ID:

dwi_1772

 Coordinate:

scf2_1100000004590:737960-738110 -

 Confidence:

Candidate

 Type:

Unknown

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-9.6 -9.6 -9.6

Flybase annnotation

exon [dwil_GLEANR_16643:1]; CDS [Dwil\GK16268-cds]; intron [Dwil\GK16268-in]

No Repeatable elements found

mature
  1. scf2_1100000004511:321295-321313 +
  2. scf2_1100000004540:602841-602859 -
  3. scf2_1100000004585:6051907-6051925 -
  4. dwi_1772  scf2_1100000004590:738066-738084 -
  5. scf2_1100000004902:310219-310237 +
  6. scf2_1100000004909:9709539-9709557 -
  7. scf2_1100000004954:2481873-2481891 -
  8. scf2_1100000004963:4161421-4161439 +
star

Sense Strand Reads

##################################################---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
AATGAAAATGGAATTCGATCAAGATACACCTGAAGATCAACACCCAAAATGTATGACCGAAGCGCAGTAGTTAAAAAAAAAAAACAAAGAAAAGAAAAACACGAAAATTTACTCAAATTTCGAAAGGCATATGAAATTGAATTGAAAAATGCATTCAACATTCAACAAAACTAAATTAAAACTGAATCACACCAAAGCCATGAAGTTTGTCCTGATTAGCTTCATTTGGCCGCTCGTCTCGTTGGTTTTTT
**************************************************(((((.((..(((......))).............................(((((.(((....))))))))...)))))))....((((((((((.......))))..))))))....................................************************************************** 		Read size # Mismatch Hit Count Total Norm Total V118
embryo
 M045
female body
 V119
head
......................................................................................AAGAAAGGAGAAACACGAAAA................................................................................................................................................ 21 2 4 0.25 1 1 0 0
........................................................................................................................................................................................AATCACAGCAAAGCCATTA................................................ 19 2 20 0.15 3 2 0 1
............................................................................AAAAAAAACAAAGAAAAGA............................................................................................................................................................ 19 0 8 0.13 1 1 0 0
.........................................................................................................................................................................................................................AACTTCATTTGGCCAATCGTC............. 21 3 8 0.13 1 1 0 0
................................................................................AAAACAAAGAAAAGAAAAA........................................................................................................................................................ 19 0 14 0.07 1 1 0 0
.................................................................................AAACAAAGAGAAGAAAAACA...................................................................................................................................................... 20 1 19 0.05 1 1 0 0
................................................................GAGTAGTTAAAAAAAAAA......................................................................................................................................................................... 18 1 20 0.05 1 1 0 0
...................................................................................................................................................................................................................................GGCCCCAGGTCTCGTTGG...... 18 3 20 0.05 1 0 1 0
............................................................TGGGCAGTAGTTAAAAAA............................................................................................................................................................................. 18 2 20 0.05 1 1 0 0

Anti-sense strand reads

TTACTTTTACCTTAAGCTAGTTCTATGTGGACTTCTAGTTGTGGGTTTTACATACTGGCTTCGCGTCATCAATTTTTTTTTTTTGTTTCTTTTCTTTTTGTGCTTTTAAATGAGTTTAAAGCTTTCCGTATACTTTAACTTAACTTTTTACGTAAGTTGTAAGTTGTTTTGATTTAATTTTGACTTAGTGTGGTTTCGGTACTTCAAACAGGACTAATCGAAGTAAACCGGCGAGCAGAGCAACCAAAAAA
**************************************************(((((.((..(((......))).............................(((((.(((....))))))))...)))))))....((((((((((.......))))..))))))....................................************************************************** 		Read size # Mismatch Hit Count Total Norm Total V117
male body		 V118
embryo
 V119
head
 M045
female body
....................................................................................................................................................................................TCAATTGGTGTGGTTTCGGTA.................................................. 21 3 8 0.13 1 1 0 0 0
...............................CATCTAGTTGGGGGTTTCA......................................................................................................................................................................................................... 19 3 18 0.06 1 0 1 0 0
......................................................................................................................................................................................ACTTGGTGTGATTTCGGTAT................................................. 20 3 19 0.05 1 0 0 1 0
.............................................................................................CTTTGTGTGCTTTTGAGTGAG......................................................................................................................................... 21 3 20 0.05 1 0 0 0 1

Re-alignment of all predicted orthologs

Species Coordinate ID Alignment
droWil2 scf2_1100000004590:737910-738160 - dwi_1772 AATGAAAATGGAATTCGATCAAGATACACCTGAAGATCAACACCCAAAATGTATGACCGAAGCGCAGTAGTTAAAAAAAAAAAACAAAGAAAAGAAAAACACGAAAATTTACTCAAATTTCGAAAGGCATATGAAATTGAATTGAAAAATGCATTCAACATTCAACAAAACTAAATTAAAACTGAATCACACCAAAGCCATGAAGTTTGTCCTGATTAGCTTCATTTGGCCGCTCGTCTCGTTGGTTTTTT
droMoj3 scaffold_6473:11593714-11593761 + A------------------------------------------------------------------------AAAAAAAAAAAAAAAGAAGCAGAAAATGTAAAAAATTGCCCA-----------------------------------------------------------------------------------------------------------------------------------TTTTT
droAna3 scaffold_13417:1847867-1847893 - A------------------------------------------------------------------------AAAAAAAAAAACAAAAAAAAAAAAAA--------------------------------------------------------------------------------------------------------------------------------------------------------
droBip1 scf7180000396484:92358-92389 - T-------------------------------------------------------------------AATTAAAAAAAAAACAAAAGGAAAAAGAAAA--------------------------------------------------------------------------------------------------------------------------------------------------------
droEug1 scf7180000409230:428465-428509 - GCAGGAACTGAAGGCAAACCAAGATCAAGA------------------------------------------------------TCTGGAGAAGATCAA--------------------------------------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droWil2
AATGAAAATGGAATTCGATCAAGATACACCTGAAGATCAACACCCAAAATGTATGACCGAAGCGCAGTAGTTAAAAAAAAAAAACAAAGAAAAGAAAAACACGAAAATTTACTCAAATTTCGAAAGGCATATGAAATTGAATTGAAAAATGCATTCAACATTCAACAAAACTAAATTAAAACTGAATCACACCAAAGCCATGAAGTTTGTCCTGATTAGCTTCATTTGGCCGCTCGTCTCGTTGGTTTTTTSizeHit CountTotal NormTotalM020
Head		M045
Female-body		V117
Male-body		V118
Embryo		V119
Head
............................................................................AAAAAAAACAAAGAAAAGA............................................................................................................................................................1980.13100010
................................................................................AAAACAAAGAAAAGAAAAA........................................................................................................................................................19140.07100010
droMoj3
A------------------------------------------------------------------------AAAAAAAAAAAAAAAGAAGCAGAAAATGTAAAAAATTGCCCA-----------------------------------------------------------------------------------------------------------------------------------TTTTTSizeHit CountTotal NormTotalM046
Female-body		V041
Embryo		V049
Head		V056
Head		M060
Embryo		V110
Male-body
No Reads
droAna3
A------------------------------------------------------------------------AAAAAAAAAAACAAAAAAAAAAAAAA--------------------------------------------------------------------------------------------------------------------------------------------------------SizeHit CountTotal NormTotalM044
Female-body		M058
Embryo		V039
Embryo		V055
Head		V105
Male-body		V106
Head
..........................................................................AAAAAAAAAACAAAAAAAAAAAAA.........................................................................................................................................................24200.153030000
..............................................................................AAAAAACAAAAAAAAAAAAAA--------------------------------------------------------------------------------------------------------------------------------------------------------21200.051001000
droBip1
T-------------------------------------------------------------------AATTAAAAAAAAAACAAAAGGAAAAAGAAAA--------------------------------------------------------------------------------------------------------------------------------------------------------SizeHit CountTotal NormTotal
No Reads
droEug1
GCAGGAACTGAAGGCAAACCAAGATCAAGA------------------------------------------------------TCTGGAGAAGATCAA--------------------------------------------------------------------------------------------------------------------------------------------------------SizeHit CountTotal NormTotal
No Reads

Generated: 05/18/2015 at 02:27 AM