dvi_5983

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------################################################## TGCCATATTGTCAGTTTAATTTGTCTTAAATATTAAAGTTCTTAACTTTATAAATAAATGAGCATAAATATATTTTTGTTCGAGCTGCTGTTGCACTTGAAATGGAAAATGTCTGCCATTTTGTAGCGCCACAGATTCCCAGCCACACATTTATGTGTATGCGAAGAAGCCCATATATACCATATATTTTGATACATTTAGAAAATGGCTTCTCGCATCCCTCGCAAAATCGTGATGCTCAGATTTGCGAC ************************************************....((((((.....((((((((..........((((((((.((.((((((((((..........)))))))).)).)).)))).....))))........(((((((..((......))..)))))))..)))))))).....)))))).************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M028 head	SRR1106729 mixed whole adult body	M061 embryo	V053 head	SRR1106727 larvae	V047 embryo	SRR060684 140x9_0-2h_embryos_total	SRR060663 160_0-2h_embryos_total	SRR1106728 larvae	M047 female body	SRR060669 160x9_females_carcasses_total
..............................................................................................................................................................................................................................CGCAAAGTCGTGAGGCT............	17	2	15	1.67	25	0	17	0	0	6	0	0	0	2	0	0
..........................................................................TTTGTTCGAGCTGCTGTT...............................................................................................................................................................	18	0	2	1.50	3	3	0	0	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................TGGCTTCTCGCTTCCCTCCCC.........................	21	3	2	0.50	1	0	0	0	1	0	0	0	0	0	0	0
.....................................................................................................................................................TTTATGTGTACGCCAAGAATC.................................................................................	21	3	2	0.50	1	0	0	1	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................GCGAAGTCGTGATGCTAAG.........	19	3	6	0.17	1	0	0	0	0	0	0	0	1	0	0	0
............................................................................................................................................................GTATGCGACGAAGCCTAGA............................................................................	19	3	7	0.14	1	0	0	0	0	0	1	0	0	0	0	0
.........................................................................................GTTGCACTTGAAAGGGAAG...............................................................................................................................................	19	2	9	0.11	1	0	0	0	0	0	0	1	0	0	0	0
............................................................................................................................................................................................TTGATACAACTACAAAATGGC..........................................	21	3	9	0.11	1	0	0	0	0	0	0	0	0	0	1	0
.............................................................................................................................................................................................................................TCGCAAAGTCGTGAGGCT............	18	2	10	0.10	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................CTCGCGAAGTCGTGACGCT............	19	3	11	0.09	1	0	0	0	0	0	1	0	0	0	0	0
..........................................................................................CTGCACTTGCAATGGAAAA..............................................................................................................................................	19	2	12	0.08	1	0	0	0	0	0	0	1	0	0	0	0
.............................................................................................CACATGAACTGGAAAATG............................................................................................................................................	18	2	19	0.05	1	0	0	0	0	0	0	0	0	0	0	1

ACGGTATAACAGTCAAATTAAACAGAATTTATAATTTCAAGAATTGAAATATTTATTTACTCGTATTTATATAAAAACAAGCTCGACGACAACGTGAACTTTACCTTTTACAGACGGTAAAACATCGCGGTGTCTAAGGGTCGGTGTGTAAATACACATACGCTTCTTCGGGTATATATGGTATATAAAACTATGTAAATCTTTTACCGAAGAGCGTAGGGAGCGTTTTAGCACTACGAGTCTAAACGCTG ************************************************....((((((.....((((((((..........((((((((.((.((((((((((..........)))))))).)).)).)))).....))))........(((((((..((......))..)))))))..)))))))).....)))))).************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR060669 160x9_females_carcasses_total	V053 head	SRR060682 9x140_0-2h_embryos_total
....TATAACACTCAAATTAAA.....................................................................................................................................................................................................................................	18	1	2	0.50	1	1	0	0
...........................................................................................................................................................GCATACGCTTCCTCGGGTGT............................................................................	20	3	5	0.20	1	0	1	0
.......................................................................................................................AAACATTGCGATGTCTAAAG................................................................................................................	20	3	13	0.08	1	0	0	1

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droVir3	scaffold_12875:10706663-10706913 +	dvi_5983	TGCCATATTGTCAGTTTAATTTGTCTTAAATATTAAAGTTCTTAACTTTATAAATAAATGAGCA----------TAAATA-----TATT-TTTGTTCGAGCTGCTGTTGCACTTGAAATGGAAAATGTCTGCCATTTTGTAGCGCCACAGATTCCCAGCCACACATTTATGTGTATGCGAAGAAGCCCATATATACCA-TATATTTTGATACATTTAGAAAATGGCTTCTCGCATCCCTCGCAAAATCGTGATGCTCAGATTTGCGAC
droMoj3	scaffold_4164:3203-3297 +		TCAGTCAA----------ATTTGTAAAAAACAACAAAGTTCAAAGCTGTATAAAAAAAAAATAA----------AACATTTTATCTTATTTCATCTCGAAATGCCATT-----TGAAATG----------------------------------------------------------------------------------------------------------------------------------------------------
droGri2	scaffold_15245:3815256-3815366 +		GCAAC-----------------A-------------------------------TCTCTATATATATATCTATATATATA-----TATC-T-------------------------------------------------------------------------------------AT--------ACATATATACCAATGTAT--------TATTACAAAATGGC--CTCGCATCCCTTGCAAAAACCGAATGCTTAGATTTGCGTC
droWil2	scf2_1100000004822:3487650-3487772 +		TATAA-----------------A-------------------------------TATATATATA----------TATATT-----TTTT-ATTGTCAGAGTCGCAGTCGCAGTTCGCATTAAAAATACTTAATCTCCAATGGCACTAAAA--------ACACACATACACATACATACATAGAGACACGGACACA-------------------------------------------------------------------------
dp5	XL_group1a:4181204-4181259 +		TATTTTATTTTTATATTTTTTTGTATAAAATATTATTTTTTTTAACTTTATATGTA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droPer2	scaffold_22:1067569-1067627 +		CCCCTAATT------------------------------------------------------------------------------------------------------------------------------------------------TCCAACCATCCATATAGATGTATGTATGTAAATCCATATACATGC-TATAT----------------------------------------------------------------
droKik1	scf7180000302621:296238-296275 +		AATTT-----------------GTCTTTAATATTAAAATTCATTAAATTATTAAT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEle1	scf7180000491214:3813527-3813570 -		AAATA---------------------------------------------------AATGACAA----------TAAATA-----TTAT-TTGGCTAGAGTTGTTGCTTCA-------------------------------------------------------------------------------------------------------------------------------------------------------------
droRho1	scf7180000770948:55752-55817 -		TATTATACA-----TTTAATTTGACTTTTATATTAGCAGTTTTAATTTTAAAAA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AAGTGTACATATGTTTT
droTak1	scf7180000414560:184671-184720 +		CATTTT-----------TACTTTTCCAAAATATTAAAATTTTTAAAATCGTATATAAGCGA---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEre2	scaffold_4845:8428264-8428300 -		GCATG-----------------------------------------------------------------------TATA-----TGTT-GTTGTTTCGGTTGCTGTTGCATTT----------------------------------------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droVir3

TGCCATATTGTCAGTTTAATTTGTCTTAAATATTAAAGTTCTTAACTTTATAAATAAATGAGCA----------TAAATA-----TATT-TTTGTTCGAGCTGCTGTTGCACTTGAAATGGAAAATGTCTGCCATTTTGTAGCGCCACAGATTCCCAGCCACACATTTATGTGTATGCGAAGAAGCCCATATATACCA-TATATTTTGATACATTTAGAAAATGGCTTCTCGCATCCCTCGCAAAATCGTGATGCTCAGATTTGCGAC	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
..........................................................................................TTTGTTCGAGCTGCTGTT................................................................................................................................................................	18	2	1.50	3	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droMoj3

TCAGTCAA----------ATTTGTAAAAAACAACAAAGTTCAAAGCTGTATAAAAAAAAAATAA----------AACATTTTATCTTATTTCATCTCGAAATGCCATT-----TGAAATG----------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
No Reads

droGri2

GCAAC-----------------A-------------------------------TCTCTATATATATATCTATATATATA-----TATC-T-------------------------------------------------------------------------------------AT--------ACATATATACCAATGTAT--------TATTACAAAATGGC--CTCGCATCCCTTGCAAAAACCGAATGCTTAGATTTGCGTC	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

droWil2

TATAA-----------------A-------------------------------TATATATATA----------TATATT-----TTTT-ATTGTCAGAGTCGCAGTCGCAGTTCGCATTAAAAATACTTAATCTCCAATGGCACTAAAA--------ACACACATACACATACATACATAGAGACACGGACACA-------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
No Reads

dp5

TATTTTATTTTTATATTTTTTTGTATAAAATATTATTTTTTTTAACTTTATATGTA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Size

Hit Count

Total Norm

Total

M022
Male-body

M040
Female-body

M059
Embryo

M062
Head

V043
Embryo

V051
Head

V112
Male-body

SRR902010
Ovary

SRR902011
Testis

SRR902012
CNS imaginal disc

No Reads

droPer2

CCCCTAATT------------------------------------------------------------------------------------------------------------------------------------------------TCCAACCATCCATATAGATGTATGTATGTAAATCCATATACATGC-TATAT----------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
No Reads

droKik1

AATTT-----------------GTCTTTAATATTAAAATTCATTAAATTATTAAT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEle1

AAATA---------------------------------------------------AATGACAA----------TAAATA-----TTAT-TTGGCTAGAGTTGTTGCTTCA-------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droRho1

TATTATACA-----TTTAATTTGACTTTTATATTAGCAGTTTTAATTTTAAAAA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AAGTGTACATATGTTTT	Size	Hit Count	Total Norm	Total
No Reads

droTak1

CATTTT-----------TACTTTTCCAAAATATTAAAATTTTTAAAATCGTATATAAGCGA---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEre2

GCATG-----------------------------------------------------------------------TATA-----TGTT-GTTGTTTCGGTTGCTGTTGCATTT----------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
No Reads

dvi_5983

scaffold_12875:10706713-10706863 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-28.8	-28.7	-28.6