| ACCA-------------------------------------ATGGTCACATGTAATTCATTGCATTTTCC--------CCATTTCTTAACG--------------------------------------------TAAAAACCAATCGAA-------------------------------GTCCCATTATACATACAGACGTTC-ATA-------------------------------------------------------------------------------------------------------TATG-----T-ATGT----TTGTACATTCAT----TTGT------------------------------------------------GCAT---------GGGCATCTGTGTGTGTGCGGTTTGTATTTGAGTGCT--TTTTGTTGTTTCTGTTGTG--------TTATTTACCTCCA-TTCTAATT--------------------------------GT-TTTG | Size | Hit Count | Total Norm | Total | M023 Head | M024 Male-body | M025 Embryo | M053 Female-body | O001 Testis | O002 Head | SRR553485 Ovary | SRR553486 Ovary | SRR553487 Ovary | SRR553488 Ovary | SRR618934 Ovary | V044 Embryo | SRR902008 Ovary | SRR902009 Testis | SRR1275487 Male larvae | SRR1275485 Male prepupae | SRR1275483 Male prepupae |
| ............................................................................................................................................................................................................................................................................................................................................................TGT------------------------------------------------GCAT---------GGGCATCTGTGTGTGT.............................................................................................................. | 23 | 1 | 4.00 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................TGT------------------------------------------------GCAT---------GGGCATCTGTGTGTG............................................................................................................... | 22 | 1 | 3.00 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
| .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................GTTTCTGTTGTG--------TTATT........................................................ | 17 | 1 | 2.00 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................TGT------------------------------------------------GCAT---------GGGCATCTGTGTGTGTGC............................................................................................................ | 25 | 1 | 2.00 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................TGTACATTCAT----TTGT------------------------------------------------GCAT---------GGGCATC....................................................................................................................... | 26 | 1 | 2.00 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................TCCCATTATACATACAGACGTTC-ATA-------------------------------------------------------------------------------------------------------.................................................................................................................................................................................................................................. | 26 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................................................................................................................................................................................................................................................................TGTGTGCGGTTTGTATTTGAGTGCT--C....................................................................................... | 26 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................TGT------------------------------------------------GCAT---------GGGCATCTGTGTG................................................................................................................. | 20 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................................................................................................................................................................................................................................................................TGTGTGCGGTTTGTATTTGAGTGC........................................................................................... | 24 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................TGT------------------------------------------------GCAT---------GGGCATCTGTGTGTGTG............................................................................................................. | 24 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................TTATACATACAGACGTTC-ATA-------------------------------------------------------------------------------------------------------A................................................................................................................................................................................................................................. | 22 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................................T----TTGT------------------------------------------------GCAT---------GGGCATCTGTGTGT................................................................................................................ | 23 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................................T----TTGT------------------------------------------------GCAT---------GGGCATCTGTGTG................................................................................................................. | 22 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................................................................................................TTGT------------------------------------------------GCAT---------GGGCATC....................................................................................................................... | 15 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..................................................................................................................................................................................................................................................................................................................................................................................................................................................TGTATTTGAGTGCT--TTTTGTTGTT.............................................................................. | 24 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................ACCAATCGAA-------------------------------GTCCCATTATAC......................................................................................................................................................................................................................................................................................................................................................... | 22 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTGAGTGCT--TTTTGTTGTT.............................................................................. | 20 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTTGTTGTTTCTGTTG....................................................................... | 17 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..................................................TGTAATTCATTGCATTTTCC--------............................................................................................................................................................................................................................................................................................................................................................................................................................................................................ | 20 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................................................................................................TTGT------------------------------------------------GCAT---------GGGCATCTGTGTGTGTG............................................................................................................. | 25 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTGTTGTTTCTGTTGTG--------TTATTTACC.................................................... | 26 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................................................................................................................................................................................................................................................................................T----TTGT------------------------------------------------GCAT---------GGGCATCTGTGTGTG............................................................................................................... | 24 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .................................................................................................................................................................................................................................................................................................................................................................................................................................................TTGTATTTGAGTGCT--TTTTGTTGTT.............................................................................. | 25 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .................................................................................TTTCTTAACG--------------------------------------------TAAAAACCAATCGAA-------------------------------G.................................................................................................................................................................................................................................................................................................................................................................... | 26 | 1 | 1.00 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .......................................................................................................................................................................................CCCATTATACATACAGACGT............................................................................................................................................................................................................................................................................................................................................... | 20 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................................................ATACATACAGACGTTC-ATA-------------------------------------------------------------------------------------------------------T................................................................................................................................................................................................................................. | 20 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................AACCAATCGAA-------------------------------GTCCCATTATACATA...................................................................................................................................................................................................................................................................................................................................................... | 26 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................................................................................................................................................................................TGCGGTTTGTATTTGAGTGCT--TTTTGT.................................................................................. | 27 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..............................................................................................................................................................................................................................................................................................................................................................T------------------------------------------------GCAT---------GGGCATCTGTGTGTGTGCG........................................................................................................... | 24 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| .................................................................................................................................................................................................................................................................................................................................................................................................................................................TTGTATTTGAGTGCT--TTTTGTTGTTTC............................................................................ | 27 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ........................................................................................................................................................................................................................................................................................................................................................................................................................................................TGAGTGCT--TTTTGTTGTTTCTGTTGTG--------T............................................................ | 28 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................................................................................................................................................................................TGT------------------------------------------------GCAT---------GGGCATCTGTGTA................................................................................................................. | 20 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ......................................................................................TAACG--------------------------------------------TAAAAACCAATCGAA-------------------------------GTCCCA............................................................................................................................................................................................................................................................................................................................................................... | 26 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ........................................................................................................................................................................................................................................................................................................................TATG-----T-ATGT----TTGTACA........................................................................................................................................................................................................ | 16 | 9 | 0.11 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |