dvi_21289

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------################################################## AGATGACTGTTGACGAATGGGAGAAGCCAGGACCATCGGAGAGTGACACCCGTCGCGCAACATGTGCACTATACTAAATTTAGATTAGACTAACACCAGTAGTGTAATCAGAATAAGCTTAGTAGTATGTGAGCTGCACTATGGGGATTGAGTATTAACAATTTAGTAATGGATAAGTTAACGAGCAAGCTGAATTTACAGTTGGACCCGGAAATTATTTCAATCCCGTCGGAAGAGGACGCCGGCAGCGT **********************************************************************************((..((((((((.....))))..............))))........))********************************************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR060673 9_ovaries_total	SRR060675 140x9_ovaries_total	M047 female body	V047 embryo	M027 male body	V053 head	SRR1106721 embryo_10-12h	SRR060684 140x9_0-2h_embryos_total	GSM1528803 follicle cells	SRR060662 9x160_0-2h_embryos_total	SRR060671 9x160_males_carcasses_total	SRR060682 9x140_0-2h_embryos_total	SRR060687 9_0-2h_embryos_total	V116 male body
................................................................................................................ATAAGCTTAGTAGTATGTGAG......................................................................................................................	21	0	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0
.GATGACTGTTGACGAATGGGA.....................................................................................................................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................................................................TGTGAGCTGCACTATGGGGAT.......................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................TTAGACTAACACCAGTAGTGT..................................................................................................................................................	21	0	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0
...................................TCGGAGAGTGACACCCGTCGC...................................................................................................................................................................................................	21	0	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0
.........................GTCAAGTCCATCGGAGAG................................................................................................................................................................................................................	18	3	20	0.35	7	0	0	0	0	2	0	0	0	0	1	1	1	1	1
.......................................................................................................................................GCAGTATGGGGATGCAGTAT................................................................................................	20	3	10	0.10	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0
.......................................................................................................GTAATCTGAATAAGC.....................................................................................................................................	15	1	11	0.09	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0
......................................................................................................................................GGCACTATGGGGATTGGCT..................................................................................................	19	3	14	0.07	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0
.................TGGAAGAGGCCAGGACC.........................................................................................................................................................................................................................	17	2	15	0.07	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0
.......................................................................................................................................GGAGTATGGGGATCGAGTA.................................................................................................	19	3	18	0.06	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0
.......................................................................................................................................GAACTATGGGGATAGAGGA.................................................................................................	19	3	20	0.05	1	0	0	0	0	0	1	0	0	0	0	0	0	0	0
...................................TCGGGGACTGACACCCTT......................................................................................................................................................................................................	18	3	20	0.05	1	0	0	0	0	0	1	0	0	0	0	0	0	0	0

TCTACTGACAACTGCTTACCCTCTTCGGTCCTGGTAGCCTCTCACTGTGGGCAGCGCGTTGTACACGTGATATGATTTAAATCTAATCTGATTGTGGTCATCACATTAGTCTTATTCGAATCATCATACACTCGACGTGATACCCCTAACTCATAATTGTTAAATCATTACCTATTCAATTGCTCGTTCGACTTAAATGTCAACCTGGGCCTTTAATAAAGTTAGGGCAGCCTTCTCCTGCGGCCGTCGCA ********************************************************************************************************************((..((((((((.....))))..............))))........))**********************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	GSM1528803 follicle cells	SRR060682 9x140_0-2h_embryos_total	SRR060676 9xArg_ovaries_total	SRR060681 Argx9_testes_total	SRR060685 9xArg_0-2h_embryos_total	V047 embryo	SRR060675 140x9_ovaries_total	SRR060664 9_males_carcasses_total	SRR060666 160_males_carcasses_total
...............TGACCCTCTTCGTTCCTGGTAA......................................................................................................................................................................................................................	22	3	1	2.00	2	2	0	0	0	0	0	0	0	0
.............................................................................................................................................................TGTTAAATCATTACCTATTCAATTGCTC..................................................................	28	0	1	2.00	2	0	0	1	0	1	0	0	0	0
..............................................................................................................................................................................TTCAATTGCTCGTTCGACTTA........................................................	21	0	1	1.00	1	0	1	0	0	0	0	0	0	0
..................................................................................................................................CACGACGTGATACCCTTAA......................................................................................................	19	2	1	1.00	1	0	0	0	1	0	0	0	0	0
..........................................................TTGTACTCGTGATATGAAT..............................................................................................................................................................................	19	2	1	1.00	1	0	0	0	0	0	1	0	0	0
............................................................................................................................................................................TATTCAATTGAGCGTTCCACT..........................................................	21	3	3	0.33	1	0	0	0	0	0	0	1	0	0
...............................................................................................................................ACACCCGAAGTGATACCGC.........................................................................................................	19	3	20	0.05	1	0	0	0	0	0	0	0	1	0
.........................................................................................................................................TGATACCCTTAGCTCAAAA...............................................................................................	19	3	20	0.05	1	0	1	0	0	0	0	0	0	0
...............................................................................................................TTAATGGAGTCATCATACA.........................................................................................................................	19	3	20	0.05	1	0	0	0	0	0	0	0	0	1

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droVir3	scaffold_13050:304170-304420 -	dvi_21289	AGATGACTGTTGACG---AATGGGAGAAGCCAGGACCATCGGAGAGTGACACCCGTCGCGC-----AACATGTGCAC-------------------TA-TACTAAAT-----TTAGATTAGACTAACACCA---GTAGTGTAATCA--GAATAAG-CTTAGTAGTATGTGAGCTGCACTAT-GGG------GATTGAGTATTAACAATTTAGTAATGGATAAGTTAACGAGCAAGCTGAATTTACAGTTGGACCCGGAAATTATTTCAATCCCGTCGGAAGAGGACGCCGGCAGCGT
droMoj3	scaffold_6093:2095-2149 -		ACGGCGACGAGAGCG-----------AG-------------------------------GAAGAGCGGGACGAA-GGCCGAGGGACAATCCCTGAG------------------------------------------------------------------------------------------------CA-------------------------------------------------------------------------------------------------------
dp5	Unknown_singleton_887:1604-1617 +		CGACCATCGACGAA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droPer2	scaffold_4454:1616-1644 +		CGACCATCGACGAAG---AT---G--ACGAAAGGACC--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droKik1	scf7180000302624:6139-6188 +		AGATGAC-GCCGACAC------CG--AAGCCGGAACCC----------------------ACCAGGAAGACACAGAC-------------------GC----------------------------------------------------------------------------------------------CG-------------------------------------------------------------------------------------------------------
droFic1	scf7180000453385:13938-14077 -		CGGACACCCCCGATAC---GTTTA--ATGACAGCATCGCCGGGCCGATCGACCCGGCGCGGTAAGCAACCAGAA-CC-------------------ATGAAATTAAGTTAAGATCCA---------CACTT---GTAATAGGTTAA--GGT--TAAAAGAGTCCTTTGTTAGCTACACCGT--------------------------------------------------------------------------------------------------------------------
droEle1	scf7180000488056:11255-11384 +		AAGCCACTCCAGACAG---GATCG--ATGACAGCGTCGCCGGACTGAGTAACCCGACACAGTAAGCAGCATGCAGCC-------------------GC-AGATAAATTTAATATTCATTGTATAACCAC--CGTA-----TAACCATTGTATA----------------------------------------------------AGCCTCGTA-----------------------------------------------------------------------------------
droRho1	scf7180000765460:558-735 +		AGATGACGGACTACC-----------CA------------GGGA--GGCGACACGAGAGGTTAAGCAGGGTAGAAGGTCACCCGGTAATTTCTAAG----------------TTGAATTGTACTAACAC--CCTATGTTATAATCT--AAGTACT-CTAAGTAGAGGTTTCACTGCACCGATTGGTAGGGAGCTTAAACAATAAGGGTCTCGTA-TCTATAAGTT------------------------------------------------------------------------
droBia1	scf7180000299209:4763-4949 +		AAATGCA-GCTGCAAAAG-AA--T--ATGACAAGACTACTGGGA--GGCGACACGAGAGGTTAAGCAGGGTAGAGGCTCACACGGTAATTTCTAAG----------------TTGAATTATACTAATACATACAATGTTATTATGT--AAGTATT-CTAAGTCGAGGTTTCACTGCACCGATAGGTAGGGAGCTTGAATAATAAGGGTCTCGTA-----------------------------------------------------------------------------------
droTak1	scf7180000413188:1161-1394 -		CGGACACCGCCGACCC---GATCG--AGGACAGCACCACCGGGCCAAATAGCCCGGCACGGTAAGCATCTGGAA-CC-------------------ACGGAATAACTTTAAGATCCA---------TGCTT---GTAATAGGTTAA--GAT--TA-ATGAGTCCTTTGTTAGCTACACCGTTAGGTAGGAAGGTCGGAAGGTGGGGTTA---TA-TCAATAAGTC------TAGTTTGCTATTTCAGATGGACGAGGTAATCGT---------GTTGTCGGACGACTCCGACGGC--
droEug1	scf7180000409063:12793-12831 +		GGATGAC-GACGACG---ACCACGACATGCCGGGGACACCAGA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droYak3	v2_chrUn_645:35556-35603 -		AGGAGAATGGTGACG---AGAACGTGGAGTCGGATCCATCGGA-----------------------------------------------------------------------------------------------------------------------------------------------AGGAAGCA-------------------------------------------------------------------------------------------------------
droEre2	scaffold_4929:22017929-22017955 -		TGACGAC-GAAGACC-----------ATGAGAAGAGC-----------------------------------------------------------------------------------------------------------------------------------------------------------CA-------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droVir3

AGATGACTGTTGACG---AATGGGAGAAGCCAGGACCATCGGAGAGTGACACCCGTCGCGC-----AACATGTGCAC-------------------TA-TACTAAAT-----TTAGATTAGACTAACACCA---GTAGTGTAATCA--GAATAAG-CTTAGTAGTATGTGAGCTGCACTAT-GGG------GATTGAGTATTAACAATTTAGTAATGGATAAGTTAACGAGCAAGCTGAATTTACAGTTGGACCCGGAAATTATTTCAATCCCGTCGGAAGAGGACGCCGGCAGCGT	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
......................................................................................................................................................ATAAG-CTTAGTAGTATGTGAG.............................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.GATGACTGTTGACG---AATGGGA................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................................................................................................................TGTGAGCTGCACTAT-GGG------GAT.......................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................TTAGACTAACACCA---GTAGTGT............................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................TCGGAGAGTGACACCCGTCGC..............................................................................................................................................................................................................................................	21	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droMoj3

ACGGCGACGAGAGCG-----------AG-------------------------------GAAGAGCGGGACGAA-GGCCGAGGGACAATCCCTGAG------------------------------------------------------------------------------------------------CA-------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
No Reads

dp5

CGACCATCGACGAA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Size

Hit Count

Total Norm

Total

M022
Male-body

M040
Female-body

M059
Embryo

M062
Head

V043
Embryo

V051
Head

V112
Male-body

SRR902010
Ovary

SRR902011
Testis

SRR902012
CNS imaginal disc

No Reads

droPer2

CGACCATCGACGAAG---AT---G--ACGAAAGGACC--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
No Reads

droKik1

AGATGAC-GCCGACAC------CG--AAGCCGGAACCC----------------------ACCAGGAAGACACAGAC-------------------GC----------------------------------------------------------------------------------------------CG-------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droFic1

CGGACACCCCCGATAC---GTTTA--ATGACAGCATCGCCGGGCCGATCGACCCGGCGCGGTAAGCAACCAGAA-CC-------------------ATGAAATTAAGTTAAGATCCA---------CACTT---GTAATAGGTTAA--GGT--TAAAAGAGTCCTTTGTTAGCTACACCGT--------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEle1

AAGCCACTCCAGACAG---GATCG--ATGACAGCGTCGCCGGACTGAGTAACCCGACACAGTAAGCAGCATGCAGCC-------------------GC-AGATAAATTTAATATTCATTGTATAACCAC--CGTA-----TAACCATTGTATA----------------------------------------------------AGCCTCGTA-----------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droRho1

AGATGACGGACTACC-----------CA------------GGGA--GGCGACACGAGAGGTTAAGCAGGGTAGAAGGTCACCCGGTAATTTCTAAG----------------TTGAATTGTACTAACAC--CCTATGTTATAATCT--AAGTACT-CTAAGTAGAGGTTTCACTGCACCGATTGGTAGGGAGCTTAAACAATAAGGGTCTCGTA-TCTATAAGTT------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droBia1

AAATGCA-GCTGCAAAAG-AA--T--ATGACAAGACTACTGGGA--GGCGACACGAGAGGTTAAGCAGGGTAGAGGCTCACACGGTAATTTCTAAG----------------TTGAATTATACTAATACATACAATGTTATTATGT--AAGTATT-CTAAGTCGAGGTTTCACTGCACCGATAGGTAGGGAGCTTGAATAATAAGGGTCTCGTA-----------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droTak1

CGGACACCGCCGACCC---GATCG--AGGACAGCACCACCGGGCCAAATAGCCCGGCACGGTAAGCATCTGGAA-CC-------------------ACGGAATAACTTTAAGATCCA---------TGCTT---GTAATAGGTTAA--GAT--TA-ATGAGTCCTTTGTTAGCTACACCGTTAGGTAGGAAGGTCGGAAGGTGGGGTTA---TA-TCAATAAGTC------TAGTTTGCTATTTCAGATGGACGAGGTAATCGT---------GTTGTCGGACGACTCCGACGGC--	Size	Hit Count	Total Norm	Total
No Reads

droEug1

GGATGAC-GACGACG---ACCACGACATGCCGGGGACACCAGA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droYak3

AGGAGAATGGTGACG---AGAACGTGGAGTCGGATCCATCGGA-----------------------------------------------------------------------------------------------------------------------------------------------AGGAAGCA-------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M026 Head	M043 Female-body	M056 Embryo	V046 Embryo	V052 Head	V058 Head	V120 Male-body	SRR1275488 Male larvae	SRR1275486 Male prepupae	SRR1275484 Male prepupae	GSM1528802 follicle cells
.............CG---AGAACGTGGAGTCGGATCCATC.................................................................................................................................................................................................................................................................	24	2	0.50	1	0	1	0	0	0	0	0	0	0	0	0
........................TGGAGTCGGATCCATCGG...............................................................................................................................................................................................................................................................	18	20	0.05	1	0	1	0	0	0	0	0	0	0	0	0
..................AGAACGTGGAGTCGGATCCAT..................................................................................................................................................................................................................................................................	21	20	0.05	1	0	1	0	0	0	0	0	0	0	0	0
..........................GAGTCGGATCCATCGGA-----------------------------------------------------------------------------------------------------------------------------------------------...............................................................................................................	17	20	0.05	1	0	0	0	0	0	0	0	0	0	0	1

droEre2

TGACGAC-GAAGACC-----------ATGAGAAGAGC-----------------------------------------------------------------------------------------------------------------------------------------------------------CA-------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
No Reads

-3.1	-3.1	-2.8	-2.8

dvi_21289

scaffold_13050:304220-304370 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads