ID:

dvi_429

 Coordinate:

scaffold_13049:22743190-22743250 +

 Confidence:

candidate

 Class:

Canonical miRNA

 Genomic Locale:

intergenic

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-34.4

Flybase annnotation

intergenic

No Repeatable elements found

Sense Strand Reads

ACACTTTTTATTGTCATAATACAAAAATTTTGTCATAACACCGAGTTAATATTACTATCGGTAGTATCGATAATGGTAATCTAAAATATCGAAACTATCGATAGTTTTTTAGCTCGTGATAATGATTCAAGTTTCGTGATCGATTATATCAACTTTCACTT

***********************************...(((.(((((((....((((((((((((.((((((.(((....)))...)))))).))))))))))))...)))))))))).......************************************ 		Read size # Mismatch Hit Count Total Norm Total M047

female body

 V116

male body		 SRR2096056

Ovary_Min_46		 SRR2096060

Ovary_Min_193		 SRR2096059

Ovary_Min_164		 SRR060678

9x140_testes_total

 SRR060656

9x160_ovaries_total

 SRR060677

Argx9_ovaries_total

 V053

head

 SRR060655

9x160_testes_total

 SRR060673

9_ovaries_total

 SRR060680

 9xArg_testes_total

 SRR2096014

Ovary_Strain_160_oxidized		 SRR060672

9x160_females_carcasses_total

 SRR060679

140x9_testes_total

 SRR060689

160x9_testes_total

 SRR060659

Argentina_testes_total

 SRR060674

9x140_ovaries_total

 SRR2096057

Ovary_Min_50		 M027

male body		 SRR060670

9_testes_total

 SRR060676

9xArg_ovaries_total

 SRR060681

Argx9_testes_total

 SRR060683

160_testes_total

 SRR2096013

Ovary_Strain_9_oxidized		 SRR2096055

Ovary_Min_43		 SRR060654

160x9_ovaries_total

 SRR060682

9x140_0-2h_embryos_total

 SRR060684

140x9_0-2h_embryos_total

 SRR060686

Argx9_0-2h_embryos_total

 SRR2096038

embryo_0-2h from 14 day old Strain 9 mothers		 SRR2096058

Ovary_Min_98		 SRR2096072

embryo_0-2h from 15to16 day old dysgenic mothers		 SRR060668

160x9_males_carcasses_total

 V047

embryo

 GSM1528803

follicle cells		 SRR1106729

mixed whole adult body
.................................................TATTACTATCGGTAGTATCGAT.......................................................................................... 22 0 1 63.00 63 5 5 4 8 2 3 2 3 2 3 2 2 0 1 4 2 1 0 3 0 1 2 2 1 1 1 0 1 0 0 1 1 0 0 0 0 0
.................................................TATTACTATCGGTAGTATCGA........................................................................................... 21 0 1 16.00 16 1 2 5 0 1 0 0 0 1 1 0 1 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
.................................................TATTACTATCGGTAGTATCGATA......................................................................................... 23 0 1 14.00 14 2 2 0 0 2 0 0 3 1 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
..................................................ATTACTATCGGTAGTATCGATA......................................................................................... 22 0 1 13.00 13 7 2 0 0 1 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
..................................................ATTACTATCGGTAGTATCGAT.......................................................................................... 21 0 1 12.00 12 0 3 1 1 1 1 2 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.................................................TATTACTATCGGTAGTATCG............................................................................................ 20 0 1 9.00 9 2 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 2 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
..................................................ATTACTATCGGTAGTATCGA........................................................................................... 20 0 1 4.00 4 1 0 0 0 0 0 1 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
................................................ATATTACTATCGGTAGTATCGATC......................................................................................... 24 1 1 2.00 2 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.................................................TATTACTATCGGTAGTATCGATT......................................................................................... 23 1 1 2.00 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
........................................................................................TCGAAACTATCGATAGTTTTTT................................................... 22 0 1 1.00 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.................................................TATTACTATCGGCAGTATCGAT.......................................................................................... 22 1 1 1.00 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..................................................ATTACTATCGGTAGTATCGC........................................................................................... 20 1 1 1.00 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..................................................ATTACTATCGGTAGTATCGATCAA....................................................................................... 24 2 1 1.00 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
..................................................ATTACTATCGGTAGTATCGCT.......................................................................................... 21 1 1 1.00 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
........................................................................................TCGAAACTATCGATAGTTTTT.................................................... 21 0 1 1.00 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.................................................TATTACTATCGGTAGTATAGAT.......................................................................................... 22 1 1 1.00 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..................................................ATTACTATCGGTAGTATCGATAA........................................................................................ 23 0 1 1.00 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
.................................................TATTACTATCGGCAGTATCGA........................................................................................... 21 1 1 1.00 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.................................................TATTACTATCGGTACTATCG............................................................................................ 20 1 1 1.00 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.................................................TAGTACTATCGCTAGTATCG............................................................................................ 20 2 1 1.00 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.................................................TATTACTATCGGTAGTATCGAC.......................................................................................... 22 1 1 1.00 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
........................................................................................TCGAAACTATCGATAGTTTT..................................................... 20 0 2 0.50 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..........................ATTTGGTCATCAAACCGAGTT.................................................................................................................. 21 3 3 0.33 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
..........................................................................................CAAACTATCCCTAGTTTTTTA.................................................. 21 3 6 0.17 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
.........................................................................................................................ATGATGCAAGTTTCTTGAT..................... 19 2 20 0.10 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0
.......................................................TATTGGTAGTAGCTATAATG...................................................................................... 20 3 15 0.07 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
................................................................TACGGACAATGGTAATCT............................................................................... 18 3 20 0.05 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
................................................................TATCGTTAATGGTACT................................................................................. 16 2 20 0.05 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1

Anti-sense strand reads

TGTGAAAAATAACAGTATTATGTTTTTAAAACAGTATTGTGGCTCAATTATAATGATAGCCATCATAGCTATTACCATTAGATTTTATAGCTTTGATAGCTATCAAAAAATCGAGCACTATTACTAAGTTCAAAGCACTAGCTAATATAGTTGAAAGTGAA

************************************...(((.(((((((....((((((((((((.((((((.(((....)))...)))))).))))))))))))...)))))))))).......*********************************** 		Read size # Mismatch Hit Count Total Norm Total SRR060679

140x9_testes_total

 SRR060681

Argx9_testes_total

 SRR060659

Argentina_testes_total

 SRR060655

9x160_testes_total

 M047

female body

 SRR060660

Argentina_ovaries_total

 SRR2096059

Ovary_Min_164		 GSM1528803

follicle cells		 SRR060686

Argx9_0-2h_embryos_total
........................................................................................AGCTTTGATAGCTATCAAAA..................................................... 20 0 2 1.50 3 1 1 0 0 0 1 0 0 0
.....................................................TGATAGCCATCATAGCTAT......................................................................................... 19 0 1 1.00 1 0 0 1 0 0 0 0 0 0
.......................................................................................TAGCTTTGATAGCTATCAAAAAATCG................................................ 26 0 1 1.00 1 0 0 0 1 0 0 0 0 0
.....................................................TGATAGCCATCATAGCTATTA....................................................................................... 21 0 1 1.00 1 0 1 0 0 0 0 0 0 0
........................................................TAGCCATCATAGCTATTACCGT................................................................................... 22 1 1 1.00 1 1 0 0 0 0 0 0 0 0
......................................................................................ATAGCTTTGATAGCTATCAAAA..................................................... 22 0 2 0.50 1 0 0 0 0 1 0 0 0 0
........................................................................................AGCTTTGATAGCTATCAA....................................................... 18 0 4 0.25 1 0 0 1 0 0 0 0 0 0
...........................AAAAAACTATTATGGCTCAAT................................................................................................................. 21 3 7 0.14 1 0 0 0 0 0 0 1 0 0
......................................................GATAGCCATCATCGC............................................................................................ 15 1 9 0.11 1 0 0 0 0 0 0 0 1 0
..................................................................................TTTTCTAGCTTCGATATCTA........................................................... 20 3 20 0.05 1 0 0 0 0 0 0 0 0 1

Re-alignment of all predicted orthologs

Species Coordinate ID Type Alignment
droVir3 scaffold_13049:22743140-22743300 + dvi_429 candidate ACACTTTTTATTGTCATAATA------------CAAAAATTTTGTCATAACACCGAGTTAATATTACTATCGGTAGTATCGATAATGGTAATCTAAAATATCGAAACTATCGATAGTTTTTTAGCTCGTGATAATG-ATTCAAGTTTCGTG----ATCGATTATATCAACTTTCACTT
droMoj3 scaffold_6680:17363174-17363333 + dmo_3131 candidate AAACAATTTATTTTTGAACTATAC------------A-AT---TTTGTAAAACGGAGATATTGCT-CTATCGACAGATTTGATATGGTTGAGCGAAACTATCAAAACTACCGATAGGTTTCTAGCTCTGTAACATG-GTAGACGGAATCTGATATATATATTTAAACCTATTTCACTT
droGri2 scaffold_15110:20785675-20785836 - ACTTTTTGACTTG--------TACTTAAAGTTATAAAACAGGGCTTATAAAACTGGGTTAATACTTCTA----TAGTATTATTGCGCATAGGTTTGTCATTCGAAACAATCAATAGTTTTTTAGCTCTGGTTAGTTTATTTCATTTTC----CATCCTAATCAACTTCTTTTGCACTC

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droVir3
ACACTTTTTATTGTCATAATA------------CAAAAATTTTGTCATAACACCGAGTTAATATTACTATCGGTAGTATCGATAATGGTAATCTAAAATATCGAAACTATCGATAGTTTTTTAGCTCGTGATAATG-ATTCAAGTTTCGTG----ATCGATTATATCAACTTTCACTTSizeHit CountTotal NormTotalM027

Male-body		M028

Head		M047

Female-body		M061

Embryo		SRR060654

Ovary		SRR060655

Testis		SRR060656

Ovary		SRR060657

Testis		SRR060658

Ovary		SRR060659

Testis		SRR060660

Ovary		SRR060661

Embryo		SRR060662

Embryo		SRR060663

Embryo		SRR060664

Male-body		SRR060665

Female-body		SRR060666

Male-body		SRR060667

Female-body		SRR060668

Male-body		SRR060669

Female-body		SRR060670

Testis		SRR060671

Male-body		SRR060672

Female-body		SRR060673

Ovary		SRR060674

Ovary		SRR060675

Ovary		SRR060676

Ovary		SRR060677

Ovary		SRR060678

Testis		SRR060679

Testis		SRR060680

Testis		SRR060681

Testis		SRR060682

Embryo		SRR060683

Testis		SRR060684

Embryo		SRR060685

Embryo		SRR060686

Embryo		SRR060687

Embryo		SRR060688

Ovary		SRR060689

Testis		SRR1106712

embryo_0-2h		SRR1106713

embryo_0-2h		SRR1106714

embryo_2-4h		SRR1106715

embryo_4-6h		SRR1106716

embryo_4-6h		SRR1106717

embryo_6-8h		SRR1106718

embryo_6-8h		SRR1106719

embryo_8-10h		SRR1106720

embryo_8-10h		SRR1106721

embryo_10-12h		SRR1106722

embryo_10-12h		SRR1106723

embryo_12-14h		SRR1106724

embryo_12-14h		SRR1106725

embryo_14-16h		SRR1106726

embryo_14-16h		SRR1106727

larvae		SRR1106728

larvae		SRR1106729

mixed whole adult body		SRR1106730

embryo_16-30h		V047

Embryo		V053

Head		V116

Male-body		GSM1528803

follicle cells		SRR2096013

Ovary_Strain_9_oxidized		SRR2096014

Ovary_Strain_160_oxidized		SRR2096026

embryo_0-2h from 14 day old Strain 9 mothers		SRR2096038

embryo_0-2h from 14 day old Strain 9 mothers		SRR2096050

embryo_0-2h from 14 day old Strain 160 mothers		SRR2096055

Ovary_Min_43		SRR2096056

Ovary_Min_46		SRR2096057

Ovary_Min_50		SRR2096058

Ovary_Min_98		SRR2096059

Ovary_Min_164		SRR2096060

Ovary_Min_193		SRR2096061

embryo_0-2h from 14 day old Strain 160 mothers		SRR2096072

embryo_0-2h from 15to16 day old dysgenic mothers		SRR2096079

embryo_0-2h from 15to16 day old dysgenic mothers		SRR2096080

embryo_0-2h from 15to16 day old nondysgenic mothers		SRR2096081

embryo_0-2h from 15to16 day old nondysgenic mothers
.............................................................TATTACTATCGGTAGTATCGAT...............................................................................................22163.00630050032001000000000010120023342211000002000000000000000000002501001014312800000
.............................................................TATTACTATCGGTAGTATCGA................................................................................................21116.00160010010000000000000000101000001000000000000000000000000000001200100005001001000
.............................................................TATTACTATCGGTAGTATCGATA..............................................................................................23114.00140020000000000000000010010003001000100000000000000000000000001200000000002000000
..............................................................ATTACTATCGGTAGTATCGATA..............................................................................................22113.00130070100000000000000000100000001000000000000000000000000000000200000000001000000
..............................................................ATTACTATCGGTAGTATCGAT...............................................................................................21112.00120000002000000000000000010000100000000000000000000000000000000300200001001100000
.............................................................TATTACTATCGGTAGTATCG.................................................................................................2019.0091020000002000000000000001000000001000001000000000000000000001000000000000000000
..............................................................ATTACTATCGGTAGTATCGA................................................................................................2014.0040010001000000000000000000000000000000000000000000000000000000000200000000000000
............................................................ATATTACTATCGGTAGTATCGATC..............................................................................................2412.0020000000000000000000000000000200000000000000000000000000000000000000000000000000
.............................................................TATTACTATCGGTAGTATCGATT..............................................................................................2312.0020010000000000000000000000000000000000000000000000000000000000000000010000000000
....................................................................................................TCGAAACTATCGATAGTTTTTT........................................................2211.0010000000000000000000000100000000000000000000000000000000000000000000000000000000
..............................................................ATTACTATCGGTAGTATCGC................................................................................................2011.0010000001000000000000000000000000000000000000000000000000000000000000000000000000
....................................................................................................TCGAAACTATCGATAGTTTTT.........................................................2111.0010000000000000000000000000000000000000001000000000000000000000000000000000000000
..............................................................ATTACTATCGGTAGTATCGATAA.............................................................................................2311.0010000000000000000000000000000000000000000000000000000000000000001000000000000000
.............................................................TATTACTATCGGTAGTATCGAC...............................................................................................2211.0010000000000000000000000000000000000000000000000000000000000001000000000000000000
....................................................................................................TCGAAACTATCGATAGTTTT..........................................................2020.5010000000000000000000000000000100000000000000000000000000000000000000000000000000
droMoj3
AAACAATTTATTTTTGAACTATAC------------A-AT---TTTGTAAAACGGAGATATTGCT-CTATCGACAGATTTGATATGGTTGAGCGAAACTATCAAAACTACCGATAGGTTTCTAGCTCTGTAACATG-GTAGACGGAATCTGATATATATATTTAAACCTATTTCACTTSizeHit CountTotal NormTotalM046

Female-body		V041

Embryo		V049

Head		V056

Head		M060

Embryo		V110

Male-body
.............................................................TGCT-CTATCGACAGATTTGAT...............................................................................................2114.004001300
.............................................................TGCT-CTATCGACAGATTTGA................................................................................................2013.003100200
.............................................................TGCT-CTATCGACAGATTTG.................................................................................................1912.002200000
.............................................................TGCT-CTATCGACAGATTTGATA..............................................................................................2211.001000100
droGri2
ACTTTTTGACTTG--------TACTTAAAGTTATAAAACAGGGCTTATAAAACTGGGTTAATACTTCTA----TAGTATTATTGCGCATAGGTTTGTCATTCGAAACAATCAATAGTTTTTTAGCTCTGGTTAGTTTATTTCATTTTC----CATCCTAATCAACTTCTTTTGCACTCSizeHit CountTotal NormTotalM041

Female-body		V109

Male-body
No Reads

Generated: 10/20/2015 at 10:31 PM