dvi_9866

##################################################--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- TGTAATTGTCGCCGGCGGTATGCCCAATGTGCCAACATCGAATTTAAATAGTAAGTAGAACCGCCCACAAAACGCGCTATTTCAGATTACAAATTGCAAATGTTTATTTACATTTGTTTTTCAGATTTTTAGTAAGCTTTTTGTGGAGAAAACATATTCAATTATATAATAAAAAGCTCAATTATATTATTATTGCTTCAATACGAAATTGTTTAGATAGATTTAATGTATTTATTTAGAAAAAATTGAAA ************************************************..((((((......((((((((.((..((((..(((((..(((..((((((((......)))))))).)))..)))))..)))).)).)))))))).(((......)))(((.(((((((..........))))))).))).))))))...************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V116 male body	SRR060684 140x9_0-2h_embryos_total
........................................................................................................................................................................................TATTATTATTGCTTCAGT.................................................	18	1	5	0.20	1	1	0
...................................................................................................................................................................................................................................GTACTTATTTAGCAAAAAT.....	19	2	15	0.07	1	0	1

Species	Coordinate	ID	Alignment
droVir3	scaffold_12963:393931-394181 -	dvi_9866	TGTA---ATTGTCGCCGGC---------------GGTAT------GCCCAATGTGCCA--ACATCGAATTTAAATAGTAAGTA-------------------------GAACCGCCCACA--AAACGCGCTATTTCAGATTACAAATTGCAAATGTTTATTTACATTTGTTTTTCAGATTTTTAGTAAGCTTTTTGTGGAG---------------------------------------------------------------------------AAAACATATTCAATTATATAATAAAAAGCTCAA------------TTATATTATTATTGCTT--------------------------------------CAATACGAAATTGTTTAGATAGATTTAATGTATTTAT-----------------------------------------------TTAGA------------AAAAATTGAA---A-----------------------------------------
droMoj3	scaffold_6496:19032388-19032493 +		AATG---TTTAATTTAATT---------------TTTTT------TTCTAAT-------------------------------------------------------------------------------------------------------------------------------TTTTATTTAATTTTTTGTTTAGTAT----------------------------------------------------------------------------TTTACATAATTATACTCTAAAAAAAACA------------------------------------------------------------------------------------------------------------GAAATTTTC-----------------------------------CTAGA------------ATTTTTTTAA---T-----------------------------------------
droGri2	scaffold_8549:4341-4623 -		CATA---ATTGTCGCCGGC---------------GGTGT------GCCCAATGTGCCA--ACATCGAATTTAAATGGTAAGCA-------------------------CGCC---CCACAATCAACTCTCAATAGCTACTTCAAGACATCAAAT-------------------------AAATATTGCATTTTTAGAAT-----------------------------------------------------------------------------ATACAATAGTTGATAATATCAAAAAGAGAACAGAGTTTATTATTTTTAAATTATAACTACTTTAAAT-----ATATGCATATCTGTTTAATTT--------------------------------------------ATCGGAAGTAATATTTGTTTAGATCAGATGTGTCAGCATCAGATTATTTAGCATAGTTAGAGGTGTAAATTAAA---A-----------------------------------------
droWil2	scf2_1100000003103:9455-9628 -		TTTC---TTTACGATTTTT---------------TTTAA------TTACAATTTTTTT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAATTGCCTTAATTTTACCTATAATTATTATTTTAAAATTTTCTAATTT---------------------------------ATTGATTTG--------------------------------AAG---AATTACTTCAATT-----ATTTTTATTTTAATTTTATTA--------------------------------------------ACTGGATTATAC-----------------------------------CATGA------------ATGAAATCAA---G-----------------------------------------
dp5	4_group5:1360683-1360745 -		CGTG---ATTGTTGCCGGT---------------GGCGGTGTTGTGGCCAATGGCCCA--ACCACGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droPer2	scaffold_5:5766531-5766593 -		CGTG---ATTGTTGCCGGT---------------GGCGGAGTTGTGGCCAATGGCCCA--ACCACGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droAna3	scaffold_12943:404819-404875 -		CGTG---ATTGTTTCCGGG---------------GGCGT------GGCCAATGCGCCG--ACCTCGAATTTAAACAGTAAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droBip1	scf7180000396535:311775-311927 +		CGTG---ATTGTTTCCGGG---------------GGCGT------GGCCAATGGGCCG--ACCTCGAATTTAAACAGTAAGTA-------------------------TTTC---GGAT------------------------------------------------------------GCATATCTAATA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CACTCACTTAAAATTACAC-----------------------------------TCTGG------------ATAAAAGCCA---AATTTATAAATGAATTTGTTTAATAAATATTTTGAGCTATTT
droKik1	scf7180000302684:642857-642913 -		CCTG---ATTGTTGCCGGC---------------GGCGT------GGCCAATGTGCCG--ACCTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droFic1	scf7180000448985:144-200 +		CGTG---ATTGTGGCCGGA---------------GGCGG------GGCCAATGTGCCG--ACCTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEle1	scf7180000491186:1969020-1969076 -		CGTG---ATTGTGGCCGGA---------------GGCGG------GGCCAATGTGCCG--ACCTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droRho1	scf7180000777214:191085-191141 +		CGTG---ATTGTGGCCGGA---------------GGCGG------GGCCAATGTGCCG--ACCTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droBia1	scf7180000302408:4168071-4168127 +		CGTG---ATTGTGGCCGGA---------------GGCGG------GGCCAATGTGCCG--ACTTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droTak1	scf7180000415702:488596-488652 +		CGTG---ATTGTGGCCGGA---------------GGCGG------GGCCAATGTGCCG--ACTTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEug1	scf7180000408162:6074-6380 +		TGTCTACATTATCGCCAAATAAAACAAATAAATCCGCGA------CACCTAACTGTGGGAACATTA-------------AGAATTCCCTCTCTTTGCAATTTTGAGTAAAATCG----------ACTT----TTCT------------------------------------------------CGAAAATAAAAACTTATGAATTAAATAAATTTT--------TTTTTATTTTAAT-TTTTCTTATTTCTCTCGAATATAAAAAACCTTTGAAAAAAAAAAATTTACTTA--------------------------------AACAATACTTAAATTAAGTAAAAAGCTCTTATTTGAATTTTTCTGGGTGTTCCAAAGAGAACATATTAAAACAC---AAATATATTTAT-----------------------------------------------CTAGC------------AGATTCTAAAATAA-----------------------------------------
dm3	chr2L:9066073-9066129 -		CGTG---ATTGTGGCCGGA---------------GGCGG------GGCCAATGTGCCG--ACCTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droSim2	2l:8764612-8764668 -		CGTG---ATTGTGGCCGGA---------------GGCGG------GGCCAATGTGCCG--ACCTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droSec2	scaffold_3:4532120-4532176 -		CGTG---ATTGTGGCCGGA---------------GGCGG------GGCCAATGTGCCG--ACCTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droYak3	2L:11726324-11726380 -		CGTG---ATTGTGGCCGGA---------------GGCGG------GGCCAATGTGCCG--ACCTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEre2	scaffold_4929:9666977-9667033 -		CGTG---ATTGTGGCCGGC---------------GGCGG------GGCCAATGTGCCG--ACCTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droVir3

TGTA---ATTGTCGCCGGC---------------GGTAT------GCCCAATGTGCCA--ACATCGAATTTAAATAGTAAGTA-------------------------GAACCGCCCACA--AAACGCGCTATTTCAGATTACAAATTGCAAATGTTTATTTACATTTGTTTTTCAGATTTTTAGTAAGCTTTTTGTGGAG---------------------------------------------------------------------------AAAACATATTCAATTATATAATAAAAAGCTCAA------------TTATATTATTATTGCTT--------------------------------------CAATACGAAATTGTTTAGATAGATTTAATGTATTTAT-----------------------------------------------TTAGA------------AAAAATTGAA---A-----------------------------------------

Size

Hit Count

Total Norm

Total

M027
Male-body

M028
Head

M047
Female-body

M061
Embryo

SRR060654
Ovary

SRR060655
Testis

SRR060656
Ovary

SRR060657
Testis

SRR060658
Ovary

SRR060659
Testis

SRR060660
Ovary

SRR060661
Embryo

SRR060662
Embryo

SRR060663
Embryo

SRR060664
Male-body

SRR060665
Female-body

SRR060666
Male-body

SRR060667
Female-body

SRR060668
Male-body

SRR060669
Female-body

SRR060670
Testis

SRR060671
Male-body

SRR060672
Female-body

SRR060673
Ovary

SRR060674
Ovary

SRR060675
Ovary

SRR060676
Ovary

SRR060677
Ovary

SRR060678
Testis

SRR060679
Testis

SRR060680
Testis

SRR060681
Testis

SRR060682
Embryo

SRR060683
Testis

SRR060684
Embryo

SRR060685
Embryo

SRR060686
Embryo

SRR060687
Embryo

SRR060688
Ovary

SRR060689
Testis

SRR1106712
embryo_0-2h

SRR1106713
embryo_0-2h

SRR1106714
embryo_2-4h

SRR1106715
embryo_4-6h

SRR1106716
embryo_4-6h

SRR1106717
embryo_6-8h

SRR1106718
embryo_6-8h

SRR1106719
embryo_8-10h

SRR1106720
embryo_8-10h

SRR1106721
embryo_10-12h

SRR1106722
embryo_10-12h

SRR1106723
embryo_12-14h

SRR1106724
embryo_12-14h

SRR1106725
embryo_14-16h

SRR1106726
embryo_14-16h

SRR1106727
larvae

SRR1106728
larvae

SRR1106729
mixed whole adult body

SRR1106730
embryo_16-30h

V047
Embryo

V053
Head

V116
Male-body

GSM1528803
follicle cells

No Reads

droMoj3

AATG---TTTAATTTAATT---------------TTTTT------TTCTAAT-------------------------------------------------------------------------------------------------------------------------------TTTTATTTAATTTTTTGTTTAGTAT----------------------------------------------------------------------------TTTACATAATTATACTCTAAAAAAAACA------------------------------------------------------------------------------------------------------------GAAATTTTC-----------------------------------CTAGA------------ATTTTTTTAA---T-----------------------------------------	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
No Reads

droGri2

CATA---ATTGTCGCCGGC---------------GGTGT------GCCCAATGTGCCA--ACATCGAATTTAAATGGTAAGCA-------------------------CGCC---CCACAATCAACTCTCAATAGCTACTTCAAGACATCAAAT-------------------------AAATATTGCATTTTTAGAAT-----------------------------------------------------------------------------ATACAATAGTTGATAATATCAAAAAGAGAACAGAGTTTATTATTTTTAAATTATAACTACTTTAAAT-----ATATGCATATCTGTTTAATTT--------------------------------------------ATCGGAAGTAATATTTGTTTAGATCAGATGTGTCAGCATCAGATTATTTAGCATAGTTAGAGGTGTAAATTAAA---A-----------------------------------------	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

droWil2

TTTC---TTTACGATTTTT---------------TTTAA------TTACAATTTTTTT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAATTGCCTTAATTTTACCTATAATTATTATTTTAAAATTTTCTAATTT---------------------------------ATTGATTTG--------------------------------AAG---AATTACTTCAATT-----ATTTTTATTTTAATTTTATTA--------------------------------------------ACTGGATTATAC-----------------------------------CATGA------------ATGAAATCAA---G-----------------------------------------	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
..............................................................................................................................................................................................TTTTGATGTAGAAATTGCC...................................................................................................................................................................................................................................................................................................................................	19	3	48.67	146	129	2	0	14	1
.............................................................................................................................................................................................TTTTTGATGTAGAAATTGCC...................................................................................................................................................................................................................................................................................................................................	20	3	31.00	93	85	2	0	5	1
...............................................................................................................................................................................................TTTGATGTAGAAATTGCC...................................................................................................................................................................................................................................................................................................................................	18	3	30.67	92	89	0	0	3	0
............................................................................................................................................................................................ATTTTTGATGTAGAAATTGCC...................................................................................................................................................................................................................................................................................................................................	21	3	26.33	79	72	2	0	4	1
..............................................................................................................................................................................................TTTTGATGTAGAAATTGCCT..................................................................................................................................................................................................................................................................................................................................	20	3	19.67	59	52	1	0	6	0
............................................................................................................................................................................................ATTTTTGATGTAGAAATTGCCT..................................................................................................................................................................................................................................................................................................................................	22	3	13.33	40	35	1	2	2	0
.............................................................................................................................................................................................TTTTTGATGTAGAAATTGCCT..................................................................................................................................................................................................................................................................................................................................	21	3	12.67	38	34	0	0	4	0
...............................................................................................................................................................................................TTTGATGTAGAAATTGCCT..................................................................................................................................................................................................................................................................................................................................	19	3	10.00	30	29	0	0	1	0
..............................................................................................................................................................................................TTTTGATGTAGAAATTGCCTT.................................................................................................................................................................................................................................................................................................................................	21	3	9.33	28	11	1	0	16	0
................................................................................................................................................................................................TTGATGTAGAAATTGCCT..................................................................................................................................................................................................................................................................................................................................	18	3	8.33	25	24	0	0	1	0
...............................................................................................................................................................................................TTTGATGTAGAAATTGCCTT.................................................................................................................................................................................................................................................................................................................................	20	3	6.33	19	6	1	0	12	0
...........................................................................................................................................................................................AATTTTTGATGTAGAAATTGCC...................................................................................................................................................................................................................................................................................................................................	22	3	5.00	15	8	0	0	6	1
...........................................................................................................................................................................................AATTTTTGATGTAGAAATTGCCT..................................................................................................................................................................................................................................................................................................................................	23	3	5.00	15	5	2	0	7	1
...........................................................................................................................................................................................AATTTTTGATGTAGAAAT.......................................................................................................................................................................................................................................................................................................................................	18	4	4.00	16	4	0	0	12	0
............................................................................................................................................................................................ATTTTTGATGTAGAAATTGCCTT.................................................................................................................................................................................................................................................................................................................................	23	3	3.00	9	5	0	0	4	0
..................................................................................................................................................................................................GATGTAGAAATTGCCTTAAT..............................................................................................................................................................................................................................................................................................................................	20	3	2.67	8	0	0	0	7	1
..............................................................................................................................................................................................TTTTGATGTAGAAATTGCCTTAAT..............................................................................................................................................................................................................................................................................................................................	24	3	2.67	8	3	0	0	4	1
.............................................................................................................................................................................................TTTTTGATGTAGAAATTGC....................................................................................................................................................................................................................................................................................................................................	19	4	2.50	10	2	0	0	6	2
...........................................................................................................................................................................................AATTTTTGATGTAGAAATT......................................................................................................................................................................................................................................................................................................................................	19	4	2.50	10	6	0	0	4	0
...........................................................................................................................................................................................AATTTTTGATGTAGAAATTGCCA..................................................................................................................................................................................................................................................................................................................................	23	3	2.33	7	4	0	0	3	0
..................................................................................................................................................................................................GATGTAGAAATTGCCTTAATT.............................................................................................................................................................................................................................................................................................................................	21	3	2.33	7	0	1	0	6	0
.............................................................................................................................................................................................TTTTTGATGTAGAAATTGCCTT.................................................................................................................................................................................................................................................................................................................................	22	3	2.33	7	4	0	1	2	0
...........................................................................................................................................................................................AATTTTTGATGTAGAAATTGC....................................................................................................................................................................................................................................................................................................................................	21	4	2.00	8	3	0	0	5	0
.........................................................T------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGA..........................................................................................................................................................................................................................................................................................................................................	19	4	2.00	8	0	2	0	6	0
..............................................................................................................................................................................................TTTTGATGTAGAAATTGCCTTA................................................................................................................................................................................................................................................................................................................................	22	3	2.00	6	1	0	0	5	0
...........................................................................................................................................................................................AATTTTTGATGTAGAAATTGCCTTAAT..............................................................................................................................................................................................................................................................................................................................	27	3	2.00	6	0	2	1	3	0
.....................................................................................................................................................................................................................................................................................................................................................................................................................................ATAC-----------------------------------CATGA------------ATGAAATCA..............................................	18	4	1.75	7	0	0	0	7	0
........................................................................................................................................................................................TTTAATTTTTGATGTAGA..........................................................................................................................................................................................................................................................................................................................................	18	4	1.75	7	0	0	0	7	0
................................................................................................................................................................................................TTGATGTAGAAATTGCCTT.................................................................................................................................................................................................................................................................................................................................	19	3	1.67	5	2	0	0	3	0
................................................................................................................................................................................................TTGATGTAGAAATTGCCA..................................................................................................................................................................................................................................................................................................................................	18	3	1.67	5	5	0	0	0	0
............................................................................................................................................................................................ATTTTTGATGTAGAAATTGCCTTA................................................................................................................................................................................................................................................................................................................................	24	3	1.67	5	3	1	0	1	0
........................................................................................................................................................................................TTTAATTTTTGATGTAGAAATTGCC...................................................................................................................................................................................................................................................................................................................................	25	3	1.67	5	4	0	0	1	0
..............................................................................................................................................................................................TTTTGATGTAGAAATTGCCTTAATT.............................................................................................................................................................................................................................................................................................................................	25	3	1.67	5	0	0	0	3	2
....................................................................................................................................................................................................................................................................................................................................................................................................................................TATAC-----------------------------------CATGA------------ATGAAATCA..............................................	19	4	1.50	6	0	1	0	5	0
.......................................................TTT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGA..........................................................................................................................................................................................................................................................................................................................................	21	4	1.50	6	0	0	0	5	1
........................................................TT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGA..........................................................................................................................................................................................................................................................................................................................................	20	4	1.50	6	1	0	0	5	0
.........................................................T------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAAT.......................................................................................................................................................................................................................................................................................................................................	22	4	1.50	6	1	0	1	4	0
..................................................................................................................................................................................................GATGTAGAAATTGCCTTA................................................................................................................................................................................................................................................................................................................................	18	3	1.33	4	0	2	0	2	0
..........................................................................................................................................................................................TAATTTTTGATGTAGAAATTGCC...................................................................................................................................................................................................................................................................................................................................	23	3	1.33	4	2	2	0	0	0
.................................................................................................................................................................................................TGATGTAGAAATTGCCTT.................................................................................................................................................................................................................................................................................................................................	18	3	1.33	4	3	0	0	1	0
..............................................................................................................................................................................................TTTTGATGTAGAAATTGCCA..................................................................................................................................................................................................................................................................................................................................	20	3	1.33	4	4	0	0	0	0
.......................................................TTT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAATTGCC...................................................................................................................................................................................................................................................................................................................................	28	3	1.33	4	2	2	0	0	0
..............................................................................................................................................................................................TTTTGATGTAGAAATTGCCC..................................................................................................................................................................................................................................................................................................................................	20	3	1.33	4	4	0	0	0	0
.............................................................................................................................................................................................TTTTTGATGTAGAAATTGCCC..................................................................................................................................................................................................................................................................................................................................	21	3	1.33	4	4	0	0	0	0
..............................................................................................................................................................................................TTTTGATGTAGAAATTGCCTTAA...............................................................................................................................................................................................................................................................................................................................	23	3	1.33	4	0	0	0	4	0
.........................................................................................................................................................................................TTAATTTTTGATGTAGAAATTGCCT..................................................................................................................................................................................................................................................................................................................................	25	3	1.33	4	2	0	0	2	0
............................................................................................................................................................................................ATTTTTGATGTAGAAATTGCCA..................................................................................................................................................................................................................................................................................................................................	22	3	1.00	3	3	0	0	0	0
...............................................................................................................................................................................................TTTGATGTAGAAATTGCCTTAAT..............................................................................................................................................................................................................................................................................................................................	23	3	1.00	3	1	0	0	2	0
.........................................................T------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAATTGCCTT.................................................................................................................................................................................................................................................................................................................................	28	3	1.00	3	3	0	0	0	0
........................................................................................................................................................................................TTTAATTTTTGATGTAGAAATTGCCT..................................................................................................................................................................................................................................................................................................................................	26	3	1.00	3	2	0	0	1	0
.............................................................................................................................................................................................TTTTTGATGTAGAAATTGCCA..................................................................................................................................................................................................................................................................................................................................	21	3	1.00	3	3	0	0	0	0
..............................................................................................................................................................................................TTTTGATGTAGAAATTGC....................................................................................................................................................................................................................................................................................................................................	18	4	1.00	4	0	0	0	4	0
................................................................................................................................................................................................TTGATGTAGAAATTGCCC..................................................................................................................................................................................................................................................................................................................................	18	3	1.00	3	3	0	0	0	0
..........................................................................................................................................................................................TAATTTTTGATGTAGAAAT.......................................................................................................................................................................................................................................................................................................................................	19	4	1.00	4	1	0	0	3	0
.........................................................T------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAA.........................................................................................................................................................................................................................................................................................................................................	20	4	1.00	4	0	0	0	4	0
............................................................................................................................................................................................ATTTTTGATGTAGAAATT......................................................................................................................................................................................................................................................................................................................................	18	4	1.00	4	2	0	0	2	0
......................................................TTTT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGT.............................................................................................................................................................................................................................................................................................................................................	19	4	1.00	4	0	0	0	4	0
.........................................................................................................................................................................................TTAATTTTTGATGTAGAAATTGCC...................................................................................................................................................................................................................................................................................................................................	24	3	1.00	3	3	0	0	0	0
..................................................................................................................................................................................................GATGTAGAAATTGCCTTAATTTTACC........................................................................................................................................................................................................................................................................................................................	26	3	1.00	3	0	1	0	2	0
...........................................................................................................................................................................................AATTTTTGATGTAGAAATTGCCTT.................................................................................................................................................................................................................................................................................................................................	24	3	1.00	3	0	2	0	1	0
...............................................................................................................................................................................................TTTGATGTAGAAATTGCCTTA................................................................................................................................................................................................................................................................................................................................	21	3	1.00	3	3	0	0	0	0
.........................................................................................................................................................................................TTAATTTTTGATGTAGAAATT......................................................................................................................................................................................................................................................................................................................................	21	4	0.75	3	2	0	0	1	0
......................................................TTTT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAAT.......................................................................................................................................................................................................................................................................................................................................	25	4	0.75	3	0	0	0	3	0
..........................................................................................................................................................................................TAATTTTTGATGTAGAAATT......................................................................................................................................................................................................................................................................................................................................	20	4	0.75	3	2	0	0	1	0
........................................................................................................................................................................................TTTAATTTTTGATGTAGAAAT.......................................................................................................................................................................................................................................................................................................................................	21	4	0.75	3	0	0	0	3	0
.......................................................TTT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTA............................................................................................................................................................................................................................................................................................................................................	19	4	0.75	3	0	0	0	3	0
............................................................................................................................................................................................ATTTTTGATGTAGAAATTGC....................................................................................................................................................................................................................................................................................................................................	20	4	0.75	3	0	0	0	3	0
..........................................................................................................................................................................................TAATTTTTGATGTAGAAA........................................................................................................................................................................................................................................................................................................................................	18	4	0.75	3	0	0	0	3	0
.........................................................................................................................................................................................TTAATTTTTGATGTAGAAATTGC....................................................................................................................................................................................................................................................................................................................................	23	4	0.75	3	2	0	0	1	0
.......................................................TTT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAA.........................................................................................................................................................................................................................................................................................................................................	22	4	0.75	3	2	0	0	1	0
.......................................................TTT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAAT.......................................................................................................................................................................................................................................................................................................................................	24	4	0.75	3	0	0	0	3	0
........................................................TT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAAT.......................................................................................................................................................................................................................................................................................................................................	23	4	0.75	3	0	1	0	2	0
..........................................................................................................................................................................................TAATTTTTGATGTAGAAATTGCCT..................................................................................................................................................................................................................................................................................................................................	24	3	0.67	2	2	0	0	0	0
..................................................................................................................................................................................................GATGTAGAAATTGCCTTAATTTTAAA........................................................................................................................................................................................................................................................................................................................	26	3	0.67	2	0	0	0	2	0
...............................................................................................................................................................................................TTTGATGTAGAAATTGCCTTAA...............................................................................................................................................................................................................................................................................................................................	22	3	0.67	2	0	1	0	1	0
...................................................................................................................................................................................................ATGTAGAAATTGCCTTAAA..............................................................................................................................................................................................................................................................................................................................	19	3	0.67	2	0	0	0	2	0
.........................................................T------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAATTGCC...................................................................................................................................................................................................................................................................................................................................	26	3	0.67	2	1	0	0	1	0
.........................................................T------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAATTGCCA..................................................................................................................................................................................................................................................................................................................................	27	3	0.67	2	0	0	0	2	0
..................................................................................................................................................................................................GATGTAGAAATTGCCTTAATTT............................................................................................................................................................................................................................................................................................................................	22	3	0.67	2	0	0	1	1	0
.............................................................................................................................................................................................TTTTTGATGTAGAAATTGCCTTAAT..............................................................................................................................................................................................................................................................................................................................	25	3	0.67	2	0	0	1	1	0
...............................................................................................................................................................................................TTTGATGTAGAAATTGCCC..................................................................................................................................................................................................................................................................................................................................	19	3	0.67	2	2	0	0	0	0
...........................................................................................................................................................................................AATTTTTGATGTAGAAATTGCCTTA................................................................................................................................................................................................................................................................................................................................	25	3	0.67	2	0	2	0	0	0
............................................................................................................................................................................................ATTTTTGATGTAGAAATTGCCTTAAT..............................................................................................................................................................................................................................................................................................................................	26	3	0.67	2	1	0	0	1	0
................................................................................................................................................................................................TTGATGTAGAAATTGCCTTAAT..............................................................................................................................................................................................................................................................................................................................	22	3	0.67	2	1	0	0	1	0
....................................................................................................................................................................................................TGTAGAAATTGCCTTAAT..............................................................................................................................................................................................................................................................................................................................	18	3	0.67	2	0	0	0	2	0
...............................................................................................................................................................................................TTTGATGTAGAAATTGCCTTAATTT............................................................................................................................................................................................................................................................................................................................	25	3	0.67	2	0	0	0	2	0
.................................................................................................................................................................................................TGATGTAGAAATTGCCTTAAT..............................................................................................................................................................................................................................................................................................................................	21	3	0.67	2	0	0	0	0	2
.................................................................................................................................................................................................TGATGTAGAAATTGCCTTAATT.............................................................................................................................................................................................................................................................................................................................	22	3	0.67	2	1	0	0	1	0
..........................................................................................................................................................................................TAATTTTTGATGTAGAAATTGCCTT.................................................................................................................................................................................................................................................................................................................................	25	3	0.67	2	1	0	0	1	0
..................................................................................................................................................................................................GATGTAGAAATTGCCTTAA...............................................................................................................................................................................................................................................................................................................................	19	3	0.67	2	0	1	0	1	0
.....................................................................................................................................................................................................GTAGAAATTGCCTTAATTT............................................................................................................................................................................................................................................................................................................................	19	3	0.67	2	0	1	0	1	0
.............................................................................................................................................................................................TTTTTGATGTAGAAATTGCCTTA................................................................................................................................................................................................................................................................................................................................	23	3	0.67	2	0	1	0	1	0
.................................................................................................................................................................................................TGATGTAGAAATTGCCTTA................................................................................................................................................................................................................................................................................................................................	19	3	0.67	2	2	0	0	0	0
.......................................................TTT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAATTGCCT..................................................................................................................................................................................................................................................................................................................................	29	3	0.67	2	2	0	0	0	0
............................................................................................................................................................................................ATTTTTGATGTAGAAATTGCCC..................................................................................................................................................................................................................................................................................................................................	22	3	0.67	2	2	0	0	0	0
.......................................................TTT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGT.............................................................................................................................................................................................................................................................................................................................................	18	4	0.50	2	0	0	0	2	0
......................................................TTTT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAG...........................................................................................................................................................................................................................................................................................................................................	21	4	0.50	2	0	0	0	2	0
.........................................................................................................................................................................................TTAATTTTTGATGTAGAAAT.......................................................................................................................................................................................................................................................................................................................................	20	4	0.50	2	0	0	0	2	0
........................................................TT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAA.........................................................................................................................................................................................................................................................................................................................................	21	4	0.50	2	0	0	0	2	0
................................................................................................................................................................................................TTGATGTAGAAATTGCCG..................................................................................................................................................................................................................................................................................................................................	18	6	0.50	3	3	0	0	0	0
........................................................................................................................................................................................TTTAATTTTTGATGTAGAA.........................................................................................................................................................................................................................................................................................................................................	19	4	0.50	2	0	0	0	2	0
.......................................................TTT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAATT......................................................................................................................................................................................................................................................................................................................................	25	4	0.50	2	1	0	0	1	0
..............................................................................................................................................................................................TTTTGATGTAGAAATTGCCTTAATTT............................................................................................................................................................................................................................................................................................................................	26	3	0.33	1	0	0	0	1	0
.......................................................TTT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAATTGCCA..................................................................................................................................................................................................................................................................................................................................	29	3	0.33	1	1	0	0	0	0
.............................................................................................................................................................................................TTTTTGATGTAGAAATTGCCTTAATT.............................................................................................................................................................................................................................................................................................................................	26	3	0.33	1	0	0	0	1	0
........................................................TT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAATTGCC...................................................................................................................................................................................................................................................................................................................................	27	3	0.33	1	1	0	0	0	0
...........................................................................................................................................................................................................................................................................................................................................ATTACTTCAATT-----ATTTTTATTTTAATT.........................................................................................................................................................................	27	3	0.33	1	0	1	0	0	0
.....................................................................................................................................................................................................GTAGAAATTGCCTTAATT.............................................................................................................................................................................................................................................................................................................................	18	3	0.33	1	0	0	0	1	0
..........................................................................................................................................................................................TAATTTTTGATGTAGAAATTGCCA..................................................................................................................................................................................................................................................................................................................................	24	3	0.33	1	1	0	0	0	0
.............................................................................................................................................................................................TTTTTGATGTAGAAATTGCCTTAA...............................................................................................................................................................................................................................................................................................................................	24	3	0.33	1	0	0	0	1	0
................................................................................................................................................................................................................................................................................................TTTG--------------------------------AAG---AATTACTTCAATA-----........................................................................................................................................................................................	20	3	0.33	1	0	0	0	1	0
.................................................................................................................................................................................................TGATGTAGAAATTGCCTTAATTTTA..........................................................................................................................................................................................................................................................................................................................	25	3	0.33	1	0	1	0	0	0
..................................................................................................................................................................................................GATGTAGAAATTGCCTTAATAA............................................................................................................................................................................................................................................................................................................................	22	3	0.33	1	0	1	0	0	0
................................................................................................................................................................................................TTGATGTAGAAATTGCCTTAA...............................................................................................................................................................................................................................................................................................................................	21	3	0.33	1	0	0	0	1	0
...........................................................................................................................................................................................AATTTTTGATGTAGAAATTGCCTTACC..............................................................................................................................................................................................................................................................................................................................	27	3	0.33	1	0	0	0	1	0
...................................................................................................................................................................................................ATGTAGAAATTGCCTTAAT..............................................................................................................................................................................................................................................................................................................................	19	3	0.33	1	0	1	0	0	0
............................................................................................................................................................................................ATTTTTGATGTAGAAATTGCCTTAATTT............................................................................................................................................................................................................................................................................................................................	28	3	0.33	1	0	0	0	1	0
..................................................................................................................................................................................................GATGTAGAAATTGCCTTAATTACC..........................................................................................................................................................................................................................................................................................................................	24	3	0.33	1	0	0	0	1	0
........................................................TT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAATTGCCTT.................................................................................................................................................................................................................................................................................................................................	29	3	0.33	1	1	0	0	0	0
...............................................................................................................................................................................................TTTGATGTAGAAATTGCCA..................................................................................................................................................................................................................................................................................................................................	19	3	0.33	1	1	0	0	0	0
...........................................................................................................................................................................................AATTTTTGATGTAGAAATTGCCG..................................................................................................................................................................................................................................................................................................................................	23	3	0.33	1	0	0	0	1	0
....................................................................................................................................................................................................TGTAGAAATTGCCTTAATT.............................................................................................................................................................................................................................................................................................................................	19	3	0.33	1	0	0	0	1	0
..................................................................................................................................................................................................GATGTAGAAATTGCCTTAATTTT...........................................................................................................................................................................................................................................................................................................................	23	3	0.33	1	0	0	0	1	0
.................................................................................................................................................................................................TGATGTAGAAATTGCCTTAATTTTACC........................................................................................................................................................................................................................................................................................................................	27	3	0.33	1	0	0	0	0	1
................................................................................................................................................................................................TTGATGTAGAAATTGCCTTACC..............................................................................................................................................................................................................................................................................................................................	22	3	0.33	1	1	0	0	0	0
...............................................................................................................................................................................................TTTGATGTAGAAATTGCCTTAATTTTAC.........................................................................................................................................................................................................................................................................................................................	28	3	0.33	1	0	1	0	0	0
............................................................................................................................................................................................ATTTTTGATGTAGAAATTGCCTA.................................................................................................................................................................................................................................................................................................................................	23	3	0.33	1	0	0	0	1	0
..................................................................................................................................................................................................GATGTAGAAATTGCCTTAATTTTT..........................................................................................................................................................................................................................................................................................................................	24	3	0.33	1	0	0	0	0	1
...........................................................................................................................................................................................AATTTTTGATGTAGAAATTGCCTTAATT.............................................................................................................................................................................................................................................................................................................................	28	3	0.33	1	0	0	0	1	0
................................................................................................................................................................................................................................................................................................................................................TTCAATT-----ATTTTTATTTTAATT.........................................................................................................................................................................	22	3	0.33	1	0	1	0	0	0
.........................................................................................................................................................................................TTAATTTTTGATGTAGAAATTGCCG..................................................................................................................................................................................................................................................................................................................................	25	3	0.33	1	1	0	0	0	0
.............................................................................................................................................................................................TTTTTGATGTAGAAATTGCCTC.................................................................................................................................................................................................................................................................................................................................	22	3	0.33	1	1	0	0	0	0
...........................................................................................................................................................................................AATTTTTGATGTAGAAATTGCCTA.................................................................................................................................................................................................................................................................................................................................	24	3	0.33	1	0	0	0	1	0
...............................................................................................................................................................................................................................................................................................ATTTG--------------------------------AAG---AATTACTTCA................................................................................................................................................................................................	18	3	0.33	1	0	0	0	1	0
..................................................................................................................................................................................................GATGTAGAAATTGCCTTAATTTTAC.........................................................................................................................................................................................................................................................................................................................	25	3	0.33	1	0	0	0	0	1
...................................................................................................................................................................................................ATGTAGAAATTGCCTTAATT.............................................................................................................................................................................................................................................................................................................................	20	3	0.33	1	0	0	0	1	0
...........................................................................................................................................................................................AATTTTTGATGTAGAAATTGCA...................................................................................................................................................................................................................................................................................................................................	22	4	0.25	1	0	0	0	1	0
......................................................TTTT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAA.........................................................................................................................................................................................................................................................................................................................................	23	4	0.25	1	0	0	0	1	0
.......................................................TTT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAA........................................................................................................................................................................................................................................................................................................................................	23	4	0.25	1	0	0	0	1	0
........................................................................................................................................................................................TTTAATTTTTGATGTAGAAATT......................................................................................................................................................................................................................................................................................................................................	22	4	0.25	1	1	0	0	0	0
.....................................................................................................................................................................................................................................................................................................................................................................................................................................ATAC-----------------------------------CATGA------------ATGAAATCAA---G-----------------------------------------	20	4	0.25	1	0	0	0	1	0
.........................................................T------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAT........................................................................................................................................................................................................................................................................................................................................	21	4	0.25	1	0	0	0	1	0
..........................................................................................................................................................................................TAATTTTTGATGTAGAAAA.......................................................................................................................................................................................................................................................................................................................................	19	4	0.25	1	0	0	0	1	0
........................................................................................................................................................................................TTTAATTTTTGATGTAGAAA........................................................................................................................................................................................................................................................................................................................................	20	4	0.25	1	0	0	0	1	0
........................................................TT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAA........................................................................................................................................................................................................................................................................................................................................	22	4	0.25	1	0	0	0	1	0
.......................................................TTT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAATTGC....................................................................................................................................................................................................................................................................................................................................	27	4	0.25	1	0	0	0	1	0
....................................................................................................................................................................................................................................................................................................................................................................................................................................TATAC-----------------------------------CATGA------------ATGAAATC...............................................	18	4	0.25	1	0	0	0	1	0
.........................................................................................................................................................................................TTAATTTTTGATGTAGAA.........................................................................................................................................................................................................................................................................................................................................	18	4	0.25	1	0	0	0	1	0
......................................................TTTT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAAATT......................................................................................................................................................................................................................................................................................................................................	26	4	0.25	1	1	0	0	0	0
.........................................................T------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATGTAGAG.........................................................................................................................................................................................................................................................................................................................................	20	4	0.25	1	0	0	0	1	0
............................................................................................................................................................................................ATTTTTGATGTAGAAATTG.....................................................................................................................................................................................................................................................................................................................................	19	4	0.25	1	0	1	0	0	0
......................................................TTTT------------------------------------------------------------------------------------------------------------------------------TTTAATTTTTGATG..............................................................................................................................................................................................................................................................................................................................................	18	5	0.20	1	0	0	0	1	0
............................................................................................................................................................................................ATTTTTGATGTAGAAATTGCA...................................................................................................................................................................................................................................................................................................................................	21	5	0.20	1	0	0	0	1	0
..........................................................................................................................................................................................TAATTTTTGATGTAGAAAGC......................................................................................................................................................................................................................................................................................................................................	20	5	0.20	1	0	0	0	1	0
...........................................................................................................................................................................................AATTTTTGATGTAGAAAA.......................................................................................................................................................................................................................................................................................................................................	18	5	0.20	1	0	0	0	1	0
............................................................................................................................................................................................................................................................................................................................................................ATTTTTATTTTAATTTTATT....................................................................................................................................................................	20	6	0.17	1	0	0	0	1	0
...........................................................................................................................................................................................AATTTTTGATGTAGAAAC.......................................................................................................................................................................................................................................................................................................................................	18	9	0.11	1	0	0	0	1	0
...........................................................................................................................................................................................AATTTTTGATGTAGAAAGC......................................................................................................................................................................................................................................................................................................................................	19	11	0.09	1	0	0	0	1	0
...................................................................................................................................................................................................................................................................................................................................................AATT-----ATTTTTATTTTAAT..........................................................................................................................................................................	18	12	0.08	1	0	0	0	1	0

dp5

CGTG---ATTGTTGCCGGT---------------GGCGGTGTTGTGGCCAATGGCCCA--ACCACGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
...G---ATTGTTGCCGGT---------------GGCGGTG...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0

droPer2

CGTG---ATTGTTGCCGGT---------------GGCGGAGTTGTGGCCAATGGCCCA--ACCACGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
No Reads

droAna3

CGTG---ATTGTTTCCGGG---------------GGCGT------GGCCAATGCGCCG--ACCTCGAATTTAAACAGTAAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
No Reads

droBip1

CGTG---ATTGTTTCCGGG---------------GGCGT------GGCCAATGGGCCG--ACCTCGAATTTAAACAGTAAGTA-------------------------TTTC---GGAT------------------------------------------------------------GCATATCTAATA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CACTCACTTAAAATTACAC-----------------------------------TCTGG------------ATAAAAGCCA---AATTTATAAATGAATTTGTTTAATAAATATTTTGAGCTATTT	Size	Hit Count	Total Norm	Total
No Reads

droKik1

CCTG---ATTGTTGCCGGC---------------GGCGT------GGCCAATGTGCCG--ACCTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droFic1

CGTG---ATTGTGGCCGGA---------------GGCGG------GGCCAATGTGCCG--ACCTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEle1

CGTG---ATTGTGGCCGGA---------------GGCGG------GGCCAATGTGCCG--ACCTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droRho1

CGTG---ATTGTGGCCGGA---------------GGCGG------GGCCAATGTGCCG--ACCTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droBia1

CGTG---ATTGTGGCCGGA---------------GGCGG------GGCCAATGTGCCG--ACTTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droTak1

CGTG---ATTGTGGCCGGA---------------GGCGG------GGCCAATGTGCCG--ACTTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEug1

TGTCTACATTATCGCCAAATAAAACAAATAAATCCGCGA------CACCTAACTGTGGGAACATTA-------------AGAATTCCCTCTCTTTGCAATTTTGAGTAAAATCG----------ACTT----TTCT------------------------------------------------CGAAAATAAAAACTTATGAATTAAATAAATTTT--------TTTTTATTTTAAT-TTTTCTTATTTCTCTCGAATATAAAAAACCTTTGAAAAAAAAAAATTTACTTA--------------------------------AACAATACTTAAATTAAGTAAAAAGCTCTTATTTGAATTTTTCTGGGTGTTCCAAAGAGAACATATTAAAACAC---AAATATATTTAT-----------------------------------------------CTAGC------------AGATTCTAAAATAA-----------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

dm3

CGTG---ATTGTGGCCGGA---------------GGCGG------GGCCAATGTGCCG--ACCTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	GSM154618 Ovary	GSM154620 Ovary	GSM154621 Ovary	GSM154622 Ovary	GSM180328 Head	GSM180329 Mixed-body	GSM180330 Embryo	GSM180331 Embryo	GSM180332 Embryo	GSM180333 Embryo	GSM180334 Larvae	GSM180335 Disc	GSM180336 Pupae	GSM180337 S2	GSM231091 Ovary	GSM239041 Head	GSM239050 Head	GSM239051 S2	GSM239052 S2	GSM246084 Head	GSM266765 S2	GSM280082 Ovary	GSM280083 Ovary	GSM280084 Ovary	GSM280085 Testis	GSM280086 Ovary	GSM280087 S2-NP	GSM280088 S2-NP	GSM312995 Head	GSM313160 Head	GSM313161 Head	GSM313162 Head	GSM313163 Head	GSM313164 Head	GSM313165 Head	GSM343287 Embryo	GSM379050 Ovary	GSM379051 Ovary	GSM379052 Ovary	GSM379053 Ovary	GSM379054 Ovary	GSM379055 Ovary	GSM379056 Ovary	GSM379057 Ovary	GSM379058 Ovary	GSM379059 Ovary	GSM379060 Ovary	GSM379061 Ovary	GSM379062 Ovary	GSM379063 Ovary	GSM379064 Ovary	GSM379065 Ovary	GSM379066 Ovary	GSM379067 Ovary	GSM424739 S2	GSM424740 S2	GSM424741 S2	GSM467729 Female-body	GSM467730 Female-body	GSM467731 Female-body	M002 CS	M003 Male-body	M004 Male-body	M005 ML-DmD32	M006 ML-DmD32	M029 OSC	M030 OSC	M031 OSC	M032 S2-R+	M033 S2-R+	M034 S2-R+	M035 S2-R+	M036 S2-R+	M037 S2-R+	M038 S2-R+	M039 S2-R+	M048 S2-R+	OSS2 OSS	OSS6 OSS	OSS7 OSS	OSS8 OSS	S1 Head	S10 Embryo	S11 Embryo	S12 Embryo	S13 Embryo	S14 Embryo	S15 S2-GMR	S16 Kc	S17 Larvae	S18 Larvae	S19 Pupae	S2 Head	S20 Pupae	S21 Disc	S22 Head	S23 Female-body	S24 Male-body	S25 Larvae	S26 Larvae	S27 Pupae	S28 Pupae	S29 Head	S3 Male-body	S30 S2-GMR	S31 S2	S32 S2	S33 Kc	S34 Kc	S35 Kc	S4 Female-body	S5 Disc	S6 Embryo	S7 Embryo	S8 S2	S9 Embryo	SRR001337 Head	SRR001338 Head	SRR001339 Head	SRR001340 Head	SRR001341 Head	SRR001342 Head	SRR001343 Head	SRR001344 Head	SRR001345 Head	SRR001346 Head	SRR001347 Head	SRR001348 Head	SRR001349 Head	SRR001664 Head	SRR010951 Ovary	SRR010952 Ovary	SRR010953 Ovary	SRR010954 Ovary	SRR010955 Ovary	SRR010956 Ovary	SRR010957 Ovary	SRR010958 Ovary	SRR010959 Ovary	SRR010960 Ovary	SRR014268 Embryo	SRR014269 Embryo	SRR014270 Embryo	SRR014271 Embryo	SRR014272 Embryo	SRR014273 Ovary	SRR014274 Ovary	SRR014275 Ovary	SRR014276 Ovary	SRR014277 Ovary	SRR014278 Ovary	SRR014279 Ovary	SRR014280 Ovary	SRR014281 Ovary	SRR014282 Ovary	SRR023197 S2	SRR023399 S2	SRR023400 S2	SRR023402 S2	SRR023407 S2	SRR029028 S2	SRR029029 S2	SRR029030 S2	SRR029031 S2	SRR029032 S2	SRR029033 S2	SRR029608 Head	SRR029633 Head	SRR030145 gb3	SRR031692 Head	SRR031693 Head	SRR031694 Head	SRR031696 Head	SRR031697 Head	SRR031698 Head	SRR031699 Head	SRR031700 Head	SRR031701 Head	SRR031702 Head	SRR031703 Head	SRR031704 Head	SRR032092 S2	SRR032093 S2	SRR032094 S2	SRR032095 S2-NP	SRR032096 S2-NP	SRR032152 Head	SRR060643 Testis	SRR060644 Ovary	SRR060645 Testis	SRR060646 Ovary	SRR060647 Ovary	SRR060648 Ovary	SRR060649 Ovary	SRR060650 Testis	SRR060651 Ovary	SRR060652 Testis	SRR060653 Ovary	SRR065151 Ovary	SRR065152 Ovary	SRR065153 Ovary	SRR065154 Ovary	SRR065155 Ovary	SRR065800 Ovary	SRR065801 Ovary	SRR065802 Ovary	SRR065803 Ovary	SRR065804 Ovary	SRR065805 Ovary	SRR065806 Ovary	SRR065807 Ovary	SRR097865 S2-NP	SRR097866 S2-NP	SRR097867 S2-NP	SRR298536 Ovary	SRR298537 Ovary	SRR298711 Ovary	SRR298712 Ovary	SRR317110 Ovary	SRR317111 Ovary	SRR317112 Ovary	SRR317113 Ovary	SRR317114 Ovary	SRR317115 Ovary	SRR317116 Ovary	SRR317117 Ovary	SRR341115 S2	SRR341116 S2	SRR341117 S2	SRR341118 S2	SRR351332 Mixed-body	SRR351333 Mixed-body	T004 OSC	T005 CME-L1	V001 Embryo	V002 Embryo	V003 S2	V006 Female-body	V007 Head	V008 S2	V009 CME-L1	V010 ML-DmD20-C5	V011 Sg4	V012 Male-body	V013 Head	V014 DTT	V015 DreRFHV	V018 Kc167	V019 GM2	V020 S2-R+	V021 ML-DmD21	V022 ML-DmD32	V023 Head	V024 Kc167	V025 S2-R+	V026 1182-2H	V027 ML-DmD21	V028 CME-W1-Cl.8+	V029 ML-DmD9	V030 ML-DmD8	V031 GM2	V032 S1	V033 CME-W1-Cl.8+	V034 ML-DmD16-C3	V035 ML-DmD32	V036 ML-DmD20-C5	V037 S2	V038 S2	V063 Ovary	V0632 Ovary	V064 Ovary	V0642 Ovary	V065 Ovary	V0652 Ovary	V066 Ovary	V0662 Ovary	V067 S2-NP	V073 mbn2	V074 S3	V075 ML-DmBG1-C1	V076 ML-DmBG3-C2	V077 Head	V078 Head	V079 Head	V080 Head	V081 Embryo	V082 Embryo	V083 Male-body	V084 Female-body	V085 CME-W2	V086 Female-body	V088 Ovary	V0882 Ovary	V089 Ovary	V0892 Ovary	V090 Male-body	V091 fGS/OSS	V092 Male-body	V093 Male-body	V094 S2-R+	V095 S2-R+	V096 Ovary	V097 Ovary	V098 Ovary	V125 ML-DmD9	V126 CME-L1	V127 G2	V128 S3	V129 ML-DmBG1-C1	V130 ML-DmBG3-C2	V131 ML-DmD16-C3	V132 ML-DmD32	V133 Sg4	V134 ML-DmD8	V135 CME-W2	V136 Male-body	V137 Head	V138 Male-body	V139 Female-body	V140 Female-body	V141 Female-body	V142 Female-body	V144 OSC	V145 S2	V146 S1	V147 1182-2H	V148 mbn2	GSM1528798 follicle cells
............GGCCGGA---------------GGCGG------GGCCAAC................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droSim2

CGTG---ATTGTGGCCGGA---------------GGCGG------GGCCAATGTGCCG--ACCTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Size

Hit Count

Total Norm

Total

M023
Head

M024
Male-body

M025
Embryo

M053
Female-body

O001
Testis

O002
Head

SRR553485
Ovary

SRR553486
Ovary

SRR553487
Ovary

SRR553488
Ovary

SRR618934
Ovary

V044
Embryo

SRR902008
Ovary

SRR902009
Testis

SRR1275487
Male larvae

SRR1275485
Male prepupae

SRR1275483
Male prepupae

No Reads

droSec2

CGTG---ATTGTGGCCGGA---------------GGCGG------GGCCAATGTGCCG--ACCTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
No Reads

droYak3

Size

Hit Count

Total Norm

Total

M026
Head

M043
Female-body

M056
Embryo

V046
Embryo

V052
Head

V058
Head

V120
Male-body

SRR1275488
Male larvae

SRR1275486
Male prepupae

SRR1275484
Male prepupae

GSM1528802
follicle cells

No Reads

droEre2

CGTG---ATTGTGGCCGGC---------------GGCGG------GGCCAATGTGCCG--ACCTCGAATTTAAACAGTGAGTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
No Reads

ACATTAACAGCGGCCGCCATACGGGTTACACGGTTGTAGCTTAAATTTATCATTCATCTTGGCGGGTGTTTTGCGCGATAAAGTCTAATGTTTAACGTTTACAAATAAATGTAAACAAAAAGTCTAAAAATCATTCGAAAAACACCTCTTTTGTATAAGTTAATATATTATTTTTCGAGTTAATATAATAATAACGAAGTTATGCTTTAACAAATCTATCTAAATTACATAAATAAATCTTTTTTAACTTT ************************************************..((((((......((((((((.((..((((..(((((..(((..((((((((......)))))))).)))..)))))..)))).)).)))))))).(((......)))(((.(((((((..........))))))).))).))))))...************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR060660 Argentina_ovaries_total	SRR060679 140x9_testes_total	SRR060659 Argentina_testes_total	SRR060657 140_testes_total	SRR060681 Argx9_testes_total	SRR060682 9x140_0-2h_embryos_total	SRR060658 140_ovaries_total	SRR060672 9x160_females_carcasses_total	SRR060668 160x9_males_carcasses_total	SRR060664 9_males_carcasses_total	SRR060666 160_males_carcasses_total	V116 male body	SRR060680 9xArg_testes_total	SRR060686 Argx9_0-2h_embryos_total	V047 embryo	SRR060667 160_females_carcasses_total	SRR060678 9x140_testes_total	SRR1106729 mixed whole adult body	SRR060674 9x140_ovaries_total	SRR060687 9_0-2h_embryos_total	SRR1106726 embryo_14-16h	SRR060683 160_testes_total	V053 head	SRR060675 140x9_ovaries_total	SRR060676 9xArg_ovaries_total	SRR060685 9xArg_0-2h_embryos_total
.....................................AGCTTAAATTTGGCATTC....................................................................................................................................................................................................	18	2	4	7.50	30	8	1	6	6	2	0	2	0	0	0	0	2	1	1	0	1	0	0	0	0	0	0	0	0	0	0
..................................TGTAGCTTAAATTTGGCATGC....................................................................................................................................................................................................	21	3	2	4.00	8	0	2	0	0	0	2	0	1	1	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................AGCTTAAATTTAGCATGC....................................................................................................................................................................................................	18	2	7	0.71	5	0	1	0	0	0	0	0	1	0	0	0	0	1	0	0	0	0	0	1	1	0	0	0	0	0	0
.....................................AGCTTCAATTTAGCATGC....................................................................................................................................................................................................	18	3	20	0.70	14	1	3	0	0	5	0	0	0	0	0	0	0	0	1	0	0	1	0	0	0	0	0	0	1	1	1
...................................GTAGCTTAAATTTGGCATGC....................................................................................................................................................................................................	20	3	4	0.50	2	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................AGCTTAAATTTGTCATGC....................................................................................................................................................................................................	18	2	5	0.40	2	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................TCATTTTTCGAGTTAAGAT.................................................................	19	2	8	0.25	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0	0	0	0	0
.....................................AGCTCAAATTTAGCATTC....................................................................................................................................................................................................	18	2	8	0.13	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................TTTCGAGTTAATATAGTAA............................................................	19	1	10	0.10	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0
.............................................................................................................................................................................TTCGAGTTAATATAGTAAT...........................................................	19	1	10	0.10	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0
............................................................................................................................................................................TTTCGAGTTAAGATAATA.............................................................	18	1	12	0.08	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
..............................................................................................................................................................................TCGAGTTAATATAGTAAAA..........................................................	19	2	14	0.07	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0
........TGCGGCCGCCATACAGGTA................................................................................................................................................................................................................................	19	3	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................................TCGAGTTAATATAGTAAG...........................................................	18	2	20	0.05	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................CAGCTTAAATTTAGCATGC....................................................................................................................................................................................................	19	3	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................TCATTTTTCGAGTTAA....................................................................	16	1	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0
..................................TGTAGCTCAAATTTGGCAT......................................................................................................................................................................................................	19	3	20	0.05	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

dvi_9866

scaffold_12963:393981-394131 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads