dwi_1790

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------################################################## TGATGACAAAATTGTAAATATAGTCGTAGGCAGGGAAAATAAATCGCTTTAACGGGGGTGGTGTGGTGAGAGTGAGGCTTGTGGTGAAAAACGGGTGTGATGTGGTGAAAAATGGTAAGCAAAACATGAGAAACCGGACTAAAGGCAAAGTTCAAATATTTTAAAATTTTGTGACTTTTATTCACATTAATTTTGTAATAGGCAAATCACTCAAATAAATCAAAAAAGAAGAATGCCGCCGAGCATCATAA ************************************************........((((.((((....(((..((((((.((.....((............)).....)).))))))....))).....)))).))))..**********************************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M020 head	V117 male body	V118 embryo	M045 female body
............................................................................................................................CATGAGAAACCGGACTAAA............................................................................................................	19	0	1	2.00	2	2	0	0	0
...........................................................................................................................ACATGAGAAACCGGACTAAA............................................................................................................	20	0	1	1.00	1	1	0	0	0
..................................................AACGGGGGTGGTGTGGTGAGAGT..................................................................................................................................................................................	23	0	1	1.00	1	0	0	1	0
...........................................................................................................................ACATGAGAAACCGGACTAA.............................................................................................................	19	0	1	1.00	1	1	0	0	0
....................................................CGGGGGTGGTGTGGTGAG.....................................................................................................................................................................................	18	0	1	1.00	1	0	1	0	0
..................................................................................................................GTAAGCAAAACATGAGAAACCGGACTA..............................................................................................................	27	0	1	1.00	1	0	0	0	1
.................................................................................................................................GAAACCGGACTAAAGGCA........................................................................................................	18	0	1	1.00	1	1	0	0	0
...........................................................................................................AAGAATGGTAAGCAAAGCATGAG.........................................................................................................................	23	2	1	1.00	1	0	0	1	0
....................................................................................................................................ACCGGACTAAAGGCAAAGT....................................................................................................	19	0	1	1.00	1	1	0	0	0
...........................................................................................................................ACATGAGAAACCGGACTAAAG...........................................................................................................	21	0	1	1.00	1	1	0	0	0
......................................................................................................................GCAAAACATGAGAAACCGGACTAC.............................................................................................................	24	1	1	1.00	1	1	0	0	0
....................................................................................TGAGAAAAGGGTGTGATGCGG..................................................................................................................................................	21	3	1	1.00	1	0	1	0	0
...........................................................................GGCCTGTGTTGAAAAACGCG............................................................................................................................................................	20	3	2	0.50	1	0	0	0	1
.........................................................GTGGGGTGGTTAGAGTGAGGG.............................................................................................................................................................................	21	3	3	0.33	1	0	1	0	0
...........................................................CGTGTGGTGTGAGTGAGG..............................................................................................................................................................................	18	2	9	0.11	1	0	1	0	0
.................................................TAACGGTAGTGGCGTGGTG.......................................................................................................................................................................................	19	3	11	0.09	1	0	0	0	1
............................................................................GTTTTTGGTGAAAACCGGGT...........................................................................................................................................................	20	3	20	0.05	1	0	0	0	1

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------##################################################
TGATGACAAAATTGTAAATATAGTCGTAGGCAGGGAAAATAAATCGCTTTAACGGGGGTGGTGTGGTGAGAGTGAGGCTTGTGGTGAAAAACGGGTGTGATGTGGTGAAAAATGGTAAGCAAAACATGAGAAACCGGACTAAAGGCAAAGTTCAAATATTTTAAAATTTTGTGACTTTTATTCACATTAATTTTGTAATAGGCAAATCACTCAAATAAATCAAAAAAGAAGAATGCCGCCGAGCATCATAA
**************************************************........((((.((((....(((..((((((.((.....((............)).....)).))))))....))).....)))).))))..************************************************************************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M020
head

V117
male body

V118
embryo

M045
female body

............................................................................................................................CATGAGAAACCGGACTAAA............................................................................................................

2.00

...........................................................................................................................ACATGAGAAACCGGACTAAA............................................................................................................

1.00

..................................................AACGGGGGTGGTGTGGTGAGAGT..................................................................................................................................................................................

1.00

...........................................................................................................................ACATGAGAAACCGGACTAA.............................................................................................................

1.00

....................................................CGGGGGTGGTGTGGTGAG.....................................................................................................................................................................................

1.00

..................................................................................................................GTAAGCAAAACATGAGAAACCGGACTA..............................................................................................................

1.00

.................................................................................................................................GAAACCGGACTAAAGGCA........................................................................................................

1.00

...........................................................................................................AAGAATGGTAAGCAAAGCATGAG.........................................................................................................................

1.00

....................................................................................................................................ACCGGACTAAAGGCAAAGT....................................................................................................

1.00

...........................................................................................................................ACATGAGAAACCGGACTAAAG...........................................................................................................

1.00

......................................................................................................................GCAAAACATGAGAAACCGGACTAC.............................................................................................................

1.00

....................................................................................TGAGAAAAGGGTGTGATGCGG..................................................................................................................................................

1.00

...........................................................................GGCCTGTGTTGAAAAACGCG............................................................................................................................................................

0.50

.........................................................GTGGGGTGGTTAGAGTGAGGG.............................................................................................................................................................................

0.33

...........................................................CGTGTGGTGTGAGTGAGG..............................................................................................................................................................................

0.11

.................................................TAACGGTAGTGGCGTGGTG.......................................................................................................................................................................................

0.09

............................................................................GTTTTTGGTGAAAACCGGGT...........................................................................................................................................................

0.05

ACTACTGTTTTAACATTTATATCAGCATCCGTCCCTTTTATTTAGCGAAATTGCCCCCACCACACCACTCTCACTCCGAACACCACTTTTTGCCCACACTACACCACTTTTTACCATTCGTTTTGTACTCTTTGGCCTGATTTCCGTTTCAAGTTTATAAAATTTTAAAACACTGAAAATAAGTGTAATTAAAACATTATCCGTTTAGTGAGTTTATTTAGTTTTTTCTTCTTACGGCGGCTCGTAGTATT **********************************************************************************************************........((((.((((....(((..((((((.((.....((............)).....)).))))))....))).....)))).))))..************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M045 female body
.................................................................CACTCTCACTCCGAACACCACTTTT.................................................................................................................................................................	25	0	1	1.00	1	1
....................................................................TCTCACTCCGCACTCCACG....................................................................................................................................................................	19	3	19	0.05	1	1

ACTACTGTTTTAACATTTATATCAGCATCCGTCCCTTTTATTTAGCGAAATTGCCCCCACCACACCACTCTCACTCCGAACACCACTTTTTGCCCACACTACACCACTTTTTACCATTCGTTTTGTACTCTTTGGCCTGATTTCCGTTTCAAGTTTATAAAATTTTAAAACACTGAAAATAAGTGTAATTAAAACATTATCCGTTTAGTGAGTTTATTTAGTTTTTTCTTCTTACGGCGGCTCGTAGTATT
************************************************************************************************************........((((.((((....(((..((((((.((.....((............)).....)).))))))....))).....)))).))))..**************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M045
female body

.................................................................CACTCTCACTCCGAACACCACTTTT.................................................................................................................................................................

1.00

....................................................................TCTCACTCCGCACTCCACG....................................................................................................................................................................

0.05

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droWil2	scf2_1100000004590:1583214-1583464 +	dwi_1790	TGATGAC--------AAAATTGTAAATATAGTCGTAGGCAGGGAAAATAAATCGCTTTAACGGGGGTGGTGTGGTGAGAGTGAGGCTTGTGGTGAAAAACGGGTGTGATGTGGTGAAAAATGGTAAGCAAAACATGAGAAACCGGACTAAAGGCAAAGTTCAAATATTTTAAAATTTTGTGACTTTTATTCACATTAATTTTGTAATAGGCAAATCACTCAAATAAATCAAAAAAGAAGAATGCCGCCGAGCATCATAA
droMoj3	scaffold_6498:3168191-3168211 -		TAATAAC--------GAAAACGCAAATAT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droKik1	scf7180000302307:93209-93240 +		GAGTTACCAAAGCTAAAAATTGTAATTATAGT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droFic1	scf7180000453158:1274-1274 -		T------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droBia1	scf7180000302060:20545-20571 +		TGCGAAA---------GGGATGGAAATGTATTCATA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droWil2

TGATGAC--------AAAATTGTAAATATAGTCGTAGGCAGGGAAAATAAATCGCTTTAACGGGGGTGGTGTGGTGAGAGTGAGGCTTGTGGTGAAAAACGGGTGTGATGTGGTGAAAAATGGTAAGCAAAACATGAGAAACCGGACTAAAGGCAAAGTTCAAATATTTTAAAATTTTGTGACTTTTATTCACATTAATTTTGTAATAGGCAAATCACTCAAATAAATCAAAAAAGAAGAATGCCGCCGAGCATCATAA	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
....................................................................................................................................CATGAGAAACCGGACTAAA............................................................................................................	19	1	2.00	2	2	0	0	0	0
...................................................................................................................................ACATGAGAAACCGGACTAAA............................................................................................................	20	1	1.00	1	1	0	0	0	0
..........................................................AACGGGGGTGGTGTGGTGAGAGT..................................................................................................................................................................................	23	1	1.00	1	0	0	0	1	0
...................................................................................................................................ACATGAGAAACCGGACTAA.............................................................................................................	19	1	1.00	1	1	0	0	0	0
............................................................CGGGGGTGGTGTGGTGAG.....................................................................................................................................................................................	18	1	1.00	1	0	0	1	0	0
..........................................................................................................................GTAAGCAAAACATGAGAAACCGGACTA..............................................................................................................	27	1	1.00	1	0	1	0	0	0
.........................................................................................................................................GAAACCGGACTAAAGGCA........................................................................................................	18	1	1.00	1	1	0	0	0	0
............................................................................................................................................ACCGGACTAAAGGCAAAGT....................................................................................................	19	1	1.00	1	1	0	0	0	0
...................................................................................................................................ACATGAGAAACCGGACTAAAG...........................................................................................................	21	1	1.00	1	1	0	0	0	0
..............................................................................................................................GCAAAACATGAGAAACCGGACTAC.............................................................................................................	24	1	1.00	1	1	0	0	0	0

droMoj3

TAATAAC--------GAAAACGCAAATAT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
No Reads

droKik1

GAGTTACCAAAGCTAAAAATTGTAATTATAGT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droFic1

T------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droBia1

TGCGAAA---------GGGATGGAAATGTATTCATA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

dwi_1790

scf2_1100000004590:1583264-1583414 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads