dvi_15594

ATCCATGTGTAATTATGTGACAACTATTAACATGTATGTAAATATGGGAGAAAAAAGCGAGCATAACCCATAACCTATACAGTTCTGAGTCAAGCGTTCTTATATGGTATATATGTATTTGATGAGGTTTATATATATAAACCGTATTTCGAGAGCTACGATAAAAGATTTGAATGAAAGCAAAGATTTGAAGAGAAGGTCCGCTATATCTGTCGGATAACTTTTTCAGTCTTGTCGGTTAATATGTTGTT ************************************************....(((...((.(((((((.((((.....)))).))))))).))...)))........................(((((((....))))))).....(((.......))).......((((........)))).................************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR060689 160x9_testes_total	SRR060659 Argentina_testes_total	SRR060670 9_testes_total	V116 male body	SRR060668 160x9_males_carcasses_total	M047 female body
.............................................................................................................................................................................................GTAGAGAAGGTCCGCTAT............................................	18	1	1	1.00	1	1	0	0	0	0	0
..............................................................................................................................................................................................AAGAGAAGGCCCGCGATA...........................................	18	2	2	0.50	1	0	1	0	0	0	0
.........................................................CGAGCATAGGCCATAATCT...............................................................................................................................................................................	19	3	6	0.17	1	0	0	1	0	0	0
................................................AGAAAACAGCGAGTATAGCC.......................................................................................................................................................................................	20	3	8	0.13	1	0	0	0	1	0	0
.............................................................................................................................................................................................GAAGAGAAGGTCTTCTATC...........................................	19	3	15	0.07	1	0	0	0	0	1	0
.........................................ATATGGGTGAAAAGAGGGAG..............................................................................................................................................................................................	20	3	18	0.06	1	0	0	0	0	0	1

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droVir3	scaffold_13047:4888067-4888317 -	dvi_15594	TAGGTACACATTAATACACTGTTGATAATTGTACATACATTTATACCCTCTTTTTTCGCTCGTATTGGGTATTGGATATGTCAAGACTCAGTTCGCAAGAATATACCATATATACATAAACTACTCCAAATATATATATTTGGCATAAAGCTCTCGATGCTATTTTCTAAACTTACTTTCG----TTTCTAA-A----CTTCTCTTCCAGGCGATATAGACAGCCTATTGAAAAAGTCAGAACAGCCAATTATACAACAA
droMoj3	scaffold_6540:21520924-21521003 +		AG----------------------------------------------------------------------------------------------------------------------------------------------------------------------AACTAAT-TTCACT-------------TATCTCACTTTCCAGGCGATTTAGACAGCCTCCTCAAAAAGTCGGAAAAGCCGATAATACAACAG
droGri2	scaffold_14906:10320959-10321046 -		TC----------------------------------------------------------------------------------------------------------------------------------------------------------------------GAGGCAT-TTAGCTAAAC--T---TTTTTCCTGACTTTCCAGGCGATATTGGCAACCTGTTAAAAACCTCCGAGGCGCCCATTATCCAACAA
droWil2	scf2_1100000004943:7722664-7722719 +		T------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTCAGCCAGTACCGATAATCTATTGGAGACTTCTGAAAAGCCAATTTTGAATCAG
dp5	2:28495164-28495219 +		T------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCCAGGCAACATAGCCAACCTCTTGACCAGCTCTAAGCAACCGATTATTAAGCAA
droPer2	scaffold_6:3825638-3825693 +		T------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCCAGGCAACATAGCCAACCTATTGACCAGCTCTAAGCAACCGATTATTAAGCAA
droAna3	scaffold_13340:12156164-12156225 -		C------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTCTATTTTAGGCAGTATGTCCAACCTGTTGGAATCGTCAAAGGAACCGATAATTAAACAG
droKik1	scf7180000302650:415164-415248 +		AT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTACTTTAT----TTATCAA-ATTTATAACAATTCCAGGAAGAATGAGCAACTTGCTGGAGAGTTCCAAGCAGCCGATAATGAAGCAG
droEle1	scf7180000491008:2726427-2726508 -		CT----------------------------------------------------------------------------------------------------------------------------------------------------------------------TATCCTA---------TTTGC---GTTTCCCTACAACTTTAGGAAGAATGGCCAACTTATTGGAATCATCAAAAAAGCCTATTATCGAACAG
droRho1	scf7180000760575:18775-18883 -		ATATTAGT----------------------------------------------------------------------------TTCTTAATTCAAGA-----------------------------------------------------------------------AAATAC-TTACCTTGTTTAC---TTTTTCTTAAAATTGAAGGAAGAATGGCAAACTTATTGGAATCATCAAAAAGACCTATAATCGAACAA
droEug1	scf7180000409797:600889-600944 +		T------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTCAGAAAGAATGGCCAACCTATTGGAATCATCCAAGGAGCCAATAATCAAGCAG

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droVir3

TAGGTACACATTAATACACTGTTGATAATTGTACATACATTTATACCCTCTTTTTTCGCTCGTATTGGGTATTGGATATGTCAAGACTCAGTTCGCAAGAATATACCATATATACATAAACTACTCCAAATATATATATTTGGCATAAAGCTCTCGATGCTATTTTCTAAACTTACTTTCG----TTTCTAA-A----CTTCTCTTCCAGGCGATATAGACAGCCTATTGAAAAAGTCAGAACAGCCAATTATACAACAA	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
.....................................................................................................................................................................................................................................GAAAAAGTCAGAACAGCCAATT.........	22	1	3.00	3	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................GATATAGACAGCCTATTGAAAAAGTC......................	26	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................................................................................................................................................................................................................AGCCTATTGAAAAAGTCAGAACAGC..............	25	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................GCCTATTGAAAAAGTCAGAACAGC..............	24	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................................................................................................................................................................................GACAGCCTATTGAAAAAGT.......................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................TATTGAAAAAGTCAGAACAGCCAATT.........	26	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droMoj3

AG----------------------------------------------------------------------------------------------------------------------------------------------------------------------AACTAAT-TTCACT-------------TATCTCACTTTCCAGGCGATTTAGACAGCCTCCTCAAAAAGTCGGAAAAGCCGATAATACAACAG	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
................................................................................................................................................................................................................................CTCCTCAAAAAGTCGGAAAAGCCGATA.........	27	1	1.00	1	0	0	0	0	0	1
.........................................................................................................................................................................ACTAAT-TTCACT-------------TATCTCACT........................................................	21	1	1.00	1	0	0	0	0	1	0
......................................................................................................................................................................................................................TTTAGACAGCCTCCTCAAAAA.........................	21	1	1.00	1	1	0	0	0	0	0

droGri2

TC----------------------------------------------------------------------------------------------------------------------------------------------------------------------GAGGCAT-TTAGCTAAAC--T---TTTTTCCTGACTTTCCAGGCGATATTGGCAACCTGTTAAAAACCTCCGAGGCGCCCATTATCCAACAA	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
........................................................................................................................................................................................................................................AACCTCCGAGGCGCCCATTATCCAACA.	27	1	1.00	1	1	0

droWil2

T------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTCAGCCAGTACCGATAATCTATTGGAGACTTCTGAAAAGCCAATTTTGAATCAG	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
.............................................................................................................................................................................................................................AATCTATTGGAGACTTCTGAAAAGCCAA...........	28	1	1.00	1	1	0	0	0	0
................................................................................................................................................................................................................................CTATTGGAGACTTCTGAAAAGCC.............	23	1	1.00	1	0	1	0	0	0
................................................................................................................................................................................................................................CTATTGGAGACTTCTGAAAAGC..............	22	1	1.00	1	0	0	1	0	0
.........................................................................................................................................................................................................................CGATAATCTATTGGAGACTT.......................	20	1	1.00	1	0	0	1	0	0
..........................................................................................................................................................................................................................GATAATCTATTGGAGACTTCT.....................	21	1	1.00	1	0	0	1	0	0

dp5

T------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCCAGGCAACATAGCCAACCTCTTGACCAGCTCTAAGCAACCGATTATTAAGCAA	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
...........................................................................................................................................................................................................................................CTCTAAGCAACCGATTATTAAGCA.	24	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................................................................GACCAGCTCTAAGCAACCGA...........	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0

droPer2

T------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCCAGGCAACATAGCCAACCTATTGACCAGCTCTAAGCAACCGATTATTAAGCAA	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
................................................................................................................................................................................................................................CTATTGACCAGCTCTAAGC.................	19	1	1.00	1	0	0	1	0	0	0
.......................................................................................................................................................................................................................................CCAGCTCTAAGCAACCGATT.........	20	1	1.00	1	0	0	1	0	0	0
..................................................................................................................................................................................................................................ATTGACCAGCTCTAAGCAACC.............	21	1	1.00	1	0	1	0	0	0	0

droAna3

C------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTCTATTTTAGGCAGTATGTCCAACCTGTTGGAATCGTCAAAGGAACCGATAATTAAACAG	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
............................................................................................................................................................................................................................CAACCTGTTGGAATCGTCAAAG..................	22	1	2.00	2	1	0	1	0	0	0
.............................................................................................................................................................................................................................AACCTGTTGGAATCGTCAAAGGA................	23	1	1.00	1	0	1	0	0	0	0

droKik1

AT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTACTTTAT----TTATCAA-ATTTATAACAATTCCAGGAAGAATGAGCAACTTGCTGGAGAGTTCCAAGCAGCCGATAATGAAGCAG	Size	Hit Count	Total Norm	Total
No Reads

droEle1

CT----------------------------------------------------------------------------------------------------------------------------------------------------------------------TATCCTA---------TTTGC---GTTTCCCTACAACTTTAGGAAGAATGGCCAACTTATTGGAATCATCAAAAAAGCCTATTATCGAACAG	Size	Hit Count	Total Norm	Total
No Reads

droRho1

ATATTAGT----------------------------------------------------------------------------TTCTTAATTCAAGA-----------------------------------------------------------------------AAATAC-TTACCTTGTTTAC---TTTTTCTTAAAATTGAAGGAAGAATGGCAAACTTATTGGAATCATCAAAAAGACCTATAATCGAACAA	Size	Hit Count	Total Norm	Total
No Reads

droEug1

T------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTCAGAAAGAATGGCCAACCTATTGGAATCATCCAAGGAGCCAATAATCAAGCAG	Size	Hit Count	Total Norm	Total
No Reads

dvi_15594

scaffold_13047:4888117-4888267 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------################################################## TAGGTACACATTAATACACTGTTGATAATTGTACATACATTTATACCCTCTTTTTTCGCTCGTATTGGGTATTGGATATGTCAAGACTCAGTTCGCAAGAATATACCATATATACATAAACTACTCCAAATATATATATTTGGCATAAAGCTCTCGATGCTATTTTCTAAACTTACTTTCGTTTCTAAACTTCTCTTCCAGGCGATATAGACAGCCTATTGAAAAAGTCAGAACAGCCAATTATACAACAA ************************************************....(((...((.(((((((.((((.....)))).))))))).))...)))........................(((((((....))))))).....(((.......))).......((((........)))).................************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR060674 9x140_ovaries_total	SRR060682 9x140_0-2h_embryos_total	SRR060656 9x160_ovaries_total	M061 embryo	SRR060655 9x160_testes_total	SRR060689 160x9_testes_total	SRR060663 160_0-2h_embryos_total	M047 female body	SRR060664 9_males_carcasses_total	SRR060672 9x160_females_carcasses_total	SRR060684 140x9_0-2h_embryos_total	V053 head	SRR060667 160_females_carcasses_total	SRR060686 Argx9_0-2h_embryos_total	M027 male body	SRR060673 9_ovaries_total	V116 male body
.....................................................................................................................................................................................................................GCCTGTTGAAAAAGTCGGA...................	19	2	2	8.00	16	0	7	0	0	1	4	2	0	0	0	1	1	0	0	0	0	0
...................................................................................................................................................................................................................................TCAGAACAGCCAATAATGC.....	19	2	1	7.00	7	7	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................GAAAAAGTCAGAACAGCCAATT.........	22	0	1	3.00	3	0	0	0	0	2	0	0	0	0	1	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................AATCAGAACAGCCAATAATGC.....	21	3	5	2.80	14	4	0	10	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................CGCCTGTTGAAAAAGTCGGA...................	20	3	9	1.56	14	0	2	0	0	0	3	6	0	0	0	2	0	0	0	1	0	0
....................................................................................................................................................................................................................................CAGAACAGCCAATAATGC.....	18	2	9	1.44	13	6	0	7	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................................................................................................................................................................................................GATATAGACAGCCTATTGAAAAAGTC......................	26	0	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................AGCCTATTGAAAAAGTCAGAACAGC..............	25	0	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................................................GCCTATTGAAAAAGTCAGAACAGC..............	24	0	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................GACAGCCTATTGAAAAAGT.......................	19	0	1	1.00	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0
........................................................................................................................................................................................................................TATTGAAAAAGTCAGAACAGCCAATT.........	26	0	1	1.00	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................................TCAGAACAGCCAATAATGCCA...	21	3	2	0.50	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................................TCAGAACAGCCAATAATGCC....	20	3	8	0.38	3	1	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................................AAATCAGAACAGCCAATAATGC.....	22	3	4	0.25	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0
................................................................................................................................................................................................................................AAATCAGAACAGCCAATAAT.......	20	2	4	0.25	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................GCATTGGATACGTCCAGACT...................................................................................................................................................................	20	3	5	0.20	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................................................................................................................................................................CCTGTTGAAAAAGTCGGA...................	18	2	5	0.20	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................................................................................................................................................................CTTAATTTCGTTCCTAGACT............................................................	20	3	7	0.14	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
................................................................................................AAGAACATACCATATAGAGAT......................................................................................................................................	21	3	12	0.08	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1
....................................................................................................................................................................................................................AGCCTGTTGAAAAAGTCG.....................	18	2	12	0.08	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0
.....................................................................................................................................ATTTATTGGGCATAAAGC....................................................................................................	18	2	20	0.05	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0