ID:

dwi_2465

 Coordinate:

scf2_1100000004902:3392682-3392832 -

 Confidence:

Candidate

 Type:

Unknown

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

Flybase annnotation

exon [dwil_GLEANR_11558:2]; CDS [Dwil\kl-5-cds]; intron [Dwil\kl-5-in]

No Repeatable elements found

Sense Strand Reads

##################################################---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
GTCTGAAGCAAGAGAAATGGACCAAAATGATAACAACAAACGAGTACAAGGTAAGACTTCATCATCATCAGCAGCAGCAGCATCTTCAGTTCAATCCTGATGTCCAGGAGCCTGTTTGGCATCCTGCTCTTTTGACACTCTTCTGTCCTGTCACAAATTTTTAATTTGCATAATTTGCTGCCGTTTGCCGTTGCCAGGCCTGCAAATCTGCTAAACGCGTTGGCCTTTTTATTTAATTTTTTTTTTTTTTT
**************************************************...................(((.(((((((((((..............))))).((((((((.....))).))))).......((((......))))......((((((...)))))).......)))))).))).(((.......)))..************************************************** 		Read size # Mismatch Hit Count Total Norm Total M045
female body
.......................................................................................................................................................................................................GTTCAAATCTGCTAAACG.................................. 18 2 7 0.14 1 1
....................................................................CAGCAGCACCGGCATCTTCA................................................................................................................................................................... 20 2 11 0.09 1 1

Anti-sense strand reads

CAGACTTCGTTCTCTTTACCTGGTTTTACTATTGTTGTTTGCTCATGTTCCATTCTGAAGTAGTAGTAGTCGTCGTCGTCGTAGAAGTCAAGTTAGGACTACAGGTCCTCGGACAAACCGTAGGACGAGAAAACTGTGAGAAGACAGGACAGTGTTTAAAAATTAAACGTATTAAACGACGGCAAACGGCAACGGTCCGGACGTTTAGACGATTTGCGCAACCGGAAAAATAAATTAAAAAAAAAAAAAAA
**************************************************...................(((.(((((((((((..............))))).((((((((.....))).))))).......((((......))))......((((((...)))))).......)))))).))).(((.......)))..************************************************** 		Read size # Mismatch Hit Count Total Norm Total M045
female body
 M020
head
 V117
male body
......................................................................................................................................................................................CCAACGGCAACCGTCCGGTCG................................................ 21 3 3 0.33 1 1 0 0
...........................................................................................................................................................................TTGAACGACTGCAAACGGC............................................................. 19 2 3 0.33 1 1 0 0
..........................................TCATGATCCATTATGACGTA............................................................................................................................................................................................. 20 3 5 0.20 1 1 0 0
..........................................................................................................CCTCGGAGAAACCAAAGGAC............................................................................................................................. 20 3 5 0.20 1 0 1 0
.............................................................................................................CGGACACACCGTCGTACGAG.......................................................................................................................... 20 3 6 0.17 1 1 0 0
........................................................................................................GGCGTCGGACAAACTGTAGG............................................................................................................................... 20 3 8 0.13 1 1 0 0
........................................................GAAGTAGCAGTAGGCGTCGT............................................................................................................................................................................... 20 2 19 0.05 1 0 0 1

Re-alignment of all predicted orthologs

Species Coordinate ID Alignment
droWil2 scf2_1100000004902:3392632-3392882 - dwi_2465 GTCTGAAGCAAGAGAAATGGACCAAAATGATAACAACAAACGAGTACAAGGTAAGACTTCATCATCATCAGCAGCAGCAGCATCTTCAGTTCAATCCTGATGTCCAGGAGCCTGTTTGGCATCCTGCTCTTTTGACACTCTTCTGTCCTGTCACAAATTTTTAATTTGCATAATTTGCTGCCGTTTGCCGTTGCCAGGCCTGCAAATCTGCTA-AACGCGTTGGCCTTTTTATTTAATTTTTTTTTTTTTTT
droMoj3 scaffold_4673:348-403 + GACTAAAATTAGATAAATGGACCAAGATGATAACAACCAATGAATACAAGGTAAG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------A
droGri2 scaffold_14849:47343-47397 + GACTAAAGCTAGAAAAATGGATTAGGTTGACGACAATTAATGAATATAAGGTAAG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
dp5 2:8818859-8818913 - GCCTAAAGCTGGAGAAATGGACCAAGCTGATAACCACCAACGAGTACAAGGTAAG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droPer2 scaffold_0:2631586-2631640 - GACTAAAGCTGGAGAAATGGACCAAGCTGATAACCACCAACGAGTACAAGGTAAG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droAna3 scaffold_13340:19536180-19536248 + CACA---------------------------------------------------------------------------------------------------------------------------------------------------------TTTACTGCCTGAATAATTTGCCACAGGTCCCA------------------GTGGTA-AACGAGTTGTCCTTTTT-TATAA-TTTA---------T
droBip1 scf7180000396413:2850160-2850255 - TCTGAAGCACTTTTACCTTGAT--------------------------------------------------------------------------------------------------------------------------TTC----GGCACATTTACTGTCTGAATAATTTGCCACAGCTCCCA------------------GTGGTA-AACGAGTTGTCCCTTTT-TTAAA-TTTA---------T
droKik1 scf7180000302744:5207-5273 + GCCTTAAACTTGAAAAGTGGACGAAAATGATAACAACCAACGAGTTCAAGGTAAG---------------------------------------------------------------------------------------------------------------------------------------------------------------------TTGTATCATT-----------A---------T
droEle1 scf7180000491261:1636518-1636588 - ATTGA------------------------------------------------------------------------------------------------------------------------------------------------------------CAGTTCGCATAATTGGCTGCTACTTTCG------------------CTGGTA-AACGAGTTGTCCCTTTT-TATAA-TTTATAATTT----
droBia1 scf7180000302075:1788602-1788687 + CTTCTACT----------------------------------------------------------------------------------------------------------------------------------------TTC----CACGCATTTTCAGTTTGCATAATTGGCCTCTGCCTCCG------------------GCAGTA-AACGAGTTGTCCCTTTT--ATAA-TTTGTAATTT----
droTak1 scf7180000415700:554266-554339 - CGAG---------------------------------------------------------------------------------------------------------------------------------------------------------ATTCCAGTTCGCATAATTGGCCGCTGCTTCCG------------------TTGGCAAAAAGAGTTGTCCTTTTT--ATAA-TTTATAATTT----
droEug1 scf7180000409770:626346-626416 - TTTGA-------------------------------------------------------------------------------------------------------------------------------------------------------------TTTTAGCATAATTGGCTGTTTCTTCCT------------------TTGTTA-AAAGAGTTGTCCCTTTTTTATAA-TTTTTAATTT----
droSim2 3r:22595965-22596044 - TTTG-------------------------------------------------------------------------------------------------------------------------------------------------------------CAGTTCGCATAATTGGCTAATGCTTCCT-TTTCGAG----------ATATTC-AGCAGGTTGTCCCTTTT--ATAA-TGTATAATTTAAAT
droSec2 scaffold_3704:348-402 + GTCTTAAGCTGGATAAGTGGACTAAAATGATAACAACCAACGAGTTTAGGGTGAG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droYak3 3R:23336928-23337008 + ATTTA------------------------------------------------------------------------------------------------------------------------------------------------------------CAGTTCGCATAATTGGCTGATGCTTCCT-TTTCGAG----------ATGTCG-AGCAGATTGTCCCTTTT--ATAA-TGTATAATTTAAAT
droEre2 scaffold_4820:4876583-4876662 + TTTA-------------------------------------------------------------------------------------------------------------------------------------------------------------CAGTTTGCATAATTGGCTGATGCTCCCT-TTTCGAG----------ATGTTG-AGCAGGTTGTCCCTTTT--ATAA-TGTATAATTAAAAT

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droWil2
GTCTGAAGCAAGAGAAATGGACCAAAATGATAACAACAAACGAGTACAAGGTAAGACTTCATCATCATCAGCAGCAGCAGCATCTTCAGTTCAATCCTGATGTCCAGGAGCCTGTTTGGCATCCTGCTCTTTTGACACTCTTCTGTCCTGTCACAAATTTTTAATTTGCATAATTTGCTGCCGTTTGCCGTTGCCAGGCCTGCAAATCTGCTA-AACGCGTTGGCCTTTTTATTTAATTTTTTTTTTTTTTTSizeHit CountTotal NormTotalM020
Head		M045
Female-body		V117
Male-body		V118
Embryo		V119
Head
No Reads
droMoj3
GACTAAAATTAGATAAATGGACCAAGATGATAACAACCAATGAATACAAGGTAAG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ASizeHit CountTotal NormTotalM046
Female-body		V041
Embryo		V049
Head		V056
Head		M060
Embryo		V110
Male-body
No Reads
droGri2
GACTAAAGCTAGAAAAATGGATTAGGTTGACGACAATTAATGAATATAAGGTAAG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SizeHit CountTotal NormTotalM041
Female-body		V109
Male-body
No Reads
dp5
GCCTAAAGCTGGAGAAATGGACCAAGCTGATAACCACCAACGAGTACAAGGTAAG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SizeHit CountTotal NormTotalM022
Male-body		M040
Female-body		M059
Embryo		M062
Head		V043
Embryo		V051
Head		V112
Male-body		SRR902010
Ovary		SRR902011
Testis		SRR902012
CNS imaginal disc
No Reads
droPer2
GACTAAAGCTGGAGAAATGGACCAAGCTGATAACCACCAACGAGTACAAGGTAAG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SizeHit CountTotal NormTotalM021
Embryo		M042
Female-body		V042
Embryo		V050
Head		V057
Head		V111
Male-body
No Reads
droAna3
CACA---------------------------------------------------------------------------------------------------------------------------------------------------------TTTACTGCCTGAATAATTTGCCACAGGTCCCA------------------GTGGTA-AACGAGTTGTCCTTTTT-TATAA-TTTA---------TSizeHit CountTotal NormTotalM044
Female-body		M058
Embryo		V039
Embryo		V055
Head		V105
Male-body		V106
Head
No Reads
droBip1
TCTGAAGCACTTTTACCTTGAT--------------------------------------------------------------------------------------------------------------------------TTC----GGCACATTTACTGTCTGAATAATTTGCCACAGCTCCCA------------------GTGGTA-AACGAGTTGTCCCTTTT-TTAAA-TTTA---------TSizeHit CountTotal NormTotal
No Reads
droKik1
GCCTTAAACTTGAAAAGTGGACGAAAATGATAACAACCAACGAGTTCAAGGTAAG---------------------------------------------------------------------------------------------------------------------------------------------------------------------TTGTATCATT-----------A---------TSizeHit CountTotal NormTotal
No Reads
droEle1
ATTGA------------------------------------------------------------------------------------------------------------------------------------------------------------CAGTTCGCATAATTGGCTGCTACTTTCG------------------CTGGTA-AACGAGTTGTCCCTTTT-TATAA-TTTATAATTT----SizeHit CountTotal NormTotal
No Reads
droBia1
CTTCTACT----------------------------------------------------------------------------------------------------------------------------------------TTC----CACGCATTTTCAGTTTGCATAATTGGCCTCTGCCTCCG------------------GCAGTA-AACGAGTTGTCCCTTTT--ATAA-TTTGTAATTT----SizeHit CountTotal NormTotal
No Reads
droTak1
CGAG---------------------------------------------------------------------------------------------------------------------------------------------------------ATTCCAGTTCGCATAATTGGCCGCTGCTTCCG------------------TTGGCAAAAAGAGTTGTCCTTTTT--ATAA-TTTATAATTT----SizeHit CountTotal NormTotal
No Reads
droEug1
TTTGA-------------------------------------------------------------------------------------------------------------------------------------------------------------TTTTAGCATAATTGGCTGTTTCTTCCT------------------TTGTTA-AAAGAGTTGTCCCTTTTTTATAA-TTTTTAATTT----SizeHit CountTotal NormTotal
No Reads
droSim2
TTTG-------------------------------------------------------------------------------------------------------------------------------------------------------------CAGTTCGCATAATTGGCTAATGCTTCCT-TTTCGAG----------ATATTC-AGCAGGTTGTCCCTTTT--ATAA-TGTATAATTTAAATSizeHit CountTotal NormTotalM023
Head		M024
Male-body		M025
Embryo		M053
Female-body		O001
Testis		O002
Head		SRR553485
Ovary		SRR553486
Ovary		SRR553487
Ovary		SRR553488
Ovary		SRR618934
Ovary		V044
Embryo		SRR902008
Ovary		SRR902009
Testis		SRR1275487
Male larvae		SRR1275485
Male prepupae		SRR1275483
Male prepupae
No Reads
droSec2
GTCTTAAGCTGGATAAGTGGACTAAAATGATAACAACCAACGAGTTTAGGGTGAG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SizeHit CountTotal NormTotalM054
Female-body		V113
Male-body		V114
Embryo		V115
Head
No Reads
droYak3
ATTTA------------------------------------------------------------------------------------------------------------------------------------------------------------CAGTTCGCATAATTGGCTGATGCTTCCT-TTTCGAG----------ATGTCG-AGCAGATTGTCCCTTTT--ATAA-TGTATAATTTAAATSizeHit CountTotal NormTotalM026
Head		M043
Female-body		M056
Embryo		V046
Embryo		V052
Head		V058
Head		V120
Male-body		SRR1275488
Male larvae		SRR1275486
Male prepupae		SRR1275484
Male prepupae		GSM1528802
follicle cells
No Reads
droEre2
TTTA-------------------------------------------------------------------------------------------------------------------------------------------------------------CAGTTTGCATAATTGGCTGATGCTCCCT-TTTCGAG----------ATGTTG-AGCAGGTTGTCCCTTTT--ATAA-TGTATAATTAAAATSizeHit CountTotal NormTotalM055
Female-body		M057
Embryo		V060
Head		V040
Embryo		V107
Male-body		V108
Head		GSM1528801
follicle cells
No Reads

Generated: 05/18/2015 at 04:33 AM