ID:

dwi_135

 Coordinate:

scf2_1100000004909:7153734-7153792 -

 Confidence:

candidate

 Class:

Canonical miRNA

 Genomic Locale:

CDS

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-34.1

Flybase annnotation

CDS [Dwil\GK25170-cds]

No Repeatable elements found

Sense Strand Reads

CAATTGACATGAAGACAATTTTACTTTTGTGGATGTTTTGCGGATCCGGGTTGTTGTTGTTGCCAGCCTGGGCCGAACAAAATGCCAATGCAACAGCAACAGCAACTTCGGCTCCTGCTCCTGCTTCAGCTGCGGCTGCCGCTTCCAGCAATCCTGCCC

***********************************....(((((.(((((((((((((((((...((...(((..........)))...))..))))))))))))).)))).))).)).....************************************ 		Read size # Mismatch Hit Count Total Norm Total V119

head

 V118

embryo

 M045

female body

 V117

male body		 M020

head
..................................................TTGTTGTTGTTGCCAGCCTGGGC...................................................................................... 23 0 1 38.00 38 33 5 0 0 0
..................................................TTGTTGTTGTTGCCAGCCTGGG....................................................................................... 22 0 1 14.00 14 11 2 0 0 1
..................................................TTGTTGTTGTTGCCAGCCTGG........................................................................................ 21 0 1 3.00 3 2 0 1 0 0
...........................................ATCCGGGTTGTTGTTGTTGCC............................................................................................... 21 0 1 1.00 1 1 0 0 0 0
..................................................TTGTTGTTGTTGCCAGCCTAGGC...................................................................................... 23 1 1 1.00 1 1 0 0 0 0
..................................................TTGTTGTTGTTGACAGCCTGGGC...................................................................................... 23 1 1 1.00 1 1 0 0 0 0
.................................................GTTGTTGTTGTTGCCATCCTGGGCC..................................................................................... 25 1 1 1.00 1 0 1 0 0 0
..................................................TTGTTGTTGTTGCCAGCCTG......................................................................................... 20 0 1 1.00 1 0 0 1 0 0
..................................................TTGTTGTTGTTGCCAGCCTGGC....................................................................................... 22 1 1 1.00 1 1 0 0 0 0
....................................................................................................................GCTCCTGCTTCAGCTGCGGC....................... 20 0 1 1.00 1 0 0 1 0 0
.....................................................................................CAATGCAACAGCAACAGCAACTT................................................... 23 0 1 1.00 1 1 0 0 0 0
.....................................................TTGTTGTTGCCAGCCTGGGC...................................................................................... 20 0 1 1.00 1 1 0 0 0 0
....................................................GTTGTTGTTGCCAGCCTGGGC...................................................................................... 21 0 1 1.00 1 0 1 0 0 0
..................................................TTGTTGTTGTTGCCAGTCTGGG....................................................................................... 22 1 1 1.00 1 1 0 0 0 0
...........................................................................................AACAGCAACAGCAACTTCGGCTCCTG.......................................... 26 0 1 1.00 1 0 0 1 0 0
.....................................................................................................GCAACTTCGGCTCCTGCTCCTGCTTCA............................... 27 0 1 1.00 1 0 0 1 0 0
..................................................TTGTTGTTGTTGCAAGCCTGGGC...................................................................................... 23 1 1 1.00 1 1 0 0 0 0
..................................................TTGTTGTTGTTGCCAGCCTGGAC...................................................................................... 23 1 1 1.00 1 0 0 1 0 0
..................................GTTTTGCGGATCCGGGTT........................................................................................................... 18 0 1 1.00 1 0 0 1 0 0
..................................................TTGTTGTTGTTGCCAGCCTGGGT...................................................................................... 23 1 1 1.00 1 1 0 0 0 0
..................................................TTGTTGTTGTTGCCAGCCTGGGCCGT................................................................................... 26 1 1 1.00 1 0 0 1 0 0
..................................................TTGTTGTTGTTTCCAGCCTGGG....................................................................................... 22 1 1 1.00 1 1 0 0 0 0
...............................GATGTTTTGCGGATCCGGGTTGT......................................................................................................... 23 0 1 1.00 1 0 0 0 1 0
..................................................TTCTTGTTGTTGCCAGCCTGGGC...................................................................................... 23 1 1 1.00 1 1 0 0 0 0
.........................TTTGGGGATGTTTTGCGGATCCGGGTT........................................................................................................... 27 1 1 1.00 1 0 0 0 1 0
..................................................TTGTTGTTGTTGCCACCCTGGGC...................................................................................... 23 1 1 1.00 1 1 0 0 0 0
......................................GGCGGAACCGGGTTGGTGTT..................................................................................................... 20 3 5 0.20 1 0 0 0 1 0
..........................................GATCCGGGTTGAAGTCGTT.................................................................................................. 19 3 9 0.11 1 0 0 0 1 0

Anti-sense strand reads

GTTAACTGTACTTCTGTTAAAATGAAAACACCTACAAAACGCCTAGGCCCAACAACAACAACGGTCGGACCCGGCTTGTTTTACGGTTACGTTGTCGTTGTCGTTGAAGCCGAGGACGAGGACGAAGTCGACGCCGACGGCGAAGGTCGTTAGGACGGG

************************************....(((((.(((((((((((((((((...((...(((..........)))...))..))))))))))))).)))).))).)).....*********************************** 		Read size # Mismatch Hit Count Total Norm Total V118

embryo

 M045

female body

 V117

male body
.......................................CGCCTAGGCCCAACAACAAC.................................................................................................... 20 0 1 1.00 1 1 0 0
...........................................................................................................................................GCGAAGGACGTTGGGACG.. 18 2 2 0.50 1 0 1 0
..................................CAAAACGACTAGGCGCCACA......................................................................................................... 20 3 4 0.25 1 0 0 1
......................................................................................................................................CGACGGAGAAGGTCGGTA....... 18 2 9 0.11 1 0 0 1
................................................................................................................AGGACTAGGGCGAAGTCG............................. 18 2 17 0.06 1 0 0 1
.................................................................................................TAGGCGTCGAAGCCGAGGA........................................... 19 3 20 0.05 1 0 0 1

Re-alignment of all predicted orthologs

Species Coordinate ID Type Alignment
droWil2 scf2_1100000004909:7153684-7153842 - dwi_135 candidate CAATTGACATGAAGACAATTTTACTTTTGTGGATGTTTTGCGGATCCGGGTTGTTGTTGTTGCCAGCCTGGGCCGAACAAAATGCCAATGCAACAGCAACAGCAACTTCGGCTCCTGCTCCTGCTTCAGCTGCGGCTGCCGCTTCCAGCAATCCTGCCC
droGri2 scaffold_15203:3522820-3522879 + --------------------------------------------------------------------------------------AATGCAACAACGACAGCCACAGCCGTTTCGGCTGTGTCTGCAGCAGCAGCTGCAGCGTCC-------------
droPer2 scaffold_13:308965-309012 + --------------------------------------------------------------------------------------------------------------GCTCCTGCTGCTGCTGCTGCTGCTGCTGACGCGTTTCGTGGCTGTGCC-

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droWil2
CAATTGACATGAAGACAATTTTACTTTTGTGGATGTTTTGCGGATCCGGGTTGTTGTTGTTGCCAGCCTGGGCCGAACAAAATGCCAATGCAACAGCAACAGCAACTTCGGCTCCTGCTCCTGCTTCAGCTGCGGCTGCCGCTTCCAGCAATCCTGCCCSizeHit CountTotal NormTotalM020

Head		M045

Female-body		V117

Male-body		V118

Embryo		V119

Head
..................................................TTGTTGTTGTTGCCAGCCTGGGC......................................................................................23138.0038000533
..................................................TTGTTGTTGTTGCCAGCCTGGG.......................................................................................22114.0014100211
..................................................TTGTTGTTGTTGCCAGCCTGG........................................................................................2113.00301002
...........................................ATCCGGGTTGTTGTTGTTGCC...............................................................................................2111.00100001
..................................................TTGTTGTTGTTGCCAGCCTG.........................................................................................2011.00101000
..................................................TTGTTGTTGTTGCCAGCCTGGC.......................................................................................2211.00100001
....................................................................................................................GCTCCTGCTTCAGCTGCGGC.......................2011.00101000
.....................................................................................CAATGCAACAGCAACAGCAACTT...................................................2311.00100001
.....................................................TTGTTGTTGCCAGCCTGGGC......................................................................................2011.00100001
....................................................GTTGTTGTTGCCAGCCTGGGC......................................................................................2111.00100010
...........................................................................................AACAGCAACAGCAACTTCGGCTCCTG..........................................2611.00101000
.....................................................................................................GCAACTTCGGCTCCTGCTCCTGCTTCA...............................2711.00101000
..................................GTTTTGCGGATCCGGGTT...........................................................................................................1811.00101000
..................................................TTGTTGTTGTTGCCAGCCTGGGT......................................................................................2311.00100001
..................................................TTGTTGTTGTTGCCAGCCTGGGCCGT...................................................................................2611.00101000
...............................GATGTTTTGCGGATCCGGGTTGT.........................................................................................................2311.00100100
droGri2
--------------------------------------------------------------------------------------AATGCAACAACGACAGCCACAGCCGTTTCGGCTGTGTCTGCAGCAGCAGCTGCAGCGTCC-------------SizeHit CountTotal NormTotalM041

Female-body		V109

Male-body
No Reads
droPer2
--------------------------------------------------------------------------------------------------------------GCTCCTGCTGCTGCTGCTGCTGCTGCTGACGCGTTTCGTGGCTGTGCC-SizeHit CountTotal NormTotalM021

Embryo		M042

Female-body		V042

Embryo		V050

Head		V057

Head		V111

Male-body
No Reads

Generated: 10/20/2015 at 07:44 PM