dvi_14353

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------################################################## CACAAAGTATTTTTCATGGAAAACATTTTTTGGTCAACAGGTTGCTTTCAGGTTTGAACCGGCTTGTTTCACACGCGCCAGCACAATCTAGTTTGCGTTGCGATGTTTTCTCCTGGAAAATAATTTCAGGTCCGAGTCAACAAAGGAAATTTAGTAAATCGTCGAATGGATCTGAACAATATAAATAACATACTCTTGCAGAATCATGAGGAGAAGCGAAAGCTGAAGAAGAAGAGGAGGAGGAGGAAGAG ************************************************(((....)))((..((((((.((.((((.(((........))).)))))).))((((((((...))))))))..((((((((((..((.((...................)).)).))))))))))..........))))..)).......************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V116 male body	SRR060657 140_testes_total	SRR060667 160_females_carcasses_total	SRR060679 140x9_testes_total	SRR060658 140_ovaries_total	GSM1528803 follicle cells	SRR060655 9x160_testes_total	SRR060669 160x9_females_carcasses_total	M027 male body	SRR060687 9_0-2h_embryos_total	M047 female body	SRR060659 Argentina_testes_total	SRR060682 9x140_0-2h_embryos_total	SRR060684 140x9_0-2h_embryos_total
.....................................................................................................................................................................................................................................AGAAGAGGAGGAGGAGGAAGA.	21	0	1	1.00	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................................AAGAAGAGGAGGAGGAGGAAGA.	22	0	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................................AAGAAGAGGAGGAGGAGGAA...	20	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................CATGAGAAGAAGCGAAAGCTGAAG.......................	24	1	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0
........................................................................................................................................................................................................................................AGAGGAGGAGGAGGAAGA.	18	0	5	0.40	2	0	0	0	0	2	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................AGAAGAAGAGGAGGAGGAAGAAGA.	24	1	3	0.33	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................................AAGAGGAGGAGGAGGAAG..	18	0	5	0.20	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................................AAGAGGAGGAGGAGGAAGA.	19	0	5	0.20	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0
................................................................................................................................................................................................................AGGGGAAGCGAAAGCTAA.........................	18	2	6	0.17	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0
...............................................................................................................................................................................................................................TGAAGAAGAAGAGGAGGA..........	18	0	20	0.15	3	2	0	0	0	0	0	0	0	0	0	0	1	0	0
.....................................................................................................GATATTTTCTCCTGG.......................................................................................................................................	15	1	20	0.15	3	0	0	0	0	0	3	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................................................................AGAAGAGGAGGAGGATGTAGC.	21	3	13	0.08	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0
...................................................................................................................................................................................................................................GAAGAAGAGGACGCGGATGAAGA.	23	3	19	0.05	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0
...................................................................................................................................................AATTCCGTGAATCGTCGAA.....................................................................................	19	3	19	0.05	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0
................................................................................................................................................................................................................................GAAAAAGAAGACGAGGAGGACG.....	22	3	20	0.05	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0
....................................................TTTGAACCGGGATGTTTGA....................................................................................................................................................................................	19	3	20	0.05	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0
......................................................................................................................................................................................................................................GTAAGGGAGGAGGAGGAAGA.	20	3	20	0.05	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0
...................................................................................................................................................AATTCAGTGAATCATCGAAT....................................................................................	20	3	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1
.....................................................................................................................................................TTCCGTGAATCGTCGAAT....................................................................................	18	3	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0
...............................................................................................................................................................................................................................TTTAGAAGAAGAGCAGGAGGA.......	21	3	20	0.05	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0
....................................................................................................CGATATTTTCTCCTCGAAA....................................................................................................................................	19	2	20	0.05	1	0	0	0	0	0	1	0	0	0	0	0	0	0	0
....................................................................................................................................................................................................................................AAGAAGAGGAGGAGGATGT....	19	2	20	0.05	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0

GTGTTTCATAAAAAGTACCTTTTGTAAAAAACCAGTTGTCCAACGAAAGTCCAAACTTGGCCGAACAAAGTGTGCGCGGTCGTGTTAGATCAAACGCAACGCTACAAAAGAGGACCTTTTATTAAAGTCCAGGCTCAGTTGTTTCCTTTAAATCATTTAGCAGCTTACCTAGACTTGTTATATTTATTGTATGAGAACGTCTTAGTACTCCTCTTCGCTTTCGACTTCTTCTTCTCCTCCTCCTCCTTCTC ************************************************(((....)))((..((((((.((.((((.(((........))).)))))).))((((((((...))))))))..((((((((((..((.((...................)).)).))))))))))..........))))..)).......************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V053 head	SRR1106721 embryo_10-12h	SRR060679 140x9_testes_total	SRR060682 9x140_0-2h_embryos_total
.............................................................CGGACAAAGTGTGCGGGGT...........................................................................................................................................................................	19	2	1	1.00	1	1	0	0	0
...................................................................................................................................................................................................................TCTTCCCTTTCGACTTTTCCTTC.................	23	3	1	1.00	1	0	1	0	0
............................................................TCGGCCAAAGTGTGCGCGGTC..........................................................................................................................................................................	21	3	2	0.50	1	1	0	0	0
.....................................................................................................CTACAAAAGAGGTGCTTATA..................................................................................................................................	20	3	10	0.10	1	0	0	1	0
................................CAGTTGTCCAACGAAAAG.........................................................................................................................................................................................................	18	2	10	0.10	1	0	0	0	1
.............................................................CGACGAAAGTGTGCGTGGTC..........................................................................................................................................................................	20	3	15	0.07	1	1	0	0	0

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droVir3	scaffold_13042:2009130-2009380 -	dvi_14353	CACAAAGTATTTTTCATGGAAAACATTTTTTGGTCAACAGGTTGCTTTCAGGTTTGAACCGGCTTGTTTCACACGCGCCAGCACAATCTAGTTTGCGTTGCGATGTTTTCTCCTGGAAAATAATTTCAGGTCCGAGTCAACAAAGGAAATTTAGTAAATCGTCGAATGGATCTGAACAATATAAATAACATACTCTTGCAGAATCATGAGGAGAAGCGAAAG----------CTGAAGAAGAAG-AGGAGGAGGAGGAAGAG
droMoj3	scaffold_6359:3902755-3902830 +		ACAA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TAAAAATGCAATAAACTGAAAGGGTCACAAGCCGCAGCAAAAG----------CAGAAGAAGAGG-AGGAAGAAGAAGAAGCG
droGri2	scaffold_14832:2545-2586 +		GAAG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ACGAAATGG----------ATGAGGAAGAGG-AGGAGGAGGAGGAGGAG
droWil2	scf2_1100000004515:854026-854073 +		CAAA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGGAAAAACA-----------------------------GTGG----------CTAAAAGAGAAGGAGGAGGAGGAGGAGCAG
droPer2	scaffold_4:3559742-3559767 -		AGGA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ACAGG-AGATGAAGGAGCAGCAG
droKik1	scf7180000302698:593000-593042 +		AGAG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAGGAGGAAG----------ACGAGGATGAGG-AGGAGGACGATGAAGAG
droFic1	scf7180000453839:2002029-2002095 -		GCGGAAA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAGGAAAACA----------------------GGAAACGAAACTTCAAGCCCAGCGAAGAAGAAG-ACAAGGAAGAACTAGAG
droRho1	scf7180000777356:7045-7094 +		CGGA------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ACCGGAAAGAGAA---GCGG----------CCGCAAGGGAAG-TAGAAGAGGAAGCAGAG
droEug1	scf7180000409474:440241-440290 -		AGCA---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCATGAGGAAGACGAGG----------ATGAGGAAGAGG-AGGAGGAGGAGGAGGAG
droYak3	3L:17455116-17455172 +		GCAG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AACCGGAAACGGAAGCGACAGTAG-------CAGGCCGGAAAC-AGGAGGAGGAGGAAAAA
droEre2	scaffold_4784:17212668-17212721 +		GCAG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AACCGGAAACGGAAGCGACAG----------TCGCACACCGGA-AACAGGAGGAGGAAAAA

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droVir3

CACAAAGTATTTTTCATGGAAAACATTTTTTGGTCAACAGGTTGCTTTCAGGTTTGAACCGGCTTGTTTCACACGCGCCAGCACAATCTAGTTTGCGTTGCGATGTTTTCTCCTGGAAAATAATTTCAGGTCCGAGTCAACAAAGGAAATTTAGTAAATCGTCGAATGGATCTGAACAATATAAATAACATACTCTTGCAGAATCATGAGGAGAAGCGAAAG----------CTGAAGAAGAAG-AGGAGGAGGAGGAAGAG	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
...............................................................................................................................................................................................................................................AGAAG-AGGAGGAGGAGGAAGA.	21	1	1.00	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................................AAGAAG-AGGAGGAGGAGGAAGA.	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................................AAGAAG-AGGAGGAGGAGGAA...	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0
..................................................................................................................................................................................................................................................AG-AGGAGGAGGAGGAAGA.	18	5	0.40	2	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................................................................................................................................................................................AAG-AGGAGGAGGAGGAAG..	18	5	0.20	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0
.................................................................................................................................................................................................................................................AAG-AGGAGGAGGAGGAAGA.	19	5	0.20	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0
.........................................................................................................................................................................................................................................TGAAGAAGAAG-AGGAGGA..........	18	20	0.15	3	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0

droMoj3

ACAA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TAAAAATGCAATAAACTGAAAGGGTCACAAGCCGCAGCAAAAG----------CAGAAGAAGAGG-AGGAAGAAGAAGAAGCG	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
No Reads

droGri2

GAAG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ACGAAATGG----------ATGAGGAAGAGG-AGGAGGAGGAGGAGGAG	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
.............................................................................................................................................................................................................................................GAAGAGG-AGGAGGAGGAGGAG...	21	13	0.08	1	0	1

droWil2

CAAA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGGAAAAACA-----------------------------GTGG----------CTAAAAGAGAAGGAGGAGGAGGAGGAGCAG	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
No Reads

droPer2

AGGA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ACAGG-AGATGAAGGAGCAGCAG	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
No Reads

droKik1

AGAG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAGGAGGAAG----------ACGAGGATGAGG-AGGAGGACGATGAAGAG	Size	Hit Count	Total Norm	Total
No Reads

droFic1

GCGGAAA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAGGAAAACA----------------------GGAAACGAAACTTCAAGCCCAGCGAAGAAGAAG-ACAAGGAAGAACTAGAG	Size	Hit Count	Total Norm	Total
No Reads

droRho1

CGGA------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ACCGGAAAGAGAA---GCGG----------CCGCAAGGGAAG-TAGAAGAGGAAGCAGAG	Size	Hit Count	Total Norm	Total
No Reads

droEug1

AGCA---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCATGAGGAAGACGAGG----------ATGAGGAAGAGG-AGGAGGAGGAGGAGGAG	Size	Hit Count	Total Norm	Total
No Reads

droYak3

GCAG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AACCGGAAACGGAAGCGACAGTAG-------CAGGCCGGAAAC-AGGAGGAGGAGGAAAAA

Size

Hit Count

Total Norm

Total

M026
Head

M043
Female-body

M056
Embryo

V046
Embryo

V052
Head

V058
Head

V120
Male-body

SRR1275488
Male larvae

SRR1275486
Male prepupae

SRR1275484
Male prepupae

GSM1528802
follicle cells

No Reads

droEre2

GCAG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AACCGGAAACGGAAGCGACAG----------TCGCACACCGGA-AACAGGAGGAGGAAAAA	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
..........................................................................................................................................................................................................................................GCACACCGGA-AACAGGAGGAG......	21	1	1.00	1	0	0	0	0	1	0	0
.........................................................................................................................................................................................................................................CGCACACCGGA-AACAGGAGGA.......	21	1	1.00	1	0	0	0	0	1	0	0

dvi_14353

scaffold_13042:2009180-2009330 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads