ID:

dwi_1214

 Coordinate:

scf2_1100000004521:2167348-2167498 +

 Confidence:

Candidate

 Type:

Unknown

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-18.4 -18.4

Flybase annnotation

CDS [Dwil\GK15144-cds]; exon [dwil_GLEANR_15417:6]; intron [Dwil\GK15144-in]

No Repeatable elements found

Sense Strand Reads

##################################################---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
CACTTGCGCAAAATTTTGATTGCGCTGGGTTTATACCGCTAGTTCGACAAGTATATGAGCGGATGTATGTTGAGGATTCCCGTCTCCCTCGCAGCTTACCATTTCCAATATTTCAGCATGCAATGACGGAGTTTTTAACCGCGTATATGATACATCAGGGTAAATTTATCTTAAAGATTCCGGCTTTACAATCTTTAATGGACCCATTAGCAGCCATTAGTGCTGAGAGCTACAATATTCCAAAACCTATA
*****************************************************...............((((((((((((.(((......((((((..................))).)))...))))))))))))))).*************************************************************************************************************** 		Read size # Mismatch Hit Count Total Norm Total V118
embryo
 V117
male body		 M045
female body
 V119
head
 M020
head
.........AAAATTTTGATTGCGCTGGGT............................................................................................................................................................................................................................. 21 0 1 3.00 3 1 2 0 0 0
.....................................................TATGAGCGGATGTATGTTGAGG................................................................................................................................................................................ 22 0 1 1.00 1 0 1 0 0 0
......................................................................................................................TGCAATGACGGAGTTTTTAACC............................................................................................................... 22 0 1 1.00 1 1 0 0 0 0
................................ATACCGCTAGTTCGACAAGT....................................................................................................................................................................................................... 20 0 1 1.00 1 1 0 0 0 0
...............................................................TGTATGTTGAGGATTCCCGT........................................................................................................................................................................ 20 0 1 1.00 1 0 1 0 0 0
...........AATTTTGATTGCGCTGGGT............................................................................................................................................................................................................................. 19 0 1 1.00 1 1 0 0 0 0
...................................................TATATGAGCGGATGTATGTTG................................................................................................................................................................................... 21 0 1 1.00 1 0 0 1 0 0
...............................TATACCGCTAGTTCGACAAGTATATG.................................................................................................................................................................................................. 26 0 1 1.00 1 0 0 1 0 0
.......................................................................................................................................................................................................................ATTAGTGCTGAGAGCTACAATAT............. 23 0 1 1.00 1 0 1 0 0 0
.................GATTGCGCTGGGTTTATACCG..................................................................................................................................................................................................................... 21 0 1 1.00 1 0 0 0 1 0
.........AAAATTTTGATTGCGCTGGGTT............................................................................................................................................................................................................................ 22 0 1 1.00 1 0 1 0 0 0
.......................................TAGTTCGACAAGTATATGAGC............................................................................................................................................................................................... 21 0 1 1.00 1 1 0 0 0 0
..............................................................................................................................................GTATATGATACATCAGGGT.......................................................................................... 19 0 1 1.00 1 1 0 0 0 0
......................................................................................................................................................................TATCTTAAAGATTCCGGCT.................................................................. 19 0 1 1.00 1 0 0 0 0 1
................................................................................................................................................ATATGATACATCAGGGTAAAT...................................................................................... 21 0 1 1.00 1 0 0 1 0 0
...............................................................................................................TTCAGCATGCAATGACGGA......................................................................................................................... 19 0 1 1.00 1 1 0 0 0 0
...........................................................................................................................................................CAGGGTAACTATAACTTAAAG........................................................................... 21 3 2 0.50 1 0 0 0 1 0
...........................................................................................................................................................................TGGGGATTCCGGCTTTACAAT........................................................... 21 3 10 0.10 1 0 0 1 0 0
....................................................ATCTGAGCGGATGTGTGGT.................................................................................................................................................................................... 19 3 20 0.05 1 0 0 1 0 0
..........................................................ACGGATGAATGTTGAAGAT.............................................................................................................................................................................. 19 3 20 0.05 1 0 0 1 0 0

Anti-sense strand reads

GTGAACGCGTTTTAAAACTAACGCGACCCAAATATGGCGATCAAGCTGTTCATATACTCGCCTACATACAACTCCTAAGGGCAGAGGGAGCGTCGAATGGTAAAGGTTATAAAGTCGTACGTTACTGCCTCAAAAATTGGCGCATATACTATGTAGTCCCATTTAAATAGAATTTCTAAGGCCGAAATGTTAGAAATTACCTGGGTAATCGTCGGTAATCACGACTCTCGATGTTATAAGGTTTTGGATAT
***************************************************************************************************************...............((((((((((((.(((......((((((..................))).)))...))))))))))))))).***************************************************** 		Read size # Mismatch Hit Count Total Norm Total M045
female body
 V117
male body
.....................................................................................................................................................TATGTGGTCCCAGTTAAATAGCAT.............................................................................. 24 3 1 1.00 1 1 0
.........................................................................CCTAAGGGGAGAGGGCGCGT.............................................................................................................................................................. 20 2 1 1.00 1 1 0
.........................................................................................................................................................................................................TGGGTAATCGTCGGTAATCAC............................. 21 0 1 1.00 1 1 0
..........................................................................................CGTTGCATCGTAAAGGTTATAA........................................................................................................................................... 22 3 1 1.00 1 0 1
.........................................................................CCTAGGGGCAGATGGAGTGT.............................................................................................................................................................. 20 3 5 0.20 1 1 0
...........................................................................................................................................................GTCCCAGTTAAATAGCAT.............................................................................. 18 2 8 0.13 1 1 0

Re-alignment of all predicted orthologs

Species Coordinate ID Alignment
droWil2 scf2_1100000004521:2167298-2167548 + dwi_1214 CACT--TGCGCAAAATTTTGATTGCGCTGGGTTTATACCGCTAGTTCGACAAGTATATGAGCGGATGTATGTTGAGGATTCCCGTCTCCCTCGCAGCTTACCATTTCCAATATTTCAGCATGCAATGACGGAGTTTTTAACCGCGTATATGATACATCAGGGTAAATTTATCTTAAAGATTCCGGCTTTACAATCTTTAATGGACCCATTAGCAGCCATTAGTGCTGAGAGCTACAATATTCCAAAACCTATA
droKik1 scf7180000302270:261881-262096 + CT----TGGCCAAAACTTTGACTTTGCTGGCTTTATTCCGCTTGTAAAATCAGTATATATATATATGGCGGCGAGGGACTACCACCTCGAGAGACGGTTGCCCTTCTGTGTCTTTCAACATGCCATGACTGAAATCCTTACGGCAAGAATGTTGGAAGTGGCGCGG------C--------------------------ATCG-TTGTTATGCAAAATACTATTACGAAGATTTCTTTATACCACTGCCGATC
droEle1 scf7180000491024:259447-259642 - CATA--AGGCCAGAATTTTGA------------------------------------------------------GGATCAAAAGATGACATGAAGCCTCCCTTTTCCAATTTTCCAGCACGCGATGACGGAATTTTTAGTTGCCTACCAATTGTATCAAGGAAATTACATCCTCAATATACCTGAGCTCCAGAGAATGATGGATTCAC-AGTAGCAATTAGTGCTAGTGAGTATAATATTCCACAGCCAATA
droTak1 scf7180000414050:285745-285982 + CATTTGTGGTCAGAACTTTGACTTTGCGGGCTTTATTCCGCTAGTCCGAGAAGTTTACTTTATTATGGAGAGGGAGGACTATCTTCTACCAAGGAGGTTACCCATCTGCGTGTTTCAGCATGCGATGACTGAAGCCCTGACGGCCCACATGATTCAAGAGGCAAGG------TTCGAAGTTCCTCGCCTC-------CAATCGATTGGTATGCATGTTCCATTTTCTTATATGACA--ATCCCGTATCCGATC

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droWil2
CACT--TGCGCAAAATTTTGATTGCGCTGGGTTTATACCGCTAGTTCGACAAGTATATGAGCGGATGTATGTTGAGGATTCCCGTCTCCCTCGCAGCTTACCATTTCCAATATTTCAGCATGCAATGACGGAGTTTTTAACCGCGTATATGATACATCAGGGTAAATTTATCTTAAAGATTCCGGCTTTACAATCTTTAATGGACCCATTAGCAGCCATTAGTGCTGAGAGCTACAATATTCCAAAACCTATASizeHit CountTotal NormTotalM020
Head		M045
Female-body		V117
Male-body		V118
Embryo		V119
Head
...........AAAATTTTGATTGCGCTGGGT.............................................................................................................................................................................................................................2113.00300210
.......................................................TATGAGCGGATGTATGTTGAGG................................................................................................................................................................................2211.00100100
........................................................................................................................TGCAATGACGGAGTTTTTAACC...............................................................................................................2211.00100010
..................................ATACCGCTAGTTCGACAAGT.......................................................................................................................................................................................................2011.00100010
.................................................................TGTATGTTGAGGATTCCCGT........................................................................................................................................................................2011.00100100
.............AATTTTGATTGCGCTGGGT.............................................................................................................................................................................................................................1911.00100010
.....................................................TATATGAGCGGATGTATGTTG...................................................................................................................................................................................2111.00101000
.................................TATACCGCTAGTTCGACAAGTATATG..................................................................................................................................................................................................2611.00101000
.........................................................................................................................................................................................................................ATTAGTGCTGAGAGCTACAATAT.............2311.00100100
...................GATTGCGCTGGGTTTATACCG.....................................................................................................................................................................................................................2111.00100001
...........AAAATTTTGATTGCGCTGGGTT............................................................................................................................................................................................................................2211.00100100
.........................................TAGTTCGACAAGTATATGAGC...............................................................................................................................................................................................2111.00100010
................................................................................................................................................GTATATGATACATCAGGGT..........................................................................................1911.00100010
........................................................................................................................................................................TATCTTAAAGATTCCGGCT..................................................................1911.00110000
..................................................................................................................................................ATATGATACATCAGGGTAAAT......................................................................................2111.00101000
.................................................................................................................TTCAGCATGCAATGACGGA.........................................................................................................................1911.00100010
droKik1
CT----TGGCCAAAACTTTGACTTTGCTGGCTTTATTCCGCTTGTAAAATCAGTATATATATATATGGCGGCGAGGGACTACCACCTCGAGAGACGGTTGCCCTTCTGTGTCTTTCAACATGCCATGACTGAAATCCTTACGGCAAGAATGTTGGAAGTGGCGCGG------C--------------------------ATCG-TTGTTATGCAAAATACTATTACGAAGATTTCTTTATACCACTGCCGATCSizeHit CountTotal NormTotal
No Reads
droEle1
CATA--AGGCCAGAATTTTGA------------------------------------------------------GGATCAAAAGATGACATGAAGCCTCCCTTTTCCAATTTTCCAGCACGCGATGACGGAATTTTTAGTTGCCTACCAATTGTATCAAGGAAATTACATCCTCAATATACCTGAGCTCCAGAGAATGATGGATTCAC-AGTAGCAATTAGTGCTAGTGAGTATAATATTCCACAGCCAATASizeHit CountTotal NormTotal
No Reads
droTak1
CATTTGTGGTCAGAACTTTGACTTTGCGGGCTTTATTCCGCTAGTCCGAGAAGTTTACTTTATTATGGAGAGGGAGGACTATCTTCTACCAAGGAGGTTACCCATCTGCGTGTTTCAGCATGCGATGACTGAAGCCCTGACGGCCCACATGATTCAAGAGGCAAGG------TTCGAAGTTCCTCGCCTC-------CAATCGATTGGTATGCATGTTCCATTTTCTTATATGACA--ATCCCGTATCCGATCSizeHit CountTotal NormTotal
No Reads

Generated: 05/18/2015 at 12:55 AM