dwi_3359

##################################################--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- TTCCCGGTATGGACAAATTGGAGAGCGTTTATATCTTTATTTGGTCTTGGGTATGTAAGCCTGCGAACCCAGTCGAATGTTTCAATGCTCCAGCCATTCGTGAAGCAGTCTTGAGGGACCTCTATGGGTTGATTACCGTTGCTGACATAGGACAAATCGATCCAAAGCTATCGAAGCTACAAATCTCAGATCTGTGGAACTCTTTAATCAACGGCAACATCATCGATAAAATGGGAAAAGTTAAAGTGTAC **************************************************.............((((((........((((((((((.((........)).))))...))))))........))))))...********************************************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V117 male body	M045 female body	M020 head	V118 embryo	V119 head
.............................................................................................................CTTGAGGGACCTCTATGGGTTGAT......................................................................................................................	24	0	1	4.00	4	4	0	0	0	0
.................................................................................................................................................................................................................................ATAAAATGGGAAAAGTTAAAGT....	22	0	1	3.00	3	3	0	0	0	0
..............................ATATCTTTATTTGGTCTTGGGT.......................................................................................................................................................................................................	22	0	1	2.00	2	0	0	2	0	0
................................................................................................................................................................................................................................GATAAAATGGGAAAAGTTAAAGT....	23	0	1	2.00	2	1	1	0	0	0
....................................................ATGTAAGCCTGCGAACCCAGTCGAAT.............................................................................................................................................................................	26	0	1	2.00	2	1	1	0	0	0
..CCCGGTATGGACAAATTGGAGAGC.................................................................................................................................................................................................................................	24	0	1	2.00	2	2	0	0	0	0
...............................................................................................................................................................................................................................CGATAAAATGGGAAAAGTTAAA......	22	0	1	1.00	1	1	0	0	0	0
.............................................................................................................CTTGAGGGACCTCTATGGGTTGA.......................................................................................................................	23	0	1	1.00	1	1	0	0	0	0
........................................................................................................................................................CAAATCGATCAAAAGCTATC...............................................................................	20	1	1	1.00	1	1	0	0	0	0
...................................................................................................................................................................................................................................AAAATGGGAAACGTTAAAGT....	20	1	1	1.00	1	1	0	0	0	0
................................................................................................................................................................................CTACAAATCTCAGATCTGT........................................................	19	0	1	1.00	1	1	0	0	0	0
..................................................................................................CGTGAAGCAGTCTTGAGG.......................................................................................................................................	18	0	1	1.00	1	0	1	0	0	0
................................................................................TTCAATGCTCCAGCCATTCGTGAAGCA................................................................................................................................................	27	0	1	1.00	1	1	0	0	0	0
..........................GTTTATATCTTTATTTGGTCTTGGG........................................................................................................................................................................................................	25	0	1	1.00	1	0	1	0	0	0
......................................................................................................................................................................................ATCTCAGATCTGTGGAACTCT................................................	21	0	1	1.00	1	1	0	0	0	0
...................................................................................................................................................................................................GGAACTCTTTAATCAACGGCAACA................................	24	0	1	1.00	1	0	1	0	0	0
.....................................................................................................................................................................................AATCTCAGATCTGTGGAACTCT................................................	22	0	1	1.00	1	1	0	0	0	0
.....GGTATGGACAAATTGGAG....................................................................................................................................................................................................................................	18	0	1	1.00	1	1	0	0	0	0
..............................................................................................CATTCGTGAAGCAGTCTTGA.........................................................................................................................................	20	0	1	1.00	1	1	0	0	0	0
...............................................................................................................................................................................................................................CGATAAAATGGGAAAAGTTAAAGT....	24	0	1	1.00	1	1	0	0	0	0
.....................................................................................................................................TACCGTTGCTGACATAGGACAAATCGATC.........................................................................................	29	0	1	1.00	1	0	0	1	0	0
..................................................................................................................................................................................................................................TAAAATGGGAAAAGTTAAAGT....	21	0	1	1.00	1	1	0	0	0	0
......................................................................................................................................................................................ATCTCAGATCTGTGGAACT..................................................	19	0	1	1.00	1	0	0	1	0	0
............................................................................................GCCATTCGTGAAGCAGTCTTGAGG.......................................................................................................................................	24	0	1	1.00	1	1	0	0	0	0
...............................................................................................ATTCGTGAAGCAGTCTTGAGGGACCTCT................................................................................................................................	28	0	1	1.00	1	1	0	0	0	0
........ATGGACAAATTGGAGAGCGTT..............................................................................................................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0
..........................................................................GAATGTTTCAATGCTCCAGCCATTC........................................................................................................................................................	25	0	1	1.00	1	0	1	0	0	0
..........................................................................................................AGTCTTGAGGGACCTCTATGGGTTG........................................................................................................................	25	0	1	1.00	1	1	0	0	0	0
.....................................................................................................................................TACCGTTGCTGACATAGGACAAATC.............................................................................................	25	0	1	1.00	1	1	0	0	0	0
.............................................................................................................CTTGAGGGACCTCTATGGGTTG........................................................................................................................	22	0	1	1.00	1	1	0	0	0	0
...................................................................................................................................................................................................................................AAAATGGGAAAAGTTAAAGT....	20	0	1	1.00	1	1	0	0	0	0
...................................................................................................GTGAAGCAGTCTTGAGGGAC....................................................................................................................................	20	0	1	1.00	1	1	0	0	0	0
.............................................................................................................CTTGAGGGACCTCTATGG............................................................................................................................	18	0	1	1.00	1	1	0	0	0	0
.........................................................AGCCTGCGAACCCAGTCG................................................................................................................................................................................	18	0	1	1.00	1	0	0	0	1	0
..............................................................................................CATTCGTGAAGCAGTCTTGAGGGACCTCT................................................................................................................................	29	0	1	1.00	1	1	0	0	0	0
.....................................................................................................GAAGCAGTCTTGAGGGACCTCTATGG............................................................................................................................	26	0	1	1.00	1	1	0	0	0	0
...............AATTGGAGAGCGTTTATAT.........................................................................................................................................................................................................................	19	0	1	1.00	1	0	1	0	0	0
........................................................................................................................................................................................CTCAGATCTGTGGAACTCTTTA.............................................	22	0	1	1.00	1	0	1	0	0	0
................................................................................................................................................................................................................CAACGGCAACATCATCGATAAAATGGGA...............	28	0	1	1.00	1	1	0	0	0	0
.......................................................................................................AGCAGTCTTGAGGGACCTCTATGG............................................................................................................................	24	0	1	1.00	1	1	0	0	0	0
..........................GTTTATATCTTTATTTGGTCTTGGGT.......................................................................................................................................................................................................	26	0	1	1.00	1	1	0	0	0	0
..........................................................................................................................................................................................CAGATCTGTGGAACTCTTTAATCAAC.......................................	26	0	1	1.00	1	0	1	0	0	0
..................................................................................................CATGAAGCAGTCTTGCGGC......................................................................................................................................	19	3	12	0.08	1	0	1	0	0	0
...............................................................................................................................ATTGATTACAGTTGCTGA..........................................................................................................	18	2	20	0.05	1	0	0	0	0	1

##################################################---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
TTCCCGGTATGGACAAATTGGAGAGCGTTTATATCTTTATTTGGTCTTGGGTATGTAAGCCTGCGAACCCAGTCGAATGTTTCAATGCTCCAGCCATTCGTGAAGCAGTCTTGAGGGACCTCTATGGGTTGATTACCGTTGCTGACATAGGACAAATCGATCCAAAGCTATCGAAGCTACAAATCTCAGATCTGTGGAACTCTTTAATCAACGGCAACATCATCGATAAAATGGGAAAAGTTAAAGTGTAC
****************************************************.............((((((........((((((((((.((........)).))))...))))))........))))))...**********************************************************************************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

V117
male body

M045
female body

M020
head

V118
embryo

V119
head

.............................................................................................................CTTGAGGGACCTCTATGGGTTGAT......................................................................................................................

4.00

.................................................................................................................................................................................................................................ATAAAATGGGAAAAGTTAAAGT....

3.00

..............................ATATCTTTATTTGGTCTTGGGT.......................................................................................................................................................................................................

2.00

....................................................ATGTAAGCCTGCGAACCCAGTCGAAT.............................................................................................................................................................................

2.00

..CCCGGTATGGACAAATTGGAGAGC.................................................................................................................................................................................................................................

2.00

1.00

.............................................................................................................CTTGAGGGACCTCTATGGGTTGA.......................................................................................................................

1.00

........................................................................................................................................................CAAATCGATCAAAAGCTATC...............................................................................

1.00

................................................................................................................................................................................CTACAAATCTCAGATCTGT........................................................

1.00

..................................................................................................CGTGAAGCAGTCTTGAGG.......................................................................................................................................

1.00

................................................................................TTCAATGCTCCAGCCATTCGTGAAGCA................................................................................................................................................

1.00

..........................GTTTATATCTTTATTTGGTCTTGGG........................................................................................................................................................................................................

1.00

......................................................................................................................................................................................ATCTCAGATCTGTGGAACTCT................................................

1.00

...................................................................................................................................................................................................GGAACTCTTTAATCAACGGCAACA................................

1.00

.....................................................................................................................................................................................AATCTCAGATCTGTGGAACTCT................................................

1.00

.....GGTATGGACAAATTGGAG....................................................................................................................................................................................................................................

1.00

..............................................................................................CATTCGTGAAGCAGTCTTGA.........................................................................................................................................

1.00

.....................................................................................................................................TACCGTTGCTGACATAGGACAAATCGATC.........................................................................................

1.00

......................................................................................................................................................................................ATCTCAGATCTGTGGAACT..................................................

1.00

............................................................................................GCCATTCGTGAAGCAGTCTTGAGG.......................................................................................................................................

1.00

...............................................................................................ATTCGTGAAGCAGTCTTGAGGGACCTCT................................................................................................................................

1.00

........ATGGACAAATTGGAGAGCGTT..............................................................................................................................................................................................................................

1.00

..........................................................................GAATGTTTCAATGCTCCAGCCATTC........................................................................................................................................................

1.00

..........................................................................................................AGTCTTGAGGGACCTCTATGGGTTG........................................................................................................................

1.00

.....................................................................................................................................TACCGTTGCTGACATAGGACAAATC.............................................................................................

1.00

.............................................................................................................CTTGAGGGACCTCTATGGGTTG........................................................................................................................

1.00

...................................................................................................GTGAAGCAGTCTTGAGGGAC....................................................................................................................................

1.00

.............................................................................................................CTTGAGGGACCTCTATGG............................................................................................................................

1.00

.........................................................AGCCTGCGAACCCAGTCG................................................................................................................................................................................

1.00

..............................................................................................CATTCGTGAAGCAGTCTTGAGGGACCTCT................................................................................................................................

1.00

.....................................................................................................GAAGCAGTCTTGAGGGACCTCTATGG............................................................................................................................

1.00

...............AATTGGAGAGCGTTTATAT.........................................................................................................................................................................................................................

1.00

........................................................................................................................................................................................CTCAGATCTGTGGAACTCTTTA.............................................

1.00

.......................................................................................................AGCAGTCTTGAGGGACCTCTATGG............................................................................................................................

1.00

..........................GTTTATATCTTTATTTGGTCTTGGGT.......................................................................................................................................................................................................

1.00

..........................................................................................................................................................................................CAGATCTGTGGAACTCTTTAATCAAC.......................................

1.00

..................................................................................................CATGAAGCAGTCTTGCGGC......................................................................................................................................

0.08

...............................................................................................................................ATTGATTACAGTTGCTGA..........................................................................................................

0.05

AAGGGCCATACCTGTTTAACCTCTCGCAAATATAGAAATAAACCAGAACCCATACATTCGGACGCTTGGGTCAGCTTACAAAGTTACGAGGTCGGTAAGCACTTCGTCAGAACTCCCTGGAGATACCCAACTAATGGCAACGACTGTATCCTGTTTAGCTAGGTTTCGATAGCTTCGATGTTTAGAGTCTAGACACCTTGAGAAATTAGTTGCCGTTGTAGTAGCTATTTTACCCTTTTCAATTTCACATG ********************************************************************************************************************.............((((((........((((((((((.((........)).))))...))))))........))))))...**************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M045 female body	V119 head
................................................................................AAGCTACGAAGTCGGTAAGGA......................................................................................................................................................	21	3	3	0.33	1	1	0
.............................................................................................................GAACTCCCGGGAGAAACTC...........................................................................................................................	19	3	8	0.13	1	1	0
............................................................................................................................ACGCAGCTAATTGCAACG.............................................................................................................	18	3	20	0.05	1	1	0
............................................AGAACCCCTCTATTCGGA.............................................................................................................................................................................................	18	3	20	0.05	1	0	1

AAGGGCCATACCTGTTTAACCTCTCGCAAATATAGAAATAAACCAGAACCCATACATTCGGACGCTTGGGTCAGCTTACAAAGTTACGAGGTCGGTAAGCACTTCGTCAGAACTCCCTGGAGATACCCAACTAATGGCAACGACTGTATCCTGTTTAGCTAGGTTTCGATAGCTTCGATGTTTAGAGTCTAGACACCTTGAGAAATTAGTTGCCGTTGTAGTAGCTATTTTACCCTTTTCAATTTCACATG
**********************************************************************************************************************.............((((((........((((((((((.((........)).))))...))))))........))))))...****************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M045
female body

V119
head

................................................................................AAGCTACGAAGTCGGTAAGGA......................................................................................................................................................

0.33

.............................................................................................................GAACTCCCGGGAGAAACTC...........................................................................................................................

0.13

............................................................................................................................ACGCAGCTAATTGCAACG.............................................................................................................

0.05

............................................AGAACCCCTCTATTCGGA.............................................................................................................................................................................................

0.05

Species	Coordinate	ID	Alignment
droWil2	scf2_1100000004943:8000002-8000252 +	dwi_3359	TTCCCGGTATGGACAAATTGGAGAGCGTTTATATCTTTATTTGGTCTTGGGTATGTAAGCCTGCGAACCCAGTCGAATGTTTCAATGCTCCAGCCATTCGTGAAGCAGTCTTGAGGGACCTCTATGGGTTGATTACCGTTGCTGACATAGGACAAATCGATCCAAAGCTATCGAAGCTACAAATCTCAGATCTGTGGAACTCTTTAATCAACGGCAACATCATCGATAAAATGGGAAAAGTTAAAGTGTAC

Species

Coordinate

Alignment

droWil2

scf2_1100000004943:8000002-8000252 +

dwi_3359

TTCCCGGTATGGACAAATTGGAGAGCGTTTATATCTTTATTTGGTCTTGGGTATGTAAGCCTGCGAACCCAGTCGAATGTTTCAATGCTCCAGCCATTCGTGAAGCAGTCTTGAGGGACCTCTATGGGTTGATTACCGTTGCTGACATAGGACAAATCGATCCAAAGCTATCGAAGCTACAAATCTCAGATCTGTGGAACTCTTTAATCAACGGCAACATCATCGATAAAATGGGAAAAGTTAAAGTGTAC

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droWil2

TTCCCGGTATGGACAAATTGGAGAGCGTTTATATCTTTATTTGGTCTTGGGTATGTAAGCCTGCGAACCCAGTCGAATGTTTCAATGCTCCAGCCATTCGTGAAGCAGTCTTGAGGGACCTCTATGGGTTGATTACCGTTGCTGACATAGGACAAATCGATCCAAAGCTATCGAAGCTACAAATCTCAGATCTGTGGAACTCTTTAATCAACGGCAACATCATCGATAAAATGGGAAAAGTTAAAGTGTAC	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
.............................................................................................................CTTGAGGGACCTCTATGGGTTGAT......................................................................................................................	24	1	4.00	4	0	0	4	0	0
.................................................................................................................................................................................................................................ATAAAATGGGAAAAGTTAAAGT....	22	1	3.00	3	0	0	3	0	0
..............................ATATCTTTATTTGGTCTTGGGT.......................................................................................................................................................................................................	22	1	2.00	2	2	0	0	0	0
................................................................................................................................................................................................................................GATAAAATGGGAAAAGTTAAAGT....	23	1	2.00	2	0	1	1	0	0
....................................................ATGTAAGCCTGCGAACCCAGTCGAAT.............................................................................................................................................................................	26	1	2.00	2	0	1	1	0	0
..CCCGGTATGGACAAATTGGAGAGC.................................................................................................................................................................................................................................	24	1	2.00	2	0	0	2	0	0
...............................................................................................................................................................................................................................CGATAAAATGGGAAAAGTTAAA......	22	1	1.00	1	0	0	1	0	0
.............................................................................................................CTTGAGGGACCTCTATGGGTTGA.......................................................................................................................	23	1	1.00	1	0	0	1	0	0
................................................................................................................................................................................CTACAAATCTCAGATCTGT........................................................	19	1	1.00	1	0	0	1	0	0
..................................................................................................CGTGAAGCAGTCTTGAGG.......................................................................................................................................	18	1	1.00	1	0	1	0	0	0
................................................................................TTCAATGCTCCAGCCATTCGTGAAGCA................................................................................................................................................	27	1	1.00	1	0	0	1	0	0
..........................GTTTATATCTTTATTTGGTCTTGGG........................................................................................................................................................................................................	25	1	1.00	1	0	1	0	0	0
......................................................................................................................................................................................ATCTCAGATCTGTGGAACTCT................................................	21	1	1.00	1	0	0	1	0	0
...................................................................................................................................................................................................GGAACTCTTTAATCAACGGCAACA................................	24	1	1.00	1	0	1	0	0	0
.....................................................................................................................................................................................AATCTCAGATCTGTGGAACTCT................................................	22	1	1.00	1	0	0	1	0	0
.....GGTATGGACAAATTGGAG....................................................................................................................................................................................................................................	18	1	1.00	1	0	0	1	0	0
..............................................................................................CATTCGTGAAGCAGTCTTGA.........................................................................................................................................	20	1	1.00	1	0	0	1	0	0
...............................................................................................................................................................................................................................CGATAAAATGGGAAAAGTTAAAGT....	24	1	1.00	1	0	0	1	0	0
.....................................................................................................................................TACCGTTGCTGACATAGGACAAATCGATC.........................................................................................	29	1	1.00	1	1	0	0	0	0
..................................................................................................................................................................................................................................TAAAATGGGAAAAGTTAAAGT....	21	1	1.00	1	0	0	1	0	0
......................................................................................................................................................................................ATCTCAGATCTGTGGAACT..................................................	19	1	1.00	1	1	0	0	0	0
............................................................................................GCCATTCGTGAAGCAGTCTTGAGG.......................................................................................................................................	24	1	1.00	1	0	0	1	0	0
...............................................................................................ATTCGTGAAGCAGTCTTGAGGGACCTCT................................................................................................................................	28	1	1.00	1	0	0	1	0	0
........ATGGACAAATTGGAGAGCGTT..............................................................................................................................................................................................................................	21	1	1.00	1	0	0	1	0	0
..........................................................................GAATGTTTCAATGCTCCAGCCATTC........................................................................................................................................................	25	1	1.00	1	0	1	0	0	0
..........................................................................................................AGTCTTGAGGGACCTCTATGGGTTG........................................................................................................................	25	1	1.00	1	0	0	1	0	0
.....................................................................................................................................TACCGTTGCTGACATAGGACAAATC.............................................................................................	25	1	1.00	1	0	0	1	0	0
.............................................................................................................CTTGAGGGACCTCTATGGGTTG........................................................................................................................	22	1	1.00	1	0	0	1	0	0
...................................................................................................................................................................................................................................AAAATGGGAAAAGTTAAAGT....	20	1	1.00	1	0	0	1	0	0
...................................................................................................GTGAAGCAGTCTTGAGGGAC....................................................................................................................................	20	1	1.00	1	0	0	1	0	0
.............................................................................................................CTTGAGGGACCTCTATGG............................................................................................................................	18	1	1.00	1	0	0	1	0	0
.........................................................AGCCTGCGAACCCAGTCG................................................................................................................................................................................	18	1	1.00	1	0	0	0	1	0
..............................................................................................CATTCGTGAAGCAGTCTTGAGGGACCTCT................................................................................................................................	29	1	1.00	1	0	0	1	0	0
.....................................................................................................GAAGCAGTCTTGAGGGACCTCTATGG............................................................................................................................	26	1	1.00	1	0	0	1	0	0
...............AATTGGAGAGCGTTTATAT.........................................................................................................................................................................................................................	19	1	1.00	1	0	1	0	0	0
........................................................................................................................................................................................CTCAGATCTGTGGAACTCTTTA.............................................	22	1	1.00	1	0	1	0	0	0
................................................................................................................................................................................................................CAACGGCAACATCATCGATAAAATGGGA...............	28	1	1.00	1	0	0	1	0	0
.......................................................................................................AGCAGTCTTGAGGGACCTCTATGG............................................................................................................................	24	1	1.00	1	0	0	1	0	0
..........................GTTTATATCTTTATTTGGTCTTGGGT.......................................................................................................................................................................................................	26	1	1.00	1	0	0	1	0	0
..........................................................................................................................................................................................CAGATCTGTGGAACTCTTTAATCAAC.......................................	26	1	1.00	1	0	1	0	0	0

dwi_3359

scf2_1100000004943:8000052-8000202 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-16.4	-15.9	-15.7