dwi_1832

##################################################--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- GCAACGGGGGAGCCGGCTTTGGCAACAATGGCGGAGGAGGCTCCATAAAGGTATAAAAATCCTGCACGCATACTAAAACCATCTACATTTTAATTTTGGTGACTTTAGCTTTGTTAGTTTTCGAGAAAATCAGTCTTTTTTCATTTTTGGAGGCGGAAATGGGCGTGCCAAAATTTTTAAATAGTCAATATCTGCGCATCTATTAAGCTAGTTTTTATACTAAATTTAATGTCGGTAGCTGTCATAGTAAT ************************************************...(((((((...((((((...(((((..((((..............))))..)))))..............((((.......))))...(((((((((....)))))))))))))))....)))))))......................************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M045 female body	M020 head	V117 male body
.........................CAATGGCGGAGGGAGGTC................................................................................................................................................................................................................	18	3	20	2.05	41	41	0	0
...........................................................................................................................................TTCGTTTTTGGAGCCGGAA.............................................................................................	19	2	1	1.00	1	1	0	0
......................................................................................................................................................................................................................TTATACTAAATTTAATGTCGGTAGC............	25	0	1	1.00	1	1	0	0
............................................................................................................................................................................................TATCTGCGCATCTATTAAGCTAGTT......................................	25	0	5	0.20	1	1	0	0
...........................................................................................................................................................GAAATGGGCGTGCCAAAATTT...........................................................................	21	0	7	0.14	1	0	0	1
....................................................ATAAAACACCTGCACGCCT....................................................................................................................................................................................	19	3	20	0.10	2	2	0	0
...........................................................................................................................................................................................................................CTAAATTTAATGTCGGTAGCT...........	21	0	19	0.05	1	0	1	0
.........................CATTGGCTGAGCAGGCTCC...............................................................................................................................................................................................................	19	3	19	0.05	1	1	0	0
.........................................................................................................................................................................................................................TACTAAATTTAATGTCGGGAGC............	22	1	19	0.05	1	0	1	0
........................................................................................................................................................................................................................ATACTAAATTTAATGTCGGG...............	20	1	20	0.05	1	0	1	0

CGTTGCCCCCTCGGCCGAAACCGTTGTTACCGCCTCCTCCGAGGTATTTCCATATTTTTAGGACGTGCGTATGATTTTGGTAGATGTAAAATTAAAACCACTGAAATCGAAACAATCAAAAGCTCTTTTAGTCAGAAAAAAGTAAAAACCTCCGCCTTTACCCGCACGGTTTTAAAAATTTATCAGTTATAGACGCGTAGATAATTCGATCAAAAATATGATTTAAATTACAGCCATCGACAGTATCATTA ************************************************...(((((((...((((((...(((((..((((..............))))..)))))..............((((.......))))...(((((((((....)))))))))))))))....)))))))......................************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V118 embryo	M045 female body	V117 male body
...........................................................AGGACCTGCTTATGATTT..............................................................................................................................................................................	18	2	5	0.40	2	2	0	0
.........................................................................................................................................................................................................................ATGATTTAAATTACAGCCATCGACAGT.......	27	0	4	0.25	1	1	0	0
..........................................................................TTTCGGTAGATGTAAAATAAAAG..........................................................................................................................................................	23	3	5	0.20	1	0	1	0
...................................................ATAATTTTAGGACGTGGG......................................................................................................................................................................................	18	2	7	0.14	1	0	1	0
...................................................................................................................................................................................TTATCAGTTATAGACGCGTAGATAATT.............................................	27	0	7	0.14	1	1	0	0
....................................................................................................................................................................................TATCAGTTATAGACGCGTAGATAAT..............................................	25	0	9	0.11	1	1	0	0
...........................................................ACGACGTCGGTATGATTTTG............................................................................................................................................................................	20	3	20	0.10	2	0	1	1
.................................................................................................................................................................................................CGCGTAGAAATTTGGATCAA......................................	20	3	15	0.07	1	0	0	1
.......CCCTCGGTGGAACCCGTTG.................................................................................................................................................................................................................................	19	3	20	0.05	1	0	1	0
............................................................................TCGGTAGATGTAAAATAA.............................................................................................................................................................	18	2	20	0.05	1	0	1	0
.................................................................................................CCACTGAAATCGAAACAATC......................................................................................................................................	20	0	20	0.05	1	0	0	1
...................................................................................................................................................................GCACGGTTTTAAAATTTTATCAGTT...............................................................	25	1	20	0.05	1	0	1	0

Species	Coordinate	ID	Alignment
droWil2	scf2_1100000004590:3177348-3177598 -	dwi_1832	GCAACGGGGGAGC------------------------------------CGGCT--TTGGCAACAATG------GCG------GAGGAGGCTCCATAAAGGTATAAAAATC-CTGCACGCATACTAAAACCATCTACATTTTAATTTTGGTGACTTTAGCTTT----------------GTTAGTTTT------------------CGAGAAAATC---------------------------------------------------------AGTCTTTTTTCATTTTTGGAGGCGGAAATGGGCGTGCCAAAATTTTTAAATAGTCAATATCTGCGCATCTATTAAGCTAGTTTTTATACTAAATTTAATGTCGGTAGC---------------TGTCATAGTAA-----T
droVir3	scaffold_12882:2346-2555 +		GAT---ATAAATATA-------------------------------------------------------------------------------------------------CTA---TTATATTGAA------GACATGTCAATTTTGGTGGATCTAGCTCTTACTAT-TCAGC------AAATTTACTCAAAACAACAGGATTTCG---ATATCGATTTTTATCGATTG--------------------------------------------------------CTTGAAGACGGAGAAAGTTATTGT-TTA-TCGGAAAAAAACTCGATCTGCACATGTACTAGGAGGATC------TAAAACTTCACGTCTCTAGC---------------TCTTATAGTTA-----C
droMoj3	scaffold_1668:358-499 -		TCG---TTAGTAC------------------------------------------------------------------------------------------------------------------------------------------------------------------------------T------------------TG---AAATGCGTTTTTTTCAAATT--------------------------------------------------------GGGGCGAACGGAGCGTGGTATCCT-AAATCTGTAAATT-ACAAAATCTTTGTATCCATTAACGTTATATACAACGCAAATTTGAAGTCTCCAGG---------------TCTAAAAGTGTTCAAAT
droGri2	scaffold_14624:442193-442359 +		ATC-----------------------------------------------------------------------------------------------------------------------------------TACATTCCAAGTTTGGTAACTGTAGCTCTTAA------------------ATT---------------------------------------------------------------------------GCGGAAGATATAGGATTTTATCGTTTTTAGGGGCGGGAGTGGGCGTGCTAAAAATTTGTTT-TCC-CGAATTTTGGAATGCAATATAGTAATATACCTTTCAAATTTGGTGTCTCTAGC---------------TTTCAAGTTGT-----G
dp5	4_group3:3080250-3080502 -		GGG---CGCCCCAG--------------------------------------TG--TTGGCATCACTGGAAGATGCCTCCAAAAG-GACGCC----------TCAGGAACTGCTG-GCCTATGCTAAA------AACACCTCGATTCTCGGGGACTT--------------------------------------------------------------------------TTTAAAACCACATTAACACTCCCGCG-----ACGGTTCATACACATTTTCGTCCTTTGCGGGGGCGGAAGGGGGTGCGACGAAATTTTGACACAAAACGGTCAAGGTCCGATATCACAGGAGTGTGGATACCAAATTTGGTTGCTCTGGC---------------TTTTATAGGTT-----C
droPer2	scaffold_36:763384-763618 -		ATG---GGGGGTGGG------------------------------------GCGAAAT------TCTGGGATATACGTCTTATAGTGA-------------------------------CATCTTAAAGGAGTGTGTGTACCAAATTTGGTTACTCTAGCCTTAATAGT-CTCTG------AGATTT---------------------------------------------------------------------------GTGGTTGCCTCAGATTTTCGTCCTTTGCGGGGGCGGAAGGGGGTGTGGCGAAATTTGGACATGAAACGGTCAAGATCCGATATCACAGGAGTGTGGATACCAAATTTGGTTGCTCTGGC---------------TTTTATAGGTT-----C
droAna3	scaffold_1121:1887-1996 -		CAC-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTTGGGGGCGGTAGTGGGCGTGGCACAAATTTGAAACAAACGGGGCCTGCGTTTTATATGCAGCAGTCATCGTGCCAAAATTTAGAGCAGTGTC---------------TCTTATAGTCT-----T
droBip1	scf7180000396710:245632-245848 +		AAA---AGTCTATAAATCCT----------------------------------------------------------------------------------ATATAAATTATCC-AATTTTGC----------TGCATTTTGATTTGTGGG--TGTAGATTAGCC----------------A----------------------------------------------------------TTGTAACAGTTTGGCATCAAAACGCTACATTGAAA----TTTCTGTTTTGGGGGCGGGAGTGGGTGTGCCAACATTTTGACACAAACTTGATCTGCGTGGATACCATACGAGTCCAACTTCCAAATCGGATAGTTTTATC---------------TATTATAGTCT-----T
droKik1	scf7180000302471:1154938-1155040 -		AAG---CTCTGTGAG------------------------------------GCA------------------------------------------------ATAAAAATT-CAAATCGCAAATTCGAATCTTCTACATTCCCAATTCCGTAGCTCGAGGCCC-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ATGCTCCA---------GTCCAGTC---------------TTTTGTCGCTT-----T
droFic1	scf7180000453612:4823-4887 +		CTG---CG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGCTTATAACAAGTATCTACATTTTGAATCCCCGTGTTCTAGC---------------TCTTTTAGTTCATGAGA
droEle1	scf7180000488932:664-742 -		CTA---AAACGTT------------------------------------------------------------------------------------------------------------------------TCATATTTTAATTTTTATAGTTTTGTTTTT----------------GTTTGTTGT------------------------------------------------------------------------------------------------------------------AAGCAAAGCAAAA----------------------------------------------------------------TC---------------TTTTTTTTCTT--TCAG
droRho1	scf7180000766752:30514-30656 +		CAA---AAACAAAAAATAGTCTTGCATTTATAAGCTTCAGCTGA-------------------------------------------------------------AATATT-TAATTCATATTTTGGTAACCTCTTAAATGT---------------------------------------TAGTTTTAAAGCT----------------AAAAGT---------------------------------------------------------ACTTCCT--CTTAATTTGGATACGGAAATGGGAATCT-GAAATTTT------------------------------------------------------------------------------------------AAAA
droBia1	scf7180000301913:10728-10840 +		TTT-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GGCCATTTGTGGGCGTTAAAGTGGGCGTGGCAAACTTTTTTTTAAGTCAATCGATAGGTGTTGATAAACCTAATATATTTCAATAAACA-------------AAAA----------TGTTCTATCATTCAAAC
droTak1	scf7180000415671:168942-169096 +		ATA---TTCAAAGTA------------------------------------TCG------------------------------------------------AAAAATAG----------------------------TTTTAGTTTAGGTGTTAATAATTTT----------------GTCTTTTCC------------------CGAGAAAAAC-ATCTT---------TTTAAGTTTGCATTAAAACT----------------------GACATCTTAATATCTAT-------------------------------AACAAT-----------------------AAGTATAAGTGCTATTACTAAAAATTGT------------------TATTATTAAAT-----T
droEug1	scf7180000408477:1013-1246 -		ACC---GAGATAC------------------------------------CAGCT--TT------TCTAGAAAATGCG------AAAAC-------------------------------CGTTTTGAAGGGATAATAGTACCACTACAAAATGCGCTGACATATAAAGATTACAGATAGATGAGATTT------------------CAACAATTTC---------------------------------------------------------ATTTTTTCGCGATTTATGGGCGTTAGAGTGGGCGTGGCAGGATGCTGTAATAAACTTACGCTGCGTAGGGACTTCACGAATCTTTATCCTTAATCTGAACTTTGTATC---------------TCTTATATTTC-----C
dm3	chr2L:17188118-17188163 -		AAC---TGA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTTTAAATCTTATTTAATATCGATGCC---------------ATTAATAGCAA-----T
droSim2	x:20815601-20815714 -		CAG---T-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTGTGCTGCTTGTGGGCGTTAGATTGGTCGTGGCAACATTTTTAAACAGACATGCGCTGCGTCTATATCTCTGGAAGCTACATGCTTAATCTCA----ACTAGC---------------CTTTATAG--------T
droSec2	scaffold_43:126336-126424 -		TGG---G----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CGTGGCAAAAAGTTTTTT-GGCAAATCGATAGTAATGTATAAGACTAAGAAAACTATGA-----------------AAAAATATCGAAAAGTTTTTTAACAA-----T
droYak3	v2_chrUn_5054:731-924 +		GCA---CACTAAAA--CCATGATGGAT*---ACGCTATAGTCGAGTTCCCCGAC--T----------------------------------------------------------------ATCTGATACCCGTTACTCAGC---------------------------------------TAGTGTAAGTGCG----------------AAGGAC---------------------------------------------------------AGTTTTTGGCGGTTTGTGGGCGTTAGAGTGGGCGTGGACAAAAGTTTTTT-GGCAAATAGATAGAAATTTACAAGACTAATACAAAAATGA-----------------AAAAA----------TATCAAAACAT-----T
droEre2	scaffold_4929:21623482-21623608 +		TTT---TCAAAAGTG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGAC---------------------------------------------------------AGTTTTTGGCGGTTTGTGGGCGTTGAAGGTGGCGTGGCAAAAAGTTTTTT-AGCCAATCTATAG-ATTTTACAAGAT--ATATATTATT------TAAAAGTGG----TAAAA----------TATCACATTTT-----T

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droWil2

GCAACGGGGGAGC------------------------------------CGGCT--TTGGCAACAATG------GCG------GAGGAGGCTCCATAAAGGTATAAAAATC-CTGCACGCATACTAAAACCATCTACATTTTAATTTTGGTGACTTTAGCTTT----------------GTTAGTTTT------------------CGAGAAAATC---------------------------------------------------------AGTCTTTTTTCATTTTTGGAGGCGGAAATGGGCGTGCCAAAATTTTTAAATAGTCAATATCTGCGCATCTATTAAGCTAGTTTTTATACTAAATTTAATGTCGGTAGC---------------TGTCATAGTAA-----T	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
....................................................................................................................................................................................................................................................................................................................................................................TTATACTAAATTTAATGTCGGTAGC---------------.................	25	1	1.00	1	0	1	0	0	0
..........................................................................................................................................................................................................................................................................................................................................TATCTGCGCATCTATTAAGCTAGTT..........................................................	25	5	0.20	1	0	1	0	0	0
.........................................................................................................................................................................................................................................................................................................GAAATGGGCGTGCCAAAATTT...............................................................................................	21	7	0.14	1	0	0	1	0	0
.........................................................................................................................................................................................................................................................................................................................................................................CTAAATTTAATGTCGGTAGC---------------T................	21	19	0.05	1	1	0	0	0	0
......................................................................................................................................................................................................................................................................................................................................................................ATACTAAATTTAATGTCGGG...................................	20	20	0.05	1	1	0	0	0	0

droVir3

GAT---ATAAATATA-------------------------------------------------------------------------------------------------CTA---TTATATTGAA------GACATGTCAATTTTGGTGGATCTAGCTCTTACTAT-TCAGC------AAATTTACTCAAAACAACAGGATTTCG---ATATCGATTTTTATCGATTG--------------------------------------------------------CTTGAAGACGGAGAAAGTTATTGT-TTA-TCGGAAAAAAACTCGATCTGCACATGTACTAGGAGGATC------TAAAACTTCACGTCTCTAGC---------------TCTTATAGTTA-----C	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
..........................................................................................................................................................................................................................................................................................................AGAAAGTTATTGT-TTA-TCGGAA...........................................................................................	22	9	0.11	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................GATTG--------------------------------------------------------CTTGAAGACGGAGAAAGTTAT.........................................................................................................	26	9	0.11	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................................GCACATGTACTAGGAGGATC------T...................................................	21	9	0.11	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................................................................................................................................................................................TCACGTCTCTAGC---------------TCTTATAG.........	21	18	0.06	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droMoj3

TCG---TTAGTAC------------------------------------------------------------------------------------------------------------------------------------------------------------------------------T------------------TG---AAATGCGTTTTTTTCAAATT--------------------------------------------------------GGGGCGAACGGAGCGTGGTATCCT-AAATCTGTAAATT-ACAAAATCTTTGTATCCATTAACGTTATATACAACGCAAATTTGAAGTCTCCAGG---------------TCTAAAAGTGTTCAAAT	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
.......TAGTAC------------------------------------------------------------------------------------------------------------------------------------------------------------------------------T------------------TG---AAATGCGTTTTTTTCAA.........................................................................................................................................................................................	26	9	0.11	1	1	0	0	0	0	0
.........................................................................................................................................................................................................................................................................................................................................................................GCAAATTTGAAGTCTCCAGG---------------TCTA.............	24	20	0.05	1	1	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................................................TATATACAACGCAAATTTGAAGTCTCC...................................	27	20	0.05	1	1	0	0	0	0	0

droGri2

ATC-----------------------------------------------------------------------------------------------------------------------------------TACATTCCAAGTTTGGTAACTGTAGCTCTTAA------------------ATT---------------------------------------------------------------------------GCGGAAGATATAGGATTTTATCGTTTTTAGGGGCGGGAGTGGGCGTGCTAAAAATTTGTTT-TCC-CGAATTTTGGAATGCAATATAGTAATATACCTTTCAAATTTGGTGTCTCTAGC---------------TTTCAAGTTGT-----G	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

dp5

GGG---CGCCCCAG--------------------------------------TG--TTGGCATCACTGGAAGATGCCTCCAAAAG-GACGCC----------TCAGGAACTGCTG-GCCTATGCTAAA------AACACCTCGATTCTCGGGGACTT--------------------------------------------------------------------------TTTAAAACCACATTAACACTCCCGCG-----ACGGTTCATACACATTTTCGTCCTTTGCGGGGGCGGAAGGGGGTGCGACGAAATTTTGACACAAAACGGTCAAGGTCCGATATCACAGGAGTGTGGATACCAAATTTGGTTGCTCTGGC---------------TTTTATAGGTT-----C	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
................................................................................................................................................................................................................................................ACATTAACACTCCCGCG-----ACGGTTC................................................................................................................................................	24	20	2.85	57	0	56	1	0	0	0	0	0	0	0
....................................................................................................................GCCTATGCTAAA------AACACCTCGAT............................................................................................................................................................................................................................................................................	23	20	2.85	57	0	57	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................................................................................TGCGACGAAATTTTGACACAAAACGG..................................................................................	26	2	2.50	5	0	0	0	0	0	0	0	5	0	0
................................................................................................................................................................................................................................................ACATTAACACTCCCGCG-----ACG....................................................................................................................................................	20	20	1.90	38	0	38	0	0	0	0	0	0	0	0
...............................................................CACTGGAAGATGCCTCCAAAAG-GAC....................................................................................................................................................................................................................................................................................................................................	25	20	1.20	24	0	24	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................................................ACACATTTTCGTCCTTTGCGGGGGC.....................................................................................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................ACGCC----------TCAGGAACTGCTG-.........................................................................................................................................................................................................................................................................................................	18	20	0.60	12	0	12	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................................................................................................................................................ATCACAGGAGTGTGGATAC...................................................	19	20	0.45	9	0	9	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................................................................................TCGTCCTTTGCGGGGGCGGAAGG...............................................................................................................	23	20	0.45	9	0	9	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................................................................CATTTTCGTCCTTTGCGGGG.......................................................................................................................	20	5	0.40	2	0	2	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................................................................CATTTTCGTCCTTTGCGGG........................................................................................................................	19	5	0.40	2	0	1	0	0	0	1	0	0	0	0
...................................................................................................................................................TCGGGGACTT--------------------------------------------------------------------------TTTAAAACC.............................................................................................................................................................................	19	16	0.38	6	1	1	0	0	0	0	0	4	0	0
..............................................................................................................................................................................................................................................................................................................................................................GAGTGTGGATACCAAATTTGGTTG.......................................	24	20	0.35	7	0	7	0	0	0	0	0	0	0	0
.....................................................................................................................CCTATGCTAAA------AACACCTCGATTC..........................................................................................................................................................................................................................................................................	24	20	0.35	7	0	7	0	0	0	0	0	0	0	0
......................................................................................................................CTATGCTAAA------AACACCTCGATTC..........................................................................................................................................................................................................................................................................	23	20	0.35	7	0	7	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................................................................TTGGTTGCTCTGGC---------------TTTTATAGG........	23	20	0.35	7	0	3	0	0	0	0	0	4	0	0
................................................................................................................................................................................................................................................ACATTAACACTCCCGCG-----ACGGT..................................................................................................................................................	22	20	0.35	7	0	0	0	0	1	0	0	5	1	0
................................................................................................................................................................................................................................................ACATTAACACTCCCGCG-----ACGGTTCATAC............................................................................................................................................	28	20	0.30	6	0	5	0	0	0	0	0	0	1	0
.................................................................................................................................................................................................................................................CATTAACACTCCCGCG-----ACGGTTC................................................................................................................................................	23	20	0.30	6	0	6	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................................................ACATTAACACTCCCGCG-----ACGG...................................................................................................................................................	21	20	0.30	6	0	0	0	0	0	0	3	0	3	0
.................................................................................................................................................................................................................................................CATTAACACTCCCGCG-----ACGGTT.................................................................................................................................................	22	20	0.25	5	0	5	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................................................................CATTTTCGTCCTTTGCGGGGG......................................................................................................................	21	4	0.25	1	0	1	0	0	0	0	0	0	0	0
.................................................................CTGGAAGATGCCTCCAAAAG-G......................................................................................................................................................................................................................................................................................................................................	21	20	0.25	5	0	5	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................................................................................................................CAAGGTCCGATATCACAGGAGTGTGGA......................................................	27	20	0.25	5	0	5	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................................................................................................................................TGTGGATACCAAATTTGGTT........................................	20	20	0.25	5	0	5	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................................................................................................................................................................................TGGTTGCTCTGGC---------------TTTTATAGG........	22	20	0.20	4	0	0	0	0	0	0	0	4	0	0
......................................................................................................................................................................................................................................................................................................................................................TATCACAGGAGTGTGGATACCAAATC.............................................	26	20	0.20	4	0	0	4	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................................................................................................................TCCGATATCACAGGAGTGTG........................................................	20	20	0.20	4	0	4	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................................................ACATTAACACTCCCGCG-----ACGGTTCAT..............................................................................................................................................	26	20	0.20	4	0	3	0	0	0	0	0	0	1	0
.......................................................................................................................TATGCTAAA------AACACCTCGATTC..........................................................................................................................................................................................................................................................................	22	20	0.20	4	0	4	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................................................................................................................TGACACAAAACGGTCAAGGTCCGA.......................................................................	24	20	0.20	4	0	3	0	0	0	1	0	0	0	0
................................................................................................................................................................................................................................................ACATTAACACTCCCGCG-----AC.....................................................................................................................................................	19	20	0.20	4	0	4	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................................................ACATTAACACTCCCGCG-----ACGGTTA................................................................................................................................................	24	20	0.20	4	0	4	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................CATTAACACTCCCGCG-----ACG....................................................................................................................................................	19	20	0.20	4	0	4	0	0	0	0	0	0	0	0
........................................................................................................................................................................................................................................................................................................................................CGGTCAAGGTCCGATATCACAGGAG............................................................	25	20	0.15	3	0	0	0	0	0	0	0	3	0	0
................................................................ACTGGAAGATGCCTCCAAAA.........................................................................................................................................................................................................................................................................................................................................	20	20	0.15	3	0	3	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................................................ACATTAACACTCCCGCG-----ACGGTTCATA.............................................................................................................................................	27	20	0.15	3	0	3	0	0	0	0	0	0	0	0
...............................................................CACTGGAAGATGCCTCCAAAAG-GACGCC----------T......................................................................................................................................................................................................................................................................................................................	29	20	0.15	3	0	2	0	0	0	0	0	1	0	0
...................................................................................................................................................TCGGGGACTT--------------------------------------------------------------------------TTTAAAAAA.............................................................................................................................................................................	19	20	0.15	3	1	0	0	0	0	0	0	2	0	0
...................................................................................................................................................................................................................................................................................................................................................CGATATCACAGGAGTGTGGATAC...................................................	23	20	0.15	3	0	0	0	0	3	0	0	0	0	0
...................................................................................................................................................................................................................................................................................................................................................CGATATCACAGGAGTGTGGATACCAA................................................	26	20	0.15	3	0	0	0	0	0	0	0	3	0	0
....................................................................................................................................................CGGGGACTT--------------------------------------------------------------------------TTTAAAACC.............................................................................................................................................................................	18	16	0.13	2	1	1	0	0	0	0	0	0	0	0
...............................................................CACTGGAAGATGCCTCCAAAAG-GACA...................................................................................................................................................................................................................................................................................................................................	26	20	0.10	2	0	2	0	0	0	0	0	0	0	0
............................................................CATCACTGGAAGATGCCTCC.............................................................................................................................................................................................................................................................................................................................................	20	20	0.10	2	0	2	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................................................................................................................................................AATTTGGTTGCTCTGGC---------------TTTTATAG.........	25	20	0.10	2	0	0	0	0	0	0	0	2	0	0
................................................................................................................................................................................................................................................ACATTAACACTCCCGCG-----ACGA...................................................................................................................................................	21	20	0.10	2	0	2	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................................................................................................................................................................................TGGTTGCTCTGGC---------------TTTTAT...........	19	20	0.10	2	0	0	0	0	0	0	0	1	1	0
.......................................................................................................................TATGCTAAA------AACACCTCGATTCTCGGG.....................................................................................................................................................................................................................................................................	27	20	0.10	2	0	1	1	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................................................................................................................................................................TTTGGTTGCTCTGGC---------------TTTTATAGGTT-----C	27	20	0.10	2	0	0	0	0	0	0	0	2	0	0
...................................................................................................................................................................................................................................................................................................................................................................................TTGCTCTGGC---------------TTTTATAGGTT-----C	22	20	0.10	2	0	0	0	0	0	0	0	2	0	0
..................................................................................AAG-GACGCC----------TCAGGAACTGCTG-G........................................................................................................................................................................................................................................................................................................	23	20	0.10	2	0	2	0	0	0	0	0	0	0	0
........................................................................................................................................................................................................................................................................................................................................CGGTCAAGGTCCGATATCACAGG..............................................................	23	20	0.10	2	0	2	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................................ATTAACACTCCCGCG-----ACGGTTC................................................................................................................................................	22	20	0.10	2	0	2	0	0	0	0	0	0	0	0
..............................................................................CCAAAAG-GACGCC----------TCAGGAACTGC............................................................................................................................................................................................................................................................................................................	24	20	0.10	2	0	2	0	0	0	0	0	0	0	0
............................................................CATCACTGGAAGATGCCTCCAAA..........................................................................................................................................................................................................................................................................................................................................	23	20	0.10	2	0	2	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................................................................................................................................................................GTTGCTCTGGC---------------TTTTATAGGT.......	21	20	0.10	2	0	2	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................................................................................TTCGTCCTTTGCGGGGGCGGAAG................................................................................................................	23	20	0.10	2	0	0	0	0	0	0	2	0	0	0
.............................................................................................................................................................................................................................................................................................................................................AAGGTCCGATATCACAGGAGT...........................................................	21	20	0.10	2	0	0	0	0	0	2	0	0	0	0
..........................................................................................................................GCTAAA------AACACCTCGATTCTCGGG.....................................................................................................................................................................................................................................................................	24	20	0.10	2	0	2	0	0	0	0	0	0	0	0
.........................................................................TGCCTCCAAAAG-GACGCC----------TCAGG..................................................................................................................................................................................................................................................................................................................	23	20	0.10	2	0	2	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................................ATTAACACTCCCGCG-----ACGGTTCATAC............................................................................................................................................	26	20	0.10	2	0	1	0	0	0	0	0	0	1	0
................................................................................................................................................................................................................................................ACATTAACACTCCCGCG-----ACGGTG.................................................................................................................................................	23	20	0.10	2	0	2	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................................................................TAAAACCACATTAACACTCCCGCG-----ACG....................................................................................................................................................	27	20	0.05	1	0	1	0	0	0	0	0	0	0	0
..............................................................TCACTGGAAGATGCCTCCAAAAG-GACGC..................................................................................................................................................................................................................................................................................................................................	28	20	0.05	1	0	1	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................AACCACATTAACACTCCCGCG-----ACGGT..................................................................................................................................................	26	20	0.05	1	0	1	0	0	0	0	0	0	0	0
................................................................................AAAAG-GACGCC----------TCAGGAACTG.............................................................................................................................................................................................................................................................................................................	21	20	0.05	1	0	0	0	0	0	0	0	0	1	0
...............................................................CACTGGAAGATGCCTCCAAAAG-GACGA..................................................................................................................................................................................................................................................................................................................................	27	20	0.05	1	0	1	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................AACCACATTAACACTCCCGCG-----ACGGTT.................................................................................................................................................	27	20	0.05	1	0	1	0	0	0	0	0	0	0	0
.........................................................................................................................TGCTAAA------AACACCTCGATTCTCGG......................................................................................................................................................................................................................................................................	24	20	0.05	1	0	1	0	0	0	0	0	0	0	0
...................................................................GGAAGATGCCTCCAAAAG-G......................................................................................................................................................................................................................................................................................................................................	19	20	0.05	1	0	1	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................................................................................................................................................................................TGGTTGCTCTGGC---------------TTTTATAGGT.......	23	20	0.05	1	0	1	0	0	0	0	0	0	0	0
.........................................................................TGCCTCCAAAAG-GACGCC----------TCAG...................................................................................................................................................................................................................................................................................................................	22	20	0.05	1	0	1	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................................................................................................................................................TATCACAGGAGTGTGGATAC...................................................	20	20	0.05	1	0	0	0	0	0	1	0	0	0	0
.............................................................................................................................................................................................................................................................................................................................................................................ATTTGGTTGCTCTGGC---------------TTTT.............	20	20	0.05	1	0	0	0	0	0	0	1	0	0	0
...........CAG--------------------------------------TG--TTGGCATCACTGGAAGATGCC................................................................................................................................................................................................................................................................................................................................................	26	20	0.05	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................................GGTCCGATATCACAGGAGTG..........................................................	20	20	0.05	1	0	0	0	0	0	0	1	0	0	0
..........................................................................................................................GCTAAA------AACACCTCGATTCTCGG......................................................................................................................................................................................................................................................................	23	20	0.05	1	0	0	0	0	0	0	0	0	1	0
.................................................................................AAAG-GACGCC----------TCAGGAACTGCTG-G........................................................................................................................................................................................................................................................................................................	24	20	0.05	1	0	0	0	0	0	0	0	0	1	0
.......................................................................................................................TATGCTAAA------AACACCTCGATTCTCG.......................................................................................................................................................................................................................................................................	25	20	0.05	1	0	1	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................................................................................................................................................................TTTGGTTGCTCTGGC---------------TTTTGC...........	21	20	0.05	1	0	1	0	0	0	0	0	0	0	0
.............................................................................TCCAAAAG-GACGCC----------TCAGGAACTGC............................................................................................................................................................................................................................................................................................................	25	20	0.05	1	0	1	0	0	0	0	0	0	0	0
...................................................................GGAAGATGCCTCCAAAAG-GAC....................................................................................................................................................................................................................................................................................................................................	21	20	0.05	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................................................................................................................................................................TACCAAATTTGGTTGCTCTGGC---------------TTTTA............	27	20	0.05	1	0	1	0	0	0	0	0	0	0	0
...............................................................CACTGGAAGATGCCTCCAAAAG-GACT...................................................................................................................................................................................................................................................................................................................................	26	20	0.05	1	0	1	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................................................................................TTTTCGTCCTTTGCGGGGGC.....................................................................................................................	20	20	0.05	1	1	0	0	0	0	0	0	0	0	0
......................................................................................................................CTATGCTAAA------AACACCTCGATT...........................................................................................................................................................................................................................................................................	22	20	0.05	1	0	0	1	0	0	0	0	0	0	0
................................................................................................................................................................................................................................................ACATTAACACTCCCGCG-----ACGGC..................................................................................................................................................	22	20	0.05	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................................................................................................CACAAAACGGTCAAGGTCCGA.......................................................................	21	20	0.05	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................................................................TTGGTTGCTCTGGC---------------TTTTATAGGT.......	24	20	0.05	1	0	1	0	0	0	0	0	0	0	0
..............................................................TCACTGGAAGATGCCTCCAAAAG-GAC....................................................................................................................................................................................................................................................................................................................................	26	20	0.05	1	0	1	0	0	0	0	0	0	0	0
....................................................................................................................GCCTATGCTAAA------AACACCTCGATTCA.........................................................................................................................................................................................................................................................................	26	20	0.05	1	0	1	0	0	0	0	0	0	0	0
..........................................................................................................................................................................................................................................AAAACCACATTAACACTCCCGCG-----.......................................................................................................................................................	23	20	0.05	1	0	1	0	0	0	0	0	0	0	0
..................................................................TGGAAGATGCCTCCAAAAG-GACGCC----------.......................................................................................................................................................................................................................................................................................................................	25	20	0.05	1	0	0	0	0	0	0	0	1	0	0
..............................................................................CCAAAAG-GACGCC----------TCAGGAACTGCTG-.........................................................................................................................................................................................................................................................................................................	26	20	0.05	1	0	0	0	1	0	0	0	0	0	0
.............................................................................................................................................................................................................................................ACCACATTAACACTCCCGCG-----ACG....................................................................................................................................................	23	20	0.05	1	0	1	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................................................ACATTAACACTCCCGCG-----ACGGTC.................................................................................................................................................	23	20	0.05	1	0	0	0	0	0	0	0	0	1	0
................................................................ACTGGAAGATGCCTCCAAAAG-GACGCC----------T......................................................................................................................................................................................................................................................................................................................	28	20	0.05	1	0	1	0	0	0	0	0	0	0	0
..................................................................................................................G-GCCTATGCTAAA------AACACCTC...............................................................................................................................................................................................................................................................................	21	20	0.05	1	0	1	0	0	0	0	0	0	0	0
........................................................................................................................ATGCTAAA------AACACCTCGATTCTCGG......................................................................................................................................................................................................................................................................	25	20	0.05	1	0	1	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................................................ACATTAACACTCCCGCG-----ACGGTTT................................................................................................................................................	24	20	0.05	1	0	1	0	0	0	0	0	0	0	0
........................................................................................................................................................................................................................................................................................................................AAATTTTGACACAAAACGGTC................................................................................	21	20	0.05	1	0	0	0	1	0	0	0	0	0	0
.....................................................................................................................................................................................................................................................................................................................................................ATATCACAGGAGTGTGGATACCA.................................................	23	20	0.05	1	0	0	0	0	0	0	0	0	1	0
..................................................................................................................................................................................................................................................ATTAACACTCCCGCG-----ACG....................................................................................................................................................	18	20	0.05	1	0	1	0	0	0	0	0	0	0	0
........................................................................................................AGGAACTGCTG-GCCTATGCT................................................................................................................................................................................................................................................................................................	20	20	0.05	1	0	1	0	0	0	0	0	0	0	0
.........................................................................TGCCTCCAAAAG-GACGCC----------TCAGGAA................................................................................................................................................................................................................................................................................................................	25	20	0.05	1	0	1	0	0	0	0	0	0	0	0
...............................................................CACTGGAAGATGCCTCCAAAAG-GACGCC----------.......................................................................................................................................................................................................................................................................................................................	28	20	0.05	1	0	0	0	0	0	0	0	1	0	0
.................................................................................AAAG-GACGCC----------TCAGGAACTGC............................................................................................................................................................................................................................................................................................................	21	20	0.05	1	0	0	0	0	0	0	0	0	1	0
..............................................................................................................................................................................................................................................................................................................................................................................TTTGGTTGCTCTGGC---------------TTTTATAGG........	24	20	0.05	1	0	1	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................................................ACATTAACACTCCCGCG-----ACGGTAA................................................................................................................................................	24	20	0.05	1	0	0	1	0	0	0	0	0	0	0
........................................................................................................................ATGCTAAA------AACACCTCGATC...........................................................................................................................................................................................................................................................................	20	20	0.05	1	0	0	1	0	0	0	0	0	0	0
..................................................................................AAG-GACGCC----------TCAGGAACTG.............................................................................................................................................................................................................................................................................................................	19	20	0.05	1	0	0	0	0	0	0	0	0	1	0
..............................................................TCACTGGAAGATGCCTCCAAAAG-GACG...................................................................................................................................................................................................................................................................................................................................	27	20	0.05	1	0	1	0	0	0	0	0	0	0	0
...........................................................................................................................................................................................................................................AAACCACATTAACACTCCCGCG-----A......................................................................................................................................................	23	20	0.05	1	0	1	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................................................................................................................................................TATCACAGGAGTGTGGATACC..................................................	21	20	0.05	1	0	0	0	0	0	0	0	1	0	0
.............................................................ATCACTGGAAGATGCCTCCAAAA.........................................................................................................................................................................................................................................................................................................................................	23	20	0.05	1	0	1	0	0	0	0	0	0	0	0
....................................................................................................................GCCTATGCTAAA------AACACCTCGAA............................................................................................................................................................................................................................................................................	23	20	0.05	1	0	1	0	0	0	0	0	0	0	0
....................................................TG--TTGGCATCACTGGAAGATG..................................................................................................................................................................................................................................................................................................................................................	21	20	0.05	1	0	0	0	0	0	0	1	0	0	0
..........................................................................................................................................................................................................................................................................................................................................................................CAAATTTGGTTGCTCTGGC---------------TT...............	21	20	0.05	1	0	0	0	0	0	0	1	0	0	0
............................................................................................................................................................................................................................................................................................................................................................................AATTTGGTTGCTCTGGC---------------TTTTATAGGT.......	27	20	0.05	1	0	1	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................................................................................................................................................................GTTGCTCTGGC---------------TTTTATG..........	18	20	0.05	1	0	1	0	0	0	0	0	0	0	0
............................................................CATCACTGGAAGATGCCTC..............................................................................................................................................................................................................................................................................................................................................	19	20	0.05	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................................................................................................................................TGTGGATACCAAATTTGGTTGCTCTGG.................................	27	20	0.05	1	0	1	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................................................................TAAAACCACATTAACACTCCCG..............................................................................................................................................................	22	20	0.05	1	0	1	0	0	0	0	0	0	0	0

droPer2

ATG---GGGGGTGGG------------------------------------GCGAAAT------TCTGGGATATACGTCTTATAGTGA-------------------------------CATCTTAAAGGAGTGTGTGTACCAAATTTGGTTACTCTAGCCTTAATAGT-CTCTG------AGATTT---------------------------------------------------------------------------GTGGTTGCCTCAGATTTTCGTCCTTTGCGGGGGCGGAAGGGGGTGTGGCGAAATTTGGACATGAAACGGTCAAGATCCGATATCACAGGAGTGTGGATACCAAATTTGGTTGCTCTGGC---------------TTTTATAGGTT-----C	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
.......................................................................................................................................................................................................................................................................................................................GAAATTTGGACATGAAACGG..................................................................................	20	20	0.30	6	0	0	0	0	0	6
..................................................................................................................................................................................................................................................................................................................................................CCGATATCACAGGAGTGTGGA......................................................	21	20	0.25	5	0	5	0	0	0	0
...................................................................................................................................................................AATAGT-CTCTG------AGATTT---------------------------------------------------------------------------GTGG...................................................................................................................................................	21	20	0.15	3	0	0	0	0	0	3
.............................................................................................................................................................................................................................................................................................................................................AAGATCCGATATCACAGGAGT...........................................................	21	20	0.05	1	0	1	0	0	0	0
............................................................................................................................................................................................................................................................................................................................................CAAGATCCGATATCACAGGAGTGTG........................................................	25	20	0.05	1	0	1	0	0	0	0
............................................................................................................................................................................................................................................................................................................................................CAAGATCCGATATCACAGGAGTGTGG.......................................................	26	20	0.05	1	0	1	0	0	0	0
.......................................................................................................................................................................................................................................................................................................................................................................TACCAAATTTGGTTGCTCTGG.................................	21	20	0.05	1	0	0	1	0	0	0

droAna3

CAC-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTTGGGGGCGGTAGTGGGCGTGGCACAAATTTGAAACAAACGGGGCCTGCGTTTTATATGCAGCAGTCATCGTGCCAAAATTTAGAGCAGTGTC---------------TCTTATAGTCT-----T	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
No Reads

droBip1

AAA---AGTCTATAAATCCT----------------------------------------------------------------------------------ATATAAATTATCC-AATTTTGC----------TGCATTTTGATTTGTGGG--TGTAGATTAGCC----------------A----------------------------------------------------------TTGTAACAGTTTGGCATCAAAACGCTACATTGAAA----TTTCTGTTTTGGGGGCGGGAGTGGGTGTGCCAACATTTTGACACAAACTTGATCTGCGTGGATACCATACGAGTCCAACTTCCAAATCGGATAGTTTTATC---------------TATTATAGTCT-----T	Size	Hit Count	Total Norm	Total
No Reads

droKik1

AAG---CTCTGTGAG------------------------------------GCA------------------------------------------------ATAAAAATT-CAAATCGCAAATTCGAATCTTCTACATTCCCAATTCCGTAGCTCGAGGCCC-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ATGCTCCA---------GTCCAGTC---------------TTTTGTCGCTT-----T	Size	Hit Count	Total Norm	Total
No Reads

droFic1

CTG---CG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGCTTATAACAAGTATCTACATTTTGAATCCCCGTGTTCTAGC---------------TCTTTTAGTTCATGAGA	Size	Hit Count	Total Norm	Total
No Reads

droEle1

CTA---AAACGTT------------------------------------------------------------------------------------------------------------------------TCATATTTTAATTTTTATAGTTTTGTTTTT----------------GTTTGTTGT------------------------------------------------------------------------------------------------------------------AAGCAAAGCAAAA----------------------------------------------------------------TC---------------TTTTTTTTCTT--TCAG	Size	Hit Count	Total Norm	Total
No Reads

droRho1

CAA---AAACAAAAAATAGTCTTGCATTTATAAGCTTCAGCTGA-------------------------------------------------------------AATATT-TAATTCATATTTTGGTAACCTCTTAAATGT---------------------------------------TAGTTTTAAAGCT----------------AAAAGT---------------------------------------------------------ACTTCCT--CTTAATTTGGATACGGAAATGGGAATCT-GAAATTTT------------------------------------------------------------------------------------------AAAA	Size	Hit Count	Total Norm	Total
No Reads

droBia1

TTT-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GGCCATTTGTGGGCGTTAAAGTGGGCGTGGCAAACTTTTTTTTAAGTCAATCGATAGGTGTTGATAAACCTAATATATTTCAATAAACA-------------AAAA----------TGTTCTATCATTCAAAC	Size	Hit Count	Total Norm	Total
No Reads

droTak1

ATA---TTCAAAGTA------------------------------------TCG------------------------------------------------AAAAATAG----------------------------TTTTAGTTTAGGTGTTAATAATTTT----------------GTCTTTTCC------------------CGAGAAAAAC-ATCTT---------TTTAAGTTTGCATTAAAACT----------------------GACATCTTAATATCTAT-------------------------------AACAAT-----------------------AAGTATAAGTGCTATTACTAAAAATTGT------------------TATTATTAAAT-----T	Size	Hit Count	Total Norm	Total
No Reads

droEug1

ACC---GAGATAC------------------------------------CAGCT--TT------TCTAGAAAATGCG------AAAAC-------------------------------CGTTTTGAAGGGATAATAGTACCACTACAAAATGCGCTGACATATAAAGATTACAGATAGATGAGATTT------------------CAACAATTTC---------------------------------------------------------ATTTTTTCGCGATTTATGGGCGTTAGAGTGGGCGTGGCAGGATGCTGTAATAAACTTACGCTGCGTAGGGACTTCACGAATCTTTATCCTTAATCTGAACTTTGTATC---------------TCTTATATTTC-----C	Size	Hit Count	Total Norm	Total
No Reads

dm3

AAC---TGA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTTTAAATCTTATTTAATATCGATGCC---------------ATTAATAGCAA-----T	Size	Hit Count	Total Norm	Total	GSM154618 Ovary	GSM154620 Ovary	GSM154621 Ovary	GSM154622 Ovary	GSM180328 Head	GSM180329 Mixed-body	GSM180330 Embryo	GSM180331 Embryo	GSM180332 Embryo	GSM180333 Embryo	GSM180334 Larvae	GSM180335 Disc	GSM180336 Pupae	GSM180337 S2	GSM231091 Ovary	GSM239041 Head	GSM239050 Head	GSM239051 S2	GSM239052 S2	GSM246084 Head	GSM266765 S2	GSM280082 Ovary	GSM280083 Ovary	GSM280084 Ovary	GSM280085 Testis	GSM280086 Ovary	GSM280087 S2-NP	GSM280088 S2-NP	GSM312995 Head	GSM313160 Head	GSM313161 Head	GSM313162 Head	GSM313163 Head	GSM313164 Head	GSM313165 Head	GSM343287 Embryo	GSM379050 Ovary	GSM379051 Ovary	GSM379052 Ovary	GSM379053 Ovary	GSM379054 Ovary	GSM379055 Ovary	GSM379056 Ovary	GSM379057 Ovary	GSM379058 Ovary	GSM379059 Ovary	GSM379060 Ovary	GSM379061 Ovary	GSM379062 Ovary	GSM379063 Ovary	GSM379064 Ovary	GSM379065 Ovary	GSM379066 Ovary	GSM379067 Ovary	GSM424739 S2	GSM424740 S2	GSM424741 S2	GSM467729 Female-body	GSM467730 Female-body	GSM467731 Female-body	M002 CS	M003 Male-body	M004 Male-body	M005 ML-DmD32	M006 ML-DmD32	M029 OSC	M030 OSC	M031 OSC	M032 S2-R+	M033 S2-R+	M034 S2-R+	M035 S2-R+	M036 S2-R+	M037 S2-R+	M038 S2-R+	M039 S2-R+	M048 S2-R+	OSS2 OSS	OSS6 OSS	OSS7 OSS	OSS8 OSS	S1 Head	S10 Embryo	S11 Embryo	S12 Embryo	S13 Embryo	S14 Embryo	S15 S2-GMR	S16 Kc	S17 Larvae	S18 Larvae	S19 Pupae	S2 Head	S20 Pupae	S21 Disc	S22 Head	S23 Female-body	S24 Male-body	S25 Larvae	S26 Larvae	S27 Pupae	S28 Pupae	S29 Head	S3 Male-body	S30 S2-GMR	S31 S2	S32 S2	S33 Kc	S34 Kc	S35 Kc	S4 Female-body	S5 Disc	S6 Embryo	S7 Embryo	S8 S2	S9 Embryo	SRR001337 Head	SRR001338 Head	SRR001339 Head	SRR001340 Head	SRR001341 Head	SRR001342 Head	SRR001343 Head	SRR001344 Head	SRR001345 Head	SRR001346 Head	SRR001347 Head	SRR001348 Head	SRR001349 Head	SRR001664 Head	SRR010951 Ovary	SRR010952 Ovary	SRR010953 Ovary	SRR010954 Ovary	SRR010955 Ovary	SRR010956 Ovary	SRR010957 Ovary	SRR010958 Ovary	SRR010959 Ovary	SRR010960 Ovary	SRR014268 Embryo	SRR014269 Embryo	SRR014270 Embryo	SRR014271 Embryo	SRR014272 Embryo	SRR014273 Ovary	SRR014274 Ovary	SRR014275 Ovary	SRR014276 Ovary	SRR014277 Ovary	SRR014278 Ovary	SRR014279 Ovary	SRR014280 Ovary	SRR014281 Ovary	SRR014282 Ovary	SRR023197 S2	SRR023399 S2	SRR023400 S2	SRR023402 S2	SRR023407 S2	SRR029028 S2	SRR029029 S2	SRR029030 S2	SRR029031 S2	SRR029032 S2	SRR029033 S2	SRR029608 Head	SRR029633 Head	SRR030145 gb3	SRR031692 Head	SRR031693 Head	SRR031694 Head	SRR031696 Head	SRR031697 Head	SRR031698 Head	SRR031699 Head	SRR031700 Head	SRR031701 Head	SRR031702 Head	SRR031703 Head	SRR031704 Head	SRR032092 S2	SRR032093 S2	SRR032094 S2	SRR032095 S2-NP	SRR032096 S2-NP	SRR032152 Head	SRR060643 Testis	SRR060644 Ovary	SRR060645 Testis	SRR060646 Ovary	SRR060647 Ovary	SRR060648 Ovary	SRR060649 Ovary	SRR060650 Testis	SRR060651 Ovary	SRR060652 Testis	SRR060653 Ovary	SRR065151 Ovary	SRR065152 Ovary	SRR065153 Ovary	SRR065154 Ovary	SRR065155 Ovary	SRR065800 Ovary	SRR065801 Ovary	SRR065802 Ovary	SRR065803 Ovary	SRR065804 Ovary	SRR065805 Ovary	SRR065806 Ovary	SRR065807 Ovary	SRR097865 S2-NP	SRR097866 S2-NP	SRR097867 S2-NP	SRR298536 Ovary	SRR298537 Ovary	SRR298711 Ovary	SRR298712 Ovary	SRR317110 Ovary	SRR317111 Ovary	SRR317112 Ovary	SRR317113 Ovary	SRR317114 Ovary	SRR317115 Ovary	SRR317116 Ovary	SRR317117 Ovary	SRR341115 S2	SRR341116 S2	SRR341117 S2	SRR341118 S2	SRR351332 Mixed-body	SRR351333 Mixed-body	T004 OSC	T005 CME-L1	V001 Embryo	V002 Embryo	V003 S2	V006 Female-body	V007 Head	V008 S2	V009 CME-L1	V010 ML-DmD20-C5	V011 Sg4	V012 Male-body	V013 Head	V014 DTT	V015 DreRFHV	V018 Kc167	V019 GM2	V020 S2-R+	V021 ML-DmD21	V022 ML-DmD32	V023 Head	V024 Kc167	V025 S2-R+	V026 1182-2H	V027 ML-DmD21	V028 CME-W1-Cl.8+	V029 ML-DmD9	V030 ML-DmD8	V031 GM2	V032 S1	V033 CME-W1-Cl.8+	V034 ML-DmD16-C3	V035 ML-DmD32	V036 ML-DmD20-C5	V037 S2	V038 S2	V063 Ovary	V0632 Ovary	V064 Ovary	V0642 Ovary	V065 Ovary	V0652 Ovary	V066 Ovary	V0662 Ovary	V067 S2-NP	V073 mbn2	V074 S3	V075 ML-DmBG1-C1	V076 ML-DmBG3-C2	V077 Head	V078 Head	V079 Head	V080 Head	V081 Embryo	V082 Embryo	V083 Male-body	V084 Female-body	V085 CME-W2	V086 Female-body	V088 Ovary	V0882 Ovary	V089 Ovary	V0892 Ovary	V090 Male-body	V091 fGS/OSS	V092 Male-body	V093 Male-body	V094 S2-R+	V095 S2-R+	V096 Ovary	V097 Ovary	V098 Ovary	V125 ML-DmD9	V126 CME-L1	V127 G2	V128 S3	V129 ML-DmBG1-C1	V130 ML-DmBG3-C2	V131 ML-DmD16-C3	V132 ML-DmD32	V133 Sg4	V134 ML-DmD8	V135 CME-W2	V136 Male-body	V137 Head	V138 Male-body	V139 Female-body	V140 Female-body	V141 Female-body	V142 Female-body	V144 OSC	V145 S2	V146 S1	V147 1182-2H	V148 mbn2	GSM1528798 follicle cells
.....................................................................................................................................................................................................................................................................................................................................................................................TCGATGCC---------------ATTAATAGGC.......	18	4	0.25	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droSim2

CAG---T-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTGTGCTGCTTGTGGGCGTTAGATTGGTCGTGGCAACATTTTTAAACAGACATGCGCTGCGTCTATATCTCTGGAAGCTACATGCTTAATCTCA----ACTAGC---------------CTTTATAG--------T	Size	Hit Count	Total Norm	Total	M023 Head	M024 Male-body	M025 Embryo	M053 Female-body	O001 Testis	O002 Head	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary	V044 Embryo	SRR902008 Ovary	SRR902009 Testis	SRR1275487 Male larvae	SRR1275485 Male prepupae	SRR1275483 Male prepupae
.......................................................................................................................................................................................................................................................................................................................................................ATCTCTGGAAGCTACATGCTT.................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
.............................................................................................................................................................................................................................................................................................................................TTTAAACAGACATGCGCTGCGTCTATA.....................................................................	27	1	1.00	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0
..........................................................................................................................................................................................................................................................................................................................................................TCTGGAAGCTACATGCTTAA...............................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
.....................................................................................................................................................................................................................................................................................................CGTTAGATTGGTCGTGGCAACATTT...............................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0
.............................................................................................................................................................................................................................................................................................CTTGTGGGCGTTAGATTGGTCGT.........................................................................................................	23	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................................................................................TTGGTCGTGGCAACATTTTTAAACAGA......................................................................................	27	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................................................................................................................................................TATCTCTGGAAGCTACATGCTTAATC.............................................	26	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................................................................................TTGGTCGTGGCAACATTTTTAAACAGACT....................................................................................	29	1	1.00	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................................................................................................GTTAGATTGGTCGTGGCAACATTTT..............................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................................................................................................GTTAGATTGGTCGTGGCAAC...................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................TAAACAGACATGCGCTGCGTCTAT......................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0

droSec2

TGG---G----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CGTGGCAAAAAGTTTTTT-GGCAAATCGATAGTAATGTATAAGACTAAGAAAACTATGA-----------------AAAAATATCGAAAAGTTTTTTAACAA-----T	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
.......................................................................................................................................................................................................................................................................................................................AAAAAGTTTTTT-GGCAAATCG................................................................................	21	20	0.05	1	0	1	0	0

droYak3

GCA---CACTAAAA--CCATGATGGAT*---ACGCTATAGTCGAGTTCCCCGAC--T----------------------------------------------------------------ATCTGATACCCGTTACTCAGC---------------------------------------TAGTGTAAGTGCG----------------AAGGAC---------------------------------------------------------AGTTTTTGGCGGTTTGTGGGCGTTAGAGTGGGCGTGGACAAAAGTTTTTT-GGCAAATAGATAGAAATTTACAAGACTAATACAAAAATGA-----------------AAAAA----------TATCAAAACAT-----T

Size

Hit Count

Total Norm

Total

M026
Head

M043
Female-body

M056
Embryo

V046
Embryo

V052
Head

V058
Head

V120
Male-body

SRR1275488
Male larvae

SRR1275486
Male prepupae

SRR1275484
Male prepupae

GSM1528802
follicle cells

No Reads

droEre2

TTT---TCAAAAGTG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGAC---------------------------------------------------------AGTTTTTGGCGGTTTGTGGGCGTTGAAGGTGGCGTGGCAAAAAGTTTTTT-AGCCAATCTATAG-ATTTTACAAGAT--ATATATTATT------TAAAAGTGG----TAAAA----------TATCACATTTT-----T	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
No Reads

dwi_1832

scf2_1100000004590:3177398-3177548 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-24.5	-24.4	-24.2