dvi_5936

##################################################--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- ATCAGGGGTAGCCCTGCTACTACTGGTATTACATGCCAATAATCCAAGTGGTACGTCAAATCAAGAGTGATTAGCAAGGATATGCTAGTTCAAGTGTGGTGCTTAAATGTACAAGATTTATTGTTACAAGGGTGTTGATAAATCAATACATATCGCGAATGATAAATCAAAGCAGAAATATCTAAATGGAATTCGAAAATCACTAAAAAAATTGTTTAAATATCCTTTAGGACATATTCCAAGGGACGATA *******************************************************..((((....(((((((((......)))))).)))....))))((((...(((((((......))).)))).))))********************************************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M047 female body	M061 embryo	V047 embryo	M027 male body	SRR060671 9x160_males_carcasses_total	SRR1106727 larvae	GSM1528803 follicle cells	SRR060681 Argx9_testes_total	SRR060682 9x140_0-2h_embryos_total	SRR060686 Argx9_0-2h_embryos_total	SRR060675 140x9_ovaries_total	SRR060665 9_females_carcasses_total	SRR060672 9x160_females_carcasses_total
.........AGCCCTGCTACTACTGGTATTACATGC.......................................................................................................................................................................................................................	27	0	1	2.00	2	1	0	1	0	0	0	0	0	0	0	0	0	0
.........AGCCCTGCTACTACTGGTATT.............................................................................................................................................................................................................................	21	0	1	2.00	2	2	0	0	0	0	0	0	0	0	0	0	0	0
.........ATCCCTGCTACTACTGGTATTACATGC.......................................................................................................................................................................................................................	27	1	1	2.00	2	0	2	0	0	0	0	0	0	0	0	0	0	0
....................................CAATAATCCAAGTGGTACGTC..................................................................................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0
..........GCCCTGCTACTACTGGTATTACATGC.......................................................................................................................................................................................................................	26	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0
.........AGCCCTGCTACTACTGGTATTA............................................................................................................................................................................................................................	22	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0
...................CTACTGGTATTACATGCCA.....................................................................................................................................................................................................................	19	0	1	1.00	1	0	0	0	0	1	0	0	0	0	0	0	0	0
..............................ACATGCCAATAATCCAAGTGACG......................................................................................................................................................................................................	23	3	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0
...........................ATTACATGCCAATAATCCAA............................................................................................................................................................................................................	20	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0
.........AGCCCTGCTACTACTGGTATTACATGCC......................................................................................................................................................................................................................	28	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................AAATCAAGAGTGATTAGCAAGG............................................................................................................................................................................	22	0	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0
............................TTACATGCCAATAATCCAAGT..........................................................................................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0
........TAGCCCTGCTACTACTGGTATT.............................................................................................................................................................................................................................	22	0	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0	0
.................................................................................................................AGATTTATTGTTACAAGGGT......................................................................................................................	20	0	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0	0
................CTACTACTGGTATTACATGCC......................................................................................................................................................................................................................	21	0	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0	0
........................GGTATTACATGCCAATAATCCAAGT..........................................................................................................................................................................................................	25	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0
........GAGCCCTGCTACTACTGGT................................................................................................................................................................................................................................	19	1	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................................................................................................TATCGCGAATGATAAATCAAAGCAGAAATATCTAAA.................................................................	36	0	1	1.00	1	0	0	0	0	0	1	0	0	0	0	0	0	0
.......................................................................................GTTCAAGTGTGGTGCTTAAATGT.............................................................................................................................................	23	0	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0
.........AGCCCTGCTACTACTGGT................................................................................................................................................................................................................................	18	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................................AATCACTAAAAAAATTGTT...................................	19	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................................AGTTCAAGTCTGGTGC.....................................................................................................................................................	16	1	3	0.67	2	0	0	0	0	0	0	2	0	0	0	0	0	0
.......................................................................................GTTCGAGTGAGGTGCTTAAAG...............................................................................................................................................	21	3	2	0.50	1	0	0	0	0	0	0	0	1	0	0	0	0	0
..........GCCGTGCTACTATTGGTACT.............................................................................................................................................................................................................................	20	3	2	0.50	1	0	0	0	0	0	0	0	0	1	0	0	0	0
.....................................................................................................................................................ATATCGCGAATGACATCTCA..................................................................................	20	3	4	0.25	1	0	0	0	0	0	0	0	0	0	1	0	0	0
........................................................................AGCAAGGACGAGCTAGTTC................................................................................................................................................................	19	3	18	0.11	2	0	0	0	0	0	0	0	0	0	0	0	1	1
..................................TCCAAGAATCCAAGTGGAAC.....................................................................................................................................................................................................	20	3	12	0.08	1	0	0	0	0	0	0	0	0	0	0	1	0	0

TAGTCCCCATCGGGACGATGATGACCATAATGTACGGTTATTAGGTTCACCATGCAGTTTAGTTCTCACTAATCGTTCCTATACGATCAAGTTCACACCACGAATTTACATGTTCTAAATAACAATGTTCCCACAACTATTTAGTTATGTATAGCGCTTACTATTTAGTTTCGTCTTTATAGATTTACCTTAAGCTTTTAGTGATTTTTTTAACAAATTTATAGGAAATCCTGTATAAGGTTCCCTGCTAT ********************************************************************************************************************..((((....(((((((((......)))))).)))....))))((((...(((((((......))).)))).))))*******************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR060676 9xArg_ovaries_total	V116 male body	SRR060673 9_ovaries_total	V053 head	SRR060686 Argx9_0-2h_embryos_total	V047 embryo
...................................................ATGCAGTTTGGTGGTCACT.....................................................................................................................................................................................	19	3	1	1.00	1	0	1	0	0	0	0
.........................................................................CGTTCCTATGCGATCAAGTTCACACCA.......................................................................................................................................................	27	1	1	1.00	1	1	0	0	0	0	0
....................................................................................................................................................................................................................................TTCTGTATAAGGTTTCCTGTTAT	23	3	2	0.50	1	0	0	1	0	0	0
........ATCGGATCGATGAAGACCAT...............................................................................................................................................................................................................................	20	3	4	0.25	1	0	0	0	0	1	0
......................................................CGGTTTAGTTCCCACTAA...................................................................................................................................................................................	18	2	5	0.20	1	0	0	0	0	0	1
.....................TGACCACAATATCCGGTTATT.................................................................................................................................................................................................................	21	3	5	0.20	1	0	0	0	1	0	0
....................ATGACGACACTGTACGGTTATT.................................................................................................................................................................................................................	22	3	6	0.17	1	0	0	0	1	0	0

Species	Coordinate	ID	Alignment
droVir3	scaffold_12875:10277995-10278245 -	dvi_5936	ATCAGGG---GTAGCCCTGCTACTACTGGTATTACATGCCAATAATCCAAGT------GGTACGTCAAATCAAGAGTGATTAGCAAGGATA--------------------------------------------------------------------------TGCTAGTTCAAGTGTGGTGCTTAAATGTACAAGATTTATTGTTACAAGGGTGTTGATAAATCAATACATATCGCGAATGATAAA-TC-AAAGCAGAAATA---TCTAAATGGAATTCGAAAATCACTAAAAAAATTGTTTAAATATCCTTTAGGACATATTCCAAGGGACGATA
droMoj3	scaffold_6496:16722768-16722873 +		AGCAGGGATAATAACTCTGCTACAAATAGTATTATATGTGGCTAATCCAAGT------GGTAGGTCAAGTCAAGTGTGATCAACAAGGATAATT-----------------------------------------------------------------------TGTTAATTCAAGTGTTGT------------------------------------------------------------------------------------------------------------------------------------------------------------
droGri2	scaffold_15245:3425090-3425192 -		AGTAGGA---GTAGCTCTGCTACTTCTGGTATTATATGCCAATAATCCAAGT------GGTAGGTGGACTCAAGTGTGATAATCAAGGATAACG-----------------------------------------------------------------------T----GCTCAAGTGTTGTGCTT--------------------------------------------------------------------------------------------------------------------------------------------------------
droWil2	scf2_1100000004514:3004366-3004421 -		TGTAGGA---GTAGCTTGTCTACTTCTGGTCCTCAGTGCCTTCAATCCAACT------GGTAGGT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
dp5	3:15273297-15273346 -		TGCAGGA---GCAGCCCTGCTGCTTCTTGCACTAAGTGCAAATCAAACCAGT------G----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droPer2	scaffold_4:3778179-3778249 +		TGCAGGC---GCAGCCCTGCTGCTTCTTGCACTAAGTGCAAATCAATCCAGTGCCAATGGTAGGTGGGCCCAAG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droAna3	scaffold_13266:11095498-11095578 -		AGCAGGA---GTAGCCCTTCTACTTCTAGTGCTAAATACAAATTCCATAAGT------GGTAGGTGCTCCCGAGAAAGAGGATCAAGGAT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droBip1	scf7180000396547:1372946-1373067 +		AACAGGA---GTAGCCCTGCTACTTCTAGTGCTTAACACAAATTCCATAAGT------GGTAGGTGTACCCGAGAAAGAGAATCAAGGATATA------TCCA---------------------------------TTC--------------------------TCCCAAATCAAGTGCTGA-----AAAC------------------------------------------------------------------------------TAA--GATATT----------------------------------------------------------
droKik1	scf7180000302366:237492-237696 +		AGCAGGA---GTAGCCTTGCTACTTCTTGTGCTAA------GCCCAGCGAGT------GGTAGGTGTTGCC---------GATCAAGGATAAAGGATAATACCAAGTGAAAGTGAAATATAAACACAAAACGGTCATTCTTAAACTGAAATCTTCAAGGATAATCAGAAAATAAAAGTGAA------------------------------------------------------------AATATAAACTATAAAACAGTAAAAATTGC--------------------TTCAAAAAATTATATAAAT------------------------------
droFic1	scf7180000453858:126873-126922 -		GGCAGGA---GTAGCACTGCTACTTCTCGTGTTAA------ATCATTCAAGT------GGTAGGT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEle1	scf7180000487266:235-347 +		TGCAGGA---GTAGCCCTGCTACTTCTTGTGATAA------ATCAGTCAAGT------GGTAGGTGTT----TGTC--TAAACCAAGGACGAAGGATAATCCAAAGTTAAAGTGGT------------------CG-----------------------------TGATAGTTCAAGTGCG--------------------------------------------------------------------------------------------------------------------------------------------------------------
droBia1	scf7180000301754:4818288-4818359 -		AGCAGGA---GTAGCCCTGCTACTTCTAGTGCTGA------ATCAGTCAAGT------GGTAGGTTTT--CGAGC---CAAGTCAAGGATAA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droTak1	scf7180000415681:290859-291042 -		AGCAGGA---GTAGCCCTGCTACTTCTAGTGCTAA------ACCAGTCAAGT------GGTAGGTTGAA-CAAGC---ACCATCAAGGATAAAGGATAATCCAAAGTGAAAGTGAA------------------AG-----------------------------TG-------------------------------GACTACCACCAAAAAAGTGCCA---------------------AATATGAACAA-AAAACAGAAGAA---GCTAA--GAAATTTTAAAGAAACTAACCAAGTTATATAAA-------------------------------
droEug1	scf7180000409672:7546089-7546138 -		AGCAGGA---GTAGCCTTGCTACTTCTAGTTTTAA------ATCACTCAAGT------GGTAGGT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droYak3	2R:11469039-11469116 +		GGCAGGA---GTAGCTCTGCTACTCCTTGTGCTAA------ATCACTCAAGT------GGTAGGTTC----CAGTC--GAAATCCAGGATTAAGATTAG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEre2	scaffold_4845:11850059-11850127 -		GGCAGGA---GTAGCTCTGCTACTTCTTGTGCTAA------ATCACTCAAGT------GGTAGGTT----CAAGTG--TAAATCAAGGAT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droVir3

ATCAGGG---GTAGCCCTGCTACTACTGGTATTACATGCCAATAATCCAAGT------GGTACGTCAAATCAAGAGTGATTAGCAAGGATA--------------------------------------------------------------------------TGCTAGTTCAAGTGTGGTGCTTAAATGTACAAGATTTATTGTTACAAGGGTGTTGATAAATCAATACATATCGCGAATGATAAA-TC-AAAGCAGAAATA---TCTAAATGGAATTCGAAAATCACTAAAAAAATTGTTTAAATATCCTTTAGGACATATTCCAAGGGACGATA	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
............AGCCCTGCTACTACTGGTATTACATGC............................................................................................................................................................................................................................................................................................................	27	1	2.00	2	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
............AGCCCTGCTACTACTGGTATT..................................................................................................................................................................................................................................................................................................................	21	1	2.00	2	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................CAATAATCCAAGT------GGTACGTC.................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............GCCCTGCTACTACTGGTATTACATGC............................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............AGCCCTGCTACTACTGGTATTA.................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................CTACTGGTATTACATGCCA..........................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................ACATGCCAATAATCCAAGT------GACG.....................................................................................................................................................................................................................................................................................	23	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................ATTACATGCCAATAATCCAA.................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............AGCCCTGCTACTACTGGTATTACATGCC...........................................................................................................................................................................................................................................................................................................	28	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................AAATCAAGAGTGATTAGCAAGG...........................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
...............................TTACATGCCAATAATCCAAGT------.........................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........TAGCCCTGCTACTACTGGTATT..................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................AGATTTATTGTTACAAGGGT...........................................................................................................................	20	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...................CTACTACTGGTATTACATGCC...........................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................GGTATTACATGCCAATAATCCAAGT------.........................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................................................................TATCGCGAATGATAAA-TC-AAAGCAGAAATA---TCTAAA.................................................................	36	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0
..........................................................................................................................................................................GTTCAAGTGTGGTGCTTAAATGT..................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
............AGCCCTGCTACTACTGGT.....................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................................................................................................AATCACTAAAAAAATTGTT...................................	19	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droMoj3

AGCAGGGATAATAACTCTGCTACAAATAGTATTATATGTGGCTAATCCAAGT------GGTAGGTCAAGTCAAGTGTGATCAACAAGGATAATT-----------------------------------------------------------------------TGTTAATTCAAGTGTTGT------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
....................TACAAATAGTATTATATGTGG..........................................................................................................................................................................................................................................................................................................	21	1	2.00	2	2	0	0	0	0	0
............AACTCTGCTACAAATAGTATTATATGT............................................................................................................................................................................................................................................................................................................	27	1	1.00	1	1	0	0	0	0	0
........................AATAGTATTATATGTGGCT........................................................................................................................................................................................................................................................................................................	19	1	1.00	1	1	0	0	0	0	0
............................GTATTATATGTGGCTAATCC...................................................................................................................................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
.....GGATAATAACTCTGCTACAAATAGTATT..................................................................................................................................................................................................................................................................................................................	28	1	1.00	1	1	0	0	0	0	0
......................CAAATAGTATTATATGTGG..........................................................................................................................................................................................................................................................................................................	19	1	1.00	1	1	0	0	0	0	0
..........................................TAATCCAAGT------GGTAGGTCAAGTCAAGT........................................................................................................................................................................................................................................................................	27	1	1.00	1	1	0	0	0	0	0

droGri2

AGTAGGA---GTAGCTCTGCTACTTCTGGTATTATATGCCAATAATCCAAGT------GGTAGGTGGACTCAAGTGTGATAATCAAGGATAACG-----------------------------------------------------------------------T----GCTCAAGTGTTGTGCTT--------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

droWil2

TGTAGGA---GTAGCTTGTCTACTTCTGGTCCTCAGTGCCTTCAATCCAACT------GGTAGGT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
............AGCTTGTCTACTTCTGGTCCTCAGTGC............................................................................................................................................................................................................................................................................................................	27	1	13.00	13	0	7	0	6	0
............AGCTTGTCTACTTCTGGTCCTCAGTG.............................................................................................................................................................................................................................................................................................................	26	1	4.00	4	0	2	1	1	0
............AGCTTGTCTACTTCTGGTCCTCA................................................................................................................................................................................................................................................................................................................	23	1	2.00	2	0	2	0	0	0
............AGCTTGTCTACTTCTGGTC....................................................................................................................................................................................................................................................................................................................	19	1	2.00	2	0	1	0	1	0
...................CTACTTCTGGTCCTCAGT..............................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	0	1	0
.....................ACTTCTGGTCCTCAGTGCCTTC........................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	1	0
..............CTTGTCTACTTCTGGTCCTCAGTG.............................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	1	0
.............GCTTGTCTACTTCTGGTCCTC.................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	1	0
.............GCTTGTCTACTTCTGGTC....................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	1	0	0	0
..............CTTGTCTACTTCTGGTCCTCAGTGC............................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	1	0
..............CTTGTCTACTTCTGGTCCTCAGT..............................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	1	0
............AGCTTGTCTACTTCTGGTCCTC.................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	1	0	0
.................GTCTACTTCTGGTCCTCAGTGC............................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	1	0
............AGCTTGTCTACTTCTGGT.....................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	1	0	0	0
............AGCTTGTCTACTTCTGGTCCTCAGT..............................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	1	0	0	0
.................................CAGTGCCTTCAATCCAACT------.........................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	1	0

dp5

TGCAGGA---GCAGCCCTGCTGCTTCTTGCACTAAGTGCAAATCAAACCAGT------G----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
............AGCCCTGCTGCTTCTTGCACTAAGC..............................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	1	0	0	0	0	0	0	0
..............................ACTAAGTGCAAATCAAACCAGT------.........................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0
................CTGCTGCTTCTTGCACTAAGTGC............................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	1	0	0	0	0	0	0	0	0
............AGCCCTGCTGCTTCTTGCACTAAGTGC............................................................................................................................................................................................................................................................................................................	27	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..........GCAGCCCTGCTGCTTCTTGCACTAA................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0
............AGCCCTGCTGCTTCTTGCACTAAGTGCA...........................................................................................................................................................................................................................................................................................................	28	1	1.00	1	0	1	0	0	0	0	0	0	0	0

droPer2

TGCAGGC---GCAGCCCTGCTGCTTCTTGCACTAAGTGCAAATCAATCCAGTGCCAATGGTAGGTGGGCCCAAG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
No Reads

droAna3

AGCAGGA---GTAGCCCTTCTACTTCTAGTGCTAAATACAAATTCCATAAGT------GGTAGGTGCTCCCGAGAAAGAGGATCAAGGAT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
..........GTAGCCCTTCTACTTCTAGTG....................................................................................................................................................................................................................................................................................................................	21	1	2.00	2	2	0	0	0	0	0
.......................TTCTAGTGCTAAATACAAATTCCATAAGC------.........................................................................................................................................................................................................................................................................................	29	1	1.00	1	0	1	0	0	0	0
..........GTAGCCCTTCTACTTCTAGTGC...................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	1	0	0	0	0
..................................................................CTCCCGAGAAAGAGGATCAAGGA..........................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	1	0
..........GTAGCCCTTCTACTTCTAGT.....................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0
...................................ATACAAATTCCATAAGT------GAA......................................................................................................................................................................................................................................................................................	20	5	0.20	1	0	1	0	0	0	0

droBip1

AACAGGA---GTAGCCCTGCTACTTCTAGTGCTTAACACAAATTCCATAAGT------GGTAGGTGTACCCGAGAAAGAGAATCAAGGATATA------TCCA---------------------------------TTC--------------------------TCCCAAATCAAGTGCTGA-----AAAC------------------------------------------------------------------------------TAA--GATATT----------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droKik1

AGCAGGA---GTAGCCTTGCTACTTCTTGTGCTAA------GCCCAGCGAGT------GGTAGGTGTTGCC---------GATCAAGGATAAAGGATAATACCAAGTGAAAGTGAAATATAAACACAAAACGGTCATTCTTAAACTGAAATCTTCAAGGATAATCAGAAAATAAAAGTGAA------------------------------------------------------------AATATAAACTATAAAACAGTAAAAATTGC--------------------TTCAAAAAATTATATAAAT------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droFic1

GGCAGGA---GTAGCACTGCTACTTCTCGTGTTAA------ATCATTCAAGT------GGTAGGT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEle1

TGCAGGA---GTAGCCCTGCTACTTCTTGTGATAA------ATCAGTCAAGT------GGTAGGTGTT----TGTC--TAAACCAAGGACGAAGGATAATCCAAAGTTAAAGTGGT------------------CG-----------------------------TGATAGTTCAAGTGCG--------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droBia1

AGCAGGA---GTAGCCCTGCTACTTCTAGTGCTGA------ATCAGTCAAGT------GGTAGGTTTT--CGAGC---CAAGTCAAGGATAA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droTak1

AGCAGGA---GTAGCCCTGCTACTTCTAGTGCTAA------ACCAGTCAAGT------GGTAGGTTGAA-CAAGC---ACCATCAAGGATAAAGGATAATCCAAAGTGAAAGTGAA------------------AG-----------------------------TG-------------------------------GACTACCACCAAAAAAGTGCCA---------------------AATATGAACAA-AAAACAGAAGAA---GCTAA--GAAATTTTAAAGAAACTAACCAAGTTATATAAA-------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEug1

AGCAGGA---GTAGCCTTGCTACTTCTAGTTTTAA------ATCACTCAAGT------GGTAGGT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droYak3

GGCAGGA---GTAGCTCTGCTACTCCTTGTGCTAA------ATCACTCAAGT------GGTAGGTTC----CAGTC--GAAATCCAGGATTAAGATTAG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M026 Head	M043 Female-body	M056 Embryo	V046 Embryo	V052 Head	V058 Head	V120 Male-body	SRR1275488 Male larvae	SRR1275486 Male prepupae	SRR1275484 Male prepupae	GSM1528802 follicle cells
............AGCTCTGCTACTCCTTGTGCT..................................................................................................................................................................................................................................................................................................................	21	1	12.00	12	0	10	2	0	0	0	0	0	0	0	0
............AGCTCTGCTACTCCTTGTGC...................................................................................................................................................................................................................................................................................................................	20	1	11.00	11	0	4	7	0	0	0	0	0	0	0	0
............AGCTCTGCTACTCCTTGTGCTAA------ATC.......................................................................................................................................................................................................................................................................................................	26	1	8.00	8	0	4	4	0	0	0	0	0	0	0	0
............AGCTCTGCTACTCCTTGTGCTAA------ATCA......................................................................................................................................................................................................................................................................................................	27	1	8.00	8	0	7	1	0	0	0	0	0	0	0	0
............AGCTCTGCTACTCCTTGTG....................................................................................................................................................................................................................................................................................................................	19	1	8.00	8	0	8	0	0	0	0	0	0	0	0	0
............AGCTCTGCTACTCCTTGTGCTAA------..........................................................................................................................................................................................................................................................................................................	23	1	8.00	8	0	7	1	0	0	0	0	0	0	0	0
............AGCTCTGCTACTCCTTGTGCTAA------AT........................................................................................................................................................................................................................................................................................................	25	1	6.00	6	0	6	0	0	0	0	0	0	0	0	0
............AGCTCTGCTACTCCTTGTGCTA.................................................................................................................................................................................................................................................................................................................	22	1	5.00	5	0	5	0	0	0	0	0	0	0	0	0
.............GCTCTGCTACTCCTTGTGCTAA------AT........................................................................................................................................................................................................................................................................................................	24	1	3.00	3	0	3	0	0	0	0	0	0	0	0	0
............AGCTCTGCTACTCCTTGTGCTAA------ATCACT....................................................................................................................................................................................................................................................................................................	29	1	3.00	3	0	2	1	0	0	0	0	0	0	0	0
.............GCTCTGCTACTCCTTGTGCTAA------ATC.......................................................................................................................................................................................................................................................................................................	25	1	3.00	3	0	2	1	0	0	0	0	0	0	0	0
....................TACTCCTTGTGCTAA------ATCACTCAAGT------.........................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
..............CTCTGCTACTCCTTGTGCTAA------AT........................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
............................GTGCTAA------ATCACTCAAGT------GA.......................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
...........TAGCTCTGCTACTCCTTGTGCT..................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
.............GCTCTGCTACTCCTTGTGCC..................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0
...........TAGCTCTGCTACTCCTTGT.....................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
..........GTAGCTCTGCTACTCCTTGTGC...................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0
..........GTAGCTCTGCTACTCCTTGT.....................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
...........................TGTGCTAA------ATCACTCAAGT------GA.......................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
..........GTAGCTCTGCTACTCCTTGTGCTAA------ATC.......................................................................................................................................................................................................................................................................................................	28	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0
..............CTCTGCTACTCCTTGTGCT..................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
.............GCTCTGCTACTCCTTGTGCT..................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0
..........GTAGCTCTGCTACTCCTTGTGCTAA------ATCA......................................................................................................................................................................................................................................................................................................	29	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0

droEre2

GGCAGGA---GTAGCTCTGCTACTTCTTGTGCTAA------ATCACTCAAGT------GGTAGGTT----CAAGTG--TAAATCAAGGAT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
............AGCTCTGCTACTTCTTGTGCTAA------AT........................................................................................................................................................................................................................................................................................................	25	1	3.00	3	3	0	0	0	0	0	0
............AGCTCTGCTACTTCTTGTG....................................................................................................................................................................................................................................................................................................................	19	1	3.00	3	3	0	0	0	0	0	0
............AGCTCTGCTACTTCTTGTGCTAA------ATCA......................................................................................................................................................................................................................................................................................................	27	1	2.00	2	2	0	0	0	0	0	0
............AGCTCTGCTACTTCTTGTGC...................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0
.............GCTCTGCTACTTCTTGTGCT..................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	1	0	0	0
..........GTAGCTCTGCTACTTCTTGTG....................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0	0
......................CTTCTTGTGCTAA------ATCACTCAAG................................................................................................................................................................................................................................................................................................	23	1	1.00	1	1	0	0	0	0	0	0

dvi_5936

scaffold_12875:10278045-10278195 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-13.6	-13.6	-13.6