dvi_9742

GTTATGATGGCCTACGTCCAGCCTTAACTCCTTCACGCGCAGTGGCTTTGCACTCTCGCCTTGCTTTGCCTTGCCATAACTCGTCTTTGACACTTGTCAATTTTCACGACATTTATCTTTAAGCTTATTAATCATTTGTCAACCCCCATAGAAGAGAACTTAGACCGTTGCGAAAAACCTCCGTCTCAACCAAGTTCTTTAACAAGTACTTATATGCCGTTGCGGAGAACCGCAAGTTGTTGCGAAGAGTA ************************************************.........(((.((.((..(((..((((((...(((.((((..(((((.......)))))..))))...)))......))))))....)))....)).)))))(((((((((.(((...(((((...)))))...))))))))))))...************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M028 head	SRR060674 9x140_ovaries_total	M027 male body	M061 embryo	SRR060679 140x9_testes_total	M047 female body	SRR060687 9_0-2h_embryos_total	SRR060654 160x9_ovaries_total	SRR1106723 embryo_12-14h	SRR060662 9x160_0-2h_embryos_total	SRR060682 9x140_0-2h_embryos_total	SRR060684 140x9_0-2h_embryos_total	SRR060686 Argx9_0-2h_embryos_total	SRR1106729 mixed whole adult body
................TCCAGCCTTAACCCCTTCACGC.....................................................................................................................................................................................................................	22	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................TCAACAAAGTTCTTAAACATGTA...........................................	23	3	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................CCCGTCTCAACCAAGTTCTTTAA.................................................	23	1	2	0.50	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................................................................................................CCGTCTCAACCAAGTTCTTTA..................................................	21	0	2	0.50	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................GAACCGCAAGTTGAGGCAAAG....	21	3	3	0.33	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0
....TGATGGCCTACGTCCAGCCTTAAC...............................................................................................................................................................................................................................	24	0	13	0.23	3	0	0	0	0	0	2	0	0	1	0	0	0	0	0
.....................................................................................................................................................................................................................................CCGCAAGTTGAGGCAAAGA...	19	3	15	0.13	2	0	0	1	0	0	0	0	0	0	1	0	0	0	0
....................................................................................................................................CACTTGAGAACCCCCATAG....................................................................................................	19	3	9	0.11	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0
....TGATGGCCTACGTCCAGCCTT..................................................................................................................................................................................................................................	21	0	13	0.08	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0
..GAAGATGGCCTACGTCCAGCCTTAACC..............................................................................................................................................................................................................................	27	3	14	0.07	1	0	0	0	0	0	1	0	0	0	0	0	0	0	0
...................................................................................................................................TCATTTGAGAACCACCATA.....................................................................................................	19	3	20	0.05	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0
....................................................................CATTGCCAAAAGTCGTCTT....................................................................................................................................................................	19	3	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0
.......................................................................................................................................................................................TCGCAACGAAGTTCTCTAA.................................................	19	3	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0
.............ACGTCCAGCCTTGAC...............................................................................................................................................................................................................................	15	1	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droVir3	scaffold_12958:1907027-1907277 +	dvi_9742	CAATACTACCGGATGCAGGTCGGAATTGAGGAAGTGCGCGTCACCGAAACGTGAGAGCGGAACGAAACGGAACGGTATTGAGCAGAAACTGTGAACAGTTAAAAGTGCTGTAAATAGAAATTCGAATAATTAGTAAACAGTTGGGGGTATCTTCTCTTGAATCTGGCAACGCTTTTTGGAGGCAGAGTTGGTTCAAGAAATTGTTCATGAATATACGGCAACGCCTCTTGGCGTTCAACAACGCTTCTCAT
droMoj3	scaffold_6328:3899343-3899420 +		ATCTTC----------------------------------------------------------------------------------------------------------------------------------------------------ACTTGAATATGGCAACGCTTCTTGGCTTACAAATTGCC-----AAATTGCCATTAGAT--CTGGCAGCGCTTCTAGGCG------------------
droGri2	scaffold_15116:785129-785177 -		CCAAAATTTGGGATGCAAATGGGACTTGGAAATGCGGTAGTAACGGA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AA------------------
droWil2	scf2_1100000004900:42123-42147 -		ACCAATTGTGGGCCGAAGCCAG------------------------G----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AA------------------
dp5	Unknown_group_334:4297-4345 +		CCCAATTACCGTAGCCAAGTCGGGATCGGTCAGGAAGTGGTCACTGA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GA------------------
droPer2	scaffold_108:32554-32600 +		CAGAACTAGCGGATGCAGGTCGGAATAGGGCAATAGGTGGTCACCG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------A------------------
droAna3	scaffold_958:1206-1211 -		CCAAAC-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droBip1	scf7180000395610:3464-3495 +		CATTACGA--GACCGCCGATGGGAA------AAGAAGT-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AG------------------
droKik1	scf7180000301114:6476-6542 +		CCAAACCACCGGATGATGGTGGGGATCGGAGAGCGGGTACAGACGGA------------------------GCGGCCTT-TCCAGAAGCT---------------------------------------------------------------------------------------------------------------------------------------------GT------------------
droFic1	scf7180000453853:22169-22217 +		CCAAATTTCCGGATGCAGGTTGGCATCGGGCAGATGCTAGAGACGGA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AA------------------
droEle1	scf7180000489220:756-803 +		CCAAATTATCGTTGTCAAGTTGGAATTGGTCAAGAAGTAGTCACGG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AA------------------
droRho1	scf7180000777398:2489-2525 -		CCAAACTTCCGGATGCAGGTCGGTATTGGAGAAACA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------C------------------
droBia1	scf7180000301521:27701-27735 -		CCAAACTTCAGGATGCAGGTCCGAATTGGAAG--------------A----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AG------------------
droTak1	scf7180000415248:589760-589841 -		AAGTTAGGTTAAGTACA-----------------GTGTGGTTAATAA----------------------------------------------------------------------------------------------------TATCTTCTCTTGTGTTTGGCGTAGCTTCTTGGCTGTGGCCTCAGTTGGGG----------------------------------AA------------------
droEug1	scf7180000409459:34748-34775 -		CAACCC----GGATT-----------------------------------------------------------ACCAG-GCCAAAAATG---------------------------------------------------------------------------------------------------------------------------------------------GG------------------
droYak3	v2_chrUn_4989:180529-180577 -		CCAAATTACCGCACACAGGTCGGGATAGGGCAGGAAGTGGTGACTGA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AA------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droVir3

CAATACTACCGGATGCAGGTCGGAATTGAGGAAGTGCGCGTCACCGAAACGTGAGAGCGGAACGAAACGGAACGGTATTGAGCAGAAACTGTGAACAGTTAAAAGTGCTGTAAATAGAAATTCGAATAATTAGTAAACAGTTGGGGGTATCTTCTCTTGAATCTGGCAACGCTTTTTGGAGGCAGAGTTGGTTCAAGAAATTGTTCATGAATATACGGCAACGCCTCTTGGCGTTCAACAACGCTTCTCAT	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
......TACCGGATGCAGGTCGGAATTG...............................................................................................................................................................................................................................	22	13	0.38	5	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....ACTACCGGATGCAGGTCGGCG..................................................................................................................................................................................................................................	21	13	0.08	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droMoj3

ATCTTC----------------------------------------------------------------------------------------------------------------------------------------------------ACTTGAATATGGCAACGCTTCTTGGCTTACAAATTGCC-----AAATTGCCATTAGAT--CTGGCAGCGCTTCTAGGCG------------------	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
No Reads

droGri2

CCAAAATTTGGGATGCAAATGGGACTTGGAAATGCGGTAGTAACGGA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AA------------------	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

droWil2

ACCAATTGTGGGCCGAAGCCAG------------------------G----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AA------------------	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
No Reads

dp5

CCCAATTACCGTAGCCAAGTCGGGATCGGTCAGGAAGTGGTCACTGA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GA------------------	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
.........................TCGGTCAGGAAGTGGTCACTGA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------....................	22	1	13.00	13	0	7	0	0	0	0	0	6	0	0
.........................TCGGTCAGGAAGTGGTCACTGA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GA------------------	24	1	10.00	10	0	9	0	0	0	0	0	1	0	0
.........................TCGGTCAGGAAGTGGTCACTG.............................................................................................................................................................................................................	21	1	9.00	9	0	9	0	0	0	0	0	0	0	0
..........................CGGTCAGGAAGTGGTCACTGA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GA------------------	23	1	7.00	7	0	4	0	0	0	0	0	3	0	0
..........................CGGTCAGGAAGTGGTCACTGA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------G...................	22	1	4.00	4	0	4	0	0	0	0	0	0	0	0
.........................TCGGTCAGGAAGTGGTCACT..............................................................................................................................................................................................................	20	1	4.00	4	0	4	0	0	0	0	0	0	0	0
.........................TCGGTCAGGAAGTGGTCACTGA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------G...................	23	1	2.00	2	0	1	0	0	0	0	0	1	0	0
..........................CGGTCAGGAAGTGGTCACTG.............................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..........................CGGTCAGGAAGTGGTCACTGA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------....................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0
........................ATCGGTCAGGAAGTGGTCACTG.............................................................................................................................................................................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.........................TCGGTCAGGAAGTGGTCAC...............................................................................................................................................................................................................	19	3	0.67	2	0	2	0	0	0	0	0	0	0	0
...........TAGCCAAGTCGGGATCGGTCAGGAAA......................................................................................................................................................................................................................	26	3	0.67	2	0	2	0	0	0	0	0	0	0	0
...........TAGCCAAGTCGGGATCGGTCAGGA........................................................................................................................................................................................................................	24	3	0.67	2	0	0	0	0	0	0	0	2	0	0
...............CAAGTCGGGATCGGTCAGGAAGTGG...................................................................................................................................................................................................................	25	3	0.67	2	0	0	0	0	0	0	0	2	0	0
..................GTCGGGATCGGTCAGGAAGTGGTC.................................................................................................................................................................................................................	24	3	0.33	1	0	1	0	0	0	0	0	0	0	0
...........TAGCCAAGTCGGGATCGGTCAGGAA.......................................................................................................................................................................................................................	25	3	0.33	1	0	1	0	0	0	0	0	0	0	0
.........................TCGGTCAGGAAGTGGTCA................................................................................................................................................................................................................	18	3	0.33	1	0	1	0	0	0	0	0	0	0	0
.................AGTCGGGATCGGTCAGGAAA......................................................................................................................................................................................................................	20	3	0.33	1	0	0	0	0	0	0	0	1	0	0
...........TAGCCAAGTCGGGATCGGTCAGG.........................................................................................................................................................................................................................	23	3	0.33	1	0	1	0	0	0	0	0	0	0	0
....................CGGGATCGGTCAGGAAGTGGTCAC...............................................................................................................................................................................................................	24	3	0.33	1	0	1	0	0	0	0	0	0	0	0

droPer2

CAGAACTAGCGGATGCAGGTCGGAATAGGGCAATAGGTGGTCACCG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------A------------------	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
No Reads

droAna3

CCAAAC-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
No Reads

droBip1

CATTACGA--GACCGCCGATGGGAA------AAGAAGT-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AG------------------	Size	Hit Count	Total Norm	Total
No Reads

droKik1

CCAAACCACCGGATGATGGTGGGGATCGGAGAGCGGGTACAGACGGA------------------------GCGGCCTT-TCCAGAAGCT---------------------------------------------------------------------------------------------------------------------------------------------GT------------------	Size	Hit Count	Total Norm	Total
No Reads

droFic1

CCAAATTTCCGGATGCAGGTTGGCATCGGGCAGATGCTAGAGACGGA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AA------------------	Size	Hit Count	Total Norm	Total
No Reads

droEle1

CCAAATTATCGTTGTCAAGTTGGAATTGGTCAAGAAGTAGTCACGG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AA------------------	Size	Hit Count	Total Norm	Total
No Reads

droRho1

CCAAACTTCCGGATGCAGGTCGGTATTGGAGAAACA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------C------------------	Size	Hit Count	Total Norm	Total
No Reads

droBia1

CCAAACTTCAGGATGCAGGTCCGAATTGGAAG--------------A----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AG------------------	Size	Hit Count	Total Norm	Total
No Reads

droTak1

AAGTTAGGTTAAGTACA-----------------GTGTGGTTAATAA----------------------------------------------------------------------------------------------------TATCTTCTCTTGTGTTTGGCGTAGCTTCTTGGCTGTGGCCTCAGTTGGGG----------------------------------AA------------------	Size	Hit Count	Total Norm	Total
No Reads

droEug1

CAACCC----GGATT-----------------------------------------------------------ACCAG-GCCAAAAATG---------------------------------------------------------------------------------------------------------------------------------------------GG------------------	Size	Hit Count	Total Norm	Total
No Reads

droYak3

CCAAATTACCGCACACAGGTCGGGATAGGGCAGGAAGTGGTGACTGA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AA------------------	Size	Hit Count	Total Norm	Total	M026 Head	M043 Female-body	M056 Embryo	V046 Embryo	V052 Head	V058 Head	V120 Male-body	SRR1275488 Male larvae	SRR1275486 Male prepupae	SRR1275484 Male prepupae	GSM1528802 follicle cells
.........CGCACACAGGTCGGGATAGGGC............................................................................................................................................................................................................................	22	5	3.20	16	0	12	1	1	0	0	2	0	0	0	0
.........CGCACACAGGTCGGGATAGGG.............................................................................................................................................................................................................................	21	5	1.40	7	0	7	0	0	0	0	0	0	0	0	0
.........CGCACACAGGTCGGGATAGGGCA...........................................................................................................................................................................................................................	23	5	1.00	5	0	3	0	0	0	0	2	0	0	0	0
......TACCGCACACAGGTCGGGATAGGGC............................................................................................................................................................................................................................	25	5	0.60	3	0	1	1	0	0	0	1	0	0	0	0
.......ACCGCACACAGGTCGGGATAGGGC............................................................................................................................................................................................................................	24	5	0.40	2	0	2	0	0	0	0	0	0	0	0	0
..........GCACACAGGTCGGGATAGGG.............................................................................................................................................................................................................................	20	5	0.40	2	0	2	0	0	0	0	0	0	0	0	0
.........CGCACACAGGTCGGGATAGGGCAGGA........................................................................................................................................................................................................................	26	5	0.20	1	0	1	0	0	0	0	0	0	0	0	0
...........CACACAGGTCGGGATAGGGCAGGA........................................................................................................................................................................................................................	24	5	0.20	1	0	1	0	0	0	0	0	0	0	0	0
...........CACACAGGTCGGGATAGGG.............................................................................................................................................................................................................................	19	5	0.20	1	0	1	0	0	0	0	0	0	0	0	0
..........GCACACAGGTCGGGATAGGGC............................................................................................................................................................................................................................	21	5	0.20	1	0	1	0	0	0	0	0	0	0	0	0
...........CACACAGGTCGGGATAGGGC............................................................................................................................................................................................................................	20	5	0.20	1	0	0	0	0	0	0	1	0	0	0	0
.....TTACCGCACACAGGTCGGGATAGG..............................................................................................................................................................................................................................	24	5	0.20	1	0	1	0	0	0	0	0	0	0	0	0
CCAAATTACCGCACACAGGTCGGG...................................................................................................................................................................................................................................	24	5	0.20	1	0	1	0	0	0	0	0	0	0	0	0
......TACCGCACACAGGTCGGGATAGGGCA...........................................................................................................................................................................................................................	26	5	0.20	1	0	1	0	0	0	0	0	0	0	0	0
.......ACCGCACACAGGTCGGGATAGGA.............................................................................................................................................................................................................................	23	5	0.20	1	0	1	0	0	0	0	0	0	0	0	0
.......ACCGCACACAGGTCGGGATAGGG.............................................................................................................................................................................................................................	23	5	0.20	1	0	1	0	0	0	0	0	0	0	0	0
...........CACACAGGTCGGGATAGGGCA...........................................................................................................................................................................................................................	21	5	0.20	1	0	0	0	0	0	0	1	0	0	0	0
...................TCGGGATAGGGCAGGAAGTG....................................................................................................................................................................................................................	20	6	0.17	1	0	1	0	0	0	0	0	0	0	0	0
...................TCGGGATAGGGCAGGAAGTGGTGAC...............................................................................................................................................................................................................	25	6	0.17	1	0	1	0	0	0	0	0	0	0	0	0

dvi_9742

scaffold_12958:1907077-1907227 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

##################################################--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CAATACTACCGGATGCAGGTCGGAATTGAGGAAGTGCGCGTCACCGAAACGTGAGAGCGGAACGAAACGGAACGGTATTGAGCAGAAACTGTGAACAGTTAAAAGTGCTGTAAATAGAAATTCGAATAATTAGTAAACAGTTGGGGGTATCTTCTCTTGAATCTGGCAACGCTTTTTGGAGGCAGAGTTGGTTCAAGAAATTGTTCATGAATATACGGCAACGCCTCTTGGCGTTCAACAACGCTTCTCAT ************************************************.........(((.((.((..(((..((((((...(((.((((..(((((.......)))))..))))...)))......))))))....)))....)).)))))(((((((((.(((...(((((...)))))...))))))))))))...************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR060658 140_ovaries_total	M047 female body	SRR060656 9x160_ovaries_total	SRR060662 9x160_0-2h_embryos_total	V053 head	SRR1106729 mixed whole adult body	SRR060672 9x160_females_carcasses_total	V116 male body	SRR060654 160x9_ovaries_total	SRR060673 9_ovaries_total	SRR060674 9x140_ovaries_total	SRR060675 140x9_ovaries_total	SRR060688 160_ovaries_total	M027 male body	SRR060676 9xArg_ovaries_total	SRR060678 9x140_testes_total	SRR060689 160x9_testes_total
............................................................................................................................................................................TTTTCGGACCCAGAGTTGGT...........................................................	20	3	2	1.50	3	2	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
.............TGCAGGTCGGAATTGAAAAA..........................................................................................................................................................................................................................	20	2	3	0.67	2	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................TGAACAGCTGGGGGTATC....................................................................................................	18	2	2	0.50	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......TACCGGATGCAGGTCGGAATTG...............................................................................................................................................................................................................................	22	0	13	0.38	5	0	1	0	0	0	0	0	0	1	1	1	1	0	0	0	0	0
................................................................................................................................................................................................................GAATACACCGCAACGCCT.........................	18	2	3	0.33	1	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................CGGTAACGCTTCTTGGGGT.................	19	3	4	0.25	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
...................................................................................................................................AGAACACAGCTGGGGGTATCT...................................................................................................	21	3	4	0.25	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
...................................................................................................................................CGTAAACAACTGGGGGTATC....................................................................................................	20	3	5	0.20	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................GGAATGAAACGGAACAGTATC............................................................................................................................................................................	21	3	6	0.17	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................TGAACAGCTGGTGGTATCT...................................................................................................	19	3	20	0.10	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1
...................................................................................................................................TGTAAACAGCTGGAGGTATC....................................................................................................	20	3	10	0.10	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0
....ACTACCGGATGCAGGTCGGCG..................................................................................................................................................................................................................................	21	2	13	0.08	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0
...............TGGGTCGGAGTTGAGGAAG.........................................................................................................................................................................................................................	19	3	15	0.07	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0
................ATGTCGGAATTGAGGATGA........................................................................................................................................................................................................................	19	3	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
........................................................................................................................................................TCTCTTGGTTCTGGCATCG................................................................................	19	3	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0