27	0	1	1.00	1	1	0	0
.........................................................................................ATATTTCATTGTGCTTCATTGCAGC.................................................	25	0	3	1.00	3	3	0	0
...........................................................................................................TTGCAGCTGAACAATTTTGATGAAGT..............................	26	0	1	1.00	1	0	1	0
......................................................................................................................CAATTTTGATGAAGTTGCTGACA......................	23	0	1	1.00	1	1	0	0
..............................................................................................TCATTGTGCTTCATTGCAGCTGAACA...........................................	26	0	1	1.00	1	1	0	0
............................................................................................................TGCAGCTGAACAATTTTGAAA..................................	21	2	1	1.00	1	0	1	0
.......................AGCGAGCAAGATACTTCATCTGAGC...................................................................................................................	25	0	1	1.00	1	0	1	0
..............TGTCCAAAAAGCGAGCAAGATACT.............................................................................................................................	24	0	1	1.00	1	1	0	0
............................................................................................................TGCAGCTGAACAATTTTGATGAAGTT.............................	26	0	1	1.00	1	1	0	0
......AAAGCGTTTGTCCAAAAAGCGAGCAAGA.................................................................................................................................	28	0	1	1.00	1	1	0	0
..........................................................................................TATTTCATTGTGCTTCATTGCAGCTGA..............................................	27	0	3	1.00	3	1	2	0
...............................................................................TTTCAAAATTATATTTCATTGTGCT...........................................................	25	0	1	1.00	1	0	1	0
...............................AGATACTTCATCTGAGCTTGTAAGTACC........................................................................................................	28	0	1	1.00	1	1	0	0
............................................................................................................TGCAGCTGAACAATTTTGATGAAGTTA............................	27	1	1	1.00	1	1	0	0
.......................................................................................TTATATTTCATTGTGCTTCATTGC....................................................	24	0	3	0.33	1	1	0	0
.......................................................................................TTATATTTCATTCTGCTTCATTGCA...................................................	25	1	3	0.33	1	1	0	0
........................................................................................TATATTTCATTGTGCTTCATTGCAGCT................................................	27	0	3	0.33	1	1	0	0
...........................................................................................ATTTCATTGTGCTTCATTGCAGC.................................................	23	0	3	0.33	1	1	0	0
...............................................................................................CATTGTGCTTCATTGCAGC.................................................	19	0	3	0.33	1	1	0	0
..........................................................................................TATTTCATTGTGCTTCATTGCAGCT................................................	25	0	3	0.33	1	0	1	0
.......................................................................................TTATATTTCATGGTGCTTCATTGCAGC.................................................	27	1	3	0.33	1	1	0	0
.............................................................................................TTCATTGTGCTTCATTGCAGCTG...............................................	23	0	3	0.33	1	1	0	0
..........................................................................................TATTTCATTGTGCTTCATTGCAGCA................................................	25	1	3	0.33	1	1	0	0
..........................................................................................TATTTCATTGTGCTTCATTGCAGCTG...............................................	26	0	3	0.33	1	1	0	0
.........................................................................................ATATTTCATTGTGCTTCATTGCAG..................................................	24	0	3	0.33	1	1	0	0
........................................................................................TATATTTCATTGTGCTTCAT.......................................................	20	0	3	0.33	1	0	1	0
..........................................................................................TATTTCATTGTGCTTCATTGCAG..................................................	23	0	3	0.33	1	0	1	0
..............................................................................................TCATTGTGCTTCATTGCAGCT................................................	21	0	3	0.33	1	1	0	0
........................................................................................TATATTTCATTATGCTTCATTGCAGC.................................................	26	1	6	0.17	1	1	0	0
............................................................................................................................................ATCTTAATGCAAGTGCCC.....	18	0	9	0.11	1	1	0	0
................................................................................................ATTGTGTTTCATTGGAGC.................................................	18	2	20	0.05	1	0	0	1

##################################################---------------------------------------------------------------##################################################
ACGCAAAAAGCGTTTGTCCAAAAAGCGAGCAAGATACTTCATCTGAGCTTGTAAGTACCTACATATTTTGCATTTCGAATTTCAAAATTATATTTCATTGTGCTTCATTGCAGCTGAACAATTTTGATGAAGTTGCTGACATCTTAATGCAAGTGCCCATACC
**************************************************...........((......((((...(((..............)))...)))).....))...**************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M045
female body

V118
embryo

M020
head

........................................................................................TATATTTCATTGTGCTTCATTGCAGC.................................................

10.67

............................................................................................................TGCAGCTGAACAATTTTGATGAAGT..............................

6.00

..................................TACTTCATCTGAGCTTGTAAGTACC........................................................................................................

4.00

...............................................................................................................................TGAAGTTGCTGACATCTTAATGCAA...........

3.00

.....................................TTCATCTGAGCTTGTAAGTACCTACA....................................................................................................

3.00

.............TTGTCCAAAAAGCGAGCAAGATACT.............................................................................................................................

2.00

......................................TCATCTGAGCTTGTAAGTACCTACA....................................................................................................

2.00

.......................................CATCTGAGCTTGTAAGTACCTACA....................................................................................................

2.00

..........................................................................................TATTTCATTGTGCTTCATTGCAGC.................................................

1.67

.......................................................................................TTATATTTCATTGTGCTTCATTGCAGC.................................................

1.33

.........................................................................................................CATTTCAGCTGAACAATTTTGATG..................................

1.00

.........................................TCTGAGCTTGTAAGTACCTACATATTT...............................................................................................

1.00

.........................................................................................ATATTTCATTGTGCTTCATTGCAGC.................................................

1.00

...........................................................................................................TTGCAGCTGAACAATTTTGATGAAGT..............................

1.00

......................................................................................................................CAATTTTGATGAAGTTGCTGACA......................

1.00

..............................................................................................TCATTGTGCTTCATTGCAGCTGAACA...........................................

1.00

............................................................................................................TGCAGCTGAACAATTTTGAAA..................................

1.00

.......................AGCGAGCAAGATACTTCATCTGAGC...................................................................................................................

1.00

..............TGTCCAAAAAGCGAGCAAGATACT.............................................................................................................................

1.00

............................................................................................................TGCAGCTGAACAATTTTGATGAAGTT.............................

1.00

......AAAGCGTTTGTCCAAAAAGCGAGCAAGA.................................................................................................................................

1.00

..........................................................................................TATTTCATTGTGCTTCATTGCAGCTGA..............................................

1.00

...............................................................................TTTCAAAATTATATTTCATTGTGCT...........................................................

1.00

...............................AGATACTTCATCTGAGCTTGTAAGTACC........................................................................................................

1.00

............................................................................................................TGCAGCTGAACAATTTTGATGAAGTTA............................

1.00

.......................................................................................TTATATTTCATTGTGCTTCATTGC....................................................

0.33

.......................................................................................TTATATTTCATTCTGCTTCATTGCA...................................................

0.33

........................................................................................TATATTTCATTGTGCTTCATTGCAGCT................................................

0.33

...........................................................................................ATTTCATTGTGCTTCATTGCAGC.................................................

0.33

...............................................................................................CATTGTGCTTCATTGCAGC.................................................

0.33

..........................................................................................TATTTCATTGTGCTTCATTGCAGCT................................................

0.33

.......................................................................................TTATATTTCATGGTGCTTCATTGCAGC.................................................

0.33

.............................................................................................TTCATTGTGCTTCATTGCAGCTG...............................................

0.33

..........................................................................................TATTTCATTGTGCTTCATTGCAGCA................................................

0.33

..........................................................................................TATTTCATTGTGCTTCATTGCAGCTG...............................................

0.33

.........................................................................................ATATTTCATTGTGCTTCATTGCAG..................................................

0.33

........................................................................................TATATTTCATTGTGCTTCAT.......................................................

0.33

..........................................................................................TATTTCATTGTGCTTCATTGCAG..................................................

0.33

..............................................................................................TCATTGTGCTTCATTGCAGCT................................................

0.33

........................................................................................TATATTTCATTATGCTTCATTGCAGC.................................................

0.17

............................................................................................................................................ATCTTAATGCAAGTGCCC.....

0.11

................................................................................................ATTGTGTTTCATTGGAGC.................................................

0.05

TGCGTTTTTCGCAAACAGGTTTTTCGCTCGTTCTATGAAGTAGACTCGAACATTCATGGATGTATAAAACGTAAAGCTTAAAGTTTTAATATAAAGTAACACGAAGTAACGTCGACTTGTTAAAACTACTTCAACGACTGTAGAATTACGTTCACGGGTATGG ************************************************...........((......((((...(((..............)))...)))).....))...************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M045 female body	V117 male body
..................................................CATTCATGGATGTATAAAACGTA..........................................................................................	23	0	1	1.00	1	1	0
.....TTTTCGCAAACAGGTTTTTCGCTCGT....................................................................................................................................	26	0	1	1.00	1	1	0
......................TTCGCTCGTTCTATGAAGTAGACTC....................................................................................................................	25	0	1	1.00	1	1	0
........TCGCAAACAGGTTTTTCGCTCGTTC..................................................................................................................................	25	0	1	1.00	1	1	0
..............ACAGGTTTTTCGCTCGTTCTATGAAGT..........................................................................................................................	27	0	1	1.00	1	1	0
.........CGCAAATAGGTGTTTCGC........................................................................................................................................	18	2	3	0.33	1	1	0
..........GCAAACAGGCTTTTCGGTTG.....................................................................................................................................	20	3	10	0.10	1	0	1
..............................................................................................AGCAACATGAAGTAACATCG.................................................	20	3	19	0.05	1	1	0
....................................................................ACGTAAAGCTTCAAATTT.............................................................................	18	2	20	0.05	1	1	0

TGCGTTTTTCGCAAACAGGTTTTTCGCTCGTTCTATGAAGTAGACTCGAACATTCATGGATGTATAAAACGTAAAGCTTAAAGTTTTAATATAAAGTAACACGAAGTAACGTCGACTTGTTAAAACTACTTCAACGACTGTAGAATTACGTTCACGGGTATGG
**************************************************...........((......((((...(((..............)))...)))).....))...**************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M045
female body

V117
male body

..................................................CATTCATGGATGTATAAAACGTA..........................................................................................

1.00

.....TTTTCGCAAACAGGTTTTTCGCTCGT....................................................................................................................................

1.00

......................TTCGCTCGTTCTATGAAGTAGACTC....................................................................................................................

1.00

........TCGCAAACAGGTTTTTCGCTCGTTC..................................................................................................................................

1.00

..............ACAGGTTTTTCGCTCGTTCTATGAAGT..........................................................................................................................

1.00

.........CGCAAATAGGTGTTTCGC........................................................................................................................................

0.33

..........GCAAACAGGCTTTTCGGTTG.....................................................................................................................................

0.10

..............................................................................................AGCAACATGAAGTAACATCG.................................................

0.05

....................................................................ACGTAAAGCTTCAAATTT.............................................................................

0.05

Species	Coordinate	ID	Alignment
droWil2	scf2_1100000004943:15044155-15044317 +	dwi_3517	ACGCAAAAAGCGTTTGTCCAAAAAGCGAGCAAGATACTTCATCTGAGCTTGTAAGTACCTACATATTTTGCATTTCGAATTTCAAAATTATATTTCATTGTGCTTCATTGCAGCTGAACAATTTTGATGAAGTTGCTGACATCTTAATGCAAGTGCCCATACC

Species

Coordinate

Alignment

droWil2

scf2_1100000004943:15044155-15044317 +

dwi_3517

ACGCAAAAAGCGTTTGTCCAAAAAGCGAGCAAGATACTTCATCTGAGCTTGTAAGTACCTACATATTTTGCATTTCGAATTTCAAAATTATATTTCATTGTGCTTCATTGCAGCTGAACAATTTTGATGAAGTTGCTGACATCTTAATGCAAGTGCCCATACC

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droWil2

ACGCAAAAAGCGTTTGTCCAAAAAGCGAGCAAGATACTTCATCTGAGCTTGTAAGTACCTACATATTTTGCATTTCGAATTTCAAAATTATATTTCATTGTGCTTCATTGCAGCTGAACAATTTTGATGAAGTTGCTGACATCTTAATGCAAGTGCCCATACC	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
........................................................................................TATATTTCATTGTGCTTCATTGCAGC.................................................	26	3	10.67	32	0	28	0	4	0
............................................................................................................TGCAGCTGAACAATTTTGATGAAGT..............................	25	1	6.00	6	0	3	0	3	0
..................................TACTTCATCTGAGCTTGTAAGTACC........................................................................................................	25	1	4.00	4	0	4	0	0	0
...............................................................................................................................TGAAGTTGCTGACATCTTAATGCAA...........	25	1	3.00	3	0	0	0	3	0
.....................................TTCATCTGAGCTTGTAAGTACCTACA....................................................................................................	26	1	3.00	3	0	2	0	1	0
.............TTGTCCAAAAAGCGAGCAAGATACT.............................................................................................................................	25	1	2.00	2	0	2	0	0	0
......................................TCATCTGAGCTTGTAAGTACCTACA....................................................................................................	25	1	2.00	2	0	1	0	1	0
.......................................CATCTGAGCTTGTAAGTACCTACA....................................................................................................	24	1	2.00	2	0	2	0	0	0
..........................................................................................TATTTCATTGTGCTTCATTGCAGC.................................................	24	3	1.67	5	0	5	0	0	0
.......................................................................................TTATATTTCATTGTGCTTCATTGCAGC.................................................	27	3	1.33	4	0	3	0	1	0
.........................................TCTGAGCTTGTAAGTACCTACATATTT...............................................................................................	27	1	1.00	1	0	1	0	0	0
.........................................................................................ATATTTCATTGTGCTTCATTGCAGC.................................................	25	3	1.00	3	0	3	0	0	0
...........................................................................................................TTGCAGCTGAACAATTTTGATGAAGT..............................	26	1	1.00	1	0	0	0	1	0
......................................................................................................................CAATTTTGATGAAGTTGCTGACA......................	23	1	1.00	1	0	1	0	0	0
..............................................................................................TCATTGTGCTTCATTGCAGCTGAACA...........................................	26	1	1.00	1	0	1	0	0	0
............................................................................................................TGCAGCTGAACAATTTTGAAA..................................	21	1	1.00	1	0	0	0	1	0
.......................AGCGAGCAAGATACTTCATCTGAGC...................................................................................................................	25	1	1.00	1	0	0	0	1	0
..............TGTCCAAAAAGCGAGCAAGATACT.............................................................................................................................	24	1	1.00	1	0	1	0	0	0
............................................................................................................TGCAGCTGAACAATTTTGATGAAGTT.............................	26	1	1.00	1	0	1	0	0	0
......AAAGCGTTTGTCCAAAAAGCGAGCAAGA.................................................................................................................................	28	1	1.00	1	0	1	0	0	0
..........................................................................................TATTTCATTGTGCTTCATTGCAGCTGA..............................................	27	3	1.00	3	0	1	0	2	0
...............................................................................TTTCAAAATTATATTTCATTGTGCT...........................................................	25	1	1.00	1	0	0	0	1	0
...............................AGATACTTCATCTGAGCTTGTAAGTACC........................................................................................................	28	1	1.00	1	0	1	0	0	0
............................................................................................................TGCAGCTGAACAATTTTGATGAAGTTA............................	27	1	1.00	1	0	1	0	0	0
.......................................................................................TTATATTTCATTGTGCTTCATTGC....................................................	24	3	0.33	1	0	1	0	0	0
........................................................................................TATATTTCATTGTGCTTCATTGCAGCT................................................	27	3	0.33	1	0	1	0	0	0
...........................................................................................ATTTCATTGTGCTTCATTGCAGC.................................................	23	3	0.33	1	0	1	0	0	0
...............................................................................................CATTGTGCTTCATTGCAGC.................................................	19	3	0.33	1	0	1	0	0	0
..........................................................................................TATTTCATTGTGCTTCATTGCAGCT................................................	25	3	0.33	1	0	0	0	1	0
.............................................................................................TTCATTGTGCTTCATTGCAGCTG...............................................	23	3	0.33	1	0	1	0	0	0
..........................................................................................TATTTCATTGTGCTTCATTGCAGCA................................................	25	3	0.33	1	0	1	0	0	0
..........................................................................................TATTTCATTGTGCTTCATTGCAGCTG...............................................	26	3	0.33	1	0	1	0	0	0
.........................................................................................ATATTTCATTGTGCTTCATTGCAG..................................................	24	3	0.33	1	0	1	0	0	0
........................................................................................TATATTTCATTGTGCTTCAT.......................................................	20	3	0.33	1	0	0	0	1	0
..........................................................................................TATTTCATTGTGCTTCATTGCAG..................................................	23	3	0.33	1	0	0	0	1	0
..............................................................................................TCATTGTGCTTCATTGCAGCT................................................	21	3	0.33	1	0	1	0	0	0
............................................................................................................................................ATCTTAATGCAAGTGCCC.....	18	9	0.11	1	0	1	0	0	0

dwi_3517

scf2_1100000004943:15044205-15044267 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-3.4	-3.1	-3.0