dwi_1192

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------################################################## ATACTAGTAATTAAAATAATTGTTTTCTTGCAGGTCATCAAATCGATCCACAGCGTTTTTGAAGACGGCAAGTCTATTACAGCAGCGCTTTTAGCGGGTGTATTTTTTCTAATTGGAAAAAGGTAAGTAGCAAGTGTTAGTTTTACAATAGGCAAGCATATTGGGTATAGACTAATTGTATGTTATTGTTTTCTTTTTCAGAAACAGCACCATAACAGGACCCAGGACAAGGATTGCATGCATCTTGACCC **********************************************************************************************************.....................(((...((((((((..(((((........))))).....))))))))....)))******************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V118 embryo	M045 female body	M020 head	V119 head	V117 male body
..............................................................................................................................................................TATTGGGTATAGACTAATTGTATGT....................................................................	25	0	1	6.00	6	6	0	0	0	0
....................................................................................................................................................................................................................TAACAGGACCCAGGACAAGGATTGCA.............	26	0	2	4.50	9	3	3	3	0	0
....................................................................................................................................................................................................TTCAGAAACAGCACCATAACAGGACC.............................	26	0	1	3.00	3	1	2	0	0	0
..................................TCATCAAATCGATCCACAGCGTTTT................................................................................................................................................................................................	25	0	2	3.00	6	5	1	0	0	0
...............................................................GACGGCAAGCCTGTTACGGC........................................................................................................................................................................	20	3	1	2.00	2	0	2	0	0	0
............................................................................................................CTAATTGGAAAAAGGTAAGTAGCAAG.....................................................................................................................	26	0	1	2.00	2	0	2	0	0	0
...............................................................................................................................TAGCAAGTGTTAGTTTTACAATAGGCA.................................................................................................	27	0	1	2.00	2	2	0	0	0	0
..............................................................................................................................................................................................................................CAGGACAAGGATTGCATGCATCTTGA...	26	0	2	1.50	3	1	2	0	0	0
....................................................................................................................................................................................................................TAACAGGACCCAGGACAAGGATTGC..............	25	0	2	1.50	3	3	0	0	0	0
.................................................................................................................................................................TGGGTATAGACTAATTGTATGTTAT.................................................................	25	0	2	1.50	3	2	0	0	1	0
..................................................................................................................................................................................TATGTTATTGTGTTCTTTTTCAGAAAC..............................................	27	1	1	1.00	1	0	1	0	0	0
...........................................................................................TAGCGGGTGTATTTTTTCTAATTGGAA.....................................................................................................................................	27	0	1	1.00	1	1	0	0	0	0
..............................................................................................................................................................TATTGGGTATAGACTAATTGTATGTT...................................................................	26	0	1	1.00	1	1	0	0	0	0
...........................................CGATCCACAGCGTTTTTGAAGACGG.......................................................................................................................................................................................	25	0	2	1.00	2	2	0	0	0	0
.................................................................................................................................................................TGGGTATAGACTAATTGTATGTTATTG...............................................................	27	0	2	1.00	2	2	0	0	0	0
....................................................................................................................................................................................................TTCAGAAACAGCACCATAACAGGACCCAG..........................	29	0	1	1.00	1	0	1	0	0	0
..................................................................................................................................CAAGTGTTAGTTTTACAATAGGCAAGCA.............................................................................................	28	0	1	1.00	1	0	1	0	0	0
...........................................................................................................................TAAGTAGCAAGTGTTAGTTTTACAATG.....................................................................................................	27	1	1	1.00	1	1	0	0	0	0
...........................................................................................................................TAAGTAGCAAGTGTTAGTTTTACAATA.....................................................................................................	27	0	1	1.00	1	1	0	0	0	0
.............................................................................................................................................................................................................AGCACCATAACAGGACCCAGGACAAG....................	26	0	2	1.00	2	1	1	0	0	0
....................TGTTTTCTTGCAGGTCATCAAATCGA.............................................................................................................................................................................................................	26	0	2	1.00	2	1	1	0	0	0
...............................................................................................................................................................................................TCTTTTTCAGAAACAGCACCATAACAG.................................	27	0	1	1.00	1	1	0	0	0	0
....................................................................................................................................................................................................TTCAGAAACAGGACCATAACAGGACC.............................	26	1	1	1.00	1	0	1	0	0	0
.........................................................................................................................................TAGTTTTACAATAGGCAAGCATAT..........................................................................................	24	0	1	1.00	1	0	1	0	0	0
..............................................................................................................................GTAGCAAGTGATAGTGTT...........................................................................................................	18	2	8	1.00	8	6	0	0	2	0
............................TGCAGGTCATCAAATCGATCCACAGCG....................................................................................................................................................................................................	27	0	2	1.00	2	0	2	0	0	0
..........................................................................................................................................................AGCATATTGGGTATAGACTAATTG.........................................................................	24	0	1	1.00	1	1	0	0	0	0
...........................................................TGAAGACGGCAAGTCTATTACAGCAGC.....................................................................................................................................................................	27	0	1	1.00	1	0	1	0	0	0
...............................................................................................................................TAGCAAGTGTTAGTTTTACAATAGGC..................................................................................................	26	0	1	1.00	1	0	1	0	0	0
.........................................................................CTATTACAGCAGCGCTTTTAGCGGG.........................................................................................................................................................	25	0	1	1.00	1	0	1	0	0	0
......................................................................AGTCTATTACAGCAGCGCTTTTAG.............................................................................................................................................................	24	0	1	1.00	1	1	0	0	0	0
.................................................................................................................................................................................................................................GACAAGGATTGCATGCATCTTGACCC	26	0	2	1.00	2	0	2	0	0	0
......................................................................AGTCTATTACAGCAGCGCTTTTAGCGGGA........................................................................................................................................................	29	1	1	1.00	1	1	0	0	0	0
.........................TCTTGCAGGTCATCAAATCGATCCACA.......................................................................................................................................................................................................	27	0	2	1.00	2	0	2	0	0	0
..........................................................................TATTACAGCAGCGCTTTTAGCGGGT........................................................................................................................................................	25	0	1	1.00	1	1	0	0	0	0
........................................................................................TTTTAGCGGGTGTATTTTTTCTAATTG........................................................................................................................................	27	0	1	1.00	1	1	0	0	0	0
........................................................TTTTGAAGACGGCAAGTCTATTACAGC........................................................................................................................................................................	27	0	1	1.00	1	0	1	0	0	0
..............................................................................................................................................TTACAATAGGCAAGCATATTGGGTA....................................................................................	25	0	1	1.00	1	0	1	0	0	0
....................................................................................................................................................................................................TTCAGAAACAGCACCATAACAGGACCCA...........................	28	0	1	1.00	1	0	1	0	0	0
............................................................................................................................................................................TAATTGTATGTTATTGTTTTCTTT.......................................................	24	0	2	1.00	2	0	2	0	0	0
..........................................................................TATTACAGCAGCGCTTTTAGCGGGTG.......................................................................................................................................................	26	0	1	1.00	1	0	1	0	0	0
...........................................................................................................................................................................................................ACAGCACCATAACAGGACCCAGGAC.......................	25	0	2	0.50	1	1	0	0	0	0
............................................................................................................................................................................TAATTGTATGTTATTGTTTTCTT........................................................	23	0	2	0.50	1	1	0	0	0	0
...................................CATCAAATCGATCCACAGCGTTTT................................................................................................................................................................................................	24	0	2	0.50	1	1	0	0	0	0
.........................................ATCGATCCACAGCGTTTTTGAAGAC.........................................................................................................................................................................................	25	0	2	0.50	1	1	0	0	0	0
............................TGCAGGTCATCAAATCGATCCACAGCGT...................................................................................................................................................................................................	28	0	2	0.50	1	1	0	0	0	0
....................................................................................................................................................................................................................TAACAGGACCCAGGACAAGGAT.................	22	0	2	0.50	1	1	0	0	0	0
........................................................TTTTGAAGACGGCAAGTCTATTACA..........................................................................................................................................................................	25	0	2	0.50	1	0	1	0	0	0
....................................................................................................................................................................................................................TAACAGGACCCAGGACAAGGATTGT..............	25	1	2	0.50	1	0	1	0	0	0
..................................................................................................................................................................................................................CATAACAGGACCCAGGACAAGGATTGCA.............	28	0	2	0.50	1	0	1	0	0	0
.....................................................................................................................................................................................................................AACAGGACCCAGGACAAGGATTGCA.............	25	0	2	0.50	1	1	0	0	0	0
..................................................................................................................................................................................................................CATAACAGGACCCAGGACAAGGATTG...............	26	0	2	0.50	1	1	0	0	0	0
..............................................TCCACAGCGTTTTTGAAGACGGCAAG...................................................................................................................................................................................	26	0	2	0.50	1	0	1	0	0	0
............................................................................................................................................................................................................................CCCAGGACAAGGATTGCATGCATCT......	25	0	2	0.50	1	1	0	0	0	0
....................................................................................................................................................................GTATAGACTAATTGTATGTTATTGTT.............................................................	26	0	2	0.50	1	0	1	0	0	0
.............................................................................................................................................................................................................................CCAGGACAAGGATTGCATGCATCTTGA...	27	0	2	0.50	1	1	0	0	0	0
....................................................................................................................................................................................................................TAACAGGACCCAGGACAAGGATTG...............	24	0	2	0.50	1	0	1	0	0	0
.....................................................................................................................................................................TATAGACTAATTGTATGTTATTGT..............................................................	24	0	2	0.50	1	1	0	0	0	0
.........................TCTTGCAGGTCATCAAATCGATCCAC........................................................................................................................................................................................................	26	0	2	0.50	1	0	1	0	0	0
.........ATTAAAATAATTGTTTTCTTGCAGG.........................................................................................................................................................................................................................	25	0	2	0.50	1	0	1	0	0	0
.................................................................................................................................................................TGGGTATAGACTAATTGTATGT....................................................................	22	0	2	0.50	1	1	0	0	0	0
................................................................................................................................................................TTGGGTATAGACTAATTGTATGTTA..................................................................	25	0	2	0.50	1	0	1	0	0	0
.....................................................................................................................................................................TATAGACTAATTGTATGTTATTGTTT............................................................	26	0	2	0.50	1	0	1	0	0	0
........................TTCTTGCAGGTCATCAAATCGATCCACA.......................................................................................................................................................................................................	28	0	2	0.50	1	0	1	0	0	0
................................................................................................................................................................TTGGGTATAGACTAATTGTATGTTAT.................................................................	26	0	2	0.50	1	1	0	0	0	0
....................TGTTTTCTTGCAGGTCATCAAATCGAT............................................................................................................................................................................................................	27	0	2	0.50	1	1	0	0	0	0
..................................................................................................................................................................................................................CATAACAGGACCCAGGACAAGGATTGC..............	27	0	2	0.50	1	1	0	0	0	0
............................................................................................................................................................................................................CAGCACCATAACAGGACCCAGGACAA.....................	26	0	2	0.50	1	1	0	0	0	0
........................................AATCGATCCACAGCGTTTTTGAAGACGG.......................................................................................................................................................................................	28	0	2	0.50	1	0	1	0	0	0
..........................................TCGATCCACAGCGTTTTTGAAGACGG.......................................................................................................................................................................................	26	0	2	0.50	1	1	0	0	0	0
.......................................AAATCGATCCACAGCGTTTTTGAAGA..........................................................................................................................................................................................	26	0	2	0.50	1	1	0	0	0	0
..................................................................................................................................................................................................................CATAACAGGACCCAGGACAAGGAT.................	24	0	2	0.50	1	0	1	0	0	0
.............................................................................................................................................................................................................AGCACCATAACAGGACCCAGGACAA.....................	25	0	2	0.50	1	0	1	0	0	0
............................................................................................................................................................................TAATTGTATGTTATTGTTTTCTTTTT.....................................................	26	0	2	0.50	1	0	1	0	0	0
..........................................................................................................................................................................................................................GACCCAGGACAAGGATTGCATGCATC.......	26	0	2	0.50	1	0	0	0	1	0
..............................CAGGTCATCAAATCGATCCACAGCGTT..................................................................................................................................................................................................	27	0	2	0.50	1	0	1	0	0	0
..............................................................................................................................................................................................................................CAGGACAAGGATTGCATGCATCTT.....	24	0	2	0.50	1	1	0	0	0	0
.....................................TCAAATCGATCCACAGCGTTTTTGA.............................................................................................................................................................................................	25	0	2	0.50	1	1	0	0	0	0
.................................................................................................................................................................TGGGTATAGACTAATTGTATGTTATT................................................................	26	0	2	0.50	1	1	0	0	0	0
..............................................TCCACAGCGTTTTTGAAGACGGCAAGTC.................................................................................................................................................................................	28	0	2	0.50	1	0	1	0	0	0
.....................................TCAAATCGATCCACAGCGTTTTTGAAG...........................................................................................................................................................................................	27	0	2	0.50	1	1	0	0	0	0
...............................................................GACGGCAAGCCTGTTACGG.........................................................................................................................................................................	19	3	2	0.50	1	0	1	0	0	0
.......................TTTCTTGCAGGTCATCAAATCGATC...........................................................................................................................................................................................................	25	0	2	0.50	1	0	1	0	0	0
.............................................................................................................................................................................................................AGCACCATAACAGGACCCAGGACAAGGA..................	28	0	2	0.50	1	0	1	0	0	0
........................................................................................TTTTAGCGGGAGTATCTTTAC..............................................................................................................................................	21	3	6	0.33	2	1	0	0	0	1
.........................................................................................TTTAGCGGGAGTATCTTTAC..............................................................................................................................................	20	3	8	0.13	1	0	0	0	0	1
................................................................................................................................................................................................................ACCATTACAGGACACAGCAC.......................	20	3	8	0.13	1	0	1	0	0	0
..................ATAGTTTTCATGCAGGTC.......................................................................................................................................................................................................................	18	2	11	0.09	1	0	1	0	0	0
...........................................................TGAAGACGGCAAGATTGTT.............................................................................................................................................................................	19	3	20	0.05	1	0	1	0	0	0

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------##################################################
ATACTAGTAATTAAAATAATTGTTTTCTTGCAGGTCATCAAATCGATCCACAGCGTTTTTGAAGACGGCAAGTCTATTACAGCAGCGCTTTTAGCGGGTGTATTTTTTCTAATTGGAAAAAGGTAAGTAGCAAGTGTTAGTTTTACAATAGGCAAGCATATTGGGTATAGACTAATTGTATGTTATTGTTTTCTTTTTCAGAAACAGCACCATAACAGGACCCAGGACAAGGATTGCATGCATCTTGACCC
************************************************************************************************************.....................(((...((((((((..(((((........))))).....))))))))....)))********************************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

V118
embryo

M045
female body

M020
head

V119
head

V117
male body

..............................................................................................................................................................TATTGGGTATAGACTAATTGTATGT....................................................................

6.00

....................................................................................................................................................................................................................TAACAGGACCCAGGACAAGGATTGCA.............

4.50

....................................................................................................................................................................................................TTCAGAAACAGCACCATAACAGGACC.............................

3.00

..................................TCATCAAATCGATCCACAGCGTTTT................................................................................................................................................................................................

3.00

...............................................................GACGGCAAGCCTGTTACGGC........................................................................................................................................................................

2.00

............................................................................................................CTAATTGGAAAAAGGTAAGTAGCAAG.....................................................................................................................

2.00

...............................................................................................................................TAGCAAGTGTTAGTTTTACAATAGGCA.................................................................................................

2.00

..............................................................................................................................................................................................................................CAGGACAAGGATTGCATGCATCTTGA...

1.50

....................................................................................................................................................................................................................TAACAGGACCCAGGACAAGGATTGC..............

1.50

.................................................................................................................................................................TGGGTATAGACTAATTGTATGTTAT.................................................................

1.50

..................................................................................................................................................................................TATGTTATTGTGTTCTTTTTCAGAAAC..............................................

1.00

...........................................................................................TAGCGGGTGTATTTTTTCTAATTGGAA.....................................................................................................................................

1.00

..............................................................................................................................................................TATTGGGTATAGACTAATTGTATGTT...................................................................

1.00

...........................................CGATCCACAGCGTTTTTGAAGACGG.......................................................................................................................................................................................

1.00

.................................................................................................................................................................TGGGTATAGACTAATTGTATGTTATTG...............................................................

1.00

....................................................................................................................................................................................................TTCAGAAACAGCACCATAACAGGACCCAG..........................

1.00

..................................................................................................................................CAAGTGTTAGTTTTACAATAGGCAAGCA.............................................................................................

1.00

...........................................................................................................................TAAGTAGCAAGTGTTAGTTTTACAATG.....................................................................................................

1.00

...........................................................................................................................TAAGTAGCAAGTGTTAGTTTTACAATA.....................................................................................................

1.00

.............................................................................................................................................................................................................AGCACCATAACAGGACCCAGGACAAG....................

1.00

....................TGTTTTCTTGCAGGTCATCAAATCGA.............................................................................................................................................................................................................

1.00

...............................................................................................................................................................................................TCTTTTTCAGAAACAGCACCATAACAG.................................

1.00

....................................................................................................................................................................................................TTCAGAAACAGGACCATAACAGGACC.............................

1.00

.........................................................................................................................................TAGTTTTACAATAGGCAAGCATAT..........................................................................................

1.00

..............................................................................................................................GTAGCAAGTGATAGTGTT...........................................................................................................

1.00

............................TGCAGGTCATCAAATCGATCCACAGCG....................................................................................................................................................................................................

1.00

..........................................................................................................................................................AGCATATTGGGTATAGACTAATTG.........................................................................

1.00

...........................................................TGAAGACGGCAAGTCTATTACAGCAGC.....................................................................................................................................................................

1.00

...............................................................................................................................TAGCAAGTGTTAGTTTTACAATAGGC..................................................................................................

1.00

.........................................................................CTATTACAGCAGCGCTTTTAGCGGG.........................................................................................................................................................

1.00

......................................................................AGTCTATTACAGCAGCGCTTTTAG.............................................................................................................................................................

1.00

.................................................................................................................................................................................................................................GACAAGGATTGCATGCATCTTGACCC

1.00

......................................................................AGTCTATTACAGCAGCGCTTTTAGCGGGA........................................................................................................................................................

1.00

.........................TCTTGCAGGTCATCAAATCGATCCACA.......................................................................................................................................................................................................

1.00

..........................................................................TATTACAGCAGCGCTTTTAGCGGGT........................................................................................................................................................

1.00

........................................................................................TTTTAGCGGGTGTATTTTTTCTAATTG........................................................................................................................................

1.00

........................................................TTTTGAAGACGGCAAGTCTATTACAGC........................................................................................................................................................................

1.00

..............................................................................................................................................TTACAATAGGCAAGCATATTGGGTA....................................................................................

1.00

....................................................................................................................................................................................................TTCAGAAACAGCACCATAACAGGACCCA...........................

1.00

............................................................................................................................................................................TAATTGTATGTTATTGTTTTCTTT.......................................................

1.00

..........................................................................TATTACAGCAGCGCTTTTAGCGGGTG.......................................................................................................................................................

1.00

...........................................................................................................................................................................................................ACAGCACCATAACAGGACCCAGGAC.......................

0.50

............................................................................................................................................................................TAATTGTATGTTATTGTTTTCTT........................................................

0.50

...................................CATCAAATCGATCCACAGCGTTTT................................................................................................................................................................................................

0.50

.........................................ATCGATCCACAGCGTTTTTGAAGAC.........................................................................................................................................................................................

0.50

............................TGCAGGTCATCAAATCGATCCACAGCGT...................................................................................................................................................................................................

0.50

....................................................................................................................................................................................................................TAACAGGACCCAGGACAAGGAT.................

0.50

........................................................TTTTGAAGACGGCAAGTCTATTACA..........................................................................................................................................................................

0.50

....................................................................................................................................................................................................................TAACAGGACCCAGGACAAGGATTGT..............

0.50

..................................................................................................................................................................................................................CATAACAGGACCCAGGACAAGGATTGCA.............

0.50

.....................................................................................................................................................................................................................AACAGGACCCAGGACAAGGATTGCA.............

0.50

..................................................................................................................................................................................................................CATAACAGGACCCAGGACAAGGATTG...............

0.50

..............................................TCCACAGCGTTTTTGAAGACGGCAAG...................................................................................................................................................................................

0.50

............................................................................................................................................................................................................................CCCAGGACAAGGATTGCATGCATCT......

0.50

....................................................................................................................................................................GTATAGACTAATTGTATGTTATTGTT.............................................................

0.50

.............................................................................................................................................................................................................................CCAGGACAAGGATTGCATGCATCTTGA...

0.50

....................................................................................................................................................................................................................TAACAGGACCCAGGACAAGGATTG...............

0.50

.....................................................................................................................................................................TATAGACTAATTGTATGTTATTGT..............................................................

0.50

.........................TCTTGCAGGTCATCAAATCGATCCAC........................................................................................................................................................................................................

0.50

.........ATTAAAATAATTGTTTTCTTGCAGG.........................................................................................................................................................................................................................

0.50

.................................................................................................................................................................TGGGTATAGACTAATTGTATGT....................................................................

0.50

................................................................................................................................................................TTGGGTATAGACTAATTGTATGTTA..................................................................

0.50

.....................................................................................................................................................................TATAGACTAATTGTATGTTATTGTTT............................................................

0.50

........................TTCTTGCAGGTCATCAAATCGATCCACA.......................................................................................................................................................................................................

0.50

................................................................................................................................................................TTGGGTATAGACTAATTGTATGTTAT.................................................................

0.50

....................TGTTTTCTTGCAGGTCATCAAATCGAT............................................................................................................................................................................................................

0.50

..................................................................................................................................................................................................................CATAACAGGACCCAGGACAAGGATTGC..............

0.50

............................................................................................................................................................................................................CAGCACCATAACAGGACCCAGGACAA.....................

0.50

........................................AATCGATCCACAGCGTTTTTGAAGACGG.......................................................................................................................................................................................

0.50

..........................................TCGATCCACAGCGTTTTTGAAGACGG.......................................................................................................................................................................................

0.50

.......................................AAATCGATCCACAGCGTTTTTGAAGA..........................................................................................................................................................................................

0.50

..................................................................................................................................................................................................................CATAACAGGACCCAGGACAAGGAT.................

0.50

.............................................................................................................................................................................................................AGCACCATAACAGGACCCAGGACAA.....................

0.50

............................................................................................................................................................................TAATTGTATGTTATTGTTTTCTTTTT.....................................................

0.50

..........................................................................................................................................................................................................................GACCCAGGACAAGGATTGCATGCATC.......

0.50

..............................CAGGTCATCAAATCGATCCACAGCGTT..................................................................................................................................................................................................

0.50

..............................................................................................................................................................................................................................CAGGACAAGGATTGCATGCATCTT.....

0.50

.....................................TCAAATCGATCCACAGCGTTTTTGA.............................................................................................................................................................................................

0.50

.................................................................................................................................................................TGGGTATAGACTAATTGTATGTTATT................................................................

0.50

..............................................TCCACAGCGTTTTTGAAGACGGCAAGTC.................................................................................................................................................................................

0.50

.....................................TCAAATCGATCCACAGCGTTTTTGAAG...........................................................................................................................................................................................

0.50

...............................................................GACGGCAAGCCTGTTACGG.........................................................................................................................................................................

0.50

.......................TTTCTTGCAGGTCATCAAATCGATC...........................................................................................................................................................................................................

0.50

.............................................................................................................................................................................................................AGCACCATAACAGGACCCAGGACAAGGA..................

0.50

........................................................................................TTTTAGCGGGAGTATCTTTAC..............................................................................................................................................

0.33

.........................................................................................TTTAGCGGGAGTATCTTTAC..............................................................................................................................................

0.13

................................................................................................................................................................................................................ACCATTACAGGACACAGCAC.......................

0.13

..................ATAGTTTTCATGCAGGTC.......................................................................................................................................................................................................................

0.09

...........................................................TGAAGACGGCAAGATTGTT.............................................................................................................................................................................

0.05

TATGATCATTAATTTTATTAACAAAAGAACGTCCAGTAGTTTAGCTAGGTGTCGCAAAAACTTCTGCCGTTCAGATAATGTCGTCGCGAAAATCGCCCACATAAAAAAGATTAACCTTTTTCCATTCATCGTTCACAATCAAAATGTTATCCGTTCGTATAACCCATATCTGATTAACATACAATAACAAAAGAAAAAGTCTTTGTCGTGGTATTGTCCTGGGTCCTGTTCCTAACGTACGTAGAACTGGG ******************************************************************.....................(((...((((((((..(((((........))))).....))))))))....)))**********************************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M045 female body	V118 embryo	M020 head
...................................................................................................................................................TATCCGTTCGTATAACCCATATCT................................................................................	24	0	1	3.00	3	1	0	2
........................................................................................................................TCCATTCATCGTTCACAATCAAAATGT........................................................................................................	27	0	1	1.00	1	1	0	0
........................AAGAACGTCCAGTAGTTTAGCTA............................................................................................................................................................................................................	23	0	2	1.00	2	2	0	0
...................................................................................................................................................................................................ATAGTCTTTGTCGTGGTTTTGTCCTG..............................	26	2	1	1.00	1	1	0	0
.........................................................................................................................CCATTCATCGTTCACAATCAAAATG.........................................................................................................	25	0	1	1.00	1	1	0	0
......................................................................TCAGATAATGTCGTCGCGAA.................................................................................................................................................................	20	0	1	1.00	1	1	0	0
...........................................GCTAGGTGTCGCAAAAACTTCTGCCGT.....................................................................................................................................................................................	27	0	2	0.50	1	1	0	0
.....................CAAAAGAACGTCCAGTAGTTTAGCTA............................................................................................................................................................................................................	26	0	2	0.50	1	1	0	0
.....................................AGTTTAGCTAGGTGTCGCAAAAACT.............................................................................................................................................................................................	25	0	2	0.50	1	0	1	0
...............................TCCAGTAGTTTAGCTAGGTGTCGCAAA.................................................................................................................................................................................................	27	0	2	0.50	1	0	1	0
............................................CTAGGTGTCGCAAAAACTTCTGCCG......................................................................................................................................................................................	25	0	2	0.50	1	1	0	0
.......................AAAGAACGTCCAGTAGTTTAGCTA............................................................................................................................................................................................................	24	0	2	0.50	1	1	0	0
.........................AGAACGTCCAGTAGTTTAGCTA............................................................................................................................................................................................................	22	0	2	0.50	1	0	1	0
....................ACAAAAGAACGTCCAGTAGTTTAGCTA............................................................................................................................................................................................................	27	0	2	0.50	1	1	0	0
...........................................................................................................................................................................................................................TGGGTCCTGTTCCTAACGTACGTAGA......	26	0	2	0.50	1	0	1	0
.......................................................................................................................................................................................................................GTCCTGGGTCCTGTTCCTAACGTACGT.........	27	0	2	0.50	1	1	0	0
.............................................................................................................................................................................................................................GGTCCTGTTCCTAACGTACGTAG.......	23	0	2	0.50	1	1	0	0
.......................................................................................................................................................................................................................GTCCTGGGTCCTGTTCCTAACG..............	22	0	2	0.50	1	1	0	0
..................................................GTCGCAAAAACTTCTGCCGTTCAGAT...............................................................................................................................................................................	26	0	2	0.50	1	1	0	0
............TTTTATTAACAAAAGAACGTCCAGTA.....................................................................................................................................................................................................................	26	0	2	0.50	1	1	0	0
............................................................................................................................................................................................................................GGGTCCTGTTCCTAACGTACGTAG.......	24	0	2	0.50	1	1	0	0
....................................................................................................................................................................................................AAGTCTTTGTTGTGGTCTT....................................	19	2	15	0.07	1	0	1	0
............................................................................................................................................................................................AAAAGAAAATGTCTTTGTCA...........................................	20	2	17	0.06	1	1	0	0

TATGATCATTAATTTTATTAACAAAAGAACGTCCAGTAGTTTAGCTAGGTGTCGCAAAAACTTCTGCCGTTCAGATAATGTCGTCGCGAAAATCGCCCACATAAAAAAGATTAACCTTTTTCCATTCATCGTTCACAATCAAAATGTTATCCGTTCGTATAACCCATATCTGATTAACATACAATAACAAAAGAAAAAGTCTTTGTCGTGGTATTGTCCTGGGTCCTGTTCCTAACGTACGTAGAACTGGG
********************************************************************.....................(((...((((((((..(((((........))))).....))))))))....)))************************************************************************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M045
female body

V118
embryo

M020
head

...................................................................................................................................................TATCCGTTCGTATAACCCATATCT................................................................................

3.00

........................................................................................................................TCCATTCATCGTTCACAATCAAAATGT........................................................................................................

1.00

........................AAGAACGTCCAGTAGTTTAGCTA............................................................................................................................................................................................................

1.00

...................................................................................................................................................................................................ATAGTCTTTGTCGTGGTTTTGTCCTG..............................

1.00

.........................................................................................................................CCATTCATCGTTCACAATCAAAATG.........................................................................................................

1.00

......................................................................TCAGATAATGTCGTCGCGAA.................................................................................................................................................................

1.00

...........................................GCTAGGTGTCGCAAAAACTTCTGCCGT.....................................................................................................................................................................................

0.50

.....................CAAAAGAACGTCCAGTAGTTTAGCTA............................................................................................................................................................................................................

0.50

.....................................AGTTTAGCTAGGTGTCGCAAAAACT.............................................................................................................................................................................................

0.50

...............................TCCAGTAGTTTAGCTAGGTGTCGCAAA.................................................................................................................................................................................................

0.50

............................................CTAGGTGTCGCAAAAACTTCTGCCG......................................................................................................................................................................................

0.50

.......................AAAGAACGTCCAGTAGTTTAGCTA............................................................................................................................................................................................................

0.50

.........................AGAACGTCCAGTAGTTTAGCTA............................................................................................................................................................................................................

0.50

....................ACAAAAGAACGTCCAGTAGTTTAGCTA............................................................................................................................................................................................................

0.50

...........................................................................................................................................................................................................................TGGGTCCTGTTCCTAACGTACGTAGA......

0.50

.......................................................................................................................................................................................................................GTCCTGGGTCCTGTTCCTAACGTACGT.........

0.50

.............................................................................................................................................................................................................................GGTCCTGTTCCTAACGTACGTAG.......

0.50

.......................................................................................................................................................................................................................GTCCTGGGTCCTGTTCCTAACG..............

0.50

..................................................GTCGCAAAAACTTCTGCCGTTCAGAT...............................................................................................................................................................................

0.50

............TTTTATTAACAAAAGAACGTCCAGTA.....................................................................................................................................................................................................................

0.50

............................................................................................................................................................................................................................GGGTCCTGTTCCTAACGTACGTAG.......

0.50

....................................................................................................................................................................................................AAGTCTTTGTTGTGGTCTT....................................

0.07

............................................................................................................................................................................................AAAAGAAAATGTCTTTGTCA...........................................

0.06

Species	Coordinate	ID	Alignment
droWil2	scf2_1100000004519:68364-68614 -	dwi_1192	ATACTAGTAATTAAAATAATTGTTTTCTTGCAGGTCATCAAATCGATCCACAGCGTTTTTGAAGACGGCAAGTCTATTACAGCAGCGCTTTTAGCGGGTGTATTTTTTCTAATTGGAAAAAGGTAAGTAGCAAGTGTTAGTTTTACAATAGGCAAGCATATTGGGTATAGACTAATTGTATGTTATTGTTTTCTTTTTCAGAAACAGCACCATAACAGGACCCAGGACAAGGATTGCATGCATCTTGACCC
droRho1	scf7180000763507:346-366 +		A-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGATGTCCTGCACCATGTCC-

Species

Coordinate

Alignment

droWil2

scf2_1100000004519:68364-68614 -

dwi_1192

ATACTAGTAATTAAAATAATTGTTTTCTTGCAGGTCATCAAATCGATCCACAGCGTTTTTGAAGACGGCAAGTCTATTACAGCAGCGCTTTTAGCGGGTGTATTTTTTCTAATTGGAAAAAGGTAAGTAGCAAGTGTTAGTTTTACAATAGGCAAGCATATTGGGTATAGACTAATTGTATGTTATTGTTTTCTTTTTCAGAAACAGCACCATAACAGGACCCAGGACAAGGATTGCATGCATCTTGACCC

droRho1

scf7180000763507:346-366 +

A-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGATGTCCTGCACCATGTCC-

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droWil2

ATACTAGTAATTAAAATAATTGTTTTCTTGCAGGTCATCAAATCGATCCACAGCGTTTTTGAAGACGGCAAGTCTATTACAGCAGCGCTTTTAGCGGGTGTATTTTTTCTAATTGGAAAAAGGTAAGTAGCAAGTGTTAGTTTTACAATAGGCAAGCATATTGGGTATAGACTAATTGTATGTTATTGTTTTCTTTTTCAGAAACAGCACCATAACAGGACCCAGGACAAGGATTGCATGCATCTTGACCC	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
..............................................................................................................................................................TATTGGGTATAGACTAATTGTATGT....................................................................	25	1	6.00	6	0	0	0	6	0
....................................................................................................................................................................................................................TAACAGGACCCAGGACAAGGATTGCA.............	26	2	4.50	9	3	3	0	3	0
....................................................................................................................................................................................................TTCAGAAACAGCACCATAACAGGACC.............................	26	1	3.00	3	0	2	0	1	0
..................................TCATCAAATCGATCCACAGCGTTTT................................................................................................................................................................................................	25	2	3.00	6	0	1	0	5	0
............................................................................................................CTAATTGGAAAAAGGTAAGTAGCAAG.....................................................................................................................	26	1	2.00	2	0	2	0	0	0
...............................................................................................................................TAGCAAGTGTTAGTTTTACAATAGGCA.................................................................................................	27	1	2.00	2	0	0	0	2	0
..............................................................................................................................................................................................................................CAGGACAAGGATTGCATGCATCTTGA...	26	2	1.50	3	0	2	0	1	0
....................................................................................................................................................................................................................TAACAGGACCCAGGACAAGGATTGC..............	25	2	1.50	3	0	0	0	3	0
.................................................................................................................................................................TGGGTATAGACTAATTGTATGTTAT.................................................................	25	2	1.50	3	0	0	0	2	1
...........................................................................................TAGCGGGTGTATTTTTTCTAATTGGAA.....................................................................................................................................	27	1	1.00	1	0	0	0	1	0
..............................................................................................................................................................TATTGGGTATAGACTAATTGTATGTT...................................................................	26	1	1.00	1	0	0	0	1	0
...........................................CGATCCACAGCGTTTTTGAAGACGG.......................................................................................................................................................................................	25	2	1.00	2	0	0	0	2	0
.................................................................................................................................................................TGGGTATAGACTAATTGTATGTTATTG...............................................................	27	2	1.00	2	0	0	0	2	0
....................................................................................................................................................................................................TTCAGAAACAGCACCATAACAGGACCCAG..........................	29	1	1.00	1	0	1	0	0	0
..................................................................................................................................CAAGTGTTAGTTTTACAATAGGCAAGCA.............................................................................................	28	1	1.00	1	0	1	0	0	0
...........................................................................................................................TAAGTAGCAAGTGTTAGTTTTACAATG.....................................................................................................	27	1	1.00	1	0	0	0	1	0
...........................................................................................................................TAAGTAGCAAGTGTTAGTTTTACAATA.....................................................................................................	27	1	1.00	1	0	0	0	1	0
.............................................................................................................................................................................................................AGCACCATAACAGGACCCAGGACAAG....................	26	2	1.00	2	0	1	0	1	0
....................TGTTTTCTTGCAGGTCATCAAATCGA.............................................................................................................................................................................................................	26	2	1.00	2	0	1	0	1	0
...............................................................................................................................................................................................TCTTTTTCAGAAACAGCACCATAACAG.................................	27	1	1.00	1	0	0	0	1	0
.........................................................................................................................................TAGTTTTACAATAGGCAAGCATAT..........................................................................................	24	1	1.00	1	0	1	0	0	0
............................TGCAGGTCATCAAATCGATCCACAGCG....................................................................................................................................................................................................	27	2	1.00	2	0	2	0	0	0
..........................................................................................................................................................AGCATATTGGGTATAGACTAATTG.........................................................................	24	1	1.00	1	0	0	0	1	0
...........................................................TGAAGACGGCAAGTCTATTACAGCAGC.....................................................................................................................................................................	27	1	1.00	1	0	1	0	0	0
...............................................................................................................................TAGCAAGTGTTAGTTTTACAATAGGC..................................................................................................	26	1	1.00	1	0	1	0	0	0
.........................................................................CTATTACAGCAGCGCTTTTAGCGGG.........................................................................................................................................................	25	1	1.00	1	0	1	0	0	0
......................................................................AGTCTATTACAGCAGCGCTTTTAG.............................................................................................................................................................	24	1	1.00	1	0	0	0	1	0
.................................................................................................................................................................................................................................GACAAGGATTGCATGCATCTTGACCC	26	2	1.00	2	0	2	0	0	0
......................................................................AGTCTATTACAGCAGCGCTTTTAGCGGGA........................................................................................................................................................	29	1	1.00	1	0	0	0	1	0
.........................TCTTGCAGGTCATCAAATCGATCCACA.......................................................................................................................................................................................................	27	2	1.00	2	0	2	0	0	0
..........................................................................TATTACAGCAGCGCTTTTAGCGGGT........................................................................................................................................................	25	1	1.00	1	0	0	0	1	0
........................................................................................TTTTAGCGGGTGTATTTTTTCTAATTG........................................................................................................................................	27	1	1.00	1	0	0	0	1	0
........................................................TTTTGAAGACGGCAAGTCTATTACAGC........................................................................................................................................................................	27	1	1.00	1	0	1	0	0	0
..............................................................................................................................................TTACAATAGGCAAGCATATTGGGTA....................................................................................	25	1	1.00	1	0	1	0	0	0
....................................................................................................................................................................................................TTCAGAAACAGCACCATAACAGGACCCA...........................	28	1	1.00	1	0	1	0	0	0
............................................................................................................................................................................TAATTGTATGTTATTGTTTTCTTT.......................................................	24	2	1.00	2	0	2	0	0	0
..........................................................................TATTACAGCAGCGCTTTTAGCGGGTG.......................................................................................................................................................	26	1	1.00	1	0	1	0	0	0
...........................................................................................................................................................................................................ACAGCACCATAACAGGACCCAGGAC.......................	25	2	0.50	1	0	0	0	1	0
............................................................................................................................................................................TAATTGTATGTTATTGTTTTCTT........................................................	23	2	0.50	1	0	0	0	1	0
...................................CATCAAATCGATCCACAGCGTTTT................................................................................................................................................................................................	24	2	0.50	1	0	0	0	1	0
.........................................ATCGATCCACAGCGTTTTTGAAGAC.........................................................................................................................................................................................	25	2	0.50	1	0	0	0	1	0
............................TGCAGGTCATCAAATCGATCCACAGCGT...................................................................................................................................................................................................	28	2	0.50	1	0	0	0	1	0
....................................................................................................................................................................................................................TAACAGGACCCAGGACAAGGAT.................	22	2	0.50	1	0	0	0	1	0
........................................................TTTTGAAGACGGCAAGTCTATTACA..........................................................................................................................................................................	25	2	0.50	1	0	1	0	0	0
....................................................................................................................................................................................................................TAACAGGACCCAGGACAAGGATTGT..............	25	2	0.50	1	0	1	0	0	0
..................................................................................................................................................................................................................CATAACAGGACCCAGGACAAGGATTGCA.............	28	2	0.50	1	0	1	0	0	0
.....................................................................................................................................................................................................................AACAGGACCCAGGACAAGGATTGCA.............	25	2	0.50	1	0	0	0	1	0
..................................................................................................................................................................................................................CATAACAGGACCCAGGACAAGGATTG...............	26	2	0.50	1	0	0	0	1	0
..............................................TCCACAGCGTTTTTGAAGACGGCAAG...................................................................................................................................................................................	26	2	0.50	1	0	1	0	0	0
............................................................................................................................................................................................................................CCCAGGACAAGGATTGCATGCATCT......	25	2	0.50	1	0	0	0	1	0
....................................................................................................................................................................GTATAGACTAATTGTATGTTATTGTT.............................................................	26	2	0.50	1	0	1	0	0	0
.............................................................................................................................................................................................................................CCAGGACAAGGATTGCATGCATCTTGA...	27	2	0.50	1	0	0	0	1	0
....................................................................................................................................................................................................................TAACAGGACCCAGGACAAGGATTG...............	24	2	0.50	1	0	1	0	0	0
.....................................................................................................................................................................TATAGACTAATTGTATGTTATTGT..............................................................	24	2	0.50	1	0	0	0	1	0
.........................TCTTGCAGGTCATCAAATCGATCCAC........................................................................................................................................................................................................	26	2	0.50	1	0	1	0	0	0
.........ATTAAAATAATTGTTTTCTTGCAGG.........................................................................................................................................................................................................................	25	2	0.50	1	0	1	0	0	0
.................................................................................................................................................................TGGGTATAGACTAATTGTATGT....................................................................	22	2	0.50	1	0	0	0	1	0
................................................................................................................................................................TTGGGTATAGACTAATTGTATGTTA..................................................................	25	2	0.50	1	0	1	0	0	0
.....................................................................................................................................................................TATAGACTAATTGTATGTTATTGTTT............................................................	26	2	0.50	1	0	1	0	0	0
........................TTCTTGCAGGTCATCAAATCGATCCACA.......................................................................................................................................................................................................	28	2	0.50	1	0	1	0	0	0
................................................................................................................................................................TTGGGTATAGACTAATTGTATGTTAT.................................................................	26	2	0.50	1	0	0	0	1	0
....................TGTTTTCTTGCAGGTCATCAAATCGAT............................................................................................................................................................................................................	27	2	0.50	1	0	0	0	1	0
..................................................................................................................................................................................................................CATAACAGGACCCAGGACAAGGATTGC..............	27	2	0.50	1	0	0	0	1	0
............................................................................................................................................................................................................CAGCACCATAACAGGACCCAGGACAA.....................	26	2	0.50	1	0	0	0	1	0
........................................AATCGATCCACAGCGTTTTTGAAGACGG.......................................................................................................................................................................................	28	2	0.50	1	0	1	0	0	0
..........................................TCGATCCACAGCGTTTTTGAAGACGG.......................................................................................................................................................................................	26	2	0.50	1	0	0	0	1	0
.......................................AAATCGATCCACAGCGTTTTTGAAGA..........................................................................................................................................................................................	26	2	0.50	1	0	0	0	1	0
..................................................................................................................................................................................................................CATAACAGGACCCAGGACAAGGAT.................	24	2	0.50	1	0	1	0	0	0
.............................................................................................................................................................................................................AGCACCATAACAGGACCCAGGACAA.....................	25	2	0.50	1	0	1	0	0	0
............................................................................................................................................................................TAATTGTATGTTATTGTTTTCTTTTT.....................................................	26	2	0.50	1	0	1	0	0	0
..........................................................................................................................................................................................................................GACCCAGGACAAGGATTGCATGCATC.......	26	2	0.50	1	0	0	0	0	1
..............................CAGGTCATCAAATCGATCCACAGCGTT..................................................................................................................................................................................................	27	2	0.50	1	0	1	0	0	0
..............................................................................................................................................................................................................................CAGGACAAGGATTGCATGCATCTT.....	24	2	0.50	1	0	0	0	1	0
.....................................TCAAATCGATCCACAGCGTTTTTGA.............................................................................................................................................................................................	25	2	0.50	1	0	0	0	1	0
.................................................................................................................................................................TGGGTATAGACTAATTGTATGTTATT................................................................	26	2	0.50	1	0	0	0	1	0
..............................................TCCACAGCGTTTTTGAAGACGGCAAGTC.................................................................................................................................................................................	28	2	0.50	1	0	1	0	0	0
.....................................TCAAATCGATCCACAGCGTTTTTGAAG...........................................................................................................................................................................................	27	2	0.50	1	0	0	0	1	0
.......................TTTCTTGCAGGTCATCAAATCGATC...........................................................................................................................................................................................................	25	2	0.50	1	0	1	0	0	0
.............................................................................................................................................................................................................AGCACCATAACAGGACCCAGGACAAGGA..................	28	2	0.50	1	0	1	0	0	0

droRho1

A-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGATGTCCTGCACCATGTCC-	Size	Hit Count	Total Norm	Total
No Reads

dwi_1192

scf2_1100000004519:68414-68564 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-8.8	-8.3	-8.2