dvi_8720

CDS [Dvir\GJ16574-cds]; CDS [Dvir\GJ16574-cds]; exon [dvir_GLEANR_17082:5]; exon [dvir_GLEANR_17082:4]; intron [Dvir\GJ16574-in]

Sense Strand Reads

Anti-sense strand reads

##################################################---------------------------------------------------------------------------------------------------------------------------------------------------------################################################## GGCGGCGACTATGACATGCATATGCTGGAACAGCTAAATCACCAGCTAACGTAAGCAACTTAAACCGGGCAGAGCACTCAGTATGCACTTGGCTCAAATCCTCGATTCAATCCATGTTGTTTGCTGCAAGTGCCTGAATCGTGTCGTATATTATTGGAATGTGAAATACCCTGCTATTTCTAATATCGCCTCTCTTCGTGCAGCAATTTTTATTCTACACTGCGTGGCGCTGACTGGCAGCTGCCGAGCTACT ************************************************....(((......(((((.(((.((((((((((.((((.(((....((((...))))....))).)).)).)))))..))))))))..))).))((.((((((..((((((((........))).)))))))))))))..........)))..************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR060673 9_ovaries_total	SRR060683 160_testes_total	SRR060686 Argx9_0-2h_embryos_total	SRR060659 Argentina_testes_total	SRR060668 160x9_males_carcasses_total	M047 female body	SRR1106729 mixed whole adult body	M061 embryo	V053 head	SRR060662 9x160_0-2h_embryos_total	SRR1106725 embryo_14-16h	V116 male body
............GACATGCATATGCTGGAACAGC...........................................................................................................................................................................................................................	22	0	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0
.................GCATATGCTGGAACAGCTGAA.......................................................................................................................................................................................................................	21	1	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0
.............ACATGCATATGCTGGAACAG............................................................................................................................................................................................................................	20	0	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................................GCGTGGCGCAGCCTGGTAGC............	20	3	13	0.38	5	0	0	0	0	0	3	0	1	0	1	0	0
..............CAAGCATGTGCTGGAACAGC...........................................................................................................................................................................................................................	20	2	3	0.33	1	0	0	0	0	1	0	0	0	0	0	0	0
......................TACTGGAACAGCGAAATCA....................................................................................................................................................................................................................	19	2	3	0.33	1	0	0	0	1	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................CGTGGCGCAGCCTGGTAGC............	19	3	20	0.15	3	0	0	0	0	0	0	0	0	2	0	0	1
..........................................................CTTAAACCGGGCAGC....................................................................................................................................................................................	15	1	7	0.14	1	0	0	0	0	0	0	1	0	0	0	0	0
.....................................................................................................TCGATTCAATCGATGTG.......................................................................................................................................	17	2	20	0.05	1	0	0	0	0	0	0	0	0	0	0	1	0
...............AAGCATGTGCTGGAACAGCTG.........................................................................................................................................................................................................................	21	3	20	0.05	1	0	0	0	0	0	0	0	1	0	0	0	0

CCGCCGCTGATACTGTACGTATACGACCTTGTCGATTTAGTGGTCGATTGCATTCGTTGAATTTGGCCCGTCTCGTGAGTCATACGTGAACCGAGTTTAGGAGCTAAGTTAGGTACAACAAACGACGTTCACGGACTTAGCACAGCATATAATAACCTTACACTTTATGGGACGATAAAGATTATAGCGGAGAGAAGCACGTCGTTAAAAATAAGATGTGACGCACCGCGACTGACCGTCGACGGCTCGATGA ************************************************....(((......(((((.(((.((((((((((.((((.(((....((((...))))....))).)).)).)))))..))))))))..))).))((.((((((..((((((((........))).)))))))))))))..........)))..************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR060667 160_females_carcasses_total	SRR060672 9x160_females_carcasses_total	V053 head	SRR060675 140x9_ovaries_total	SRR060657 140_testes_total	SRR060662 9x160_0-2h_embryos_total	SRR060681 Argx9_testes_total	V116 male body
.........................ACCGTGTCGAACTAGTGGTCGAT.............................................................................................................................................................................................................	23	3	1	1.00	1	1	0	0	0	0	0	0	0
..................................................................................TAAGTGAACCGAGTTTAGAAGG.....................................................................................................................................................	22	3	2	0.50	1	0	1	0	0	0	0	0	0
.............................................................................................AGGTTAGGAGGAAAGTTAGGT...........................................................................................................................................	21	3	3	0.33	1	0	0	1	0	0	0	0	0
........................................................................................................................................................................................TAGCGGGGAGATGCACGT...................................................	18	2	3	0.33	1	0	0	0	1	0	0	0	0
...........................................TCGATTGAAGTCGTTGAATTAG............................................................................................................................................................................................	22	3	9	0.11	1	0	0	0	0	1	0	0	0
...........................TTTGTCGATTTATTGGTCGCT.............................................................................................................................................................................................................	21	3	14	0.07	1	0	0	1	0	0	0	0	0
..............................................................................................................................................................................................AGAGAAGCACGTCCATGA.............................................	18	3	20	0.05	1	0	0	0	0	0	1	0	0
.......................................................................................GAAGCGAGTTGACGAGCTA...................................................................................................................................................	19	3	20	0.05	1	0	0	0	0	0	0	0	1
...........................................................AATTTGGCCTGTCTTGTTA...............................................................................................................................................................................	19	3	20	0.05	1	0	0	0	0	0	0	1	0

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droVir3	scaffold_12928:3298862-3299114 -	dvi_8720	GGCGGC---GACTATGACATGCATATGCTGGAACAGCTAAATCACCAGCTAACG--------TAAGCAACTTAAACCGGGCAGAGCACTCAGTATGCACTTGGCTCAAATCCTCGATTCAATCCATGTTGTTTGCTGCAAGTGCCTGAATCGTGTCGTATATTATTGGAATGTGAAATACCCTGCTATTTCTAAT--------------ATCGCCTCT----CTTCGTGCAGCAATTTTTATTCTAC------ACTGCGTGGCGCTGACTGGCAGCTGCCGAGCTACT
droMoj3	scaffold_6308:169669-169849 -		GAGGGC---CCCTACGATATTAATTTGCTGGGTCACCTAAGTTTTCAGCTAACGTAATCAATCGAGT------------------------------CCTTGGCTCACATTCACT----------------------------------------------------------------CTCTGCTACTGCTCTTAACTAATTTGCATTTCCCTCTTT----CTATGTGCAGCAAGTTTTATAATCC------ACTTCGTCGTGCTACTTGGCAACTGCCCAAATATT
droWil2	scf2_1100000004963:4706786-4706801 -		CAA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTGCCCAACTATT
dp5	XL_group1a:3918895-3918946 +		GAGGAA---AATTATGCTCGCCGT---CTGGAAAATCTGCATTTGCAGATAAGG--------TAAG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droPer2	scaffold_11:481859-481910 +		GAGGAA---AATTATGCTCGCGAT---CTGGAAAATCTGCATTTGCAGATAAGG--------TAAG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droAna3	scaffold_13117:2653265-2653268 -		TATT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droFic1	scf7180000454045:326055-326224 -		GGCACA---ATGTACGATATTTGTTTTCTGGAAGACATGAACCTGCGTATAAAG--------TAAGCA------------------------------CATGGAACAAATA-----------------------------------------------------------------------------TGCTTTAGATCAATCAACATGTCTCTTTTTTGTAACATCCACAGTCAATTTTATAATAATAACACTGAGGATGTCAGCGGCTACAGGATGGCGGAGTACT
droEle1	scf7180000491272:221690-221727 +		AACGGC---TTCCAAGACTCCAATATGCTGGCCGAGTTGAA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droRho1	scf7180000779588:250646-250683 -		AATAGC---TCGCACGACACCCAATTGCTGGCCGAGTTGAA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droBia1	scf7180000301345:295839-295847 +		CGAG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GTACT
droTak1	scf7180000415769:426949-426957 -		CGAG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTACT
droYak3	X:3255425-3255478 +		CAGCATCCCACGGACGACTTCGATCCGCTGGTCCAGCTGACCAACGA----AAT--------TAAG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEre2	scaffold_4690:2565814-2565860 -		GATGGC---GA---------CGATCCGCTGGCCGAGCTGAGCAACGAAATGAAG--------TGAGC-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droVir3

GGCGGC---GACTATGACATGCATATGCTGGAACAGCTAAATCACCAGCTAACG--------TAAGCAACTTAAACCGGGCAGAGCACTCAGTATGCACTTGGCTCAAATCCTCGATTCAATCCATGTTGTTTGCTGCAAGTGCCTGAATCGTGTCGTATATTATTGGAATGTGAAATACCCTGCTATTTCTAAT--------------ATCGCCTCT----CTTCGTGCAGCAATTTTTATTCTAC------ACTGCGTGGCGCTGACTGGCAGCTGCCGAGCTACT	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
...............GACATGCATATGCTGGAACAGC...........................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
................ACATGCATATGCTGGAACAG............................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................CTTAAACCGGGCAGC............................................................................................................................................................................................................	15	7	0.14	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0

droMoj3

GAGGGC---CCCTACGATATTAATTTGCTGGGTCACCTAAGTTTTCAGCTAACGTAATCAATCGAGT------------------------------CCTTGGCTCACATTCACT----------------------------------------------------------------CTCTGCTACTGCTCTTAACTAATTTGCATTTCCCTCTTT----CTATGTGCAGCAAGTTTTATAATCC------ACTTCGTCGTGCTACTTGGCAACTGCCCAAATATT	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
No Reads

droWil2

CAA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTGCCCAACTATT	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
No Reads

dp5

GAGGAA---AATTATGCTCGCCGT---CTGGAAAATCTGCATTTGCAGATAAGG--------TAAG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Size

Hit Count

Total Norm

Total

M022
Male-body

M040
Female-body

M059
Embryo

M062
Head

V043
Embryo

V051
Head

V112
Male-body

SRR902010
Ovary

SRR902011
Testis

SRR902012
CNS imaginal disc

No Reads

droPer2

GAGGAA---AATTATGCTCGCGAT---CTGGAAAATCTGCATTTGCAGATAAGG--------TAAG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
No Reads

droAna3

TATT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
No Reads

droFic1

GGCACA---ATGTACGATATTTGTTTTCTGGAAGACATGAACCTGCGTATAAAG--------TAAGCA------------------------------CATGGAACAAATA-----------------------------------------------------------------------------TGCTTTAGATCAATCAACATGTCTCTTTTTTGTAACATCCACAGTCAATTTTATAATAATAACACTGAGGATGTCAGCGGCTACAGGATGGCGGAGTACT	Size	Hit Count	Total Norm	Total
No Reads

droEle1

AACGGC---TTCCAAGACTCCAATATGCTGGCCGAGTTGAA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droRho1

AATAGC---TCGCACGACACCCAATTGCTGGCCGAGTTGAA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droBia1

CGAG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GTACT	Size	Hit Count	Total Norm	Total
No Reads

droTak1

CGAG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTACT	Size	Hit Count	Total Norm	Total
No Reads

droYak3

CAGCATCCCACGGACGACTTCGATCCGCTGGTCCAGCTGACCAACGA----AAT--------TAAG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Size

Hit Count

Total Norm

Total

M026
Head

M043
Female-body

M056
Embryo

V046
Embryo

V052
Head

V058
Head

V120
Male-body

SRR1275488
Male larvae

SRR1275486
Male prepupae

SRR1275484
Male prepupae

GSM1528802
follicle cells

No Reads

droEre2

GATGGC---GA---------CGATCCGCTGGCCGAGCTGAGCAACGAAATGAAG--------TGAGC-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
No Reads

dvi_8720

scaffold_12928:3298912-3299064 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads