ID:

dwi_1361

Coordinate:

scf2_1100000004521:11588423-11588573 -

Confidence:

Candidate

Type:

Unknown

[View on UCSC Genome Browser {Cornell Mirror}]

Legend:

mature

star

mismatch in alignment

mismatch in read

Predicted structure

-8.7	-8.2	-8.0	-7.9

Flybase annnotation

CDS [Dwil\GK14572-cds]; exon [dwil_GLEANR_14887:3]; intron [Dwil\GK14572-in]

No Repeatable elements found

Sense Strand Reads

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------#####################----------------------------- CGATTGTCACTACAGCTGGAATGTCCCATGGTGGTATGTGGCCTCCATTGCCCATAAATTTGGATGCACTTTTGAAGTTCCAAAAAAAAGACTTACAATGTATTATGATGATGATGATGATGTTAAACACACAAATGACTGGTTGATTCTTCCACATTTTGATGATTCAACTGGCCAAAATAAACATCCATATGACCTTAGCATACTAACGGCCAGTTCTAAGTATGAACGAGATGTAAGCCCTCGGATGC ****************************************************.............(((.....((((...............(((...((((((....))))))....))).............)))))))**********************************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V118 embryo	M045 female body	V117 male body
.....................................................................................................................................................................................................................................CGAGATGTAAGCCCTCGGA...	19	0	1	1.00	1	1	0	0
.......CACTACAGCTGGAATGTCC.................................................................................................................................................................................................................................	19	0	1	1.00	1	1	0	0
.....................................................................................................................TGATGTTAAACACACAAATGACTGGT............................................................................................................	26	0	1	1.00	1	0	1	0
.........CTACAGCTGGAATGTCCCATGGTG..........................................................................................................................................................................................................................	24	0	1	1.00	1	1	0	0
...................................................................................................................................................TCTTCGACTCTTTGATGATTCA..................................................................................	22	3	1	1.00	1	0	1	0
..........................................................................................................................................................................................................................CTAAGTATGAACGAGATGTA.............	20	0	1	1.00	1	0	0	1
......................................................TAAATTTGGATGCACTTTTGAAGT.............................................................................................................................................................................	24	0	1	1.00	1	0	1	0
.....................TGTCCAATGGCGGCATGTGGC.................................................................................................................................................................................................................	21	3	3	0.33	1	0	0	1
...................................................................................................................................CAAATGGCTGGTTGTTTCTCC...................................................................................................	21	3	6	0.17	1	0	0	1
........................................................................................................ATGATGATGATGATGATGTTA..............................................................................................................................	21	0	18	0.11	2	2	0	0
.......................................................................................................................................TACCTGGTTGATTCTGCCA.................................................................................................	19	3	20	0.10	2	1	1	0

Anti-sense strand reads

GCTAACAGTGATGTCGACCTTACAGGGTACCACCATACACCGGAGGTAACGGGTATTTAAACCTACGTGAAAACTTCAAGGTTTTTTTTCTGAATGTTACATAATACTACTACTACTACTACAATTTGTGTGTTTACTGACCAACTAAGAAGGTGTAAAACTACTAAGTTGACCGGTTTTATTTGTAGGTATACTGGAATCGTATGATTGCCGGTCAAGATTCATACTTGCTCTACATTCGGGAGCCTACG **********************************************************************************************************.............(((.....((((...............(((...((((((....))))))....))).............)))))))****************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M045 female body	V119 head
......................................................................................TTTCTGATTGTTAAATAATAC................................................................................................................................................	21	2	2	0.50	1	1	0
...................TTCCAGGATACCAGCATACA....................................................................................................................................................................................................................	20	3	5	0.20	1	0	1
..................................................................GTGGAAACTTCTAGGTTT.......................................................................................................................................................................	18	2	17	0.06	1	1	0
...................................................................TGAAAAGTCCAAGGTTTGTT....................................................................................................................................................................	20	3	20	0.05	1	1	0

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droWil2	scf2_1100000004521:11588373-11588623 -	dwi_1361	CGATTGTCACTACAGCTGGAATGTCCCATGGTGGTATGTGGCCTCCATTGCCCATAAATTTGGATGCACTTTTGAAG----------------------------TTCCAAAAAAAAGACTTACAATGTATTATGATGATGATGATGAT--------GTTAAACACACAAATGACTGGTTGATTCTTCC---ACATT--T------TGATGATTCAACTGGCCAAAATAAACATCCATATGACCTTAGCATACTAACGGCCAGTTCTAAGTATGAACGAGA---TGTAAGCCCTCGGATGC
dp5	2:10050625-10050860 -		AGACACACACTACAGCTGGAATGTCCCTTGGTGGTACGTCGCTGCAGTCGCGGATAAATTCGGGCACAAGTTCACC---------------------------TGTGCCA----------------CGGACCCTGACG------GTGAGCTGATCATGG--------ATGAGGACTACTCGTTTTC-GGACAACATG--TTGGGTTGGCTAATAGAACCAGCTAA--AAGCACCCAATTCGATCTGAGTTTGCTGACATCATCCAGCCAGTACGAGAAGAG---GGTCAGCAAAAGCAAGC
droPer2	scaffold_0:10431248-10431483 +		AGACACACACTACAGCTGGAATGTCCCATGGTGGTACGTCGCTGCAGTCGCGGATAAATTCGGGCACAAGTTCACC---------------------------TGTGCCA----------------CAGACCCTGACG------GTGAGCTGATCATGG--------ATGAGGACTACTCGTTTTC-GGACAACATG--TTGGGTTGGCTAATTGAACCAGCTAA--AAGCACCCAATTCGATCTGAGTTTGCTGACATCATCCAGCCAGTACGAGAAGAG---GGTCAGCAAAAGCAAGC
droAna3	scaffold_12943:2341743-2341966 -		TGACGTGCATTACAGCTGGAATACACCATGGTGGTACGTTGCAGCAATAGCAGAAAAGTTCGGCCACAAATTTGTAT---------------------------GTTCTA----------------CACCTGCCAATG------GTGAACTAATATTGG--------ATGGGGATTATTCGGAATC-TAACAACTTG--ATCGGATGGCTAATAGAGTCAGGCCA--CAGTGCGCAATTTGACCTAAGCTTACTAACGTCATCGTGTCAGTACGAGACGAG---------------AAAAC
droKik1	scf7180000301798:455-663 -		AAAGACACACTTTAGCGGTAATGTTCCTTGGTGGTTTGTTGGGGCTATTGCTGCCACATTTGGGCAAAACTTCAAAG---------------------------------------------------------------------------------T--------AAAAGCACGCGTCGATTTT-AAACAAAATGAAATAGGATGGCGGATAAATGCAGAAAA--TAACGAGCAATATGATCTAGGCATTCTTACTGCGTCCAGCAAGTACGAACAGAAAGTGTTAAGGAGTCGTGGGC
droTak1	scf7180000413017:216956-217082 +		AAAAACACATTACAGCGGCAATGTTCCTTGGTGGTATGTGGCCACTATTGCCTCTAAATTCGGTCATACTATTAAAATAGAGGCATCTTCCCAAGAAGATTATAGTCCCA----------------ATGGTTTTGAAG------ATGAT--------------------------------------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droWil2

CGATTGTCACTACAGCTGGAATGTCCCATGGTGGTATGTGGCCTCCATTGCCCATAAATTTGGATGCACTTTTGAAG----------------------------TTCCAAAAAAAAGACTTACAATGTATTATGATGATGATGATGAT--------GTTAAACACACAAATGACTGGTTGATTCTTCC---ACATT--T------TGATGATTCAACTGGCCAAAATAAACATCCATATGACCTTAGCATACTAACGGCCAGTTCTAAGTATGAACGAGA---TGTAAGCCCTCGGATGC	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
....................................................................................................................................................................................................................................................................................CGAGA---TGTAAGCCCTCGGA...	19	1	1.00	1	0	0	0	1	0
.......CACTACAGCTGGAATGTCC...................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	1	0
.................................................................................................................................................TGAT--------GTTAAACACACAAATGACTGGT..........................................................................................................................	26	1	1.00	1	0	1	0	0	0
.........CTACAGCTGGAATGTCCCATGGTG............................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	1	0
.........................................................................................................................................................................................................................................................................CTAAGTATGAACGAGA---TGTA.............	20	1	1.00	1	0	0	1	0	0
......................................................TAAATTTGGATGCACTTTTGAAG----------------------------T...................................................................................................................................................................................................	24	1	1.00	1	0	1	0	0	0
....................................................................................................................................ATGATGATGATGATGAT--------GTTA............................................................................................................................................	21	18	0.11	2	0	0	0	2	0

dp5

AGACACACACTACAGCTGGAATGTCCCTTGGTGGTACGTCGCTGCAGTCGCGGATAAATTCGGGCACAAGTTCACC---------------------------TGTGCCA----------------CGGACCCTGACG------GTGAGCTGATCATGG--------ATGAGGACTACTCGTTTTC-GGACAACATG--TTGGGTTGGCTAATAGAACCAGCTAA--AAGCACCCAATTCGATCTGAGTTTGCTGACATCATCCAGCCAGTACGAGAAGAG---GGTCAGCAAAAGCAAGC	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
..............................................................................................................................................................................................................................................TCGATCTGAGTTTGCTGACA...........................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.............................................................................................................A----------------CGGACCCTGACG------GTGAGCT......................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................CGATCTGAGTTTGCTGACAT..........................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0

droPer2

AGACACACACTACAGCTGGAATGTCCCATGGTGGTACGTCGCTGCAGTCGCGGATAAATTCGGGCACAAGTTCACC---------------------------TGTGCCA----------------CAGACCCTGACG------GTGAGCTGATCATGG--------ATGAGGACTACTCGTTTTC-GGACAACATG--TTGGGTTGGCTAATTGAACCAGCTAA--AAGCACCCAATTCGATCTGAGTTTGCTGACATCATCCAGCCAGTACGAGAAGAG---GGTCAGCAAAAGCAAGC	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
No Reads

droAna3

TGACGTGCATTACAGCTGGAATACACCATGGTGGTACGTTGCAGCAATAGCAGAAAAGTTCGGCCACAAATTTGTAT---------------------------GTTCTA----------------CACCTGCCAATG------GTGAACTAATATTGG--------ATGGGGATTATTCGGAATC-TAACAACTTG--ATCGGATGGCTAATAGAGTCAGGCCA--CAGTGCGCAATTTGACCTAAGCTTACTAACGTCATCGTGTCAGTACGAGACGAG---------------AAAAC	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
No Reads

droKik1

AAAGACACACTTTAGCGGTAATGTTCCTTGGTGGTTTGTTGGGGCTATTGCTGCCACATTTGGGCAAAACTTCAAAG---------------------------------------------------------------------------------T--------AAAAGCACGCGTCGATTTT-AAACAAAATGAAATAGGATGGCGGATAAATGCAGAAAA--TAACGAGCAATATGATCTAGGCATTCTTACTGCGTCCAGCAAGTACGAACAGAAAGTGTTAAGGAGTCGTGGGC	Size	Hit Count	Total Norm	Total
No Reads

droTak1

AAAAACACATTACAGCGGCAATGTTCCTTGGTGGTATGTGGCCACTATTGCCTCTAAATTCGGTCATACTATTAAAATAGAGGCATCTTCCCAAGAAGATTATAGTCCCA----------------ATGGTTTTGAAG------ATGAT--------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

Generated: 05/18/2015 at 01:17 AM