ID:

dwi_2912

 Coordinate:

scf2_1100000004909:12134366-12134516 -

 Confidence:

Candidate

 Type:

Unknown

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

Flybase annnotation

CDS [Dwil\GK24914-cds]; exon [dwil_GLEANR_9086:5]; intron [Dwil\GK24914-in]; Antisense to intron [Dwil\GK10242-in]

No Repeatable elements found

Sense Strand Reads

##################################################---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
TTGCACCGATGGTTCCGACGAAGATCCTCAAATGTGTGCAGCCCACCGAGGTTTGTTCTGCTTGTTGGGGAAAGAAAAATCGGGTTCAATTCAACATACACAACATATATACACATACATTCATATTAAGTCTATTCGTGGAAGGGAATGCAAAACGAAACGAATTTGAAAGAAACAACAAAAATTTTCGGATCAAAGTTACGATTGCAATCCCTGAAAGAGGAGGGGAAAAATGTCAAAAGCTTTTGTGC
**************************************************((.((((.((..(((((((....(((........)))..)))))))..)).)))).)).........(((((........((((....))))...)))))......((..((((......................))))..)).......************************************************** 		Read size # Mismatch Hit Count Total Norm Total V118
embryo
 M045
female body
 V117
male body
............................CAAATGTGTGCAGCCCACCGAGAGA...................................................................................................................................................................................................... 25 3 1 1.00 1 1 0 0
..................CGAAGATCCTCAAATGTGTGCAGCC................................................................................................................................................................................................................ 25 0 1 1.00 1 1 0 0
...CACCGATGGTTCCGACGAAGATCCT............................................................................................................................................................................................................................... 25 0 1 1.00 1 1 0 0
...........GTTCCGACGAAGATCCTC.............................................................................................................................................................................................................................. 18 0 1 1.00 1 1 0 0
...........................................................................CAGATCGGGTTCAATTCC.............................................................................................................................................................. 18 3 20 0.05 1 0 0 1
...................................................................................................................................................................................................................................GAAAGATGTCAAAAGCTGGT.... 20 3 20 0.05 1 0 1 0

Anti-sense strand reads

AACGTGGCTACCAAGGCTGCTTCTAGGAGTTTACACACGTCGGGTGGCTCCAAACAAGACGAACAACCCCTTTCTTTTTAGCCCAAGTTAAGTTGTATGTGTTGTATATATGTGTATGTAAGTATAATTCAGATAAGCACCTTCCCTTACGTTTTGCTTTGCTTAAACTTTCTTTGTTGTTTTTAAAAGCCTAGTTTCAATGCTAACGTTAGGGACTTTCTCCTCCCCTTTTTACAGTTTTCGAAAACACG
**************************************************((.((((.((..(((((((....(((........)))..)))))))..)).)))).)).........(((((........((((....))))...)))))......((..((((......................))))..)).......************************************************** 		Read size # Mismatch Hit Count Total Norm Total M045
female body
 V117
male body
.....................................................................................................................................................................................TTTAGAGGCCTAGTTTCGA................................................... 19 3 16 0.94 15 14 1
..................................................................................................................................................................................GTCTTTAGAGGCCTAGTTTC..................................................... 20 3 7 0.14 1 1 0

Re-alignment of all predicted orthologs

Species Coordinate ID Alignment
droWil2 scf2_1100000004909:12134316-12134566 - dwi_2912 TTGCACCGATGGTTCCGACGAAGATCCTCAAATGTGTGCAGCCCACCGAGGTTTGTTCTGCTTGTTGGGGAAAGAAAAATCGGGTTCAATTCAACATACACA-ACATATATACACATACATTCATATTAAGTCTATTCG-TGGAAGGGAATGCAAAACGAAACGAATTTGAAAG--AAACAACAAAAATTTTCGGATCAAAGTTACGATTGCAA---------TCCCTGAAAGA-GGAGGGGAAAAATGTC---------------AAAAGC--TT-TT---GTGC
droVir3 scaffold_13049:23177211-23177283 - TCAGCA------------------------------------------------------------------------------------------TATATA-ATATATATATATATATATATATCTGAATACAGTTAA-TGTCAGGGCAATTCTAA---------------A-------------------------------------------------------------------------------------------------------GAA---ATCG
droMoj3 scaffold_6500:10387981-10388041 - TGTTGCA----CAAAGAGTATT----------------------------------------------------------------CCATACAACATATAAA-ATAAATATAAATATATATATATATA------------------------------------------------------------------------------------------------------------------------------------------------------------AA
droGri2 scaffold_15110:1748823-1748979 + AATCAAACGTA-----------------------------------------------------------------------------------CACACACACACACACACACACACACACACACACAGAGAACACGCACTAGAACTAAATGCA---TCAAACGAACTTCAAAGGCAAACG-------------------------GACGTC--GTAGG-TA------GAAAAAGAA-GAGAAAAACTAAAAACAAAAACAAAAACA-----AAAACTT---GTAT
droPer2 scaffold_8:3367320-3367327 + TTTT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGGT
droAna3 scaffold_12916:5269599-5269701 - GGATATAAAAGTAAAAAAAAAAGTTCTT------------GGGTATTA--TTCAT-------------------------------------------------------------------------------------------------------------------------------------------------------ATTTTCTTTTAAAAGTTTCCTTAAAAAA-TAATCTTAAAAATAT-----------------ATAATATTTCTCTCAGTGT
droEle1 scf7180000490478:9888-9959 - TGATGATGATGATGATGATGATGATTAT------------GATTACCG--ACTTT------------------------------------------------------------------------------TTCCAC-TTTTTGGGAAGTAGCCA---------------A-------------------------------------------------------------------------------------------------------GTC---GTGT
droSim2 2r:6193078-6193174 + CAAAAAAGGACCAAAGGGAAAGCAATAA------------C-AAATTG--ATTAA-------------------------------------------------------------------------------------------------------------------------------------------------------AACTTCGAGTAGA-CG------GAAGAAAGG-AAGAAAAGATGAA---------------GAAAGCAAATCTTTCATTTA
droEre2 scaffold_4929:8952938-8953035 - CAAAAAAGGACCAAAGGGAAAGCAATAA------------C-AAATTG--ATTAA-------------------------------------------------------------------------------------------------------------------------------------------------------TACTTCGAGTAGA-CG------GAAGAAAGGAAAGAAAAGATGAA---------------GAAAGCAAATCTTTCATTTA

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droWil2
TTGCACCGATGGTTCCGACGAAGATCCTCAAATGTGTGCAGCCCACCGAGGTTTGTTCTGCTTGTTGGGGAAAGAAAAATCGGGTTCAATTCAACATACACA-ACATATATACACATACATTCATATTAAGTCTATTCG-TGGAAGGGAATGCAAAACGAAACGAATTTGAAAG--AAACAACAAAAATTTTCGGATCAAAGTTACGATTGCAA---------TCCCTGAAAGA-GGAGGGGAAAAATGTC---------------AAAAGC--TT-TT---GTGCSizeHit CountTotal NormTotalM020
Head		M045
Female-body		V117
Male-body		V118
Embryo		V119
Head
............................CAAATGTGTGCAGCCCACCGAGAGA.........................................................................................................................................................................................................................................2511.00100010
..................CGAAGATCCTCAAATGTGTGCAGCC...................................................................................................................................................................................................................................................2511.00100010
...CACCGATGGTTCCGACGAAGATCCT..................................................................................................................................................................................................................................................................2511.00100010
...........GTTCCGACGAAGATCCTC.................................................................................................................................................................................................................................................................1811.00100010
droVir3
TCAGCA------------------------------------------------------------------------------------------TATATA-ATATATATATATATATATATATCTGAATACAGTTAA-TGTCAGGGCAATTCTAA---------------A-------------------------------------------------------------------------------------------------------GAA---ATCGSizeHit CountTotal NormTotalM027
Male-body		M028
Head		M047
Female-body		M061
Embryo		SRR060654
Ovary		SRR060655
Testis		SRR060656
Ovary		SRR060657
Testis		SRR060658
Ovary		SRR060659
Testis		SRR060660
Ovary		SRR060661
Embryo		SRR060662
Embryo		SRR060663
Embryo		SRR060664
Male-body		SRR060665
Female-body		SRR060666
Male-body		SRR060667
Female-body		SRR060668
Male-body		SRR060669
Female-body		SRR060670
Testis		SRR060671
Male-body		SRR060672
Female-body		SRR060673
Ovary		SRR060674
Ovary		SRR060675
Ovary		SRR060676
Ovary		SRR060677
Ovary		SRR060678
Testis		SRR060679
Testis		SRR060680
Testis		SRR060681
Testis		SRR060682
Embryo		SRR060683
Testis		SRR060684
Embryo		SRR060685
Embryo		SRR060686
Embryo		SRR060687
Embryo		SRR060688
Ovary		SRR060689
Testis		SRR1106712
embryo_0-2h		SRR1106713
embryo_0-2h		SRR1106714
embryo_2-4h		SRR1106715
embryo_4-6h		SRR1106716
embryo_4-6h		SRR1106717
embryo_6-8h		SRR1106718
embryo_6-8h		SRR1106719
embryo_8-10h		SRR1106720
embryo_8-10h		SRR1106721
embryo_10-12h		SRR1106722
embryo_10-12h		SRR1106723
embryo_12-14h		SRR1106724
embryo_12-14h		SRR1106725
embryo_14-16h		SRR1106726
embryo_14-16h		SRR1106727
larvae		SRR1106728
larvae		SRR1106729
mixed whole adult body		SRR1106730
embryo_16-30h		V047
Embryo		V053
Head		V116
Male-body		GSM1528803
follicle cells
No Reads
droMoj3
TGTTGCA----CAAAGAGTATT----------------------------------------------------------------CCATACAACATATAAA-ATAAATATAAATATATATATATATA------------------------------------------------------------------------------------------------------------------------------------------------------------AASizeHit CountTotal NormTotalM046
Female-body		V041
Embryo		V049
Head		V056
Head		M060
Embryo		V110
Male-body
No Reads
droGri2
AATCAAACGTA-----------------------------------------------------------------------------------CACACACACACACACACACACACACACACACACAGAGAACACGCACTAGAACTAAATGCA---TCAAACGAACTTCAAAGGCAAACG-------------------------GACGTC--GTAGG-TA------GAAAAAGAA-GAGAAAAACTAAAAACAAAAACAAAAACA-----AAAACTT---GTATSizeHit CountTotal NormTotalM041
Female-body		V109
Male-body
.......CGTA-----------------------------------------------------------------------------------CACACACACACACACA................................................................................................................................................................................20200.05110
droPer2
TTTT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGGTSizeHit CountTotal NormTotalM021
Embryo		M042
Female-body		V042
Embryo		V050
Head		V057
Head		V111
Male-body
No Reads
droAna3
GGATATAAAAGTAAAAAAAAAAGTTCTT------------GGGTATTA--TTCAT-------------------------------------------------------------------------------------------------------------------------------------------------------ATTTTCTTTTAAAAGTTTCCTTAAAAAA-TAATCTTAAAAATAT-----------------ATAATATTTCTCTCAGTGTSizeHit CountTotal NormTotalM044
Female-body		M058
Embryo		V039
Embryo		V055
Head		V105
Male-body		V106
Head
No Reads
droEle1
TGATGATGATGATGATGATGATGATTAT------------GATTACCG--ACTTT------------------------------------------------------------------------------TTCCAC-TTTTTGGGAAGTAGCCA---------------A-------------------------------------------------------------------------------------------------------GTC---GTGTSizeHit CountTotal NormTotal
No Reads
droSim2
CAAAAAAGGACCAAAGGGAAAGCAATAA------------C-AAATTG--ATTAA-------------------------------------------------------------------------------------------------------------------------------------------------------AACTTCGAGTAGA-CG------GAAGAAAGG-AAGAAAAGATGAA---------------GAAAGCAAATCTTTCATTTASizeHit CountTotal NormTotalM023
Head		M024
Male-body		M025
Embryo		M053
Female-body		O001
Testis		O002
Head		SRR553485
Ovary		SRR553486
Ovary		SRR553487
Ovary		SRR553488
Ovary		SRR618934
Ovary		V044
Embryo		SRR902008
Ovary		SRR902009
Testis		SRR1275487
Male larvae		SRR1275485
Male prepupae		SRR1275483
Male prepupae
No Reads
droEre2
CAAAAAAGGACCAAAGGGAAAGCAATAA------------C-AAATTG--ATTAA-------------------------------------------------------------------------------------------------------------------------------------------------------TACTTCGAGTAGA-CG------GAAGAAAGGAAAGAAAAGATGAA---------------GAAAGCAAATCTTTCATTTASizeHit CountTotal NormTotalM055
Female-body		M057
Embryo		V060
Head		V040
Embryo		V107
Male-body		V108
Head		GSM1528801
follicle cells
No Reads

Generated: 05/18/2015 at 05:33 AM