ID:

dwi_100

Coordinate:

scf2_1100000004909:4979080-4979169 +

Confidence:

candidate

Class:

Canonical miRNA

Genomic Locale:

intron

[View on UCSC Genome Browser {Cornell Mirror}]

Legend:

mature

star

mismatch in alignment

mismatch in read

Predicted structure

-30.6	-30.4	-30.3

Flybase annnotation

intron [Dwil\GK25647-in]

No Repeatable elements found

Sense Strand Reads

TTTCTATACTTTTTTAGTTAGGAATAATAAATGATAAACTTGATGCCCCTTGAAGTATGCAATGAACATAAAGAACGTTTTAAATTGTCAAATAACTTAAAATTAATAACAATTTATGCCCTTTGCATACTTTAGGGGACATTTCATATCCCTTGACATCGTTTTTTATATGTATTGATGTTATGTGTTT *********************************......((((.((((((((((((((((....((((((....(((((((.((((...)))).))))))).........))))))....)))))))))))))))).))))...........**********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V119 head	M020 head	V118 embryo	V117 male body
..................................................TGAAGTATGCAATGAACATA........................................................................................................................	20	0	1	24.00	24	16	7	1	0
..................................................TGAAGTATGCAATGAACAT.........................................................................................................................	19	0	1	11.00	11	3	8	0	0
..................................................TGAAGTATGCAATGAACATAA.......................................................................................................................	21	0	1	10.00	10	0	0	9	1
..................................................TGAAGTATGCAATGAACATAAA......................................................................................................................	22	0	1	2.00	2	0	0	2	0
..................................................CGAAGTATGCAATGAACATA........................................................................................................................	20	1	1	1.00	1	1	0	0	0
...................................................................................................................ATGCCCTTTGCATACTTTAGGGGA...................................................	24	0	1	1.00	1	0	0	1	0
..................................................TGAAGTATGCAATGAATATA........................................................................................................................	20	1	1	1.00	1	1	0	0	0
..................................................TAAAGTATGCAATGAACATAA.......................................................................................................................	21	1	2	0.50	1	0	0	1	0
..................................................TGGAGTATGCAATGAACAT.........................................................................................................................	19	1	15	0.07	1	1	0	0	0

Anti-sense strand reads

AAAGATATGAAAAAATCAATCCTTATTATTTACTATTTGAACTACGGGGAACTTCATACGTTACTTGTATTTCTTGCAAAATTTAACAGTTTATTGAATTTTAATTATTGTTAAATACGGGAAACGTATGAAATCCCCTGTAAAGTATAGGGAACTGTAGCAAAAAATATACATAACTACAATACACAAA **********************************......((((.((((((((((((((((....((((((....(((((((.((((...)))).))))))).........))))))....)))))))))))))))).))))...........*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Type	Alignment
droWil2	scf2_1100000004909:4979030-4979219 +	dwi_100	candidate	TTTCTATACTTTTTTAGTTAGGAATAATAAATGATAAACTTGATGCCCCTTGAAGTATGCAATGAACATAAAGAACGTTTTAAATTGTCAAATAACTTAAAATTAATAACAATTTATGCCCTTTGCATACTTTAGGGGACATTTCATATCCCTTGACATCGTTTTTTATATGTATTGATGTTATGTGTTT------------
droMoj3	scaffold_6328:147402-147471 +			TT-----------------------------------------------------------------------------------------------------------------------------------AGGCAACTGCTCAGGTTTTCTTACTTTGTAGTCTTTATAAGTTGAGTTGTTCG-CCTGCACAGAGAAAC
droAna3	scaffold_13117:3765920-3765921 +			TT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droKik1	scf7180000302586:169398-169424 +			TTTTCTTATTTATTT----------------------------------------------------------------------------------------------------------------------------------------------------------ATGAT---------TTTATTT------------
droRho1	scf7180000779914:607735-607759 -			TTTT---------------------------------------------------------------------------------------------------------------------------------------------------------------------ATCTTATAGCATTCTGTCTTT------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droWil2

TTTCTATACTTTTTTAGTTAGGAATAATAAATGATAAACTTGATGCCCCTTGAAGTATGCAATGAACATAAAGAACGTTTTAAATTGTCAAATAACTTAAAATTAATAACAATTTATGCCCTTTGCATACTTTAGGGGACATTTCATATCCCTTGACATCGTTTTTTATATGTATTGATGTTATGTGTTT------------	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
..................................................TGAAGTATGCAATGAACATA....................................................................................................................................	20	1	24.00	24	7	0	0	1	16
..................................................TGAAGTATGCAATGAACAT.....................................................................................................................................	19	1	11.00	11	8	0	0	0	3
..................................................TGAAGTATGCAATGAACATAA...................................................................................................................................	21	1	10.00	10	0	0	1	9	0
..................................................TGAAGTATGCAATGAACATAAA..................................................................................................................................	22	1	2.00	2	0	0	0	2	0
...................................................................................................................ATGCCCTTTGCATACTTTAGGGGA...............................................................	24	1	1.00	1	0	0	0	1	0

droMoj3

TT-----------------------------------------------------------------------------------------------------------------------------------AGGCAACTGCTCAGGTTTTCTTACTTTGTAGTCTTTATAAGTTGAGTTGTTCG-CCTGCACAGAGAAAC	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
No Reads

droAna3

TT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
No Reads

droKik1

TTTTCTTATTTATTT----------------------------------------------------------------------------------------------------------------------------------------------------------ATGAT---------TTTATTT------------	Size	Hit Count	Total Norm	Total
No Reads

droRho1

TTTT---------------------------------------------------------------------------------------------------------------------------------------------------------------------ATCTTATAGCATTCTGTCTTT------------	Size	Hit Count	Total Norm	Total
No Reads

Generated: 10/20/2015 at 06:58 PM