dwi_2186

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------################################################## GTTATTTCCTATGCTACGATTGCTGGCCGTTCTGTCGCAAACATTAGCCTTTTTAGATAAAACGTTTTTCCACTTTGATGGCTATAGGTAAGGAAAGAGTTACCAAAAATGTTGCAAGGGTATACAAACTTTGACGCGGTCGAAGTTAGCCCCGGCCCTCTGGTTAAAATTAAGTTTTAATAACGTTTGTTAACCTTCTAGACCCATTGAGAAAACTTGGACCTACAAATGTGAGAACGATCGTTGCATTC *************************************************************************..........................................((((......((((((((...))))))))..))))*************************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M045 female body	V119 head	V118 embryo
................................................................................................................................CTTTGACGCGGTCGAAGTTAGCCC...................................................................................................	24	0	20	0.25	5	5	0	0
...........................................................................................................................................................................................................................GTCCAACAAATGTGAGAGCGA...........	21	3	4	0.25	1	1	0	0
..................................................................................................................................................................................................................................AAAGGTGCGAACGATGGTTGC....	21	3	4	0.25	1	1	0	0
...........................................................................TGATGGCTATAGGTAAGGAAAG..........................................................................................................................................................	22	0	20	0.20	4	0	4	0
............................................................................................................................................CGAAGTTAGCCCCGGCCCTCTGGTTAAA...................................................................................	28	0	19	0.05	1	1	0	0
.....TTCCTATGCTACGATTGCTGGCCGT.............................................................................................................................................................................................................................	25	0	20	0.05	1	0	0	1
...................................................TTTAGATAAAACGTTTTTCCACTTTGA.............................................................................................................................................................................	27	0	20	0.05	1	0	0	1
.....................................................TAGATAAAACGTTTTTCCACTTTGA.............................................................................................................................................................................	25	0	20	0.05	1	0	0	1
.........TATGCTACGATTGCTGGCCGT.............................................................................................................................................................................................................................	21	0	20	0.05	1	1	0	0

CAATAAAGGATACGATGCTAACGACCGGCAAGACAGCGTTTGTAATCGGAAAAATCTATTTTGCAAAAAGGTGAAACTACCGATATCCATTCCTTTCTCAATGGTTTTTACAACGTTCCCATATGTTTGAAACTGCGCCAGCTTCAATCGGGGCCGGGAGACCAATTTTAATTCAAAATTATTGCAAACAATTGGAAGATCTGGGTAACTCTTTTGAACCTGGATGTTTACACTCTTGCTAGCAACGTAAG *************************************************************************************************..........................................((((......((((((((...))))))))..))))*************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M045 female body	V118 embryo	V117 male body
......................................................................................................................................GCGCCAGCTTCAATCGGGGCC................................................................................................	21	0	20	0.20	4	4	0	0
.....................................................................................................................................................................................TCGCAAATAGTTGGAAGAT...................................................	19	3	20	0.10	2	0	2	0
................................ACAGCGTTTGTAATCGGAAAAATCTAT................................................................................................................................................................................................	27	0	18	0.06	1	1	0	0
........................................TGTAATCGGAAAAATCTAT................................................................................................................................................................................................	19	0	20	0.05	1	0	0	1
......................GACCGGCAAGACAGCGTTTGT................................................................................................................................................................................................................	21	0	20	0.05	1	1	0	0
..............................................................................................................CAACGTTCCCATATGTTTGAAAC......................................................................................................................	23	0	20	0.05	1	1	0	0
.............GATGCTAACGACCGGCAAGACA........................................................................................................................................................................................................................	22	0	20	0.05	1	1	0	0
.............................................TCGGAAAAATCTATTTTGCAAAAAGG....................................................................................................................................................................................	26	0	20	0.05	1	1	0	0
...................................................AAATCTATTTTGCAAAAAGGTGAAACT.............................................................................................................................................................................	27	0	20	0.05	1	0	1	0
............................................................................................................................GTTTGAAACTGCGCCAGCTTCAATC......................................................................................................	25	0	20	0.05	1	0	1	0
......AGGATACGATGCTAACGACCGGC..............................................................................................................................................................................................................................	23	0	20	0.05	1	0	1	0
..............ATGCTAACGACCGGCAAGACAGCGT....................................................................................................................................................................................................................	25	0	20	0.05	1	1	0	0

Species	Coordinate	ID	Alignment
droWil2	scf2_1100000004822:1923767-1924017 -	dwi_2186	GTTATTTCCTATGCTACGAT--TGCTGGCCGTTCTGTCGCAAA-CATTAGCCTTTTTAGATAAAACGTTTTTCCACTTTGAT---------------------------------------------------------------------------------------GGC----------------------------------TATAGGTAAGGAAAGAGTTA----------------------C----------CA-----------AAA----ATGTTGCAAGGGTATACAAACTTTGACGC-GGTCGAAGTTAGCCC-CGGCCCTCTGGTTAA--A-A-----------------TTAAGTTTTAATAA------------CGTTTGTTA-----ACCTTC-------------------------------------------TAGACCCATTGAGA--------------------AAACTTGGACC----TACAA-------------ATGTGAGAACGATCGTTGCATTC
droVir3	scaffold_13045:248210-248476 -		ATTTTGTTTATCA-AG--ATATCTT-GACC----AAACTCGGCATT---------------------------------------------------------------------TACTATTTTCCCGGTACTTCTTAGATAGAGGCAAAGCACAATGAGCATTATGAAAA-----------------------------------------------------------------------------------------------------GGTTG--GGTCTGCAAGGGTATTAGATCTTTGGCGT--GCTGAAGATAGCCCTTCTTTC--TCGTTTT--ATAGTAACTTAACGTGTTAA-----------AC--TTAAATATATA-----TATA----------------------------------------TCTTTGCATACCTCTT-----ATAATTCATTTTTAAACCCTGAACCCA----TTAA--A----ATTGA-------------GTATAAGG--GTATATTGTATTT
droMoj3	scaffold_6496:235046-235136 +		TAGGTAC-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------A-A-----------------TTAAATTAAACGAA------------TTTCTTCTC-----TTTTCT-------------------------------------------TAGACCGCTGGAAA--------------------AGTCATGGACC----TATAA-------------ATGCGACAATGACAGATGTGTAC
droGri2	scaffold_15245:6240771-6240831 -		AACGTTTG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAGACCATTGGAGA--------------------AATCGTGGACC----TACAA-------------ATGCGAGAATGACAGATGCGTGC
dp5	3:17313575-17313630 -		TTA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAGACCCGTGGAGA--------------------AGTTTTGGACA----TACAA-------------ATGCGAGAATGACCGATGTATGC
droPer2	scaffold_31:477182-477237 +		TTA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAGACCCGTGGAGA--------------------AGTTTTGGACA----TACAA-------------ATGCGAGAATGACCGATGTATGC
droAna3	scaffold_13165:359055-359323 +		GTTGTTTTTCAAGTTAGAAG--CATGGGAG-----------------------------TTTCAATGGA-----------------TTCCTCTTTTGGCA---------------AAATAAT-TCGATA----------------------------------------TGCG----ATATTATTCGA---------------------------------------TATT--CGATAATATAATATGCGTGGCGCCACCT-AGCGGACTGCGAC-T--CAACTGCAAGGGTATATCAACTTCGGCTCCGCCCGAAGTTAGCTTTCCTTTC--TTGTTAA--TTG-------T-------AA-----------TATT--GT-TATTTA-----TGTA----------------------------------------ATATTGTATACTTGTGAATAAATTGAAA--------------------ATTTTTGGAAC----TATAC-------------ATTA---ATCGAACG--TCATTC
droBip1	scf7180000395791:384-669 +		GTATTTTTGTAAGATAGAAG--TTCGGGACG-----------------------------T----------------TTTTTTAGTATTTGTTATTG-TA----ATAAATTGGATTATATAT-TCATAT----------------------------------------TCCCATAAGGATCGGCCAACTATATCCGATGTTTGTC----GA---------------T----------ATATAATCCG------------------------GTTTT--AACTTGCAAGGGTATATAAACTTCAGCTCCGCCCGAAGTTAGCTTTCCTTTC--TTGTTTT--TTT-------T-------AA-----------AC--TTCAATTA------------ACATATTTCATAAATAA-----------------ACTTTA------------------TCTAAAAAGG--------------------TT---A--GAA----TGTAATTTGGAATCATCGGTATCAGAAAGAGCCTTATATAT
droKik1	scf7180000300249:160024-160277 +		AAAGGTGTGAATG-TATAAC--TGT-AACTGTCAAACTGTAAA-CATAA-TAAGTATAGGTAAAATGTTATGAAACTCTGTTTAGTGTCTGTTTTCGGCA---------------T------------------------------------------------------------------------------------------TATAATTTATGATATAAATA----------------------T----------GA-----------AAACC--AATCTGGAAGGGTATACAAACTTCGGCGT--GCCGAAGTTAGCTT-CCTTTC--TTGTTTT--TTATTAATTTT-------AA-----------GATT--AC-TAATTA------------------------------------------------------------------AACAACAAAAT--------------------AATAAATGAAA----CACAA-------------CTTTGAGATCGTCCGTTCCTTGC
droFic1	scf7180000453815:110874-111092 -		GTCATAG----------------------------------------------------CTTCGATGTT-----------------GT------TTAGCC---------------------T-TTATGT----------------------------------------ACCTATATATTTTGTTCGA--------------------------------CATA-----TT-----------------------------------------TGA----CGACTGCAAGGGTATATCAGCTTTGGCTT--GCCGAAGCTAGCTT-TCTTTC--TTGTTAA--TAA-------T-------AG-----------TTTT--GA-TAA---TAGAATTTAAC---ACTGTCTAGACACGACACTGTGGCAGA-------------T--------TAAGATCATG-TAT--------------------AAATTTAGTTT----TAAAA-------------AGGTAGAGAAAAGCGTTATA---
droEle1	scf7180000491268:77162-77401 +		CTATCTTATA--------AT--TG----------AGCTGCAAATCATC-------ATAGTTTCAATGTT-----------------TT------TCAGCATATACTCAAGT--------------------------------------------------------------------------------------------------AAATTATAATTTAAATATT-TTCTAAAGTATTTAATTCAT-----------G-----------CAA----TAGCTGCAAGGGTATATGAACTTCGGCTT--GCCGAAGTTTGCTT-TCTTTC--TTGTTATCGTTT-------T-------AA-----------TAG-TTTGATAA------------ATA------------CTCATCGTTA-------AAAGTTTATTA------------------------------------------------TTGTGAGGTACAAGTGA---------------TTGAGATCAA-TTTGGCAGTT
droRho1	scf7180000779834:10188-10414 -		GGTGTTTTTAAAGTTAGGAA--GATGGGATT-----------------------------T----------------CTTA---------------------------------------------------------------------------TGAGTATTAAGAATGC------------------------------------------ATTATATGAATAATTTT--GGAGAATATAATTTAC-----------A-----------GAA-T--ATTCTGCAAGGGTATATCAACTTCGGCTCCGCCCGAAGTTAGCTTTCCTTTC--TTGTTGT--TAA-------T-------AA-----------AT--ACGT-TT--------TC---CCCAAAATCTCAAATCT-----------------TGATTC------------------TCTGCAAAAA--------------------AG---T--AAA----CGTAA-------------ATGAGAGAATAGTAACTACA---
droBia1	scf7180000300353:831-1039 -		TTTATTTCTTATA-TATTAT--CTTAGGCC----AGCAACAATCCT---------------------------------------------------------------------TAAAAATTTC--------------------------------------------------------------------------------------------------------------------------ACATGGTGTTATTAAAGTTGACTATTTC-TTGTAACTGCAAGGGTATACAAACTTCGGCTT--GCCGAAGCTAACTT-CCTTTC--TTGTTATGGTAA-------T-------CA-----------AT--TCAG-TT--------TTCTA-----ATTTTCTAGTCTCTCCG---------------------------------------------------------------------TTTGGCTT----TGATT-------------ATGAAACAGCGATTGATGGATT-
droTak1	scf7180000415388:90061-90250 -		ATTTTTTTCAATTATAGAAT--ATTTTGTTGT-------------T---------------------------------------------------------------------TATTAAT-TCATGT----------------------------------------TGCT----ATTTTAGTCAA------------------------------------------------------------------------------------GTT----TTCAAGCAAGGGTATTAAAACTTCGGCTT--GCCGAAGTTAGCTT-CCGTCC--TCGTTTT--TAA-------A-------AA-----------TATT--AC-CT----------------------TC-------------------------------------------C---TCCATTGGGAATTATTATACCCGTTACTCG------------------TA-------------GAGTAAAAGGGTACATTGTATTC
droEug1	scf7180000409462:1730259-1730314 +		TTG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAGACCCGTCGAGA--------------------AGTCCTGGACT----TACAA-------------GTGCGAGGATAAGCGATGTATAC
dm3	chr2R:8377910-8377961 -		A--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GACCCGTCGAGA--------------------AGTCCTGGACG----TACAA-------------GTGCGAGAATGACCGATGCATGC
droSim2	2r:9087834-9087890 -		CTTG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAGACCCGTCGAGA--------------------AGTCCTGGACG----TACAA-------------GTGCGAGAATGACCGATGCATGC
droSec2	scaffold_1:5681313-5681372 +		CA------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAA----CTACTGCAAGGGCATACAAACTTCGGCTT--GTCGCAGTTAATTT-CCCTTC--CTGTTA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droYak3	2R:8558440-8558496 +		CTTG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAGACCCGTCGAGA--------------------AGTCCTGGACG----TACAA-------------GTGCGAGAATGACCGATGCATGC
droEre2	scaffold_4845:17412632-17412688 +		CTTG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAGACCCGTCGAGA--------------------AGTCCTGGACA----TACAA-------------GTGCGAGAATGACCGATGCATCC

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droWil2

GTTATTTCCTATGCTACGAT--TGCTGGCCGTTCTGTCGCAAA-CATTAGCCTTTTTAGATAAAACGTTTTTCCACTTTGAT---------------------------------------------------------------------------------------GGC----------------------------------TATAGGTAAGGAAAGAGTTA----------------------C----------CA-----------AAA----ATGTTGCAAGGGTATACAAACTTTGACGC-GGTCGAAGTTAGCCC-CGGCCCTCTGGTTAA--A-A-----------------TTAAGTTTTAATAA------------CGTTTGTTA-----ACCTTC-------------------------------------------TAGACCCATTGAGA--------------------AAACTTGGACC----TACAA-------------ATGTGAGAACGATCGTTGCATTC	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
...........................................................................................................................................................................................................................................................................................................CTTTGACGC-GGTCGAAGTTAGCCC-........................................................................................................................................................................................................................	24	20	0.25	5	0	5	0	0	0
..............................................................................TGAT---------------------------------------------------------------------------------------GGC----------------------------------TATAGGTAAGGAAAG................................................................................................................................................................................................................................................................................................................................	22	20	0.20	4	0	0	0	0	4
........................................................................................................................................................................................................................................................................................................................CGAAGTTAGCCC-CGGCCCTCTGGTTAA--A-.....................................................................................................................................................................................................	28	19	0.05	1	0	1	0	0	0
.....TTCCTATGCTACGAT--TGCTGGCCGT.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	25	20	0.05	1	0	0	0	1	0
......................................................TTTAGATAAAACGTTTTTCCACTTTGA............................................................................................................................................................................................................................................................................................................................................................................................................................................................................	27	20	0.05	1	0	0	0	1	0
........................................................TAGATAAAACGTTTTTCCACTTTGA............................................................................................................................................................................................................................................................................................................................................................................................................................................................................	25	20	0.05	1	0	0	0	1	0
.........TATGCTACGAT--TGCTGGCCGT.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	20	0.05	1	0	1	0	0	0

droVir3

ATTTTGTTTATCA-AG--ATATCTT-GACC----AAACTCGGCATT---------------------------------------------------------------------TACTATTTTCCCGGTACTTCTTAGATAGAGGCAAAGCACAATGAGCATTATGAAAA-----------------------------------------------------------------------------------------------------GGTTG--GGTCTGCAAGGGTATTAGATCTTTGGCGT--GCTGAAGATAGCCCTTCTTTC--TCGTTTT--ATAGTAACTTAACGTGTTAA-----------AC--TTAAATATATA-----TATA----------------------------------------TCTTTGCATACCTCTT-----ATAATTCATTTTTAAACCCTGAACCCA----TTAA--A----ATTGA-------------GTATAAGG--GTATATTGTATTT	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
............................................................................................................................................................TGAGCATTATGAAAA-----------------------------------------------------------------------------------------------------GGTTG--GGTCTGC...............................................................................................................................................................................................................................................................	27	20	0.90	18	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	18	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..........TCA-AG--ATATCTT-GACC----AAACTCGGCA.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	20	0.65	13	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	13	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................................................................................................................................................................................................................................................................................TGCAAGGGTATTAGATCTTTGGC...........................................................................................................................................................................................................................................	23	20	0.55	11	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	11	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................................................................................................ATTAGATCTTTGGCGT--GCTGAAGA...............................................................................................................................................................................................................................	24	8	0.25	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................AGCATTATGAAAA-----------------------------------------------------------------------------------------------------GGTTG--GGT...................................................................................................................................................................................................................................................................	21	20	0.20	4	0	0	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................................................................................................................T--GCTGAAGATAGCCCTTCT.....................................................................................................................................................................................................................	19	6	0.17	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................................................................................................TAGATCTTTGGCGT--GCTGAAGATA.............................................................................................................................................................................................................................	24	6	0.17	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................AGCATTATGAAAA-----------------------------------------------------------------------------------------------------GGTTG--GGTCTGC...............................................................................................................................................................................................................................................................	25	20	0.15	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................................................................................................................AATGAGCATTATGAAAA-----------------------------------------------------------------------------------------------------GGTTG--......................................................................................................................................................................................................................................................................	22	8	0.13	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................................................................................................TATTAGATCTTTGGCGT--GCTGAAGAT..............................................................................................................................................................................................................................	26	8	0.13	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................................................................................................................TCCCGGTACTTCTTAGATAGAGGCAAA.......................................................................................................................................................................................................................................................................................................................................................................................................	27	20	0.10	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................................................................................................................TCCCGGTACTTCTTAGATAGAGGCAA........................................................................................................................................................................................................................................................................................................................................................................................................	26	20	0.10	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................TCTTAGATAGAGGCAAAGC.....................................................................................................................................................................................................................................................................................................................................................................................................	19	20	0.10	2	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................GCATTATGAAAA-----------------------------------------------------------------------------------------------------GGTTG--GGTCTGC...............................................................................................................................................................................................................................................................	24	20	0.10	2	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................TT---------------------------------------------------------------------TACTATTTTCCCGGTACTTCTTAGA.................................................................................................................................................................................................................................................................................................................................................................................................................	27	20	0.10	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................TGAAAA-----------------------------------------------------------------------------------------------------GGTTG--GGTCTGCAAGGGTAT.......................................................................................................................................................................................................................................................	26	20	0.10	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................................................................................................ATTATGAAAA-----------------------------------------------------------------------------------------------------GGTTG--GGTC..................................................................................................................................................................................................................................................................	19	20	0.10	2	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................GCATTATGAAAA-----------------------------------------------------------------------------------------------------GGTTG--GGTC..................................................................................................................................................................................................................................................................	21	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................TGAAAA-----------------------------------------------------------------------------------------------------GGTTG--GGTCTGCAAG............................................................................................................................................................................................................................................................	21	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................................................................................................................................................................................................................................................................................TGCAAGGGTATTAGATCTTTGG............................................................................................................................................................................................................................................	22	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
................................................................................................................................................................CATTATGAAAA-----------------------------------------------------------------------------------------------------GGTTG--GGTCTGC...............................................................................................................................................................................................................................................................	23	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................................................................................................................TCCCGGTACTTCTTAGATAGAGGC..........................................................................................................................................................................................................................................................................................................................................................................................................	24	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................AAAA-----------------------------------------------------------------------------------------------------GGTTG--GGTCTGCAAGGGTA........................................................................................................................................................................................................................................................	23	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................................................................................................................TCTGCAAGGGTATTAGATCTTTGGCG..........................................................................................................................................................................................................................................	26	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................................................................................TG--GGTCTGCAAGGGTATTAGATC.................................................................................................................................................................................................................................................	23	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................................................................................................................................................GAGCATTATGAAAA-----------------------------------------------------------------------------------------------------GGTTG--GGTCT.................................................................................................................................................................................................................................................................	24	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTTTAAACCCTGAACC..........................................................	17	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................................................................................TTCCCGGTACTTCTTAGATAGAGGCAAA.......................................................................................................................................................................................................................................................................................................................................................................................................	28	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................................................................TTG--GGTCTGCAAGGGTATTAGATCTT...............................................................................................................................................................................................................................................	26	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................................................................TATTTTCCCGGTACTTCTTAGAT................................................................................................................................................................................................................................................................................................................................................................................................................	23	20	0.05	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................................................................................TTCCCGGTACTTCTTAGATAGAG............................................................................................................................................................................................................................................................................................................................................................................................................	23	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................CC----AAACTCGGCATT---------------------------------------------------------------------TACTATTTTCC...............................................................................................................................................................................................................................................................................................................................................................................................................................	25	20	0.05	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................................................TTTCCCGGTACTTCTTAGATAGAGGCA.........................................................................................................................................................................................................................................................................................................................................................................................................	27	20	0.05	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................................................................................................................TCCCGGTACTTCTTAGATAGAGG...........................................................................................................................................................................................................................................................................................................................................................................................................	23	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................TGAAAA-----------------------------------------------------------------------------------------------------GGTTG--GGTCTGCAAGGGTA........................................................................................................................................................................................................................................................	25	20	0.05	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......TTTATCA-AG--ATATCTT-GACC----AAACTC.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................AGCATTATGAAAA-----------------------------------------------------------------------------------------------------GGTTG--GGTCTGCAA.............................................................................................................................................................................................................................................................	27	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................C----AAACTCGGCATT---------------------------------------------------------------------TACTATTTTCCCGGT...........................................................................................................................................................................................................................................................................................................................................................................................................................	28	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................................................................................................................TCTGCAAGGGTATTAGATCTTTG.............................................................................................................................................................................................................................................	23	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
........................................................................................................................TTTTCCCGGTACTTCTTAGATAGAGGCA.........................................................................................................................................................................................................................................................................................................................................................................................................	28	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droMoj3

TAGGTAC-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------A-A-----------------TTAAATTAAACGAA------------TTTCTTCTC-----TTTTCT-------------------------------------------TAGACCGCTGGAAA--------------------AGTCATGGACC----TATAA-------------ATGCGACAATGACAGATGTGTAC	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
No Reads

droGri2

AACGTTTG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAGACCATTGGAGA--------------------AATCGTGGACC----TACAA-------------ATGCGAGAATGACAGATGCGTGC	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

dp5

TTA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAGACCCGTGGAGA--------------------AGTTTTGGACA----TACAA-------------ATGCGAGAATGACCGATGTATGC

Size

Hit Count

Total Norm

Total

M022
Male-body

M040
Female-body

M059
Embryo

M062
Head

V043
Embryo

V051
Head

V112
Male-body

SRR902010
Ovary

SRR902011
Testis

SRR902012
CNS imaginal disc

No Reads

droPer2

TTA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAGACCCGTGGAGA--------------------AGTTTTGGACA----TACAA-------------ATGCGAGAATGACCGATGTATGC	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
No Reads

droAna3

GTTGTTTTTCAAGTTAGAAG--CATGGGAG-----------------------------TTTCAATGGA-----------------TTCCTCTTTTGGCA---------------AAATAAT-TCGATA----------------------------------------TGCG----ATATTATTCGA---------------------------------------TATT--CGATAATATAATATGCGTGGCGCCACCT-AGCGGACTGCGAC-T--CAACTGCAAGGGTATATCAACTTCGGCTCCGCCCGAAGTTAGCTTTCCTTTC--TTGTTAA--TTG-------T-------AA-----------TATT--GT-TATTTA-----TGTA----------------------------------------ATATTGTATACTTGTGAATAAATTGAAA--------------------ATTTTTGGAAC----TATAC-------------ATTA---ATCGAACG--TCATTC	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
.................................................................................................................................................................................................................................................................ACCT-AGCGGACTGCGAC-T--CAACTGC...............................................................................................................................................................................................................................................................	25	20	2.20	44	0	44	0	0	0	0
............................................................TTCAATGGA-----------------TTCCTCTTTTGGCA---------------AAA.......................................................................................................................................................................................................................................................................................................................................................................................................................................	26	20	0.75	15	15	0	0	0	0	0
...........................................................................................................................................................................................................................................................................................TGCAAGGGTATATCAACTTCGGCTCCGC......................................................................................................................................................................................................................................	28	20	0.40	8	8	0	0	0	0	0
...........................................................TTTCAATGGA-----------------TTCCTCTTTTGGCA---------------..........................................................................................................................................................................................................................................................................................................................................................................................................................................	24	20	0.25	5	0	5	0	0	0	0
.............................................................................................................................................................................................................................................................................................CAAGGGTATATCAACTTCGGC...........................................................................................................................................................................................................................................	21	20	0.15	3	0	0	0	0	0	3
..............................................................................................................................................................................................................................................................GCCACCT-AGCGGACTGCGAC-T--CAAC..................................................................................................................................................................................................................................................................	25	20	0.10	2	0	0	0	0	0	2
.............................G-----------------------------TTTCAATGGA-----------------TTCCTCTTTTGGC..........................................................................................................................................................................................................................................................................................................................................................................................................................................................	24	20	0.10	2	2	0	0	0	0	0
.................................................................................................................................................................................................................................................................ACCT-AGCGGACTGCGAC-T--CAACTGG...............................................................................................................................................................................................................................................................	25	20	0.10	2	0	2	0	0	0	0
............................................................TTCAATGGA-----------------TTCCTCTTTTGGCA---------------..........................................................................................................................................................................................................................................................................................................................................................................................................................................	23	20	0.10	2	2	0	0	0	0	0
.....................................................................................................................................................................................................................................................................................................TATCAACTTCGGCTCCGCCCGAAGT...............................................................................................................................................................................................................................	25	20	0.05	1	1	0	0	0	0	0
....................................................................................................................................................................................................................................................................................................................CGCCCGAAGTTAGCTTTCCT.....................................................................................................................................................................................................................	20	20	0.05	1	0	0	0	0	0	1
.............................................................................................................................................................................................................................................................................................CAAGGGTATATCAACTTCGGCTCCG.......................................................................................................................................................................................................................................	25	20	0.05	1	0	0	1	0	0	0
...............................................................................................................................................................................................................................................TATAATATGCGTGGCGCCACCT-AG.....................................................................................................................................................................................................................................................................................	24	20	0.05	1	1	0	0	0	0	0
............................................................................................................................................................................................................................................................................................................................GTTAGCTTTCCTTTC--TTGTTAA--TTG-------T-------AA-----------........................................................................................................................................................................	28	20	0.05	1	1	0	0	0	0	0
...................................................................................................................................................................................................................................................ATATGCGTGGCGCCACCT-AGCGGA.................................................................................................................................................................................................................................................................................	24	20	0.05	1	0	1	0	0	0	0
.............TTAGAAG--CATGGGAG-----------------------------TTTCAATGGA-----------------T......................................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	20	0.05	1	1	0	0	0	0	0
.........CAAGTTAGAAG--CATGGGAG-----------------------------TTTC..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	20	0.05	1	0	1	0	0	0	0
.............................................................................................................................................................................................................................................................................TGCGAC-T--CAACTGCAAGGGTATATCAAC.................................................................................................................................................................................................................................................	28	20	0.05	1	0	0	1	0	0	0
...................G--CATGGGAG-----------------------------TTTCAATGGA-----------------T......................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	20	0.05	1	1	0	0	0	0	0
.............................................................................................................................................................................................................................................................................TGCGAC-T--CAACTGCAAGGGTA........................................................................................................................................................................................................................................................	21	20	0.05	1	0	1	0	0	0	0
...........................................................................................................................................................................................................................................................................................................................AGTTAGCTTTCCTTTC--TTGTTAA--TT.....................................................................................................................................................................................................	25	20	0.05	1	1	0	0	0	0	0
............................................................................................................................................................................................................................................TAATATAATATGCGTGGCGCCACC.........................................................................................................................................................................................................................................................................................	24	20	0.05	1	0	0	1	0	0	0
...................................................................................................................................................................................................................................................ATATGCGTGGCGCCACCT-AGCGGAC................................................................................................................................................................................................................................................................................	25	20	0.05	1	0	1	0	0	0	0
............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GAAC----TATAC-------------ATTA---ATCGAACG--TCATTT	27	20	0.05	1	0	1	0	0	0	0
.................................................................................................................................................................................................................................................TAATATGCGTGGCGCCACCT-AGCGG..................................................................................................................................................................................................................................................................................	25	20	0.05	1	1	0	0	0	0	0
..................................................................................................................................................................................................................................................AATATGCGTGGCGCCACCT-AGC....................................................................................................................................................................................................................................................................................	22	20	0.05	1	0	0	0	0	0	1
...............................................................................................................................................................................................................................................................................................................................AGCTTTCCTTTC--TTGTTAA--TTG-------T-------AA-----------TA......................................................................................................................................................................	27	20	0.05	1	1	0	0	0	0	0

droBip1

GTATTTTTGTAAGATAGAAG--TTCGGGACG-----------------------------T----------------TTTTTTAGTATTTGTTATTG-TA----ATAAATTGGATTATATAT-TCATAT----------------------------------------TCCCATAAGGATCGGCCAACTATATCCGATGTTTGTC----GA---------------T----------ATATAATCCG------------------------GTTTT--AACTTGCAAGGGTATATAAACTTCAGCTCCGCCCGAAGTTAGCTTTCCTTTC--TTGTTTT--TTT-------T-------AA-----------AC--TTCAATTA------------ACATATTTCATAAATAA-----------------ACTTTA------------------TCTAAAAAGG--------------------TT---A--GAA----TGTAATTTGGAATCATCGGTATCAGAAAGAGCCTTATATAT	Size	Hit Count	Total Norm	Total
No Reads

droKik1

AAAGGTGTGAATG-TATAAC--TGT-AACTGTCAAACTGTAAA-CATAA-TAAGTATAGGTAAAATGTTATGAAACTCTGTTTAGTGTCTGTTTTCGGCA---------------T------------------------------------------------------------------------------------------TATAATTTATGATATAAATA----------------------T----------GA-----------AAACC--AATCTGGAAGGGTATACAAACTTCGGCGT--GCCGAAGTTAGCTT-CCTTTC--TTGTTTT--TTATTAATTTT-------AA-----------GATT--AC-TAATTA------------------------------------------------------------------AACAACAAAAT--------------------AATAAATGAAA----CACAA-------------CTTTGAGATCGTCCGTTCCTTGC	Size	Hit Count	Total Norm	Total
No Reads

droFic1

GTCATAG----------------------------------------------------CTTCGATGTT-----------------GT------TTAGCC---------------------T-TTATGT----------------------------------------ACCTATATATTTTGTTCGA--------------------------------CATA-----TT-----------------------------------------TGA----CGACTGCAAGGGTATATCAGCTTTGGCTT--GCCGAAGCTAGCTT-TCTTTC--TTGTTAA--TAA-------T-------AG-----------TTTT--GA-TAA---TAGAATTTAAC---ACTGTCTAGACACGACACTGTGGCAGA-------------T--------TAAGATCATG-TAT--------------------AAATTTAGTTT----TAAAA-------------AGGTAGAGAAAAGCGTTATA---	Size	Hit Count	Total Norm	Total
No Reads

droEle1

CTATCTTATA--------AT--TG----------AGCTGCAAATCATC-------ATAGTTTCAATGTT-----------------TT------TCAGCATATACTCAAGT--------------------------------------------------------------------------------------------------AAATTATAATTTAAATATT-TTCTAAAGTATTTAATTCAT-----------G-----------CAA----TAGCTGCAAGGGTATATGAACTTCGGCTT--GCCGAAGTTTGCTT-TCTTTC--TTGTTATCGTTT-------T-------AA-----------TAG-TTTGATAA------------ATA------------CTCATCGTTA-------AAAGTTTATTA------------------------------------------------TTGTGAGGTACAAGTGA---------------TTGAGATCAA-TTTGGCAGTT	Size	Hit Count	Total Norm	Total
No Reads

droRho1

GGTGTTTTTAAAGTTAGGAA--GATGGGATT-----------------------------T----------------CTTA---------------------------------------------------------------------------TGAGTATTAAGAATGC------------------------------------------ATTATATGAATAATTTT--GGAGAATATAATTTAC-----------A-----------GAA-T--ATTCTGCAAGGGTATATCAACTTCGGCTCCGCCCGAAGTTAGCTTTCCTTTC--TTGTTGT--TAA-------T-------AA-----------AT--ACGT-TT--------TC---CCCAAAATCTCAAATCT-----------------TGATTC------------------TCTGCAAAAA--------------------AG---T--AAA----CGTAA-------------ATGAGAGAATAGTAACTACA---	Size	Hit Count	Total Norm	Total
No Reads

droBia1

TTTATTTCTTATA-TATTAT--CTTAGGCC----AGCAACAATCCT---------------------------------------------------------------------TAAAAATTTC--------------------------------------------------------------------------------------------------------------------------ACATGGTGTTATTAAAGTTGACTATTTC-TTGTAACTGCAAGGGTATACAAACTTCGGCTT--GCCGAAGCTAACTT-CCTTTC--TTGTTATGGTAA-------T-------CA-----------AT--TCAG-TT--------TTCTA-----ATTTTCTAGTCTCTCCG---------------------------------------------------------------------TTTGGCTT----TGATT-------------ATGAAACAGCGATTGATGGATT-	Size	Hit Count	Total Norm	Total
No Reads

droTak1

ATTTTTTTCAATTATAGAAT--ATTTTGTTGT-------------T---------------------------------------------------------------------TATTAAT-TCATGT----------------------------------------TGCT----ATTTTAGTCAA------------------------------------------------------------------------------------GTT----TTCAAGCAAGGGTATTAAAACTTCGGCTT--GCCGAAGTTAGCTT-CCGTCC--TCGTTTT--TAA-------A-------AA-----------TATT--AC-CT----------------------TC-------------------------------------------C---TCCATTGGGAATTATTATACCCGTTACTCG------------------TA-------------GAGTAAAAGGGTACATTGTATTC	Size	Hit Count	Total Norm	Total
No Reads

droEug1

TTG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAGACCCGTCGAGA--------------------AGTCCTGGACT----TACAA-------------GTGCGAGGATAAGCGATGTATAC	Size	Hit Count	Total Norm	Total
No Reads

dm3

A--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GACCCGTCGAGA--------------------AGTCCTGGACG----TACAA-------------GTGCGAGAATGACCGATGCATGC	Size	Hit Count	Total Norm	Total	GSM154618 Ovary	GSM154620 Ovary	GSM154621 Ovary	GSM154622 Ovary	GSM180328 Head	GSM180329 Mixed-body	GSM180330 Embryo	GSM180331 Embryo	GSM180332 Embryo	GSM180333 Embryo	GSM180334 Larvae	GSM180335 Disc	GSM180336 Pupae	GSM180337 S2	GSM231091 Ovary	GSM239041 Head	GSM239050 Head	GSM239051 S2	GSM239052 S2	GSM246084 Head	GSM266765 S2	GSM280082 Ovary	GSM280083 Ovary	GSM280084 Ovary	GSM280085 Testis	GSM280086 Ovary	GSM280087 S2-NP	GSM280088 S2-NP	GSM312995 Head	GSM313160 Head	GSM313161 Head	GSM313162 Head	GSM313163 Head	GSM313164 Head	GSM313165 Head	GSM343287 Embryo	GSM379050 Ovary	GSM379051 Ovary	GSM379052 Ovary	GSM379053 Ovary	GSM379054 Ovary	GSM379055 Ovary	GSM379056 Ovary	GSM379057 Ovary	GSM379058 Ovary	GSM379059 Ovary	GSM379060 Ovary	GSM379061 Ovary	GSM379062 Ovary	GSM379063 Ovary	GSM379064 Ovary	GSM379065 Ovary	GSM379066 Ovary	GSM379067 Ovary	GSM424739 S2	GSM424740 S2	GSM424741 S2	GSM467729 Female-body	GSM467730 Female-body	GSM467731 Female-body	M002 CS	M003 Male-body	M004 Male-body	M005 ML-DmD32	M006 ML-DmD32	M029 OSC	M030 OSC	M031 OSC	M032 S2-R+	M033 S2-R+	M034 S2-R+	M035 S2-R+	M036 S2-R+	M037 S2-R+	M038 S2-R+	M039 S2-R+	M048 S2-R+	OSS2 OSS	OSS6 OSS	OSS7 OSS	OSS8 OSS	S1 Head	S10 Embryo	S11 Embryo	S12 Embryo	S13 Embryo	S14 Embryo	S15 S2-GMR	S16 Kc	S17 Larvae	S18 Larvae	S19 Pupae	S2 Head	S20 Pupae	S21 Disc	S22 Head	S23 Female-body	S24 Male-body	S25 Larvae	S26 Larvae	S27 Pupae	S28 Pupae	S29 Head	S3 Male-body	S30 S2-GMR	S31 S2	S32 S2	S33 Kc	S34 Kc	S35 Kc	S4 Female-body	S5 Disc	S6 Embryo	S7 Embryo	S8 S2	S9 Embryo	SRR001337 Head	SRR001338 Head	SRR001339 Head	SRR001340 Head	SRR001341 Head	SRR001342 Head	SRR001343 Head	SRR001344 Head	SRR001345 Head	SRR001346 Head	SRR001347 Head	SRR001348 Head	SRR001349 Head	SRR001664 Head	SRR010951 Ovary	SRR010952 Ovary	SRR010953 Ovary	SRR010954 Ovary	SRR010955 Ovary	SRR010956 Ovary	SRR010957 Ovary	SRR010958 Ovary	SRR010959 Ovary	SRR010960 Ovary	SRR014268 Embryo	SRR014269 Embryo	SRR014270 Embryo	SRR014271 Embryo	SRR014272 Embryo	SRR014273 Ovary	SRR014274 Ovary	SRR014275 Ovary	SRR014276 Ovary	SRR014277 Ovary	SRR014278 Ovary	SRR014279 Ovary	SRR014280 Ovary	SRR014281 Ovary	SRR014282 Ovary	SRR023197 S2	SRR023399 S2	SRR023400 S2	SRR023402 S2	SRR023407 S2	SRR029028 S2	SRR029029 S2	SRR029030 S2	SRR029031 S2	SRR029032 S2	SRR029033 S2	SRR029608 Head	SRR029633 Head	SRR030145 gb3	SRR031692 Head	SRR031693 Head	SRR031694 Head	SRR031696 Head	SRR031697 Head	SRR031698 Head	SRR031699 Head	SRR031700 Head	SRR031701 Head	SRR031702 Head	SRR031703 Head	SRR031704 Head	SRR032092 S2	SRR032093 S2	SRR032094 S2	SRR032095 S2-NP	SRR032096 S2-NP	SRR032152 Head	SRR060643 Testis	SRR060644 Ovary	SRR060645 Testis	SRR060646 Ovary	SRR060647 Ovary	SRR060648 Ovary	SRR060649 Ovary	SRR060650 Testis	SRR060651 Ovary	SRR060652 Testis	SRR060653 Ovary	SRR065151 Ovary	SRR065152 Ovary	SRR065153 Ovary	SRR065154 Ovary	SRR065155 Ovary	SRR065800 Ovary	SRR065801 Ovary	SRR065802 Ovary	SRR065803 Ovary	SRR065804 Ovary	SRR065805 Ovary	SRR065806 Ovary	SRR065807 Ovary	SRR097865 S2-NP	SRR097866 S2-NP	SRR097867 S2-NP	SRR298536 Ovary	SRR298537 Ovary	SRR298711 Ovary	SRR298712 Ovary	SRR317110 Ovary	SRR317111 Ovary	SRR317112 Ovary	SRR317113 Ovary	SRR317114 Ovary	SRR317115 Ovary	SRR317116 Ovary	SRR317117 Ovary	SRR341115 S2	SRR341116 S2	SRR341117 S2	SRR341118 S2	SRR351332 Mixed-body	SRR351333 Mixed-body	T004 OSC	T005 CME-L1	V001 Embryo	V002 Embryo	V003 S2	V006 Female-body	V007 Head	V008 S2	V009 CME-L1	V010 ML-DmD20-C5	V011 Sg4	V012 Male-body	V013 Head	V014 DTT	V015 DreRFHV	V018 Kc167	V019 GM2	V020 S2-R+	V021 ML-DmD21	V022 ML-DmD32	V023 Head	V024 Kc167	V025 S2-R+	V026 1182-2H	V027 ML-DmD21	V028 CME-W1-Cl.8+	V029 ML-DmD9	V030 ML-DmD8	V031 GM2	V032 S1	V033 CME-W1-Cl.8+	V034 ML-DmD16-C3	V035 ML-DmD32	V036 ML-DmD20-C5	V037 S2	V038 S2	V063 Ovary	V0632 Ovary	V064 Ovary	V0642 Ovary	V065 Ovary	V0652 Ovary	V066 Ovary	V0662 Ovary	V067 S2-NP	V073 mbn2	V074 S3	V075 ML-DmBG1-C1	V076 ML-DmBG3-C2	V077 Head	V078 Head	V079 Head	V080 Head	V081 Embryo	V082 Embryo	V083 Male-body	V084 Female-body	V085 CME-W2	V086 Female-body	V088 Ovary	V0882 Ovary	V089 Ovary	V0892 Ovary	V090 Male-body	V091 fGS/OSS	V092 Male-body	V093 Male-body	V094 S2-R+	V095 S2-R+	V096 Ovary	V097 Ovary	V098 Ovary	V125 ML-DmD9	V126 CME-L1	V127 G2	V128 S3	V129 ML-DmBG1-C1	V130 ML-DmBG3-C2	V131 ML-DmD16-C3	V132 ML-DmD32	V133 Sg4	V134 ML-DmD8	V135 CME-W2	V136 Male-body	V137 Head	V138 Male-body	V139 Female-body	V140 Female-body	V141 Female-body	V142 Female-body	V144 OSC	V145 S2	V146 S1	V147 1182-2H	V148 mbn2	GSM1528798 follicle cells
.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................AA-------------GTGCGAGAATGACCGATGC....	21	1	8.00	8	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	8	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................................................................................................................................................................................................................................................................ACCCGTCGAGA--------------------AGTCCTGGACG----TA.......................................	24	1	4.00	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................GA--------------------AGTCCTGGACG----TACAA-------------GTGCGAGAA..............	27	1	3.00	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................AGA--------------------AGTCCTGGACG----TACAA-------------GTGCGAG................	26	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................AGTCCTGGACG----TACAA-------------GTGC...................	20	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................................................................................................................................................................................................................................................................ACCCGTCGAGA--------------------AGTCCTGGACG----.........................................	22	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ACAA-------------GTGCGAGAATGACCGATGCATGC	27	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGCGAGAATGACCGATGC....	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................................................................................................................................................................................................................................................................................A--------------------AGTCCTGGACG----TACAA-------------GTGCG..................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GTCCTGGACG----TACAA-------------GTGCG..................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TACAA-------------GTGCGAGAATGACCGATGCATG.	27	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0
........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CCTGGACG----TACAA-------------GTGCGG.................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................CGTCGAGA--------------------AGTCCTGGAC..............................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGGACG----TACAA-------------GTGCGAGAATG............	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................AGA--------------------AGTCCTGGACG----TACAA-------------G......................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................A-------------GTGCGAGAATGACCGATG.....	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................GAGA--------------------AGTCCTGGACG----TACA.....................................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0

droSim2

CTTG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAGACCCGTCGAGA--------------------AGTCCTGGACG----TACAA-------------GTGCGAGAATGACCGATGCATGC	Size	Hit Count	Total Norm	Total	M023 Head	M024 Male-body	M025 Embryo	M053 Female-body	O001 Testis	O002 Head	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary	V044 Embryo	SRR902008 Ovary	SRR902009 Testis	SRR1275487 Male larvae	SRR1275485 Male prepupae	SRR1275483 Male prepupae
CTTG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAGACCCGTCGAGA--------------------AGT.....................................................	21	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................G----TACAA-------------GTGCGAGAATGACC.........	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0
..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGGACG----TACAA-------------GTGCGAGAATGACCGA.......	27	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0

droSec2

CA------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAA----CTACTGCAAGGGCATACAAACTTCGGCTT--GTCGCAGTTAATTT-CCCTTC--CTGTTA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
No Reads

droYak3

CTTG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAGACCCGTCGAGA--------------------AGTCCTGGACG----TACAA-------------GTGCGAGAATGACCGATGCATGC	Size	Hit Count	Total Norm	Total	M026 Head	M043 Female-body	M056 Embryo	V046 Embryo	V052 Head	V058 Head	V120 Male-body	SRR1275488 Male larvae	SRR1275486 Male prepupae	SRR1275484 Male prepupae	GSM1528802 follicle cells
...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................G----TACAA-------------GTGCGAGAATGACCGATGC....	25	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0
......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GTCCTGGACG----TACAA-------------GTGC...................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0

droEre2

CTTG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAGACCCGTCGAGA--------------------AGTCCTGGACA----TACAA-------------GTGCGAGAATGACCGATGCATCC	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGGACA----TACAA-------------GTGCGAGAATGACCGA.......	27	1	1.00	1	1	0	0	0	0	0	0
..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CA----TACAA-------------GTGCGAGAATGACCGATGC....	26	1	1.00	1	0	0	0	0	0	1	0

dwi_2186

scf2_1100000004822:1923817-1923967 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-18.6	-18.1