dwi_2464

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------################################################## AGACCAAAATACAAAGATCTCAACGAAAAGAGCTCAACAGAAAACACAAATAAAGACAGCAAAAGTGTCTTGCTCAGAAGAAGAGCGAAAGAGTATGAGCGGTTTCGGTTGTGCTCGCAACAAGACCGCAAGAAAAAAATCTGTCGACAGTTATTTGCGAGCTATGTTAGGGGGAAACAAAAGTAAAGCTCTTTTTTGTAGTCACTTATGATTGGTGATCTATTGCCCAATCCTCTGGCAAATCTTTCCGT ************************************************..((((((.......))))))((((.......))))((((((((....(((((((..(((((....))))).))))))).(((......))).(((.(((....))))))(((.((((.......))))..)))...))))))))......************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M045 female body	V118 embryo	V117 male body
...........................................................................................................................GACCGCAAGAAAAAAATCTGTCGACAGT....................................................................................................	28	0	2	0.50	1	1	0	0
....................CAACGAAAAGAGCTCAACAGAAAA...............................................................................................................................................................................................................	24	0	3	0.33	1	1	0	0
................................................................................................................GCTCGTCACACGACCGCAAG.......................................................................................................................	20	3	3	0.33	1	1	0	0
...............................................................................................TGAACGGCTTCGGTTGTCC.........................................................................................................................................	19	3	14	0.14	2	1	0	1
.................................................................................................................................................................CTTTGGTAGGGGGAAACAAGAGT...................................................................	23	3	7	0.14	1	0	1	0
................................................................................................................................................CGACAGTTATTTGCGAGCTATG.....................................................................................	22	0	10	0.10	1	1	0	0
................................................................................................GAACGGCTTCGGTTGTCC.........................................................................................................................................	18	3	20	0.05	1	1	0	0

TCTGGTTTTATGTTTCTAGAGTTGCTTTTCTCGAGTTGTCTTTTGTGTTTATTTCTGTCGTTTTCACAGAACGAGTCTTCTTCTCGCTTTCTCATACTCGCCAAAGCCAACACGAGCGTTGTTCTGGCGTTCTTTTTTTAGACAGCTGTCAATAAACGCTCGATACAATCCCCCTTTGTTTTCATTTCGAGAAAAAACATCAGTGAATACTAACCACTAGATAACGGGTTAGGAGACCGTTTAGAAAGGCA ************************************************..((((((.......))))))((((.......))))((((((((....(((((((..(((((....))))).))))))).(((......))).(((.(((....))))))(((.((((.......))))..)))...))))))))......************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M045 female body	V118 embryo
....................................TGTCTTTTGTGTTTATTTCTGTCGT..............................................................................................................................................................................................	25	0	1	1.00	1	1	0
............................................................................................................................TGGCGTTCTTTTTTTAGACAGCTGT......................................................................................................	25	0	2	0.50	1	0	1
........................................................................................................................................TTTAGACAGCTGTCAATAAACGCTCG.........................................................................................	26	0	2	0.50	1	1	0
......................................................................................................................................TTTTTAGACAGCTGTCAATAAACGC............................................................................................	25	0	2	0.50	1	1	0
.....................................................................................................................................TTTTTTAGACAGCTGTCAATAAACGC............................................................................................	26	0	2	0.50	1	0	1
..................GAGTTGCTTTTCTCGAGTTGTCTTTT...............................................................................................................................................................................................................	26	0	3	0.33	1	0	1
..............................................................................................................................................................................................................................AACGGCTTCGGAGACCGTTC.........	20	3	3	0.33	1	1	0
.......................................................................CGAGTCTTCGTCTCGCTAG.................................................................................................................................................................	19	3	17	0.06	1	1	0
..................................................................................................................................................TGTCAATAAACGCTCGATA......................................................................................	19	0	20	0.05	1	1	0

Species	Coordinate	ID	Alignment
droWil2	scf2_1100000004902:3390101-3390351 -	dwi_2464	AGACCAAAATACAAAGATCTCAACGAAAAGAGCTCAACAGAAAACACAAATAAAGACAGCAAAAGTGTCTTGCTCAGAAGAAGAGCGAAAGAGTA--TGAGCGGTTTCGGTTGTGCTCGCAACAAGACCGCAAGA--AAAAAATCTGTCGACAGTTATTTGCGAGCTATGTTAG---GGGGAAACAAAAGTAAAGCTCTTTTTTGTAGTCACTTATGATT----------GGTGATCTATTGCCCAATCCTCTGGCAAATCTTTCC--GT
droMoj3	scaffold_6680:16135217-16135429 +		AGAGCGACC-----A-CAGTCAACACTAAACGCTCAACCGAAAATCAAAACGAAAAGAACCGAAGGCTTTTGCTCAGAGCGGGAGCTGCAGAGCA--TAATT-ATTTCGGATGCTGCCTCAACCAGAACCAGGAA--AATTAATCGTATAGCAGTTATTTGCGAGCTATGTTAA---TCTTATACAATA--TAAT-CACTTATTCTTTATACTTATCATT----------AA----------------------------TAATCG--AT
droGri2	scaffold_15100:6649-6700 +		AGGCTCTGATGATAGG-------------------------------------------------------------------------------------------------------------------------------------------------TGATCTGCTGC-----------------------------------------------------------------------CGAATGCATTGTCAAATCTATCT--GT
dp5	Unknown_singleton_125:3777-3931 -		------------------CTCAACCGAAAACACAAAACACATAACACAAGCGAAGAGACAAAAAGTGTTCTGCTCAGTGAGAGA--------CCG--TGGTCTTTTTCTGTTTTGTCAGTGACGAGACAGCAAAG--GGAAAAAGTGTTGACCGTTGTTTGCGAGCTATGCCTG---GAACAAAGATA----------------------------------------------------------------------------------
droPer2	scaffold_43:260958-261112 +		------------------CTCAACCGAAAACACAAAACACATAACACAAGCGAAGAGACAAAAAGTGTTCTGCTCAGTGAGAGA--------CCG--TGGTCTTTTTCTGTTTTGTCAGTGACGAGACAGCAAAG--GGAAAAAGTGTTGACCGTTGTTTGCGAGCTATGCCTG---GAACAAAGATA----------------------------------------------------------------------------------
droAna3	scaffold_13247:68024-68213 -		CGACCGAAATAAAAAGAGCTCAACGA-AAACGCTCAACAGGAAACAGAAATAAAGATAGCAAAAGTCTCTTGCTCAGAGCGAGAGCGAAAAAGTA--TGAACTTTTTCGGTTGCGCTCGCAACGAGAGCACAAGA--AAAAAATCGGTCGACAGTTATTTGCAAGCTATGATAA---ATACCAAA---------A-----------------------------------------------------------CTC--TTTTTTT--TA
droBip1	scf7180000394164:2043-2236 +		CGACCGAAATAAAAAG-GCTCAACGAAAAGCGCTCAACAGAAAACAGAATTAAGGACAGCAAAAGTCTCTTGCTCAGAGCGAGAGCGAGAGAGTA--TGAACTTTTTCGGTTCTGCTCGCAACGAGAGCGCAAGA--AAAAAAACGGTTGACAGTTATTTACAAGCTCTGGAAA---AAACAA--------------------------------------------------------------------TAAAAATAATTGTCATAGT
droKik1	scf7180000302401:274569-274777 -		AGGCCGAAGCAAAAAGAGCTCAACGAAAAGCGTTCAACAAAAAACCGAAATGAAGACAGCAAAACTCCCTTGCTCTGAACGAGAGCGAGAGAGTA--TGATCTTTTTCGGTTTTGCTCGCACCGAGAGCACAGGA---AAAACCCCGTCGACAGTTATTTGCAAGCTCTGGTTTTGAGGCTAAATATAATTCTA-----------TGTATACTTTTAATT----------GT----------------------------T-------AA
droFic1	scf7180000453779:8795-8983 +		AGACCGAAATACAAAGAGCTCATCGAAAAGCGCTCAGCAGAGAACACAAATGATGACAGCAAGGGTCTCTTGCTCAGAGCGAGAGCGAGAGAGTA--TGAACTTTTTCGGTTGTGCTCGCATCGAGGGCACAAGA--TGAAAAACGGTCGACTGTTATTTGCGAGCTCTGTCTA---AAACACGTGTAAGCAAAG--------------------------------------------------------------------------C
droEle1	scf7180000487100:5915-6080 -		TTAGCATTACAAGAACAACTCAACTGAAAACAGTCAACCGAAAATCAAAACGAAGAGAAACGAAGTGTTCTGCTCAGATCGAAAGCAAC---GTACAA-GATTTTTTCGGTTGTGTTAGTGCATAGACCGTAAAG--CGAAAAACGGTTGACAGTTGTTTGCGAGCTATGCT--------------------------------------------------------------------------------------------------
droRho1	scf7180000776960:10296-10470 -		AGAGCGATATACAAAGATCTCAACGAAAAGCGCTCAACAGAAAACATAAATGAAGACAGC--AAGTCTCTTGCTCAGAGCGAGAGCGAGAGAGTA--TGAACTTTTTCGGTTGTGCTCGCAACGAGAGCGCAAGA--AGAAAAACGGTCAACAGTTGTTTGCGAGCTATGATAT---AAACAAA--------------------------------------------------------------------------------------
droBia1	scf7180000299577:7851-8051 -		CGACCGAAATAAAAAGGGCTCAACGAAAAGCGCTCATCAGAAAACAGAATTGAAGACAGCAAAAGTCTCTTGTTCAGAGCGAG------AGAGTA--TGAACTTTTTAGGTTGTGCTCGCAACGAGAGCACAAGAAAAAAAAAACGGTGGACAGTTATTTACAAGCTCTGATAA---TAAGCGAAATAA---T-----------------------------------------------------ATACAGATTCACATTTATAT--TC
droTak1	scf7180000410861:11468-11675 +		AGAGCGTCTCACGAACAGCTCAACGTAAAGGGCTCAACAGAAAACAAAAACGAAAGGAAACGAAGTCTGCTGCGCAGAGAGAGAACGAAGGGGCA--TACTATTTTCTGGTTGTCCTCGTGAGTAGAGCGTAAGG--AAAAAAACGGTCAACAGGTATGTGCATTCTTTGTTAA---AAACTTACATATCTAAAA------------CTTA-----------------------------------GCTTTTC------GTTTCTG--AT
droEug1	scf7180000409667:84082-84284 -		AGATC-----------AGCTCATAGTAATGAGCTGACAAGAAAACCAAAACAAAAAGAAACAATGTCTGCTGCTCTGAGAGAGAACGAAACTGCA--TGATTTTTTTCGGTTCTTCTGGCAAGCAGAGCGTGAG----GAAAAACGGTTAACAGTTATTTGCGAGCTATGGTTT---GAGGTAA-ATA---TTTC-CGCTACTTGTTTAAA----------------------------------------GCAACTATCTTTTAT--GT
dm3	chrYHet:532-589 +		T------------------------------------------------------------------------------------------------------TTTTAAGTCGCTTC-------------------------------------TTATTGGTGATCTATTAC-----------------------------------------------------------------------CGAACCCATTAGCAAATTTATCA--AT
droSec2	scaffold_3980:186-243 +		T------------------------------------------------------------------------------------------------------TTTTTAGTCGCTTC-------------------------------------TTATTGGTGATCTATTAC-----------------------------------------------------------------------CGAACCCATTAGCAAATTTATCA--GT
droYak3	2R:32778-33019 -		TGAGCATAACAAGAACCACGCGACTGAGAACTGTTGACCGAAAACCAAAACAAAGAGAAACGAAAAGTTCTGTTCAGAGCGAGAGCACACGTGCA--TCGTCTTTTTCGGTTGTGTTCGAGTGCAGACCGCAAAA--CGAAAAACGGTTGACAGTTATTTGCGAGCTATGCTCTGTAGGGGTCATGCAACTCAAA-GA---------TAGATCTTTAATGATTTAAGATTAGGGGTTAG------TAGTTTCTAGTAAATCT--------
droEre2	scaffold_4737:41415-41467 +		TAGTCGC------------------------------------------------------------------------------------------------------------------------------------------------TTCTTATTGGTGATCTATTAC-----------------------------------------------------------------------CAAATCCATTAGCAAATTTATCA--AT

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droWil2

AGACCAAAATACAAAGATCTCAACGAAAAGAGCTCAACAGAAAACACAAATAAAGACAGCAAAAGTGTCTTGCTCAGAAGAAGAGCGAAAGAGTA--TGAGCGGTTTCGGTTGTGCTCGCAACAAGACCGCAAGA--AAAAAATCTGTCGACAGTTATTTGCGAGCTATGTTAG---GGGGAAACAAAAGTAAAGCTCTTTTTTGTAGTCACTTATGATT----------GGTGATCTATTGCCCAATCCTCTGGCAAATCTTTCC--GT	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
.............................................................................................................................GACCGCAAGA--AAAAAATCTGTCGACAGT...................................................................................................................	28	2	0.50	1	0	1	0	0	0
....................CAACGAAAAGAGCTCAACAGAAAA..................................................................................................................................................................................................................................	24	3	0.33	1	0	1	0	0	0
....................................................................................................................................................CGACAGTTATTTGCGAGCTATG....................................................................................................	22	10	0.10	1	0	1	0	0	0

droMoj3

AGAGCGACC-----A-CAGTCAACACTAAACGCTCAACCGAAAATCAAAACGAAAAGAACCGAAGGCTTTTGCTCAGAGCGGGAGCTGCAGAGCA--TAATT-ATTTCGGATGCTGCCTCAACCAGAACCAGGAA--AATTAATCGTATAGCAGTTATTTGCGAGCTATGTTAA---TCTTATACAATA--TAAT-CACTTATTCTTTATACTTATCATT----------AA----------------------------TAATCG--AT	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
No Reads

droGri2

AGGCTCTGATGATAGG-------------------------------------------------------------------------------------------------------------------------------------------------TGATCTGCTGC-----------------------------------------------------------------------CGAATGCATTGTCAAATCTATCT--GT	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

dp5

------------------CTCAACCGAAAACACAAAACACATAACACAAGCGAAGAGACAAAAAGTGTTCTGCTCAGTGAGAGA--------CCG--TGGTCTTTTTCTGTTTTGTCAGTGACGAGACAGCAAAG--GGAAAAAGTGTTGACCGTTGTTTGCGAGCTATGCCTG---GAACAAAGATA----------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
............................................................................................................TGTTTTGTCAGTGACGAGACAGC...........................................................................................................................................	23	4	45.50	182	0	175	5	0	1	0	0	1	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--G....................................................................................................................................	24	11	42.55	468	0	420	4	0	7	2	0	32	3	0
.................................................................................................................................................TGTTGACCGTTGTTTGCGAGCTATGC...................................................................................................	26	4	40.25	161	0	158	2	0	1	0	0	0	0	0
.................................................................................................................................................TGTTGACCGTTGTTTGCGAGCTATG....................................................................................................	25	9	30.22	272	0	268	1	0	3	0	0	0	0	0
....................................................................................................................................................TGACCGTTGTTTGCGAGCTATGC...................................................................................................	23	4	28.50	114	0	103	8	0	3	0	0	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--.....................................................................................................................................	23	11	18.18	200	0	155	0	0	1	1	0	38	5	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--GG...................................................................................................................................	25	10	14.60	146	0	83	3	0	1	1	1	49	8	0
....................................................................................................................................................TGACCGTTGTTTGCGAGCTATG....................................................................................................	22	10	10.90	109	0	108	0	0	0	0	1	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--GGA..................................................................................................................................	26	10	10.70	107	0	22	0	0	0	0	0	79	6	0
.................................................................................................................................................TGTTGACCGTTGTTTGCGAGCT.......................................................................................................	22	10	10.20	102	0	100	1	0	1	0	0	0	0	0
.................................................................................................................................................TGTTGACCGTTGTTTGCGAGCTA......................................................................................................	23	9	10.11	91	0	89	0	0	1	0	1	0	0	0
............................................................................................................TGTTTTGTCAGTGACGAGACAGCA..........................................................................................................................................	24	4	6.50	26	0	25	0	0	0	0	0	1	0	0
.................................................................................................................................................TGTTGACCGTTGTTTGCGAGCTAT.....................................................................................................	24	9	5.78	52	0	50	1	0	1	0	0	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAA........................................................................................................................................	22	11	4.09	45	0	40	0	0	0	0	1	4	0	0
.................................................................................................................................................TGTTGACCGTTGTTTGCGAGC........................................................................................................	21	10	3.90	39	0	38	0	0	1	0	0	0	0	0
..................................................................................................................................................GTTGACCGTTGTTTGCGAGCTATGC...................................................................................................	25	4	3.75	15	0	15	0	0	0	0	0	0	0	0
.....................................................................................................................................................GACCGTTGTTTGCGAGCTATGC...................................................................................................	22	4	3.75	15	0	15	0	0	0	0	0	0	0	0
.................................................................................................................TGTCAGTGACGAGACAGCAAAG--G....................................................................................................................................	23	11	3.36	37	0	37	0	0	0	0	0	0	0	0
..................................................................................................................GTCAGTGACGAGACAGCAAAG--G....................................................................................................................................	22	14	3.00	42	0	35	1	0	1	0	0	5	0	0
....................................................................................................................................................TGACCGTTGTTTGCGAGCTATGCC..................................................................................................	24	1	3.00	3	0	1	2	0	0	0	0	0	0	0
..................................................CGAAGAGACAAAAAGTGTTCTGC.....................................................................................................................................................................................................	23	16	2.63	42	0	39	0	0	0	0	0	3	0	0
............................................................................................................TGTTTTGTCAGTGACGAGAC..............................................................................................................................................	20	5	2.60	13	0	12	0	0	1	0	0	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--GGAA.................................................................................................................................	27	10	2.60	26	0	12	1	0	0	0	0	13	0	0
.................................................................TGTTCTGCTCAGTGAGAGA--------CCG--TG...........................................................................................................................................................................	24	9	2.44	22	0	20	0	0	1	0	0	0	1	0
.............................................................................................................GTTTTGTCAGTGACGAGACAGC...........................................................................................................................................	22	15	2.40	36	0	36	0	0	0	0	0	0	0	0
...................................................................................................................TCAGTGACGAGACAGCAAAG--GG...................................................................................................................................	22	15	2.00	30	0	19	1	0	0	0	0	10	0	0
......................................................................................................................................................ACCGTTGTTTGCGAGCTATGC...................................................................................................	21	4	2.00	8	0	7	0	0	1	0	0	0	0	0
............................................................................................................TGTTTTGTCAGTGACGAGACAG............................................................................................................................................	22	5	2.00	10	0	9	0	0	1	0	0	0	0	0
..................................................................................................................................................GTTGACCGTTGTTTGCGAGCTATG....................................................................................................	24	10	2.00	20	0	19	0	0	0	0	1	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGC...........................................................................................................................................	19	15	1.93	29	0	28	0	0	1	0	0	0	0	0
.................................................................................................................TGTCAGTGACGAGACAGCAAAG--.....................................................................................................................................	22	11	1.64	18	0	18	0	0	0	0	0	0	0	0
............................................................................................................TGTTTTGTCAGTGACGAGACA.............................................................................................................................................	21	5	1.60	8	0	7	0	0	0	0	1	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--A....................................................................................................................................	24	11	1.55	17	0	15	0	0	0	0	0	2	0	0
.................................................................................................................................................TGTTGACCGTTGTTTGCGAG.........................................................................................................	20	10	1.50	15	0	14	0	0	1	0	0	0	0	0
..................................................................................................................GTCAGTGACGAGACAGCAAAG--GG...................................................................................................................................	23	13	1.46	19	0	17	2	0	0	0	0	0	0	0
....................................................................................................................................................TGACCGTTGTTTGCGAGCTAT.....................................................................................................	21	10	1.30	13	0	12	1	0	0	0	0	0	0	0
.................................................................................................................TGTCAGTGACGAGACAGCAAAG--GG...................................................................................................................................	24	10	1.20	12	0	12	0	0	0	0	0	0	0	0
...................................................................................................................TCAGTGACGAGACAGCAAAG--GGA..................................................................................................................................	23	14	1.14	16	0	13	0	0	1	0	1	1	0	0
................................................................................................................TTGTCAGTGACGAGACAGCA..........................................................................................................................................	20	15	1.07	16	0	15	1	0	0	0	0	0	0	0
..................................................CGAAGAGACAAAAAGTGTTCTGCT....................................................................................................................................................................................................	24	16	1.06	17	0	14	1	0	0	0	1	1	0	0
...................................................................................................................TCAGTGACGAGACAGCAAAG--GGAA.................................................................................................................................	24	14	1.00	14	0	9	0	0	0	0	1	4	0	0
....................................................................................................................................................TGACCGTTGTTTGCGAGCTATGCCT.................................................................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0
............................................................................................................TGTTTTGTCAGTGACGAGA...............................................................................................................................................	19	6	1.00	6	0	6	0	0	0	0	0	0	0	0
.................................................................................................................................................TGTTGACCGTTGTTTGCGAGCTATGCC..................................................................................................	27	1	1.00	1	0	0	1	0	0	0	0	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAA.........................................................................................................................................	21	15	0.93	14	0	14	0	0	0	0	0	0	0	0
.....................................................................................................................................................GACCGTTGTTTGCGAGCTATG....................................................................................................	21	11	0.91	10	0	10	0	0	0	0	0	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAA--.....................................................................................................................................	23	11	0.91	10	0	10	0	0	0	0	0	0	0	0
..................................................................................................................GTCAGTGACGAGACAGCAAAG--GGA..................................................................................................................................	24	12	0.83	10	0	9	1	0	0	0	0	0	0	0
...................................................................................................................TCAGTGACGAGACAGCAAAG--G....................................................................................................................................	21	16	0.81	13	0	11	2	0	0	0	0	0	0	0
....................................................................................................................................................TGACCGTTGTTTGCGAGCTA......................................................................................................	20	10	0.80	8	0	6	1	0	0	0	1	0	0	0
..................................................................................................................GTCAGTGACGAGACAGCAAAG--GGAA.................................................................................................................................	25	12	0.75	9	0	1	0	0	0	0	0	8	0	0
..................................................................................................................................................GTTGACCGTTGTTTGCGAGCT.......................................................................................................	21	11	0.73	8	0	8	0	0	0	0	0	0	0	0
.................................................................................................................................................TGTTGACCGTTGTTTGCGA..........................................................................................................	19	10	0.60	6	0	6	0	0	0	0	0	0	0	0
..................................................................GTTCTGCTCAGTGAGAGA--------CCG--TG...........................................................................................................................................................................	23	10	0.60	6	0	6	0	0	0	0	0	0	0	0
.................................................................................................................TGTCAGTGACGAGACAGCAAA........................................................................................................................................	21	11	0.55	6	0	6	0	0	0	0	0	0	0	0
.............................................................................................................GTTTTGTCAGTGACGAGACAGCA..........................................................................................................................................	23	15	0.53	8	0	8	0	0	0	0	0	0	0	0
............................................................................................................TGTTTTGTCAGTGACGAGACAGCAAAG--.....................................................................................................................................	27	4	0.50	2	0	2	0	0	0	0	0	0	0	0
.................................................................................................................TGTCAGTGACGAGACAGCAAAG--GGA..................................................................................................................................	25	10	0.50	5	0	4	0	0	0	0	0	1	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--GGAAA................................................................................................................................	28	10	0.50	5	0	4	0	0	0	0	0	0	1	0
..................................................CGAAGAGACAAAAAGTGTTCTG......................................................................................................................................................................................................	22	16	0.50	8	0	5	0	0	0	0	0	3	0	0
..................................................................................................................................................GTTGACCGTTGTTTGCGAGCTAT.....................................................................................................	23	10	0.50	5	0	5	0	0	0	0	0	0	0	0
..................................................CGAAGAGACAAAAAGTGTTCTGCTC...................................................................................................................................................................................................	25	16	0.50	8	0	7	0	0	0	0	0	0	1	0
............................................................................................................TGTTTTGTCAGTGACGAGACAGCAAAG--G....................................................................................................................................	28	4	0.50	2	0	2	0	0	0	0	0	0	0	0
..................................................................................................................................................GTTGACCGTTGTTTGCGAGCTA......................................................................................................	22	10	0.50	5	0	5	0	0	0	0	0	0	0	0
.................................................................................................................................................TGTTGACCGTTGTTTGCGAGCTATGT...................................................................................................	26	11	0.45	5	0	4	0	0	1	0	0	0	0	0
......................................................................................................................................................ACCGTTGTTTGCGAGCTATG....................................................................................................	20	11	0.45	5	0	5	0	0	0	0	0	0	0	0
.................................................................................................................................................TGTTGACCGTTGTTTGCGAGCTATA....................................................................................................	25	9	0.44	4	0	4	0	0	0	0	0	0	0	0
...............................................................................................................TTTGTCAGTGACGAGACAGCAAAG--GGA..................................................................................................................................	27	10	0.40	4	0	0	0	0	0	0	0	3	1	0
.............................................................................................................................................AAAGTGTTGACCGTTGTTTGCGAG.........................................................................................................	24	10	0.40	4	0	4	0	0	0	0	0	0	0	0
............................................................................................................TGTTTTGTCAGTGACGAGACAGA...........................................................................................................................................	23	5	0.40	2	0	2	0	0	0	0	0	0	0	0
..................................................................................................................GTCAGTGACGAGACAGCAAAG--.....................................................................................................................................	21	16	0.38	6	0	6	0	0	0	0	0	0	0	0
..................................................CGAAGAGACAAAAAGTGTTCT.......................................................................................................................................................................................................	21	16	0.38	6	0	5	0	0	1	0	0	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAA--A....................................................................................................................................	24	11	0.36	4	0	4	0	0	0	0	0	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--GGT..................................................................................................................................	26	11	0.36	4	0	1	0	0	0	0	0	3	0	0
.............................................................................................................GTTTTGTCAGTGACGAGAC..............................................................................................................................................	19	17	0.35	6	0	6	0	0	0	0	0	0	0	0
.........................................................................................................................................................GTTGTTTGCGAGCTATGC...................................................................................................	18	6	0.33	2	0	2	0	0	0	0	0	0	0	0
.....................................................................................................................AGTGACGAGACAGCAAAG--GGAAA................................................................................................................................	23	15	0.33	5	0	5	0	0	0	0	0	0	0	0
.................................................................................................................................................TGTTGACCGTTGTTTGCG...........................................................................................................	18	10	0.30	3	0	3	0	0	0	0	0	0	0	0
.............................................................................................................................................AAAGTGTTGACCGTTGTTTGCGAGC........................................................................................................	25	10	0.30	3	0	3	0	0	0	0	0	0	0	0
.................................................................................................................................................TGTTGACCGTTGTTTGCGAGA........................................................................................................	21	10	0.30	3	0	3	0	0	0	0	0	0	0	0
.................................................................TGTTCTGCTCAGTGAGAGA--------CCG--T............................................................................................................................................................................	23	11	0.27	3	0	3	0	0	0	0	0	0	0	0
...............................................................................................................TTTGTCAGTGACGAGACAGCAAAG--G....................................................................................................................................	25	11	0.27	3	0	3	0	0	0	0	0	0	0	0
..............................................................................................................TTTTGTCAGTGACGAGACAGC...........................................................................................................................................	21	15	0.27	4	0	4	0	0	0	0	0	0	0	0
.....................................................................................................................AGTGACGAGACAGCAAAG--GGAAAA...............................................................................................................................	24	15	0.27	4	0	1	0	0	1	0	2	0	0	0
....................................................................................................................CAGTGACGAGACAGCAAAG--GGA..................................................................................................................................	22	15	0.27	4	0	4	0	0	0	0	0	0	0	0
.....................................................................................................................AGTGACGAGACAGCAAAG--GGAA.................................................................................................................................	22	15	0.27	4	0	3	1	0	0	0	0	0	0	0
......................................................GAGACAAAAAGTGTTCTGCTCA..................................................................................................................................................................................................	22	16	0.25	4	0	3	0	0	0	0	0	1	0	0
............................................................................................................TGTTTTGTCAGTGACGAGACAGCAC.........................................................................................................................................	25	4	0.25	1	0	1	0	0	0	0	0	0	0	0
..................................................................................................................GTCAGTGACGAGACAGCAAAG--GGAAAA...............................................................................................................................	27	12	0.25	3	0	0	0	0	0	0	0	3	0	0
.......................................................................................................................................................CCGTTGTTTGCGAGCTATGC...................................................................................................	20	4	0.25	1	0	1	0	0	0	0	0	0	0	0
...................................................................................................................................................TTGACCGTTGTTTGCGAGCTATGC...................................................................................................	24	4	0.25	1	0	1	0	0	0	0	0	0	0	0
....................................................................................................................................AAG--GGAAAAAGTGTTGACCGTTGTT...............................................................................................................	25	8	0.25	2	0	2	0	0	0	0	0	0	0	0
...........................................................................................................CTGTTTTGTCAGTGACGAGACAGC...........................................................................................................................................	24	4	0.25	1	0	1	0	0	0	0	0	0	0	0
............................................................................................................TGTTTTGTCAGTGACGAGACAGCAAA........................................................................................................................................	26	4	0.25	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................................TGTTGACCGTTGTTTGCGAGCTATGCA..................................................................................................	27	4	0.25	1	0	1	0	0	0	0	0	0	0	0
.............................................................................................................GTTTTGTCAGTGACGAGACA.............................................................................................................................................	20	17	0.24	4	0	4	0	0	0	0	0	0	0	0
....................................................................................................................CAGTGACGAGACAGCAAAG--G....................................................................................................................................	20	17	0.24	4	0	4	0	0	0	0	0	0	0	0
...................................................................................................................TCAGTGACGAGACAGCAAAG--.....................................................................................................................................	20	18	0.22	4	0	4	0	0	0	0	0	0	0	0
................................................................................................................................................GTGTTGACCGTTGTTTGCGAGCTA......................................................................................................	24	9	0.22	2	0	1	0	0	0	0	1	0	0	0
.................................................................................................................................................TGTTGACCGTTGTTTGCGAGCTAA.....................................................................................................	24	9	0.22	2	0	2	0	0	0	0	0	0	0	0
.................................................................TGTTCTGCTCAGTGAGAGA--------CC................................................................................................................................................................................	21	15	0.20	3	0	3	0	0	0	0	0	0	0	0
..............................................................................................................................................AAGTGTTGACCGTTGTTTGCGAGC........................................................................................................	24	10	0.20	2	0	2	0	0	0	0	0	0	0	0
....................................................................................................................................................TGACCGTTGTTTGCGAGCTATGCG..................................................................................................	24	5	0.20	1	0	1	0	0	0	0	0	0	0	0
................................................................................................................................AGCAAAG--GGAAAAAGTGT..........................................................................................................................	18	15	0.20	3	0	3	0	0	0	0	0	0	0	0
...........................................................................................................CTGTTTTGTCAGTGACGAGAC..............................................................................................................................................	21	5	0.20	1	0	0	1	0	0	0	0	0	0	0
.......................................................................................................................TGACGAGACAGCAAAG--GGAAAAAGA............................................................................................................................	25	15	0.20	3	0	0	0	0	0	0	0	3	0	0
...............................................................................................................TTTGTCAGTGACGAGACAGCAAAG--GG...................................................................................................................................	26	10	0.20	2	0	0	0	0	0	0	0	1	1	0
......................................................GAGACAAAAAGTGTTCTGCTCAGT................................................................................................................................................................................................	24	16	0.19	3	0	2	1	0	0	0	0	0	0	0
.....................................................AGAGACAAAAAGTGTTCTGCTCA..................................................................................................................................................................................................	23	16	0.19	3	0	3	0	0	0	0	0	0	0	0
......................................................GAGACAAAAAGTGTTCTGCTCAG.................................................................................................................................................................................................	23	16	0.19	3	0	3	0	0	0	0	0	0	0	0
......................................................GAGACAAAAAGTGTTCTGCT....................................................................................................................................................................................................	20	16	0.19	3	0	3	0	0	0	0	0	0	0	0
....................................................................................................................CAGTGACGAGACAGCAAAG--GG...................................................................................................................................	21	16	0.19	3	0	3	0	0	0	0	0	0	0	0
.....................................................................................................................................................GACCGTTGTTTGCGAGCTA......................................................................................................	19	11	0.18	2	0	2	0	0	0	0	0	0	0	0
.....................................................................................................................................................GACCGTTGTTTGCGAGCTAT.....................................................................................................	20	11	0.18	2	0	2	0	0	0	0	0	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--GT...................................................................................................................................	25	11	0.18	2	0	1	0	0	0	0	0	1	0	0
......................................................................................................................................................ACCGTTGTTTGCGAGCTA......................................................................................................	18	11	0.18	2	0	0	0	0	0	0	0	2	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--GC...................................................................................................................................	25	11	0.18	2	0	2	0	0	0	0	0	0	0	0
....................................................................................................................................................TGACCGTTGTTTGCGAGCT.......................................................................................................	19	11	0.18	2	0	2	0	0	0	0	0	0	0	0
.....................................................................CTGCTCAGTGAGAGA--------CCG--TG...........................................................................................................................................................................	20	11	0.18	2	0	2	0	0	0	0	0	0	0	0
......................................................................TGCTCAGTGAGAGA--------CCG--TG...........................................................................................................................................................................	19	12	0.17	2	0	2	0	0	0	0	0	0	0	0
.................................................................................................................................................TGTTGACCGTTGTTTGCGAGCTATGCAA.................................................................................................	28	6	0.17	1	0	1	0	0	0	0	0	0	0	0
............................................................................................................TGTTTTGTCAGTGACGAGACAAA...........................................................................................................................................	23	6	0.17	1	0	1	0	0	0	0	0	0	0	0
............................................................................................................TGTTTTGTCAGTGACGAGAA..............................................................................................................................................	20	6	0.17	1	0	1	0	0	0	0	0	0	0	0
....................................ACACATAACACAAGCGAAGAGAC...................................................................................................................................................................................................................	23	13	0.15	2	0	1	0	0	0	0	0	1	0	0
.........................................TAACACAAGCGAAGAGACAAAA...............................................................................................................................................................................................................	22	13	0.15	2	0	0	0	0	0	0	0	2	0	0
....................................................................TCTGCTCAGTGAGAGA--------CCG--T............................................................................................................................................................................	20	13	0.15	2	0	2	0	0	0	0	0	0	0	0
..................................AAACACATAACACAAGCGAAGAGA....................................................................................................................................................................................................................	24	14	0.14	2	0	1	1	0	0	0	0	0	0	0
......................................................GAGACAAAAAGTGTTCTGCTCAGTG...............................................................................................................................................................................................	25	14	0.14	2	0	2	0	0	0	0	0	0	0	0
..................................AAACACATAACACAAGCGAAGAG.....................................................................................................................................................................................................................	23	14	0.14	2	0	2	0	0	0	0	0	0	0	0
...................................................................................................................TCAGTGACGAGACAGCAAAG--GGAAAA...............................................................................................................................	26	14	0.14	2	0	0	0	0	0	0	0	0	2	0
......................................ACATAACACAAGCGAAGAGACAAA................................................................................................................................................................................................................	24	14	0.14	2	0	0	0	0	0	0	0	2	0	0
......................................ACATAACACAAGCGAAGAGACA..................................................................................................................................................................................................................	22	14	0.14	2	0	1	0	0	0	0	0	1	0	0
.....................................................................................................................AGTGACGAGACAGCAAAG--GGAAAAAG.............................................................................................................................	26	14	0.14	2	0	1	0	0	0	0	0	1	0	0
.......................................CATAACACAAGCGAAGAGACA..................................................................................................................................................................................................................	21	14	0.14	2	0	0	0	0	0	0	0	2	0	0
.................................................................................................................TGTCAGTGACGAGACAGCAA.........................................................................................................................................	20	15	0.13	2	0	2	0	0	0	0	0	0	0	0
..............................................................................................................TTTTGTCAGTGACGAGACAGCA..........................................................................................................................................	22	15	0.13	2	0	2	0	0	0	0	0	0	0	0
.................................................................................................................TGTCAGTGACGAGACAGC...........................................................................................................................................	18	15	0.13	2	0	2	0	0	0	0	0	0	0	0
......................................................................................................................................G--GGAAAAAGTGTTGACCGTTGTTTGC............................................................................................................	26	8	0.13	1	0	1	0	0	0	0	0	0	0	0
.......................................................AGACAAAAAGTGTTCTGCTCAGT................................................................................................................................................................................................	23	16	0.13	2	0	2	0	0	0	0	0	0	0	0
..................................................................................................................................CAAAG--GGAAAAAGTGTTGACCGTTG.................................................................................................................	25	8	0.13	1	0	1	0	0	0	0	0	0	0	0
..................................................CGAAGAGACAAAAAGTGTTCTGCTCA..................................................................................................................................................................................................	26	16	0.13	2	0	1	0	0	0	0	0	1	0	0
.....................................................................................................................................AG--GGAAAAAGTGTTGACCGTTGT................................................................................................................	23	8	0.13	1	0	1	0	0	0	0	0	0	0	0
.....................................................................................................................................AG--GGAAAAAGTGTTGACCGTTG.................................................................................................................	22	8	0.13	1	0	1	0	0	0	0	0	0	0	0
...................................................GAAGAGACAAAAAGTGTTCTGC.....................................................................................................................................................................................................	22	16	0.13	2	0	2	0	0	0	0	0	0	0	0
.............................................................................................................GTTTTGTCAGTGACGAGACAGA...........................................................................................................................................	22	16	0.13	2	0	2	0	0	0	0	0	0	0	0
...................................................GAAGAGACAAAAAGTGTTCTGCT....................................................................................................................................................................................................	23	16	0.13	2	0	2	0	0	0	0	0	0	0	0
.....................................................................................................................................AG--GGAAAAAGTGTTGACCGTTGTT...............................................................................................................	24	8	0.13	1	0	0	1	0	0	0	0	0	0	0
.....................................................AGAGACAAAAAGTGTTCTGCT....................................................................................................................................................................................................	21	16	0.13	2	0	2	0	0	0	0	0	0	0	0
.....................................................................................................................AGTGACGAGACAGCAAAG--GG...................................................................................................................................	20	16	0.13	2	0	2	0	0	0	0	0	0	0	0
.............................................................AAAGTGTTCTGCTCAGTGAGAGA--------C.................................................................................................................................................................................	24	16	0.13	2	0	2	0	0	0	0	0	0	0	0
.............................................................................................................GTTTTGTCAGTGACGAGACAG............................................................................................................................................	21	16	0.13	2	0	2	0	0	0	0	0	0	0	0
...................................................GAAGAGACAAAAAGTGTTCTG......................................................................................................................................................................................................	21	16	0.13	2	0	2	0	0	0	0	0	0	0	0
.....................................................AGAGACAAAAAGTGTTCTGC.....................................................................................................................................................................................................	20	16	0.13	2	0	2	0	0	0	0	0	0	0	0
....................................................AAGAGACAAAAAGTGTTCTGCTCAG.................................................................................................................................................................................................	25	16	0.13	2	0	2	0	0	0	0	0	0	0	0
..................................................CGAAGAGACAAAAAGTGTT.........................................................................................................................................................................................................	19	16	0.13	2	0	1	1	0	0	0	0	0	0	0
......................................................GAGACAAAAAGTGTTCTGCTC...................................................................................................................................................................................................	21	16	0.13	2	0	2	0	0	0	0	0	0	0	0
....................................................AAGAGACAAAAAGTGTTCTGCTC...................................................................................................................................................................................................	23	16	0.13	2	0	2	0	0	0	0	0	0	0	0
...........................................................AAAAAGTGTTCTGCTCAGTG...............................................................................................................................................................................................	20	17	0.12	2	0	1	0	0	1	0	0	0	0	0
..........................................................................................................................CGAGACAGCAAAG--GGAAAAAG.............................................................................................................................	21	17	0.12	2	0	2	0	0	0	0	0	0	0	0
..................................................................................................................GTCAGTGACGAGACAGCAAA........................................................................................................................................	20	17	0.12	2	0	2	0	0	0	0	0	0	0	0
.......................................................AGACAAAAAGTGTTCTGCTCA..................................................................................................................................................................................................	21	18	0.11	2	0	2	0	0	0	0	0	0	0	0
...............................................................................................................................................AGTGTTGACCGTTGTTTGCGAGCTA......................................................................................................	25	9	0.11	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................................TGTTGACCGTTGTTTGCGAGCTAAA....................................................................................................	25	9	0.11	1	0	1	0	0	0	0	0	0	0	0
.............................................................................................................GTTTTGTCAGTGACGAGA...............................................................................................................................................	18	18	0.11	2	0	2	0	0	0	0	0	0	0	0
.................................................................TGTTCTGCTCAGTGAGAGA--------CCG--TGA..........................................................................................................................................................................	25	9	0.11	1	0	0	1	0	0	0	0	0	0	0
..............................................................................................................................................AAGTGTTGACCGTTGTTTGCGAGCTAT.....................................................................................................	27	9	0.11	1	0	1	0	0	0	0	0	0	0	0
........................................................GACAAAAAGTGTTCTGCTCAG.................................................................................................................................................................................................	21	19	0.11	2	0	2	0	0	0	0	0	0	0	0
................................................................GTGTTCTGCTCAGTGAGA............................................................................................................................................................................................	18	19	0.11	2	0	2	0	0	0	0	0	0	0	0
................................................................................................................................................GTGTTGACCGTTGTTTGCGA..........................................................................................................	20	10	0.10	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................................TGTTGACCGTTGTTTGCGAGCTC......................................................................................................	23	10	0.10	1	0	1	0	0	0	0	0	0	0	0
.............................................................................................................................................AAAGTGTTGACCGTTGTTTGCGAGCT.......................................................................................................	26	10	0.10	1	0	0	0	0	0	0	1	0	0	0
..............................................................................................................................................AAGTGTTGACCGTTGTTTGCGA..........................................................................................................	22	10	0.10	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................................TTTGTCAGTGACGAGACAGCAAAG--GGAA.................................................................................................................................	28	10	0.10	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................................................................AGTGTTGACCGTTGTTTGCGAGC........................................................................................................	23	10	0.10	1	0	1	0	0	0	0	0	0	0	0
..................................................................GTTCTGCTCAGTGAGAGA--------CCG--TGA..........................................................................................................................................................................	24	10	0.10	1	0	1	0	0	0	0	0	0	0	0
....................................................................................................................................................TGACCGTTGTTTGCGAGCTAG.....................................................................................................	21	10	0.10	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................................TGTTGACCGTTGTTTGCGAGCC.......................................................................................................	22	10	0.10	1	0	0	1	0	0	0	0	0	0	0
............................................................AAAAGTGTTCTGCTCAGTGA..............................................................................................................................................................................................	20	20	0.10	2	0	1	0	0	0	0	1	0	0	0
...............................................................................................................................................AGTGTTGACCGTTGTTTGCGA..........................................................................................................	21	10	0.10	1	0	0	0	0	0	0	1	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--GGAC.................................................................................................................................	27	10	0.10	1	0	0	0	0	0	0	0	1	0	0
.................................................................................................................TGTCAGTGACGAGACAGCAAAG--GGAA.................................................................................................................................	26	10	0.10	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................................................................AGTGTTGACCGTTGTTTGCGAGCT.......................................................................................................	24	10	0.10	1	0	1	0	0	0	0	0	0	0	0
...........................................................................................................................................AAAAAGTGTTGACCGTTGTTTGCGAGC........................................................................................................	27	10	0.10	1	0	1	0	0	0	0	0	0	0	0
......................................................................................................................................................ACCGTTGTTTGCGAGCTAT.....................................................................................................	19	11	0.09	1	0	1	0	0	0	0	0	0	0	0
.................................................................TGTTCTGCTCAGTGAGAGA--------CCG--.............................................................................................................................................................................	22	11	0.09	1	0	1	0	0	0	0	0	0	0	0
..................................................................................................................................................GTTGACCGTTGTTTGCGAGC........................................................................................................	20	11	0.09	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................................................CCGTTGTTTGCGAGCTATG....................................................................................................	19	11	0.09	1	0	1	0	0	0	0	0	0	0	0
.............................................................................................................GTTTTGTCAGTGACGAGACAGCAAA........................................................................................................................................	25	11	0.09	1	0	1	0	0	0	0	0	0	0	0
...................................................................TTCTGCTCAGTGAGAGA--------CCG--TG...........................................................................................................................................................................	22	11	0.09	1	0	1	0	0	0	0	0	0	0	0
...................................................................................................................................................TTGACCGTTGTTTGCGAGCT.......................................................................................................	20	11	0.09	1	0	1	0	0	0	0	0	0	0	0
......................................................................................................................................G--GGAAAAAGTGTTGACCGTT..................................................................................................................	20	11	0.09	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................................TGTTGACCGTTGTTTGCGAA.........................................................................................................	20	11	0.09	1	0	1	0	0	0	0	0	0	0	0
....................................................................................................................................................TGACCGTTGTTTGCGAGC........................................................................................................	18	11	0.09	1	0	1	0	0	0	0	0	0	0	0
.............................................................................................................................................AAAGTGTTGACCGTTGTTTGC............................................................................................................	21	11	0.09	1	0	1	0	0	0	0	0	0	0	0
..............................................................AAGTGTTCTGCTCAGTGAGAGA--------CCG--.............................................................................................................................................................................	25	11	0.09	1	0	1	0	0	0	0	0	0	0	0
..................................................................................................................................................GTTGACCGTTGTTTGCGA..........................................................................................................	18	11	0.09	1	0	1	0	0	0	0	0	0	0	0
.............................................................................................................................................AAAGTGTTGACCGTTGTTTG.............................................................................................................	20	11	0.09	1	0	1	0	0	0	0	0	0	0	0
....................................................................................................................................................TGACCGTTGTTTGCGAGCTG......................................................................................................	20	11	0.09	1	0	1	0	0	0	0	0	0	0	0
....................................................................TCTGCTCAGTGAGAGA--------CCG--TG...........................................................................................................................................................................	21	11	0.09	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................TGTCAGTGACGAGACAGCAAAA--A....................................................................................................................................	23	11	0.09	1	0	1	0	0	0	0	0	0	0	0
..............................................................................................................................................AAGTGTTGACCGTTGTTTGC............................................................................................................	20	11	0.09	1	0	1	0	0	0	0	0	0	0	0
.......................................................AGACAAAAAGTGTTCTGCTCAGTGAGA............................................................................................................................................................................................	27	12	0.08	1	0	1	0	0	0	0	0	0	0	0
..................................................................GTTCTGCTCAGTGAGAGA--------CCG--.............................................................................................................................................................................	21	12	0.08	1	0	1	0	0	0	0	0	0	0	0
..........................................................................................................................CGAGACAGCAAAG--GGAAAAAGTGT..........................................................................................................................	24	12	0.08	1	0	1	0	0	0	0	0	0	0	0
.....................................................................................................................................................GACCGTTGTTTGCGAGCTATT....................................................................................................	21	12	0.08	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................................................CCGTTGTTTGCGAGCTATT....................................................................................................	19	12	0.08	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................TGACGAGACAGCAAAG--GGAAAAAGTG...........................................................................................................................	26	12	0.08	1	0	1	0	0	0	0	0	0	0	0
...........................................................................................................................GAGACAGCAAAG--GGAAAAAGTGT..........................................................................................................................	23	12	0.08	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................TGACGAGACAGCAAAG--GGAAAAAGT............................................................................................................................	25	12	0.08	1	0	1	0	0	0	0	0	0	0	0
..................................AAACACATAACACAAGCGAAGAGAC...................................................................................................................................................................................................................	25	12	0.08	1	0	0	1	0	0	0	0	0	0	0
............................................................AAAAGTGTTCTGCTCAGTGAGAGA--------CC................................................................................................................................................................................	26	13	0.08	1	0	1	0	0	0	0	0	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--T....................................................................................................................................	24	13	0.08	1	0	1	0	0	0	0	0	0	0	0
.......................................CATAACACAAGCGAAGAGACAAAA...............................................................................................................................................................................................................	24	13	0.08	1	0	0	0	0	0	0	0	0	1	0
.........................................................ACAAAAAGTGTTCTGCTCAGTGAGAGA--------C.................................................................................................................................................................................	28	13	0.08	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................................................CAGCAAAG--GGAAAAAGTGTTGACCGC...................................................................................................................	26	13	0.08	1	0	0	1	0	0	0	0	0	0	0
........................................................................................................................................................CGTTGTTTGCGAGCTATG....................................................................................................	18	13	0.08	1	0	1	0	0	0	0	0	0	0	0
...........................................................................................................................GAGACAGCAAAG--GGAAAAAGTG...........................................................................................................................	22	13	0.08	1	0	1	0	0	0	0	0	0	0	0
................................................................................................................................AGCAAAG--GGAAAAAGTGTTGACCG....................................................................................................................	24	13	0.08	1	0	1	0	0	0	0	0	0	0	0
....................................ACACATAACACAAGCGAAGAGACA..................................................................................................................................................................................................................	24	13	0.08	1	0	1	0	0	0	0	0	0	0	0

droPer2

------------------CTCAACCGAAAACACAAAACACATAACACAAGCGAAGAGACAAAAAGTGTTCTGCTCAGTGAGAGA--------CCG--TGGTCTTTTTCTGTTTTGTCAGTGACGAGACAGCAAAG--GGAAAAAGTGTTGACCGTTGTTTGCGAGCTATGCCTG---GAACAAAGATA----------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--G....................................................................................................................................	24	16	12.00	192	0	182	9	0	0	1
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--.....................................................................................................................................	23	16	4.81	77	0	76	1	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--GG...................................................................................................................................	25	14	4.71	66	0	64	2	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAA........................................................................................................................................	22	16	1.88	30	0	30	0	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--GGA..................................................................................................................................	26	14	1.64	23	0	21	1	0	0	1
.................................................................................................................TGTCAGTGACGAGACAGCAAAG--G....................................................................................................................................	23	16	1.38	22	0	22	0	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAA.........................................................................................................................................	21	19	0.84	16	0	16	0	0	0	0
.................................................................................................................TGTCAGTGACGAGACAGCAAAG--.....................................................................................................................................	22	16	0.56	9	0	9	0	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGC...........................................................................................................................................	19	19	0.47	9	5	3	1	0	0	0
..................................................................................................................................CAAAG--GGAAAAAGTGTTG........................................................................................................................	18	20	0.40	8	0	6	0	0	0	2
................................................................................................................TTGTCAGTGACGAGACAGCA..........................................................................................................................................	20	19	0.37	7	0	7	0	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAA--.....................................................................................................................................	23	16	0.31	5	0	5	0	0	0	0
.................................................................................................................TGTCAGTGACGAGACAGCAAA........................................................................................................................................	21	16	0.31	5	0	5	0	0	0	0
.................................................................TGTTCTGCTCAGTGAGAGA--------CCG--TG...........................................................................................................................................................................	24	20	0.30	6	0	6	0	0	0	0
.................................................................................................................TGTCAGTGACGAGACAGCAAAG--GG...................................................................................................................................	24	14	0.29	4	0	4	0	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--GGAA.................................................................................................................................	27	14	0.29	4	0	4	0	0	0	0
.................................................................................................................TGTCAGTGACGAGACAGCAAAG--GGA..................................................................................................................................	25	14	0.21	3	0	3	0	0	0	0
...............................................................................................................TTTGTCAGTGACGAGACAGCAAAG--GGAA.................................................................................................................................	28	14	0.21	3	3	0	0	0	0	0
..................................................................................................................GTCAGTGACGAGACAGCAAAG--G....................................................................................................................................	22	19	0.21	4	0	4	0	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--A....................................................................................................................................	24	17	0.18	3	0	3	0	0	0	0
............................................................................................................TGTTTTGTCAGTGACGAGACAGC...........................................................................................................................................	23	6	0.17	1	0	0	1	0	0	0
...............................................................................................................................................AGTGTTGACCGTTGTTTGCGAGCT.......................................................................................................	24	16	0.13	2	0	1	0	0	0	1
................................................................................................................TTGTCAGTGACGAGACAG............................................................................................................................................	18	19	0.11	2	0	2	0	0	0	0
...................................................................................................................................................TTGACCGTTGTTTGCGAGCTATG....................................................................................................	23	14	0.07	1	0	1	0	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--GGT..................................................................................................................................	26	14	0.07	1	0	1	0	0	0	0
......................................................................................................................................................ACCGTTGTTTGCGAGCTATG....................................................................................................	20	15	0.07	1	0	1	0	0	0	0
..................................................................................................................................................GTTGACCGTTGTTTGCGAGC........................................................................................................	20	16	0.06	1	0	1	0	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--C....................................................................................................................................	24	16	0.06	1	0	1	0	0	0	0
.................................................................................................................TGTCAGTGACGAGACAGCAAAA--A....................................................................................................................................	23	17	0.06	1	0	1	0	0	0	0
............................................................................................................................................AAAAGTGTTGACCGTTGTTTG.............................................................................................................	21	17	0.06	1	0	1	0	0	0	0
..................................................................................................................GTCAGTGACGAGACAGCAAAG--GGAA.................................................................................................................................	25	17	0.06	1	1	0	0	0	0	0
..................................................................................................................GTCAGTGACGAGACAGCAAAG--GG...................................................................................................................................	23	17	0.06	1	0	1	0	0	0	0
............................................................................................................................................AAAAGTGTTGACCGTTGTTTGC............................................................................................................	22	17	0.06	1	0	1	0	0	0	0
..................................AAACACATAACACAAGCGAAGAG.....................................................................................................................................................................................................................	23	17	0.06	1	0	1	0	0	0	0
................................................................................................................TTGTCAGTGACGAGACAGCAAAG--T....................................................................................................................................	24	18	0.06	1	0	0	1	0	0	0
.......................................................................................................................TGACGAGACAGCAAAG--GGAAAAAGT............................................................................................................................	25	18	0.06	1	0	0	0	0	0	1
.................................................................................................................TGTCAGTGACGAGACAGCAA.........................................................................................................................................	20	19	0.05	1	0	1	0	0	0	0
......................................................GAGACAAAAAGTGTTCTGCTCAGTGA..............................................................................................................................................................................................	26	19	0.05	1	0	1	0	0	0	0
.................................................................TGTTCTGCTCAGTGAGAGA--------CCG--.............................................................................................................................................................................	22	20	0.05	1	0	1	0	0	0	0
.......................................................................................................................TGACGAGACAGCAAAG--GGAAAAAG.............................................................................................................................	24	20	0.05	1	0	0	0	0	0	1
...................................................................................................................TCAGTGACGAGACAGCAAAG--G....................................................................................................................................	21	20	0.05	1	0	1	0	0	0	0
..............................................................AAGTGTTCTGCTCAGTGAGAGA--------CCG--.............................................................................................................................................................................	25	20	0.05	1	0	0	1	0	0	0
.................................................................TGTTCTGCTCAGTGAGAGA--------C.................................................................................................................................................................................	20	20	0.05	1	1	0	0	0	0	0
......................................................................TGCTCAGTGAGAGA--------CCG--TG...........................................................................................................................................................................	19	20	0.05	1	0	1	0	0	0	0
....................................................................................................................CAGTGACGAGACAGCAAAG--GGAAAAA..............................................................................................................................	26	20	0.05	1	0	0	1	0	0	0
.................................................................TGTTCTGCTCAGTGAGAGA--------CCG--T............................................................................................................................................................................	23	20	0.05	1	0	1	0	0	0	0
......................................................GAGACAAAAAGTGTTCTGCT....................................................................................................................................................................................................	20	20	0.05	1	0	1	0	0	0	0
..............................................................AAGTGTTCTGCTCAGTGAGAGA--------..................................................................................................................................................................................	22	20	0.05	1	0	0	0	0	0	1
..................................................................................................................GTCAGTGACGAGACAGCAAA........................................................................................................................................	20	20	0.05	1	0	1	0	0	0	0
...................................................................TTCTGCTCAGTGAGAGA--------CCG--.............................................................................................................................................................................	20	20	0.05	1	0	1	0	0	0	0
...................................................................TTCTGCTCAGTGAGAGA--------CCG--TG...........................................................................................................................................................................	22	20	0.05	1	0	1	0	0	0	0
..................................................................GTTCTGCTCAGTGAGAGA--------CCG--TG...........................................................................................................................................................................	23	20	0.05	1	0	1	0	0	0	0
................................................................................................................TTGTCAGTGACGAGACAAA...........................................................................................................................................	19	20	0.05	1	0	1	0	0	0	0
.................................................................TGTTCTGCTCAGTGAGAGA--------CCG--TGA..........................................................................................................................................................................	25	20	0.05	1	0	1	0	0	0	0
..................................................................................................................GTCAGTGACGAGACAGCAAAG--.....................................................................................................................................	21	20	0.05	1	0	1	0	0	0	0
......................................................................................................................................G--GGAAAAAGTGTTGACCGT...................................................................................................................	19	20	0.05	1	0	1	0	0	0	0

droAna3

CGACCGAAATAAAAAGAGCTCAACGA-AAACGCTCAACAGGAAACAGAAATAAAGATAGCAAAAGTCTCTTGCTCAGAGCGAGAGCGAAAAAGTA--TGAACTTTTTCGGTTGCGCTCGCAACGAGAGCACAAGA--AAAAAATCGGTCGACAGTTATTTGCAAGCTATGATAA---ATACCAAA---------A-----------------------------------------------------------CTC--TTTTTTT--TA	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
.GACCGAAATAAAAAGAGCTCAACGA-...................................................................................................................................................................................................................................................	25	15	0.13	2	2	0	0	0	0	0
.GACCGAAATAAAAAGAGCTCAACGA-A..................................................................................................................................................................................................................................................	26	15	0.13	2	1	1	0	0	0	0
...............................................................AGTCTCTTGCTCAGAGCGAGC..........................................................................................................................................................................................	21	10	0.10	1	0	0	0	1	0	0
...................TCAACGA-AAACGCTCAACAGGAAACAGA..............................................................................................................................................................................................................................	28	14	0.07	1	1	0	0	0	0	0
.....GAAATAAAAAGAGCTCAACGA-AAACGCT............................................................................................................................................................................................................................................	28	14	0.07	1	0	1	0	0	0	0
....................CAACGA-AAACGCTCAACAGGAAACAGA..............................................................................................................................................................................................................................	27	14	0.07	1	1	0	0	0	0	0
..................CTCAACGA-AAACGCTCAACAGGAAACAG...............................................................................................................................................................................................................................	28	14	0.07	1	1	0	0	0	0	0
..................CTCAACGA-AAACGCTCAACAGGAAACA................................................................................................................................................................................................................................	27	14	0.07	1	1	0	0	0	0	0
...................TCAACGA-AAACGCTCAACAGGAAACAG...............................................................................................................................................................................................................................	27	14	0.07	1	0	1	0	0	0	0
...................................................................................................................................................TCGACAGTTATTTGCAAGC........................................................................................................	19	15	0.07	1	0	1	0	0	0	0
.....................AACGA-AAACGCTCAACAGGAAACAG...............................................................................................................................................................................................................................	25	15	0.07	1	1	0	0	0	0	0
..ACCGAAATAAAAAGAGCTCAACGA-AA.................................................................................................................................................................................................................................................	26	15	0.07	1	1	0	0	0	0	0
..ACCGAAATAAAAAGAGCTCAACGA-A..................................................................................................................................................................................................................................................	25	15	0.07	1	1	0	0	0	0	0

droBip1

CGACCGAAATAAAAAG-GCTCAACGAAAAGCGCTCAACAGAAAACAGAATTAAGGACAGCAAAAGTCTCTTGCTCAGAGCGAGAGCGAGAGAGTA--TGAACTTTTTCGGTTCTGCTCGCAACGAGAGCGCAAGA--AAAAAAACGGTTGACAGTTATTTACAAGCTCTGGAAA---AAACAA--------------------------------------------------------------------TAAAAATAATTGTCATAGT	Size	Hit Count	Total Norm	Total
No Reads

droKik1

AGGCCGAAGCAAAAAGAGCTCAACGAAAAGCGTTCAACAAAAAACCGAAATGAAGACAGCAAAACTCCCTTGCTCTGAACGAGAGCGAGAGAGTA--TGATCTTTTTCGGTTTTGCTCGCACCGAGAGCACAGGA---AAAACCCCGTCGACAGTTATTTGCAAGCTCTGGTTTTGAGGCTAAATATAATTCTA-----------TGTATACTTTTAATT----------GT----------------------------T-------AA	Size	Hit Count	Total Norm	Total
No Reads

droFic1

AGACCGAAATACAAAGAGCTCATCGAAAAGCGCTCAGCAGAGAACACAAATGATGACAGCAAGGGTCTCTTGCTCAGAGCGAGAGCGAGAGAGTA--TGAACTTTTTCGGTTGTGCTCGCATCGAGGGCACAAGA--TGAAAAACGGTCGACTGTTATTTGCGAGCTCTGTCTA---AAACACGTGTAAGCAAAG--------------------------------------------------------------------------C	Size	Hit Count	Total Norm	Total
No Reads

droEle1

TTAGCATTACAAGAACAACTCAACTGAAAACAGTCAACCGAAAATCAAAACGAAGAGAAACGAAGTGTTCTGCTCAGATCGAAAGCAAC---GTACAA-GATTTTTTCGGTTGTGTTAGTGCATAGACCGTAAAG--CGAAAAACGGTTGACAGTTGTTTGCGAGCTATGCT--------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droRho1

AGAGCGATATACAAAGATCTCAACGAAAAGCGCTCAACAGAAAACATAAATGAAGACAGC--AAGTCTCTTGCTCAGAGCGAGAGCGAGAGAGTA--TGAACTTTTTCGGTTGTGCTCGCAACGAGAGCGCAAGA--AGAAAAACGGTCAACAGTTGTTTGCGAGCTATGATAT---AAACAAA--------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droBia1

CGACCGAAATAAAAAGGGCTCAACGAAAAGCGCTCATCAGAAAACAGAATTGAAGACAGCAAAAGTCTCTTGTTCAGAGCGAG------AGAGTA--TGAACTTTTTAGGTTGTGCTCGCAACGAGAGCACAAGAAAAAAAAAACGGTGGACAGTTATTTACAAGCTCTGATAA---TAAGCGAAATAA---T-----------------------------------------------------ATACAGATTCACATTTATAT--TC	Size	Hit Count	Total Norm	Total
No Reads

droTak1

AGAGCGTCTCACGAACAGCTCAACGTAAAGGGCTCAACAGAAAACAAAAACGAAAGGAAACGAAGTCTGCTGCGCAGAGAGAGAACGAAGGGGCA--TACTATTTTCTGGTTGTCCTCGTGAGTAGAGCGTAAGG--AAAAAAACGGTCAACAGGTATGTGCATTCTTTGTTAA---AAACTTACATATCTAAAA------------CTTA-----------------------------------GCTTTTC------GTTTCTG--AT	Size	Hit Count	Total Norm	Total
No Reads

droEug1

AGATC-----------AGCTCATAGTAATGAGCTGACAAGAAAACCAAAACAAAAAGAAACAATGTCTGCTGCTCTGAGAGAGAACGAAACTGCA--TGATTTTTTTCGGTTCTTCTGGCAAGCAGAGCGTGAG----GAAAAACGGTTAACAGTTATTTGCGAGCTATGGTTT---GAGGTAA-ATA---TTTC-CGCTACTTGTTTAAA----------------------------------------GCAACTATCTTTTAT--GT	Size	Hit Count	Total Norm	Total
No Reads

dm3

T------------------------------------------------------------------------------------------------------TTTTAAGTCGCTTC-------------------------------------TTATTGGTGATCTATTAC-----------------------------------------------------------------------CGAACCCATTAGCAAATTTATCA--AT

Size

Hit Count

Total Norm

Total

GSM154618
Ovary

GSM154620
Ovary

GSM154621
Ovary

GSM154622
Ovary

GSM180328
Head

GSM180329
Mixed-body

GSM180330
Embryo

GSM180331
Embryo

GSM180332
Embryo

GSM180333
Embryo

GSM180334
Larvae

GSM180335
Disc

GSM180336
Pupae

GSM180337
S2

GSM231091
Ovary

GSM239041
Head

GSM239050
Head

GSM239051
S2

GSM239052
S2

GSM246084
Head

GSM266765
S2

GSM280082
Ovary

GSM280083
Ovary

GSM280084
Ovary

GSM280085
Testis

GSM280086
Ovary

GSM280087
S2-NP

GSM280088
S2-NP

GSM312995
Head

GSM313160
Head

GSM313161
Head

GSM313162
Head

GSM313163
Head

GSM313164
Head

GSM313165
Head

GSM343287
Embryo

GSM379050
Ovary

GSM379051
Ovary

GSM379052
Ovary

GSM379053
Ovary

GSM379054
Ovary

GSM379055
Ovary

GSM379056
Ovary

GSM379057
Ovary

GSM379058
Ovary

GSM379059
Ovary

GSM379060
Ovary

GSM379061
Ovary

GSM379062
Ovary

GSM379063
Ovary

GSM379064
Ovary

GSM379065
Ovary

GSM379066
Ovary

GSM379067
Ovary

GSM424739
S2

GSM424740
S2

GSM424741
S2

GSM467729
Female-body

GSM467730
Female-body

GSM467731
Female-body

M002
CS

M003
Male-body

M004
Male-body

M005
ML-DmD32

M006
ML-DmD32

M029
OSC

M030
OSC

M031
OSC

M032
S2-R+

M033
S2-R+

M034
S2-R+

M035
S2-R+

M036
S2-R+

M037
S2-R+

M038
S2-R+

M039
S2-R+

M048
S2-R+

OSS2
OSS

OSS6
OSS

OSS7
OSS

OSS8
OSS

S1
Head

S10
Embryo

S11
Embryo

S12
Embryo

S13
Embryo

S14
Embryo

S15
S2-GMR

S16
Kc

S17
Larvae

S18
Larvae

S19
Pupae

S2
Head

S20
Pupae

S21
Disc

S22
Head

S23
Female-body

S24
Male-body

S25
Larvae

S26
Larvae

S27
Pupae

S28
Pupae

S29
Head

S3
Male-body

S30
S2-GMR

S31
S2

S32
S2

S33
Kc

S34
Kc

S35
Kc

S4
Female-body

S5
Disc

S6
Embryo

S7
Embryo

S8
S2

S9
Embryo

SRR001337
Head

SRR001338
Head

SRR001339
Head

SRR001340
Head

SRR001341
Head

SRR001342
Head

SRR001343
Head

SRR001344
Head

SRR001345
Head

SRR001346
Head

SRR001347
Head

SRR001348
Head

SRR001349
Head

SRR001664
Head

SRR010951
Ovary

SRR010952
Ovary

SRR010953
Ovary

SRR010954
Ovary

SRR010955
Ovary

SRR010956
Ovary

SRR010957
Ovary

SRR010958
Ovary

SRR010959
Ovary

SRR010960
Ovary

SRR014268
Embryo

SRR014269
Embryo

SRR014270
Embryo

SRR014271
Embryo

SRR014272
Embryo

SRR014273
Ovary

SRR014274
Ovary

SRR014275
Ovary

SRR014276
Ovary

SRR014277
Ovary

SRR014278
Ovary

SRR014279
Ovary

SRR014280
Ovary

SRR014281
Ovary

SRR014282
Ovary

SRR023197
S2

SRR023399
S2

SRR023400
S2

SRR023402
S2

SRR023407
S2

SRR029028
S2

SRR029029
S2

SRR029030
S2

SRR029031
S2

SRR029032
S2

SRR029033
S2

SRR029608
Head

SRR029633
Head

SRR030145
gb3

SRR031692
Head

SRR031693
Head

SRR031694
Head

SRR031696
Head

SRR031697
Head

SRR031698
Head

SRR031699
Head

SRR031700
Head

SRR031701
Head

SRR031702
Head

SRR031703
Head

SRR031704
Head

SRR032092
S2

SRR032093
S2

SRR032094
S2

SRR032095
S2-NP

SRR032096
S2-NP

SRR032152
Head

SRR060643
Testis

SRR060644
Ovary

SRR060645
Testis

SRR060646
Ovary

SRR060647
Ovary

SRR060648
Ovary

SRR060649
Ovary

SRR060650
Testis

SRR060651
Ovary

SRR060652
Testis

SRR060653
Ovary

SRR065151
Ovary

SRR065152
Ovary

SRR065153
Ovary

SRR065154
Ovary

SRR065155
Ovary

SRR065800
Ovary

SRR065801
Ovary

SRR065802
Ovary

SRR065803
Ovary

SRR065804
Ovary

SRR065805
Ovary

SRR065806
Ovary

SRR065807
Ovary

SRR097865
S2-NP

SRR097866
S2-NP

SRR097867
S2-NP

SRR298536
Ovary

SRR298537
Ovary

SRR298711
Ovary

SRR298712
Ovary

SRR317110
Ovary

SRR317111
Ovary

SRR317112
Ovary

SRR317113
Ovary

SRR317114
Ovary

SRR317115
Ovary

SRR317116
Ovary

SRR317117
Ovary

SRR341115
S2

SRR341116
S2

SRR341117
S2

SRR341118
S2

SRR351332
Mixed-body

SRR351333
Mixed-body

T004
OSC

T005
CME-L1

V001
Embryo

V002
Embryo

V003
S2

V006
Female-body

V007
Head

V008
S2

V009
CME-L1

V010
ML-DmD20-C5

V011
Sg4

V012
Male-body

V013
Head

V014
DTT

V015
DreRFHV

V018
Kc167

V019
GM2

V020
S2-R+

V021
ML-DmD21

V022
ML-DmD32

V023
Head

V024
Kc167

V025
S2-R+

V026
1182-2H

V027
ML-DmD21

V028
CME-W1-Cl.8+

V029
ML-DmD9

V030
ML-DmD8

V031
GM2

V032
S1

V033
CME-W1-Cl.8+

V034
ML-DmD16-C3

V035
ML-DmD32

V036
ML-DmD20-C5

V037
S2

V038
S2

V063
Ovary

V0632
Ovary

V064
Ovary

V0642
Ovary

V065
Ovary

V0652
Ovary

V066
Ovary

V0662
Ovary

V067
S2-NP

V073
mbn2

V074
S3

V075
ML-DmBG1-C1

V076
ML-DmBG3-C2

V077
Head

V078
Head

V079
Head

V080
Head

V081
Embryo

V082
Embryo

V083
Male-body

V084
Female-body

V085
CME-W2

V086
Female-body

V088
Ovary

V0882
Ovary

V089
Ovary

V0892
Ovary

V090
Male-body

V091
fGS/OSS

V092
Male-body

V093
Male-body

V094
S2-R+

V095
S2-R+

V096
Ovary

V097
Ovary

V098
Ovary

V125
ML-DmD9

V126
CME-L1

V127
G2

V128
S3

V129
ML-DmBG1-C1

V130
ML-DmBG3-C2

V131
ML-DmD16-C3

V132
ML-DmD32

V133
Sg4

V134
ML-DmD8

V135
CME-W2

V136
Male-body

V137
Head

V138
Male-body

V139
Female-body

V140
Female-body

V141
Female-body

V142
Female-body

V144
OSC

V145
S2

V146
S1

V147
1182-2H

V148
mbn2

GSM1528798
follicle cells

No Reads

droSec2

T------------------------------------------------------------------------------------------------------TTTTTAGTCGCTTC-------------------------------------TTATTGGTGATCTATTAC-----------------------------------------------------------------------CGAACCCATTAGCAAATTTATCA--GT	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
No Reads

droYak3

TGAGCATAACAAGAACCACGCGACTGAGAACTGTTGACCGAAAACCAAAACAAAGAGAAACGAAAAGTTCTGTTCAGAGCGAGAGCACACGTGCA--TCGTCTTTTTCGGTTGTGTTCGAGTGCAGACCGCAAAA--CGAAAAACGGTTGACAGTTATTTGCGAGCTATGCTCTGTAGGGGTCATGCAACTCAAA-GA---------TAGATCTTTAATGATTTAAGATTAGGGGTTAG------TAGTTTCTAGTAAATCT--------	Size	Hit Count	Total Norm	Total	M026 Head	M043 Female-body	M056 Embryo	V046 Embryo	V052 Head	V058 Head	V120 Male-body	SRR1275488 Male larvae	SRR1275486 Male prepupae	SRR1275484 Male prepupae	GSM1528802 follicle cells
.....................................................AGAGAAACGAAAAGTTCTGTTCAGAGC..............................................................................................................................................................................................	27	12	7.58	91	0	8	68	0	0	0	1	0	0	0	14
....CATAACAAGAACCACGCGACTGAGAA................................................................................................................................................................................................................................................	26	6	3.50	21	0	6	8	0	0	0	0	0	0	0	7
......................................................GAGAAACGAAAAGTTCTGTTCAGAGC..............................................................................................................................................................................................	26	12	2.08	25	0	1	9	0	0	0	0	0	0	0	15
............................................................................................................................................AAAACGGTTGACAGTTATTTGCGAT.........................................................................................................	25	9	1.56	14	0	0	0	0	0	0	0	0	0	0	14
TGAGCATAACAAGAACCACGCGACTGA...................................................................................................................................................................................................................................................	27	7	0.86	6	0	4	2	0	0	0	0	0	0	0	0
....CATAACAAGAACCACGCGACTGAGA.................................................................................................................................................................................................................................................	25	6	0.83	5	0	5	0	0	0	0	0	0	0	0	0
....CATAACAAGAACCACGCGACTGAGAACT..............................................................................................................................................................................................................................................	28	3	0.67	2	0	1	1	0	0	0	0	0	0	0	0
..................................................CAAAGAGAAACGAAAAGTTCTGTT....................................................................................................................................................................................................	24	12	0.67	8	0	0	8	0	0	0	0	0	0	0	0
...................................................................................................................................................TTGACAGTTATTTGCGAGCTATG....................................................................................................	23	10	0.60	6	0	4	2	0	0	0	0	0	0	0	0
.....................................................AGAGAAACGAAAAGTTCTGTTCAGAG...............................................................................................................................................................................................	26	12	0.58	7	0	4	3	0	0	0	0	0	0	0	0
..AGCATAACAAGAACCACGCGACTGA...................................................................................................................................................................................................................................................	25	7	0.57	4	0	4	0	0	0	0	0	0	0	0	0
......TAACAAGAACCACGCGACTGAGAACT..............................................................................................................................................................................................................................................	26	4	0.50	2	0	2	0	0	0	0	0	0	0	0	0
.....................................................AGAGAAACGAAAAGTTCTGTTCAGA................................................................................................................................................................................................	25	12	0.50	6	0	2	0	0	0	0	0	0	0	0	4
TGAGCATAACAAGAACCACGCGAC......................................................................................................................................................................................................................................................	24	7	0.43	3	0	2	1	0	0	0	0	0	0	0	0
......TAACAAGAACCACGCGACTGAGAA................................................................................................................................................................................................................................................	24	7	0.43	3	0	0	3	0	0	0	0	0	0	0	0
..AGCATAACAAGAACCACGCGACTGAGAA................................................................................................................................................................................................................................................	28	6	0.33	2	1	1	0	0	0	0	0	0	0	0	0
..AGCATAACAAGAACCACGCGACTGAG..................................................................................................................................................................................................................................................	26	6	0.33	2	0	2	0	0	0	0	0	0	0	0	0
....CATAACAAGAACCACGCGACTGAGAAC...............................................................................................................................................................................................................................................	27	6	0.33	2	0	2	0	0	0	0	0	0	0	0	0
.GAGCATAACAAGAACCACGCGACT.....................................................................................................................................................................................................................................................	24	7	0.29	2	0	1	1	0	0	0	0	0	0	0	0
..AGCATAACAAGAACCACGCGAC......................................................................................................................................................................................................................................................	22	7	0.29	2	0	2	0	0	0	0	0	0	0	0	0
..AGCATAACAAGAACCACGCGACTG....................................................................................................................................................................................................................................................	24	7	0.29	2	0	2	0	0	0	0	0	0	0	0	0
....CATAACAAGAACCACGCGACTGA...................................................................................................................................................................................................................................................	23	7	0.29	2	0	2	0	0	0	0	0	0	0	0	0
..AGCATAACAAGAACCACGCGACT.....................................................................................................................................................................................................................................................	23	7	0.29	2	0	2	0	0	0	0	0	0	0	0	0
................................................................AAGTTCTGTTCAGAGCGAGAGCACA.....................................................................................................................................................................................	25	11	0.27	3	0	0	2	1	0	0	0	0	0	0	0
......TAACAAGAACCACGCGACTGAGAACTGT............................................................................................................................................................................................................................................	28	4	0.25	1	0	1	0	0	0	0	0	0	0	0	0
......TAACAAGAACCACGCGACTGAGAACTG.............................................................................................................................................................................................................................................	27	4	0.25	1	0	1	0	0	0	0	0	0	0	0	0
..............................................................................................................................................AACGGTTGACAGTTATTTGCGAGC........................................................................................................	24	9	0.22	2	0	0	2	0	0	0	0	0	0	0	0
...................................................................................................................TTCGAGTGCAGACCGCAAAA--CGA..................................................................................................................................	23	9	0.22	2	0	2	0	0	0	0	0	0	0	0	0
....................................................................................................................................................TGACAGTTATTTGCGAGCTATG....................................................................................................	22	10	0.20	2	0	2	0	0	0	0	0	0	0	0	0
...................................................................................................................TTCGAGTGCAGACCGCAAAA--C....................................................................................................................................	21	10	0.20	2	0	2	0	0	0	0	0	0	0	0	0
...................................................................................................................................................TTGACAGTTATTTGCGAGCTA......................................................................................................	21	10	0.20	2	0	1	0	0	0	0	1	0	0	0	0
...............................................................................................................TGTGTTCGAGTGCAGACCGCAAAA--CG...................................................................................................................................	26	10	0.20	2	0	2	0	0	0	0	0	0	0	0	0
...................................................................................AGCACACGTGCA--TCGTCTTTTTCGGTTG.............................................................................................................................................................	28	5	0.20	1	0	1	0	0	0	0	0	0	0	0	0
................................GTTGACCGAAAACCAAAACAAAGAGA....................................................................................................................................................................................................................	26	11	0.18	2	0	1	1	0	0	0	0	0	0	0	0
TGAGCATAACAAGAACCACGCGACTGAG..................................................................................................................................................................................................................................................	28	6	0.17	1	0	1	0	0	0	0	0	0	0	0	0
..AGCATAACAAGAACCACGCGACTGAGA.................................................................................................................................................................................................................................................	27	6	0.17	1	0	1	0	0	0	0	0	0	0	0	0
................................................AACAAAGAGAAACGAAAAGTTCTGTT....................................................................................................................................................................................................	26	12	0.17	2	0	0	2	0	0	0	0	0	0	0	0
......................................................GAGAAACGAAAAGTTCTGTTCAGAG...............................................................................................................................................................................................	25	12	0.17	2	0	1	1	0	0	0	0	0	0	0	0
.....................................................AGAGAAACGAAAAGTTCTGTTCAGAGT..............................................................................................................................................................................................	27	12	0.17	2	0	0	1	0	0	0	0	0	0	0	1
...........................................................................AGAGCGAGAGCACACGTGCA--T............................................................................................................................................................................	21	6	0.17	1	0	0	1	0	0	0	0	0	0	0	0
..................................................................................................................................................GTTGACAGTTATTTG.............................................................................................................	15	12	0.17	2	0	0	0	0	0	0	0	0	0	0	2
.GAGCATAACAAGAACCACGCGACTGAGA.................................................................................................................................................................................................................................................	28	6	0.17	1	0	0	1	0	0	0	0	0	0	0	0
....CATAACAAGAACCACGCGACTG....................................................................................................................................................................................................................................................	22	7	0.14	1	0	1	0	0	0	0	0	0	0	0	0
..AGCATAACAAGAACCACGCGA.......................................................................................................................................................................................................................................................	21	7	0.14	1	0	0	1	0	0	0	0	0	0	0	0
TGAGCATAACAAGAACCACGCGACT.....................................................................................................................................................................................................................................................	25	7	0.14	1	0	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................GCAAAA--CGAAAAACGGTTGACAGTTATT...............................................................................................................	28	7	0.14	1	0	1	0	0	0	0	0	0	0	0	0
......TAACAAGAACCACGCGACTGAGAAC...............................................................................................................................................................................................................................................	25	7	0.14	1	0	0	1	0	0	0	0	0	0	0	0
......TAACAAGAACCACGCGACTGAG..................................................................................................................................................................................................................................................	22	7	0.14	1	0	0	1	0	0	0	0	0	0	0	0
....CATAACAAGAACCACGCGACT.....................................................................................................................................................................................................................................................	21	7	0.14	1	0	1	0	0	0	0	0	0	0	0	0
.GAGCATAACAAGAACCACGCGAC......................................................................................................................................................................................................................................................	23	7	0.14	1	0	1	0	0	0	0	0	0	0	0	0
....................................................................................................................................AAA--CGAAAAACGGTTGACAGTTATTT..............................................................................................................	26	8	0.13	1	0	0	1	0	0	0	0	0	0	0	0
.........................................................................................................................TGCAGACCGCAAAA--CGAAAAACGG...........................................................................................................................	24	8	0.13	1	0	1	0	0	0	0	0	0	0	0	0
......TAACAAGAACCACGCGACT.....................................................................................................................................................................................................................................................	19	8	0.13	1	0	1	0	0	0	0	0	0	0	0	0
.................................................................................................................TGTTCGAGTGCAGACCGCAAAA--CGA..................................................................................................................................	25	9	0.11	1	0	1	0	0	0	0	0	0	0	0	0
.............................................................................................................................................AAACGGTTGACAGTTATTTGCGAGCT.......................................................................................................	26	9	0.11	1	0	0	1	0	0	0	0	0	0	0	0
............................................................................................................................................AAAACGGTTGACAGTTATTTGCGGT.........................................................................................................	25	9	0.11	1	0	0	0	0	0	0	0	0	0	0	1
.............................................................................................................................................AAACGGTTGACAGTTATTTGCGAGC........................................................................................................	25	9	0.11	1	0	0	1	0	0	0	0	0	0	0	0
....................................................................................................................................................TGACAGTTATTTGCGAGCT.......................................................................................................	19	10	0.10	1	0	0	1	0	0	0	0	0	0	0	0
...................................................................................................................................................TTGACAGTTATTTGCGAGCT.......................................................................................................	20	10	0.10	1	0	1	0	0	0	0	0	0	0	0	0
.......................................................................................................TTTTCGGTTGTGTTCGAGTGCAGACC.............................................................................................................................................	26	10	0.10	1	0	0	1	0	0	0	0	0	0	0	0
...............................................................................................................TGTGTTCGAGTGCAGACCGCAAAA--C....................................................................................................................................	25	10	0.10	1	0	0	1	0	0	0	0	0	0	0	0
...................................................................................................................................................TTGACAGTTATTTGCGAGCTAT.....................................................................................................	22	10	0.10	1	0	1	0	0	0	0	0	0	0	0	0
...............................................................................................................................................ACGGTTGACAGTTATTTGCGAG.........................................................................................................	22	10	0.10	1	0	0	0	0	0	0	1	0	0	0	0
.......................................................................................................TTTTCGGTTGTGTTCGAGTGCAGA...............................................................................................................................................	24	10	0.10	1	0	0	1	0	0	0	0	0	0	0	0
...................................................................................................................TTCGAGTGCAGACCGCAAAA--CA...................................................................................................................................	22	10	0.10	1	0	1	0	0	0	0	0	0	0	0	0
....................................................................................................................TCGAGTGCAGACCGCAAAA--CG...................................................................................................................................	21	10	0.10	1	0	1	0	0	0	0	0	0	0	0	0
........................................................................................................TTTCGGTTGTGTTCGAGTGCAGACC.............................................................................................................................................	25	10	0.10	1	0	0	1	0	0	0	0	0	0	0	0
......................................................................................................TTTTTCGGTTGTGTTCGAGTGCAG................................................................................................................................................	24	10	0.10	1	0	1	0	0	0	0	0	0	0	0	0
...............................................................................................................................................ACGGTTGACAGTTATTTGCGAGC........................................................................................................	23	10	0.10	1	0	0	1	0	0	0	0	0	0	0	0
......................................................................................................TTTTTCGGTTGTGTTCGAGTGCAGAC..............................................................................................................................................	26	10	0.10	1	0	0	1	0	0	0	0	0	0	0	0
.................................................................AGTTCTGTTCAGAGCGAGAGCACA.....................................................................................................................................................................................	24	11	0.09	1	0	1	0	0	0	0	0	0	0	0	0
..................................TGACCGAAAACCAAAACAAAGAGAAA..................................................................................................................................................................................................................	26	11	0.09	1	0	0	1	0	0	0	0	0	0	0	0
...................................................................TTCTGTTCAGAGCGAGAGCACACG...................................................................................................................................................................................	24	11	0.09	1	0	0	1	0	0	0	0	0	0	0	0
..................................TGACCGAAAACCAAAACAAAGAGAAAC.................................................................................................................................................................................................................	27	11	0.09	1	0	1	0	0	0	0	0	0	0	0	0
.................................TTGACCGAAAACCAAAACAAAA.......................................................................................................................................................................................................................	22	11	0.09	1	0	0	1	0	0	0	0	0	0	0	0
.................................TTGACCGAAAACCAAAACAAAGAGAAAC.................................................................................................................................................................................................................	28	11	0.09	1	0	0	1	0	0	0	0	0	0	0	0
...............................................................AAAGTTCTGTTCAGAGCGAGAGCACACG...................................................................................................................................................................................	28	11	0.09	1	0	1	0	0	0	0	0	0	0	0	0
..................................TGACCGAAAACCAAAACAAAGAGAAACGA...............................................................................................................................................................................................................	29	11	0.09	1	0	0	1	0	0	0	0	0	0	0	0
.........................................................................................................TTCGGTTGTGTTCGAGTGCAGA...............................................................................................................................................	22	11	0.09	1	0	0	1	0	0	0	0	0	0	0	0
......................................................GAGAAACGAAAAGTTCTGTTCAGA................................................................................................................................................................................................	24	12	0.08	1	0	1	0	0	0	0	0	0	0	0	0
......................................................GAGAAACGAAAAGTTCTGTTCAGAGCG.............................................................................................................................................................................................	27	12	0.08	1	0	1	0	0	0	0	0	0	0	0	0
.................................................ACAAAGAGAAACGAAAAGTTCTGTT....................................................................................................................................................................................................	25	12	0.08	1	0	0	1	0	0	0	0	0	0	0	0
..........................................AACCAAAACAAAGAGAAACGAAAAGTT.........................................................................................................................................................................................................	27	12	0.08	1	0	1	0	0	0	0	0	0	0	0	0
...............................................................AAAGTTCTGTTCAGAGCGAGAGCAC......................................................................................................................................................................................	25	12	0.08	1	0	0	1	0	0	0	0	0	0	0	0
......................................................GAGAAACGAAAAGTTCTGTTCAGAGCGA............................................................................................................................................................................................	28	12	0.08	1	0	0	1	0	0	0	0	0	0	0	0
.....................................................AGAGAAACGAAAAGTTCTGTTCAGACT..............................................................................................................................................................................................	27	12	0.08	1	0	0	1	0	0	0	0	0	0	0	0
....................................................AAGAGAAACGAAAAGTTCTGTTCAGAGC..............................................................................................................................................................................................	28	12	0.08	1	0	0	1	0	0	0	0	0	0	0	0
.....................................................AGAGAAACGAAAAGTTCTGTTCAG.................................................................................................................................................................................................	24	13	0.08	1	0	0	1	0	0	0	0	0	0	0	0
............................................................CGAAAAGTTCTGTTCAGAGC..............................................................................................................................................................................................	20	13	0.08	1	0	1	0	0	0	0	0	0	0	0	0
.........................................................AAACGAAAAGTTCTGTTCAGAGC..............................................................................................................................................................................................	23	13	0.08	1	0	1	0	0	0	0	0	0	0	0	0
.................................TTGACCGAAAACCAAAACAAAAT......................................................................................................................................................................................................................	23	14	0.07	1	0	0	1	0	0	0	0	0	0	0	0

droEre2

TAGTCGC------------------------------------------------------------------------------------------------------------------------------------------------TTCTTATTGGTGATCTATTAC-----------------------------------------------------------------------CAAATCCATTAGCAAATTTATCA--AT	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
No Reads

dwi_2464

scf2_1100000004902:3390151-3390301 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-35.1	-35.0	-34.9