ID:

dwi_171

 Coordinate:

scf2_1100000004909:1575922-1575999 +

 Confidence:

confident

 Class:

Canonical miRNA

 Genomic Locale:

intron

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-37.8 -37.7 -37.6

Flybase annnotation

intron [Dwil\GK25487-in]

Repeatable elements

NameClassFamilyStrand
AT_richLow_complexityLow_complexity+

Sense Strand Reads

TGAAGTCTAAAATTAAAGTTTAAGCACAAACGTAGATGGTCCAGACCTCATTCAGAATTTTGGGATTTGTTTTGCCCTTTTTGTTGTTTATTTAATATTAAAAAAAAGTCCTAAGATCCTGAATGAGGTCAGGTTCAGAATGGCTACTATCATTCTAGGTCGATTGCACTTATTTCAT

***********************************.(((.((.((((((((((((.((((((((((((.((((........(((((......)))))...)))).)))))))))))).)))))))))))).)).))).....************************************ 		Read size # Mismatch Hit Count Total Norm Total V119

head

 M020

head

 V118

embryo

 V117

male body		 M045

female body
........................................................................................................AAAGTCCTAAGATCCTGAATGAG................................................... 23 0 1 80.00 80 37 19 21 2 1
........................................................................................................AAAGTCCTAAGATCCTGAATGA.................................................... 22 0 1 9.00 9 5 3 1 0 0
..........................................................................................................AGTCCTAAGATCCTGAATGAGT.................................................. 22 1 1 3.00 3 1 1 1 0 0
..........................................................................................................AGTCCTAAGATCCTGAATGAG................................................... 21 0 1 3.00 3 1 2 0 0 0
.......................................................................................................AAAAGTCCTAAGATCCTGAATG..................................................... 22 0 1 3.00 3 1 2 0 0 0
.......................................................................................................AAAAGTCCTAAGATCCTGAAT...................................................... 21 0 1 2.00 2 0 2 0 0 0
........................................................................................................AAAGTCCTAAGATCCTGAATG..................................................... 21 0 1 2.00 2 1 1 0 0 0
..................................................TTCAGAATTTTGGGATTTGTTT.......................................................................................................... 22 0 1 2.00 2 0 0 2 0 0
.......................................................................................................AAAAGTCCTAAGATCCTGAATGA.................................................... 23 0 1 2.00 2 0 0 2 0 0
..........................................................................................................AGTCCTAAGATCCTGAATGAGGT................................................. 23 0 1 2.00 2 0 2 0 0 0
........................................................................................................AAAGTCCTAAGATCCTGAATGAA................................................... 23 1 1 1.00 1 1 0 0 0 0
..................................................TTCAGAATTTTGGGATTTGTT........................................................................................................... 21 0 1 1.00 1 0 0 0 1 0
........................................................................................................AAAGTCCTAATATCCTGAATGAG................................................... 23 1 1 1.00 1 1 0 0 0 0
........................................................................................................AAAGTCCTAAGATCCTGAATGAGT.................................................. 24 1 1 1.00 1 1 0 0 0 0
...........................................................................................................GTCCTAAGATCCTGAATGAGGT................................................. 22 0 1 1.00 1 0 0 1 0 0
..........................................................................................................AGTCCTAAGATCCTGAATGAGA.................................................. 22 1 1 1.00 1 1 0 0 0 0
..........................................................................................................AGTCCTAAGATCCTGAATGA.................................................... 20 0 1 1.00 1 1 0 0 0 0
..........................................................................................................AGTCCTAAGATCCTGAATGAGGG................................................. 23 1 1 1.00 1 0 1 0 0 0
........................................................................................................AAAGTCCTAAGATCCTGAAT...................................................... 20 0 1 1.00 1 0 1 0 0 0
........................................................................................................AAAGTCCTAAGATCTTGAATGAG................................................... 23 1 1 1.00 1 1 0 0 0 0
........................................................................................................TAAGTCTTAAGATCCTGAATGAG................................................... 23 2 1 1.00 1 1 0 0 0 0
........................................................................................................GAAGTCCTAAGATCCTGAATGAG................................................... 23 1 1 1.00 1 1 0 0 0 0
........................................................................................................AAAGTCCTAAGATCCTGAATAAG................................................... 23 1 1 1.00 1 0 0 1 0 0
........................................................................................................AAAGTCCTAAGATCCTGAATGAGA.................................................. 24 1 1 1.00 1 1 0 0 0 0
.......................................................................................................AAAAGTCCTAAGATCCTGAATGAG................................................... 24 0 1 1.00 1 0 0 1 0 0
........................................................................................................AAAGTCCTAAGATCCTGAATGG.................................................... 22 1 1 1.00 1 0 1 0 0 0
........................................................................................................AAAGTCCTAACATCCTGAATGAG................................................... 23 1 1 1.00 1 0 0 1 0 0
............................................................................CTTTTTGTTGTTTATTTA.................................................................................... 18 0 2 0.50 1 0 0 1 0 0
...................................................................................................................ATCCTGGATGAGGTCAAGTT........................................... 20 2 3 0.33 1 0 0 0 0 1
.........................................................................................................AAATCAGAAGATCCTGAATGAG................................................... 22 3 5 0.20 1 0 0 0 1 0
..........................................................................................................AGTCCGACGATCCTGAAT...................................................... 18 2 11 0.18 2 0 0 0 0 2
.......................................................................................................................TGTATGTGATCAGGTTCAG........................................ 19 3 20 0.05 1 0 0 0 0 1
...........................................................................................................................TGAGGTCGGGTCCAGAGTG.................................... 19 3 20 0.05 1 0 0 0 0 1
..............................................................................................................................GGTAAGGTTCATAACGGC.................................. 18 3 20 0.05 1 0 0 0 0 1

Anti-sense strand reads

ACTTCAGATTTTAATTTCAAATTCGTGTTTGCATCTACCAGGTCTGGAGTAAGTCTTAAAACCCTAAACAAAACGGGAAAAACAACAAATAAATTATAATTTTTTTTCAGGATTCTAGGACTTACTCCAGTCCAAGTCTTACCGATGATAGTAAGATCCAGCTAACGTGAATAAAGTA

************************************.(((.((.((((((((((((.((((((((((((.((((........(((((......)))))...)))).)))))))))))).)))))))))))).)).))).....*********************************** 		Read size # Mismatch Hit Count Total Norm Total M020

head

 V117

male body		 V119

head
...........................................................................................................CAGGCTTCTAGGCCTTACTA................................................... 20 3 3 0.33 1 1 0 0
.........................................................................................................TCCAGGCTTCTAGGCCTTAC..................................................... 20 3 3 0.33 1 1 0 0
.........................................................................................................TCCAGGCTTCTAGGCCTTA...................................................... 19 3 6 0.17 1 1 0 0
.................CCAAATCGTGCTTGCATCTA............................................................................................................................................. 20 3 7 0.14 1 1 0 0
.................................TCTACAAGGTATGGAGTC............................................................................................................................... 18 3 20 0.05 1 0 0 1
.........................................................................................................TCCAGGTTTCTAGGCCTTAC..................................................... 20 3 20 0.05 1 0 1 0

Re-alignment of all predicted orthologs

Species Coordinate ID Type Alignment
droWil2 scf2_1100000004909:1575872-1576049 + dwi_171 confident TGAAGTCTAAAATTAAAGTTTAAGCACAAACGTAGATGGTCCAGACCTCATTCAGAATTTTGGGATTTGTTTTGCCCTTTTTGTTGTTTATTTAATATTAAAAAAAAGTCCTAAGATCCTGAATGAGGTCAGGTTCAGAATGGCTACTATCATTCTAGGTCGATTGCACTTATTTCAT
droKik1 scf7180000299665:440-440 - ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------T

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droWil2
TGAAGTCTAAAATTAAAGTTTAAGCACAAACGTAGATGGTCCAGACCTCATTCAGAATTTTGGGATTTGTTTTGCCCTTTTTGTTGTTTATTTAATATTAAAAAAAAGTCCTAAGATCCTGAATGAGGTCAGGTTCAGAATGGCTACTATCATTCTAGGTCGATTGCACTTATTTCATSizeHit CountTotal NormTotalM020

Head		M045

Female-body		V117

Male-body		V118

Embryo		V119

Head
........................................................................................................AAAGTCCTAAGATCCTGAATGAG...................................................23180.008019122137
........................................................................................................AAAGTCCTAAGATCCTGAATGA....................................................2219.00930015
..........................................................................................................AGTCCTAAGATCCTGAATGAGT..................................................2213.00310011
..........................................................................................................AGTCCTAAGATCCTGAATGAG...................................................2113.00320001
.......................................................................................................AAAAGTCCTAAGATCCTGAATG.....................................................2213.00320001
.......................................................................................................AAAAGTCCTAAGATCCTGAAT......................................................2112.00220000
........................................................................................................AAAGTCCTAAGATCCTGAATG.....................................................2112.00210001
..................................................TTCAGAATTTTGGGATTTGTTT..........................................................................................................2212.00200020
.......................................................................................................AAAAGTCCTAAGATCCTGAATGA....................................................2312.00200020
..........................................................................................................AGTCCTAAGATCCTGAATGAGGT.................................................2312.00220000
........................................................................................................AAAGTCCTAAGATCCTGAATGAA...................................................2311.00100001
..................................................TTCAGAATTTTGGGATTTGTT...........................................................................................................2111.00100100
........................................................................................................AAAGTCCTAAGATCCTGAATGAGT..................................................2411.00100001
...........................................................................................................GTCCTAAGATCCTGAATGAGGT.................................................2211.00100010
..........................................................................................................AGTCCTAAGATCCTGAATGAGA..................................................2211.00100001
..........................................................................................................AGTCCTAAGATCCTGAATGA....................................................2011.00100001
..........................................................................................................AGTCCTAAGATCCTGAATGAGGG.................................................2311.00110000
........................................................................................................AAAGTCCTAAGATCCTGAAT......................................................2011.00110000
........................................................................................................AAAGTCCTAAGATCCTGAATGAGA..................................................2411.00100001
.......................................................................................................AAAAGTCCTAAGATCCTGAATGAG...................................................2411.00100010
........................................................................................................AAAGTCCTAAGATCCTGAATGG....................................................2211.00110000
............................................................................CTTTTTGTTGTTTATTTA....................................................................................1820.50100010
droKik1
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TSizeHit CountTotal NormTotal
No Reads

Generated: 10/20/2015 at 06:58 PM