dvi_8374

CDS [Dvir\GJ16760-cds]; CDS [Dvir\GJ16760-cds]; exon [dvir_GLEANR_17266:2]; exon [dvir_GLEANR_17266:1]; intron [Dvir\GJ16760-in]

Sense Strand Reads

Anti-sense strand reads

##################################################------------------------------------------------------------------------------------------################################################## CCAACGACCAGGATGCCAACAGTATTGCACGCGAGAATGCAGACTACAATGTAAGTCATCATCATCATGGGATTGGTGTGGCTCGTCACATTTGTGAATTTAGATCAGGAAACCATAAAAATTGTTGTGCCTAATTTCAGGCAGCGATTGAAACGCTGACCAAGCTACAGGGAAACGGAGACAGCATACG ************************************************...(((((.(((((((....)).))))))))))..((((....))))....((((.(((....(((((......)))))))).))))...************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR060667 160_females_carcasses_total	M047 female body	SRR060659 Argentina_testes_total	V116 male body	SRR060663 160_0-2h_embryos_total	SRR060688 160_ovaries_total	V047 embryo	SRR060671 9x160_males_carcasses_total	SRR060681 Argx9_testes_total
.............................................................................................................................................................GACCAAGCTACAGGGAAACGGA...........	22	0	1	1.00	1	0	0	1	0	0	0	0	0	0
.........................................................................................................................................................CGCTGACCAAGCTACAGGGAAA...............	22	0	1	1.00	1	1	0	0	0	0	0	0	0	0
.............................................................................................................................................................GACCAAGCTCCAGGGAAACGGAGA.........	24	1	1	1.00	1	1	0	0	0	0	0	0	0	0
....................AGTATTGCACGCGAGAATGCAGAC..................................................................................................................................................	24	0	1	1.00	1	0	0	0	0	0	1	0	0	0
...............................CGAGAATGCAGACTACAAT............................................................................................................................................	19	0	1	1.00	1	0	0	0	0	1	0	0	0	0
.....................................................................................................................................................................TACAGGGAAACGGAGACAGCATACG	25	0	1	1.00	1	0	1	0	0	0	0	0	0	0
.............................................................................................................................................................GACCAAGCTACAGGGAAACG.............	20	0	1	1.00	1	0	1	0	0	0	0	0	0	0
.CAACGACCAGGATGCCAACAGT.......................................................................................................................................................................	22	0	1	1.00	1	1	0	0	0	0	0	0	0	0
.................................................................................................................................................................AAGCTACAGGGAAACGGAGACAGC.....	24	0	1	1.00	1	0	0	1	0	0	0	0	0	0
...............................................................ATCATGGGTTTGGTGGGGCT...........................................................................................................	20	2	1	1.00	1	0	0	0	1	0	0	0	0	0
...............................................................ATCACGGGATCGATGTGGCT...........................................................................................................	20	3	2	0.50	1	0	0	0	1	0	0	0	0	0
............................................................................................................CAAACCATAAAAAGCGTTGTG.............................................................	21	3	6	0.17	1	0	0	0	0	0	0	1	0	0
..................................................................ATGGGATTGGTTTGGGACGT........................................................................................................	20	3	6	0.17	1	0	1	0	0	0	0	0	0	0
.................................................................CATGGGTTTGGTGGGGCT...........................................................................................................	18	2	10	0.10	1	0	1	0	0	0	0	0	0	0
...........GATGCCAACTGTATTGCT.................................................................................................................................................................	18	2	10	0.10	1	0	0	0	0	0	0	0	1	0
......ACCAGGATTCCCTCAGTA......................................................................................................................................................................	18	3	12	0.08	1	0	0	0	0	0	0	0	0	1

GGTTGCTGGTCCTACGGTTGTCATAACGTGCGCTCTTACGTCTGATGTTACATTCAGTAGTAGTAGTACCCTAACCACACCGAGCAGTGTAAACACTTAAATCTAGTCCTTTGGTATTTTTAACAACACGGATTAAAGTCCGTCGCTAACTTTGCGACTGGTTCGATGTCCCTTTGCCTCTGTCGTATGC ************************************************...(((((.(((((((....)).))))))))))..((((....))))....((((.(((....(((((......)))))))).))))...************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR060678 9x140_testes_total	V116 male body	SRR060654 160x9_ovaries_total	SRR1106727 larvae	M061 embryo	M047 female body	V053 head
..........................................TGATGTGACATTAAGTAGTA................................................................................................................................	20	2	1	1.00	1	0	1	0	0	0	0	0
..................................................................................................................................................................TCGGTGTCCCTTTGCCTCTGT.......	21	1	1	1.00	1	1	0	0	0	0	0	0
..............................................................................................................................................TCGCTAACTATGTGAGTGGTT...........................	21	3	2	0.50	1	0	0	1	0	0	0	0
..........CCTAGGGTTGTCATA.....................................................................................................................................................................	15	1	3	0.33	1	0	0	0	1	0	0	0
..............................CGTTCTTGCGTCTGATATTAC...........................................................................................................................................	21	3	6	0.17	1	0	0	0	0	1	0	0
.....................................................................................AGTGTAACCACTTAAAACGA.....................................................................................	20	3	13	0.15	2	0	0	0	0	0	2	0
...................................................................................................................................................AACTTGGCGGCTGGTTC..........................	17	2	16	0.06	1	0	0	0	0	0	0	1

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droVir3	scaffold_12928:1084204-1084393 +	dvi_8374	CCAACGACCAGGATGCCAACAGTATTGCACGCGAGAATGCAGACTACAATGTAAGTCATCATCATCATGGGATTGGTGTGGCTCGTCACATTTGTGAATTTAGATCAGGAAACCATAAAAATTGTTGTGCCT--------------------------AATTTCAGGCAGCGATTGAAACGCTGACCAAGCTACAGGGAAACGGAGACAGCATACG
droMoj3	scaffold_6328:2062866-2063012 +		TCACCGAGCAGGATGCC------------CGGGAGGAAGCCGAATACAATGTAAGTTTACTGCCTAACTGGGGTGCAGAGATCCCTTATTT-------------------------------GTTTCTTGTT--------------------------GCTTCTAGGCTGACATTGAGCAGCTGAAGATTCTGCAGTCGAATCGGGAAAGCATCCA
droGri2	scaffold_14853:7471585-7471752 -		AA-----------TGAC------------CAAGATAATGCCGCTTACATGGTAAGCACTTATTT-----CAAATATTCTGATTTGAAATATTAGTATTT--CATT-TG-----------------CATTGTTTCTGTGATATATTAATAATGTTGTGAATTTGTAGGCTGCAATTGAGGTGCTGACAACACTGCAGCCAAACAAGGACAGCATAAG
droWil2	scf2_1100000004909:1857341-1857403 +		TG-------------------------------------------------------------------------------------------------------------------------------CTC--------------------------CATCATAGGAAGCCATTCAAGCACTGAACTCTCTGCAAAGCAATGACAATGCCATACG
dp5	XL_group1a:3285928-3285986 +		T-------------------------------------------------------------------------------------------------------------------------------------------------------------ATTTTCAGGCGGCCATCAAGCAGCTAAACATTCTGCAGTCGAACGATGTTACAATACG
droPer2	scaffold_77:223404-223462 -		T-------------------------------------------------------------------------------------------------------------------------------------------------------------ATTTTCAGGCGGCCATCAAGCAGCTAAACATTCTGCAGTCGAACGATGTTACAATACG
droAna3	scaffold_13117:3400272-3400324 +		C-------------------------------------------------------------------------------------------------------------------------------------------------------------------AGAATGCTATTAAGAAATTAAACTCCCTGCAGTCGAACGATGCTGCCATCCG
droBip1	scf7180000396015:4091-4146 -		T----------------------------------------------------------------------------------------------------------------------------------------------------------------TTTAGATTGCTATTCAAAAACTAAACTCTCTGCAGTCCAACGATGCTGCCCTCCG
droKik1	scf7180000302696:363741-363796 -		T----------------------------------------------------------------------------------------------------------------------------------------------------------------TGCAGGCAGCCATCGCTCAGCTTAACTTCCTGCAATCCAACGAGGACACCATCAG
droFic1	scf7180000453882:257549-257604 +		T----------------------------------------------------------------------------------------------------------------------------------------------------------------TTTAGGAGGCCATCAAAAAGCTAAATTCTCTGCAGTCGAACGATGCTGCCATTCG
droRho1	scf7180000779985:182544-182609 -		T----------------------------------------------------------------------------------------------------------------------------TCTAACT--------------------------ATTTCCAGGAGGCCATAAAGCGGCTAAACTCCCTGCAGTCAAACGATGCTGCTATAAG
droBia1	scf7180000298389:66314-66394 +		ATAAAAA------TGCA------------CTA-------------------------------------------------------------------------------------------TTTTTTGCC--------------------------GTTTTCAGGAGGCCATCGTGCAGTTGAACTGCCTGCAGTCCAACGACGCTGCCATAAG
droTak1	scf7180000415866:121543-121640 -		TA--------------------------------------------------------------------------------------------TTAAGTTAAAAACGATAATAAAAGAGACTTTTTTGGTC--------------------------ATTTTCAGGAGGCCATCAAGCAGCTGAACTCCCTGCAGTCGAACGATGCCGCCATAAG
droEug1	scf7180000409110:892783-892838 +		T----------------------------------------------------------------------------------------------------------------------------------------------------------------TTCAGGAGGCTATCAGGAAGTTAAACTCTTTGCAGTCGAACGATGCTGCTATTAA
droSim2	x:11863401-11863460 +		CT------------------------------------------------------------------------------------------------------------------------------------------------------------ACTTTCAGTTGGCCATCAAGCAGTTGAATTCTCTGCAGTCGAACGATGCTGCCATAAG
droSec2	scaffold_21:261584-261643 +		CC------------------------------------------------------------------------------------------------------------------------------------------------------------ACTTTCAGTTGGCCATCAAGCAGTTGAATTCTCTGCAGTCGAACGATGCTGCCATAAG

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droVir3

CCAACGACCAGGATGCCAACAGTATTGCACGCGAGAATGCAGACTACAATGTAAGTCATCATCATCATGGGATTGGTGTGGCTCGTCACATTTGTGAATTTAGATCAGGAAACCATAAAAATTGTTGTGCCT--------------------------AATTTCAGGCAGCGATTGAAACGCTGACCAAGCTACAGGGAAACGGAGACAGCATACG	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
.......................................................................................................................................................................................GACCAAGCTACAGGGAAACGGA...........	22	1	1.00	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................CGCTGACCAAGCTACAGGGAAA...............	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................AGTATTGCACGCGAGAATGCAGAC............................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................CGAGAATGCAGACTACAAT......................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................TACAGGGAAACGGAGACAGCATACG	25	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................GACCAAGCTACAGGGAAACG.............	20	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.CAACGACCAGGATGCCAACAGT.................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................................................................................................................................................................................AAGCTACAGGGAAACGGAGACAGC.....	24	1	1.00	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droMoj3

TCACCGAGCAGGATGCC------------CGGGAGGAAGCCGAATACAATGTAAGTTTACTGCCTAACTGGGGTGCAGAGATCCCTTATTT-------------------------------GTTTCTTGTT--------------------------GCTTCTAGGCTGACATTGAGCAGCTGAAGATTCTGCAGTCGAATCGGGAAAGCATCCA	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
...........GATGCC------------CGGGAGGAAGCCGA.............................................................................................................................................................................	20	1	3.00	3	1	0	0	0	0	2
............ATGCC------------CGGGAGGAAGCCGA.............................................................................................................................................................................	19	1	1.00	1	1	0	0	0	0	0
........CAGGATGCC------------CGGGAGGAAGCCGA.............................................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	1
............ATGCC------------CGGGAGGAAGCCG..............................................................................................................................................................................	18	1	1.00	1	1	0	0	0	0	0
.........AGGATGCC------------CGGGAGGAAGC................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	1

droGri2

AA-----------TGAC------------CAAGATAATGCCGCTTACATGGTAAGCACTTATTT-----CAAATATTCTGATTTGAAATATTAGTATTT--CATT-TG-----------------CATTGTTTCTGTGATATATTAATAATGTTGTGAATTTGTAGGCTGCAATTGAGGTGCTGACAACACTGCAGCCAAACAAGGACAGCATAAG	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

droWil2

TG-------------------------------------------------------------------------------------------------------------------------------CTC--------------------------CATCATAGGAAGCCATTCAAGCACTGAACTCTCTGCAAAGCAATGACAATGCCATACG	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
No Reads

dp5

T-------------------------------------------------------------------------------------------------------------------------------------------------------------ATTTTCAGGCGGCCATCAAGCAGCTAAACATTCTGCAGTCGAACGATGTTACAATACG	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
.....................................................................................................................................................................................CTAAACATTCTGCAGTCGAACGATGTT........	27	1	1.00	1	0	0	0	1	0	0	0	0	0	0

droPer2

T-------------------------------------------------------------------------------------------------------------------------------------------------------------ATTTTCAGGCGGCCATCAAGCAGCTAAACATTCTGCAGTCGAACGATGTTACAATACG	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
...........................................................................................................................................................................................ATTCTGCAGTCGAACGATGTTACA.....	24	1	1.00	1	0	0	1	0	0	0

droAna3

C-------------------------------------------------------------------------------------------------------------------------------------------------------------------AGAATGCTATTAAGAAATTAAACTCCCTGCAGTCGAACGATGCTGCCATCCG	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
...................................................................................................................................................................................................GTCGAACGATGCTGCCATCC.	20	1	1.00	1	0	1	0	0	0	0
............................................................................................................................................................................................CCCTGCAGTCGAACGATGC.........	19	1	1.00	1	0	1	0	0	0	0

droBip1

T----------------------------------------------------------------------------------------------------------------------------------------------------------------TTTAGATTGCTATTCAAAAACTAAACTCTCTGCAGTCCAACGATGCTGCCCTCCG	Size	Hit Count	Total Norm	Total
No Reads

droKik1

T----------------------------------------------------------------------------------------------------------------------------------------------------------------TGCAGGCAGCCATCGCTCAGCTTAACTTCCTGCAATCCAACGAGGACACCATCAG	Size	Hit Count	Total Norm	Total
No Reads

droFic1

T----------------------------------------------------------------------------------------------------------------------------------------------------------------TTTAGGAGGCCATCAAAAAGCTAAATTCTCTGCAGTCGAACGATGCTGCCATTCG	Size	Hit Count	Total Norm	Total
No Reads

droRho1

T----------------------------------------------------------------------------------------------------------------------------TCTAACT--------------------------ATTTCCAGGAGGCCATAAAGCGGCTAAACTCCCTGCAGTCAAACGATGCTGCTATAAG	Size	Hit Count	Total Norm	Total
No Reads

droBia1

ATAAAAA------TGCA------------CTA-------------------------------------------------------------------------------------------TTTTTTGCC--------------------------GTTTTCAGGAGGCCATCGTGCAGTTGAACTGCCTGCAGTCCAACGACGCTGCCATAAG	Size	Hit Count	Total Norm	Total
No Reads

droTak1

TA--------------------------------------------------------------------------------------------TTAAGTTAAAAACGATAATAAAAGAGACTTTTTTGGTC--------------------------ATTTTCAGGAGGCCATCAAGCAGCTGAACTCCCTGCAGTCGAACGATGCCGCCATAAG	Size	Hit Count	Total Norm	Total
No Reads

droEug1

T----------------------------------------------------------------------------------------------------------------------------------------------------------------TTCAGGAGGCTATCAGGAAGTTAAACTCTTTGCAGTCGAACGATGCTGCTATTAA	Size	Hit Count	Total Norm	Total
No Reads

droSim2

CT------------------------------------------------------------------------------------------------------------------------------------------------------------ACTTTCAGTTGGCCATCAAGCAGTTGAATTCTCTGCAGTCGAACGATGCTGCCATAAG	Size	Hit Count	Total Norm	Total	M023 Head	M024 Male-body	M025 Embryo	M053 Female-body	O001 Testis	O002 Head	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary	V044 Embryo	SRR902008 Ovary	SRR902009 Testis	SRR1275487 Male larvae	SRR1275485 Male prepupae	SRR1275483 Male prepupae
....................................................................................................................................................................................GTTGAATTCTCTGCAGTCGAACGA............	24	1	1.00	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0

droSec2

CC------------------------------------------------------------------------------------------------------------------------------------------------------------ACTTTCAGTTGGCCATCAAGCAGTTGAATTCTCTGCAGTCGAACGATGCTGCCATAAG	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
No Reads

dvi_8374

scaffold_12928:1084254-1084343 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads