dvi_20783

GAGCTAGCTTTACATGCGTTGCCGGACCCGCTAGTCTATCGCAAGATGACCATTCAAGTTAGCTCAGGAAACGGGTCAACGCATCCAGCGTACTTTTGTTTTCACATTTGCACGGTTCCTATAGCTAAAAAAGGAGTAGATGACGAAGGAGAAACACGAGACGACAATGCTTTGCGTCAGCACTGCAGCGGTCGGGAGCAAGCGGTTGGGCGGTGGATGGATGGGAACCAGCCCGACGTAACGCGTCCGTG **************************************************************************************************************************.(((((((((((.((......((((....(((............)))...)))))).)))))).)))))********************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR060679 140x9_testes_total	M047 female body	SRR060664 9_males_carcasses_total	M027 male body	SRR060663 160_0-2h_embryos_total	V116 male body	M028 head	SRR060669 160x9_females_carcasses_total	M061 embryo	SRR060678 9x140_testes_total	SRR1106730 embryo_16-30h
............................................................................CAACGCATCCAGCGTACTTTT..........................................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0
...............................TAGTCTGTCGCAAGATGGC.........................................................................................................................................................................................................	19	2	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0
...............................TAGTCTGTCGCAAGATGG..........................................................................................................................................................................................................	18	2	3	1.00	3	3	0	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................GCACTGCAGCGGTCGGGAGC....................................................	20	0	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0
.........................................CAAGGAGAGCATTCAAGTTA..............................................................................................................................................................................................	20	3	8	0.50	4	0	0	0	0	0	2	2	0	0	0	0
..........................................................................................................................AGCAAAAAAAGGAATAGATGA............................................................................................................	21	2	2	0.50	1	0	0	0	0	1	0	0	0	0	0	0
.....................................................................................................................................................AGAAACTCGAGAAGACAGTGCT................................................................................	22	3	2	0.50	1	0	0	0	1	0	0	0	0	0	0	0
.....................................................................................................................................................AGAAACTCGAGAAGACAGTGC.................................................................................	21	3	6	0.17	1	0	0	0	0	0	0	0	1	0	0	0
................................................................................................................................................................................................CGGGAGTAAGTGGTTGGGTG.......................................	20	3	12	0.08	1	0	0	0	0	0	0	0	0	1	0	0
.........................................................................................................................................................................................................GCGGTTGGTCGGTCGATG................................	18	2	17	0.06	1	0	0	0	0	0	1	0	0	0	0	0
..................................................................................................................................................................................................GGACCAAGGGGTTGTGCGG......................................	19	3	18	0.06	1	0	0	0	0	0	0	0	0	0	1	0
................................................................................................................................................................................................CAGGACCAAGCGGTTCGG.........................................	18	3	20	0.05	1	0	1	0	0	0	0	0	0	0	0	0
..............................................................CTCATGAAACGGATCAAGG..........................................................................................................................................................................	19	3	20	0.05	1	0	0	0	1	0	0	0	0	0	0	0
...............................................................................................................................................................................................TCAGGACCAAGCGGTT............................................	16	2	20	0.05	1	0	0	0	0	0	0	0	0	0	0	1

Species	Coordinate	ID	Alignment
droVir3	scaffold_13049:20518955-20519205 -	dvi_20783	CTCGATCGAAATGTACGCAACGGCCTGGGCGATCAGATAGCGTTCTACTGGTAAGTTCAAT--C------G-A-----------------------------------------------------------GTCCTTTGCCC------------AGTTGCGTAGGTCGCATGAAAACAAAAGTGTAA-ACGTGCCA---------------------------------------------------------------------AGG--ATATCGATTTTTTCCTCATCTACTGCTTCCTCTTTGTGC---TCTGCTGTTACGAAACGC---AGTCGTGACGTCGCCAGCCC--------------------------------------------------------------TCGTTCGCCAACCCGCCA----CCT-ACCTACCCTTGGTCGGGCTGCATTGCGCAGGCAC---
droGri2	scaffold_15110:11988329-11988696 +		CTAGATCGGAATGTGCGCAACGGACTCGGAGATCAGATAGCTTACTACTGGTAAGTCAAAT--TCAGAATTTT-----------------------------------------------------------AATCCCAAAT-AAATCCATAAACAGTTAGGTAGGTCGCATGAAAACAAAAGTTG------TAGCAGCAACATACTCTCTTTCTCTCCCCTCTCTCTCTCACTATCTCTCTTGCACACACGTACTTT-AAGGATAACAACGTATTGATTTTTATTT-----------------TCGTACTTTTTTGCTATTACGAAACGT---GCTCGTGACGTTGGCGACCTGCCGCAACCCTTCACACCATCTCTCCCCCTCCCCTCTCTCCCCACCGACAGTGCTCCCCACATTGCCTGTAAA--------CTTGGTGAGCT----TTGCAT-------AGTGCGCAGGCAC---
droWil2	scf2_1100000004768:888243-888396 -		TTAGATCGTAATGTACGTAATGGATTGGGCGACCAAATAGCCTATTATTGGTGAGTACAAT--T------AAA---TAAGCAAACAATAACATTCTTCAAAAGGGC-------------------------------------------AAACT---GAGCTTGGGTTGTGTGAGAACAAAAGG-------------------------------------------------------------------------------ATAACA--GTATTGATTTTTCTTT---------------------------------------------------------------------------------------------------------------------------------------------------------------CTTCT----------------------------
dp5	XR_group6:12636312-12636559 -		CTCGATCGCAACGTCCGCAACGGATTGGGCGACCAGATCGCCTACTATTGGTAAGTCCAAA--GCTGTTCTGT---------------------------------------------------------ACTGTCCTTTCCCACTTTCCCATTCGGTTGCGTAGGTCGCATGAAAACAAAAGTTCCA-GG-GGAC-----------------------------------------------------ACGTTCTGAAAAGGACAACA--GTATTGATTTTTCTCT--------------------------TGTGTGGTTACGAAACTCTCGCCTCGTGACGTAGATGG-------------------------------------------------------------------GTATGTGAA-----TATCTAGGT-----------------GATTTACTGCGCAGGCACCAA
droPer2	scaffold_33:657782-658029 -		CTCGATCGCAACGTCCGCAACGGATTGGGCGACCAGATCGCCTACTATTGGTAAGTCCAAA--GCTGTACTGT---------------------------------------------------------ACTGTCCTTTCCCACTTTCCCATTCGGTTGCGTAGGTCGCATGAAAACAAAAGTTCCA-GG-GGAC-----------------------------------------------------ACGTTCTGAAAAGGACAACA--GTATTGATTTTTCTCT--------------------------TGTGTGGTTACGAAACTCTCGCCTCGTGACGTAGATGG-------------------------------------------------------------------GTACGTGAA-----TATCTAGGT-----------------GATTTACTGCGCAGGCACCAA
droAna3	scaffold_13337:8424930-8425091 +		CTGGATCGCAATGTCCGGAACGGATTGGGTGACCAGATTGCCTACTACTGGTGAGTTTACA--GCCT---CGTCAT--------------------------------------------------CGCCGTTGTCCTCGTCAACCACGGCACTGGGTGGTGTAGGTCGAATGAAAACAAAAGTTGCTCGAATGCCAGG----------------------------------------------------------------ACAGGA--GTATTGATTTTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droBip1	scf7180000396541:570586-570742 +		CTGGATCGCAATGTCCGGAACGGATTGGGCGACCAGATTGCCTACTACTGGTGAGTTCTCA--GCCC---C-------------------------------------------------------CGTCGTCGTCCTCGTCAACCGTGGCACTGGGTGGTGTAGGTCGAATGAAAACAAAAGTTGCTCGAGTGTCAGG----------------------------------------------------------------ACAGGA--TTATTGATTTTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droKik1	scf7180000302260:41336-41447 +		CTGGATCGCAATGTCCGCAACGGTCTGGGCGACCAAATAGCCTACTACTGGTGAGTCCTC-------------------------------------------------------------------------CTCCTCGCCCACTCCCCTTGTGGGTATCGTAGGTCGCATGAAAACAAAAGTG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droFic1	scf7180000453929:419905-420128 -		CTGGATCGGAATGTTAGGAACGGCCTGGGCGACCAAATCGCCTACTACTGGTGAGTCTGGC--T------GCT-----------------------------------------------------------AATCCTCGCCCACTTTCCCCCTGGGTGGCGTAGGTCGTACGAAAACAAAAGTTTTT-GGGTGGCAGC----------------------------------------------------------------ACAGGA--TTATTGATTTTTC------------------------------GTGTGGTTACGAAACTTTCGCCTTGTGACGTCGCAA----------------------------------------------------------------------------------------GGTGAGCTCTTCTTGGACAGGACTCACTGCGCAGGCAC---
droEle1	scf7180000491193:2071261-2071437 +		CTGGATCGCAATGTTAGGAATGGTCTGGGCGACCAAATAGCCTACTACTGGTGAGTCTGAC--T------GGC---TG----------------------GCGGGCTGACTGGCTTGCTGGCTGGC------AATCCTCGCCCACTTTCCCACTGGGTGGCGTAGGTCGCATGAAAACAAAAGTTTCT-GGGTGGCAGC----------------------------------------------------------------ACAGGA--TTATTGATTTTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droRho1	scf7180000777887:24386-24534 +		CTGGATCGCAATGTTAGGAACGGTCTGGGCGACCAAATCGCATACTACTGGTGAGTCTGGC--T------GGC-----------------------------------------------------------AATCCTAGCCCACTTTCCCACTGGGTGGCGTAGGTCGCATGAAAACAAAAGTTTTT-GGGTGGCAGC----------------------------------------------------------------ACAGGA--TTATTGATTTTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droBia1	scf7180000302193:4759515-4759731 +		CTGGATCGCAATGTTAGGAATGGTCTGGGTGACCAAATCGCCTACTACTGGTGAGTCTGGC--T------GGC-----------------------------------------------------------AGTCCTCGCCCACTTTCCCTGTGGGTGGCGTAGGTCGCATGAAAACAAAAGTTT-T-GGGTGGC---------------------------------------------------------------------AGGA--TTATTGATTTTTC------------------------------GTGTGGTTACGAAACTCTCGCCTTGTGACGTACCAA----------------------------------------------------------------------------------------GGTG-GCTCTTCTTGGACAGGATTCACTGCGCAGGCAC---
droTak1	scf7180000414337:301126-301262 +		CTGGATCGCAACGTTAGGAACGGACTGGGCGACCAAATCGCCTATTACTGGTGAGTCTGGCCAT------GCT-----------------------------------------------------------AATCCTCGCCCACTTTCCCGCTGGGTGGCGTAGGTCGCATGAAAACAAAAGT-------------------------------------------------------------------------------GCAGGA--TTATTGATTTTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEug1	scf7180000409711:4733773-4733997 +		CTGGATCGCAATGTTAGGAATGGTCTGGGCGACCAAATAGCCTATTACTGGTGAGTCTGAC--T------GCT-----------------------------------------------------------AATCCTCGCCCACTTGCCCACTGGGTGGCGTAGGTCGTATGAAAACAAAAGTTTTTGGGGTGGCAGT----------------------------------------------------------------GCAGGA--TTATTGATTTTTC------------------------------GTGTGGTTACGAAACTCTCGCCTCGTGACGTCGGAA----------------------------------------------------------------------------------------GGTGGGCTCCTCTTTGACAGGATTTACTGCGCAGGCAC---
dm3	chr3L:21272448-21272624 -		CTAGATCGCAACGTTAGGAACGGCCTGGGCGACCAGATAGCCTACTACTGGTGAGTTCCCT--T------GCA-----------------------------------------------------------AATCCTCGCTCACTTTCGCTCTGGGTGGCGTAGGTCGCACGAAAACAAAAGTATTT-GGTTGGCAGC----------------------------------------------------------------GCAGGA--TTATTGATTTTTC------------------------------GCCTGGTTACGAAACTCTCGCCTTGTG------------------------------------------------------------------------------------------------------------------------------------------
droSim2	3l:20815644-20815867 -	dsi_6055	CTAGATCGCAACGTAAGGAACGGCCTGGGCGACCAGATAGCCTACTACTGGTGAGTTCGGT--T------GCA-----------------------------------------------------------AATCCTCGCCCACTTTCGCTCTGGGTGGCGTAGGTCGCACGAAAACAAAAGTATTT-GGGTGGCAGC----------------------------------------------------------------GCAGGA--TTATTGATTTTTC------------------------------GTCTGGTTACGAAACTCTCGCCTTGTGACGTAGAAA----------------------------------------------------------------------------------------GGTGGGCTCCTCTTGCACAGGATTTACTGCGCAGGCAC---
droSec2	scaffold_11:1218682-1218830 -		CTAGATCGCAACGTAAGGAACGGCCTGGGCGACCAGATAGCCTACTACTGGTGAGTTCGGT--T------GCA-----------------------------------------------------------AATCCTCGCCCACTTTCGCTCTGGGTGGCGTAGGTCGTACGAAAACAAAAGTATTT-GTGTGGCAGC----------------------------------------------------------------GCAGGA--TTATTGATTTTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droYak3	3L:19245146-19245294 +		CTGGATCGCAACGTGAGGAACGGTCTGGGCGACCAGATAGCCTACTACTGGTGAGTCTGGT--T------GGA-----------------------------------------------------------AATCCTCGCCCACTTTCCCTGCGGGTGGCGTAGGTCGCACGAAAACAAAAGTATTT-AGGAGGCAGC----------------------------------------------------------------GCAGGA--TTATTGATTTTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEre2	scaffold_4784:20948542-20948690 -		CTAGATCGCAACGTTAGGAACGGTCTGGGCGACCAGATAGCCTACTACTGGTGAGTCTGGT--T------GGA-----------------------------------------------------------AATCCTCGCCCACTTTCCCTCTGGGTGGCGTAGGTCGAACGAAAACAAAAGTATTT-ATGTGGTAGC----------------------------------------------------------------GCAGGA--TTATTGATTTTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droVir3

CTCGATCGAAATGTACGCAACGGCCTGGGCGATCAGATAGCGTTCTACTGGTAAGTTCAAT--C------G-A-----------------------------------------------------------GTCCTTTGCCC------------AGTTGCGTAGGTCGCATGAAAACAAAAGTGTAA-ACGTGCCA---------------------------------------------------------------------AGG--ATATCGATTTTTTCCTCATCTACTGCTTCCTCTTTGTGC---TCTGCTGTTACGAAACGC---AGTCGTGACGTCGCCAGCCC--------------------------------------------------------------TCGTTCGCCAACCCGCCA----CCT-ACCTACCCTTGGTCGGGCTGCATTGCGCAGGCAC---	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
..............ACGCAACGGCCTGGGCGATC.............................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............GTACGCAACGGCCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
................GCAACGGCCTGGGCGATCAGATAG.......................................................................................................................................................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................CAGATAGCGTTCTACTGGG...........................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................................................................................................................................................................................................................................CTTGGTCGGGCTGCATTG............	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....ATCGAAATGTACGCAACGGCCT.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................GGCCTGGGCGATCAGATAGCGTT...................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................AAT--C------G-A-----------------------------------------------------------GTCCTTTGCCC------------AGT.................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
.....................................................................................................................................................................................AGTGTAA-ACGTGCCA---------------------------------------------------------------------AGG--ATATCGAT........................................................................................................................................................................................................	26	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........TGTACGCAACGGCCTGGGCGATCA............................................................................................................................................................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................................................................................................ACTGCTTCCTCTTTGTGC---TCT...................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................AACGGCCTGGGCGATCAGATAGC......................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................................................................................................................................GAAACGC---AGTCGTGACGTCGCCAGC...............................................................................................................................	25	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................GATCAGATAGCGTTCTACTGGG...........................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................GATCAGATAGCGTTCTACTGG............................................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..........ATGTACGCAACGGCCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................TGGGCGATCAGATAGCGTT...................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................................................................................................................................................................................................................................CTACCCTTGGTCGGGT...................	16	2	0.50	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0

droGri2

CTAGATCGGAATGTGCGCAACGGACTCGGAGATCAGATAGCTTACTACTGGTAAGTCAAAT--TCAGAATTTT-----------------------------------------------------------AATCCCAAAT-AAATCCATAAACAGTTAGGTAGGTCGCATGAAAACAAAAGTTG------TAGCAGCAACATACTCTCTTTCTCTCCCCTCTCTCTCTCACTATCTCTCTTGCACACACGTACTTT-AAGGATAACAACGTATTGATTTTTATTT-----------------TCGTACTTTTTTGCTATTACGAAACGT---GCTCGTGACGTTGGCGACCTGCCGCAACCCTTCACACCATCTCTCCCCCTCCCCTCTCTCCCCACCGACAGTGCTCCCCACATTGCCTGTAAA--------CTTGGTGAGCT----TTGCAT-------AGTGCGCAGGCAC---	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

droWil2

TTAGATCGTAATGTACGTAATGGATTGGGCGACCAAATAGCCTATTATTGGTGAGTACAAT--T------AAA---TAAGCAAACAATAACATTCTTCAAAAGGGC-------------------------------------------AAACT---GAGCTTGGGTTGTGTGAGAACAAAAGG-------------------------------------------------------------------------------ATAACA--GTATTGATTTTTCTTT---------------------------------------------------------------------------------------------------------------------------------------------------------------CTTCT----------------------------	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
.........AATGTACGTAATGGATTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	6.00	6	2	0	0	4	0
..................AATGGATTGGGCGACCAAAT.........................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	3.00	3	0	0	1	2	0
.......GTAATGTACGTAATGGATTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	3.00	3	0	0	0	3	0
.........AATGTACGTAATGGATTGGGCGACCA............................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	1	2.00	2	0	0	1	1	0
.........AATGTACGTAATGGATTGGGCGACCAAA..........................................................................................................................................................................................................................................................................................................................................................................................................................................................	28	1	2.00	2	2	0	0	0	0
...............CGTAATGGATTGGGCGACCAAATAGCC.....................................................................................................................................................................................................................................................................................................................................................................................................................................................	27	1	2.00	2	2	0	0	0	0
..................AATGGATTGGGCGACCAAATAGC......................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	2.00	2	0	0	1	1	0
................GTAATGGATTGGGCGACCA............................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	1	0
..............ACGTAATGGATTGGGCGACCAA...........................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	1	0	0	0	0
...................................................................................................................................................................................AAAGG-------------------------------------------------------------------------------ATAACA--GTATTGAT........................................................................................................................................................................................................	19	1	1.00	1	0	0	0	1	0
................GTAATGGATTGGGCGACCAAATAGCC.....................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	1	0	0	0	0
..............ACGTAATGGATTGGGCGACC.............................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0
..............ACGTAATGGATTGGGCGACCAAATAG.......................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	1	0
.......GTAATGTACGTAATGGATTGGG..................................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	1	0
..AGATCGTAATGTACGTAATGGAT......................................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	1	0
TTAGATCGTAATGTACGT.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	0	1	0
..............ACGTAATGGATTGGGCGACCA............................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0
...................ATGGATTGGGCGACCAAATAGC......................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	1	0	0
...........TGTACGTAATGGATTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	1	0
.........................................CTATTATTGGTGAGTACAAT--T------.........................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	1	0
...............CGTAATGGATTGGGCGACCA............................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	1	0	0
......CGTAATGTACGTAATGGATTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	1	0
............GTACGTAATGGATTGGGCG................................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	1	0
...............CGTAATGGATTGGGCGACC.............................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	1	0

dp5

CTCGATCGCAACGTCCGCAACGGATTGGGCGACCAGATCGCCTACTATTGGTAAGTCCAAA--GCTGTTCTGT---------------------------------------------------------ACTGTCCTTTCCCACTTTCCCATTCGGTTGCGTAGGTCGCATGAAAACAAAAGTTCCA-GG-GGAC-----------------------------------------------------ACGTTCTGAAAAGGACAACA--GTATTGATTTTTCTCT--------------------------TGTGTGGTTACGAAACTCTCGCCTCGTGACGTAGATGG-------------------------------------------------------------------GTATGTGAA-----TATCTAGGT-----------------GATTTACTGCGCAGGCACCAA	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
............GTCCGCAACGGATTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	3.00	3	0	3	0	0	0	0	0	0	0	0
...............CGCAACGGATTGGGCGACCAGAT.........................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	2.00	2	0	2	0	0	0	0	0	0	0	0
.....................GGATTGGGCGACCAGATCGC......................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0
......CGCAACGTCCGCAACGGATTGGGCG................................................................................................................................................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...............CGCAACGGATTGGGCGACCA............................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..............................GACCAGATCGCCTACTATT..............................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................................................................................................CT--------------------------TGTGTGGTTACGAAACT.....................................................................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..CGATCGCAACGTCCGCAACGGATTGGG..................................................................................................................................................................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.......GCAACGTCCGCAACGGATTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..CGATCGCAACGTCCGCAACGGATTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	28	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...GATCGCAACGTCCGCAACG.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.................CAACGGATTGGGCGACCAGATCG.......................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................GAC-----------------------------------------------------ACGTTCTGAAAAGGA.......................................................................................................................................................................................................................	18	1	1.00	1	0	0	0	0	1	0	0	0	0	0
.........................................................................................................................................................................................CCA-GG-GGAC-----------------------------------------------------ACGTTCTGAAAAGGACAAA...................................................................................................................................................................................................................	28	1	1.00	1	0	0	0	0	0	0	0	0	1	0
......CGCAACGTCCGCAACGGATTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.....TCGCAACGTCCGCAACGGATTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..............CCGCAACGGATTGGGCGACC.............................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.........AACGTCCGCAACGGATTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...GATCGCAACGTCCGCAACGGATTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	0	1	0	0	0	0	0	0	0	0

droPer2

CTCGATCGCAACGTCCGCAACGGATTGGGCGACCAGATCGCCTACTATTGGTAAGTCCAAA--GCTGTACTGT---------------------------------------------------------ACTGTCCTTTCCCACTTTCCCATTCGGTTGCGTAGGTCGCATGAAAACAAAAGTTCCA-GG-GGAC-----------------------------------------------------ACGTTCTGAAAAGGACAACA--GTATTGATTTTTCTCT--------------------------TGTGTGGTTACGAAACTCTCGCCTCGTGACGTAGATGG-------------------------------------------------------------------GTACGTGAA-----TATCTAGGT-----------------GATTTACTGCGCAGGCACCAA	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
..................AACGGATTGGGCGACCAGATCGC......................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	9.00	9	9	0	0	0	0	0
..............................GACCAGATCGCCTACTATTG.............................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	5.00	5	5	0	0	0	0	0
......CGCAACGTCCGCAACGGATTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	24	1	5.00	5	5	0	0	0	0	0
............GTCCGCAACGGATTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	4.00	4	3	1	0	0	0	0
.........AACGTCCGCAACGGATTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	3.00	3	3	0	0	0	0	0
..............................GACCAGATCGCCTACTAT...............................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	2.00	2	2	0	0	0	0	0
..............CCGCAACGGATTGGGCGACCA............................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
.........AACGTCCGCAACGGATTGGG..................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	1	0	0	0
..CGATCGCAACGTCCGCAACGGATTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	28	1	1.00	1	1	0	0	0	0	0
..............................GACCAGATCGCCTACTATT..............................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	1	0	0	0	0	0
................GCAACGGATTGGGCGACCA............................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	1	0	0	0	0	0
...............CGCAACGGATTGGGCGACCA............................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
..............CCGCAACGGATTGGGCGACC.............................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
.........................................CTACTATTGGTAAGTCCAAA--GCTGTACT........................................................................................................................................................................................................................................................................................................................................................................................................................	28	1	1.00	1	1	0	0	0	0	0
............................................................................................................................................................................................................................................................................................................................................CGCCTCGTGACGTAGATGG-------------------------------------------------------------------GTACGTGG.....................................................	27	1	1.00	1	1	0	0	0	0	0

droAna3

CTGGATCGCAATGTCCGGAACGGATTGGGTGACCAGATTGCCTACTACTGGTGAGTTTACA--GCCT---CGTCAT--------------------------------------------------CGCCGTTGTCCTCGTCAACCACGGCACTGGGTGGTGTAGGTCGAATGAAAACAAAAGTTGCTCGAATGCCAGG----------------------------------------------------------------ACAGGA--GTATTGATTTTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
......CGCAATGTCCGGAACGGATTGGGT.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	24	1	3.00	3	1	0	0	0	2	0
...GATCGCAATGTCCGGAACGGAT......................................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	3.00	3	0	1	0	0	2	0
................GGAACGGATTGGGTGACCAGAT.........................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	2.00	2	0	1	0	0	1	0
...GATCGCAATGTCCGGAACGGATTGGGT.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	27	1	2.00	2	0	1	0	0	1	0
....ATCGCAATGTCCGGAACGGATTG....................................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	2.00	2	0	0	1	0	1	0
................GGAACGGATTGGGTGACCAGATTGC......................................................................................................................................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	1	0	0	0	0
....ATCGCAATGTCCGGAACGG........................................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	1	0	0	0	0	0
...GATCGCAATGTCCGGAACGGA.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0
............GTCCGGAACGGATTGGGT.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	1	0	0	0
.................GAACGGATTGGGTGACCAGATTGCCT....................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	1	0	0	0	0
.....................................................................................................................................................................................AGTTGCTCGAATGCCAGG----------------------------------------------------------------ACAGGA--................................................................................................................................................................................................................	24	1	1.00	1	0	0	1	0	0	0
.........AATGTCCGGAACGGATTGGGT.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0
...................................................................................................................................................................................AAAGTTGCTCGAATGCCAGG----------------------------------------------------------------ACAGGA--................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	1	0
..................................................................................................................................................................................AAAAGTTGCTCGAATGCCAGG----------------------------------------------------------------ACAGG...................................................................................................................................................................................................................	26	1	1.00	1	0	1	0	0	0	0
......CGCAATGTCCGGAACGGATTGG...................................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	1	0	0	0	0	0
...GATCGCAATGTCCGGAACGGATTG....................................................................................................................................................................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	1	0
.....TCGCAATGTCCGGAACGGATT.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
..................AACGGATTGGGTGACCAGAT.........................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	1	0	0	0

droBip1

CTGGATCGCAATGTCCGGAACGGATTGGGCGACCAGATTGCCTACTACTGGTGAGTTCTCA--GCCC---C-------------------------------------------------------CGTCGTCGTCCTCGTCAACCGTGGCACTGGGTGGTGTAGGTCGAATGAAAACAAAAGTTGCTCGAGTGTCAGG----------------------------------------------------------------ACAGGA--TTATTGATTTTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droKik1

CTGGATCGCAATGTCCGCAACGGTCTGGGCGACCAAATAGCCTACTACTGGTGAGTCCTC-------------------------------------------------------------------------CTCCTCGCCCACTCCCCTTGTGGGTATCGTAGGTCGCATGAAAACAAAAGTG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droFic1

CTGGATCGGAATGTTAGGAACGGCCTGGGCGACCAAATCGCCTACTACTGGTGAGTCTGGC--T------GCT-----------------------------------------------------------AATCCTCGCCCACTTTCCCCCTGGGTGGCGTAGGTCGTACGAAAACAAAAGTTTTT-GGGTGGCAGC----------------------------------------------------------------ACAGGA--TTATTGATTTTTC------------------------------GTGTGGTTACGAAACTTTCGCCTTGTGACGTCGCAA----------------------------------------------------------------------------------------GGTGAGCTCTTCTTGGACAGGACTCACTGCGCAGGCAC---	Size	Hit Count	Total Norm	Total
No Reads

droEle1

CTGGATCGCAATGTTAGGAATGGTCTGGGCGACCAAATAGCCTACTACTGGTGAGTCTGAC--T------GGC---TG----------------------GCGGGCTGACTGGCTTGCTGGCTGGC------AATCCTCGCCCACTTTCCCACTGGGTGGCGTAGGTCGCATGAAAACAAAAGTTTCT-GGGTGGCAGC----------------------------------------------------------------ACAGGA--TTATTGATTTTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droRho1

CTGGATCGCAATGTTAGGAACGGTCTGGGCGACCAAATCGCATACTACTGGTGAGTCTGGC--T------GGC-----------------------------------------------------------AATCCTAGCCCACTTTCCCACTGGGTGGCGTAGGTCGCATGAAAACAAAAGTTTTT-GGGTGGCAGC----------------------------------------------------------------ACAGGA--TTATTGATTTTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droBia1

CTGGATCGCAATGTTAGGAATGGTCTGGGTGACCAAATCGCCTACTACTGGTGAGTCTGGC--T------GGC-----------------------------------------------------------AGTCCTCGCCCACTTTCCCTGTGGGTGGCGTAGGTCGCATGAAAACAAAAGTTT-T-GGGTGGC---------------------------------------------------------------------AGGA--TTATTGATTTTTC------------------------------GTGTGGTTACGAAACTCTCGCCTTGTGACGTACCAA----------------------------------------------------------------------------------------GGTG-GCTCTTCTTGGACAGGATTCACTGCGCAGGCAC---	Size	Hit Count	Total Norm	Total
No Reads

droTak1

CTGGATCGCAACGTTAGGAACGGACTGGGCGACCAAATCGCCTATTACTGGTGAGTCTGGCCAT------GCT-----------------------------------------------------------AATCCTCGCCCACTTTCCCGCTGGGTGGCGTAGGTCGCATGAAAACAAAAGT-------------------------------------------------------------------------------GCAGGA--TTATTGATTTTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEug1

CTGGATCGCAATGTTAGGAATGGTCTGGGCGACCAAATAGCCTATTACTGGTGAGTCTGAC--T------GCT-----------------------------------------------------------AATCCTCGCCCACTTGCCCACTGGGTGGCGTAGGTCGTATGAAAACAAAAGTTTTTGGGGTGGCAGT----------------------------------------------------------------GCAGGA--TTATTGATTTTTC------------------------------GTGTGGTTACGAAACTCTCGCCTCGTGACGTCGGAA----------------------------------------------------------------------------------------GGTGGGCTCCTCTTTGACAGGATTTACTGCGCAGGCAC---	Size	Hit Count	Total Norm	Total
No Reads

dm3

CTAGATCGCAACGTTAGGAACGGCCTGGGCGACCAGATAGCCTACTACTGGTGAGTTCCCT--T------GCA-----------------------------------------------------------AATCCTCGCTCACTTTCGCTCTGGGTGGCGTAGGTCGCACGAAAACAAAAGTATTT-GGTTGGCAGC----------------------------------------------------------------GCAGGA--TTATTGATTTTTC------------------------------GCCTGGTTACGAAACTCTCGCCTTGTG------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	GSM154618 Ovary	GSM154620 Ovary	GSM154621 Ovary	GSM154622 Ovary	GSM180328 Head	GSM180329 Mixed-body	GSM180330 Embryo	GSM180331 Embryo	GSM180332 Embryo	GSM180333 Embryo	GSM180334 Larvae	GSM180335 Disc	GSM180336 Pupae	GSM180337 S2	GSM231091 Ovary	GSM239041 Head	GSM239050 Head	GSM239051 S2	GSM239052 S2	GSM246084 Head	GSM266765 S2	GSM280082 Ovary	GSM280083 Ovary	GSM280084 Ovary	GSM280085 Testis	GSM280086 Ovary	GSM280087 S2-NP	GSM280088 S2-NP	GSM312995 Head	GSM313160 Head	GSM313161 Head	GSM313162 Head	GSM313163 Head	GSM313164 Head	GSM313165 Head	GSM343287 Embryo	GSM379050 Ovary	GSM379051 Ovary	GSM379052 Ovary	GSM379053 Ovary	GSM379054 Ovary	GSM379055 Ovary	GSM379056 Ovary	GSM379057 Ovary	GSM379058 Ovary	GSM379059 Ovary	GSM379060 Ovary	GSM379061 Ovary	GSM379062 Ovary	GSM379063 Ovary	GSM379064 Ovary	GSM379065 Ovary	GSM379066 Ovary	GSM379067 Ovary	GSM424739 S2	GSM424740 S2	GSM424741 S2	GSM467729 Female-body	GSM467730 Female-body	GSM467731 Female-body	M002 CS	M003 Male-body	M004 Male-body	M005 ML-DmD32	M006 ML-DmD32	M029 OSC	M030 OSC	M031 OSC	M032 S2-R+	M033 S2-R+	M034 S2-R+	M035 S2-R+	M036 S2-R+	M037 S2-R+	M038 S2-R+	M039 S2-R+	M048 S2-R+	OSS2 OSS	OSS6 OSS	OSS7 OSS	OSS8 OSS	S1 Head	S10 Embryo	S11 Embryo	S12 Embryo	S13 Embryo	S14 Embryo	S15 S2-GMR	S16 Kc	S17 Larvae	S18 Larvae	S19 Pupae	S2 Head	S20 Pupae	S21 Disc	S22 Head	S23 Female-body	S24 Male-body	S25 Larvae	S26 Larvae	S27 Pupae	S28 Pupae	S29 Head	S3 Male-body	S30 S2-GMR	S31 S2	S32 S2	S33 Kc	S34 Kc	S35 Kc	S4 Female-body	S5 Disc	S6 Embryo	S7 Embryo	S8 S2	S9 Embryo	SRR001337 Head	SRR001338 Head	SRR001339 Head	SRR001340 Head	SRR001341 Head	SRR001342 Head	SRR001343 Head	SRR001344 Head	SRR001345 Head	SRR001346 Head	SRR001347 Head	SRR001348 Head	SRR001349 Head	SRR001664 Head	SRR010951 Ovary	SRR010952 Ovary	SRR010953 Ovary	SRR010954 Ovary	SRR010955 Ovary	SRR010956 Ovary	SRR010957 Ovary	SRR010958 Ovary	SRR010959 Ovary	SRR010960 Ovary	SRR014268 Embryo	SRR014269 Embryo	SRR014270 Embryo	SRR014271 Embryo	SRR014272 Embryo	SRR014273 Ovary	SRR014274 Ovary	SRR014275 Ovary	SRR014276 Ovary	SRR014277 Ovary	SRR014278 Ovary	SRR014279 Ovary	SRR014280 Ovary	SRR014281 Ovary	SRR014282 Ovary	SRR023197 S2	SRR023399 S2	SRR023400 S2	SRR023402 S2	SRR023407 S2	SRR029028 S2	SRR029029 S2	SRR029030 S2	SRR029031 S2	SRR029032 S2	SRR029033 S2	SRR029608 Head	SRR029633 Head	SRR030145 gb3	SRR031692 Head	SRR031693 Head	SRR031694 Head	SRR031696 Head	SRR031697 Head	SRR031698 Head	SRR031699 Head	SRR031700 Head	SRR031701 Head	SRR031702 Head	SRR031703 Head	SRR031704 Head	SRR032092 S2	SRR032093 S2	SRR032094 S2	SRR032095 S2-NP	SRR032096 S2-NP	SRR032152 Head	SRR060643 Testis	SRR060644 Ovary	SRR060645 Testis	SRR060646 Ovary	SRR060647 Ovary	SRR060648 Ovary	SRR060649 Ovary	SRR060650 Testis	SRR060651 Ovary	SRR060652 Testis	SRR060653 Ovary	SRR065151 Ovary	SRR065152 Ovary	SRR065153 Ovary	SRR065154 Ovary	SRR065155 Ovary	SRR065800 Ovary	SRR065801 Ovary	SRR065802 Ovary	SRR065803 Ovary	SRR065804 Ovary	SRR065805 Ovary	SRR065806 Ovary	SRR065807 Ovary	SRR097865 S2-NP	SRR097866 S2-NP	SRR097867 S2-NP	SRR298536 Ovary	SRR298537 Ovary	SRR298711 Ovary	SRR298712 Ovary	SRR317110 Ovary	SRR317111 Ovary	SRR317112 Ovary	SRR317113 Ovary	SRR317114 Ovary	SRR317115 Ovary	SRR317116 Ovary	SRR317117 Ovary	SRR341115 S2	SRR341116 S2	SRR341117 S2	SRR341118 S2	SRR351332 Mixed-body	SRR351333 Mixed-body	T004 OSC	T005 CME-L1	V001 Embryo	V002 Embryo	V003 S2	V006 Female-body	V007 Head	V008 S2	V009 CME-L1	V010 ML-DmD20-C5	V011 Sg4	V012 Male-body	V013 Head	V014 DTT	V015 DreRFHV	V018 Kc167	V019 GM2	V020 S2-R+	V021 ML-DmD21	V022 ML-DmD32	V023 Head	V024 Kc167	V025 S2-R+	V026 1182-2H	V027 ML-DmD21	V028 CME-W1-Cl.8+	V029 ML-DmD9	V030 ML-DmD8	V031 GM2	V032 S1	V033 CME-W1-Cl.8+	V034 ML-DmD16-C3	V035 ML-DmD32	V036 ML-DmD20-C5	V037 S2	V038 S2	V063 Ovary	V0632 Ovary	V064 Ovary	V0642 Ovary	V065 Ovary	V0652 Ovary	V066 Ovary	V0662 Ovary	V067 S2-NP	V073 mbn2	V074 S3	V075 ML-DmBG1-C1	V076 ML-DmBG3-C2	V077 Head	V078 Head	V079 Head	V080 Head	V081 Embryo	V082 Embryo	V083 Male-body	V084 Female-body	V085 CME-W2	V086 Female-body	V088 Ovary	V0882 Ovary	V089 Ovary	V0892 Ovary	V090 Male-body	V091 fGS/OSS	V092 Male-body	V093 Male-body	V094 S2-R+	V095 S2-R+	V096 Ovary	V097 Ovary	V098 Ovary	V125 ML-DmD9	V126 CME-L1	V127 G2	V128 S3	V129 ML-DmBG1-C1	V130 ML-DmBG3-C2	V131 ML-DmD16-C3	V132 ML-DmD32	V133 Sg4	V134 ML-DmD8	V135 CME-W2	V136 Male-body	V137 Head	V138 Male-body	V139 Female-body	V140 Female-body	V141 Female-body	V142 Female-body	V144 OSC	V145 S2	V146 S1	V147 1182-2H	V148 mbn2	GSM1528798 follicle cells
.......GCAACGTTAGGAACGGCCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	11.00	11	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	2	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	1	0	0	0	0	0	0
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...........................................................................................................................................GCTCACTTTCGCTCTGGGTGGTA.............................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
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droSim2

CTAGATCGCAACGTAAGGAACGGCCTGGGCGACCAGATAGCCTACTACTGGTGAGTTCGGT--T------GCA-----------------------------------------------------------AATCCTCGCCCACTTTCGCTCTGGGTGGCGTAGGTCGCACGAAAACAAAAGTATTT-GGGTGGCAGC----------------------------------------------------------------GCAGGA--TTATTGATTTTTC------------------------------GTCTGGTTACGAAACTCTCGCCTTGTGACGTAGAAA----------------------------------------------------------------------------------------GGTGGGCTCCTCTTGCACAGGATTTACTGCGCAGGCAC---	Size	Hit Count	Total Norm	Total	M023 Head	M024 Male-body	M025 Embryo	M053 Female-body	O001 Testis	O002 Head	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary	V044 Embryo	SRR902008 Ovary	SRR902009 Testis	SRR1275487 Male larvae	SRR1275485 Male prepupae	SRR1275483 Male prepupae
...GATCGCAACGTAAGGAAC..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	3.00	3	0	2	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................................................................................................CTGGTTACGAAACTCTCGCCT..............................................................................................................................................	21	1	3.00	3	2	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
........................................................................................................................................................................................................................................................................................TTTC------------------------------GTCTGGTTACGAAACTC....................................................................................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................................................................................................................TGTGACGTAGAAA----------------------------------------------------------------------------------------GGTGGGCTCCTCT............................	26	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................................................................................................................GGTTACGAAACTCTCGCCTT.............................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................GACCAGATAGCCTACTACTG.............................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....ATCGCAACGTAAGGAACGGCCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................................................................................................CTGGTTACGAAACTCTCGCCC..............................................................................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...GATCGCAACGTAAGGAACGGCCTGGG..................................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
.TAGATCGCAACGTAAGGAACGGCCTG....................................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
...GATCGCAACGTAAGGAACGGCCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0
....................CGGCCTGGGCGACCAGATAGCCTACTACTGGGA..........................................................................................................................................................................................................................................................................................................................................................................................................................................	33	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
..................................................GTGAGTTCGGT--T------GCA-----------------------------------------------------------AATCCT.....................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................................................TTATTGATTTTTC------------------------------GTCTGGTTT............................................................................................................................................................	22	2	0.50	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................................................................................................................GTTACGAAACTCTCT.................................................................................................................................................	15	14	0.07	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0

droSec2

CTAGATCGCAACGTAAGGAACGGCCTGGGCGACCAGATAGCCTACTACTGGTGAGTTCGGT--T------GCA-----------------------------------------------------------AATCCTCGCCCACTTTCGCTCTGGGTGGCGTAGGTCGTACGAAAACAAAAGTATTT-GTGTGGCAGC----------------------------------------------------------------GCAGGA--TTATTGATTTTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
...GATCGCAACGTAAGGAACGGCCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	27	1	25.00	25	2	10	13	0
.......GCAACGTAAGGAACGGCCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	9.00	9	1	2	6	0
...GATCGCAACGTAAGGAAC..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	3.00	3	0	0	3	0
.....TCGCAACGTAAGGAACGGCCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	25	1	3.00	3	0	1	2	0
..........ACGTAAGGAACGGCCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	2.00	2	0	1	1	0
..................................................................................................................................................TTCGCTCTGGGTGGCGTAGGTCG......................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	1	0	0	0
........CAACGTAAGGAACGGCCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	1	0
..........................................................................................................................................................GGGTGGCGTAGGTCGTACGAAA...............................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	1	0
......CGCAACGTAAGGAACGGC.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	1	0
.......................................................................................................................................................TCTGGGTGGCGTAGGTCGTAC...................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	1
...........CGTAAGGAACGGCCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	1	0
..................................AGATAGCCTACTACTGGGA..........................................................................................................................................................................................................................................................................................................................................................................................................................................	19	3	0.33	1	0	0	1	0

droYak3

CTGGATCGCAACGTGAGGAACGGTCTGGGCGACCAGATAGCCTACTACTGGTGAGTCTGGT--T------GGA-----------------------------------------------------------AATCCTCGCCCACTTTCCCTGCGGGTGGCGTAGGTCGCACGAAAACAAAAGTATTT-AGGAGGCAGC----------------------------------------------------------------GCAGGA--TTATTGATTTTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M026 Head	M043 Female-body	M056 Embryo	V046 Embryo	V052 Head	V058 Head	V120 Male-body	SRR1275488 Male larvae	SRR1275486 Male prepupae	SRR1275484 Male prepupae	GSM1528802 follicle cells
.......GCAACGTGAGGAACGGTCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	2	3.00	6	0	0	0	0	0	0	6	0	0	0	0
...GATCGCAACGTGAGGAACGGTCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	27	2	1.50	3	1	0	0	0	0	0	2	0	0	0	0
......CGCAACGTGAGGAACGGTCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	24	2	1.00	2	0	0	1	0	0	0	1	0	0	0	0
..........ACGTGAGGAACGGTCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	2	1.00	2	0	0	2	0	0	0	0	0	0	0	0
...GATCGCAACGTGAGGAACGGT.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	2	1.00	2	0	0	0	0	0	0	2	0	0	0	0
............................GCGACCAGATAGCCTACTACT..............................................................................................................................................................................................................................................................................................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0	0
......CGCAACGTGAGGAACGGTCTG....................................................................................................................................................................................................................................................................................................................................................................................................................................................................	21	2	0.50	1	0	1	0	0	0	0	0	0	0	0	0
.........AACGTGAGGAACGGTCTGGGCGACC.............................................................................................................................................................................................................................................................................................................................................................................................................................................................	25	2	0.50	1	0	0	1	0	0	0	0	0	0	0	0
....ATCGCAACGTGAGGAACGGTCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	2	0.50	1	0	1	0	0	0	0	0	0	0	0	0
.TGGATCGCAACGTGAGGAACGGTC......................................................................................................................................................................................................................................................................................................................................................................................................................................................................	24	2	0.50	1	0	0	0	0	0	0	1	0	0	0	0

droEre2

CTAGATCGCAACGTTAGGAACGGTCTGGGCGACCAGATAGCCTACTACTGGTGAGTCTGGT--T------GGA-----------------------------------------------------------AATCCTCGCCCACTTTCCCTCTGGGTGGCGTAGGTCGAACGAAAACAAAAGTATTT-ATGTGGTAGC----------------------------------------------------------------GCAGGA--TTATTGATTTTT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
.......GCAACGTTAGGAACGGTCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	11.00	11	0	4	1	1	3	2	0
...GATCGCAACGTTAGGAACGGTCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	27	1	4.00	4	0	3	0	0	0	1	0
...GATCGCAACGTTAGGAACG.........................................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	3.00	3	0	1	0	1	0	1	0
....ATCGCAACGTTAGGAACGGTCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	1	0	0	0	0	0	0
.......GCAACGTTAGGAACGGTCTGGGCGAC..............................................................................................................................................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	1	0	0
............GTTAGGAACGGTCTGGGCGACCA............................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	1	0	0
...........CGTTAGGAACGGTCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0
..........ACGTTAGGAACGGTCTGGGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0

dvi_20783

scaffold_13049:20519005-20519155 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-15.2	-15.1	-14.9