ID:

dwi_3450

 Coordinate:

scf2_1100000004943:12336243-12336393 -

 Confidence:

Candidate

 Type:

Unknown

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

Flybase annnotation

exon [dwil_GLEANR_13332:1]; CDS [Dwil\GK12991-cds]; intron [Dwil\GK12991-in]

Repeatable elements

NameClassFamilyStrand
AT_richLow_complexityLow_complexity+

Sense Strand Reads

-------------------------------###################---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
ATTATTCTAAATTCGACTAAAATTCTGCTTAATGCAGAAGCAGCAGCAAGGTAATGGGAAATGTGACCGCATAAGCACTTGCACTATCTGGTCACACTTAAATATGTCTAGTTATGAGACAATGGCCAGAAAAATTGCCAGAAAGAGCTGAATGTCTTCTTCAATTAATATATAAAATATTTTATTGGATAACAAGTGCTTAAAATTATTTAACAATAAATTAATGTGGATTTCTCCTAAAAACCTCTTTT
**************************************************...(((((........)).)))(((((((((.....((((((((..........(((((.......))))).))))))))............((((........)))).((((((.(((((.....)))))..))))))...)))))))))************************************************** 		Read size # Mismatch Hit Count Total Norm Total M020
head
 M045
female body
 V118
embryo
 V119
head
.................................................................ACCGCATAACAACTTGGAC....................................................................................................................................................................... 19 3 20 28.45 569 296 273 0 0
..............................................................................................................................................................................AAATATTTTATGGGATAAGAA........................................................ 21 2 1 1.00 1 0 0 1 0
..TATTCTAAATTCGACTAAAAT.................................................................................................................................................................................................................................... 21 0 1 1.00 1 0 1 0 0
...............................................................................................................................................................................AATATTTTATGGGATAACA......................................................... 19 1 1 1.00 1 0 0 1 0
...........................................................................CAAATGCACTATCTGGTGACA........................................................................................................................................................... 21 3 5 0.20 1 0 0 0 1
.....................................................................................................................................................................................................................CAATCAATTAATGCGGAT.................... 18 2 11 0.18 2 2 0 0 0
...............................................................................................................................................................................AATATTTTATGGGATAAC.......................................................... 18 1 7 0.14 1 0 0 1 0
...............................................................................................................................................................................AATATTTTATGGGATAACCA........................................................ 20 2 7 0.14 1 0 0 1 0
............................................................................................................................................................................TTTAATATTTTATGGGATAACAA........................................................ 23 3 8 0.13 1 0 0 1 0
...............................................................................................................................................................................AATATTTTATGGGATAAGAAC....................................................... 21 3 17 0.12 2 0 0 2 0
.................................................................................................................................................................................TATTTTATGGGATAACAAC....................................................... 19 2 9 0.11 1 0 0 1 0
...............................................................................................................................................................................AATATTTTATGGGATAAAAA........................................................ 20 2 10 0.10 1 0 0 1 0
................................................................................................................................................................................ATATTTTATGGGATAAGAA........................................................ 19 2 15 0.07 1 0 0 1 0
....................................................................................................................................................................................................................ACAATCAATTAATGCGGATC................... 20 3 15 0.07 1 0 1 0 0
.............................................................................................................................................................................AAAATATTTTATGGGATAAA.......................................................... 20 2 19 0.05 1 0 0 1 0
.................................................................................................................................................................................TATTTTATTGGGGAACAA........................................................ 18 2 20 0.05 1 0 0 1 0
.................................................................ACCGCATAACGACTTGGAC....................................................................................................................................................................... 19 3 20 0.05 1 0 1 0 0

Anti-sense strand reads

TAATAAGATTTAAGCTGATTTTAAGACGAATTACGTCTTCGTCGTCGTTCCATTACCCTTTACACTGGCGTATTCGTGAACGTGATAGACCAGTGTGAATTTATACAGATCAATACTCTGTTACCGGTCTTTTTAACGGTCTTTCTCGACTTACAGAAGAAGTTAATTATATATTTTATAAAATAACCTATTGTTCACGAATTTTAATAAATTGTTATTTAATTACACCTAAAGAGGATTTTTGGAGAAAA
**************************************************...(((((........)).)))(((((((((.....((((((((..........(((((.......))))).))))))))............((((........)))).((((((.(((((.....)))))..))))))...)))))))))************************************************** 		Read size # Mismatch Hit Count Total Norm Total V118
embryo
 V119
head
 M020
head
.......ATTTAAGCTGATTTTAAGACGAGT............................................................................................................................................................................................................................ 24 1 1 1.00 1 1 0 0
.................................CGTCTTCGTCGTCGTTCCAT...................................................................................................................................................................................................... 20 0 1 1.00 1 0 1 0
..........TAAGCTGATTTTAAGACGAATTA.......................................................................................................................................................................................................................... 23 0 1 1.00 1 1 0 0
.....AGATTTAAGCTGATTTTA.................................................................................................................................................................................................................................... 18 0 1 1.00 1 0 0 1
.....................................................TACCCTTAACACTCGCCTAT.................................................................................................................................................................................. 20 3 15 0.07 1 0 1 0

Re-alignment of all predicted orthologs

Species Coordinate ID Alignment
droWil2 scf2_1100000004943:12336193-12336443 - dwi_3450 ATTATTCTA--------AATTCGACTAAAATTCTG------CTTAATGCAGAAGCAGCAGCAAGGTAATGGGAAATGTGACC-----------------GCATAAGCACTTGCACTATCTGGTCAC--------------------------------------------------------------------------------------------------------------------------------ACTTAAATATGTCTAGTTATGAGACAATGGCCAGAAAAATTGCCAGAAAGAGCTGAATGTCTTCTTCAATTAATATATAAAATATTTTATTGGATAACAAGTGCTTAAAATTATTTAACAATAAATTAATGTGGATTTCTCCTAAA-------------------------------------------------AACCTCTTTT
dp5 2:14249194-14249311 + ATTTTGCCA--------ATTTTTTCTTAAAT--------ATTTTTAAGCAGAAAGT-------------GGAGAACGTGAGCACTAGAGCAGTGTGACCGCGGAA-TTATAGTTAAAATATACGGTC-------------------------------------------------------------------------------------------------------------------------CGACCCACAGAAATATACCCA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droPer2 scaffold_0:6190806-6190928 - ATTTTGCCA--------ATTTTTTCTTAAAT--------ATTTTTAAGCAGAAAGT-------------GGAGAACGTGAGCACTAGAGCAGTGTGACCGCGGAA-TTATAGTTAAAATATACGGTC-------------------------------------------------------------------------------------------------------------------------CGACCCACCGAAATATACCAAAATAT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droAna3 scaffold_13340:9169278-9169482 + AAAAAATTA--------ATTTT------------CTAAATTTCTAAAGCAGCAAGT-------------GGAGTACGTGAGC------GCTGTGTGACCAGGCAG-CGAGAGTCAAAATATACCGTTAGAACGCGT---TT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------GAATCTGCGCTTCAAA----------------------------------------TATTTTTCTAATAAGCTTACTGGAACAT---ATAGAAAGAATAAACTCATCTCTAGCATTGTTATATTCATAACATA-AATATAAAAAATATTTT
droBip1 scf7180000396712:324167-324408 + ATTCTGGTAAAAAATGAATTTT------------CTAAATTTCTAAAGCAGCAAGT-------------GGAGTACGTGAGC------GCTGTGTGACCAGGCAT-TGGTGGTCAAACAATACTGAAATAAAGCGT---AT------------------------------------------------------------------------------ATATACTGGTATAAAATATACTGTTTTAATGGCGCTTTGAA--------------------------------------------------------------------------------------------------------TTATTTTCCAACATATAAGCTGGGATATATTATAGAAAGCGTAAATTATTCTTTAGAACTATAGTATTCATAAAATTACACAGTAAATACTTTTT
droKik1 scf7180000302461:1875418-1875643 - TTTCTACTG--------GCTTTGGCTGCT-----GCGGGAAATTAAGGCAGCAAGT-------------GGAGTACGTGCGT------GCAGTGTGACCAGCTGG-CGCTAGAAAAATCTGGCAGCGATAGCGAGCAGTATGACACATCACGCCAAACAGGCTAATATCAAAATAATTGAAATATCAGCAAACAATCGTGAAATCACAAAATGTCTAGCGGATTCTGAAATAAATTATTTTGTTTTT-------------------------------------------------------------------------------------------------------------------------------------------------------GC-------------------------------------------------CAATTTTATT

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droWil2
ATTATTCTA--------AATTCGACTAAAATTCTG------CTTAATGCAGAAGCAGCAGCAAGGTAATGGGAAATGTGACC-----------------GCATAAGCACTTGCACTATCTGGTCAC--------------------------------------------------------------------------------------------------------------------------------ACTTAAATATGTCTAGTTATGAGACAATGGCCAGAAAAATTGCCAGAAAGAGCTGAATGTCTTCTTCAATTAATATATAAAATATTTTATTGGATAACAAGTGCTTAAAATTATTTAACAATAAATTAATGTGGATTTCTCCTAAA-------------------------------------------------AACCTCTTTTSizeHit CountTotal NormTotalM020
Head		M045
Female-body		V117
Male-body		V118
Embryo		V119
Head
..TATTCTA--------AATTCGACTAAAAT............................................................................................................................................................................................................................................................................................................................................................................................................................................2111.00101000
dp5
ATTTTGCCA--------ATTTTTTCTTAAAT--------ATTTTTAAGCAGAAAGT-------------GGAGAACGTGAGCACTAGAGCAGTGTGACCGCGGAA-TTATAGTTAAAATATACGGTC-------------------------------------------------------------------------------------------------------------------------CGACCCACAGAAATATACCCA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SizeHit CountTotal NormTotalM022
Male-body		M040
Female-body		M059
Embryo		M062
Head		V043
Embryo		V051
Head		V112
Male-body		SRR902010
Ovary		SRR902011
Testis		SRR902012
CNS imaginal disc
No Reads
droPer2
ATTTTGCCA--------ATTTTTTCTTAAAT--------ATTTTTAAGCAGAAAGT-------------GGAGAACGTGAGCACTAGAGCAGTGTGACCGCGGAA-TTATAGTTAAAATATACGGTC-------------------------------------------------------------------------------------------------------------------------CGACCCACCGAAATATACCAAAATAT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SizeHit CountTotal NormTotalM021
Embryo		M042
Female-body		V042
Embryo		V050
Head		V057
Head		V111
Male-body
.............................................AAGCAGAAAGT-------------GGAGAAC...............................................................................................................................................................................................................................................................................................................................................................................................1811.001010000
droAna3
AAAAAATTA--------ATTTT------------CTAAATTTCTAAAGCAGCAAGT-------------GGAGTACGTGAGC------GCTGTGTGACCAGGCAG-CGAGAGTCAAAATATACCGTTAGAACGCGT---TT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------GAATCTGCGCTTCAAA----------------------------------------TATTTTTCTAATAAGCTTACTGGAACAT---ATAGAAAGAATAAACTCATCTCTAGCATTGTTATATTCATAACATA-AATATAAAAAATATTTTSizeHit CountTotal NormTotalM044
Female-body		M058
Embryo		V039
Embryo		V055
Head		V105
Male-body		V106
Head
No Reads
droBip1
ATTCTGGTAAAAAATGAATTTT------------CTAAATTTCTAAAGCAGCAAGT-------------GGAGTACGTGAGC------GCTGTGTGACCAGGCAT-TGGTGGTCAAACAATACTGAAATAAAGCGT---AT------------------------------------------------------------------------------ATATACTGGTATAAAATATACTGTTTTAATGGCGCTTTGAA--------------------------------------------------------------------------------------------------------TTATTTTCCAACATATAAGCTGGGATATATTATAGAAAGCGTAAATTATTCTTTAGAACTATAGTATTCATAAAATTACACAGTAAATACTTTTTSizeHit CountTotal NormTotal
No Reads
droKik1
TTTCTACTG--------GCTTTGGCTGCT-----GCGGGAAATTAAGGCAGCAAGT-------------GGAGTACGTGCGT------GCAGTGTGACCAGCTGG-CGCTAGAAAAATCTGGCAGCGATAGCGAGCAGTATGACACATCACGCCAAACAGGCTAATATCAAAATAATTGAAATATCAGCAAACAATCGTGAAATCACAAAATGTCTAGCGGATTCTGAAATAAATTATTTTGTTTTT-------------------------------------------------------------------------------------------------------------------------------------------------------GC-------------------------------------------------CAATTTTATTSizeHit CountTotal NormTotal
No Reads

Generated: 05/18/2015 at 07:05 AM