dvi_15595

CDS [Dvir\GJ23876-cds]; exon [dvir_GLEANR_9198:3]; exon [dvir_GLEANR_9198:2]; CDS [Dvir\GJ23876-cds]; intron [Dvir\GJ23876-in]

Sense Strand Reads

Anti-sense strand reads

##################################################---------------------------------------------------------################################################## GCCCCTAATACTCCAAACTGTCCACTTCTCAAAGTCTAAAGGCAGCGATGGTAAGAGATAATATAACTTAACATAAGAGAATTCACGTTGACAAAAACCTTTTGTAGGTGAGGTACGTTTAGGAAATGATGGCAAAAAACTAGCTGACGGTAAAAAG ************************************************......................(((((((.......(((......))))))))))..************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR060680 9xArg_testes_total	M047 female body	SRR060679 140x9_testes_total	V116 male body
.................CTGTCCACTTCTCAAAGTCTAAAGGC..................................................................................................................	26	0	1	1.00	1	1	0	0	0
........................ATTCGCAGAGTCTAAAGGCA.................................................................................................................	20	3	6	0.17	1	0	1	0	0
.......................................................................................................GATGGTGAGGTACGTTTA....................................	18	2	6	0.17	1	0	0	1	0
........................ATTCGCAGAGTCTAAAGGC..................................................................................................................	19	3	20	0.05	1	0	0	0	1

CGGGGATTATGAGGTTTGACAGGTGAAGAGTTTCAGATTTCCGTCGCTACCATTCTCTATTATATTGAATTGTATTCTCTTAAGTGCAACTGTTTTTGGAAAACATCCACTCCATGCAAATCCTTTACTACCGTTTTTTGATCGACTGCCATTTTTC ************************************************......................(((((((.......(((......))))))))))..************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M027 male body	V047 embryo	SRR060663 160_0-2h_embryos_total
.........................................CGTGGCTTCCATTCTCTA..................................................................................................	18	2	1	1.00	1	1	0	0
..........................................................................................TGTTTTTGGTATACATCAACTCCAT..........................................	25	3	1	1.00	1	0	1	0
....................................................................................TGGAACTGTTATCGGAAAAC.....................................................	20	3	9	0.11	1	0	0	1
.........TGAGGATTTACAGGTGAGG.................................................................................................................................	19	3	12	0.08	1	0	0	1

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droVir3	scaffold_13047:4932586-4932742 +	dvi_15595	GCCCCTAATACTCCAA--ACTGTCCACTTCTCAAAGTCTAAAGGCAGCGATGGTAAGAGATAA--TATAA---------------CTTAACATAAGAGAAT-TCACGTTGACAAAAACCTTTTGTAGGTGAGGTACGTTTAGGAAATGATGGCAAAAAACTAGCTGACGGTAAAAAG
droMoj3	scaffold_6540:21479910-21479965 -		GCTGTCAATATTGCAA--ATCGCGCACTACTCGAAGGCGAAAGGAGGCGACAGCAAGA-----------------------------------------------------------------------------------------------------------------------
droGri2	scaffold_14906:10363408-10363504 +		GCCGTTTGTACTTAGT--ACTGTGCACTACTCGAAGTCCAAAGGAAGCGACTGTGATAAAAAACCTTTTAAGGGAAACAAAGAACTTCTAAATCGAA-------------------------------------AA-----------------------------------------
droWil2	scf2_1100000004967:1120119-1120176 -		AATGTTGAAA--------AACGTAAAACTGCCAAAATTCAAAGGCAATGTTGGTAAAAGTTGA--T--------------------------------------------------------------------AT-----------------------------------------
droEle1	scf7180000489069:45464-45497 +		AAC-----------------AGTCCACTTGCCAAAGTTTACAAGCAGCA-------------------------------------------------------------------------------------AT-----------------------------------------
droRho1	scf7180000778317:12298-12351 -		AA----------------ACTGTCCGTTTCACAAGGTCTAAGACAAGCCCTACTACAAGATAACC-------------------------------ATA-----------------------------------AC-----------------------------------------
droTak1	scf7180000415769:1009318-1009375 -		TAAAAAAAAA-------------------------------------------------------TCTGA---------------AATAAAACAAGAGAAT-TAATATTTTCAAAAATATTATAGAG-------GT-----------------------------------------
droEug1	scf7180000405347:25209-25246 -		TTTTGTAG----------GTCATGCACTTTTGGGAGATT----------ATGGTAG------------------------------------------------------------------------------AA-----------------------------------------
droSim2	2l:22468965-22469040 -		GCCTTTCATAAGCCAACTAATGTGT-------AATAGTTAAATATAGC-ATAATAAAATATAA--TATAA---------------TTTAACATAATATA-----------------------------------AT-----------------------------------------
droYak3	v2_chr3L_random_018:24843-24920 +		AAAGGCAACA----------------CGTATAAAAAATAAAATAA----------------------------------------CGTCACATAAAAAAATGTCGCGTT--CAAAACCATATTTTGGCTAAATTAC-----------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droVir3

GCCCCTAATACTCCAA--ACTGTCCACTTCTCAAAGTCTAAAGGCAGCGATGGTAAGAGATAA--TATAA---------------CTTAACATAAGAGAAT-TCACGTTGACAAAAACCTTTTGTAGGTGAGGTACGTTTAGGAAATGATGGCAAAAAACTAGCTGACGGTAAAAAG	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
...................CTGTCCACTTCTCAAAGTCTAAAGGC....................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droMoj3

GCTGTCAATATTGCAA--ATCGCGCACTACTCGAAGGCGAAAGGAGGCGACAGCAAGA-----------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
No Reads

droGri2

GCCGTTTGTACTTAGT--ACTGTGCACTACTCGAAGTCCAAAGGAAGCGACTGTGATAAAAAACCTTTTAAGGGAAACAAAGAACTTCTAAATCGAA-------------------------------------AA-----------------------------------------	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

droWil2

AATGTTGAAA--------AACGTAAAACTGCCAAAATTCAAAGGCAATGTTGGTAAAAGTTGA--T--------------------------------------------------------------------AT-----------------------------------------	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
.....................GTAAAACTGCCAAAATTCAAAGGCA...................................................................................................................................	25	7	0.14	1	0	1	0	0	0
............................TGCCAAAATTCAAAGGCAATGTTGGTA..........................................................................................................................	27	8	0.13	1	0	1	0	0	0
.........................AACTGCCAAAATTCAAAGGCAATGTTGG............................................................................................................................	28	8	0.13	1	0	1	0	0	0
.....................................TCAAAGGCAATGTTGGTAAAAGTTG...................................................................................................................	25	20	0.10	2	0	0	0	2	0
....................................TTCAAAGGCAATGTTGGTAAAAGT.....................................................................................................................	24	20	0.10	2	0	0	0	2	0
....................................TTCAAAGGCAATGTTGGTAAAAGTTG...................................................................................................................	26	20	0.05	1	0	1	0	0	0
....................................TTCAAAGGCAATGTTGGTAAAAGTTGA--................................................................................................................	27	20	0.05	1	0	0	0	1	0

droEle1

AAC-----------------AGTCCACTTGCCAAAGTTTACAAGCAGCA-------------------------------------------------------------------------------------AT-----------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droRho1

AA----------------ACTGTCCGTTTCACAAGGTCTAAGACAAGCCCTACTACAAGATAACC-------------------------------ATA-----------------------------------AC-----------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droTak1

TAAAAAAAAA-------------------------------------------------------TCTGA---------------AATAAAACAAGAGAAT-TAATATTTTCAAAAATATTATAGAG-------GT-----------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEug1

TTTTGTAG----------GTCATGCACTTTTGGGAGATT----------ATGGTAG------------------------------------------------------------------------------AA-----------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droSim2

GCCTTTCATAAGCCAACTAATGTGT-------AATAGTTAAATATAGC-ATAATAAAATATAA--TATAA---------------TTTAACATAATATA-----------------------------------AT-----------------------------------------

Size

Hit Count

Total Norm

Total

M023
Head

M024
Male-body

M025
Embryo

M053
Female-body

O001
Testis

O002
Head

SRR553485
Ovary

SRR553486
Ovary

SRR553487
Ovary

SRR553488
Ovary

SRR618934
Ovary

V044
Embryo

SRR902008
Ovary

SRR902009
Testis

SRR1275487
Male larvae

SRR1275485
Male prepupae

SRR1275483
Male prepupae

No Reads

droYak3

AAAGGCAACA----------------CGTATAAAAAATAAAATAA----------------------------------------CGTCACATAAAAAAATGTCGCGTT--CAAAACCATATTTTGGCTAAATTAC-----------------------------------------	Size	Hit Count	Total Norm	Total	M026 Head	M043 Female-body	M056 Embryo	V046 Embryo	V052 Head	V058 Head	V120 Male-body	SRR1275488 Male larvae	SRR1275486 Male prepupae	SRR1275484 Male prepupae	GSM1528802 follicle cells
.....................................TAAAATAA----------------------------------------CGTCACATAAAAAAATGT..........................................................................	26	2	2.50	5	0	4	1	0	0	0	0	0	0	0	0
....................................................................................................TGTCGCGTT--CAAAACCATATTTTGGC.................................................	26	2	2.50	5	0	4	1	0	0	0	0	0	0	0	0
.....................................TAAAATAA----------------------------------------CGTCACATAAAAAAATG...........................................................................	25	2	1.00	2	0	2	0	0	0	0	0	0	0	0	0
.......................................................................................TCACATAAAAAAATGTCGCGTT--CAAAAC............................................................	28	2	1.00	2	0	1	1	0	0	0	0	0	0	0	0
......................................................................................................TCGCGTT--CAAAACCATATTTTGGC.................................................	24	2	0.50	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................TAAAAAAATGTCGCGTT--CAAAACCA..........................................................	25	2	0.50	1	0	1	0	0	0	0	0	0	0	0	0
.........................................................................................ACATAAAAAAATGTCGCGTT--CAAAACCA..........................................................	28	2	0.50	1	0	1	0	0	0	0	0	0	0	0	0
..........................................................................................CATAAAAAAATGTCGCGTT--CAAAACCA..........................................................	27	2	0.50	1	0	1	0	0	0	0	0	0	0	0	0
...................................................................................................ATGTCGCGTT--CAAAACCATATTTTGGC.................................................	27	2	0.50	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................TAAAAAAATGTCGCGTT--CAAAACCATA........................................................	27	2	0.50	1	0	1	0	0	0	0	0	0	0	0	0
..............................TAAAAAATAAAATAA----------------------------------------CGTCACATAAA.................................................................................	26	2	0.50	1	0	1	0	0	0	0	0	0	0	0	0
....................................................................................................TGTCGCGTT--CAAAACCATATTTTGGCT................................................	27	2	0.50	1	0	1	0	0	0	0	0	0	0	0	0

dvi_15595

scaffold_13047:4932636-4932692 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads